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Variation in active and passive resource inputs to experimental pools: mechanisms and possible consequences for food webs

1. Cross-ecosystem movements of resources, including detritus, nutrients and living prey, can strongly influence food web dynamics in recipient habitats. Variation in resource inputs is thought to be driven by factors external to the recipient habitat (e.g. donor habitat productivity and boundary conditions). However, inputs of or by ‘active’ living resources may be strongly influenced by recipient habitat quality when organisms exhibit behavioural habitat selection when crossing ecosystem boundaries. 2. To examine whether behavioural responses to recipient habitat quality alter the relative inputs of ‘active’ living and ‘passive’ detrital resources to recipient food webs, we manipulated the presence of caged predatory fish and measured biomass, energy and organic content of inputs to outdoor experimental pools of adult aquatic insects, frog eggs, terrestrial plant matter and terrestrial arthropods. 3. Caged fish reduced the biomass, energy and organic matter donated to pools by tree frog eggs by ∼70%, but did not alter insect colonisation or passive allochthonous inputs of terrestrial arthropods and plant material. Terrestrial plant matter and adult aquatic insects provided the most energy and organic matter inputs to the pools (40–50%), while terrestrial arthropods provided the least (7%). Inputs of frog egg were relatively small but varied considerably among pools and over time (3%, range = 0–20%). Absolute and proportional amounts varied by input type. 4. Aquatic predators can strongly affect the magnitude of active, but not passive, inputs and that the effect of recipient habitat quality on active inputs is variable. Furthermore, some active inputs (i.e. aquatic insect colonists) can provide similar amounts of energy and organic matter as passive inputs of terrestrial plant matter, which are well known to be important. Because inputs differ in quality and the trophic level they subsidise, proportional changes in input type could have strong effects on recipient food webs. 5. Cross-ecosystem resource inputs have previously been characterised as donor-controlled. However, control by the recipient food web could lead to greater feedback between resource flow and consumer dynamics than has been appreciated so far.

Freshwater Biology

Growth, body composition and hepatic tyrosine aminotransferase activity in cortisol-fed channel catfish, Ictalurus punctatus Rafinesque

Metabolic consequences of chronic elevation of cortisol in the diet of yearling channel catfish, Ictalurus punctatus , were studied. Cortisol was incorporated into the diet in concentrations of 1, 10, 50 and 100 μg/g of food. This diet was offered at 3% of the body weight per day for 10 weeks. Fish were individually weighed and measured at 2-week intervals and feeding rates were adjusted. Body weight, liposomatic index and condition factor were significantly lower and food conversion was significantly higher in fish fed 50 or 100 μng cortisol/g of food when compared with controls. The hepatosomatic index of fish fed cortisol at the rate of 100 μg/g of food decreased significantly. Specific activity of hepatic tyrosine aminotransferase was significantly higher at the two highest cortisol doses. Long-term cortisol administration can reduce growth and condition factor by activating gluconeogenic mechanisms in which lipids and amino acids, rather than carbohydrates, are used for energy production. The metabolic effects of exogenous cortisol in this study offer an explanation for the decreased growth of fish under conditions that activate the secretion of endogenous cortisol.

Journal of Fish Biology

Effects of food supplementation on depredation of duck nests in prairies by striped skunks

