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Water table rise sustains carbon release from soils in wetland-dominated landscapes: An intact soil core study

Wetland-dominated landscapes influence carbon cycling through their potential to act as both carbon sinks and sources. Wetlands in low-relief landscapes have dynamic terrestrial-aquatic interfaces that change seasonally with variable surface water and groundwater levels. However, few studies have directly quantified dissolved organic matter (DOM) release and greenhouse gas (CO 2 , CH 4 ) fluxes from wetland soils along terrestrial-aquatic interfaces as they are seasonally re-saturated by groundwater. To estimate groundwater-mediated soil DOM and gas fluxes, we performed laboratory simulations of vertical groundwater rise on intact soil cores collected from four Delmarva bay wetlands located in the Mid-Atlantic United States. At each wetland, one core was collected from within the wetland basin and the other from the transitional zone at the basin edge. Cores were re-saturated with groundwater over 15 days and then kept fully saturated for an additional 25 days. Source groundwater, soil porewater, and exfiltrated surface water samples were collected and analyzed for pH, ORP, DOM concentration, and DOM optical indices. In both the wetland and transition zone cores, porewater DOM concentrations increased over the wet up and were sustained during prolonged saturation. Optical indices shifted from recently produced, microbial-like signatures towards aromatic, terrestrial-like signatures. Fluxes of CO 2 decreased as the duration of soil saturation increased and soil cores switched from CH 4 sinks to sources upon full soil core saturation. Results indicate that groundwater rise sustains carbon mobilization from soils in wetland-dominated landscapes, emphasizing the need to understand how climate-driven changes to groundwater dynamics may affect carbon fluxes along terrestrial-aquatic interfaces.

Delaware, Maryland

Ice sheet dynamics drive pronounced changes in the subsurface freshwater-saltwater interface

Saltwater is migrating into freshwater aquifers globally with water quality and biogeochemical implications, yet saltwater intrusion in glaciated regions is sparsely investigated. Field observations suggest that groundwater head in glaciated systems is influenced by ice sheet forcings and provides evidence that seawater infiltrated into offshore aquifers during past deglaciation events. To understand links between ice sheet dynamics, groundwater head, and saltwater intrusion, we use numerical models to explore the effects of deglaciation on nearshore head and salinity distributions. We find that ice sheet thinning diminishes groundwater head, and the resulting shift in subsurface pressure gradients drives rapid landward movement of the subsurface freshwater-saltwater interface up to 4.0 km or 1.3 m per m ice sheet loss. Results highlight an overlooked saltwater intrusion mechanism that aligns with field observations and affects glaciated coastlines undergoing ice sheet retreat, underscoring the need to consider this mechanism in studies of contemporary coastal water quality.

Geophysical Research Letters

On connecting hydro-social parameters to vegetation greenness differences in an evolving groundwater-dependent ecosystem

Understanding groundwater-dependent ecosystems (i.e., areas with a relatively shallow water table that plays a major role in supporting vegetation health) is key to sustaining water resources in the western United States. Groundwater-dependent ecosystems (GDEs) in Colorado have non-pristine temporal and spatial patterns, compared to agro-ecosystems, which make it difficult to quantify how these ecosystems are impacted by changes in water availability. The goal of this study is to examine how key hydrosocial parameters perturb GDE water use in time and in space. The temporal approach tests for the additive impacts of precipitation, surface water discharge, surface water mass balance as a surrogate for surface–groundwater exchange, and groundwater depth on the monthly Landsat normalized difference vegetation index (NDVI). The spatial approach tests for the additive impacts of river confluences, canal augmentation, development, perennial tributary confluences, and farmland modification on temporally integrated NDVI. Model results show a temporal trend (monthly, 1984–2019) is identifiable along segments of the Arkansas River at resolutions finer than 10 km. The temporal impacts of river discharge correlate with riparian water use sooner in time compared to precipitation, but this result is spatially variable and dependent on the covariates tested. Spatially, areal segments of the Arkansas River that have confluences with perennial streams have increased cumulative vegetation density. Quantifying temporal and spatial dependencies between the sources and effects of GDEs could aid in preventing the loss of a vulnerable ecosystem to increased water demand, changing climate, and evolving irrigation methodologies.

