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807 records · Page 37Linked to original sources

Examining the compositional selectivity of hydrocarbon oxidation products using liquid–liquid extraction and solid-phase extraction techniques

The effect of extraction methods on detecting hydrocarbon oxidation products (HOPs) in groundwater remains unclear. HOPs are polar, water-soluble byproducts of petroleum biodegradation. Our previous work showed that liquid–liquid extraction (LLE), a method commonly used in regulatory monitoring, has a significantly lower extraction efficiency for HOPs compared to solid-phase extraction (SPE). In this study, we evaluate the analytical limitations and compositional selectivity of LLE and SPE using groundwater samples from the Bemidji, MN, crude oil spill site. Optical properties were characterized using excitation–emission matrix spectroscopy (EEMs), and a three-component PARAFAC model was validated, showing consistent trends across both extracts and whole water samples. Ultrahigh-resolution mass spectrometry (UHR-MS) revealed that LLE selectively recovered aliphatic-like compounds but underrepresented more polar oxygenated HOPs. In contrast, SPE methods were more effective at isolating highly oxidized compound classes. These differences were consistent across a gradient of contamination. Overall, the LLE was less precise and less representative of polar HOPs, introducing bias in the characterization of HOPs. This study is the first to quantitatively demonstrate the compositional selectivity and analytical bias of LLE versus SPE for HOPs using combined EEM-PARAFAC and UHR-MS techniques, with implications for long-term monitoring and site assessment protocols.

Environmental Science and Technology

Using visualization science to inform the design of environmental decision-support tools—A case study of the U.S. Geological Survey Waterwatch

Environmental decision-support tools are increasingly being used to serve both expert and non-expert audiences. Many existing tools are primarily expert-focused, and redesigning them can be challenging because experts and non-experts interact with tools differently, existing users may be resistant to changes, and there is little guidance on how to prioritize redesign efforts and demonstrate their efficacy. In this report, we present a case study of a user-centered redesign of an established environmental decision-support tool—the U.S. Geological Survey WaterWatch. WaterWatch supports flood, drought, and other water resource management decisions through the display of water levels at gages across the United States. Using a participatory process, we identified a functional change (replacing the existing rainbow colormap), created an alternative design, and tested the alternative’s usability through two general public surveys. The results showed that replacing the rainbow colormap with a more intuitive diverging colormap improves usability, regardless of the audience’s subjective preference for the rainbow color scheme. In addition, we demonstrated the importance of using legends to improve the audience’s understanding of the map symbols. This study demonstrates how user-centered design approaches can be used to inform the design of high-profile products and tools.

Scientific Investigations Report

Evaluation of stream capture related to groundwater pumping, middle Humboldt River Basin, Nevada

