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At least 613 records · Page 34Linked to original sources
USGS2024

Quantifying fine sediment infiltration in spawning gravel used by Chinook salmon (Oncorhynchus tshawytscha) in the Sauk River Basin, Washington, 2018–21

Fine sediment can infiltrate into river substrate that salmonid fish species ( Oncorhynchus spp.) use to spawn. High levels of sediment infiltration can increase egg-to-fry mortality, which corresponds to the period when salmonids are still residing in the subsurface gravels. This study quantifies fine sediment infiltration of Chinook salmon ( Oncorhynchus tshawytscha ) spawning habitat during the egg-to-fry emergence period over three years in the Sauk River, which has naturally high fine sediment loads and important native salmon populations. Additionally, this study qualitatively assesses how grain size distribution of the riverbed and adjacent gravel bars compare to grain size distribution following fine sediment infiltration to evaluate if riverbed or gravel bar grain size distributions may provide information on the potential for fine sediment infiltration in spawning gravels. Fine sediment infiltration into spawning gravels was quantified using sediment boxes and infiltration bags that were installed in artificial redds constructed at known Chinook salmon spawning locations at three study sites on the Sauk River over the expected egg-to-fry period. Over the three-year study period (August 2018–April 2021), fraction finer of sediment (grain sizes of less than two millimeters), ranged from 0.12 to 0.23 across the three study sites and years. Based on a comparison of field observations from this study and percentage egg-to-fry survival curves found in the literature, the expected survival for Chinook salmon eggs in the Sauk River is roughly 30 percent. Expected survival increases to approximately 90 percent if eggs are eyed and thus farther along in their development. Our field study did not evaluate the progression of infiltration, so it is unknown if observed fine sediment infiltration was at this relatively high rate during the period that corresponded to early egg development, when eggs are more sensitive to fine sediment infiltration. Dissolved oxygen in the gravels is largely above critically low levels (4 milligrams per liter) during sensitive periods corresponding to egg development and is interpreted not to affect egg-to-fry mortality. Active channel morphology in the middle reaches of the Sauk River may pose an additional challenge to pre-emergence survival. Channel change, deposition, and potential scour at the middle Sauk River study site likely contributed to low recovery rates of both sediment boxes and infiltration bags in two of the three study years. In terms of grain size distributions of riverbed sediment and adjacent gravel bars representing the potential for fine sediment infiltration into spawning gravels, both riverbed and gravel bar bulk subsurface sediment samples had higher fraction finer for the representative fine grain size of two millimeters compared to the sediment boxes and infiltration bags. Therefore, riverbed and gravel bar samples may serve as a conservative first order proxy for potential fine sediment infiltration into spawning gravels, with the understanding that these samples may overestimate fine sediment infiltration by up to 15 percent.

Washington↗
USGS2002

A statistical model for estimating stream temperatures in the Salmon and Clearwater River basins, central Idaho

A water-quality standard for temperature is critical for the protection of threatened and endangered salmonids, which need cold, clean water to sustain life. The Idaho Department of Environmental Quality has established temperature standards to protect salmonids, yet little is known about the normal range of temperatures of most Idaho streams. A single temperature standard for all streams does not take into account the natural temperature variation of streams or the existence of naturally warm waters. To address these issues and to help the Idaho Department of Environmental Quality revise the existing State temperature standards for aquatic life, temperature data from more than 200 streams and rivers in the salmon and Clearwater River Basins were collected. From these data, a statistical model was developed for estimating stream temperatures on the basis of subbasin and site characteristics and climatic factors. Stream temperatures were monitored hourly for approximately 58 days during July, August, and September 2000 at relatively undisturbed sites in subbasins in the Salmon and Clearwater River Basins in central Idaho. The monitored subbasins vary widely in size, elevation, drainage area, vegetation cover, and other characteristics. The resulting data were analyzed for statistical correlations with subbasin and site characteristics to establish the most important factors affecting stream temperature. Maximum daily average stream temperatures were strongly correlated with elevation and total upstream drainage area; weaker correlations were noted with stream depth and width and aver-age subbasin slope. Stream temperatures also were correlated with certain types of vegetation cover, but these variables were not significant in the final model. The model takes into account seasonal temperature fluctuations, site elevation, total drainage area, average subbasin slope, and the deviation of daily average air temperature from a 30-year normal daily average air temperature. The goodness-of-fit of the model varies with day of the year. Overall, temperatures can be estimated with 95-percent confidence to within approximately plus or minus 4 degrees Celsius. The model performed well when tested on independent stream-temperature data previously collected by the U.S. Geological Survey and other agencies. Although the model provides insight into the natural temperature potential of a wide variety of streams and rivers in the Salmon and Clearwater River Basins, it has limitations. It is based on data collected in only one summer, during which temperatures were higher and streamflows were lower than normal. The effects of changes in streamflow on the effectiveness of the model are not known. Because the model is based on data from minimally disturbed or undisturbed streams, it should not be applied to streams known to be significantly affected by human activities such as disturbance of the streambed, diversion and return of water by irrigation ditches, and removal of riparian vegetation. Finally, because the model is based on data from streams in the Salmon and Clearwater River Basins and reflects climatological and landscape characteristics of those basins, it should not be applied to streams outside this region.