Colorado

The Hardscrabble Creek complex: A newly discovered, mostly buried, Mesoproterozoic mafic-ultramafic pluton in the Wet Mountains, Colorado, USA

The origin of prolific ca. 1.4 Ga ferroan magmatism between the southwestern USA and eastern Canada is enigmatic and has been explained by various models, including extensional, mantle plume, and convergent plate-margin models. Rare mafic plutons are associated with the ferroan plutons, which may help constrain their mantle source and tectonic setting. In the southwestern USA, only two such mafic plutons are known to exist. We present the first evidence for a third, mostly buried, potentially layered, mafic-ultramafic Mesoproterozoic pluton, informally referred to as the Hardscrabble Creek complex, in the central Wet Mountains of Colorado, USA. Recent geophysical data show an elliptical magnetic and gravity high spatially coincident with local gabbroic outcrops. New field and petrographic analyses of these exposed rocks reveal that they consist of ultramafic to mafic cumulates, including orthopyroxenite, olivine norite, norite, and anorthosite. High-precision U-Pb dating of zircon from orthopyroxenite and norite yield weighted mean 206 Pb/ 238 U dates of 1352.36 ± 1.60 Ma and 1352.37 ± 1.71 Ma, respectively. These dates indicate that the complex formed over a narrow timeframe, after the adjacent 1362 ± 7 Ma ferroan San Isabel Granite, and during the waning stages of the regional ca. 1.4 Ga ferroan magmatism. Whole-rock geochemistry and Nd-Sr-Pb isotope compositions of samples from the Hardscrabble Creek complex are similar to those of the San Isabel Granite, suggesting that they were derived from the same or a similar mantle source. The mineral chemistry of the samples is comparable to Proterozoic massif-type anorthosites and related mafic intrusions, indicating that the Hardscrabble Creek complex and San Isabel Granite together represent a rare anorthosite-mangerite-charnockite-granite (AMCG) suite in the southwestern USA. The Hardscrabble Creek complex is unique because it formed ~80 m.y. after the other few mafic plutons in the southwestern USA, and it contains an ultramafic section that is absent from these plutons and rare to the AMCG suite in general. A combination of arc-like whole-rock geochemistry, chondrite uniform reservoir-like Nd-Sr-Pb isotopes, and ocean island basalt (OIB)-like zircon trace element chemistry suggests that the complex was derived from a partial melt of OIB-like mantle and interacted with metasomatically enriched lithospheric mantle. The enriched lithospheric mantle signature, combined with the long ~160 m.y. duration of magmatism in the region, is consistent with a period of protracted convergent tectonism.

Colorado

Seismic velocity changes from repetitive seismicity at Mauna Loa prior to and during its 2022 eruption

Mauna Loa’s short-lived eruption from late November to early December 2022 marked the culmination of nearly a decade of elevated seismic activity and geodetic inflation. The volcano has been monitored by a network of permanent, short period and broadband seismometers. I used the continuous waveform data from that network starting in 2012 to generate a catalog of seismicity that enhances the US Geological Survey Hawaiian Volcano Observatory’s public seismic catalog with four times the number of earthquakes, which were then grouped by waveform similarity. Analysis of subtle delays in the timing of arrivals of scattered waves between pairs of earthquakes in this catalog yields a history of small changes in the shallow seismic velocity structure of the volcano. Seismic velocities have been shown at other volcanoes to change during unrest and eruption. My results show a decrease in seismic velocity centered on the summit beginning in September 2022, corresponding to the onset of a vigorous precursory swarm of seismic activity and shallow inflation. During the eruption itself, I observe large changes due likely to dike opening along the northeast rift zone and deflation of the summit reservoir. However, seismic velocity changes associated with non-volcanic sources such as ground shaking from large earthquakes and meteorological influences at seasonal and diurnal time scales are also observed, and these dominate the velocity changes prior to the eruption. Proper accounting of these effects will be a requirement for use in real-time monitoring, and this work serves as a starting point in that endeavor for Mauna Loa.

