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At least 685 records · Page 38Linked to original sources

USGS2016

Potential of environmental DNA to evaluate Northern pike ( Esox lucius ) eradication efforts: An experimental test and case study

Determining the success of invasive species eradication efforts is challenging because populations at very low abundance are difficult to detect. Environmental DNA (eDNA) sampling has recently emerged as a powerful tool for detecting rare aquatic animals; however, detectable fragments of DNA can persist over time despite absence of the targeted taxa and can therefore complicate eDNA sampling after an eradication event. This complication is a large concern for fish eradication efforts in lakes since killed fish can sink to the bottom and slowly decay. DNA released from these carcasses may remain detectable for long periods. Here, we evaluated the efficacy of eDNA sampling to detect invasive Northern pike ( Esox lucius ) following piscicide eradication efforts in southcentral Alaskan lakes. We used field observations and experiments to test the sensitivity of our Northern pike eDNA assay and to evaluate the persistence of detectable DNA emitted from Northern pike carcasses. We then used eDNA sampling and traditional sampling (i.e., gillnets) to test for presence of Northern pike in four lakes subjected to a piscicide-treatment designed to eradicate this species. We found that our assay could detect an abundant, free-roaming population of Northern pike and could also detect low-densities of Northern pike held in cages. For these caged Northern pike, probability of detection decreased with distance from the cage. We then stocked three lakes with Northern pike carcasses and collected eDNA samples 7, 35 and 70 days post-stocking. We detected DNA at 7 and 35 days, but not at 70 days. Finally, we collected eDNA samples ~ 230 days after four lakes were subjected to piscicide-treatments and detected Northern pike DNA in 3 of 179 samples, with a single detection at each of three lakes, though we did not catch any Northern pike in gillnets. Taken together, we found that eDNA can help to inform eradication efforts if used in conjunction with multiple lines of inquiry and sampling is delayed long enough to allow full degradation of DNA in the water.

PLoS ONE↗

USGS2022

Testing the potential of streamflow data to predict spring migration of an ungulate herds

In mountainous and high latitude regions, migratory animals exploit green waves of emerging vegetation coinciding with rising daily mean temperatures initiating snowmelt across the landscape. Snowmelt also causes rivers and streams draining these regions to swell, a process referred to as to as the ‘spring pulse.’ Networks of streamgages measuring streamflow in these regions often have long-term and continuous periods of record available in real-time and at the daily time step, and thus produce data with potential to predict temporal migration patterns for species exploiting green waves. We tested the potential of models informed by streamflow data to predict timing of spring migration of mule deer ( Odocoileus hemionus ) herds in a headwater basin of the Colorado River. Models using streamflow data were compared with those informed by traditional temperature-derived measures of the onset of spring. Non-parametric linear-regression techniques were used to test for temporal stationarity in each variable, and logistic-regression models were used to produce probabilities of migration initiation. Our analysis indicates that models using daily streamflow data can perform as well as those using temperature-derived data to predict past-migration patterns, and nearly as well in potential to forecast future migrations. The best performing model was used to generate probabilities of onset of migration for mule deer herds over the 69-year period-of-record from a streamgage. That model indicated spring migration has been trending toward earlier initiations, with modeled median initiations shifting from a Julian day of 123 in the mid 20 th century to Julian day 115 over the most recent two decades. The period of 1960 to 1979 had the latest modeled median initiations with Julian day of 128. The analyses demonstrate promise for merging existing hydrologic and biological data collection platforms in these regions to explore timing of past migration patterns and predict migration onsets in real-time.

