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Detection of viral, bacterial, and protozoan pathogens and microbial source tracking markers in paired large- and small-volume water samples

When sampling for waterborne microbes, researchers may need to diverge from recommended sample volumes due to logistical constraints, novel targets, or challenging matrices, with little guidance about the potential impact on results. In field studies, we measured bacteria, viruses, and protozoa (15 quantitative polymerase chain reaction assays) in paired large- and small-volume samples to evaluate method performance and relevant factors. Concordance between methods was low. Large-volume ultrafiltration yielded more detections than small-volume sampling, especially for pathogens in groundwater. Greater microbial concentrations were associated with more frequent detections in small-volume samples and greater concordance between paired samples. Large-volume samples appeared to be more susceptible to diminished sensitivity from complex sample matrices. In laboratory studies, recovery of microbes was poorer for large- than small-volume methods, although large-volume methods more reliably detected low-concentration targets. Large-volume samples were less stable than small-volume samples during storage. Overall, large-volume sampling was superior for detecting pathogens but may underestimate concentrations; small-volume sampling was more prone to false negatives but was adequate when concentrations were relatively high, like we observed for microbial source tracking in surface waters.

Wisconsin

Apatite geo-thermochronology and geochemistry constrain Oligocene-Miocene growth and geodynamics of the northeastern Tibetan Plateau

Understanding the geodynamics of plateau evolution requires examining the spatial and temporal aspects of mountain building in the northeastern Tibetan Plateau, which are still under debate. Here we integrate apatite geo-thermochronological and geochemical data from the Oligocene-Miocene succession of the Xunhua Basin to elucidate the evolution of the regional topography. The results suggest sediment provenance changes, at ca. 28, 20 and 12 Ma, indicating topographic growth of the West Qinling, Laji Shan, and Jishi Shan source areas, respectively. Our findings reveal Oligocene-Miocene stepwise deformation and middle Miocene stress reorganization within the northeastern Tibetan Plateau. We attribute this to Oligocene lithospheric foundering beneath the south-central Tibetan Plateau and the middle Miocene initiation of sinistral strike-slip faults due to the underthrusting of India and North China. This study highlights the roles of lithospheric removal and continental underthrusting in plateau growth, and the value of multi-proxy apatite analyses in provenance studies.

Northeastern Tibetan Plateau

Petrography and mineralogy of selected pre-Middle Jurassic basement rocks beneath the Atlantic and Gulf Coastal Plains in Florida

Florida is covered by flat-lying sedimentary strata of the Atlantic and Gulf Coastal Plains. These strata have accumulated since Middle Jurassic time. The pre-Middle Jurassic, or basement, rocks that underlie the Coastal Plain in Florida are known only from drill cores and cuttings recovered from a relatively small number of boreholes. This data report presents petrographic observations and the results of X-ray diffraction analyses of basement rocks from 18 boreholes across Florida in support of the identification, discrimination, and correlation of units for a subcrop geologic map of pre-Middle Jurassic rocks composing Florida’s sub-Coastal Plain geology.

Florida

Colored shaded-relief bathymetric and acoustic-backscatter maps of Jenkinson Lake with orthomosaic of the Sly Park Creek and Hazel Creek area, California

The Caldor Fire was ignited on August 14, 2021, and burned almost 222,000 acres (898 square kilometers) in forested terrain of the central and western Sierra Nevada, California. During the subsequent two months, the fire burned nearly all of Sly Park Creek watershed in El Dorado County. The El Dorado Irrigation District manages the water supply for the area using storage in Jenkinson Lake, a 1.6-kilometer- (1.0-mile-) wide and 3.6-kilometer- (2.2-mile-) long reservoir, located south of the town of Pollock Pines. Several weeks after the fire, the U.S. Geological Survey began investigations into post-fire landscape responses, including sediment yield, by measuring new sediment deposition in Jenkinson Lake. This study focused on the collection and processing of bathymetric and acoustic-backscatter data, as well as onshore aerial imagery in and around Jenkinson Lake, to support wildfire science after the Caldor Fire. A colored shaded-relief bathymetric map (sheet 1) and an acoustic backscatter map (sheet 2) show the lake floor morphology and backscatter intensities.