Hawaii

Estimating the importance of floating surface material to the total phosphorus transport in Silver Creek, Wisconsin using Particle Image Velocimetry

Various techniques are used to estimate nutrient delivery in streams that combine flow and water-quality data. However, the transport of surface floating material is difficult to measure, and is therefore typically neglected when stream sampling and in the estimated nutrient delivery. Here, we describe an approach to estimate the amount of material (duckweed ( Lemna genus), filamentous algae, and other macrophyte fragments) and associated nutrients (in this case, phosphorus, P) transported on the surface of Silver Creek, Wisconsin, to determine if this material is an important transport mechanism and if historical P loads were underestimated. This approach includes estimating the transport of surface material using 10 s videos collected every 15 min from a downward-looking camera installed beneath a bridge. The average velocity of the surface material was first determined using Large-Scale Particle Image Velocimetry (LSPIV), which uses short videos to analyze surface particle movement. The amount of surface material in each video was then computed using computer-vision techniques. The P load associated with the transported surface material was then estimated by combining surface velocities, coverage of floating material, and laboratory-measured P content. Surface material transported ~9–11% of the total summer P load and ~4–7% of the annual load in Silver Creek.

Wisconsin

Flow heterogeneity controls dissolution dynamics in topologically complex rocks

Rock dissolution is a common subsurface geochemical reaction affecting pore space properties, crucial for reservoir stimulation, carbon storage, and geothermal energy. Predictive models for dissolution remain limited due to incomplete understanding of the mechanisms involved. We examine the influence of flow, transport, and reaction regimes on mineral dissolution using 29 time-resolved data from 3D rocks. We find that initial pore structure significantly influences the dissolution pattern, with reaction rates up to two orders of magnitude lower than batch conditions, given solute and fluid-solid boundary constraints. Flow unevenness determines the location and rate of dissolution. We propose two models describing expected dissolution patterns and effective reaction rates based on dimensionless metrics for flow, transport, and reaction. Finally, we analyze feedback between evolving flow and pore structure to understand conditions that regulate/reinforce dissolution hotspots. Our findings underscore the major impact of flow arrangement on reaction-front propagation and provide a foundation for controlling dissolution hotspots.

Geophysical Research Letters

Revisiting the utility of regional-scale, high-quality geophysical data in mineral exploration - A case study featuring the Mammoth Magnetic Anomaly, Pinal County, Arizona

Regional aeromagnetic surveys passively measure the total magnetic intensity (TMI) and are a foundational tool used in mineral exploration (Airo, 2015). With the increased global demand and the number of critical mineral resources required for manufacturing high-tech devices, developing high-quality, regional-scale geophysical surveys could aid critical mineral exploration efforts and geologic mapping. In 2019, the U. S. Geological Survey launched the Earth Mapping Resources Initiative (Earth MRI) to modernize the geologic and geophysical mapping of regions that have the potential to contain critical mineral resources within the United States. In support of planning Earth MRI geophysical surveys, Drenth and Grauch (2019) defined five aeromagnetic data quality rankings (rank 1 through rank 5) applying them to the airborne geophysical survey inventory of the United States (Johnson et al., 2021). Rank 1 aeromagnetic surveys are of the highest quality, meeting modern standards and allowing best practices for qualitative and quantitative interpretation; whereas rank 5 aeromagnetic surveys are of the lowest quality, being useful only for qualitative interpretation of broad features. Through the Earth MRI effort, 48 high-quality, regional-scale rank 1 and 2 airborne magnetic and radiometric geophysical surveys have been planned, collected, or publicly release through May 2025 (U. S. Geological Survey, 2025). Here, a portion of a rank 1 Earth MRI aeromagnetic survey in southeast Arizona is presented and compared to a legacy rank 5 aeromagnetic survey over the Mammoth Magnetic Anomaly (MMA), demonstrating how modern, high-quality aeromagnetic data improves our view of crustal geology, aiding mineral exploration.

Arizona

Advancing sustainable groundwater management with a hydro-economic system model: Investigations in the Harney Basin, Oregon