We examined provision of supplemental food as a method for reducing depredation of upland-duck nests, especially by striped skunks (Mephitis mephitis). Predators negatively influence duck recruitment in grassland ecosystems. Managers are in search of methods, particularly nonlethal methods, for reducing nest depredation. We conducted this study on 24 areas managed for wildlife production in the Prairie Pothole Region of central North Dakota during 1993-1994. We provided a mix of fish offal and sunflower seeds on 12 areas; no food was provided on the remaining 12 control areas. Although we observed a tendency during both years for higher nest success rates on provisioned areas ( = 46%, 1993; 36%, 1994) than on control areas ( = 27%, 1993; 31%, 1994), mean nest success rates (Mayfield 1961) overall did not differ significantly between food-provisioned areas ( = 41%) and control areas ( = 29%). Striped skunk depredation rate was lower on food-provisioned areas (11%) than on control areas (24%), suggesting that skunks reduced their consumption of eggs when provided with a food supplement. In 1994, habitat conditions were optimal, and ducks nested persistently into the summer when nest success rates of food-provisioned areas and control areas differed by only 5 percentage points. That year American badgers (Taxidea taxus) and Franklin's ground squirrels (Spermophilus franklinii) apparently compensated for reduced depredation by skunks. Thus, although skunks and other mammalian predators seem to have responded positively to food provisioning, nest depredations overall did not change. Provision of supplemental food apparently has limited value for managing depredation of upland duck nests in the Prairie Pothole Region where predator communities are complex.

Wildlife Society Bulletin

Native and nonnative fish populations of the Colorado River are food limited--evidence from new food web analyses

Fish populations in the Colorado River downstream from Glen Canyon Dam appear to be limited by the availability of high-quality invertebrate prey. Midge and blackfly production is low and nonnative rainbow trout in Glen Canyon and native fishes in Grand Canyon consume virtually all of the midge and blackfly biomass that is produced annually. In Glen Canyon, the invertebrate assemblage is dominated by nonnative New Zealand mudsnails, the food web has a simple structure, and transfers of energy from the base of the web (algae) to the top of the web (rainbow trout) are inefficient. The food webs in Grand Canyon are more complex relative to Glen Canyon, because, on average, each species in the web is involved in more interactions and feeding connections. Based on theory and on studies from other ecosystems, the structure and organization of Grand Canyon food webs should make them more stable and less susceptible to large changes following perturbations of the flow regime relative to food webs in Glen Canyon. In support of this hypothesis, Grand Canyon food webs were much less affected by a 2008 controlled flood relative to the food web in Glen Canyon.

Colorado River

Experimental changes in food and ectoparasites affect dispersal timing in juvenile burrowing owls

Natal dispersal is a key demographic trait that affects population dynamics, and intraspecific variation in dispersal affects gene flow among populations and source-sink dynamics. However, relatively little is known about the selective pressures and trade-offs that animals face when departing their natal area due to the logistical difficulties associated with monitoring animals during this critical life stage. We used a randomized block design to examine the selective pressure that influence dispersal timing in juvenile burrowing owls ( Athene cunicularia ) by experimentally altering both food and ectoparasites at 135 nests. We also examined the effects of local food abundance, ectoparasite loads, and parental departure on natal dispersal timing. Juvenile burrowing owls varied widely in natal dispersal timing, and phenotypic plasticity in dispersal timing was evident in juvenile owls’ response to our experimental treatments, local conditions, and their parents’ departure from the natal area. Moreover, juveniles responded differently than their parents to experimental manipulation of food and ectoparasite loads. Juveniles typically dispersed shortly after their parents departed the natal area, but delayed dispersing more than 2 weeks after parental departure if they did not receive experimental food supplements during a low-food year. In contrast, the experimental food supplements did not affect the migratory departure decisions of adult owls in either year. Juveniles at nests treated for ectoparasites initiated dispersal at a younger age (and prior to adults in the high-food year) compared to juveniles at control nests. In contrast, parents at nests treated for ectoparasites departed later than parents at control nests. Our results suggest that unfavorable conditions (low food or high ectoparasite loads) caused juveniles to delay dispersal, but prompted adults to depart sooner. Our results highlight the extent of intraspecific variation in natal dispersal timing, and demonstrate that ecological conditions affect dispersal decisions of parents and offspring differently, which can create important trade-offs that likely affect life history strategies and responses to climatic changes.

Washington

Save the Great Lakes fisheries!

