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Fishes of Missouri River, chute, and flood plain habitats: Chapter 4 in Initial biotic survey of Lisbon Bottom, Big Muddy National Fish and Wildlife Refuge

The Lisbon Bottom Unit of the Big Muddy National Fish and Wildlife Refuge (Refuge) is approximately 2,200 acres and is the first complete unit of the Refuge. Primary objectives of the Refuge are to create and restore diverse riverine aquatic habitats and reconnect the Missouri River to its flood plain where feasible. Management seeks to accomplish these objectives by encouraging natural processes of erosion, deposition, and succession to the greatest extent possible. One of the most salient aquatic features of the Lisbon Bottom Unit is a newly created 2-mile-long free-flowing chute, or side channel (Fig. 1). This chute began forming as a levee breech scour hole during the Great Flood of 1993. The chute continued to develop during the 1995 flood and finally cut through to a flowing side channel during the 1996 flood. Extensive erosion and bank sluffing continued during 1997 due to sustained high flows that occurred throughout most of the year. The chute has progressively become wider and deeper with a developing meander pattern and channel bars have begun to form. Lisbon Bottom also contains several seasonal and permanent wetlands and is subject to periodic flooding at high Missouri River stages. Eight studies have been completed or are ongoing to evaluate Missouri River fishes associated with various habitat components of Lisbon Bottom and adjacent Missouri River reaches (Table 1). Several are part of much larger investigations to evaluate fish use of flood-created habitat features, basinwide fish assessment, and endangered or candidate species concerns. At the Lisbon Bottom Unit or in the Missouri River adjacent to the unit 54 fish species were collected (Table 2). Eight of these species have either a protected status under State or Federal laws or biologists consider them to potentially qualify for protected status. The status of the following fish is listed in Table 2: pallid sturgeon x shovelnose sturgeon hybrid, paddlefish, northern pike, sturgeon chub, sicklefin chub, ghost shiner, western silvery minnow, plains minnow, and blue sucker. Equally important to note are fish species that were not collected. The flathead chub, currently listed as State endangered and proposed for Federal listing, were not collected in any of the studies. Common fish species, collected in all seven sampling studies, included gizzard shad, common carp, river carpsucker, and freshwater drum. Paddlefish, skipjack herring, silver carp, white sucker, redfin shiner, western silvery minnow, bullhead minnow, and black bullhead were each collected in only one of the seven studies. Pflieger (1971) developed a guild system for the fishes of Missouri based on distribution patterns and centers of abundance. Fish were assigned to one of four primary faunal groups: Ozark, Big River, Lowland, and Prairie. Two secondary groups (Ozark-prairie and Ozark-lowland) were defined for species equally abundant and distributed in two of the primary areas. Two Ozark-prairie species, shorthead redhorse and white sucker, were collected on or near the Lisbon Bottom Unit. Big River species are found primarily in the Missouri and Mississippi Rivers. The environmental factors controlling fish distribution in these rivers appear to be substrate, current velocity, and turbidity. On or near the Lisbon Bottom Unit 18 Big River species were collected (Table 2). Lowland species are intolerant of siltation and high turbidity. They inhabit standing or slow-moving water with sand, fine gravel, and organic debris substrates. Two Lowland species, bullhead minnow and mosquitofish, were collected in connected scours on the Lisbon Bottom Unit. Prairie species have broader ecological tolerances than the Ozark and Lowland species. They are largely absent in high gradient streams and cool, clear waters. Prairie species make up a significant proportion of Missouri and Mississippi River fishes. Seven Prairie species were collected on or near the Lisbon Bottom Unit (Table 2). The last group, Wide-ranging, was defined as the species more widespread than the other faunal groups with broader environmental tolerances. Most Wide-ranging species occur at least occasionally in all sections of the state. Nineteen Wide-ranging species were collected on or near the Lisbon Bottom Unit (Table 2). Kubisiak (1997) found that isolated scours in the Lower Missouri River were dominated by Wide-ranging species. Connected scours also held Wideranging species but they contained Prairie species and were dominated by Big River species. Faunal group diversity and species richness were compared between isolated (Study l) and connected (Studies 4, 5, 7, and 8) scours of the Lisbon Bottom Unit. Species richness was similar with 22 species collected in isolated scours and an average of 24.8 species collected in connected scours. Isolated scour fish catches were dominated by Wide-ranging fish (59%) with 14% Big River fishes and 14% Prairie fishes. Connected scours had a larger percentage of Big River fish (32%) and fewer Wide-ranging (36%) and Prairie (13%) species than the isolated scours. In the combined studies, Wide-ranging fish dominated the connected scours. A similar comparison was made of fish from chute habitats and the Missouri River. Chute habitats include the Lisbon Bottom Chute, the chute between the right bank and island located at river mile 219, and a shallow channel running between a large sandbar complex and the channel border adjacent to Lisbon Bottom. Species richness in the chutes (35 species) was greater than that of the adjacent river (23 species). Big River species (40%) dominated the chute samples, followed by Wide-ranging at 31% Cand Prairie Cut 14%. The river samples contained equal numbers of Wide-ranging species (35%) and Big River species (35%) with 17% Prairie species. Differences in fish guild diversity and species richness may be due to numerous factors including sampling effort and sampling season. River samples were collected only in Study 3 in October; chute samples were collected in July, August, and October. Fish may have been using the chute in July and August to spawn, feed, or escape high river levels. The Lisbon Bottom Chute is a truly unique feature of the Lower Missouri River. It is the first naturally created side channel to develop since the U.S. Army Corps of Engineers tamed the river. The Corps eliminated 89% of the islands in the Missouri River between 1879 and 1954 (Funk and Robinson 1974). The chutes between the islands and the shore were shallower with less current than the main channel. These areas provided diversity to the Missouri River fish habitat, serving as nursery and feeding areas. The Lisbon Bottom Unit of the Big Muddy National Fish and Wildlife Refuge will continue to be studied in an effort to understand the complex role these areas play in river systems.