Groundwater resources frequently trend toward unsustainable levels because, absent effective institutions, individual water users generally act independently without considering the impacts on other users. Hydro-economic models (HEMs) of human-natural systems can play a positive role toward successful groundwater management by yielding valuable knowledge and insight. The current study explores how an HEM that captures essential physical and economic characteristics of a system can shed light on the system's processes and dynamics to benefit stakeholders, managers, and also researchers. These propositions are illustrated using the Harney Basin, Oregon, which has seen large groundwater declines in the past 20 years. The HEM shows that: (a) although current groundwater pumping rates will gradually raise costs and reduce well yields, irrigators gain the highest aggregate economic return by continuing current pumping; (b) lowland areas of the basin are hydrologically connected, which limits the efficacy of remedies focused on regulations only in some portions of the basin; (c) community expectations regarding the efficacy of several proposed solutions are overly optimistic; and (d) the study's scenarios identify interventions that would stabilize the groundwater system and prevent additional adverse impacts on residential and livestock wells and groundwater-dependent ecosystems. These interventions would require limiting groundwater pumping by nearly half and reducing annual profits by $7.5–$9.0M. The HEM also demonstrated its value to researchers: its insights shifted attention toward questions about Oregon's existing groundwater institutions and their inability to adaptively manage the transition from abundant groundwater to scarce groundwater in a timely manner.

Oregon

Salinas Valley integrated hydrologic and reservoir operations models, Monterey and San Luis Obispo Counties, California

The area surrounding the Salinas Valley groundwater basin in Monterey and San Luis Obispo Counties of California is a highly productive agricultural area, contributes substantially to the local economy, and provides a substantial portion of vegetables and other agricultural commodities to the Nation. This region of California provides about half of the Nation’s lettuce, celery, broccoli, and spinach each year. Thus, this agricultural area provides substantial volumes of agricultural products not just for California but for the United States. Changes in population and increased agricultural development, which includes a shift toward more water-intensive crops, and climate variability, have put increasing demand on both surface-water and groundwater resources in the valley. This situation has resulted in water management challenges in the Salinas Valley that generally relate to the distribution of the water supply throughout the basin. Where and when the water is present in the surface and subsurface does not coincide with where and when the water is needed. Historically, to deal with the distribution issue, water has been used conjunctively in the valley. Conjunctive use is a water management strategy that coordinates surface-water and groundwater use to maximize water availability. Groundwater is used throughout the Salinas Valley to meet water demands when surface-water supplies are insufficient. The availability of surface water is constrained by climate. Precipitation and streamflow vary seasonally and year to year. Although there are two reservoirs in the Salinas Valley to capture and store water during wet periods, the only conveyance of reservoir water to coastal agricultural areas is the Salinas River. Increasing demand for groundwater and surface-water resources throughout the Salinas Valley has resulted in undesirable effects from unsustainable water use, such as surface-water depletion, groundwater-level declines, storage depletion in the principal aquifers, and seawater intrusion. To address these escalating issues, local communities, water management agencies, and groundwater sustainability agencies are evaluating how to sustainably manage both their surface-water and groundwater resources. To meet water demands and reduce the undesirable effects of unsustainable water use, continued conjunctive management of surface water and groundwater would ideally incorporate strategies to deal with increases in demand and climate variability. To evaluate the challenging water management issues in the Salinas Valley, the U.S. Geological Survey, Monterey County Water Resources Agency, and the Salinas Valley Basin Groundwater Sustainability Agency developed a comprehensive suite of models that represent the Salinas Valley hydrogeologic system called the Salinas Valley System Model. The geologic framework is known as the Salinas Valley Geologic Framework and was developed to characterize the subsurface using various topographic and geologic data sources, including information on hydrogeologic units, their surfaces and extents, geologic structures, lithology, and elevations from borehole data and cross sections, as well as details on faults and existing models. The surface-water model is called the Salinas Valley Watershed Model and simulates the Salinas River watershed. Monthly surface-water inflows into the integrated hydrologic model domain were simulated using the Salinas Valley Watershed Model. The historical model uses historical climate data, water and land use data, and reservoir releases to simulate agricultural operations, including landscape water demands, diversions, and reclaimed wastewater. The operational model adds an embedded reservoir operations framework to the simulation of the historical model that allows specified operational rules to simulate reservoir releases and changes in reservoir storage. The operational model assumes current reservoir operations and constant land use, which differs from historical conditions. Thus, the operational model is a hypothetical baseline model that can be used by local water managers to evaluate and quantify potential benefits of water supply projects. Together, the geologic framework, watershed, historical, and operational models form a tool that can be used to simulate irrigated agriculture and associated reservoir operations of the integrated hydrologic system of the Salinas Valley.

