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Multinomial N-mixture models improve the applicability of electrofishing for developing population estimates of stream-dwelling Smallmouth Bass

Failure to account for variable detection across survey conditions constrains progressive stream ecology and can lead to erroneous stream fish management and conservation decisions. In addition to variable detection’s confounding long-term stream fish population trends, reliable abundance estimates across a wide range of survey conditions are fundamental to establishing species–environment relationships. Despite major advancements in accounting for variable detection when surveying animal populations, these approaches remain largely ignored by stream fish scientists, and CPUE remains the most common metric used by researchers and managers. One notable advancement for addressing the challenges of variable detection is the multinomial N -mixture model. Multinomial N -mixture models use a flexible hierarchical framework to model the detection process across sites as a function of covariates; they also accommodate common fisheries survey methods, such as removal and capture–recapture. Effective monitoring of stream-dwelling Smallmouth Bass Micropterus dolomieu populations has long been challenging; therefore, our objective was to examine the use of multinomial N -mixture models to improve the applicability of electrofishing for estimating absolute abundance. We sampled Smallmouth Bass populations by using tow-barge electrofishing across a range of environmental conditions in streams of the Ozark Highlands ecoregion. Using an information-theoretic approach, we identified effort, water clarity, wetted channel width, and water depth as covariates that were related to variable Smallmouth Bass electrofishing detection. Smallmouth Bass abundance estimates derived from our top model consistently agreed with baseline estimates obtained via snorkel surveys. Additionally, confidence intervals from the multinomial N -mixture models were consistently more precise than those of unbiased Petersen capture–recapture estimates due to the dependency among data sets in the hierarchical framework. We demonstrate the application of this contemporary population estimation method to address a longstanding stream fish management issue. We also detail the advantages and trade-offs of hierarchical population estimation methods relative to CPUE and estimation methods that model each site separately.

Missouri, Oklahoma↗

A history of human impacts on the Lake Erie fish community

The fisheries scientist working in the island region of Lake Erie has access to an extremely large and diverse freshwater fish community. It is the intention of this essay to discuss briefly that community and the impacts of human activities to provide future students and researchers with both current and historical information. Human settlements and development within the basin are discussed, followed by a description of the major stresses on the community, the impacts of those stresses, and the resulting or present-day fish community.

Book chapter↗

Introduction [to Issue 3]

Artificial propagation of aquatic organisms is increasing globally and currently accounts for approximately 32% of total world fishery production (Vannuccini, 2004). Between 1970 and 2000, aquaculture production of salmonids has grown from less than 200,000 metric tons per year to over 1.5 million metric tons (Tacon, 2003). In 1995, the number of Atlantic salmon (Salmo salar) far exceeded the carrying capacity of salmon in the wild but over 94% of all adult Atlantic salmon were in aquaculture environments (Gross, 1998). Since the 1970’s, concerns have arisen about interactions of hatchery and wild produced salmonids in native habitats. In response, research has addressed concerns about domestication and genetic impacts (Reisenbichler and McIntyre, 1977; Hindar et al., 1991; Waples, 1991; Clifford et al., 1998a; Fleming et al., 2000, 2002; Hard et al., 2000), transfer of disease and parasites (Johnsen and Jensen, 1994; Bakke and Harris, 1998), behavior of spawning adults (Fleming and Gross, 1992; Fleming et al., 1994; Økland et al., 1995; Youngston et al., 1998), differences in fitness traits and life history (Jonsson et al., 1991; Thodesen et al., 1999; McGinnity et al., 2003), and behavioral interactions between hatchery and wild juvenile salmon (Swain and Riddell, 1990; Johnsson et al., 1996; Clifford et al., 1998b). Much of this research has been driven by concerns about the impacts of escaped farmed Atlantic salmon in the North Atlantic Ocean (Hansen et al., 1991). Studies of the interactions between hatchery and wild salmonids, with few exceptions (see Myers et al., 2000), have primarily focused on interactions in freshwater environments. Interactions between hatchery and wild fishes in estuarine and marine environments have not received the same attention, but may have signifi- cant impacts on wild populations. To address this issue, we organized a symposium held at the 2003 annual meeting of the Western Division of the American Fisheries Society in San Diego, California. The session was titled Interactions of Hatchery and Wild Fishes in Marine and Estuarine Environments and included nine presentations. All but one presentation focused on salmonids. This Special Issue of Reviews in Fish Biology and Fisheries includes five papers that were submitted to the symposium.