During the winters of 1939-40 and 1940-41, two experiments involving 456 bobwhite quail, were conducted at the Patuxent Research Refuge, Bowie, Maryland, to determine the palatability and comparative feeding value of 20 common wild quail-foods when offered as a part of a maintenance diet....2. Mortality was low on all diets, the highest occurring on that containing a mixture of bayberry and wax myrtle fruits. On all diets the birds showed increases in weight during the early winter. These gains were better maintained by birds on the wild foods than those on control diets composed entirely of cultivated feedstuffs.....3. There were statistically significant differences between the quantities of the various diets eaten.....4. For palatability and acceptability, seeds of common ragweed (Ambrosia artemisiifolia) and of shrub lespedeza (Lespedeza bicolor) were the preferred wild foods.....5. It is concluded that pen-reared bobwhite quail can be maintained successfully through winters in the vicinity of Washington, D. C., on diets containing 50 per cent of any one of the wild foods listed and 50 per cent of a high-caloric commercial seed mixture with yellow corn as the principal cereal.....6. The results of these 'cafeteria' studies, although interesting and indicative of the preferences of quail in confinement, do not necessarily represent selections that might be. made by bobwhites in the wild.

Outdoor American

Reduction of provisioning effort in response to experimental manipulation of chick nutritional status in the Horned Puffin

Using a supplemental feeding experiment, we investigated the ability of adult Horned Puffins to decrease provisioning effort in response to reduced nutritional requirements of chicks. We found no difference between experimental and control groups in parental provisioning before supplementary feeding was initiated. After receiving supplemental food for seven days, experimental chicks grew faster, gained more mass and received 87% less food from their parents than did control chicks. These results demonstrate that Horned Puffin parents can decrease food provisioning in response to a decrease in their chick nutritional requirements.

Alaska

Impacts of upper respiratory tract disease on olfactory behavior of the Mojave desert tortoise

Upper respiratory tract disease (URTD) caused by Mycoplasma agassizii is considered a threat to desert tortoise populations that should be addressed as part of the recovery of the species. Clinical signs can be intermittent and include serous or mucoid nasal discharge and respiratory difficulty when nares are occluded. This nasal congestion may result in a loss of the olfactory sense. Turtles are known to use olfaction to identify food items, predators, and conspecifics; therefore, it is likely that URTD affects not only their physical well-being but also their behavior and ability to perform necessary functions in the wild. To determine more specifically the impact nasal discharge might have on free-ranging tortoises ( Gopherus agassizii ), we compared the responses of tortoises with and without nasal discharge and both positive and negative for M. agassizii antibodies to a visually hidden olfactory food stimulus and an empty control. We found that nasal discharge did reduce sense of smell and hence the ability to locate food. Our study also showed that moderate chronic nasal discharge in the absence of other clinical signs did not affect appetite in desert tortoises.

Journal of Wildlife Diseases

Latitudinal variation in avian incubation attentiveness and a test of the food limitation hypothesis

Avian incubation attentiveness has important fitness consequences through its influence on the number and quality of hatched young and energetic costs imposed on parents. Nest attentiveness is highly variable across species and geographical regions. We reviewed the literature and found a worldwide pattern that nest attentiveness of passerines is generally lower in south temperate and tropical regions than in north temperate regions. We also conducted a food manipulation experiment to assess the extent to which nest attentiveness may reflect proximate responses versus an evolved behaviour. We used the karoo prinia, Prinia maculosa, in South Africa, which has very low nest attentiveness (???49%) compared with that of many passerine birds. We provided supplemental food during early incubation to experimental females and compared nest attentiveness and on- and off-bout lengths of experimental and paired control females.??Nest attentiveness of females at food-provisioned nests was significantly higher than that of control females (57% versus 49%). Food-supplemented females also spent significantly less time off the nest than did control females, whereas mean on-bout lengths did not differ. However, mean nest attentiveness of food-provisioned females was still substantially below that of other similar bird species worldwide. Food can be an important proximate influence on parental care behaviour, but proximate influences of food do not explain broad latitudinal patterns of attentiveness. ?? 2007 The Association for the Study of Animal Behaviour.