California

StreamStats—A quarter century of delivering web-based geospatial and hydrologic information to the public, and lessons learned

StreamStats is a U.S. Geological Survey (USGS) web application that provides streamflow statistics, such as the 1-percent annual exceedance probability peak flow, the mean flow, and the 7-day, 10-year low flow, to the public through a map-based user interface. These statistics are used in many ways, such as in the design of roads, bridges, and other structures; in delineation of floodplains for land-use zoning and setting of insurance rates; for regulatory purposes, such as the permitting of wastewater discharges; and for hydrologic and climate change studies. StreamStats was first developed for Massachusetts and released in 2001. The application provided users with the ability to obtain streamflow statistics computed from data collected at USGS streamgages and to obtain estimates of streamflow statistics for user-selected ungaged sites. Massachusetts StreamStats used geographic information system software and digital mapping to compute drainage-basin characteristics, which were then used in statistical models to estimate streamflow statistics for the user-selected sites. The statistical models were in the form of equations that were developed through a process known as regression analysis. StreamStats was the first known web application with the ability to do interactive geoprocessing. The utility of Massachusetts StreamStats was instantly apparent, leading the USGS to develop a version of StreamStats that could be implemented nationally. USGS State offices normally were required to develop custom regression equations and prepare local digital mapping data needed for implementing StreamStats for their States. Funding needed to complete this work usually was provided through cooperative agreements between the USGS and State agencies. In 2004, Idaho became the first to be released in the national version of StreamStats. By 2023, 44 States were fully implemented and six were undergoing implementation. StreamStats has undergone many modifications over the years to keep up with changes to the underlying software and to add functionality. Customized functionality and separate linked StreamStats applications were developed for several States. Meeting the high demand for additions and improvements to StreamStats while also adhering to budgetary constraints has, at times, been challenging. The StreamStats development team has identified numerous additional improvements that could be made to provide better performance and more functionality. The lessons learned from the experience of building and operating StreamStats for nearly 25 years could be relevant to others interested in pursuing efforts of a similar scale.

Circular

Temperature

The “National Field Manual for the Collection of Water-Quality Data” (NFM) provides guidelines and procedures for U.S. Geological Survey (USGS) personnel who collect data used to assess the quality of the Nation’s surface-water and groundwater resources. This chapter, NFM A6.1, provides guidance and protocols for the measurement of temperature of air, of a surface-water body or in groundwater, which include the scientific basis of the measurement, selection and maintenance of equipment, calibration verification, troubleshooting, and procedures for measurement and reporting. It updates and supersedes USGS Techniques of Water-Resources Investigations, book 9, chapter A6.1, version 2.0, by Franceska D. Wilde. Temperature of air and water is routinely measured when water samples are collected, is often measured continually at USGS streamgages, and is a parameter regularly measured during laboratory and field experiments. The field method for measuring temperature described in this chapter is applicable to air and most natural waters. Before 2017, the NFM chapters were released in the USGS Techniques of Water-Resources Investigations series. Effective in 2018, new and revised NFM chapters are being released in the USGS Techniques and Methods series; this series change does not affect the content and format of the NFM. More information is in the general introduction to the NFM (USGS Techniques and Methods, book 9, chapter A0) at https://doi.org/10.3133/tm9A0 . The authoritative current versions of NFM chapters are available in the USGS Publications Warehouse at https://pubs.usgs.gov/ . Comments, questions, and suggestions related to the NFM can be addressed to nfm@usgs.gov .

Techniques and Methods

Seismic techniques and suggested instrumentation to monitor volcanoes

Introduction Changes in the pressure or location of magma can stress or break surrounding rocks and trigger flow of nearby waters and gases, causing seismic signals, such as discrete earthquakes and tremor. These phenomena are types of seismic unrest that commonly precede eruption and can be used to forecast volcanic activity. Mass movements at the surface, including avalanches, debris flows, and lahars, may also generate seismic signals that are specifically addressed in chapter H , this volume (Thelen and others, 2024). Our focus in this chapter is to determine the levels of instrumentation recommended to produce high-quality, well-constrained seismic observations important for early warning of impending eruptions, detecting changes in ongoing eruptions, and characterizing other hazardous volcanic events. There are emerging techniques and new types of instrumentation, such as distributed acoustic sensing or rotational seismometers, that we do not consider here. These types of instrumentation show promise for monitoring but still require maturation before being considered more generally in volcano monitoring. Most of the capabilities mentioned below are universal for all types of volcanic systems, although some are best applied to stratovolcanoes with an apical single vent. In some settings, such as calderas or shield volcanoes, we must broaden coverage to include multiple possible storage regions or vent locations. As an example, Thelen (2014) discretized the long rift zones of shield volcanoes in Hawaiʻi as a set of evenly spaced “vents.” In this construct, each vent comes with recommendations, and several thousand network configurations were simulated to assess the effect on network quality levels and to determine the most efficient network design. The same process could be applied in a caldera setting or a volcanic field, where an evenly spaced grid of potential vents is considered. Localized recommendations for each unique system are beyond the scope of this report and left up to local experts to assess based on the conditions, restrictions, and requirements of each volcano.