Historical, future, and potential stream capture from groundwater pumping in the middle Humboldt River Basin (MHRB), Nevada, is estimated using a calibrated numerical groundwater flow model. The model was developed to estimate (1) stream capture, which is the change in flux between the groundwater system and the Humboldt River and tributaries, and (2) change in streamflow, which is the change in streamflow estimated for the Imlay gage on the Humboldt River (U.S. Geological Survey streamgage 10333000). Historical stream capture for water years (WYs) 1961–2015 is estimated using recorded and estimated groundwater pumping during that period. Future (predictive) stream capture was based on historical stresses (WYs 1961–2015) using a scenario that simulated non-mine pumping from WY 2015 at a uniform rate for 100 years into the future. Potential stream capture throughout the middle Humboldt River Basin from groundwater pumping during varying durations of time are presented in a series of capture maps. Maps also are presented that show the potential to capture from groundwater evapotranspiration, as well as the storage changes for pumping duration of 100 years. Estimates of historical stream capture from the mainstem Humboldt River during the early 1960s are less than 400 acre-feet per year (acre-ft/yr) when groundwater withdrawals and pumping rates were relatively small compared to more recent times. In the late 1980s and early 1990s, groundwater withdrawals increased and estimated historical stream capture also increased from about 4,000 acre-ft/yr in the late 1980s and early 1990s to as much as 18,800 acre-feet (acre-ft) in WY 1998. In WY 2015, estimated historical stream capture declined to about 13,000 acre-ft because of decreasing groundwater withdrawals and lower streamflow during the drought of WYs 2012–15, resulting in less stream water available for capture. Stream capture was estimated for 100 years into the future based on WY 2015 non-mine pumping rates and mine-dewatering activity through WY 2015. Stream capture is forecast to increase to about 23,000 acre-ft/yr, and streamflow in the Humboldt River could decrease by as much as 19,000 acre-ft/yr. Pumping for mine-dewatering and the associated discharge of that water affects streamflow in the Humboldt River at Imlay, Nevada (U.S. Geological Survey streamgage 10333000). Historically, from WYs 1991 to 2015, streamflow was greater at Imlay gage during active mine-dewatering from mine-water discharge operations and increased by as much as 105,000 acre-ft in WY 1998. The increase was attributed mostly to the discharge of groundwater from mine-related dewatering operations directly into the mainstem Humboldt River or its tributaries, with some of this increase associated with return flows from discharge to rapid infiltration basins. Results indicate that streamflow at Imlay gage is expected to decrease by as much as 1,600 acre-ft/yr 30 years after mine-related pumping and discharge are discontinued. The streamflow reductions at the Imlay gage are expected to then decrease to around 500 acre-ft/yr, 100 years after mine-related pumping and discharge are discontinued. Potential capture maps were produced for pumping durations of 10, 25, 50, and 100 years. Capture map results indicate that areas of greater potential stream capture occur adjacent to the Humboldt River and for upstream tributaries areas north of the Humboldt River.

Nevada

Ordovician stratigraphy, structure, and karst of the Falling Spring Valley, Alleghany County, Virginia, USA

This one-day trip highlights new findings on a preliminary bedrock geologic map that shows results from ongoing geologic mapping in the Falling Spring Valley of Alleghany County, Virginia, USA, which is the southern end of the larger Warm Springs Valley, an elongated anticlinal valley rimmed by Ordovician and Silurian siliciclastic rocks, and which is famous for its thermal springs. This mapping includes stratigraphic, structural, and karst field and lab research focused on the Ordovician strata exposed in the area, the oldest of which is the dolomitic upper part of the Beekmantown Formation (Lower Ordovician, Darriwilian), and the youngest of which is the Juniata Formation (Upper Ordovician, Katian), a sequence of siliciclastic redbeds. Warm Springs Valley is the location of the only known caves in the eastern United States—three at present—with thermal waters flowing in some of their passages. Stops on the trip will highlight key details from mapping efforts, primarily within the structurally deformed Ordovician carbonate sequence that is exposed in the core and limbs of the anticline, as well as the associated karst features that are developed in those carbonate rocks, including results of recent dye traces and water temperature monitoring that have improved our understanding of the karst hydrogeologic systems developed in these strata.

Virginia

Seabed maps showing topography, ruggedness, backscatter intensity, sediment mobility, and the distribution of geologic substrates in quadrangle 3 of the Stellwagen Bank National Marine Sanctuary region offshore of Boston, Massachusetts

The U.S. Geological Survey, in cooperation with the National Marine Sanctuary Program of the National Oceanic and Atmospheric Administration, has conducted seabed mapping and related research in the Stellwagen Bank National Marine Sanctuary (SBNMS) region since 1993. The area being mapped using geophysical and geological data includes the SBNMS and the surrounding region, which totals approximately 3,700 square kilometers (km 2 ) and is subdivided into 18 quadrangles. The seabed is a glaciated terrain that is topographically and texturally diverse. Quadrangle 3, the subject of this scientific investigations map, has a mapped area of 185 km 2 and has water depths that range from about 30 meters (m) on the Stellwagen Bank crest to about 135 m in a basin east of South Ninety Bank, which lies off the eastern margin of Stellwagen Bank. Seven map types, each at a scale of 1:25,000, depict seabed topography, ruggedness, backscatter intensity, distribution of geologic substrates, sediment mobility, distribution of fine- and coarse-grained sand, and substrate mud content. These maps show the distribution of geologic substrates on the southeastern part of Stellwagen Bank, on adjacent banks and basins in deeper water to the east, in the eastern part of Race Point Channel to the south of the bank, and on the northern slope of Cape Cod. Interpretations of multibeam sonar bathymetric and seabed backscatter imagery, photographs, video imagery, and grain-size analyses were used to create the geology-based maps. Data from 309 stations were analyzed, including 279 sediment samples. The geologic substrate maps of quadrangle 3 show the distribution of 21 geologic substrates that represent a wide range of textures, such as rippled sand, immobile sand, immobile muddy sand, sand that partially veneers gravel, and boulder ridges. Mapped substrates are characterized by sediment grain-size composition, surface morphology, substrate layering, the mobility or immobility of substrate surfaces, and water depth range. This scientific investigations map portrays the major geological elements (substrates, topographic features, and processes) of environments in quadrangle 3. It is intended to provide a foundation for research into present and past sediment transport processes in a complex terrain, provide insights into the ecological requirements of invertebrate and vertebrate species that use the various substrates, and support seabed management in the region.