California

Integrating detrital magnetite geochemistry and (U-Th)/He chronometry as a sediment provenance tool in geologic and metallogenic terranes

Magnetite is ubiquitous in porphyry Cu systems and in sediment sourcing both barren and mineralized regions, with potential as an indicator mineral in concealed and coarsely-mapped terranes. We develop and test a workflow for integrated geochemistry and (U–Th)/He (He) dating for inferring detrital magnetite (DMt) provenance in these settings. The ca. 70 Ma Taurus porphyry Cu–Mo(–Au) district in eastern interior Alaska serves as a test case. DMt from streams draining porphyry-related mineralization was characterized by geochemistry and mineral inclusion and microstructure observations, complemented by similar data for potential porphyry and host rock sources. Principal component analysis and clustering of DMt geochemical data resolve multiple populations in our samples geochemically and texturally compatible with derivation from metamorphic, porphyry-related hydrothermal, and igneous sources. Hydrothermal magnetite comprises ∼16–50% of DMt nearest porphyry mineralization but diminishes to ∼4% ∼15 km downstream. Subsampled grains within populations yield ∼160–110 Ma, ∼70 Ma, ∼55 Ma, and ∼20 Ma magnetite He date modes. Combined with provenance, He dates capture Early Cretaceous regional exhumation of metamorphic host rock and Late Cretaceous porphyry Cu mineralization. DMt grains showing partial hematite replacement yield ca. 55–20 Ma dates regardless of source, overlapping regional warm/wet climatic intervals. We interpret Cenozoic dates to reflect exhumation to near-surface oxidizing conditions and(or) supergene weathering. Magnetite is thus a promising target phase for (1) tracking the spatiotemporal distribution of porphyry systems, and (2) linking the formation and exhumation of these systems to a regional geologic history, both in Alaska and globally.

Alaska, Yukon

Engineering and risk research—A strategic vision for extending USGS earthquake science to risk assessment and mitigation

As a part of the U.S. Geological Survey (USGS) Natural Hazards Mission Area, the Engineering and Risk Project (ERP) of the Geologic Hazards Science Center conducts civil engineering research to extend natural hazards science to risk assessments. The ERP includes predominantly, but not exclusively, staff funded by the USGS Earthquake Hazards Program. A major focus of the ERP is to deliver actionable information to decisionmakers that helps promote the safety, security, and economic well-being of the Nation. The report discusses briefly the ERP’s evolution and scope, its organization structure, describes key products and deliverables, their efforts to engage with external partners, and its strategy and vision in conducting range of engineering and risk research. The research topics outlined in this report, as developed by the ERP, may be used to facilitate project planning activities during the next 10 years and serve as a longer-term planning resource for the USGS Earthquake Hazards Program and Geologic Hazards Science Center.

Circular

Geologic map of the Sierra Nevada, California and western Nevada

THE GEOLOGIC MAP OF THE SIERRA NEVADA is a core component of the Sierra Nevada Earth Science Atlas, which also includes geophysical, neotectonic, economic, and geochronologic data. The map illustrates the distribution of geologic units across the Sierra Nevada and related adjacent areas. Geologic units are grouped by type and age into three categories: Principally Paleozoic and Mesozoic metasedimentary and metavolcanic wall rocks, most of which are grouped into terranes; Paleozoic and Mesozoic plutons and intrusive suites, which intrude the wall rocks and form the core batholith of the range; and Late Cretaceous and Cenozoic sedimentary and volcanic rocks and surficial deposits that unconformably overlap the older units. Related rock units were combined and simplified for presentation at a scale of 1:400,000, as shown in the list of map units and associated correlation of map units (Plate 1B) and description of map units (Appendix A). Tectonic faulting, folding and uplift have overprinted the rocks and have strongly influenced the spatial distribution of units and the distinct morphology of the Sierra Nevada as we see it today. The Atlas is the result of collaborative work by scientists and mapmakers from the California Geological Survey and the U.S. Geological Survey. The Atlas was originally envisioned by the late geologist Warren Nokleberg (1939-2021), who contributed much to the initial geologic map compilation