Animal Behaviour

Physical and ecologic features of the Sagadahoc Bay Tidal Flat, Georgetown, Maine

Sagadahoc Bay is open to the ocean at the south and has no significant fresh-water stream entering it. The intertidal zone is roughly a mile long by half a mile wide; most of it is made up of medium to fine sand, but organic-rich mud characterizes the head of the flat and the protected coves. Refraction-seismograph surveys showed that the bedrock surface lies 30 to 200 feet below the surface of the tidal flat and that it is irregular and fluted longitudinally. Repeated surveys indicate that the intertidal flat builds up and cuts down but is apparently in equilibrium with the present sea level. The sediment that fills the bay came from the sea. Waves and tidal currents tend to move it landward; storms accelerate this, or reverse the direction of movement, depending on the characteristics of the storm. Tidal- and wave-generated currents 0.1 foot above the bottom range in velocity from 0.35 to 0.82 foot per second on incoming tides and from 0.20 to 0.58 foot per second on ebbing tides. Incoming tides float large quantities of sand landward; ebbing tides never carry floating sand. Two distinctive and extensive Mya shell-pavement layers were found at depths of roughly 2 and 3 feet below the present surface of the tidal flat. The lower layer is approximately 1,000 years old according to a radiocarbon age determination of its Mya shells. It is suggested that these shell layers formed by sluicing away of a layer of sand about 2 feet thick, which had been thrown into loose packing by an earthquake at high tide. The earth shock induced a submarine slide of the sand in the outer part of the bay, which oversteepened the profile of the sand headward nearly to the head of the bay. Mya and other shells settled through the layer of quicksand while the sand was running out seaward. Living in the intertidal zone is the usual assemblage of clams, gastropods, crustaceans, worms, and seaweeds found on most northern New England tidal flats. The Mya arenaria population is decreasing, but in general myas are more numerous in the muddy areas than in the sandy areas. They grow more rapidly in the sand, though in the past decade there has been no significant renewal of the Mya population in the sandy part of the flat. Macoma balthica inhabits the muddy areas, whereas Ensis, Spisula, and Arctica are restricted to the low-tide zone and the shallow water below. Gemma gemma grows in great abundance in the sandy part of the flat but is rare in the muddy parts. Small shrimp and green crabs are common. The calcareous shells of these animals are all potential fossils, but the shrimp and crab exoskeletons are not, for their tests are rapidly decomposed in this environment. Other potential fossils are wood and bark, acorn caps, conifer cones, leaves of deciduous trees, seeds, and occasionally even grass stems and pieces of eel grass. All these are reasonably well preserved in the constant reducing environment that prevails an inch or two below the surface. An inverse relationship exists between the abundances of Mya arenaria and Gemma gemma. Cores and test pits show that gemmas are more numerous on the Sagadahoc flat now than they have been in the recent past (estimated 10–100 years). Gemmas are the dominant mollusk in the sandy part of the flat now that the myas are so extremely rare. The speculation is that gemmas became dominant largely because the Mya population was greatly reduced by intensive digging during and just after the last war and through depredations by green crabs. Possibly the warming climate has favored the gemmas selectively. The writer infers that the gemmas are a serious competitor of the myas and that the gemmas now starve out Mya spat, which is known to be carried into the bay each spring and fall. Two recommendations are made: (1) determining under controlled laboratory conditions the food requirements of Gemma and the density of Gemma population that will permit survival of Mya larvae from set through a stage that will assure maturation; and (2) killing off a large percentage of the Gemma population and observing whether or not a natural set of Mya occurs. Gemmas can be killed quickly under a flame shield such as is used to soften asphalt-sand mixtures in street paving. Inasmuch as gemmas are ovoviviparous they should not repopulate the flat rapidly.