Quadrangle 3 of the Stellwagen Bank National Marin

Continental-scale prediction of hydrologic signatures and processes

Understanding how dominant hydrologic processes and their drivers vary across diverse continental-scale landscapes is critical for hydrologic modeling and water management applications. Our research addresses this question by synthesizing large-sample watershed datasets, Caravan and GAGES-II, and developing random forest models to identify patterns in hydrologic function. We assessed dominant processes by examining hydrologic signatures – summary indicators of watershed function derived from hydroclimatic time series and random forest models across 14 146 gauged United States watersheds. The results reveal clear continental-scale gradients in hydrologic processes, including baseflow, overland flow, storage, and water balance losses. Our map of dominant processes highlights, for example, the transition from baseflow to fast responses and back to baseflow along the elevation gradient from the Appalachian spine, through the Piedmont, to the Eastern Coastal Plain; a distinct outer ring around the Great Lakes region; and sharp contrasts between coastal and inland processes in the West. Variable importance analysis from random forest models show that processes in the western U.S. are primarily controlled by climate, whereas in the eastern U.S., soil, geology, and topography play larger roles, with distinct human influences apparent in urban areas. Our approach of estimating dominant processes and their drivers facilitates extending process knowledge from research watersheds to the continental scale, assessing current hydrological understanding, and evaluating hydrological model structures.

conterminous United States

Wetlands, groundwater and seasonality influence the spatial distribution of stream chemistry in a low-relief catchment

Evaluating stream water chemistry patterns provides insight into catchment ecosystem and hydrologic processes. Spatially distributed patterns and controls of stream solutes are well-established for high-relief catchments where solute flow paths align with surface topography. However, the controls on solute patterns are poorly constrained for low-relief catchments where hydrogeologic heterogeneities and river corridor features, like wetlands, may influence water and solute transport. Here, we provide a data set of solute patterns from 58 synoptic surveys across 28 sites and over 32 months in a low-relief wetland-rich catchment to determine the major surface and subsurface controls along with wetland influence across the catchment. In this low-relief catchment, the expected wetland storage, processing, and transport of solutes is only apparent in solute patterns of the smallest subcatchments. Meanwhile, downstream seasonal and wetland influence on observed chemistry can be masked by large groundwater contributions to the main stream channel. These findings highlight the importance of incorporating variable groundwater contributions into catchment-scale studies for low-relief catchments, and that understanding the overall influence of wetlands on stream chemistry requires sampling across various spatial and temporal scales. Therefore, in low-relief wetland-rich catchments, given the mosaic of above and below ground controls on stream solutes, modeling efforts may need to include both surface and subsurface hydrological data and processes.

