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Temperature

Stream temperature has direct and indirect effects on stream ecology and is critical in determining both abiotic and biotic system responses across a hierarchy of spatial and temporal scales. Temperature variation is primarily driven by solar radiation, while landscape topography, geology, and stream reach scale ecosystem processes contribute to local variability. Spatiotemporal heterogeneity in freshwater ecosystems influences habitat distributions, physiological functions, and phenology of all aquatic organisms. In this chapter we provide an overview of methods for monitoring stream temperature, characterization of thermal profiles, and modeling approaches to stream temperature prediction. Recent advances in temperature monitoring allow for more comprehensive studies of the underlying processes influencing annual variation of temperatures and how thermal variability may impact aquatic organisms at individual, population, and community based scales. Likewise, the development of spatially explicit predictive models provide a framework for simulating natural and anthropogenic effects on thermal regimes which is integral for sustainable management of freshwater systems.

Book chapter↗

Differential invasion success of salmonids in southern Chile: patterns and hypotheses

Biological invasions create complex ecological and societal issues worldwide. Most of the knowledge about invasions comes only from successful invaders, but less is known about which processes determine the differential success of invasions. In this review, we develop a framework to identify the main dimensions driving the success and failure of invaders, including human influences, characteristics of the invader, and biotic interactions. We apply this framework by contrasting hypotheses and available evidence to explain variability in invasion success for 12 salmonids introduced to Chile. The success of Oncorhynchus mykiss and Salmo trutta seems to be influenced by a context-specific combination of their phenotypic plasticity, low ecosystem resistance, and propagule pressure. These well-established invaders may limit the success of subsequently introduced salmonids, with the possible exception of O. tshawytscha, which has a short freshwater residency and limited spatial overlap with trout. Although propagule pressure is high for O. kisutch and S. salar due to their intensive use in aquaculture, their lack of success in Chile may be explained by environmental resistance, including earlier spawning times than in their native ranges, and interactions with previously established and resident Rainbow Trout. Other salmonids have also failed to establish, and they exhibit a suite of ecological traits, environmental resistance, and limited propagule pressure that are variably associated with their lack of success. Collectively, understanding how the various drivers of invasion success interact may explain the differential success of invaders and provide key guidance for managing both positive and negative outcomes associated with their presence.

Reviews in Fish Biology and Fisheries↗

NASA's surface biology and geology designated observable: A perspective on surface imaging algorithms

The 2017–2027 National Academies' Decadal Survey, Thriving on Our Changing Planet , recommended Surface Biology and Geology (SBG) as a “Designated Targeted Observable” (DO). The SBG DO is based on the need for capabilities to acquire global, high spatial resolution, visible to shortwave infrared (VSWIR; 380–2500 nm; ~30 m pixel resolution) hyperspectral (imaging spectroscopy) and multispectral midwave and thermal infrared (MWIR: 3–5 μm; TIR: 8–12 μm; ~60 m pixel resolution) measurements with sub-monthly temporal revisits over terrestrial, freshwater, and coastal marine habitats. To address the various mission design needs, an SBG Algorithms Working Group of multidisciplinary researchers has been formed to review and evaluate the algorithms applicable to the SBG DO across a wide range of Earth science disciplines, including terrestrial and aquatic ecology, atmospheric science, geology, and hydrology. Here, we summarize current state-of-the-practice VSWIR and TIR algorithms that use airborne or orbital spectral imaging observations to address the SBG DO priorities identified by the Decadal Survey: (i) terrestrial vegetation physiology, functional traits, and health; (ii) inland and coastal aquatic ecosystems physiology, functional traits, and health; (iii) snow and ice accumulation, melting, and albedo; (iv) active surface composition (eruptions, landslides, evolving landscapes, hazard risks); (v) effects of changing land use on surface energy, water, momentum, and carbon fluxes; and (vi) managing agriculture, natural habitats, water use/quality, and urban development. We review existing algorithms in the following categories: snow/ice, aquatic environments, geology, and terrestrial vegetation, and summarize the community-state-of-practice in each category. This effort synthesizes the findings of more than 130 scientists.