Idaho↗
USGS1997

Summary of information on aquatic biota and their habitats in the Willamette Basin, Oregon, through 1995

Available information on aquatic biota of the Willamette Basin was reviewed and summarized to describe current and historical conditions as part of the U.S. Geological Survey's National Water-Quality Assessment Program. Biological parameters emphasized include the status, distribution, and trends of aquatic biota, particularly algae, macroinvertebrates, and fish; the condition of aquatic and riparian habitat in which these biota reside; and the response of these biota to natural and human-associated impacts, including the level, type, and effect of contaminants. Considerable data are available on aquatic biota in the Willamette Basin, although the information is highly uneven relative to taxa and spatial scope. Extensive information exists for high-profile taxa, such as salmonid fishes, but less information is available for macroinvertebrates, and relatively little data exist for algae. Additionally, some areas such as the H.J. Andrews Experimental Forest and the main stem Willamette River have been extensively studied, whereas data are limited for many other areas. The basin supports a diverse aquatic macroinvertebrate fauna. Available data indicate a relatively high diversity of taxa and a high richness of Ephemeroptera, Plecoptera, Trichoptera (EPT) taxa in the upper reaches of the basin. In the lower main stem reaches, macroinvertebrate assemblages are dominated by pollution tolerant organisms and those adapted to low dissolved oxygen levels. Most of the limited data on algae are from sampling in the main stem Willamette River. Diatoms and blue-green algae are the dominant algal forms. Approximately 61 fish species occur in the basin, although nearly half are introduced. Species richness and distribution are highly correlated with elevation, stream gradient, and water temperature. High elevation, cold water, mountain streams are characterized by a few species of salmonids, sculpins, suckers, and whitefish. Low elevation, main stem reaches of major rivers and streams are dominated by warm water species, such as bass, catfish, and several species in the pan fish group. The only species of fish listed as threatened or endangered is the Oregon chub (Oregonichthys crameri). The effect of an expanding human presence in the Willamette Basin has substantially altered aquatic and riparian habitats, and the biota that use or reside in these habitats. Construction of dams, channelization and bank stabilization of rivers, species introductions, supplementations of fisheries through aquaculture, timber harvesting, agricultural activities, and urbanization have contributed to changes in aquatic habitats and biota from historical conditions. Aquatic toxicological investigations in the basin have focused primarily on fish. These studies have addressed chlorinated pesticides, polychlorinated biphenyls (PCBs), dioxins and furans, polycyclic aromatic hydrocarbons (PAHs), and trace elements in aquatic tissue, as well as fish health assessments, skeletal abnormalities, and aquatic toxicological responses. Several pesticides exceeded U.S. Environmental Protection Agency and State water-quality criteria for the protection of aquatic life. Elevated PCB, dioxin, and furan concentrations were associated with point sources, such as pulp and paper mills. Elevated concentrations of mercury in aquatic tissue were associated with several reservoirs. Fish health assessments and skeletal abnormality studies detected high levels of abnormalities in fish from the main stem Willamette River. Few investigations have examined aquatic toxicological responses, such as enzyme induction assays, growth assays, and biomarker studies.