Reviews in Fish Biology and Fisheries↗

Evaluation of genetic population structure of smallmouth bass in the Susquehanna River basin, Pennsylvania

The Smallmouth Bass Micropterus dolomieu was introduced into the Susquehanna River basin, Pennsylvania, nearly 150 years ago. Since introduction, it has become an economically and ecologically important species that supports popular recreational fisheries. It is also one of the most abundant top predators in the system. Currently, there is no information on the level of genetic diversity or genetic structuring that may have occurred since introduction. An understanding of genetic diversity is important for the delineation of management units and investigation of gene flow at various management scales. The goals of this research were to investigate population genetic structure of Smallmouth Bass at sites within the Susquehanna River basin and to assess genetic differentiation relative to Smallmouth Bass at an out-of-basin site (Allegheny River, Pennsylvania) located within the species’ native range. During spring 2015, fin clips ( n = 1,034) were collected from adults at 11 river sites and 13 tributary sites in the Susquehanna River basin and at one site on the Allegheny River. Fin clips were genotyped at 12 polymorphic microsatellite loci. Based on our results, adults sampled throughout the Susquehanna River basin did not represent separate genetic populations. There were only subtle differences in genetic diversity among sites (mean pairwise genetic differentiation index F ST = 0.012), and there was an overall lack of population differentiation ( K = 3 admixed populations). The greatest genetic differentiation was observed between fish collected from the out-of-basin site and those from the Susquehanna River basin sites. Knowledge that separate genetic populations of Smallmouth Bass do not exist in the Susquehanna River basin is valuable information for fisheries management in addition to providing baseline genetic data on an introduced sport fish population.

Pennsylvania↗

Predation of Lost River and Shortnose suckers by piscivorous colonial waterbirds in the Upper Klamath Basin: An analysis of predation effects during 2021–2023

Previously published research indicated that predation by piscivorous colonial waterbirds in the Upper Klamath Basin was a source of mortality for Lost River suckers ( Deltistes luxatus ) and Shortnose suckers ( Chasmistes brevirostris ), including mortality of Sucker Assisted Rearing Program (SARP) fish. Avian predation on recently released Chinook Salmon ( Oncorhynchus tshawytscha ) in the Upper Klamath Basin has not been studied. To provide fisheries managers with the most up-to-date information, we estimated predation rates on passive integrated transponder tagged (PIT) suckers and Chinook Salmon by breeding colonies of American White Pelicans ( Pelecanus erythrorhynchos ), Double-crested Cormorants ( Nannopterum auritum ), Caspian Terns ( Hydroprogne caspia ), California Gulls ( Larus californicus ), Ring-billed Gulls ( L. delawarensis ), Great Blue Herons ( Ardea herodias ), and Great Egrets ( A. alba ) during 2021–2023. Predation rate estimates were variable depending on the fish species, age-class (juvenile, adult), waterbody (Upper Klamath Lake, Clear Lake Reservoir, Sheepy Lake), and year. Results indicated that avian predation rates were highest on juvenile suckers and on suckers in Clear Lake Reservoir. SARP fish released in the spring/summer were more likely to be consumed by breeding birds than those released in the fall/winter, although the number of fish released in the fall/winter that survived to the spring/summer was unknown and could bias predation estimates low. Predation rate estimates on Chinook Salmon varied by year, release timing (spring/summer, fall/winter), release location (Upper Klamath Lake, Klamath River), and age-class (subyearling, yearling). The location and size of colonies were also highly variable depending on the bird species, colony location, and year. Future research could focus on identifying biotic and abiotic factors associated with sucker susceptibility to piscivorous colonial waterbirds and determining to what degree avian predation limits the recovery of suckers in the Upper Klamath Basin.

