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At least 613 records · Page 34Linked to original sources
USGS1995

Radon-222 concentrations in ground water and soil gas on Indian reservations in Wisconsin

The weighted average radon-222 concentration of indoor air in homes located on Wisconsin Indian Reservations is 5.8 picocuries per liter, which exceeds the U.S. Environmental Protection Agency action limit of 4 picocuries per liter. Ground water is the principle source of drinking water on Wisconsin Indian Reservations and generally accounts for about 5 percent of the total indoor air radon-222 concentrations found in homes. To determine the distribution of radon-222, ground water from 29 private and community Wisconsin Indian Reservation wells and soil gas at a depth of about 3 feet below land surface adjacent to the wells were sampled. Sites with wells were distributed among the 11 Wisconsin Indian Reservations so that each Reservation contained at least 2 sites. The remaining seven sites were divided among the Reservation by acreage held by each tribe. Ground-water samples were collected using a syringe technique after the wells had been purged sufficiently to reach chemical stability. Samples were then sent by overnight mail to the U.S. Geological Survey, National Water Quality Lab for analysis by liquid scintillation counting. Soil gas was collected in Lucas cells, using a small-diameter soil probe and peristaltic pump. The cells were then analyzed by Lucas cell alpha scintillation counting with a portable radon detector. The highest radon-222 concentrations in ground water and soil gas are from sites with wells finished in the crystalline bedrock aquifer. Radon-222 concentrations in 29 ground-water samples collected from the sand and gravel and sedimentary and crystalline bedrock aquifers range from 260 to 22,000 picocuries per liter with a median concentration of 560 picocuries per liter. Only 2 of the 29 ground-water samples have radon-222 concentrations less than the U.S. Environmental Protection Agency proposed standard of 300 picocuries per liter. The highest radon-222 concentrations were found in ground water from wells in Shawano, Menominee, Forest, and Marathon counties. Radon-222 concentrations in soil gas range from 130 to 7,810 picocuries per liter with a median concentration of 560 picocuries per liter. For sites with wells finished in the sand and gravel aquifer, the coefficient of determination (R2) of the regression of concentration of radon-222 in ground water as a function of well depth is 0.003 and the significance level is 0.32, which indicates that there is not a statistically significant relation between radon-222 concentrations in ground water and well depth. The coefficient of determination of the regression of radon-222 in ground water and soil gas is 0.19 and the root mean square error of the regression line is 271 picocuries per liter. Even though the significance level (0.036) indicates a statistical relation, the root mean square error of the regression is so large that the regression equation would not give reliable predictions. Because of an inadequate number of samples, similar statistical analyses could not be performed for sites with wells finished in the crystalline and sedimentary bedrock aquifers.

Wisconsin↗
USGS1996

Description and field analysis of a coupled ground-water/surface-water flow model (MODFLOW/BRANCH) with modifications for structures and wetlands in southern Dade County, Florida

A coupled surface-water model (BRANCH) and ground-water model (MODFLOW) model were tested to simulate the interacting wetlands/surface-water/ ground-water system of southern Dade County. Several options created for the MODFLOW ground- ground-water model were used in representing this field situation. The primary option is the MODBRANCH interfacing software, which allows leakage to be accounted for between the MODFLOW ground-water model and the BRANCH dynamic model for simulation of flow in an interconnected network of open channels. A modification to an existing software routine, which is referred to as BCF2, allows cells in MODFLOW to rewet when dry--a requirement in representing the seasonal wetlands in Dade County. A companion to BCF2 is the modified evapotranspiration routine EVT2. The EVT2 routine changes the cells where evapotranspiration occurs, depending on which cells are wet. The Streamlink package represents direct connections between the canals and wetlands at locations where canals open directly into overland flow. Within the BRANCH model, the capability to represent the numerous hydraulic structures, gated spillways, gated culverts, and pumps was added. The application of these modifications to model surface-water/ground-water interactions in southern Dade County demonstrated the usefulness of the coupled MODFLOW/BRANCH model. Ground-water and surface-water flows are both simulated with dynamic models. Flow exchange between models, intermittent wetting and drying, evapotranspiration, and hydraulic structure operations are all represented appropriately. Comparison was made with a simulation using the RIV1 package instead of MODBRANCH to represent the canals. RIV1 represents the canals by user-defined stages, and computes leakage to the aquifer. Greater accuracy in reproducing measured ground- water heads was achieved with MODBRANCH, which also computes dynamic flow conditions in the canals, unlike RIV1. The surface-water integrated flow and transport two-dimensional model (SWIFT2D) was also applied to the southeastern coastal wetlands for comparison with the wetlands flow approximation made in MODFLOW. MODFLOW simulates the wetlands as a highly conductive upper layer of the aquifer, whereas SWIFT2D solves the hydrodynamic equations. Comparison in this limited test demonstrated no specific advantage for either method of representation. However, much additional testing on a wider variety of geometric and hydraulic situations, such as in areas with greater tidal or other dynamic forcing effects, is needed to make definite conclusions. A submodel of the existing southern Dade County model schematization was used to examine water-delivery alternatives proposed by the U.S. Army Corps of Engineers. For this application, the coupled MODFLOW/BRANCH model was used as a design tool. A new canal and several pumps to be tested to maintain lower water levels in a residential area (while water levels in the Everglades are raised) were added to the model schematization. The pumps were assumed to have infinite supply capacity in the model so that their maximum pumping rates during the simulation could be used to determine pump sizes.