Missouri↗

Instream flows to assist the recovery of endangered fishes of the upper Colorado River basin

The riverine landscape of the upper Colorado River basin has been extensively modified by dams, diversions, revetments, and water abstractions. These changes, probably coupled with the introduction of many nonnative fishes, have compromised the existence of four of the native fishes (Colorado River squawfish Ptychocheilus lucius, humpback chub Gila cypha, bonytail chub Gila elegans, and razorback sucker Xyrauchen texanus) of the river system. Efforts to recover these endangered fishes have emphasized reregulation of flows to provide better habitat conditions than existed during the last half century, when ranges and abundances of the fishes declined significantly. Contention emerged, however, with regard to the efficacy of methods used by the U.S. Fish and Wildlife Service to justify flow recommendations to protect the endangered fishes. The purpose of this study was to review the science pertaining to the issue of flow provision, to identify critical uncertainties, and to provide recommendations for determining the instream flow needs of the endangered fishes.

Biological Report↗

More than a decade of conservation biology and research in Sonora and Arizona: The endangered Sonoran Tiger Salamander and threatened Chiricahua Leopard Frog

Only two species of amphibians from Arizona are listed under the federal Endangered Species Act. The Sonoran Tiger Salamander was listed as an endangered species without critical habitat in 1997. The Chiricahua Leopard Frog was listed as a threatened species in 2002; critical habitat was designated in 2012. In this review, we summarize conservation and research conducted on these two species with a focus on work in the southeastern Arizona/northeastern Sonora borderlands, but we also touch upon seminal work on the Chiricahua Leopard Frog at Buenos Aires National Wildlife Refuge (BANWR).

Arizona, Sonora↗

Annual grass invasion is transforming the sagebrush biome’s songbird communities

Novel stressors like climate change and biological invasions alter ecological communities, resulting in changes to ecosystem services and biodiversity (that is, ecological transformation). Most ecological transformation research focuses on plants, but animals are likely affected by and plausibly mediate the extent, impact, and pace of transformations. In western North America, where invasive annual grasses are transforming sagebrush-steppe ecosystems, we quantified how transformation in vegetation drives songbird community change. We hypothesized that transformation of vegetation communities via invasion alters wildlife communities by favoring generalists over specialists, albeit with extinction debts that may temporarily obscure transformations’ consequences. Although local-scale songbird diversity increased with grass invasion, we found that this shift was accompanied by ongoing reorganization of songbird communities at larger scales (across the sagebrush biome), driven by heterogeneous impacts of invasion among species and guilds. Through our biome-wide analysis, we were also able to identify high-priority regions for conservation of sensitive songbird species. Our research provides evidence that wildlife communities are transforming alongside vegetation communities and offers insight into the approaches required to quantify nascent community turnover.

western United States↗

When to target control efforts? Using novel GPS telemetry to quantify drivers of invasive Argentine black and white tegu (Salvator merianae) movement