Oregon↗
USGS1995

Development of a DNA probe for the myxosporean parasite, Ceratomyxa shasta, using the polymerase chain reaction with arbitrary primers

The arbitrarily primed polymerase chain reaction (PCR) was used to generate a DNA marker specific for the myxosporean parasite Ceratomyxa shasta . The [ 32 P]-labeled marker hybridized to purified C. shasta DNA and to parasite DNA combined with salmonid DNA in a dot blot assay, demonstrating its potential as a diagnostic tool. The amplified DNA segment was cloned and sequenced, and primers specific for the marker were designed. When these primers were used in a standard PCR assay, DNA was amplified from C. shasta and from infected fish tissues, but not from uninfected fish tissues or from 2 other myxosporean parasites. The sensitivity of the PCR assay will permit detection of low levels of C. shasta from infected fish or oligochaetes and will be useful in defining the parasite's life cycle as well as examining its impact on salmonid populations.

Diseases of Aquatic Organisms↗
USGS1999

Antigenic and functional characterization of p57 produced by Renibacterium salmoninarum

Renibacterium salmoninarum , the causative agent of bacterial kidney disease, produces large quantities of a 57-58 kDa protein (p57) during growth in broth culture and during infection of salmonid fish. Biological activities of secreted p57 include agglutination of salrnonid leucocytes and rabbit erythrocytes. We define the location of epitopes on p57 recognized by agglutination-blocking monoclonal antibodies (MAbs) 4Cl1, 4H8 and 4D3, and demonstrate that the majority of secreted p57 is a nlonomer that retains salrnonid leucocyte agglutinat~ng activity. The 3 MAbs bound a recombinant, amino-terminal fragment of p57 (211 aa) but not a carboxy-terminal fragment (315 aa) demonstrating that the neutralizing epitopes are located within the amino-terminal portion of p57. When combinations of the MAbs were used in an antigen capture ELISA. the epitopes recognized by the 3 MAbs were shown to be sterically separate. However, when the same MAb was used as both the coating and detection MAb, binding of the biotinylated detection MAb was not observed. These data indicate that the epitopes recognized by the 3 agglutination-blocking antibodies are functionally available only once per molecule and that native p57 exists as a monomer Similar ELISA results were obtained when kidney tissues from 3 naturally infected chinook salmon were assayed. Finally, a p57 monomer was purified using anion exchange and size exclusion chromatography that retained in vitro agglutinating activity. A model in which p57 is released from R. salmoninarum as a biologically active monomer during infection of salmonid fish is proposed.

Diseases of Aquatic Organisms↗
USGS2001

Mortality and kidney histopathology of Chinook salmon Oncorhynchus tshawytscha exposed to virulent and attenuated Renibacterium salmoninarum strains

An isolate of Renibacterium salmoninarum (strain MT 239) exhibiting reduced virulence in rainbow trout Oncorhynchus mykiss was tested for its ability to cause bacterial kidney disease (BKD) in chinook salmon Oncorhynchus tshawytscha, a salmonid species more susceptible to BKD. Juvenile chinook salmon were exposed to either 33209, the American Type Culture Collection type strain of R. salmoninarum, or to MT 239, by an intraperitoneal injection of 1 x 10(3) or 1 x 10(6) bacteria fish(-1), or by a 24 h immersion in 1 x 10(5) or 1 x 10(7) bacteria ml(-1). For 22 wk fish were held in 12 degrees C water and monitored for mortality. Fish were sampled periodically for histological examination of kidney tissues. In contrast to fish exposed to the high dose of strain 33209 by either injection or immersion, none of the fish exposed to strain MT 239 by either route exhibited gross clinical signs or histopathological changes indicative of BKD. However, the MT 239 strain was detected by the direct fluorescent antibody technique in 4 fish that died up to 11 wk after the injection challenge and in 5 fish that died up to 20 wk after the immersion challenge. Viable MT 239 was isolated in culture from 3 fish that died up to 13 wk after the immersion challenge. Total mortality in groups injected with the high dose of strain MT 239 (12%) was also significantly lower (p < 0.05) than mortality in groups injected with strain 33209 (73 %). These data indicate that the attenuated virulence observed with MT 239 in rainbow trout also occurs in a salmonid species highly susceptible to BKD. The reasons for the attenuated virulence of MT 239 were not determined but may be related to the reduced levels of the putative virulence protein p57 associated with this strain.