Colorado, Wyoming↗

USGS2022

Diagnostic testing of chronic wasting disease in white-tailed deer (Odocoileus virginianus) by RT-QuIC using multiple tissues

Chronic wasting disease (CWD) is a fatal prion disease affecting cervids (deer, elk, moose). Current methods to monitor individual disease state include highly invasive antemortem rectal biopsy or postmortem brain biopsy. Efficient, sensitive, and selective antemortem and postmortem testing of populations would increase knowledge of the dynamics of CWD epizootics as well as provide a means to track CWD progression into previously unaffected areas. Here, we analyzed the presence of CWD prions in skin samples from two easily accessed locations (ear and belly) from 30 deceased white-tailed deer ( Odocoileus viginianus ). The skin samples were enzymatically digested and analyzed by real-time quaking-induced conversion (RT-QuIC). The diagnostic sensitivity of the ear and belly skin samples were both 95%, and the diagnostic specificity of the ear and belly skin were both 100%. Additionally, the location of the skin biopsy on the ear does not affect specificity or sensitivity. These results demonstrate the efficacy of CWD diagnosis with skin biopsies using RT-QuIC. This method could be useful for large scale antemortem population testing.

Wisconsin↗

USGS1982

Subacute toxicity testing with young birds: Response in relation to age and intertest variability of LC50 estimates

The variability in toxic response of Japanese quail (Coturnix coturnix japonica) to a standardized 5-day subacute feeding trial was studied while age was increased at weekly intervals from 1 to 21 days and at different times with 14-day-old birds. The objectives were to identify the strengths and limitations of this subacute toxicity protocol and to provide possible explanations for differences in response among the various classes of pesticides. The variables included the median lethal concentration (LC50), the dose-response slope, food consumption, mortality patterns, and procedures of preparing toxic diets. Carbamate, organophosphate, chlorinated hydrocarbon, and organic mercury compounds were represented. A total of 60 subacute tests were involved in this evaluation. The general conclusions of the study are as follows: (1) The basic protocol yields good within-laboratory reproducibility of results. (2) The LC50s increase as birds grow older, and the change was reasonably predictable between 7 and 21 days of age. (3) Monitoring of time-related response patterns, such as food consumption, onset and remission of overt toxicity, and mortality, is critical to the evaluation of potential hazard. (4) Different orders of toxicity may occur at different ages for an array of compounds, but differences do not appear to be large enough to seriously alter the interpretation. (5) For optimal comparisons, all testing should be conducted on birds of a single age.

Book chapter↗

USGS1999

Testing and extension of a sea lamprey feeding model

A previous model of feeding by sea lamprey Petromyzon marinus predicted energy intake and growth by lampreys as a function of lamprey size, host size, and duration of feeding attachments, but it was applicable only to lampreys feeding at 10°C and it was tested against only a single small data set of limited scope. We extended the model to other temperatures and tested it against an extensive data set (more than 700 feeding bouts) accumulated during experiments with captive sea lampreys. Model predictions of instantaneous growth were highly correlated with observed growth, and a partitioning of mean squared error between model predictions and observed results showed that 88.5% of the variance was due to random variation rather than to systematic errors. However, deviations between observed and predicted values varied substantially, especially for short feeding bouts. Predicted and observed growth trajectories of individual lampreys during multiple feeding bouts during the summer tended to correspond closely, but predicted growth was generally much higher than observed growth late in the year. This suggests the possibility that large overwintering lampreys reduce their feeding rates while attached to hosts. Seasonal or size-related shifts in the fate of consumed energy may provide an alternative explanation. The lamprey feeding model offers great flexibility in assessing growth of captive lampreys within various experimental protocols (e.g., different host species or thermal regimes) because it controls for individual differences in feeding history.

Transactions of the American Fisheries Society↗

USGS1997

Differences in detection of Aeromonas salmonicida in covertly infected salmonid fishes by the stress-inducible furunculosis test and culture-based assays