California, Oregon↗

Second fin ray shows promise for estimating ages of juvenile but not adult Lake Sturgeon

The first marginal pectoral fin ray (fin spine) is the most common structure used for estimating the age of sturgeons, including Lake Sturgeon Acipenser fulvescens . However, conflicting results from studies on the effects of fin spine removal have made some managers hesitant about the practice. We investigated whether the second pectoral fin ray, which can be removed in a less invasive procedure, could be used for estimating ages of Lake Sturgeon. Ages estimated from fin spine and second fin ray samples were compared for 53 wild (470 to 1,981 mm TL) and 16 stocked, known-age (ages 8–18) Lake Sturgeon. Mean coefficient of variation for all samples was 12.4% for the fin spine and 17.5% for the second fin ray. In known-age fish, 17% of estimated ages for the fin spine and the second fin ray matched true age. For the remaining estimates, the difference between the second fin ray and true age was greater than the difference between the fin spine and true age ( P < 0.05, Wilcoxon’s signed rank test). In juvenile fish ( n = 24), 75% of ages estimated from fin spines and second fin rays were within ±4 annuli, which was similar to differences in reader agreement for the same fin spine. Age estimates for adult Lake Sturgeon ( n = 45) were less when using the second fin ray relative to the fin spine (up to –34 years). Additionally, poor annulus clarity was observed in >70% of the second fin rays sampled from adult fish. Our results suggest that the second fin ray does not provide reliable age estimates for adult Lake Sturgeon but may have some utility for estimating age of juvenile Lake Sturgeon. Additional research with a larger sample size would be required to provide more conclusive results.

Vermont, Wisconsin↗

Nine microsatellite loci developed from the octocoral, Paragorgia arborea

Paragorgia arborea, or bubblegum coral, occurs in continental slope habitats worldwide, which are increasingly threatened by human activities such as energy development and fisheries practices. From 101 putative loci screened, nine microsatellite markers were developed from samples taken from Baltimore canyon in the western North Atlantic Ocean. The number of alleles ranged from two to thirteen per locus and each displayed equilibrium. These nuclear resources will help further research on population connectivity in threatened coral species where mitochondrial markers are known to lack fine-scale genetic diversity.

Baltimore Canyon↗

Does incorporating gear selectivity during macroscale investigations of fish growth reduce size-selective sampling bias in parameter estimates?

Understanding of fish growth, the spatial variability in individual growth, and the potential drivers of such variability is a fundamental component of many ecological investigations. However, sampling gears are always size-selective, and this selectivity can result in biased parameter estimates that can lead to, for example, biased stock assessments that use growth estimates. Using seven flathead catfish ( Pylodictis olivaris ) populations from across the USA as an example, we investigated to what degree the incorporation of gear selectivity in growth models reduces size-selective bias in the estimation of growth parameters during macroscale investigations of fish growth. We developed a series of simulation scenarios by combining different sampling methods to obtain fish samples and different gear selectivity assumptions to estimate parameters. Results showed that the efficacy of incorporating gear selectivity in growth models to reduce size-selective sampling bias during macroscale investigations depends on multiple factors, including ( i ) the size distribution of small and large fish in the sample (which is a function of sampling methods), and ( ii ) consistency of sampling methods across populations. Incorporation of gear selectivity may reduce bias when data are lacking for large fish and when sampling methods are inconsistent across populations. Demographics of the sampled populations and the growth parameter of interest can also affect the utility of directly incorporating gear selectivity into growth models. Because multiple factors can influence the efficacy of incorporating gear selectivity into growth models, the decision to do so likely needs to be made on a case-by-case basis. This study extends the existing gear selectivity research by focusing on macroscale fish growth investigations across multiple populations and provides guidance on how to handle gear selectivity assumptions during such investigations.