Maine

Blue tilapia (Oreochromis aureus) predation on fishes in the Muddy River system, Clark County, Nevada

Blue tilapia (Oreochromis aureus), native to North Africa and the Middle East (Courtenay and Robins 1973, Fuller et al. 1999), has been introduced around the world as a human food source, for vegetation control, and as a game fish (Costa-Pierce and Riedel 2000). Blue tilapia has been particularly successful in establishing and spreading in North American waters where it has been reported to change fish community structure and cause native fish decline (Courtenay and Robins 1973, Fuller et al. 1999). Because of these detrimental effects, it is now generally considered an unwelcome introduction into North American waters (Dill and Cordone 1997, Fuller et al. 1999).

Western North American Naturalist

Attwater's prairie-chicken-its life history and management

Attwater's prairie chicken, a characteristic bird of the Texas coastal prairie, is closely related to the now extinct heath-hen of northeastern North America. Once abundant in an area extending from the coastal tall-grass prairies of southwestern Louisiana and Texas west and south to near Port Isabel, it has decreased in numbers as man has exploited its habitat, until now it is threatened with the same fate as that of the heath-hen. Important factors limiting the numbers of the bird include excessive or persistent rainfall during the nesting season, heavy grazing, excessive pasture burning, agricultural operations, and overshooting. Management will usually involve protection from excessive killing, improvement of food and cover, and control of predators and of the kill by hunters. Responsibility for this rests with the landowner. Optimum prairie chicken range apparently consists of well-drained grassland, with some weeds or shrubs, the cover varying in density from light to heavy; and with surface water available in summer; diversification within the grassland type is essential. In the absence of ample refuges for the species, probably all other favorable factors together will fail to save Attwater's prairie chicken from extinction. This number continues the series of the North American Fauna issued by the Bureau of Biological Survey, of the United States Department of Agriculture, prior to its transfer and consolidation with the Bureau of Fisheries on June 30, 1940, to form the Fish and Wildlife Service, in the Department of the Interior.

North American Fauna

Experimental landscape reduction of wild rodents increases movements in the invasive brown treesnake (Boiga irregularis)

Experimental studies evaluating the effects of food availability on the movement of free-ranging animals generally involve food supplementation rather than suppression. Both approaches can yield similar insights, but we were interested in the potential for using food suppression for the management and control of invasive predators, in particular, the brown treesnake ( Boiga irregularis ) on Guam. However, understanding a species’ response to food resources is critical before employing such a strategy. We studied the movements of 24 radio-tagged B. irregularis initially caught within four 4-ha unfenced plots in rodent-abundant (control) and rodent-suppressed (treatment) grassland habitats over a 40-day period. Because monitoring duration differed among snakes, we also analyzed short-term (16-day) activity areas. Over the 16 days, snakes associated with rodent-suppressed plots had 86% larger activity areas (ha), 94% greater dispersal distances (m), and 43% greater movement rates (m/h) than snakes associated with control plots. Boiga irregularis moved extensively, but these movements were not always reflected in the size of the snake’s total activity area. Movement rates did not differ between sexes, but snakes in above-average body condition moved greater distances per hour than those in below-average condition irrespective of treatment. Our study indicates that a relatively small prey suppression effort can cause almost immediate and significant changes in B. irregularis movement. On Guam, prey suppression might enhance control efforts by either increasing trap capture success or discouraging snakes from entering areas of conservation or management concern. However, the outcome of using prey suppression as a control tool in areas threatened with the accidental introduction of the brown treesnake is more difficult to predict and might have negative consequences such as elevated dispersal rates.