California, Nevada

Seasonal and hydrologic variation influences habitat and functional structure of stream fish assemblages

Introduction: Hydrologic variability is a key driver of ecological structure in lotic systems, shaping habitat conditions, taxonomic diversity, and the functional traits that mediate species’ persistence and performance (e.g., reproductive success). While many studies examine taxonomic responses to variation in flows, few evaluate how spatiotemporal hydrologic variation influences the functional organization within stream fish communities. Methods: We quantified seasonal habitat structure and functional trait diversity of fish assemblages across six Ozark Plateau headwater streams representing two contrasting flow regimes: Groundwater Flashy and Runoff/Intermittent Flashy. Fish and habitat data were collected seasonally during a dry year (2002) and a wet year (2003). Functional space was constructed using PCoA of morphological, ecological, and life-history traits, and functional diversity was measured using community weighted means (CWMs), functional richness (FRic), functional evenness (FEve), and functional divergence (FDiv). Results: We found that habitat structure differed strongly by flow regime and season, with Runoff/Intermittent streams exhibiting pronounced reductions in depth, area, and velocity, while groundwater streams remained structurally stable. Functional identity of assemblages was similar across flow regimes, dominated by benthic, hydrodynamic taxa with opportunistic and periodic life-history strategies. However, functional structure differed significantly: FEve and FDiv were consistently lower in Runoff/Intermittent Flashy streams in both years, indicating assemblage dominance of species with similar trait combinations and reduced trait partitioning under variable flow. FRic and taxonomic richness remained stable across seasons and flow regimes, suggesting high functional redundancy despite species turnover. Discussion: Together, results show that flow regime mediates both habitat structural stability and functional organization. As climatic warming and extreme drought increase hydrologic instability in headwaters, functional trait approaches provide a sensitive tool for detecting losses of functional roles that may not be evident by using taxonomic metrics alone.

Arkansas, Kansas, Missouri, Oklahoma

Water-quality assessment of south-central Texas: Occurrence and distribution of volatile organic compounds in surface water and ground water, 1983-94, and implications for future monitoring

The study area of the South-Central Texas study unit of the National Water-Quality Assessment Program comprises the Edwards aquifer in the San Antonio region and its catchment area. The first phase of the assessment includes evaluation of existing water-quality data for surface water and ground water, including volatile organic compounds, to determine the scope of planned monitoring. Most analyses of volatile organic compounds in surface water are from the National Pollutant Discharge Elimination System sites in San Antonio, Texas. Nine volatile organic compounds were detected at the six sites. The three compounds with the most detections at National Pollutant Discharge Elimination System sites are 1,2,4-trimethylbenzene, toluene, and xylene. Analysis of volatile organic compounds in ground water was limited to Edwards aquifer wells. Twenty-eight volatile organic compounds were detected in samples from 89 wells. The five most commonly detected compounds in samples from wells, in descending order, are tetrachloroethene, trichloroethene, bromoform, chloroform, and dibromochloromethane. Detections of volatile organic compounds in surface water and ground water within the South-Central Texas study area are limited to site-specific sources associated with development; therefore, planned monitoring for possible detections of volatile organic compounds as part of the National Water-Quality Assessment Program will emphasize areas of expanding population and development. Monitoring of volatile organic compounds is planned at National Pollutant Discharge Elimination System sites, at basic fixed surface-water sites, and in the ground-water study-unit surveys.

Texas

Groundwater and surface-water interactions in the Waihe‘e-Kahalu‘u watershed, O‘ahu, Hawai‘i—Analysis of historical data and numerical groundwater-model simulations