Scientific Investigations Report

Technique for estimating depth of floods in Tennessee

Estimates of flood depths are needed for design of roadways across flood plains and for other types of construction along streams. Equations for estimating flood depths in Tennessee were derived using data for 150 gaging stations. The equations are based on drainage basin size and can be used to estimate depths of the 10-year and 100-year floods for four hydrologic areas. A method also was developed for estimating depth of floods having recurrence intervals between 10 and 100 years. Standard errors range from 22 to 30 percent for the 10-year depth equations and from 23 to 30 percent for the 100-year depth equations.

Tennessee

Hydrology and water and sediment quality at James Campbell National Wildlife Refuge near Kahuku, Island of Oahu, Hawaii

The James Campbell National Wildlife Refuge occupies two lowland marsh and pond complexes on the northern coastal plain of Oahu: the mostly natural ponds and wetlands of the Punamano Unit and the constructed ponds of the Kii Unit. The U.S. Fish and Wildlife Service manages the Refuge primarily to protect and enhance habitat for four endangered species of Hawaiian waterbirds. Kii Unit is fed by artesian wells and rainfall, whereas Punamano Unit is fed naturally by rainfall, runoff, and ground-water seepage. Streams drain from the uplands into lowland ditches that pass through Kii Unit on their way to the ocean. A high-capacity pump transfers water from the inner ditch terminus at Kii to the ocean outlet channel. Stormwaters also exit the inner ditch system over flood-relief swales near the outlet pump and through a culvert with a one-way valve. A hydrologic investigation was done from November 1996 through February 1998 to identify and quantify principal inflows and outflows of water to and from the Refuge, identify hydraulic factors affecting flooding, document ground-water/surface-water interactions, determine the adequacy of the current freshwater supply, and determine water and sediment quality. These goals were accomplished by installing and operating a network of stream-gaging stations, meteorology stations, and shallow ground-water piezometers, by computing water budgets for the two Refuge units, and by sampling and analyzing water and pond-bottom sediments for major ions, trace metals, and organic compounds. Streamflow during the study was dominated by winter stormflows, followed by a gradual recession of flow into summer 1997, as water that had been stored in alluvial fans drained to lowland ditches. Outflow at the ditch terminus in 1997 was 125 million gallons greater than measured inflow to the coastal plain, mainly reflecting gains from ground water along the ditches between outlying gages and the ditch terminus. Of the measured 1997 outflow, 98 percent was through the Kii outlet pump, with the outlet culvert valve only opening for brief periods during storms. Large volumes of stormflow overflowed the flood-relief swales unmeasured. The largest storm of the study, in November 1996, was estimated to have a flood frequency of about 3 to 4 years. Streamflow exceeded culvert capacity and overtopped Kamehameha Highway at Kalaeokahipa Stream and Hospital ditch. Slight overbank flooding in Kii ditch resulted strictly from high discharge. Minor overbank flooding farther out on the coastal plain probably was caused mainly by the small hydraulic gradients available to convey stormflows along the lowland ditches. Stormwaters flooded Kii ponds and flowed back upstream along Punamano ditch into Punamano marsh, introducing suspended sediment and possibly other contaminants to the Refuge. Two smaller storms in January 1997 resulted in smaller flows and no overbank flooding. The Kii outlet pump ran continuously for 7 days during the November 1996 storm and for 1 to 2 days during the January 1997 storms. During all three storms, the outlet culvert valve opened and the inner ditches overtopped the flood-relief swales, allowing free outflow of water from the inner ditch. Backwater effects hindered drainage during the January 1997 storms at Hospital ditch at Kamehameha Highway, and at Punamano ditch at Nudist Camp Road (where the backflow into Punamano marsh in November 1996 constituted an extreme backwater effect). A