Remote Sensing of Environment↗

Aquatic ecology of the Elwha River estuary prior to dam removal: Chapter 7 in Coastal habitats of the Elwha River, Washington--biological and physical patterns and processes prior to dam removal

The removal of two long-standing dams on the Elwha River in Washington State will initiate a suite of biological and physical changes to the estuary at the river mouth. Estuaries represent a transition between freshwater and saltwater, have unique assemblages of plants and animals, and are a critical habitat for some salmon species as they migrate to the ocean. This chapter summarizes a number of studies in the Elwha River estuary, and focuses on physical and biological aspects of the ecosystem that are expected to change following dam removal. Included are data sets that summarize (1) water chemistry samples collected over a 16 month period; (2) beach seining activities targeted toward describing the fish assemblage of the estuary and migratory patterns of juvenile salmon; (3) descriptions of the aquatic and terrestrial invertebrate communities in the estuary, which represent an important food source for juvenile fish and are important water quality indicators; and (4) the diet and growth patterns of juvenile Chinook salmon in the lower Elwha River and estuary. These data represent baseline conditions of the ecosystem after nearly a century of changes due to the dams and will be useful in monitoring the changes to the river and estuary following dam removal.

Washington↗

Vegetation and hydrology of land-margin ecosystems: the mangroves of South Florida in relation to disturbance, global change and response to restoration

The USGS Florida Caribbean Science Center's Restoration Ecology Branch and Florida International University is conducting research on disturbance, global change and restoration of land margin ecosystems of South Florida. Criticial research for the restoration of these systems involves understanding the responses of mangrove forests to changes in the quality, quantity, timing and distribution of freshwater inflows, response to global change (e.g. sea level rise) and catastrophic disturbances such as hurricanes.

Florida↗

Summary of findings about circulation and the estuarine turbidity maximum in Suisun Bay, California

Suisun Bay, California, is the most landward subembayment of San Francisco Bay (fig. 1) and is an important ecological habitat (Cloern and others, 1983; Jassby and others, 1995). During the 1960s and 1970s, data collected in Suisun Bay were analyzed to develop a conceptual model of how water, salt, and sediment move within and through the Bay. This conceptual model has been used to manage freshwater flows from the Sacramento-San Joaquin Delta to Suisun Bay to improve habitat for several threatened and endangered fish species. Instrumentation used to measure water velocity, salinity, and suspended-solids concentration (SSC) greatly improved during the 1980s and 1990s. The U.S. Geological Survey (USGS) has utilized these new instruments to collect one of the largest, high-quality hydrodynamic and sediment data sets available for any estuary. Analysis of these new data has led to the revision of the conceptual model of circulation and sediment transport in Suisun Bay.

Fact Sheet↗

Summary of oceanographic and water–quality measurements in West Falmouth Harbor and Buzzards Bay, Massachusetts, 2009–2010

This data report presents oceanographic and water-quality observations made at six locations in West Falmouth Harbor and Buzzards Bay, Massachusetts, from August 2009 to September 2010. Both Buzzards Bay and West Falmouth Harbor are estuarine embayments; the input of freshwater on the eastern margin of Buzzards Bay adjacent to Cape Cod and West Falmouth Harbor is largely due to groundwater. In West Falmouth Harbor, the groundwater that seeps into the harbor is characterized by relatively high levels of nitrate. This high nitrate load has modified the ecology of the harbor (Howes and others, 2006) and may be a significant source of nitrate to Buzzards Bay during seasons with low biological nitrate uptake. The U.S. Geological Survey undertook these measurements to improve understanding of circulation, residence time, and water quality in the harbor and bay. We set up and monitored multiple sites in both Buzzards Bay and West Falmouth Harbor, measuring depth, water velocity,salinity, pH, dissolved oxygen, chlorophyll-a, and nitrate concentration. In this report we present the processed time-series data at these locations and provide access to the data and metadata. The results will be used to understand circulation mechanisms and verify numerical models of hydrodynamics and biogeochemistry.