Diseases of Aquatic Organisms↗
USGS2001

PCR-based assays for the fish pathogen Aeromonas salmonicida: Further evaluation and validation of three PCR primer sets with infected fish

Two Aeromonas salmonicida -specific polymerase chain reaction (PCR) tests and 1 A. salmonicida subsp. salmonicida -specific PCR test were used to screen salmonid populations that were either overtly or covertly infected with A. salmonicida subsp. salmonicida . It was demonstrated that these PCR assays could be used to replace the biochemical testing currently employed to confirm the identity of A. salmonicida isolates cultured from infected fish. The AP and PAAS PCR assays were also capable of direct detection of A. salmonicida in overtly infected fish, with mucus, gill and kidney samples most likely to yield a positive result. Culture was a more reliable method for the direct detection of A. salmonicida in covertly infected salmonids than was the direct PCR testing of tissue samples, with the AP and PAAS PCRs having a lower detection limit (LDL) of approximately 4 x 10 5 colony-forming units (CFU) g -1 sample.

Diseases of Aquatic Organisms↗
USGS2003

Two distinct phylogenetic clades of infectious hematopoietic necrosis virus overlap within the Columbia River basin

Infectious hematopoietic necrosis virus (IHNV), an aquatic rhabdovirus, causes a highly lethal disease of salmonid fish in North America. To evaluate the genetic diversity of IHNV from throughout the Columbia River basin, excluding the Hagerman Valley, Idaho, the sequences of a 303 nt region of the glycoprotein gene (mid-G) of 120 virus isolates were determined. Sequence comparisons revealed 30 different sequence types, with a maximum nucleotide diversity of 7.3% (22 mismatches) and an intrapopulational nucleotide diversity of 0.018. This indicates that the genetic diversity of IHNV within the Columbia River basin is 3-fold higher than in Alaska, but 2-fold lower than in the Hagerman Valley, Idaho. Phylogenetic analyses separated the Columbia River basin IHNV isolates into 2 major clades, designated U and M. The 2 clades geographically overlapped within the lower Columbia River basin and in the lower Snake River and tributaries, while the upper Columbia River basin had only U clade and the upper Snake River basin had only M clade virus types. These results suggest that there are co-circulating lineages of IHNV present within specific areas of the Columbia River basin. The epidemiological significance of these findings provided insight into viral traffic patterns exhibited by IHNV in the Columbia River basin, with specific relevance to how the Columbia River basin IHNV types were related to those in the Hagerman Valley. These analyses indicate that there have likely been 2 historical events in which Hagerman Valley IHNV types were introduced and became established in the lower Columbia River basin. However, the data also clearly indicates that the Hagerman Valley is not a continuous source of waterborne virus infecting salmonid stocks downstream.

Diseases of Aquatic Organisms↗
USGS2007

Occurrence and genetic typing of infectious hematopoietic necrosis virus in Kamchatka, Russia

Infectious hematopoietic necrosis virus (IHNV) is a well known rhabdoviral pathogen of salmonid fish in North America that has become established in Asia and Europe. On the Pacific coast of Russia, IHNV was first detected in hatchery sockeye from the Kamchatka Peninsula in 2001. Results of virological examinations of over 10 000 wild and cultured salmonid fish from Kamchatka during 1996 to 2005 revealed IHNV in several sockeye salmon Oncorhynchus nerka populations. The virus was isolated from spawning adults and from juveniles undergoing epidemics in both hatchery and wild sockeye populations from the Bolshaya watershed. No virus was detected in 2 other water-sheds, or in species other than sockeye salmon. Genetic typing of 8 virus isolates by seguence analysis of partial glycoprotein and nucleocapsid genes revealed that they were genetically homogeneous and fell within the U genogroup of IHNV. In phylogenetic analyses, the Russian IHNV sequences were indistinguishable from the sequences of North American U genogroup isolates that occur throughout Alaska, British Columbia, Washington, and Oregon. The high similarity, and in some cases identity, between Russian and North American IHNV isolates suggests virus transmission or exposure to a common viral reservoir in the North Pacific Ocean. Inter-Research 2007.