probable marine backwater effect was imposed at the ocean outlet ditch during the November 1996 storm through a combination of high spring tides and wave setup from large surf. Whether this backwater effect propagated upstream in the ditches to affect inland sites could not be determined conclusively. A sand plug may have built up in the ocean outlet channel before the November 1996 storm, but if so, it probably washed out prior to, or early in the storm, and was not present at the time of peak stage at inland sites. A season-long buildup of the sand plug in late 1997 was inferred from rising water levels in the outlet ditch. Seawater flows up the outlet channel or over the sand berm and into the outer ditch system on most high tides, and particularly during spring high tides. Ponds and ditches of the Refuge and surrounding lowlands have mud- and clay-lined bottoms that form an effective confining unit and inhibit interaction with an underlying shallow limestone aquifer. At Kii Unit, pond levels are higher than adjacent ditch levels and underlying ground-water levels, establishing lateral and downward head gradients that could foster seepage losses from the ponds. Regional ground-water discharge from the Koolau aquifer to the coastal-plain sediments is mostly diffuse, but is concentrated where ridges of Koolau Basalt plunge beneath coastal-plain sediments near Punamano Unit and at the head of Hospital ditch. Kii ditch gains brackish ground water downstream of Kamehameha Highway. Wastewater disposal from the sewage treatment plant adjoining Kii Unit poses little or no threat to Refuge habitat. Disposal is at six injection wells located 0.45 mi away at Kahuku, and the wells inject into confined limestone aquifers that do not extend to Kii Unit. The natural freshwater supply to Punamano Unit is adequate for maintaining the wildlife habitat, judging from stable pond levels and low salinities there. A monthly water budget for Punamano showed an apparent annual deficit in measurable flows in 1997, requiring unmeasured ground-water gains equalling 51 inches of water. The freshwater supply to Kii Unit is inadequate according to Refuge managers, because there is not enough water to manipulate levels adequately in the ponds during most of the year, and particularly during the driest months. This is confirmed by monthly deficits in the water budget for the Kii ponds during summer months. However, the Kii budget showed an annual surplus in measurable flows for 1997 equalling 24 inches of water. Unmeasured losses are required to explain the apparent annual surplus, such as discharge to the ditches through pond water-control structures and downward and/or lateral ground-water seepage. The apparent surplus at Kii is strictly hydrologic and is not a surplus in a management sense; it cannot be stored or used to supply the Refuge, but instead reflects losses from the system that render this amount of water unavailable for use. The budget year, 1997, was drier than normal (24 percent below long-term mean rainfall) and so the measured potential evaporation for 1997 was probably higher than the long-term mean. Few metals or organic compounds of potential concern were detected in pond and ditch waters and in pond-bottom sediments. Detected pesticides were at trace levels or just above minimum reporting limits. Exceptions that exceeded quality guidelines for freshwater sediment were copper and zinc in sediment from Kii ponds C and D, and copper in sediment from Punamano north pond. Therefore, urban and agricultural runoff probably have contributed little in the way of harmful metals or organic compounds to the Refuge, although the potential for such contribution remains from periodic flooding of the ponds by ditch stormflows. Salinity was low throughout most Refuge waters, qualifying as fresh to slightly brackish and suitable for the environmental needs of Refuge fauna. Higher salinities have been observed in ditches during past periods of sugarcane cultivation and saltwater aquaculture, however. Resumption of saltwater aquaculture could raise ditch salinities if saltwater effluents are disposed directly into the ditches, as they were in the past.

Hawaii

Transcriptional changes in wild Yukon River Chinook Salmon associated with Ichthyophonus infections