Canadian Journal Fisheries and Aquatic Sciences↗

Spatial and temporal patterns of nonindigenous fish introductions in the United States

In 1978 biologists in Gainesville, Florida, began compiling records on the distribution and status of nonindigenous fishes known in U.S. inland waters. The database, now in electronic format, currently contains approximately 17,000 records representing more than 500 nonindigenous fish taxa (i.e., species, hybrids, and unidentified forms). Of these taxa, 317 (61%) are native to the United States but have been introduced by humans into U.S. drainages outside their natural geographic ranges; 185 (35%) are fishes introduced from foreign countries; and 22 (4%) are hybrids. Of the introduced foreign fish taxa, 71 (38%) are species that have established (i.e., reproducing) or possibly established populations in open U.S. waters. The database is a useful tool for natural resource managers and other decision makers. Although we periodically revise records and constantly enter new ones, our database is fairly updated; thus, we are able to more thoroughly analyze patterns of introduction and the spread of nonindigenous fishes within the United States. Moreover, information gaps exposed by the data set should serve to stimulate and guide future research on nonindigenous fishes. This paper introduces our database and provides an overview of temporal and spatial patterns of nonindigenous fish distributions in U.S. inland waters.

Fisheries↗

Federal-state aquaculture drug registration partnership: A success story in the making

During the past 20 years, aquaculture has grown both as a vital tool for fisheries management and as a viable industry. But now a crisis has arisen from the Food and Drug Administration's (FDA) increased regulation of drug use in aquaculture in response to public concerns about human food safety, human health, and environmental effects. Lack of approved drugs and chemicals has dramatically reduced the effectiveness and increased the cost of fish production for natural resource management agencies. To make badly needed therapeutants available, the FDA is requiring an array of specialized laboratory research studies and clinical field trials. Pharmaceutical manufacturers are reluctant to undertake any major efforts to gain approval of aquaculture drugs because each (i.e., use on one species for one purpose) is estimated to cost a minimum of $3.5 million. Hence, the expenditure is not warranted by the apparent market potential. Only three therapeutants and one anesthetic are currently approved and available to hatchery managers.

Fisheries↗

Protection of fish spawning habitat for the conservation of warm-temperature reef-fish fisheries of shelf-edge reefs of Florida

We mapped and briefly describe the surficial geology of selected examples of shelf-edge reefs (50–120 m deep) of the southeastern United States, which are apparently derived from ancient Pleistocene shorelines and are intermittently distributed throughout the region. These reefs are ecologically significant because they support a diverse array of fish and invertebrate species, and they are the only aggregation spawning sites of gag ( Mycteroperca microlepis ), scamp ( M. phenax ), and other economically important reef fish. Our studies on the east Florida shelf in the Experimental Oculina Research Reserve show that extensive damage to the habitat-structuring coral Oculina varicosa has occurred in the past, apparently from trawling and dredging activities of the 1970s and later. On damaged or destroyed Oculina habitat, reef-fish abundance and diversity are low, whereas on intact habitat, reef-fish diversity is relatively high compared to historical diversity on the same site. The abundance and biomass of the economically important reef fish was much higher in the past than it is now, and spawning aggregations of gag and scamp have been lost or greatly reduced in size. On the west Florida shelf, fishers have concentrated on shelf-edge habitats for over 100 yrs, but fishing intensity increased dramatically in the 1980s. Those reefs are characterized by low abundance of economically important species. The degree and extent of habitat damage there is unknown. We recommend marine fishery reserves to protect habitat and for use in experimentally examining the potential production of unfished communities.