Kamchatka↗
USGS2021

Integrating environmental DNA results with diverse data sets to improve biosurveillance of river health

Autonomous, robotic environmental (e)DNA samplers now make it possible for biological observations to match the scale and quality of abiotic measurements collected by automated sensor networks. Merging these automated data streams may allow for improved insight into biotic responses to environmental change and stressors. Here, we merged eDNA data collected by robotic samplers installed at three U.S. Geological Survey (USGS) streamgages with gridded daily weather data, and daily water quality and quantity data into a cloud-hosted database. The eDNA targets were a rare fish parasite and a more common salmonid fish. We then used computationally expedient Bayesian hierarchical occupancy models to evaluate associations between abiotic conditions and eDNA detections and to simulate how uncertainty in result interpretation changes with the frequency of autonomous robotic eDNA sample collection. We developed scripts to automate data merging, cleaning and analysis steps into a chained-step, workflow. We found that inclusion of abiotic covariates only provided improved insight for the more common salmonid fish since its DNA was more frequently detected. Rare fish parasite DNA was infrequently detected, which caused occupancy parameter estimates and covariate associations to have high uncertainty. Our simulations found that collecting samples at least once per day resulted in more detections and less parameter uncertainty than less frequent sampling. Our occupancy and simulation results together demonstrate the advantages of robotic eDNA samplers and how these samples can be combined with easy to acquire, publicly available data to foster real-time biosurveillance and forecasting.

Idaho, Wyoming, Montana↗
USGS2022

Comparative susceptibilities of selected California Chinook salmon and steelhead populations to isolates of L Genogroup Infectious Hematopoietic Necrosis Virus (IHNV)

Salmonid species demonstrate varied susceptibility to the viral pathogen infectious hematopoietic necrosis virus (IHNV). In California conservation hatcheries, juvenile Chinook salmon ( Oncorhynchus tshawytscha) have experienced disease outbreaks due to L genogroup IHNV since the 1940s, while indigenous steelhead (anadromous O. mykiss) appear relatively resistant. To characterize factors contributing to the losses of California salmonid fish due to IHNV, three populations of Chinook salmon and two populations of steelhead native to California watersheds were compared in controlled waterborne challenges with California L genogroup IHNV isolates at viral doses of 10 4 –10 6 pfu mL −1 . Chinook salmon fry were moderately to highly susceptible (CPM = 47–87%) when exposed to subgroup LI and LII IHNV. Susceptibility to mortality decreased with increasing age and also with a higher temperature. Mortality for steelhead fry exposed to two IHNV isolates was low (CPM = 1.3–33%). There was little intraspecies variation in susceptibility among populations of Chinook salmon and no differences in virulence between viruses strains. Viral persistence was demonstrated by the isolation of low levels of infectious IHNV from the skin of two juvenile Chinook salmon at 215 d post exposure. The persistence of the virus among Chinook salmon used for stocking into Lake Oroville may be an explanation for the severe epidemics of IHN at the Feather River hatchery in 1998–2002.