Massachusetts↗

Effect of analytical conditions in wavelength dispersive electron microprobe analysis on the measurement of strontium-to-calcium (Sr/Ca) ratios in otoliths of anadromous salmonids

The use of strontium-to-calcium (Sr/Ca) ratios in otoliths is becoming a standard method to describe life history type and the chronology of migrations between freshwater and seawater habitats in teleosts (e.g. Kalish, 1990; Radtke et al., 1990; Secor, 1992; Rieman et al., 1994; Radtke, 1995; Limburg, 1995; Tzeng et al. 1997; Volk et al., 2000; Zimmerman, 2000; Zimmerman and Reeves, 2000, 2002). This method provides critical information concerning the relationship and ecology of species exhibiting phenotypic variation in migratory behavior (Kalish, 1990; Secor, 1999). Methods and procedures, however, vary among laboratories because a standard method or protocol for measurement of Sr in otoliths does not exist. In this note, we examine the variations in analytical conditions in an effort to increase precision of Sr/Ca measurements. From these findings we argue that precision can be maximized with higher beam current (although there is specimen damage) than previously recommended by Gunn et al. (1992).

Fishery Bulletin↗

Restoring the Florida Everglades: Insights on integrating sea level rise into decision-support tools

Although coastal ecosystems are impacted by climate change and sea-level rise, many ecological and hydrological models do not yet incorporate sea-level rise projections in their modeling outputs. Therefore, this research examined the various challenges that may prevent sea-level rise from being effectively incorporated in modeling and decision-support tools. We conducted semi-structured interviews with twenty-six professionals involved in Florida’s Everglades restoration. We applied the Diffusions of Innovations Theory to better understand factors that can impact practitioners’ adoption of newly designed decision-support tools that examine sea-level rise in the freshwater Everglades. The Diffusions of Innovations Theory provided insights into practitioners’ perceptions of these tools. We found that these practitioners have a strong interest in using dynamic decision-support tools to plan for sea-level rise impacts on Everglades restoration, particularly when they receive information at appropriate geographic and temporal scales and are given hands-on tools and training. However, challenges that prevent developing these tools include outdated data, limited organizational capacity and funding, limited use of long-term indicators, uncertainty about climate change impacts on local ecosystems, and lack of integration between hydrological and ecological models. Our research also highlights that greater availability of different types of tools can help to meet the needs of the scientific and non-scientific audiences involved in Everglades restoration.

Florida↗

Evaluating the effect of salinity on a simulated American Crocodile ( Crocodylus acutus ) population with applications to conservation and Everglades restoration

Everglades restoration will alter the hydrology of South Florida, affecting both water depth and salinity levels in the southern fringes of the Everglades, the habitat of the endangered American crocodile ( Crocodylus acutus ). A key question is what the effects of these hydrologic changes will be on the crocodile population. Reliable predictions of the viability of endangered species under a variety of management scenarios are of vital importance in conservation ecology. Juvenile American crocodiles are thought to be sensitive to high salinity levels, suffering reduced mass, and potentially reduced survivorship and recruitment. This could negatively impact the population recovery. We addressed the management issue of how the crocodile population will respond to alterations in hydrology with a spatially explicit individual-based model. The model is designed to relate water levels, salinities, and dominant vegetation to crocodile distribution, abundance, population growth, individual growth, survival, nesting effort, and nesting success. Our analysis shows that Everglades restoration, through its effects on water flow to estuaries, may benefit crocodile populations if increased freshwater flow reduces the chance that regional salinity levels exceed levels where small individuals lose mass. In addition, we conclude that conservation priority should be placed on reducing anthropogenic sources of mortality on large individuals, such as road mortality. Finally, research should focus on estimates of annual survivorship for large individuals.