Florida↗

Protection of fish spawning habitat for the conservation of warm temperate reef fish fisheries of shelf-edge reefs of Florida

We mapped and briefly describe the surficial geology of selected examples of shelf-edge reefs (50–120 m deep) of the southeastern United States, which are apparently derived from ancient Pleistocene shorelines and are intermittently distributed throughout the region. These reefs are ecologically significant because they support a diverse array of fish and invertebrate species, and they are the only aggregation spawning sites of gag ( Mycteroperca microlepis ), scamp ( M. phenax ), and other economically important reef fish. Our studies on the east Florida shelf in the Experimental Oculina Research Reserve show that extensive damage to the habitat-structuring coral Oculina varicosa has occurred in the past, apparently from trawling and dredging activities of the 1970s and later. On damaged or destroyed Oculina habitat, reef-fish abundance and diversity are low, whereas on intact habitat, reef-fish diversity is relatively high compared to historical diversity on the same site. The abundance and biomass of the economically important reef fish was much higher in the past than it is now, and spawning aggregations of gag and scamp have been lost or greatly reduced in size. On the west Florida shelf, fishers have concentrated on shelf-edge habitats for over 100 yrs, but fishing intensity increased dramatically in the 1980s. Those reefs are characterized by low abundance of economically important species. The degree and extent of habitat damage there is unknown. We recommend marine fishery reserves to protect habitat and for use in experimentally examining the potential production of unfished communities.

Florida↗

Creating figures in R that meet the AFS style guide: Standardization and supporting script

Visual display of information in scientific and non‐scientific literature is the most efficient way to summarize large amounts data, focus the readers’ attention on patterns, and substantiate the message in the narrative. Figures often represent years of data collection and substantial monetary investment, and it is worth repeating the cliché “a [figure] is worth a thousand words.” Well‐designed figures are usually simple, yet their ability to relate complex information through simplicity makes them powerful (Tufte 2001 ). Figures are often the focal point of articles when scientists, science communicators, and policy makers are quickly searching for scientific information. Scientists in academia ranked figures and tables as the most important component of research articles (Hubbard and Dunbar 2017 ); moreover, figures quickly become the focus when discussing articles among colleagues or in a classroom setting. If created effectively in combination with a well‐articulated figure caption, figures convey complex information readily for the reader to make a conclusion about results without reading details presented in the narrative. This is especially important as the quantity of research articles continues to increase exponentially in the 21st century, as does the number of journals with specific figure guidelines (Jinha 2010 ).

Fisheries Magazine↗

How does climate change affect emergent properties of aquatic ecosystems?

Emergent properties of ecosystems are community attributes, such as structure and function, that arise from connections and interactions (e.g., predator–prey, competition) among populations, species, or assemblages that, when viewed together, provide a holistic representation that is more than the sum of its individual parts. Climate change is altering emergent properties of aquatic ecosystems through component responses, a combination of shifts in species range, phenology, distribution, and productivity, which lead to novel ecosystems that have no historical analog. The reshuffling, restructuring, and rewiring of aquatic ecosystems due to climate impacts are of high concern for natural resource management and conservation as these changes can lead to species extinctions and reductions in ecosystem services. Overall, we found that substantial progress has been made to advance our understanding of how climate change is affecting emergent properties of aquatic ecosystems. However, responses are incredibly complex, and high uncertainty remains for how systems will reorganize and function over the coming decades. This cross‐system perspective summarizes the state of knowledge of climate‐driven emergent properties in aquatic habitats with case studies that highlight mechanisms of change, observed or anticipated outcomes, as well as insights into confounding non‐climate effects, research tools, and management approaches to advance the field.