California↗
USGS2021

Evidence for the use of mucus swabs to detect Renibacterium salmoninarum in brook trout

Efforts to advance fish health diagnostics have been highlighted in many studies to improve the detection of pathogens in aquaculture facilities and wild fish populations. Typically, the detection of a pathogen has required sacrificing fish; however, many hatcheries have valuable and sometimes irreplaceable broodstocks, and lethal sampling is undesirable. Therefore, the development of non-lethal detection methods is a high priority. The goal of our study was to compare non-lethal sampling methods with standardized lethal kidney tissue sampling that is used to detect Renibacterium salmoninarum infections in salmonids. We collected anal, buccal, and mucus swabs (non-lethal qPCR) and kidney tissue samples (lethal DFAT) from 72 adult brook trout ( Salvelinus fontinalis ) reared at the Colorado Parks and Wildlife Pitkin Brood Unit and tested each sample to assess R. salmoninarum infections. Standard kidney tissue detected R. salmoninarum 1.59 times more often than mucus swabs, compared to 10.43 and 13.16 times more often than buccal or anal swabs, respectively, indicating mucus swabs were the most effective and may be a useful non-lethal method. Our study highlights the potential of non-lethal mucus swabs to sample for R. salmoninarum and suggests future studies are needed to refine this technique for use in aquaculture facilities and wild populations of inland salmonids.

Pathogens↗
USGS2022

Hydroclimatic conditions, wildfire, and species assemblages influence co-occurrence of bull trout and tailed frogs in northern Rocky Mountain streams

Although bull trout ( Salvelinus confluentus ) and tailed frogs ( Ascaphus montanus ) have co-existed in forested Pacific Northwest streams for millennia, these iconic cold-water specialists are experiencing rapid environmental change caused by a warming climate and enhanced wildfire activity. Our goal was to inform future conservation by examining the habitat associations of each species and conditions that facilitate co-occupancy. We repurposed data from previous studies in the northern Rocky Mountains to assess the efficacy of bull trout electrofishing surveys for determining the occurrence of tailed frogs and the predictive capacity of habitat covariates derived from in-stream measurements and geospatial sources to model distributions of both species. Electrofishing reliably detected frog presence (89.2% rate). Both species were strongly associated with stream temperature and flow regime characteristics, and less responsive to riparian canopy cover, slope, and other salmonids. Tailed frogs were also sensitive to wildfire, with occupancy probability peaking around 80 years after a fire. Co-occupancy was most probable in locations with low-to-moderate frequencies of high winter flow events, few other salmonids, a low base-flow index, and intermediate years since fire. The distributions of these species appear to be sensitive to environmental conditions that are changing this century in forests of the northern Rocky Mountains. The amplification of climate-driven effects after wildfire may prove to be particularly problematic in the future. Habitat differences between these two species, considered to be headwater specialists, suggest that conservation measures designed for one may not fully protect the other. Additional studies involving future climate and wildfire scenarios are needed to assess broader conservation strategies and the potential to identify refuge streams where both species are likely to persist, or complementary streams where each could exist separately into the future.

Idaho, Montana↗
USGS2018

Exposure-related effects of Zequanox on juvenile lake sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush)

The environmental fate, persistence, and non-target animal impacts of traditional molluscicides for zebra, Dreissena polymorpha , and quagga, D. bugensis , mussel control led to the development of the biomolluscicide Zequanox. Although previous research has demonstrated the specificity of Zequanox, one study indicated sensitivity of salmonids and lake sturgeon, Acipenser fulvescens , following non-label compliant exposures to Zequanox. This study was conducted to evaluate sublethal and lethal impacts of Zequanox exposure on juvenile lake sturgeon and lake trout, Salvelinus namaycush , following applications that were conducted in a manner consistent with the Zequanox product label. Fish were exposed to 50 or 100 mg/L of Zequanox as active ingredient for 8 h and then held for 33 d to evaluate latent impacts. No acute mortality was observed in either species; however, significant latent mortality (P < 0.01, df = 9; 46.2%) was observed in lake trout that were exposed to the highest dose of Zequanox. Statistically significant (P < 0.03, df = 9), but biologically minimal differences were observed in the weight (range 20.17 to 21.49 g) of surviving lake sturgeon at the termination of the 33 d post-exposure observation period. Statistically significant (P < 0.05, df = 9) and biologically considerable differences were observed in the weight (range 6.19 to 9.55 g) of surviving lake trout at the termination of the 33 d post-exposure observation period. Histologic evaluation of lake trout gastrointestinal tracts suggests that the mode of action in lake trout is different from the mode of action that induces zebra and quagga mussel mortality. Further research could determine the sensitivity of other salmonid species to Zequanox and determine if native fish will avoid Zequanox treated water.