Florida↗

Groundwater hydrology and chemistry of Jamestown Island, Virginia—Potential effects of tides, storm surges, and sea-level rise on archaeological, cultural, and ecological resources

As the site of the first permanent English settlement in North America in 1607, Jamestown Island, Colonial National Historical Park (COLO), Virginia, contains a rich archaeological record that extends from the Paleoindian period (15,000 to 8,000 years ago) through the 20th century. The island is located on the lower James River near the mouth of Chesapeake Bay. Jamestown Island vegetation is dominated by upland forests surrounded by tidal, freshwater-to-oligohaline marshes. Along the Virginia coast, relative sea-level rise was more than 2.5 times the global average during the 20th century. Consequently, the National Park Service (NPS) has identified COLO as one of the 25 national parks most threatened by climate change. Surface waters across the island are hydraulically connected to the laterally continuous Surficial aquifer. The land-surface altitude of the island is low, with two-thirds of the island less than 5 feet (ft) above the North American Vertical Datum of 1988 (NAVD 88). Consequently, sea-level rise, combined with tides and storm surges, threatens the island and its resources as surface-water and groundwater levels rise, saltwater enters the Surficial aquifer, and groundwater chemistry changes. The impact of sea-level rise on the island’s surface-water resources has been well studied, but groundwater effects have been largely ignored. Quantifying the effects of tides, storm surges, and sea-level rise on groundwater levels and chemistry is essential to developing an effective strategy for managing climate-induced changes. The first step in developing a response strategy includes a parkwide general risk assessment for archaeological sites on the island, so that sites can be prioritized for management actions. The U.S. Geological Survey and the NPS began a study in 2015 to develop a long-term groundwater-monitoring program to evaluate this risk and to develop an updated management strategy. The groundwater-monitoring program consists of 45 wells and piezometers in two individual clusters and three transects across the island in different hydrologic and chemical settings. Samples for water quality were collected from the wells and piezometers from October 2015 through September 2018 at variable time intervals. Results of the monitoring identified disparate hydrologic and chemical responses to saltwater intrusion across the island. Specific conductance (an indicator of salinity) of groundwater beneath several marshes responded differently to changes in James River salinity. Groundwater response to changes in James River specific conductance appeared to be controlled by land-surface altitude and slope, differences in lateral and vertical sediment characteristics, distance from surface waters, and the degree of surface water/groundwater connectivity between channels and the aquifer. Groundwater chemistry data from monitoring wells at Black Point, a low-altitude, upland setting, are in contrast with conditions observed in Island House observation wells, a high-altitude, upland setting. Specific conductance (less than 200 microsiemens per centimeter [μS/cm]) and pH (greater than 5.0) of groundwater beneath much of the uplands that characterize the Island House observation wells are typical of groundwater in noncarbonate sedimentary aquifers recharged by precipitation. At Black Point, specific conductance ranged from 2,490 to 15,200 μS/cm, and pH ranged from 3.1 to 6.6 standard units. At the Black Point observation wells, the most saline and dense water was at the water table rather than deeper in the aquifer, causing a density inversion that persisted throughout the study. The density inversion likely resulted from differences in permeability between the shallow clay and fine-grained sands and the deeper coarse-grained sand and gravel. Groundwater with the lowest pH was at the water table. As saline groundwater flows through organic sediment beneath the marshes, bacterial biodegradation of organic matter creates anoxic conditions. Continued biodegradation concomitantly reduces iron-oxide minerals in the sediment and sulfate in saline water. When oxygen is reintroduced into groundwater, iron and sulfur can reoxidize to form sulfuric acid, locally lowering the pH of the water. This report describes the groundwater monitoring network design, rationale for site selection, monitoring approach, and results of monitoring from October 2015 through September 2018. Maps of inundation at selected water-level altitudes are included to identify the risk to archaeological, cultural, and ecological resources. The monitoring results of the hydrology and chemistry data are interpreted, and the different hydrologic and chemical settings are described. The implications of the study results for management decisions are presented, and suggestions for improving the monitoring network are included.