The Waihe‘e-Kahaluʻu watershed and surrounding areas lie in a dike-impounded groundwater setting of the Ko‘olau Range, O‘ahu, Hawai‘i, where groundwater withdrawal from wells and tunnels have resulted in reductions in streamflow. Resource managers and surface-water users seek information that can be used to balance the needs of competing uses of groundwater and streamflow in the watershed. This study assesses the effects of four groundwater developments in the Waihe‘e-Kahalu‘u watershed—Kahaluu Tunnel, Waihee Tunnel, Kahaluu Well, and Waihee Incline Wells—on Kahalu‘u and Waihe‘e Streams. Analyses of historical streamflow, withdrawal, and rainfall data indicate that base flow (the component of streamflow that is supplied from groundwater discharge) in Kahalu‘u and Waihe‘e Streams decreased in response to withdrawals from wells and tunnels within and outside the watershed. Groundwater-model simulations indicate that shutdown of selected tunnels and wells in the Waihe‘e-Kahaluʻu watershed will result in increases of groundwater discharge, but not all of the increase will occur in streams within the watershed; increases will also occur in streams outside the basin, the ocean, and tunnels other than those that were shut down. The analysis of historical data and model simulations is consistent in showing that the effects of withdrawal changes on streams are not constrained by the topographic boundaries of watersheds. Stream base flows in the Waihe‘e-Kahalu‘u watershed are subject to a complex interaction of multiple withdrawals from wells and tunnels installed at various times within and outside the watershed. The correspondence between withdrawals and base-flow changes is consistent with the conceptual model and understanding of the connection between groundwater and streamflow in the dike-impounded groundwater setting of the Waihe‘e-Kahalu‘u watershed and the Ko‘olau Range.

Hawaii

Decadal trends and occurrence of geogenic constituents and mixtures in groundwater across the continental United States

Worldwide, about 50% of the population is served by groundwater-sourced drinking water. Numerous groundwater quality assessments have found that geogenic constituents are among the most common contaminants in drinking-water aquifers. Documenting changing groundwater quality is a crucial aspect of water availability assessments. We assess trends and occurrence of geogenic constituent concentrations in groundwater across the continental United States using 3 decades of data from the U.S. Geological Survey’s National Water Quality Network. Thousands of groundwater wells were grouped into agricultural, urban, or domestic supply network types. Although most networks and constituents had no statistically significant change in concentration, many had increasing concentration trends, elevated concentrations, or both. Lithium, sodium, radium, sulfate, and uranium had increasing trends in more than 10% of the study networks. Urban and domestic well networks had increasing lithium and sodium trends more often than agricultural networks. Manganese most commonly increased in domestic well networks; uranium more commonly increased in agricultural and urban networks. Elevated concentration mixtures were widespread, and mixture complexities appeared to increase over time. Our results indicate that more than 2.3 million domestic-well users may be affected by elevated concentrations of one or more geogenic constituents.

continental United States

Cultural benefits knowledge and social learning in Elwha River dam removal and ecosystem decision-making

In a retrospective case study of the seminal Elwha River dam removal in Washington State, USA, we explore pathways through which ecosystem services (ES) knowledge can inform environmental decision-making. We specifically investigate how knowledge of cultural benefits linked to the Elwha River ecosystem was made available. Through interviews and historical document review we identify diverse forms of cultural benefits knowledge (i.e., how individuals or groups know the contributions of an ecosystem to their identities, experiences, and capabilities), including both knowledge in the form of practices – embodied or enacted forms of knowledge, and knowledge in the form of products – quantitative or written documentation. Recognizing and learning from the diverse forms in which benefits knowledges are conveyed can support more accurate and equitable consideration of the diverse values, well-beings, and human-nature relationships linked to ecosystems. With this case study, we illuminate pathways for meaningful consideration of multiple knowledge forms, and the plural ecosystem values they convey, with relevance for environmental decision processes in the Puget Sound and beyond.

Washington

Surficial geology and Quaternary tectonics of the Madison Valley and fault zone, Madison, Gallatin, and Beaverhead Counties, southwest Montana