Water-Resources Investigations Report↗
USGS1993

Numerical simulation of ground-water flow in the central part of the western San Joaquin Valley, California

The occurrence of selenium in agricultural drain water in the central part of the western San Joaquin Valley, California, has focused concern on strategies for managing shallow, saline ground water. To assess alternatives to agricultural drains, a three-dimensional, finite-difference numerical model of the regional groundwater flow system was developed. This report documents the mathematical approach used to model the flow system, the data base on which the model is based, and the methods used to calibrate the model. The 550-square-mile study area includes parts of the Panoche Creek alluvial fan and parts of the Little Panoche Creek and Cantua Creek alluvial fans. The model simulates transient flow in the semiconfined and confined zones above and below the Corcoran Clay Member of the Tulare Formation of Pleistocene age. The model incorporates areally distributed ground-water recharge, areally and vertically distributed pumping, regional-collector drains in the Wesdands Water District (operative from 1980 to 1985), on-farm drains in parts of the Panoche, Broadview, and Firebaugh Water Districts, and bare-soil evaporation (which occurs if the water table is within 7 feet of land surface). The model also incorporates texture-based estimates of hydraulic conductivity, where texture is defined as the fraction of coarse-grained deposits present in a given subsurface interval. The numerical model was developed using hydrologic data from 1972 to 1988. Most of the parameters incorporated into the model were evaluated independently of the model, including system geometry, the distribution of texture, the altitudes of the water table and potentiometric surface of the confined zone in 1972 (initial condition), the hydraulic conductivity of coarse-grained deposits derived from the Coast Ranges, the hydraulic conductivity of coarse-grained deposits derived from the Sierra Nevada, specific storage, recharge, pumping, and parameters needed to incorporate drains and bare-soil evaporation. Four parameters were calibration variables: the hydraulic conductivity of fine-grained deposits in the semiconfined zone, the hydraulic conductivity of the Corcoran Clay Member, specific yield, and the transmissivity of the confined zone. The model was calibrated in two phases. In the first phase, a steady-state model of the ground-water flow system in 1984 was used to constrain the relation between the hydraulic conductivity of fine-grained deposits in the semiconfined zone and the hydraulic conductivity of the Corcoran Clay Member, thus reducing the number of independent variables from four to three. In the second phase of calibration, the change in altitude of the water table from 1972 to 1984, the change in altitude of the potentiometric surface of the confined zone from 1972 to 1984, and the number of model cells subject to bare-soil evaporation from 1972 to 1988 were used to evaluate the remaining three variables. The calibrated model reproduces the average change in water-table altitude (1972-84) to within 0.4 foot (average measured change 11.5 feet) and the average change in confined zone head (1972- 84) to within 19 feet (average measured change 120 feet). The simulated time-series record of the total number of model cells subject to bare-soil evaporation (each cell is 1 mile square) is within the range of the measured data. The measured values are at a minimum in October and a maximum in July. The October values ranged from 103 in 1972 to 132 in 1984 (the drains were closed in 1985) to 151 in 1988. The July values ranged from 144 in 1973 to 198 in 1984, to 204 in 1988. The simulated values ranged from 103 in 1972 to 161 in 1984, to 208 in 1988.