Michigan

Assessment of groundwater quantity and quality contributions to Lake Huron

Lake Huron, one of the five Great Lakes, borders the United States and Canada, with Michigan as the only U.S. State on its shoreline. Like other freshwater lakes, it faces water-quality challenges from nutrients and chemicals applied across its drainage basin. Although past studies focused on surface-water sources, groundwater contributions remain less understood. To address this gap, the U.S. Geological Survey, as part of the Cooperative Science and Monitoring Initiative, classified drainage basins to Lake Huron into eight hydrogeologic zones based on bedrock rock type and glacial sediment transmissivity. Utilizing existing data and empirical field data, we quantified groundwater discharge and identified areas of concern for loading of chloride and nitrate to Lake Huron. Groundwater contributions, including indirect and shoreline discharge, ranged from 5.8 to 11.5 inches annually, totaling 1.9 cubic miles and 0.09 cubic mile, respectively. Hydrogeologic zones with higher glacial sediment transmissivity yielded greater indirect groundwater discharge. Chloride levels above the U.S. Environmental Protection Agency’s 250-mg/L recommendation were mainly in the Saginaw lowlands, whereas nitrate above the 10-mg/L standard was rare—found in only 11 wells. Together, the analysis of where groundwater discharge is occurring in the Lake Huron Basin and the identification of areas with potential groundwater-quality concerns can help prioritize areas that are critical to protecting the long-term health of Lake Huron.

Michigan

Linking permafrost to the abundance, biomass, and energy density of fish in Arctic headwater streams

Permafrost thaw alters groundwater flow, river hydrology, stream-catchment interactions, and the availability of carbon and nutrients in headwater streams. The impact of permafrost on watershed hydrology and biogeochemistry of headwater streams has been demonstrated, but there is little understanding of how permafrost influences fish in these ecosystems. We examined relations among permafrost characteristics, the resulting changes in water temperature, stream hydrology (e.g., discharge flashiness), and macroinvertebrates, with the abundance, biomass, and energy density of juvenile Dolly Varden ( Salvelinus malma ) and Arctic Grayling ( Thymallus arcticus ) across 10 headwater streams in northwestern Alaska. Macroinvertebrate density was driven by concentrations of dissolved carbon and nutrients supporting stream food webs. Dolly Varden abundance was primarily related to water temperature with fewer fish in warmer streams, whereas Dolly Varden energy density decreased with the flashiness of the headwater streams. Dolly Varden biomass was related to both temperature and bottom-up food web effects. The energy density of Arctic Grayling decreased with warmer temperatures and discharge flashiness. These relations demonstrate the importance of terrestrial–aquatic connections in permafrost landscapes and indicate the complexity of landscape effects on fish. Because permafrost thaw is one of the most impactful changes occurring as the Arctic warms, an improved understanding of how stream temperature, hydrology, and bottom-up food web processes influence fish populations can aid forecasting of future conditions across the Arctic.

Alaska

Systematic approach to prioritize wells for effective groundwater monitoring and management in the Arkansas Headwaters Basin, Colorado, USA

Study region The Arkansas Headwaters Basin, an intermountain basin in the Southern Rocky Mountains of North America. Study focus Our specific focus is choosing a set of wells to support a possible future regional groundwater-surface water model that would support water management. We present a three-step process using multiple criteria to score, predict, and choose prioritized wells that capture the full distribution of data including extremes. The three-step process provides accessible visualizations, fiscally efficient well prioritization, and screening useful for subsequent groundwater modeling. The novelty of the proposed methodology is the systematic approach integrating a scoring and a predictive approach to support a selection path. The systematic approach may be broadly adapted for other basins. New hydrological insights for the region Understanding regional hydrology hinges on efficient collection of hydrologic data that captures the relevant dynamics including extremes. The present study, a case study for a particular basin in the Southern Rocky Mountains, is the first use of a scripted (R software) strategy to select an economical and representative set of monitoring wells. Our findings suggest caution when using proximity as a proxy for correlation, because proximal wells in the same geologic formation and similar depths are not always correlated. In the Arkansas Headwaters Basin, subsurface geology may be less influential on groundwater elevations than broader hydrologic influences, such as regional drought.