Accurate detection of Aeromonas salmonicida subsp. salmonicida (the cause of furunculosis disease) in covertly infected salmonids is difficult and is a cause of concern for those involved in fish health inspection and resource management programs. In this study, we examined populations of brook trout Salvelinus fontinalis , Atlantic salmon Salmo salar , and lake trout Salvelinus namaycush that previously sustained natural episodes of furunculosis. Consequently, the sampled fish were presumed to harbor latent infections. Mucus, gill, liver, kidney, heart, spleen, and intestine samples ( N = 100 fish per group sampled) were processed and examined by (1) direct dilution counts and (2) quadrant streaking after a 48‐h pre‐enrichment in trypticase soy broth (TSB). Another subsample of fish from each group was then subjected to stress‐inducible furunculosis tests. Stress tests detected A. salmonicida in three of four groups of fish that were examined whereas the pathogen was detected in only two of the groups analyzed with culture‐based assays. Although pre‐enrichment in TSB enhanced detection within internal sampling sites including the liver, heart, spleen, and kidney, enrichment did not enhance detection from mucus, gill, or intestinal samples.

Journal of Aquatic Animal Health↗

USGS2002

The 1999 Mw 7.1 Hector Mine, California, earthquake: A test of the stress shadow hypothesis?

We test the stress shadow hypothesis for large earthquake interactions by examining the relationship between two large earthquakes that occurred in the Mojave Desert of southern California, the 1992 M w 7.3 Landers and 1999 M w 7.1 Hector Mine earthquakes. We want to determine if the 1999 Hector Mine earthquake occurred at a location where the Coulomb stress was increased (earthquake advance, stress trigger) or decreased (earthquake delay, stress shadow) by the previous large earthquake. Using four models of the Landers rupture and a range of possible hypocentral planes for the Hector Mine earthquake, we discover that most scenarios yield a Landers-induced relaxation (stress shadow) on the Hector Mine hypocentral plane. Although this result would seem to weigh against the stress shadow hypothesis, the results become considerably more uncertain when the effects of a nearby Landers aftershock, the 1992 M L 5.4 Pisgah earthquake, are taken into account. We calculate the combined static Coulomb stress changes due to the Landers and Pisgah earthquakes to range from -0.3 to +0.3 MPa (- 3 to +3 bars) at the possible Hector Mine hypocenters, depending on choice of rupture model and hypocenter. These varied results imply that the Hector Mine earthquake does not provide a good test of the stress shadow hypothesis for large earthquake interactions. We use a simple approach, that of static dislocations in an elastic half-space, yet we still obtain a wide range of both negative and positive Coulomb stress changes. Our findings serve as a caution that more complex models purporting to explain the triggering or shadowing relationship between the 1992 Landers and 1999 Hector Mine earthquakes need to also consider the parametric and geometric uncertainties raised here.

California↗

USGS2008

Testing small-aperture array analysis on well-located earthquakes, and application to the location of deep tremor

We have here analyzed local and regional earthquakes using array techniques with the double aim of quantifying the errors associated with the estimation of propagation parameters of seismic signals and testing the suitability of a probabilistic location method for the analysis of nonimpulsive signals. We have applied the zero-lag cross-correlation method to earthquakes recorded by three dense arrays in Puget Sound and Vancouver Island to estimate the slowness and back azimuth of direct P waves and S waves. The results are compared with the slowness and back azimuth computed from the source location obtained by the analysis of data recorded by the Pacific Northwest seismic network (PNSN). This comparison has allowed a quantification of the errors associated with the estimation of slowness and back azimuth obtained through the analysis of array data. The statistical analysis gives ??BP = 10?? and ??BS = 8?? as standard deviations for the back azimuth and ??SP = 0.021 sec/km and ??SS = 0.033 sec /km for the slowness results of the P and S phases, respectively. These values are consistent with the theoretical relationship between slowness and back azimuth and their uncertainties. We have tested a probabilistic source location method on the local earthquakes based on the use of the slowness estimated for two or three arrays without taking into account travel-time information. Then we applied the probabilistic method to the deep, nonvolcanic tremor recorded by the arrays during July 2004. The results of the tremor location using the probabilistic method are in good agreement with those obtained by other techniques. The wide depth range, of between 10 and 70 km, and the source migration with time are evident in our results. The method is useful for locating the source of signals characterized by the absence of pickable seismic phases.