Water Supply Paper↗
USGS1997

Simulation of the water-table altitude in the Biscayne Aquifer, southern Dade County, Florida, water years 1945-89

A digital model of the flow system in the highly permeable surficial Biscayne aquifer of southern Dade County, Florida, was constructed for the purposes of better understanding processes that influence the flow system and of supporting the construction of a subregional model of the transport of brackish water from a flowing artesian well. Problems that needed resolution in this endeavor included the development of methods to represent the influence of flowing surface water in seasonally inundated wetlands and the influence of a network of controlled canals developed in stages during the simulation time period (water years 1945-89). An additional problem was the general lack of natural aquifer boundaries near the boundaries of the study area. The model construction was based on a conceptual description of the Biscayne aquifer developed from the results of previous U.S. Geological Survey investigations. Modifications were made to an existing three-dimensional finite-difference simulator of ground-water flow to enable an upper layer of the grid to represent seasonally occurring overland sheet flow in a series of transient simulations of water levels from 1945 to 1989. A rewetting procedure was developed for the simulator that permitted re-saturation of cells in this layer when the wet season recurred. An �equivalent hydraulic conductivity� coefficient was assigned to the overland flow layer that was analogous, subject to various approximations, to the use of the Manning equation. The surficial semi-confining peat and marl layers, levees, canals, and control structures were also represented as part of the model grid with the appropriate choices of hydraulic coefficient values. For most of the Biscayne aquifer grid cells, the value assigned to hydraulic conductivity for model calibration was 30,000 feet per day and the value assigned to porosity was 20 percent. Boundary conditions were specified near data sites having long-term records of surface-water stages or water-table altitudes, and modifications to the simulator permitted the specification of time-varying pressures at boundary grid cells. Rainfall data from a station in Homestead generally were used as an a really uniform rainfall specification throughout the modeled region. Maximum evapotranspiration rates ranged seasonally from a minimum of 0.08 inch per day in January to a maximum of 0.21 inch per day between June and October. Shallow-root and deep-root zone depths for the evapotranspiration calculation were 3 and 20 feet in the coastal ridge and were 0.10 and 5 feet in the glades regions where peat and marl covers occurred. Results of sensitivity analyses indicated that the simulations of stages and water levels were relatively unresponsive to 50 percent changes in aquifer hydraulic conductivity, porosity, and the equivalent hydraulic conductivity of overland flow. However, 20 percent changes in rainfall and maximum evapotranspiration rates produced significantly different water levels, as did interchange of coastal ridge and glades deep-root zone (extinction) depths. Water levels were simulated very well at most measurement sites. Sensitivity analyses illustrated the significant influence of the uncontrolled agricultural drainage canals on pre-1968 regional water levels and the further influence of Black Creek Canal in draining a region of high water after 1961. Other analyses indicated that the flood-control system of 1968-82 lowered peak water levels in the affected region by as much as 1.5 feet in the wet summers of 1968, 1969, and 1981, and that Levee 67 Extended channeled flows from the S-12 spillway structures and raised overland flow stages in Shark River Slough. Hypothetical scenarios of well-field pumping in the vicinity of Levee 31N indicated that the pumping induced a significant amount of recharge from the adjacent borrow canal, the degree of which depended on the distance between the canal and the well field. The computed ratio of evapotranspiration to rainfall recharge ranged from 88 to 94 percent during water years 1945-82. The ratio increased to about 97.9 percent during water years 1983-89, possibly because of changing water-management practices and deficient rainfall.

Florida↗
USGS2007

Effects of nutrient enrichment on Prymnesium parvum population dynamics and toxicity: Results from field experiments, Lake Possum Kingdom, USA