Management of Biological Invasions↗
USGS2002

Abundances of northwestern salamander larvae in montane lakes with and without fish, Mount Rainier National Park, Washington

In Mount Rainier National Park, the northwestern salamander usually inhabits relatively large and deep lakes and ponds (average size = 0.3 ha; average depth > 2 m) that contain flocculent, organic bottom sediments and abundant coarse wood. Prior to 1970, salmonids were introduced into many of the park's lakes and ponds that were typical habitat of the northwestern salamander. The objective of this study was to compare, in lakes and ponds with suitable habitat characteristics for northwestern salamanders, the observed abundances of larvae in takes and ponds with and without these introduced salmonids. Day surveys of 61 lakes were conducted between 1993 and 1999. Fish were limited to takes and ponds deeper than 2 in. For the 48 lakes and ponds deeper than 2 in (i.e., 25 fishless lakes and 23 fish lakes), the mean and median observed abundances of northwestern salamander larvae in fishless lakes and ponds was significantly greater than the mean and median observed abundances of larvae in lakes and ponds with fish. Northwestern salamander larvae were not observed in 11 fish lakes. These lakes were similar in median elevation, surface area, and maximum depth to the fishless lakes. The 12 fish lakes with observed larvae were significantly lower in median elevation, larger in median surface area, and deeper in median maximum depth than the fishless lakes. Low to null observed abundances of northwestern salamander larvae in lakes and ponds with fish were attributed to a combination of fish predation of larvae and changes in larval behavior.

Northwest Science↗
USGS2005

A synthesis of ecological and fish-community changes in Lake Ontario, 1970-2000

We assessed stressors associated with ecological and fishcommunity changes in Lake Ontario since 1970, when the first symposium on Salmonid Communities in Oligotrophic Lakes (SCOL I) was held (J. Fish. Res. Board Can. 29: 613-616). Phosphorus controls implemented in the early 1970s were undeniably successful; lower food-web studies showed declines in algal abundance and epilimnetic zooplankton production and a shift in pelagic primary productivity toward smaller organisms. Stressors on the fish community prior to 1970 such as exploitation, sea lamprey ( Petromyzon marinus ) predation, and effects of nuisance populations of alewife ( Alosa pseudoharengus ) were largely ameliorated by the 1990s. The alewife became a pivotal species supporting a multi-million-dollar salmonid sport fishery, but alewife-induced thiamine deficiency continued to hamper restoration and sustainability of native lake trout ( Salvelinus namaycush ). Expanding salmonine populations dependent on alewife raised concerns about predator demand and prey supply, leading to reductions in salmonine stocking in the early 1990s. Relaxation of the predation impact by alewives and their shift to deeper water allowed recovery of native fishes such as threespine stickleback (Gasterosteus aculeatus) and emerald shiner ( Notropis atherinoides ). The return of the Lake Ontario ecosystem to historical conditions has been impeded by unplanned introductions. Establishment of Dreissena spp. led to increased water clarity and increased vectoring of lower trophic-level production to benthic habitats and contributed to the collapse of Diporeia spp. populations, behavioral modifications of key fish species, and the decline of native lake whitefish (Coregonus clupeaformis ). Despite reduced productivity, exotic-species introductions, and changes in the fish community, offshore Mysis relicta populations remained relatively stable. The effects of climate and climate change on the population abundance and dynamics of Lake Ontario fish were unknown at the time of SCOL I, but a temperature-time series begun in the late 1950s in the Kingston Basin has since provided evidence of climate warming and associated fish-community changes. We should expect ecological surprises in the coming decades that will challenge scientists and fishery managers especially as they face new exotic species, climate warming, and escalating stakeholder demands on the resource. Continuous long-term ecological studies were critical for interpreting changes in Lake Ontario's fish community over the past three decades and will be essential in the future for both scientific understanding and management of the fishery.