In South Florida, the Argentine black and white tegu ( Salvator merianae ), a large, omnivorous lizard, has become a recent threat to the Everglades ecosystem. The increase in tegu observations, especially near ecologically sensitive areas such as Everglades National Park, makes informed management critical to contain the tegu population. Using Very High Frequency (VHF) and Global Positioning System (GPS) telemetry, we tracked 24 tegus in the Southern Glades Wildlife Management Area and Redland Agricultural Area in Homestead, Florida from March 2016 to November 2018 and March 2021 to January 2022. We used generalized additive models to determine factors that drive tegu movement to inform managers when traps and surveillance plots are most likely to be effective. Our top model included temporal (time of day and time of year), environmental (air temperature, relative humidity, rain, and wind speed from closest weather station), and biological (sex) variables. This model explained 34.6% of the deviance in tegu minimum rate of movement. We determined that tegus were most active between mid-March and mid-April, and tegu minimum rate of movement positively correlates with air temperature (i.e., highest activity during the hottest part of the day in the early afternoon). We observed a slight positive trend between tegu minimum rate of movement and relative humidity, and no clear trend between rate of movement and either rainfall or wind. Our results can inform natural resource management actions to target tegu removal and surveillance during high activity periods to maximize resource use.

Biological Invasions↗

Visualization of Flow Alternatives, Lower Missouri River

Background The U.S. Army Corps of Engineers (COE) 'Missouri River Master Water Control Manual' (Master Manual) review has resulted in consideration of many flow alternatives for managing the water in the river (COE, 2001; 1998a). The purpose of this report is to present flow-management alternative model results in a way that can be easily visualized and understood. This report was updated in October 2001 to focus on the specific flow-management alternatives presented by the COE in the 'Master Manual Revised Draft Environmental Impact Statement' (RDEIS; COE, 2001). The original version (February 2000) is available by clicking here. The COE, U.S. Fish and Wildlife Service (FWS), Missouri River states, and Missouri River basin tribes have been participating in discussions concerning water management of the Missouri River mainstem reservoir system (MRMRS), the Missouri River Bank Stabilization and Navigation Project, and the Kansas River reservoir system since 1986. These discussions include general input to the revision of the Master Manual as well as formal consultation under Section 7 of the Endangered Species Act. In 2000, the FWS issued a Biological Opinion that prescribed changes to reservoir management on the Missouri River that were believed to be necessary to preclude jeopardy to three endangered species, the pallid sturgeon, piping plover, and interior least tern (USFWS, 2000). The combined Missouri River system is large and complex, including many reservoirs, control structures, and free-flowing reaches extending over a broad region. The ability to assess future impacts of altered management scenarios necessarily involves complex, computational models that attempt to integrate physical, chemical, biological, and economic effects. Graphical visualization of the model output is intended to improve understanding of the differences among flow-management alternatives.

Colorado, Iowa, Kansas, Missouri, Montana, Nebrask↗

Final project memorandum: sea-level rise modeling handbook: resource guide for resource managers, engineers, and scientists

Coastal wetlands of the Southeastern United States are undergoing retreat and migration from increasing tidal inundation and saltwater intrusion attributed to climate variability and sea-level rise. Much of the literature describing potential sea-level rise projections and modeling predictions are found in peer-reviewed academic journals or government technical reports largely suited to reading by other Ph.D. scientists who are more familiar or engaged in the climate change debate. Various sea-level rise and coastal wetland models have been developed and applied of different designs and scales of spatial and temporal complexity for predicting habitat and environmental change that have not heretofore been synthesized to aid natural resource managers of their utility and limitations. Training sessions were conducted with Federal land managers with U.S. Fish and Wildlife Service, National Park Service, and NOAA National Estuarine Research Reserves as well as state partners and nongovernmental organizations across the northern Gulf Coast from Florida to Texas to educate and to evaluate user needs and understanding of concepts, data, and modeling tools for projecting sea-level rise and its impact on coastal habitats and wildlife. As a result, this handbook was constructed from these training and feedback sessions with coastal managers and biologists of published decision-support tools and simulation models for sea-level rise and climate change assessments. A simplified tabular context was developed listing the various kinds of decision-support tools and ecological models along with criteria to distinguish the source, scale, and quality of information input and geographic data sets, physical and biological constraints and relationships, datum characteristics of water and land elevation components, utility options for setting sea-level rise and climate change scenarios, and ease or difficulty of storing, displaying, or interpreting model output. The handbook is designed to be a primer to understanding sea-level rise and a practical synthesis of the current state of knowledge and modeling tools as a resource guide for DOl land management needs and facilitating Landscape Conservation Cooperative (LCC) research and conservation initiatives.

Conference Paper↗

A new tool for studying waterfowl immune and metabolic responses: Molecular level analysis using kinome profiling

Here, we describe the design of an Anas‐ specific kinome peptide array that can be used to study the immunometabolic responses of mallard and American black duck to pathogens, contaminants, and environmental stress. The peptide arrays contain 2,642 unique phosphorylate‐able peptide sequences representing 1,900 proteins. These proteins cover a wide array of metabolic and immunological processes, and 758 Gene Ontology Biological processes are statistically significantly represented on the duck peptide array of those 164 contain the term “metabolic” and 25 “immune.” In addition, we conducted a comparison of mallard to American black duck at a genetic and proteomic level. Our results show a significant genomic and proteomic overlap between these two duck species, so that we have designed a cross‐reactive peptide array capable of studying both species. This is the first reported development of a wildlife species‐specific kinome peptide array.