Large fish kills associated with toxic populations of the haptophyte Prymnesium parvum occur worldwide. In the past 5 yr, incidences of P. parvum blooms in inland water bodies of Texas (USA) have increased dramatically, where cell densities in excess of 1 × 10 7 cells l –1 are typically observed. We conducted field experiments (Lake Possum Kingdom) during the fall and early spring of 28 d duration using 24 enclosures of 1.57 m 3 each. The experiments investigated the effect of nutrient enrichment, immigration of P. parvum and addition of barley straw extract on phytoplankton biomass and assemblage structure, P. parvum population density, zooplankton biomass and assemblage structure, bacteria, and toxicity. Nutrient enrichment stimulated P. parvum population growth beyond bloom proportions (>1 × 10 7 cells l –1 ). However, P. parvum did not dominate the assemblage under these conditions, as it does during natural blooms. Instead, euglenophytes and chlorophytes dominated. Toxicity, estimated using fish ( Pimephales promelas ) and cladoceran ( Daphnia magna ) bioassays and which is linked to P. parvum ’s allelopathic and mixotrophic effectiveness, was greatly reduced (eliminated in many cases) under conditions of nutrient enrichment. The suppression of toxicity by nutrient addition suggested that targeted and time-limited nutrient manipulations might be used to mitigate the effects of P. parvum blooms. Immigration of P. parvum into natural assemblages and addition of barley straw extract had no significant effect on plankton dynamics. Inter-Research 2007.

Texas↗
USGS1989

Gill tissue reactions in walleye Stizostedion vitreum vitreum and common carp Cyprinus carpio to glochidia of the freshwater mussel Lampsilis radiata siliquoidea

The glochidia of many freshwater mussels, which are obligate parasites on the gills, fins, and other body parts of specific fishes, attach to a suitable host, become encapsulated, and develop to the free-living juvenile stage. Using light and electron microscopy we compared gill tissue reactions in a suitable host (walleye Stizostedion vitreum vitreum ) and unsuitable host (common carp Cyprinus carpio ) infected with Lampsilis radiata siliquoidea . Encapsulation of glochidia on walleye gills was completed by 6 h post-infection at 20 to 22°C. Capsular formation and compaction were accompanied by a general increase in epithelioid cells. Fibrotic material appeared in capsules at about 48 h and virtually filled capsular cells from about Day 5 to Day 11 post-infection. Liberation of juvenile mussels was accompanied by thinning of the capsule from about Day 11 to Day l7. Although glochidia attached to the gills of common carp, few became encapsulated. By 48 h post-infection, preliminary capsular growth was evident and necrotic cells and cellular debris appeared at the edges of the growth. However, all glochidia were sloughed from carp gills by 60 h. Host specificity of L. radiata siliquoidea apparently depended on a combination of the attachment response of glochidia, differences in the encapsulation process, and tissue reactions in the fish.

Diseases of Aquatic Organisms↗
USGS1995

Characterization of a rhabdovirus isolated from carpione Salmo trutta carpio in Italy

A virus, strain 583, was isolated from carpione Salmo trutta carpio fry exhibiting high mortality. The virus was not neutralized by rabbit antisera against the fish rhabdoviruses viral haemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus, eel rhabdovirus European X, spring viraemia of carp virus or pike fry rhabdovirus, or against the birnavirus infectious pancreatic necrosis virus. The virus replicated in several fish cell lines incubated at 20 to 25*C and grew optimally in the bluegill fry (BF-2) and fathead minnow (FHM) cell lines. Electron microscopy of infected BF-2 cell cultures revealed the presence of typical rhabdovirus particles, and immunofluorescent staining was observed using various polyclonal and monoclonal antibodies (MAbs) against Egtved virus, the causative agent of viral haemorrhagic septicaemia. The staining by a MAb against the nucleoprotein (N) of VHSV was particularly strong, a MAb against the glycoprotein (G) gave a moderate reaction, whereas a second MAb against the G protein and MAbs against the matrix proteins, M_(1) and M_(2), of VHSV did not react. Fluorescence titres using 3 rabbit antisera against whole Egtved virus varied between negative and moderately positive. Western blotting using polyclonal and monoclonal sera confirmed that both the N and G proteins of the carpione virus shared some epitopes with those of VHSV, but the M_(1) and M_(2) proteins did not. SDS-PAGE showed the structural proteins of the carpione virus produced a pattern typical of members of the Lyssavirus genus of the Rhabdoviridae and the molecular weights were very similar to those of VHSV, except for the M_(2) protein which was somewhat smaller. Infection trials showed the carpione virus induced high mortalities in carpione fry but not in rainbow trout Oncorhynchus mykiss fry. The carpione virus was clearly distinguishable from Egtved virus despite limited serological cross reaction. Since it was also easily distinguishable by immunofluorescence from the other fish rhabdoviruses included in the present study as well as in studies published elsewhere, it is concluded that the virus is a previously undescribed one. It is proposed that the virus be given the preliminary designation 'carpione rhabdovirus'.