Bulletin of the Seismological Society of America↗

USGS2018

Laboratory tests of three Z‐Land Fairfield Nodal 5‐Hz, three‐component sensors

We conduct a number of laboratory tests at the Albuquerque Seismological Laboratory to verify the self‐noise and fidelity in which 3 three‐component Fairfield Nodal Z‐Land, Generation 2, 5‐Hz sensors are able to record seismic signals. In addition to the incoherent self‐noise of the sensors, we estimate the sensitivity of the units in digital volts/m/s, the damping, and the free period. These three parameters allow us to completely characterize the response of the instruments. We find that the responses of all components match a mean‐derived response to within 5% of amplitude and 0.03 radians in phase. This close agreement suggests that for most applications a nominal response is suitable. We also checked the timing of the units as compared to a Quanterra Q330HR and found good agreement up to 200samples/s . Finally, we compared the results of our noise tests on these sensors to a couple of nodal sensors recently deployed at the Community Wavefield Demonstration Experiment in north‐central Oklahoma and found that local site noise and not the sensor self‐noise is a fundamental limiter in the resolution of these deployed sensors at frequencies above ∼0.1Hz .

Seismological Research Letters↗

USGS2022

Testing the ShakeAlert earthquake early warning system using synthesized earthquake sequences

We test the behavior of the United States (US) West Coast ShakeAlert earthquake early warning (EEW) system during temporally close earthquake pairs to understand current performance and limitations. We consider performance metrics based on source parameter and ground‐motion forecast accuracy, as well as on alerting timeliness. We generate ground‐motion times series for synthesized earthquake sequences from real data by combining the signals from pairs of well‐recorded earthquakes (⁠4.4≤M≤7.1⁠) using time shifts ranging from −60 to +180 s. We examine fore‐ and aftershock sequences, near‐simultaneous events in different source regions, and simulated out‐of‐network and offshore earthquakes. We find that the operational ShakeAlert algorithms Earthquake Point‐source Integrated Code (EPIC) and Finite‐Fault Rupture Detector (FinDer) and the Propagation of Local Undamped Motion (PLUM) method perform largely as expected: EPIC provides the best source location estimates and is often fastest but can underestimate magnitudes or, in extreme cases, miss large earthquakes; FinDer provides real‐time line‐source models and unsaturated magnitude estimates for large earthquakes but currently cannot process concurrent events and may mislocate offshore earthquakes; PLUM identifies pockets of strong ground motion, but can overestimate alert areas. Implications for system performance are: (1) spatially and temporally close events are difficult to identify separately; (2) challenging scenarios with foreshocks that are close in space and time can lead to missed alerts for large earthquakes; and (3) in these situations the algorithms can often estimate ground motion better than source parameters. To improve EEW, our work suggests revisiting the current algorithm weighting in ShakeAlert, to continue developments that focus on using ground‐motion data to aggregate alerts from multiple algorithms, and to investigate methods to optimally leverage algorithm ground‐motion estimates. For testing and certification of EEW performance in ShakeAlert and other EEW systems where applicable, we also suggest that 25 of our 73 scenarios become part of the baseline data set.

California↗

USGS2010

Are wolves saving Yellowstone's aspen? A landscape-level test of a behaviorally mediated trophic cascade