Ecology and Evolution↗

Detailed study of selenium in soil, water, bottom sediment, and biota in the Sun River Irrigation Project, Freezout Lake Wildlife Management Area, and Benton Lake National Wildlife Refuge, west-central Montana, 1990-92

Selenium and other constituents are adversely affecting water quality and creating a potential hazard to wildlife in several areas of the Sun River Irrigation Project, Freezout Lake Wildlife Management Area, and Benton Lake National Wildlife Refuge in west-central Montana. Selenium derived from Cretaceous shale and Tertiary and Quaternary deposits containing shale detritus is transported in the oxic shallow ground-water systems. At Freezout Lake Wildlife Management Area, drainage from irrigated glacial deposits is the primary source of selenium; drainage from non-irrigated farmland is a significant source locally. Benton Lake generally receives more selenium from natural runoff from its non-irrigated basin than from the trans-basin diversion of irrigation return flow. Selenium has accumulated in aquatic plants and invertebrates, fish, and water birds, particularly in wetlands that receive the largest selenium loads. Although selenium residues in biological tissue from some wetland units exceeded biological risk levels, water-bird reproduction generally has not been impaired. The highest selenium residues in biota commonly occurred in samples from Priest Butte Lakes, which also had the highest selenium concentration in wetland water. Selenium concentrations in all invertebrate samples from Priest Butte Lakes and the south end of Freezeout Lake exceeded the critical dietary threshold for water birds. Selenium delivered to wetlands accumulates in bottom sediment, predominantly in near-shore areas. Potential impacts to water quality, and presumably biota, may be greatest near the mouths of inflows. Most selenium delivered to wetlands will continue to accumulate in bottom sediment and biota.

Water-Resources Investigations Report↗

Recent population size, trends, and limiting factors for the double-crested Cormorant in Western North America

The status of the double-crested cormorant ( Phalacrocorax auritus ) in western North America was last evaluated during 1987–2003. In the interim, concern has grown over the potential impact of predation by double-crested cormorants on juvenile salmonids ( Oncorhynchus spp.), particularly in the Columbia Basin and along the Pacific coast where some salmonids are listed for protection under the United States Endangered Species Act. Recent re-evaluations of double-crested cormorant management at the local, flyway, and federal level warrant further examination of the current population size and trends in western North America. We collected colony size data for the western population (British Columbia, Washington, Oregon, Idaho, California, Nevada, Utah, Arizona, and the portions of Montana, Wyoming, Colorado and New Mexico west of the Continental Divide) by conducting aircraft-, boat-, or ground-based surveys and by cooperating with government agencies, universities, and non-profit organizations. In 2009, we estimated approximately 31,200 breeding pairs in the western population. We estimated that cormorant numbers in the Pacific Region (British Columbia, Washington, Oregon, and California) increased 72% from 1987–1992 to circa 2009. Based on the best available data for this period, the average annual growth rate (λ) of the number of breeding birds in the Pacific Region was 1.03, versus 1.07 for the population east of the Continental Divide during recent decades. Most of the increase in the Pacific Region can be attributed to an increase in the size of the nesting colony on East Sand Island in the Columbia River estuary, which accounts for about 39% of all breeding pairs in the western population and is the largest known breeding colony for the species (12,087 breeding pairs estimated in 2009). In contrast, numbers of breeding pairs estimated in coastal British Columbia and Washington have declined by approximately 66% during this same period. Disturbance at breeding colonies by bald eagles ( Haliaeetus leucocephalus ) and humans are likely limiting factors on the growth of the western population at present. Because of differences in biology and management, the western population of double-crested cormorants warrants consideration as a separate management unit from the population east of the Continental Divide.