Diseases of Aquatic Organisms↗
USGS2019

Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola

The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cyprini cell line was refractory at all temperatures evaluated. High throughput sequencing technologies facilitated the complete genome sequencing of this virus utilizing ribosomal RNA-depleted RNA extracted from infected CHSE-214 cells. Conventional PCR primer sets were developed for the detection and confirmation of this virus to assist diagnostic screening methods. Phylogenetic analysis suggests this virus belongs to the Aquareovirus A genus. This research provides requisite initial data critical to support hatchery and refugia biosecurity measures for this endangered species.

Diseases of Aquatic Organisms↗
USGS2012

Variations of transcript profiles between sea otters Enhydra lutris from Prince William Sound, Alaska, and clinically normal reference otters

Development of blood leukocyte gene transcript profiles has the potential to expand condition assessments beyond those currently available to evaluate wildlife health, including sea otters Enhydra lutris , both individually and as populations. The 10 genes targeted in our study represent multiple physiological systems that play a role in immuno-modulation, inflammation, cell protection, tumor suppression, cellular stress-response, xenobiotic metabolizing enzymes, and antioxidant enzymes. These genes can be modified by biological, physical, or anthropogenic impacts and consequently provide information on the general type of stressors present in a given environment. We compared gene transcript profiles of sea otters sampled in 2008 among areas within Prince William Sound impacted to varying degrees by the 1989 ‘Exxon Valdez’ oil spill with those of captive and wild reference sea otters. Profiles of sea otters from Prince William Sound showed elevated transcription in genes associated with tumor formation, cell death, organic exposure, inflammation, and viral exposure when compared to the reference sea otter group, indicating possible recent and chronic exposure to organic contaminants. Sea otters from historically designated oiled areas within Prince William Sound 19 yr after the oil spill had higher transcription of genes associated with tumor formation, cell death, heat shock, and inflammation than those from areas designated as less impacted by the spill.

Alaska↗
USGS2014

Microbial aggregates within tissues infect a diversity of corals throughout the Indo-Pacific

Coral reefs are highly diverse ecosystems where symbioses play a pivotal role. Corals contain cell-associated microbial aggregates (CAMA), yet little is known about how widespread they are among coral species or the nature of the symbiotic relationship. Using histology, we found CAMA within 24 species of corals from 6 genera from Hawaii, American Samoa, Palmyra, Johnston Atoll, Guam, and Australia. Prevalence (%) of infection varied among coral genera: Acropora, Porites, and Pocillopora were commonly infected whereas Montipora were not. Acropora from the Western Pacific were significantly more likely to be infected with CAMA than those from the Central Pacific, whereas the reverse was true for Porites. Compared with apparently healthy colonies, tissues from diseased colonies were significantly more likely to have both surface and basal body walls infected. The close association of CAMA with host cells in numerous species of apparently healthy corals and lack of associated cell pathology reveals an intimate agent-host association. Furthermore, CAMA are Gram negative and in some corals may be related to chlamydia or rickettsia. We propose that CAMA in adult corals are facultative secondary symbionts that could play an important ecological role in some dominant coral genera in the Indo-Pacific. CAMA are important in the life histories of other animals, and more work is needed to understand their role in the distribution, evolution, physiology, and immunology of reef corals.

Marine Ecology Progress Series↗
USGS2022

Poplar Island: Understanding the development of a beneficial use restoration site

Poplar Island, like many other islands throughout the Chesapeake Bay, eroded from 460 hectares in 1847 to only 1.5 hectares by the 1990’s. However, the U.S. Army Corps of Engineers, Maryland Department of Transportation, and numerous other state and federal agencies selected this site as the location of a beneficial use project aimed at restoring remote island habitat in the Chesapeake Bay using clean dredge material. While monitoring efforts since the beginning of restoration efforts have documented extensive use of Poplar Island by numerous species across multiple taxa, these efforts have previously lacked context regarding underlying habitat patterns. However, such information is especially important on a site like Poplar Island where habitat distribution and availability can change dramatically between years due to ongoing construction efforts. To address this information gap we digitized annual aerial imagery of Poplar Island from 2006-2017 into 20 habitat classes. The resulting data layers demonstrate the transition of cells along the eastern side of the island from undeveloped cells to planted marsh cells, which matches trends seen in avian monitoring data. Similarly, our data display changes in the distribution of specific resources such as sand across the island, and how individual locations of interest such as constructed habitat islands have evolved over time. We believe that these data will provide critical insight into the factors influencing wildlife distribution patterns on Poplar Island, and will allow for the identification of management actions that may either be targeted or avoided in the planning of future beneficial use projects.