The north-northwest-striking Madison fault is approximately 95 kilometers in length, lying at the confluence of the northeastern Basin and Range province and the Yellowstone tectonic parabola. The fault zone consists primarily of west-dipping normal faults that have east-dipping antithetic faults, which create the Madison Valley graben and several northeast-trending intrabasin faults. The Madison fault and associated sections discussed herein refer to the main west-dipping, range-bounding fault along the eastern side of the valley. Detailed geologic mapping (1:12,000 scale) of the entire fault zone and fault scarp profiling (total of 102 profiles) of the Madison fault reveal greater late Quaternary paleoseismic activity towards the south, including at least three paleoevents along the southern part of the fault that postdate Pinedale glaciation. Early to middle Holocene alluvial fans have vertical surface offsets that average between 2.0 and 3.0 meters and define the characteristic single-event surface offset. Pinedale lateral moraines have vertical surface offsets as great as 12.0 meters. Late Pleistocene to Holocene multiple-event fault scarps show little evidence of beveling, suggesting short seismic recurrence intervals and potential late Pleistocene and Holocene temporal clustering. Long-term average tectonic activity rates indicate slip rates ranging from 0.18–0.6 millimeters per year. Based on a comparison of fault-scarp height versus maximum slope angle of known regression lines developed from other paleoseismic investigations, the most recent event ranges from 5–1 ka. The northern section of the fault zone is defined by multiple normal faults, which detached the hanging walls of Laramide thrust faults within the Paleozoic and Mesozoic strata. This resulted in the partitioning of extension along multiple preexisting structures and less displacement along individual normal fault strands. Structural controls on lateral propagation of individual paleoevents involve the position of lateral ramps along preexisting Laramide contractional faults. This resulted in greater displacement within the larger basement-cored structures along the southern section of the fault zone, where extension is accommodated by one inferred principal basement-involved normal fault. Inferred east-northeast trending, intrabasin, normal faults within the southern half of the fault zone have no late Pleistocene displacement.

Idaho, Montana, Wyoming

Event classification, seismicity, and eruption forecasting at Great Sitkin Volcano, Alaska: 1999–2023

The frequency content of volcanogenic seismicity is often used to classify events and their spatial and temporal progression is then used to map subsurface volcanic processes. The progression of volcano-seismic events and associated source processes also plays a critical role in eruption forecasting. Here we develop and evaluate a computerized methodology for characterizing volcano-seismic event types using Frequency Index and Average Peak Frequency. We apply and test this technique at Great Sitkin Volcano, Alaska, classifying over 9000 hypocenters between 1999 and 2023. This 24-year time span covers periods of seismic quiescence, earthquake activity on nearby tectonic (bookshelf) faults, precursory unrest from 2016 to 2021, and the explosive onset in May 2021 of the ongoing effusive eruption. We use the spatial and temporal evolution of classified event types to map the active volcanic and tectonic processes, develop a conceptual model of the subsurface magmatic system, and perform a retrospective analysis of eruption forecasts at Great Sitkin Volcano between 2016 and the present. The classification and progression of hypocenters suggests the subsurface Great Sitkin Volcano magmatic system consists of a mid- to lower- crustal source zone between 10 and 40 km depth and an upper crustal magma storage area between −1 and 10 km depth (hypocenter depth is referenced to sea level and negative depths reflect height above sea level). The earliest precursors occurred in July 2016 and consisted of deep long-period and volcano-tectonic earthquakes at mid-crustal depths suggesting the subsequent unrest and eruption were triggered by a deeper intrusion of magma. This mid-crustal seismic activity was immediately followed by the onset upper-crustal long-period events and volcano-tectonic earthquakes VTs suggesting a strong linkage between the shallow and deeper portions of the magmatic system. The upper crustal area was likely capped by the 1974 lava dome until the magmatic explosion on May 26, 2021.

Alaska

Paleomagnetic correlation of surface and subsurface basalt flows in the central and southwestern part of the Idaho National Laboratory, Idaho

The U.S. Geological Survey, in cooperation with the U.S. Department of Energy, used paleomagnetic data from 22 coreholes to construct 3 fence diagrams of subsurface basalt flows in the southern part of the Idaho National Laboratory. These diagrams provide comprehensive descriptions of the horizontal and vertical distribution of basalt flows and sediment layers beneath the surface, aiding geological studies and contributing valuable data to numerical models of groundwater flow and contaminant transport. The correlations established though these diagrams include spatial correlations between basalt flows found in multiple coreholes. Correlations were identified by matching average paleomagnetic inclinations and confirming or denying these correlations using petrology, geochemistry and radiometric ages. The fence diagrams aid in identifying potential locations of subsurface vents, volcanic vents that have been buried by more recent volcanic activity, associated to subsurface basalt flows. By tracing the subsurface flows and analyzing where the greatest thickness occurs, the locations of buried vents can be inferred. Some subsurface flows exhibit correlations across several coreholes and may indicate yet unidentified surface or buried vents, thereby enhancing our understanding of the volcanic history and subsurface geology of the region.