Journal of Wildlife Management↗

Anticoagulant rodenticides in Strix owls indicate widespread exposure in west coast forests

Exposure of nontarget wildlife to anticoagulant rodenticides (AR) is a global conservation concern typically centered around urban or agricultural areas. Recently, however, the illegal use of ARs in remote forests of California, USA, has exposed sensitive predators, including the federally threatened northern spotted owl ( Strix occidentalis caurina ). We used congeneric barred owls ( S. varia ) as a sentinel species to investigate whether ARs pose a threat to spotted owls and other old-forest wildlife in northern regions of the Pacific Northwest. We analyzed the liver tissue from 40 barred owls collected in Oregon and Washington and confirmed exposure to ≥1 AR compounds in 48% of the owls examined. Brodifacoum, an extremely toxic second-generation AR, was the most common compound detected (89% of positive cases), followed by bromadiolone (11%), difethialone (11%), and warfarin (5%). Brodifacoum was also detected in one barred owl and one spotted owl opportunistically found dead (liver concentrations were 0.091 and 0.049 µg/g, respectively). We found no evidence that exposure varied with proximity to developed and agricultural areas, or among different study areas, age-classes, and sexes. Rather, exposure was ubiquitous, and the rates we observed in our study (38 – 64%) were similar to or greater than that reported previously for barred owls in California (40%). Together these studies indicate widespread contamination in forested landscapes used by spotted owls and other wildlife of conservation concern. Owls collected in older forests may have been exposed via illegal use of ARs, highlighting a mounting challenge for land managers and policy makers.

California↗

The geographic extent of bird populations affected by renewable-energy development

Bird populations are declining globally. Wind and solar energy can reduce emissions of fossil fuels that drive anthropogenic climate change, yet renewable-energy production represents a potential threat to bird species. Surveys to assess potential effects at renewable-energy facilities are exclusively local, and the geographic extent encompassed by birds killed at these facilities is largely unknown, which creates challenges for minimizing and mitigating the population-level and cumulative effects of these fatalities. We performed geospatial analyses of stable hydrogen isotope data obtained from feathers of 871 individuals of 24 bird species found dead at solar- and wind-energy facilities in California (USA). Most species had individuals with a mix of origins, ranging from 23% to 98% nonlocal. Mean minimum distances to areas of likely origin for nonlocal individuals were as close as 97 to >1250 km, and these minimum distances were larger for species found at solar-energy facilities in deserts than at wind-energy facilities in grasslands (Cohen's d = 6.5). Fatalities were drawn from an estimated 30–100% of species’ desingated ranges, and this percentage was significantly smaller for species with large ranges found at wind facilities (Pearson's r = −0.67). Temporal patterns in the geographic origin of fatalities suggested that migratory movements and nonmigratory movements, such as dispersal and nomadism, influence exposure to fatality risk for these birds. Our results illustrate the power of using stable isotope data to assess the geographic extent of renewable-energy fatalities on birds. As the buildout of renewable-energy facilities continues, accurate assessment of the geographic footprint of wildlife fatalities can be used to inform compensatory mitigation for their population-level and cumulative effects.

Conservation Biology↗

Refuge management analyses: levee alternatives at Clarence Cannon National Wildlife Refuge

This report documents the results of a workshop that examined levee alternatives at Clarence Cannon National Wildlife Refuge. This refuge is located along the Mississippi River, approximately 50 miles north of St. Louis. It was purchased primarily for waterfowl maintenance. A levee providing flood protection from the Mississippi River has broken four times since the Refuge was acquired in 1965. A number of alternatives have been proposed in response to the most recent break in 1983. Long term alternatives considered at the workshop included: Improve the existing levee by raising the height approximately 2 ft. Improve the existing levee by raising the height approximately 2 ft. Maintain the levee at a lower elevation by including a spillway about 3 ft lower than the existing levee height. Do not maintain a levee so that the Refuge will flood directly with river stage. Repair of the major breaks in the levee, but not the more general wave damage, was considered as a short term alternative. Participants first specified the habitats and management controls desired at Clarence Cannon National Wildlife Refuge. These were centered around attaining the maximum feasible development and management of moist soil units. Levee alternatives were evaluated in terms of their ability to provide the desired habitats and management controls. Preliminary cost estimates were prepared for each alternative, and the qualitative consequences of each alternative identified for the full set of outputs from the Refuge Master Plan. The alternative of improving the existing levee by raising the height was unanimously preferred on purely “biological” grounds (with cost not considered). Repairing the levee with no change in elevation was unanimously selected as the most cost effective alternative.

Missouri↗

Rainfall reduces the potential for competitive suppression of a globally endangered ungulate by livestock