Behaviorally mediated trophic cascades (BMTCs) occur when the fear of predation among herbivores enhances plant productivity. Based primarily on systems involving small-bodied predators, BMTCs have been proposed as both strong and ubiquitous in natural ecosystems. Recently, however, synthetic work has suggested that the existence of BMTCs may be mediated by predator hunting mode, whereby passive (sit-and-wait) predators have much stronger effects than active (coursing) predators. One BMTC that has been proposed for a wide-ranging active predator system involves the reintroduction of wolves ( Canis lupus ) to Yellowstone National Park, USA, which is thought to be leading to a recovery of trembling aspen ( Populus tremuloides ) by causing elk ( Cervus elaphus ) to avoid foraging in risky areas. Although this BMTC has been generally accepted and highly popularized, it has never been adequately tested. We assessed whether wolves influence aspen by obtaining detailed demographic data on aspen stands using tree rings and by monitoring browsing levels in experimental elk exclosures arrayed across a gradient of predation risk for three years. Our study demonstrates that the historical failure of aspen to regenerate varied widely among stands (last recruitment year ranged from 1892 to 1956), and our data do not indicate an abrupt cessation of recruitment. This pattern of recruitment failure appears more consistent with a gradual increase in elk numbers rather than a rapid behavioral shift in elk foraging following wolf extirpation. In addition, our estimates of relative survivorship of young browsable aspen indicate that aspen are not currently recovering in Yellowstone, even in the presence of a large wolf population. Finally, in an experimental test of the BMTC hypothesis we found that the impacts of elk browsing on aspen demography are not diminished in sites where elk are at higher risk of predation by wolves. These findings suggest the need to further evaluate how trophic cascades are mediated by predator–prey life history and ecological context.

Yellowstone↗

USGS2010

Soil-test N recommendations augmented with PEST-optimized RZWQM simulations

Improved understanding of year-to-year late-spring soil nitrate test (LSNT) variability could help make it more attractive to producers. We test the ability of the Root Zone Water Quality Model (RZWQM) to simulate watershed-scale variability due to the LSNT, and we use the optimized model to simulate long-term field N dynamics under related conditions. Autoregressive techniques and the automatic parameter calibration program PEST were used to show that RZWQM simulates significantly lower nitrate concentration in discharge from LSNT treatments compared with areas receiving fall N fertilizer applications within the tile-drained Walnut Creek, Iowa, watershed (>5 mg N L-1 difference for the third year of the treatment, 1999). This result is similar to field-measured data from a paired watershed experiment. A statistical model we developed using RZWQM simulations from 1970 to 2005 shows that early-season precipitation and early-season temperature account for 90% of the interannual variation in LSNT-based fertilizer N rates. Long-term simulations with similar average N application rates for corn (Zea mays L.) (151 kg N ha-1) show annual average N loss in tile flow of 20.4, 22.2, and 27.3 kg N ha -1 for LSNT, single spring, and single fall N applications. These results suggest that (i) RZWQM is a promising tool to accurately estimate the water quality effects of LSNT; (ii) the majority of N loss difference between LSNT and fall applications is because more N remains in the root zone for crop uptake; and (iii) year-to-year LSNT-based N rate differences are mainly due to variation in early-season precipitation and temperature. Copyright ?? 2010 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. All rights reserved.

Journal of Environmental Quality↗

USGS2005

Verification of sex from harvested sea otters using DNA testing

We used molecular genetic methods to determine the sex of 138 sea otters ( Enhydra lutris ) harvested from 3 regions of Alaska from 1994 to 1997, to assess the accuracy of post‐harvest field‐sexing. We also tested each of a series of factors associated with errors in field‐sexing of sea otters, including male or female bias, age‐class bias, regional bias, and bias associated with hunt characteristics. Blind control results indicated that sex was determined with 100% accuracy using polymerase chain reaction (PCR) amplification using primers that co‐amplify the zinc finger‐Y‐X gene, located on both the mammalian Y‐ and X‐chromosomes, and Testes Determining Factor (TDF), located on the mammalian Y‐chromosome. DNA‐based sexing revealed that 12.3% of the harvested sea otters were incorrectly sexed in the field, with most errors (13 of 17) occurring as males incorrectly reported as females. Thus, female harvest was overestimated. Using logistic regression analysis, we detected no statistical association of incorrect determination of sex in the field with age class, hunt region, or hunt type. The error in field‐sexing appears to be random, at least with respect to the variables evaluated in this study.