Colorado

Hydrogeologic framework and extent of saltwater intrusion in Kings, Queens, and Nassau Counties, Long Island, New York

In 2016, the U.S. Geological Survey began a multiyear cooperative study with the New York State Department of Environmental Conservation to evaluate the sustainability of Long Island’s sole-source aquifer system through hydrogeologic mapping, compilation of groundwater chloride concentrations, and groundwater flow modeling. In the initial phase of the islandwide study, the hydrogeologic framework and extent of saltwater intrusion in aquifers in Kings, Queens, and Nassau Counties on western Long Island, N.Y., were investigated. The aquifer system underlying western Long Island has been under stress from pumping of public, irrigation (golf course), and industrial supply wells. Saltwater intrusion has occurred from surrounding embayments (East River, Long Island Sound, Jamaica Bay, and the Atlantic Ocean) due to pumping. Eighteen boreholes were drilled and cores taken during 2019–21 to collect hydrogeologic, geochemical, and geophysical data to delineate the complex subsurface hydrogeology and extent of saltwater intrusion within the study area. Evaluation of the new cores, reexamination of legacy core descriptions, and analysis of borehole geophysical logs was used to refine the previously published hydrogeologic framework of Pleistocene and Cretaceous unconsolidated sediments in the area, including delineation of a previously undefined hydrogeologic unit between the Magothy aquifer and the Raritan confining unit, herein named the “upper Raritan aquifer.” The upper Raritan aquifer was first recognized in southeastern Nassau County from an analysis of about 50 closely spaced boreholes with high-resolution core descriptions and gamma-ray (gamma) logs. Further analysis of borehole logs across the study area indicated that the upper Raritan aquifer was also present in Kings and Queens Counties. Nuclear magnetic resonance (NMR) logging was used for the first time on Long Island to provide estimates of the hydraulic properties of the major aquifer and confining units. Unlike other geophysical logs that record responses to the rock matrix and fluid properties and are strongly dependent on mineralogy, NMR logs record responses to the presence of hydrogen protons in the formation fluid to determine water fraction and pore-size distribution. NMR log analysis provided estimates of the clay-bound, capillary-bound, and mobile water fractions and hydraulic conductivity of aquifers and confining units penetrated by five wells in Nassau County. Pumpage for public-supply and industrial wells on Long Island began in the 1870s with small, localized suppliers of populated areas in Kings and Queens Counties. By 1904–16, pumpage for public water supply in Kings County averaged 21 million gallons per day, and averaged 37 million gallons per day in Queens County, mostly from the upper glacial aquifer. Saltwater intrusion was reported as early as the beginning of the 20th century and included the upper glacial-Jameco-Magothy and Lloyd-North Shore aquifer systems. By 1936, pumping in central Kings County created a major cone of depression in the water table extending to the south shore of much of Kings County and into southwestern Queens County. Saltwater intrusion has caused the shutdown of public-supply wells in Kings, Queens, and Nassau Counties. A large saltwater intrusion wedge in the Lloyd aquifer was indicated in southern Queens County in the early part of the 20th century, and the saltwater interface may have been onshore predevelopment. Most of Kings and Queens Counties are intruded with saltwater in both the upper glacial-Jameco-Magothy and Lloyd-North Shore aquifers systems. Saltwater increased during the 20th century and continues to increase to the present (2023) in the Lloyd-North Shore aquifer system in Great Neck and Manhasset Neck in northern Nassau County. A major wedge of saltwater intrusion in the upper glacial-Jameco-Magothy aquifer in southwestern Nassau County appears to be increasing.

New York

Shallow geologic framework of the Mississippi Sound and the potential for sediment resources

The Mississippi Sound, an estuarine environment located between the mainland and barrier islands bordering the northern Gulf of America (formerly the Gulf of Mexico), serves as a vital ecosystem for the States of Mississippi and Alabama. Spanning approximately 100 kilometers from east to west and covering 1,400 square kilometers, the sound is home to marine industry and ports, and its shallow and brackish waters sustain a diverse array of marine life. Barrier islands along the southern edge of the sound separate the microtidal estuary from the Gulf of America. This protection from gulf wave action mediates current flow within the sound, resulting in predominantly fine-grained sediment deposition along the seafloor. This study, conducted by the U.S. Geological Survey in cooperation with the U.S. Army Corps of Engineers, provides insight on fluvial and tidal processes spanning the past 5,000 years. The report synthesizes existing research to provide a comprehensive overview of the sound geology, from Pleistocene origins to present-day morphology, and utilizes high-resolution single channel seismic profiles and sediment data to identify and map sedimentary deposits and morphologic features at and below the seafloor. Despite its ecological significance, the Mississippi Sound faces environmental challenges, including water-quality issues, habitat degradation, storm-induced erosion, and the ongoing threats of sea-level rise and environmental changes. This study uses the present-day understanding of the sound's geology to inform coastal management decisions, hazard assessment, and potential mineral resources.