Virginia↗

Genetic diversity, population structure, and historical demography of a highly vagile and human‐impacted seabird in the Pacific Ocean: The red‐tailed tropicbird, Phaethon rubricauda

Many seabird breeding colonies have recovered from heavy anthropogenic disturbance after conservation actions. The widely distributed red‐tailed tropicbird, Phaethon rubricauda , was used as a model species to assess potential anthropogenic impacts on the genetic diversity of breeding colonies in the Pacific Ocean. Cytochrome c oxidase subunit I and control region sequences analyses were conducted across the range of the species in the Pacific Ocean. The study sites were at islands without human‐related disturbance (non‐impacted islands) and with human‐related disturbance (impacted islands). We hypothesized that (i) breeding colonies of the red‐tailed tropicbird on impacted islands have lower genetic diversity compared with colonies on non‐impacted islands, and (ii) breeding colonies of the red‐tailed tropicbird show significant fine and broad‐scale genetic structure across the Pacific Ocean. Bayesian skyline analyses were conducted to infer past changes in population sizes. Genetic diversity was similar between impacted and non‐impacted islands. There was significant broad‐scale genetic structure among colonies separated by over 6,000 km, but a lack of significant fine‐scale genetic structure within Australasia and Hawai'i, although a significant level of differentiation was found within Chile with Φ ST analyses. Skyline analyses showed that effective population sizes remained relatively constant through time, but experienced either a slight decrease or the end of an expansion event through the last 1,000 years. These changes may be related to the arrival of humans on Pacific islands. Impacted islands may have received immigrants from other relatively close islands, buffering the loss of genetic diversity. However, it is also possible that colonies have retained ancestral variation or that a large effective population size coupled with a long generation time (13 years) has prevented the loss of genetic diversity in human‐impacted islands. Future research using higher‐resolution markers is needed to resolve the population genetic structure of the red‐tailed tropicbird in an ecological time‐scale.

Hawaii↗

Oases of the future? Evaluating springs as potential hydrologic refugia in drying climates

Springs in water-limited landscapes are biodiversity hotspots and keystone ecosystems, disproportionately influencing surrounding landscapes despite their often small areas. Some springs served as evolutionary refugia during previous climate drying, supporting relict species in isolated habitats. Understanding whether springs will provide hydrologic refugia from future climate change is important to biodiversity conservation but complicated by hydrologic variability among springs, data limitations, and multiple non-climate threats to groundwater-dependent ecosystems. Here, we present a conceptual framework for categorizing springs as potentially stable, relative, or transient hydrologic refugia in a drying climate. Clues about refugial capacity of springs can be assembled from diverse approaches, including citizen-science-powered ecohydrologic monitoring, remote sensing, landowner interviews, and environmental tracer analysis. Managers can integrate multiple lines of evidence to predict which springs may become future refugia for species of concern, strengthening the long-term effectiveness of springs conservation and restoration and informing climate adaptation for terrestrial and freshwater species.

Frontiers in Ecology and the Environment↗

Effects of latitude, season, and temperature on Lake Sturgeon movement

Ecologists have a limited understanding of the rangewide variation in movement behavior in freshwater fishes, but recent expansion of biotelemetry allows biologists to investigate how fish movement can help to predict behavioral shifts in response to changing environments. The Lake Sturgeon Acipenser fulvescens is a wide-ranging, migratory, coolwater species, making it a candidate species for studying patterns in movement ecology. We conducted a literature review and meta-analysis of seasonal movement data compiled from 38 Lake Sturgeon telemetry studies in North America to build an understanding of spatiotemporal variation in movement behavior. Lake Sturgeon movement studies were underrepresented for low-latitude populations in the Mississippi, Missouri, and Ohio/Tennessee River basins. Highest movement usually occurred during spring spawning migrations, whereas other populations exhibited their highest movement in the fall, potentially in order to overwinter near spawning grounds. Our meta-regression analysis indicated that latitude or summer maximum temperature best predicted standardized mean difference between spring and summer movement in Lake Sturgeon populations across the species’ range. Our results suggest that Lake Sturgeon populations at lower latitudes and those that experience warmer summer temperatures have lower summer movement relative to spring movement. Managers may use this information to prioritize actions promoting the conservation of seasonally important habitats for the continued recovery of migratory coolwater fish (e.g., Lake Sturgeon) that potentially experience stressful thermal conditions.