Wildlife Society Bulletin↗

USGS1988

A field test for differences in condition among trapped and shot mallards

We tested predictions from the condition bias hypothesis (Weatherland and Greenwood 1981) regarding the effects of sampling methods of body weights of mallards ( Anas platyrhynchos ) at White River National Wildlife Refuge (WRNWR), Arkansas, during 24 November-8 December 1985. Body weights of 84 mallards caught with unbaited rocket nets in a natural wetland were used as experimental controls and compared to the body weights of 70 mallards captured with baited rocket nets, 86 mallards captured with baited swim-in traps, and 130 mallards killed by hunters. We found no differences (P > 0.27) in body weight among sampling methods, but body condition (wt/wing length) of the birds killed by hunters was less (P < 0.02) than that of the controls. Power of the test statistics to reject the null hypothesis of equal body weights among sampling methods was > 0.75 for differences > 50 g. The condition bias hypothesis probably applies to ducks killed by hunters but not to trapping operations when substantial (> 20 at 1 time) numbers of birds are captured.

Arkansas↗

USGS1989

Generalized procedures for testing hypotheses about survival or recovery rates

Comparisons of survival or recovery rates from different time periods or geographic regions may be difficult to accomplish using the Z-tests suggested by Brownie et al. (1985). We propose a general Chi-square statistic that addresses an unambiguous null hypothesis of homogeneity among several survival or recovery rates. With this statistic, specific hypotheses of differences in rates can be simultaneously tested using contrasts. If necessary, a posteriori multiple comparisons can also be conducted that incorporate an adjustment for Type I error.

Journal of Wildlife Management↗

USGS2000

Testing assumptions for unbiased estimation of survival of radiomarked harlequin ducks

Unbiased estimates of survival based on individuals outfitted with radiotransmitters require meeting the assumptions that radios do not affect survival, and animals for which the radio signal is lost have the same survival probability as those for which fate is known. In most survival studies, researchers have made these assumptions without testing their validity. We tested these assumptions by comparing interannual recapture rates (and, by inference, survival) between radioed and unradioed adult female harlequin ducks ( Histrionicus histrionicus ), and for radioed females, between right-censored birds (i.e., those for which the radio signal was lost during the telemetry monitoring period) and birds with known fates. We found that recapture rates of birds equipped with implanted radiotransmitters (21.6 ± 3.0%; x̄ ± SE) were similar to unradioed birds (21.7 ± 8.6%), suggesting that radios did not affect survival. Recapture rates also were similar between right-censored (20.6 ± 5.1%) and known-fate individuals (22.1 ± 3.8%), suggesting that missing birds were not subject to differential mortality. We also determined that capture and handling resulted in short-term loss of body mass for both radioed and unradioed females and that this effect was more pronounced for radioed birds (the difference between groups was 15.4 ± 7.1 g). However, no difference existed in body mass after recapture 1 year later. Our study suggests that implanted radios are an unbiased method for estimating survival of harlequin ducks and likely other species under similar circumstances.

Alaska↗

USGS1990

Field testing of commercially manufactured capture collars on white-tailed deer

We conducted 31 tests of commercially manufactured capture collars on female white-tailed deer ( Odocoileus virginianus ) in the Superior National Forest, Minnesota, under temperatures from -37C to 22C. Deer were recaptured in 28 of the 31 tests; in the 3 failures, we remotely released the collars from the deer. Communication with the collars was achieved from up to 3.0 km on the ground and 26.5 km from the air.

Minnesota↗

USGS1984

Avian models for toxicity testing

The use of birds as test models in experimental and environmental toxicology as related to health effects is reviewed, and an overview of descriptive tests routinely used in wildlife toxicology is provided. Toxicologic research on birds may be applicable to human health both directly by their use as models for mechanistic and descriptive studies and indirectly as monitors of environmental quality. Topics include the use of birds as models for study of teratogenesis and embryotoxicity, neurotoxicity, behavior, trends of environmental pollution, and for use in predictive wildlife toxicology. Uses of domestic and wild-captured birds are discussed.

Journal of the American College of Toxicology↗