Louisiana, Mississippi

Linking stream-reach nitrogen loads and groundwater “reachsheds” to inform wastewater-nitrogen management actions, Cape Cod, Massachusetts

Study Region Cape Cod, Massachusetts, U.S.A. Study Focus Anthropogenic nitrogen (N) is a key factor in degrading groundwater and surface-water quality, particularly in coastal New England where onsite wastewater systems are prevalent. This study evaluated whether direct N-load measurements in streams on Cape Cod, Massachusetts, coupled with flow-path information from groundwater-flow models, can effectively identify potential land areas where nitrogen mitigation could substantially reduce loads to receiving waters. Nitrogen fluxes were measured along 63 stream reaches during winter and summer and paired with simulated groundwater recharge areas to identify and rank potential areas for reduction of nonpoint-source N inputs. New Hydrologic Insights for the Region Reach-scale nitrate-N loads ranged from −39.1–1182 kg-N/yr per 100 m of stream, indicating spatially variable groundwater inputs across seasons. “Reachsheds” — areas contributing groundwater recharge to specific stream reaches — were delineated using a regional groundwater-flow model. Strong correlations were found between observed N loads and land-use characteristics, especially the number of septic systems and total N inputs from the sum of considered sources. Observed N loads were moderately correlated with recharge area size and wastewater flow estimates. Correlating reach-specific groundwater N loads with land use and parcel-scale nitrogen-yield data identified reachsheds with the highest potential for N load reduction. This approach enables targeted implementation of restoration efforts to optimize nutrient management and support regional load reduction.

Massachusetts

A new groundwater energy transport model for the MODFLOW hydrologic simulator

Heat transport in the subsurface is an important aspect of research related to the effects of a warming climate on ecological services (i.e., cold-water refugia); the development of geothermal resources for energy banking schemes (i.e., aquifer thermal energy storage [ATES]); and the effects of temperature on other aspects of groundwater quality, such as nutrient cycling. Historically, simulation of heat transport using the MODFLOW groundwater simulator and related codes was performed by scaling the input parameters of a solute-transport model to emulate heat transport. However, that approach required additional pre- and post-processing of input and output and could not account for the variation in effective thermal storage and transport properties during transient, unsaturated flow, for example. True heat-transport capabilities in the context of MODFLOW were first introduced in a variant called USG-Transport. More recently, a new groundwater energy-transport (GWE) model type has been added to MODFLOW 6, the core version of the MODFLOW hydrologic simulator. GWE supports the simulation of heat transport on structured or unstructured grids as well as within and between features of advanced packages that represent streams, lakes, multi-aquifer wells, and the unsaturated zone. GWE is integrated within MODFLOW 6 and is accessible through the FloPy Python package and the MODFLOW 6 application programming interface (API). An example simulation demonstrates conduction between grid cells through both the water and the solid aquifer material, including thermal bleeding from saturated overburden cells into a groundwater flow field.

Groundwater

Satellite imagery of the north-central Gulf of Mexico, June 1993-May 1997

This CD contains imagery collected by the Advanced Very High Resolution Radiometer (AVHRR) on the NOAA polar-orbiting weather satellites. The AVHRR provides almost daily coverage of a site at a resolution of approximately 1 km. The data types included are sea surface temperature (SST), water reflectance (REF), and a false-color infrared overview (FCI).

Alabama, Florida, Louisiana, Mississippi

Deep groundwater total dissolved solids mapping in the Dakota Group, Williston Basin, USA