Protected areas often are too small to house populations of wide-ranging species. Viability of wildlife populations therefore depends on whether interactions with humans and their land uses are negative, neutral, or positive. In central Iran, we measured interactions between globally endangered onagers (Equus hemionus onager) and livestock by analyzing remotely-sensed vegetation metrics within livestock grazing areas, tracking 9 animals with GPS telemetry, and assessing onagers' diet quality through analysis of fecal samples. Resource selection by onagers depended both on season and the presence of livestock. During the dry season, livestock reduced forage (some combination of forage biomass and forage quality) compared to pre-grazing periods, demonstrating potential for competitive suppression of onagers by livestock when resources are scarce. Additionally, and during both seasons, selection for forage by onagers was accentuated at night when livestock were absent, indicating onager avoidance of livestock. During the wet season, onagers exposed to livestock exhibited higher-quality diets than those that did not co-occur with livestock, suggesting that livestock grazing may potentially enhance forage quality for onagers. Consequently, collaboration with pastoralists to regularly rotate the locations of dry and wet season leases could alleviate negative effects of livestock grazing on onagers. Similar to other cases in multi-use landscapes, temporal shifts in the strength of competition—driven by diel cycles and seasonal rainfall—may characterize wildlife-livestock interactions in Iran and elsewhere in Asian rangelands. Our study highlights the possibility that conservation of an endangered mammal could be compatible with livestock production, at least during wet seasons.

Bahram-e-Goor Protected Area (BPA), Qatrouiyeh Nat↗

Occurrence of organochlorine pesticides in streambed sediment and fish from selected streams on the island of Oahu, Hawaii, 1998

Organochlorine pesticides were heavily used from the mid-1940s to the mid-1980s. The persistence of organochlorine pesticides, their tendency to accumulate in soil, sediment, and biota, and their harmful effects on wildlife brought this class of compounds into disfavor and eventually resulted in restriction or cancellation of most of them in the United States (Nowell and others, 1999). Despite use restrictions, these compounds continue to be detected in sediment and fish tissue samples. This study was undertaken as part of the National Water-Quality Assessment (NAWQA) program of the U.S. Geological Survey (USGS). The NAWQA program assesses watersheds as integrated systems, focusing on chemical concentrations, physical conditions, and biological status in streams. One component of NAWQA is an occurrence survey of organic contaminants and trace elements in stream bed sediment and fish tissue. The goal of the Oahu stream bed sediment and fish tissue occurrence survey was to determine which organochlorine contaminants are present in streams around the island, and with which land uses they are associated. An understanding of relations between land use and organochlorine compounds will allow land management practices to be designed to reduce the loading of contaminants to streams and nearshore waters.

Hawaii↗

Wind River subbasin restoration: Annual report of U.S. Geological Survey activities January 2023 through December 2023