Objective We compared differentially expressed genes in Chinook Salmon Oncorhynchus tshawytscha with three divergent Ichthyophonus statuses (undetected, subclinical infections, or clinical disease; n = 100) to investigate associated transcriptomic responses. Disease associated with the fish parasite Ichthyophonus sp. was first diagnosed in adult Chinook Salmon from the Yukon River in the late 1980s and has subsequently been implicated in premature host mortality. Methods Chinook Salmon tissue sample collections and Ichthyophonus infection data were leveraged from a multi-agency collaboration during summer 2022 at three locations along the main-stem Yukon River that spanned nearly 2,000 km of freshwater migration (lower, middle, and upper river). We sequenced the transcriptome and compared this to infection status based on routine diagnostic procedures. Results Among the 17,569 genes for which messenger RNA was detected, we identified a transcription signature in the skeletal muscle that was associated with Ichthyophonus infections and included 53 differentially expressed genes. The differentially expressed genes and their pathways included those known for involvement in immune functions, energy synthesis, cellular breakdown, and reproduction—all processes that are known to be influenced by senescence during spawning migrations. Conclusions Results demonstrate a clear transcriptional difference between diseased fish (clinical disease group) and those in which Ichthyophonus was undetected, including identifying candidate markers for infection in this population. These results provide a foundation for development of nonlethal biomarkers to evaluate potential Ichthyophonus infections in Chinook Salmon based on gene transcription, protein products, or gene variants (e.g., polymorphisms).

Journal of Aquatic Animal Health

The distribution of bromide in water in the Floridan aquifer system, Duval County, northeastern Florida

Although Duval County, Florida, has ample ground-water resources for public supply, the potential exists for a problem with excessive disinfectant by-products. These disinfectant by-products result from the treatment of raw water containing low concentrations of bromide and naturally occurring organic compounds. Because of this potential problem, the relation of bromide concentrations to aquifer tapped, well location and depth, and chemical characteristics of water in the Floridan aquifer system underlying Duval County were studied to determine if these relations could be applied to delineate water with low-bromide concentrations for future supplies. In 1992, water samples from 106 wells that tap the Floridan aquifer system were analyzed for bromide and major dissolved constituents. A comparison of bromide concentrations from the 1992 sampling with data from earlier studies (1979-80) indicates that higher bromide concentrations were detected during the earlier studies. The difference between the old and new data is probably because of a change in analytical methodology in the analysis of samples. Bromide concentrations exceeded the detection limit (0.10 milligrams per liter) in water from 28 of the 106 wells (26 percent) sampled in 1992. The maximum concentration was 0.56 milligrams per liter. There were no relations between bromide and major dissolved constituents, well depth, or aquifer tapped that would be useful for determining bromide concentrations. Areal patterns of bromide occurrence are not clearly defined, but areas with relatively high bromide concentrations tend to be located in a triangular area near the community of Sunbeam, Florida, and along the St. Johns River throughout Duval County.

Florida

Probabilistic assessment of postfire debris-flow inundation in response to forecast rainfall

Communities downstream of burned steep lands face increases in debris-flow hazards due to fire effects on soil and vegetation. Rapid postfire hazard assessments have traditionally focused on quantifying spatial variations in debris-flow likelihood and volume in response to design rainstorms. However, a methodology that provides estimates of debris-flow inundation downstream of burned areas based on forecast rainfall would provide decision-makers with information that directly addresses the potential for downstream impacts. We introduce a framework that integrates a 24 h lead-time ensemble precipitation forecast with debris-flow likelihood, volume, and runout models to produce probabilistic maps of debris-flow inundation. We applied this framework to simulate debris-flow inundation associated with the 9 January 2018 debris-flow event in Montecito, California, USA. When the observed debris-flow volumes were used to drive the probabilistic forecast model, analysis of the simulated inundation probabilities demonstrates that the model is both reliable and sharp. In the fully predictive model, however, in which debris-flow likelihood and volume were computed from the atmospheric model ensemble's predictions of peak 15 min rainfall intensity, I 15 , the model generally under-forecasted the inundation area. The observed peak I 15 lies in the upper tail of the atmospheric model ensemble spread; thus a large fraction of ensemble members forecast lower I 15 than observed. Using these I 15 values as input to the inundation model resulted in lower-than-observed flow volumes which translated into under-forecasting of the inundation area. Even so, approximately 94 % of the observed inundated area was forecast to have an inundation probability greater than 1 %, demonstrating that the observed extent of inundation was generally captured within the range of outcomes predicted by the model. Sensitivity analyses indicate that debris-flow volume and two parameters associated with debris-flow mobility exert significant influence on inundation predictions, but reducing uncertainty in postfire debris-flow volume predictions will have the largest impact on reducing inundation outcome uncertainty. This study represents a first step toward a near-real-time hazard assessment product that includes probabilistic estimates of debris-flow inundation and provides guidance for future improvements to this and similar model frameworks by identifying key sources of uncertainty.

California