North American Journal of Fisheries Management↗

Food web perspectives and methods for riverine fish conservation

Food web analyses offer useful insights into understanding how species interactions, trophic relationships, and energy flow underpin important demographic parameters of fish populations such as survival, growth, and reproduction. However, the vast amount of food web literature and the diversity of approaches can be a deterrent to fisheries practitioners engaged in on-the-ground research, monitoring, or restoration. Incorporation of food web perspectives into contemporary fisheries management and conservation is especially rare in riverine systems, where approaches often focus more on the influence of physical habitat and water temperature on fish populations. In this review, we first discuss the importance of food webs in the context of several common fisheries management issues, including assessing carrying capacity, evaluating the effects of habitat change, examining species introductions or extinctions, considering bioaccumulation of toxins, and predicting the effects of climate change and other anthropogenic stressors on riverine fishes. We then examine several relevant perspectives: basic food web description, metabolic models, trophic basis of production, mass-abundance network approaches, ecological stoichiometry, and mathematical modeling. Finally, we highlight several existing and emerging methodologies including diet and prey surveys, eDNA, stable isotopes, fatty acids, and community and network analysis. Although our emphasis and most examples are focused on salmonids in riverine environments, the concepts are easily generalizable to other freshwater fish taxa and ecosystems.

Wiley Interdisciplinary Reviews (WIREs): Water↗

Long-term streamflow trends in Hawai‘i and implications for native stream fauna

Climate change has fundamentally altered the water cycle in tropical islands, which is a critical driver of freshwater ecosystems. To examine how changes in streamflow regime have impacted habitat quality for native migratory aquatic species, we present a 50‐year (1967–2016) analysis of hydrologic records in 23 unregulated streams across the five largest Hawaiian Islands. For each stream, flow was separated into direct run‐off and baseflow and high‐ and low‐flow statistics (i.e., Q10 and Q90) with ecologically important hydrologic indices (e.g., frequency of flooding and low flow duration) derived. Using Mann–Kendall tests with a running trend analysis, we determined the persistence of streamflow trends through time. We analysed native stream fauna from ~400 sites, sampled from 1992 to 2007, to assess species richness among islands and streams. Declines in streamflow metrics indicated a general drying across the islands. In particular, significant declines in low flow conditions (baseflows), were experienced in 57% of streams, compared with a significant decline in storm flow conditions for 22% of streams. The running trend analysis indicated that many of the significant downward trends were not persistent through time but were only significant if recent decades (1987–2016) were included, with an average decline in baseflow and run‐off of 10.90% and 8.28% per decade, respectively. Streams that supported higher native species diversity were associated with moderate discharge and baseflow index, short duration of low flows, and negligible downward trends in flow. A significant decline in dry season flows (May–October) has led to an increase in the number of no‐flow days in drier areas, indicating that more streams may become intermittent, which has important implications for mauka to makai (mountain to ocean) hydrological connectivity and management of Hawai'i's native migratory freshwater fauna.

Hawaii↗

Environmental setting, water quality, and ecological indicators of surface-water quality in the Mermentau River Basin, southwestern Louisiana, 1998-2001