We sampled juvenile wild Oncorhynchus mykiss (Steelhead Trout) in headwater streams of the Wind River, WA, to characterize population attributes and investigate life-history metrics, particularly migratory patterns, and early life-stage survival. We used passive integrated transponder (PIT) tagging and a series of instream PIT-tag interrogation systems (PTISs) to track juveniles and adults. The Wind River subbasin is considered a wild Steelhead refuge by Washington Department of Fish and Wildlife (WDFW). No hatchery Steelhead Trout have been released in the Wind River subbasin since 1997, and hatchery adults are estimated at less than one percent of spawners in most years. Over twenty years of Steelhead Trout status and trend monitoring and research in the subbasin is contributing to understanding of population response to numerous restoration actions in the subbasin, including removal of Hemlock Dam from Trout Creek in 2009, which had an inadequate adult ladder and contributed to increased water temperatures. Data from our study, and companion work by Washington Department of Fish and Wildlife, are contributing to the Columbia Basin Fish and Wildlife Program (2008) Research, Monitoring, and Evaluation (RM&E) Strategy of Fish Population Status Monitoring. Specifically, this work addresses the sub-strategies of 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and Uncertainties Research regarding differing life histories of a wild Steelhead Trout population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. During summer and fall 2023, we PIT-tagged 1,294 Steelhead Trout parr (age-0 and age-1), in the Trout Creek and upper Wind River watersheds. Age-0 parr were at high abundance due to a strong spawning run in 2023 (estimate of 814 adults from September snorkel survey compared to 22-year median of 450; Charlie Cochran, WDFW Fish Biologist, personal commun., 2022), but age-1 parr abundance was low following poor spawner numbers in 2022 (estimate of 159 adults from September snorkel survey). An additional 189 age-2 or older parr were tagged to provide fish for estimating detection efficiencies at PTISs. Steelhead Trout parr were recaptured and detected through repeat headwater sampling, smolt trapping, instream PTISs and Columbia River PIT-tag detection. We maintained and upgraded six instream PTISs to detect PIT-tagged Steelhead Trout parr, smolts, and adults, providing data for population assessments, and life-cycle research. Detection data from PIT-tagged adult Steelhead Trout at PTISs allow assessment of adult escapement to tributary watersheds within the Wind River subbasin. Adult Steelhead Trout detection efficiency estimates at our primary PTIS in Trout Creek have been greater than 99 percent during seven of the past nine years and have exceeded 97% at our primary PTIS in the Wind River during eight of the past nine years. Adult escapement estimates to tributary watersheds are helping evaluate the efficacy of the 2009 removal of Hemlock Dam from rkm 2.0 of Trout Creek, where it had potential negative effects on Steelhead Trout populations due to hydrologic impairment, increased temperatures, and adult passage issues because of an inadequate fish ladder. Detections at the instream PTISs have shown trends of age-0 and age-1 Steelhead Trout parr emigration from natal areas during summer and fall, in addition to the expected movement of parr and smolts in spring. Our data suggest that often most fish from a cohort that migrate downstream will do so at age-1 for additional rearing downstream of their natal areas. It is unknown if this is ingrained behavior or a result of lack of habitat capacity. We have estimated that from 15 to 56% of parr tagged as age-0 fish in headwater areas make downstream migrations at age-1 for additional rearing. We have estimated that up to 27% of Steelhead Trout parr, tagged as age-1 fish, make downstream migrations during fall: this is especially pronounced in the upper Wind River portion of the watershed. These findings raise questions about preferred parr rearing habitat and whether migrations are density- or habitat-quality driven, and answers to such questions are part of the long-term goals of this study as active and passive habitat restoration actions occur. Repeat sampling at sites in the subbasin within and between years has enabled assessment of juvenile Steelhead Trout growth patterns. Growth rates (relative change in weight) of age-0 PIT-tagged parr during summer have been similar across the subbasin, though slightly lower in the Trout Creek watershed. Summer growth rates have been lower for age-1 parr in the Trout Creek watershed than the upper Wind River watershed. Yearly relative growth was similar across the subbasin for both age-0 and age-1 tagged parr. Non-native Salvelinus fontinalis (Brook Trout) are present in the subbasin, chiefly the Trout Creek watershed, and repeat sampling provides an index of their prevalence. Mean percent-of-catch that is Brook Trout, at four sample sites in Trout Creek, has declined from the period 1998 – 2003 to the period 2011 – 2024. Percent-of-catch and number of Brook Trout at the Trout Creek sites from 2011 through 2022, though variable, have generally declined. Evaluation and planning of habitat restoration efforts are critical to ensure efficient use of money and resources. Assessing Steelhead Trout life history variation in the Wind River subbasin informs research and tracking of many populations and habitat restoration and water allocation planning. Movement of Steelhead Trout parr from natal areas to other rearing areas raises questions regarding juvenile abundance, origin, and habitat use within watersheds. Improved PTISs and focused PIT-tagging of age-0 and age-1 Steelhead Trout parr allow investigation of such questions. Detailed viable salmonid population and life-history data, such as that provided by PIT-tagging and instream PTIS networks inform fisheries policy and management and enable assessment of long-term effects of habitat restoration actions such as the removal of Hemlock Dam on Trout Creek and proposed major instream habitat restoration in the upper Wind River.

Washington↗

Wind River subbasin restoration: Annual report of U.S. Geological Survey activities January 2024 through December 2024