Idaho

Geochemical geodatabase of sedimentary strata (coal, coal-adjacent rocks, tuffaceous oil shale, phosphate-rich rocks) and produced water in the Uinta region, Utah and Colorado

The Geochemical Geodatabase of Sedimentary Strata (Coal, Coal-adjacent Rocks, Tuffaceous Oil Shale, Phosphate-rich Rocks) and Produced Water in the Uinta Region, Utah and Colorado, consists of compiled datasets acquired as part of the Carbon Ore, Rare Earth, and Critical Mineral (CORE-CM) Uinta Region assessment funded by the U.S. Department of Energy (DEFE0032046, 2021–2024; Birgenheier et al., 2024). The CORE-CM assessment focused on providing comprehensive geological and geochemical characterization of current and prospective sedimentary-hosted resources including coal, oil shale, phosphatic limestone, and produced water from oil and gas targets present in eastern Utah and northwestern Colorado (Figure 1). This Data Series includes a geodatabase that consists of analytical geochemical data collected September 2021 through December 2024 via portable X-ray fluorescence (pXRF), and laboratory measured analyses produced by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectroscopy (ICP-OES). The coal-related geochemical data are derived primarily from the Cretaceous Blackhawk Formation and Ferron Sandstone of Utah, and the Mesaverde Group of Colorado. Additional non-coal resources assessed include oil shale-bearing strata of the Eocene upper Green River Formation (Utah and Colorado), phosphate-rich limestone of the Permian Park City Formation (Utah) and produced water from oil and gas-bearing strata of the Eocene Green River and Wasatch Formations (Uinta Basin) and the Pennsylvanian Paradox Formation (Paradox Basin) (Table 1). The CORE-CM assessment included a wide range of lithologies present in the coal, oil shale, and phosphate geologic resource systems whether or not the specific lithology has current economic value. Geochemical analyses of produced water from oil and gas wells focused on current and emerging hydrocarbon targets in the central Uinta Basin and northern Paradox Basin. A total of 13,092 geochemical analyses from these geologic systems is provided in the included geodatabase. A series of coal quality data (e.g., composition and maceral analyses) is also included in the database and was digitized from archived coal samples from the Utah Geological Survey (Appendix A).

Colorado, Utah

Timescales of surface faulting preservation in low-strain intraplate regions from landscape evolution modeling and the geomorphic and historical record

Large surface-rupturing intraplate earthquakes in stable continental regions (SCRs) are uncommon globally and have recurrence intervals of thousands to hundreds of thousands of years based on the paleoseismic and geomorphic record, challenging accurate active fault identification in these regions. To constrain the timescales of preservation for scarps created by surface ruptures from dip-slip earthquakes, we use a two-dimensional scarp diffusion model for typical intraplate settings and explore which parameters influence fault scarp preservation. These parameters include the coseismic vertical surface offset, the recurrence interval of similar magnitude earthquakes, diffusivity (as a proxy for mean annual precipitation rate), and the erodibility of the surficial material. We constrain parameter ranges from a compilation of historical surface ruptures in intraplate settings in a variety of climates, including the Central and Eastern United States, Australia, Europe, Central Asia (Mongolia, China), India, and West Africa. The timescales of scarp preservation from landscape evolution modeling agree well with observations of scarp preservation in low-strain SCR and intraplate tectonic settings, with some notable exceptions for Australian scarps. We find that the erodibility of the surficial material and earthquake recurrence interval have a stronger effect on the timescales of scarp preservation than diffusivity or coseismic vertical surface offset. Our model results may aid in identifying and characterizing subtle, slow-moving active faults in low-strain SCR and intraplate tectonic settings for different tectonic, geomorphic, and climatic characteristics. Accurate fault locations and characterization from the landscape record has implications for both probabilistic seismic and fault displacement hazard analyses.

Journal of Geophysical Research Solid Earth