Fisheries↗

Role of physical barriers in the control of sea lamprey ( Petromyzon marinus )

Mechanical and electromechanical barriers played a significant role in the initial attempts to control sea lamprey ( Petromyzon marinus ) populations in the upper Great Lakes. More recently electromechanical weirs have been used to assess the relative abundance of spawning-run sea lampreys in Lake Superior. Development of an integrated control approach to sea lamprey control has stimulated an ongoing research program to define structural and/or velocity criteria that can be used to design barrier dams that block spawning runs of sea lamprey

Canadian Journal of Fisheries and Aquatic Sciences↗

Detection of PIT-tagged subyearling Chinook salmon at a Snake River dam: Implications for summer flow augmentation

Rearing subyearling chinook salmon Oncorhynchus tshawytscha (≥60 mm in fork length) were captured in the Snake River and tagged with passive integrated transponders to provide an index of their survival to Lower Granite Dam, the first of eight dams encountered by seaward migrants. Water was released from reservoirs upstream of Lower Granite Dam to augment summer flows and thereby increase subyearling chinook salmon survival. Mean summer flow and maximum summer water temperature in Lower Granite Reservoir were highly correlated ( N = 4; r = −0.999). Acknowledging this correlation, we conducted two separate least-squares regressions using detection rate as the dependent variable. Detection rate at Lower Granite Dam was positively related to mean summer flow ( N = 4; r 2 = 0.993; P = 0.003) and negatively related to maximum summer water temperature ( N = 4; r 2 = 0.984; P = 0.008). Summer flow augmentation increased flow and decreased water temperature in Lower Granite Reservoir especially in low-flow years. Our results support summer flow augmentation as a beneficial interim recovery measure for enhancing survival of subyearling chinook salmon in the Snake River. Additional research should include replicate within-year releases of PIT-tagged subyearlings as well as studies of fish guidance efficiency.

Washington↗

Nonlinear response of trout abundance to summer stream temperatures across a thermally diverse montane landscape

Stream temperature is a fundamental physical factor that affects the distribution and abundance of salmonids, but empirical inconsistencies exist regarding the nature of this relationship in wild populations. We sampled trout populations composed primarily of cutthroat trout Oncorhynchus clarki but also including brown trout Salmo trutta and brook trout Salvelinus fontinalis at 102 reaches on 24 first- to fourth-order streams across a thermally diverse montane landscape. Curves fit to scatterplots of density and biomass versus mean July-August stream temperatures suggested nonlinear, dome-shaped responses. Peaks occurred near mean stream temperatures of 12??C; x-intercepts were near 3??C and 21??C. We conclude that inconsistencies in previously reported temperature-abundance relationships for wild trout populations may have resulted from sampling only a subset of the thermal environments occupied by a species. Researchers analyzing this relationship should be cognizant of the range of temperatures studied and the expected form of the relationship over that range.

Transactions of the American Fisheries Society↗

The influence of nutrients from carcasses of sea lamprey (Petromyzon marinus) on larval growth and spawner abundance

Migrations of anadromous sea lamprey (Petromyzon marinus) from marine ecosystems serve as vectors of nutrients into freshwater food webs. Larval sea lamprey reside in streams for 6–8 years as deposit feeders before metamorphosing into juveniles and migrating to the ocean. Previous work has shown that carcass nutrients, which result from the death of adult lamprey after spawning, increase stream productivity and are consumed by larvae. This may increase larval growth rates and enhance earlier metamorphosis. We examined the sensitivity of life-history parameters and influence of nutrients from carcasses of sea lamprey on the age and growth of larval conspecifics with a deterministic stock-recruitment model. We hypothesized that variability in growth among larval populations is productivity mediated and we compared modeled populations in which larvae receive added growth benefits from carcass nutrients with populations that do not. The results of our simulation indicate that increases in larval growth and lower age at metamorphosis over time lead to an increase in spawner abundance. Increased growth rates may also improve fitness and bolster subsequent spawning stocks. Our research characterized 2 potential ecosystem states, one in which larval sea lamprey benefit from adult conspecifics and another in which the larvae do not.

Fishery Bulletin↗