Maryland↗
USGS2012

Immunity to fish rhabdoviruses

Members of the family Rhabdoviridae are single-stranded RNA viruses and globally important pathogens of wild and cultured fish and thus relatively well studied in their respective hosts or other model systems. Here, we review the protective immune mechanisms that fish mount in response to rhabdovirus infections. Teleost fish possess the principal components of innate and adaptive immunity found in other vertebrates. Neutralizing antibodies are critical for long-term protection from fish rhabdoviruses, but several studies also indicate a role for cell-mediated immunity. Survival of acute rhabdoviral infection is also dependent on innate immunity, particularly the interferon (IFN) system that is rapidly induced in response to infection. Paradoxically, rhabdoviruses are sensitive to the effects of IFN but virulent rhabdoviruses can continue to replicate owing to the abilities of the matrix (M) protein to mediate host-cell shutoff and the non-virion (NV) protein to subvert programmed cell death and suppress functional IFN. While many basic features of the fish immune response to rhabdovirus infections are becoming better understood, much less is known about how factors in the environment affect the ecology of rhabdovirus infections in natural populations of aquatic animals.

Viruses↗
USGS2015

Downscaling global land-use/land-cover projections for use in region-level state-and-transition simulation modeling

Global land-use/land-cover (LULC) change projections and historical datasets are typically available at coarse grid resolutions and are often incompatible with modeling applications at local to regional scales. The difficulty of downscaling and reapportioning global gridded LULC change projections to regional boundaries is a barrier to the use of these datasets in a state-and-transition simulation model (STSM) framework. Here we compare three downscaling techniques to transform gridded LULC transitions into spatial scales and thematic LULC classes appropriate for use in a regional STSM. For each downscaling approach, Intergovernmental Panel on Climate Change (IPCC) Representative Concentration Pathway (RCP) LULC projections, at the 0.5 × 0.5 cell resolution, were downscaled to seven Level III ecoregions in the Pacific Northwest, United States. RCP transition values at each cell were downscaled based on the proportional distribution between ecoregions of (1) cell area, (2) land-cover composition derived from remotely-sensed imagery, and (3) historic LULC transition values from a LULC history database. Resulting downscaled LULC transition values were aggregated according to their bounding ecoregion and “cross-walked” to relevant LULC classes. Ecoregion-level LULC transition values were applied in a STSM projecting LULC change between 2005 and 2100. While each downscaling methods had advantages and disadvantages, downscaling using the historical land-use history dataset consistently apportioned RCP LULC transitions in agreement with historical observations. Regardless of the downscaling method, some LULC projections remain improbable and require further investigation.

AIMS Environmental Science↗
USGS1986

Immunoblot assay: a rapid and sensitive method for identification of salmonid fish viruses

An immunoblot assay was used to identify the viruses of infectious pancreatic necrosis, infectious hematopoietic necrosis, and viral hemorrhagic septicemia. Viral antigen in infected cell culture supernatant was adsorbed onto nitrocellulose membrane or Whatman 541 filter paper and detected by enzyme-linked immunosorbent assay techniques. The immunoblot assay took less than 4 hr to perform and required no special instrumentation. Assays using cell culture supernatant fluids showed immunoblot sensitivity was 10 5 –10 6 PFU/ml. Assay sensitivity, determined using purified virus, is 0.85–4.0 ng of viral antigen. The immunoblot assay was used to detect and identify virus in cell culture fluids.

Journal of Wildlife Diseases↗
USGS1991

Weights and hematology of wild black bears during hibernation

We compared weights and hematological profiles of adult (>3-yr-old) female black bears ( Ursus americanus ) during hibernation (after 8 January). We handled 28 bears one to four times (total of 47) over 4 yr of varying mast and berry production. Mean weight of lactating bears was greater ( P < 0.0001) than that of non-lactating females. White blood cells ( P < 0.05) and mean corpuscular volume ( P = 0.005) also differed between lactating and non-lactating bears. Hemoglobin ( P = 0.006) and mean corpuscular hemoglobin concentration ( P = 0.02) varied among years; values were lowest during 1975, following decreased precipitation and the occurrence of a second year of mast and berry crop shortages in a three-year period. Significant ( P < 0.05) interaction between reproductive status (lactating versus non-lactating) and study year for hemoglobin, red blood cells, and packed cell volume, and increased mean corpuscular volume, suggested a greater nutritional challenge for lactating females compared to non-lactating females during the 1975 denning season. Our data suggest that hematological characteristics of denning bears may be more sensitive than weights as indicators of annual changes in nutritional status; however, other influential factors, in addition to mast and berry crop production, remain to be examined.