Technical Report↗
USGS2000

Mortality and kidney histopathology of chinook salmon Oncorhynchus tshawytscha exposed to virulent and attenuated Renibacterium salmoninarum strains

An isolate of Renibacterium salmoninarum (strain MT 239) exhibiting reduced virulence in rainbow trout Oncorhynchus mykiss was tested for its ability to cause bacterial kidney disease (BKD) in chinook salmon Oncorhynchus tshawytscha, a salmonid species more susceptible to BKD. Juvenile chinook salmon were exposed to either 33209, the American Type Culture Collection type strain of R. salmoninarum, or to MT 239, by an intraperitoneal injection of 1 ?? 103 or 1 ?? 106 bacteria fish-1, or by a 24 h immersion in 1 ?? 105 or 1 ?? 107 bacteria ml-1. For 22 wk fish were held in 12??C water and monitored for mortality. Fish were sampled periodically for histological examination of kidney tissues. In contrast to fish exposed to the high dose of strain 33209 by either injection or immersion, none of the fish exposed to strain MT 239 by either route exhibited gross clinical signs or histopathological changes indicative of BKD. However, the MT 239 strain was detected by the direct fluorescent antibody technique in 4 fish that died up to 11 wk after the injection challenge and in 5 fish that died up to 20 wk after the immersion challenge. Viable MT 239 was isolated in culture from 3 fish that died up to 13 wk after the immersion challenge. Total mortality in groups injected with the high dose of strain MT 239 (12%) was also significantly lower (p < 0.05) than mortality in groups injected with strain 33209 (73%). These data indicate that the attenuated virulence observed with MT 239 in rainbow trout also occurs in a salmonid species highly susceptible to BKD. The reasons for the attenuated virulence of MT 239 were not determined but may be related to the reduced levels of the putative virulence protein p57 associated with this strain.

Diseases of Aquatic Organisms↗
USGS2008

Thiamine Deficiency Complex Workshop final report: November 6-7, 2008, Ann Arbor, MI

Fry mortality which was first observed in the late 1960s in Great Lakes salmonines and in Baltic Sea salmon in 1974 has now been linked to thiamine deficiency (historically referred to as Early Mortality Syndrome, or EMS and M74, respectively). Over the past 14 years significant strides have been made in our understanding of this perplexing problem. It is now known that thiamine deficiency causes embryonic mortality in these salmonids. Both overt mortality and secondary effects of thiamine deficiency are observed in juvenile and adult animals. Collectively the morbidity and mortality (fry and adult mortality, secondary metabolic and behavior affects in juveniles and adult fish) are referred to as Thiamine Deficiency Complex (TDC). A workshop was held in Ann Arbor, MI on 6-7 November 2008 that brought together 38 federal, state, provincial, tribal and university scientists to share information, present data and discuss the latest observations on thiamine status of aquatic animals with thiamine deficiency and the causative agent, thiaminase. Twenty presentations (13 oral and 7 posters) detailed current knowledge. In Lake Huron, low alewife Alosa pseudoharengus abundance has persisted and egg thiamine concentrations in salmonines continue to increase, along with evidence of natural reproduction in lake trout Salvelinus namaycush. Lake Michigan Chinook salmon Oncorhynchus tshawytscha appear to have a lower thiamine requirement than other salmonids in the lake. Lake Ontario American eel Anguilla rostrata foraging on alewife have approximately one third the muscle thiamine compared to eels not feeding on alewife, suggesting that eels may be suffering from thiamine deficiency. Secondary effects of low thiamine exist in Great Lakes salmonines and should not be ignored. Thiaminase activity in dreissenid mussels is extremely high but a connection to TDC has not been made. Thiaminase in net plankton was found more consistently in lakes Michigan and Ontario than other lakes evaluated. The biological role of thiaminase I, associated with thiamine deficiency, remains to be determined whereas thiaminase II has been reported to be part of a salvage pathway leading to thiamine synthesis. The use of gene array technology and 3-dimensional histology is adding new understanding to the affects of thiamine deficiency. Research is needed to determine the thiamine status of species feeding on dreissenids, the environmental sources of thiaminase and the biological role of thiaminase I.

Research Status Report↗