We sampled juvenile wild Oncorhynchus mykiss (Steelhead Trout) in headwater streams of the Wind River, Washington, to characterize population attributes and investigate life-history metrics, particularly migratory patterns, and early life-stage survival. Additionally, this sampling provides an index of abundance of non-native Brook Trout Salvelinus confluentus . We used passive integrated transponder (PIT) tagging and a series of instream PIT-tag interrogation systems (PTISs) to track juveniles and adults. The Wind River subbasin is considered a wild Steelhead refuge by Washington Department of Fish and Wildlife (WDFW). No hatchery Steelhead Trout have been released in the Wind River subbasin since 1997, and hatchery adults are estimated at less than one percent of spawners in most years. Over 25 years of Steelhead Trout status and trend monitoring and research in the subbasin is contributing to understanding of population response to numerous restoration actions in the subbasin, including removal of Hemlock Dam from Trout Creek in 2009, which had an inadequate adult ladder and contributed to increased water temperatures, to near lethal levels for salmonids. Data from our study, and companion work by WDFW, are contributing to the Columbia Basin Fish and Wildlife Program (2009) Research, Monitoring, and Evaluation (RM&E) Strategy of Fish Population Status Monitoring. Specifically, this work addresses the sub-strategies of 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and Uncertainties Research regarding differing life histories of a wild Steelhead Trout population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. During summer and fall 2024, we PIT-tagged 2,075 Steelhead Trout parr (age-0 and age-1), in the Trout Creek and upper Wind River watersheds. Age-0 and age-1 parr were at high abundance due to strong spawner numbers in 2023 and 2024 (Estimate of 932 in 2023; and a moderate number of spawners in 2024 though an estimate is not available at this time; 24-year median = 619; Charlie Cochran, WDFW Fish Biologist, personal commun., 2025). An additional 100 parr (age-0 and age-2 or older) were tagged to provide fish for estimating detection efficiencies at PTISs. Steelhead Trout parr were recaptured and detected through repeat headwater sampling, smolt trapping, instream PTISs and Columbia River mainstem PIT-tag detection. We maintained and upgraded six instream PTISs to detect PIT-tagged Steelhead Trout parr, smolts, and adults, providing data for population assessments, and life-cycle research. Detection data from PIT-tagged adult Steelhead Trout at our four primary PTISs allow assessment of adult escapement to tributary watersheds within the Wind River subbasin. Most adult Steelhead detected were tagged by WDFW at an adult trap in the lower Wind River and some as smolts at screw traps. Adult Steelhead Trout detection efficiency estimates at our primary PTIS in Trout Creek have been greater than 99 percent during eight of the past ten years and have exceeded 97% at our primary PTIS in the Wind River during nine of the past ten years. Adult escapement estimates to tributary watersheds are helping evaluate the efficacy of the 2009 removal of Hemlock Dam from rkm 2.0 of Trout Creek. Detections at the instream PTISs have shown trends of age-0 and age-1 Steelhead Trout parr emigration from natal areas during summer and fall, in addition to the expected movement of parr and smolts in spring. Our data suggest that most fish from a cohort that migrate downstream will do so at age-1 for additional rearing in the subbasin. We have estimated that from 15% to 56% of parr tagged as age-0 fish in headwater areas make downstream migrations at age-1 for additional rearing (median = 29%). We have estimated that from 1% to 27% of Steelhead Trout parr, tagged as age-1 fish, make downstream migrations during fall (median = 3.5%): this is especially pronounced in the upper Wind River portion of the watershed (median 10%). These findings raise questions about parr rearing habitat and whether migrations are density- or habitat-quality driven, and answers to such questions are long-term goals of this study as active and passive habitat restoration occurs. Repeat sampling at sites in the subbasin within and between years has enabled assessment of juvenile Steelhead Trout growth patterns. Growth rates (relative change in weight) of age-0 PIT-tagged parr during summer have been similar across the subbasin, though slightly lower in the Trout Creek watershed (Trout Creek sites, 0.57 - 1.25%/day, Wind River sites = 0.94 – 2.48%/day). Summer growth rates have been lower for age-1 parr in the Trout Creek watershed than the upper Wind River watershed (Trout Creek sites, = 0.03 - 0.14%/day, Wind River sites = 0.05 – 0.19%/day). Yearly relative growth was similar across the subbasin for both age-0 and age-1 tagged parr. During 2024 range of mean daily relative change in weight from the previous year for age-0 tagged fish was: Trout Creek sites = 0.96 – 1.41%/day, Wind River sites = 0.80 – 1.30 %/day; and for age1 tagged fish was: Trout Creek sites = 0.37 - 0.50 %/day, Wind River sites = no age-1 tagged recaptures. Non-native Brook Trout are present in the subbasin, chiefly the Trout Creek watershed. Mean percent-of-catch that is Brook Trout, at four sample sites in Trout Creek, has declined from the period 1998 – 2003 to the period 2011 – 2024 (range of decrease = 19 - 82%). Percent-of-catch (of 64 samples, 52 < 10%; 3 > 20%) and number of Brook Trout at the Trout Creek sites from 2011 through 2024, though variable, have generally declined. Habitat restoration evaluation efforts are critical to ensure efficient use of money and resources but require long-term monitoring of population metrics. Detailed viable salmonid population and life-history data, such as that provided by PIT-tagging and instream PTIS networks inform fisheries policy and management and enable assessment of long-term effects of habitat restoration actions such as the removal of Hemlock Dam on Trout Creek and proposed major instream habitat restoration in the upper Wind River.

Washington↗

Spatially explicit decision support for selecting translocation areas for Mojave desert tortoises

Spatially explicit decision support systems are assuming an increasing role in natural resource and conservation management. In order for these systems to be successful, however, they must address real-world management problems with input from both the scientific and management communities. The National Training Center at Fort Irwin, California, has expanded its training area, encroaching U.S. Fish and Wildlife Service critical habitat set aside for the Mojave desert tortoise ( Gopherus agassizii ), a federally threatened species. Of all the mitigation measures proposed to offset expansion, the most challenging to implement was the selection of areas most feasible for tortoise translocation. We developed an objective, open, scientifically defensible spatially explicit decision support system to evaluate translocation potential within the Western Mojave Recovery Unit for tortoise populations under imminent threat from military expansion. Using up to a total of 10 biological, anthropogenic, and/or logistical criteria, seven alternative translocation scenarios were developed. The final translocation model was a consensus model between the seven scenarios. Within the final model, six potential translocation areas were identified.

Biodiversity and Conservation↗