The U.S. Geological Survey collected data from 29 wells and 24 surface-water sites in the Mermentau River Basin, 1998-2001, to better understand ground-water and surface-water quality; aquatic invertebrate communities; and habitat conditions, in relation to land use. This study was apart of the National Water-Quality Assessment Program, which was designed to assess water quality as it relates to various land uses. Water-quality data were evaluated with criteria established for the protection of drinking water and aquatic life, and bed-sediment data were compared to aquatic life criteria. Water-quality and ecological data were analyzed statistically in relation to drainage area and agricultural land-use integrity. Concentrations of nutrients and major inorganic ions in ground water and surface water generally were highest in the southeastern part of the study area where soils contain thick loess deposits. Peak concentrations of nutrients in surface water occurred March-may at two sites with high agricultural intensity; the lowest concentrations occurred August-January. The greatest potential for eutrophic conditions in surface water, based on nutrient concentrations, existed March-May, at about the same time or shortly after ricefields were drained. Secondary Maximum Contaminant Levels established by the U.S. Environmental Protection Agency (USEPA) were exceeded for sulfate, chloride, iron, or manganese in samples from 20 wells, and for iron or manganese in samples from all surface-water sites. Fewer pesticides were detected in ground water than in surface water. In 11 of of the 29 wells sampled, at least one pesticide or pesticide degradation product was detected. The most frequently detected pesticides or pesticide degradation products in ground water were the herbicides benzaton and atrazine. Concentrations of 47 pesticides and degradation products were detected in surface water. At least 3 pesticides were detected in all surface-water samples. In 72 percent of the samples at least 5 hydrophilic pesticides were detected, and in more than 70 percent of the samples at least 3 hydrophobic pesticides were detected. Although atrazine concentrations in three samples collected in the spring exceeded 3 micrograms per liter, the USEPA Maximum Contaminant Level of 3 micrograms per liter was not exceeded because it is based on an annual average of quarterly samples. Concentrations larger than 3.0 micrograms per liter were not detected in samples collected during other times of the year. Tebuthiuron was detected at all surface-water sites; the largest concentration (6.33 micrograms per liter) was detected at a site on Bayou des Cannes, and was the only detection that exceeded the criterion (1.6 micrograms per liter) for the protection of aquatic life. Malathion was detected at 16 surface-water sites; the largest concentration (0.113 micrograms per liter) was detected at a site on Bayou Lacassine and was the only detection that exceeded the criterion (0.1 micrograms per liter) for the protection of aquatic life. Concentrations of fipronil exceeded numeric targets for acute total maximum daily loads (2.3 micrograms per liter) at 3 sites and chronic total maximum daily loads (4.6 micrograms per liter) at 14 sites. Maximum pesticide concentrations in surface water usually occurred in the spring at about the same time or shortly after ricefields were drained. Concentrations of DDE in bed sediment at two sites exceeded interim freshwater sediment quality guidelines for the protection of aquatic life. Fipronil sulfide and desulfinylpronil were detected at all 17 sites from which bed-sediment samples were collected, but there are no current (2002) guidelines with which to evaluate the environmental effects of fipronil and degradation products. Two methods were used to group the ecological data-collection sites: (1) Sites were grouped before data collection (according to the study design) using drainage area

Water-Resources Investigations Report↗

From top to bottom: Do Lake Trout diversify along a depth gradient in Great Bear Lake, NT, Canada?

Depth is usually considered the main driver of Lake Trout intraspecific diversity across lakes in North America. Given that Great Bear Lake is one of the largest and deepest freshwater systems in North America, we predicted that Lake Trout intraspecific diversity to be organized along a depth axis within this system. Thus, we investigated whether a deep-water morph of Lake Trout co-existed with four shallow-water morphs previously described in Great Bear Lake. Morphology, neutral genetic variation, isotopic niches, and life-history traits of Lake Trout across depths (0–150 m) were compared among morphs. Due to the propensity of Lake Trout with high levels of morphological diversity to occupy multiple habitat niches, a novel multivariate grouping method using a suite of composite variables was applied in addition to two other commonly used grouping methods to classify individuals. Depth alone did not explain Lake Trout diversity in Great Bear Lake; a distinct fifth deep-water morph was not found. Rather, Lake Trout diversity followed an ecological continuum, with some evidence for adaptation to local conditions in deep-water habitat. Overall, trout caught from deep-water showed low levels of genetic and phenotypic differentiation from shallow-water trout, and displayed higher lipid content (C:N ratio) and occupied a higher trophic level that suggested an potential increase of piscivory (including cannibalism) than the previously described four morphs. Why phenotypic divergence between shallow- and deep-water Lake Trout was low is unknown, especially when the potential for phenotypic variation should be high in deep and large Great Bear Lake. Given that variation in complexity of freshwater environments has dramatic consequences for divergence, variation in the complexity in Great Bear Lake (i.e., shallow being more complex than deep), may explain the observed dichotomy in the expression of intraspecific phenotypic diversity between shallow- vs. deep-water habitats. The ambiguity surrounding mechanisms driving divergence of Lake Trout in Great Bear Lake should be seen as reflective of the highly variable nature of ecological opportunity and divergent natural selection itself.

Northwest Territories↗