Journal of Wildlife Diseases↗
USGS1992

Energy metabolism and hematology of white-tailed deer fawns

Resting metabolic rates, weight gains and hematologic profiles of six newborn, captive white-tailed deer ( Odocoileus virginianus ) fawns (four females, two males) were determined during the first 3 mo of life. Estimated mean daily weight gain of fawns was 0.2 kg. The regression equation for metabolic rate was: Metabolic rate (kcal/kg 0.75 /day) = 56.1 + 1.3 (age in days), r = 0.65, P < 0.001). Regression equations were also used to relate age to red blood cell count (RBC), hemoglobin concentration (Hb), packed cell volume, white blood cell count, mean corpuscular volume, mean corpuscular hemoglobin concentration (MCHC), and mean corpuscular hemoglobin. The age relationships of Hb, MCHC, and smaller RBC's were indicative of an increasing and more efficient oxygen-carrying and exchange capacity to fulfill the increasing metabolic demands for oxygen associated with increasing body size.

Journal of Wildlife Diseases↗
USGS1997

Hematologic and serum biochemical reference intervals for Florida panthers

Ninety-four blood samples were collected from 48 (29 males and 19 females) free-ranging Florida panthers ( Felis concolor coryi ) captured in southern Florida (USA) from 1983 to 1994 for routine hematological and serum biochemical analysis. Florida panthers in the northern portion of their range had significantly higher red blood cell (mean ± SD = 7.923 × 10 6 ± 0.854 × 10 6 /μl), hemoglobin (12.53 ± 1.66 g/dl), and packed cell volume (36.97 ± 4.27%) values compared to those of panthers localized in more southern parts of Florida (7.148 × 10 6 ± 1.045 × 10 6 /μl, 11.60 ± 1.62 g/dl, and 34.82 ± 5.99%, respectively). Adults had significantly higher mean serum total protein (7.50 ± 0.59 g/dl) and packed cell volume (36.90 ± 4.97%) values than juveniles (6.88 ± 0.49 g/dl and 34.54 ± 5.30%). However, mean serum albumin concentrations were significantly higher in juveniles (3.80 ± 0.26 g/dl) when compared to adult values (3.58 ± 0.26 g/dl). Mean serum calcium concentrations were significantly higher in juveniles (10.33 ± 0.39 mg/dl) than in adults (9.66 ± 0.45 mg/dl). Additionally, mean serum iron concentrations were significantly higher in those panthers of intergrade genetic stock compared to values in those of authentic genetic stock (105.6 ± 72.1 μg/dl versus 59.3 ± 19.7 μg/dl, respectively).

Florida↗
USGS1972

Some physiologic blood values of wild diving ducks

Blood samples were obtained from 54 canvasbacks ( Aythya valisineria ), 30 lesser scaup ( A. affinis ), 3 ring-necks ( A. collaris ), and 3 buffleheads ( Bucephala albeola ), which were wintering on Chesapeake Bay. These blood samples were used for: red blood cell counts (cans. 2.56 × 10 6 /mm 3 ; scaup 2.45 × 10 6 /mm 3 ; ring-necks 2.50 × 10 6 /mm 3 ; bufflehead 2.64 × 10 6 /mm 3 ), packed cell volume (cans. 52.2%; scaup 57.1%; ring-neck 49.1%; buffleheads 54.3%), total protein (cans. 4.4 g/100 ml; scaup 4.4 g/100 ml; ring-neck 3.5 g/100 ml; bufflehead 3.8 g/100 ml), erythrocyte measurements, and electrophoretic analysis of plasma and serum. Variations between individuals of the same species and sex were slight but a possible difference between male and female erythrocyte numbers and packed cell volumes was evident in the canvasback and scaup samples.

Maryland↗