Growing concern about the quantity of available freshwater around the world has led to interest in surveying groundwater total dissolved solids (TDS) below water well depths. Deep TDS has not been systematically mapped, and there is much to learn about the distribution and controls on deeper groundwater. In sedimentary basins across the United States, groundwater resources often overlie hydrocarbon resources, providing an opportunity to use borehole geophysical data collected for hydrocarbons to characterize groundwater and pore space resources. This study adapts a recently developed subsurface geostatistical and geophysical modeling approach to continuously map groundwater TDS, porosity, and temperature in the Dakota Group of the Williston Basin—an undercharacterized regional aquifer system overlying deeper hydrocarbon reservoirs. Groundwater TDS in the Dakota Group ranges from approximately 4800 to 26,900 mg/L. TDS patterns are stratified with higher TDS in the lower and upper Dakota Group, and relatively lower TDS in the middle Dakota Group. The lower TDS in the middle zone may represent a preferential regional flow path for lower-TDS meteoric recharge from the west. The alternating pattern of TDS may also be evidence of higher-TDS inflows into the Dakota Group from underlying and potentially from overlying aquifers. Porosity is lower near the center of the Williston Basin and tends to be higher to the east, which may be related to grain size distributions. The new regional TDS and porosity modeling serves as a quantitative reference for water users and provides supporting evidence for hypotheses on Dakota Group recharge.

Montana, North Dakota, South Dakota

Expression of corticoid-regulatory genes in the gills of Atlantic salmon (Salmo salar) parr and smolt and during salinity acclimation

In teleost fishes, cortisol is the major corticoid and has both glucocorticoid and mineralocorticoid actions. However, how fish tissues discriminate between these distinct corticosteroid actions is unclear. In mammals, the major factors responsible for intracellular corticosteroid regulation are glucocorticoid receptors ( gr s) and the mineralocorticoid receptor ( mr ), but their role in osmoregulation of fish is unclear. 11β-hydroxysteroid dehydrogenases ( hsd11b s) control the levels of intracellular corticosteroids by converting from bioactive forms to inert forms. To investigate how Atlantic salmon ( Salmo salar ) respond to cortisol in different physiological or environmental conditions, we performed comparisons of parr and smolt, and osmotic challenge experiments to examine the physiological responses and gill transcript levels of genes underlying cortisol-signalling, including gr1 , gr2 , mr , hsd11b2 and hsd11b3 . Because cortisol may interact with growth hormone and prolactin during salinity changes, transcript levels encoding growth hormone receptors ( ghr1 , ghr2 ) and the prolactin receptor ( prlr ) were also examined. Hsd11b2 transcript levels in seawater-acclimated fish were consistently lower compared to fish acclimated to fresh water. After transfer to seawater, prlr transcript levels in fish significantly decreased and transcript levels of ghr1 , ghr2 and hsd11b3 showed no change or were slightly higher than those of freshwater control groups. Gr1 , gr2 and mr transcript levels were slightly but consistently higher in fish acclimated to fresh water relative to seawater. Our results indicate that changes in corticosteroid receptor and hsd11b2 transcript levels in the gills may be important mechanisms that regulate corticoid signals to achieve ion homeostasis in Atlantic salmon.

Journal of Fish Biology

High resolution identification and quantification of diffuse deep groundwater discharge in mountain rivers using continuous boat-mounted helium measurements

Discharge of deeply sourced groundwater to streams is difficult to locate and quantify, particularly where both discrete and diffuse discharge points exist, but diffuse discharge is one of the primary controls on solute budgets in mountainous watersheds. The noble gas helium is a unique identifier of deep groundwater discharge because groundwater with long residence times is commonly enriched in helium. In this study, a portable mass spectrometer was used to measure longitudinal variation in dissolved helium concentrations in two mountainous rivers at high spatial resolution not feasible with traditional sampling techniques. Helium profiles were then simulated using a mass-balance model to quantify longitudinal variation in groundwater discharge to the receiving rivers. Results indicate helium concentrations were enriched by multiple orders of magnitude above atmospheric equilibrium in both rivers and that this persisted for up to 18 km below observed pulse inputs in the Colorado River. Helium mass-balance models match observed longitudinal patterns with the exception of sharp initial increases in helium observed in the rivers. Increased longitudinal groundwater discharge rates correspond to mapped geologic structures in both watersheds that likely transport deep geothermal water. Models show variable sensitivity to spatial assignment of input variables representing the groundwater source, illustrating the importance of collecting data from discrete groundwater discharges where possible. The methodology shows promise for field experiments designed to assess air–water exchange rates and to quantify total groundwater discharge from a combination of discrete and diffuse sources.

Colorado, Utah