Management of Biological Invasions

Mercury in wetlands at the Glacial Ridge National Wildlife Refuge, northwestern Minnesota, 2007-9

The Glacial Ridge National Wildlife Refuge was established in 2004 on land in northwestern Minnesota that had previously undergone extensive wetland and prairie restorations. About 7,000 acres of drained wetlands were restored to their original hydrologic function and aquatic ecosystem. During 2007–9, the U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service and the Red Lake Watershed District, analyzed mercury concentrations in wetland water and sediment to evaluate the effect of wetland restoration on mercury methylation. The wetland waters sampled generally were of the calcium/magnesium bicarbonate type. Nitrogen in water was mostly in the form of dissolved-organic nitrogen, with very low dissolved-nitrate and dissolved-ammonia concentrations. About 71 percent of all phosphorus in water was dissolved, with one-half of that in the form of orthophosphorus. Wetland water had total-mercury and methylmercury concentrations ranging from 1.5 to 20 nanograms per liter (ng/L) and 0.2 to 16 ng/L, respectively. Median concentrations were 7.1 and 2.9 ng/L, respectively. About one-half of the mercury in wetland water samples was in the form of methylmercury, but this form ranged from 7 to 81 percent of each sample. Compared to concentrations in stream sediment samples collected throughout the United States, Glacial Ridge National Wildlife Refuge wetland sediment samples contained typical total-mercury concentrations, but methylmercury concentrations were nearly twice as high. The maximum concentration measured in Glacial Ridge National Wildlife Refuge wetland water approached the highest published water methylmercury concentration in uncontaminated waters of which we are aware. However, the upper quartile of water methylmercury concentrations is similar to concentrations reported for some impoundments and wetlands in northwestern Minnesota and North Dakota. Methylmercury concentrations in sampled wetlands were much higher than those from typical lakes or flowing streams throughout the United States. The high concentrations of methylmercury measured in sampled wetlands indicate the potential for substantial methylmercury concentrations in aquatic biota and wildlife that consume those biota. These wetlands also are a methylmercury source for downstream lakes and rivers. The high concentrations of methylmercury in water, its bioaccumulation potential, and its known toxicity in aquatic birds and food webs highlight a need to assess methylmercury in the biota within these ecosystems. Better understanding of factors that control methylmercury production concentrations within aquatic food webs in ecosystems of the Glacial Ridge National Wildlife Refuge would enable resource managers to better understand and manage risk to wildlife.

Minnesota

Linking ecosystems, food webs, and fish production: subsidies in salmonid watersheds

Physical characteristics of riverine habitats, such as large wood abundance, pool geometry and abundance, riparian vegetation cover, and surface flow conditions, have traditionally been thought to constrain fish production in these ecosystems. Conversely, the role of food resources (quantity and quality) in controlling fish production has received far less attention and consideration, though they can also be key productivity drivers. Traditional freshwater food web illustrations have typically conveyed the notion that most fish food is produced within the local aquatic habitat itself, but the concepts and model we synthesize in this article show that most fish food comes from external or very distant sources—including subsidies from marine systems borne from adult returns of anadromous fishes, from fishless headwater tributaries that transport prey to downstream fish, and from adjacent streamside vegetation and associated habitats. The model we propose further illustrates how key trophic pathways and food sources vary through time and space throughout watersheds. Insights into how food supplies affect fishes can help guide how we view riverine ecosystems, their structure and function, their interactions with marine and terrestrial systems, and how we manage natural resources, including fish, riparian habitats, and forests.

Fisheries

Melanin-based color of plumage: role of condition and of feathers' microstructure

Whether melanin-based colors honestly signal a bird's condition during the growth of feathers is controversial, and it is unclear if or how the physiological processes underlying melanogenesis or color-imparting structural feather microstructure may be adversely affected by condition. Here we report results from two experiments designed to measure the effect of condition on expression of eumelanic and pheomelanic coloration in black-capped chickadees (Poecile atricapillus) and zebra finches (Taeniopygia guttata), respectively. In chickadees, we compared feathers of birds affected and unaffected by avian keratin disorder, while in zebra finches we compared feathers of controls with feathers of those subjected to an unpredictable food supply during development. In both cases we found that control birds had brighter feathers (higher total reflectance) and more barbules, but similar densities of melanosomes. In addition, the microstructure of the feathers explained variation in color more strongly than did melanosome density. Together, these results suggest that melanin-based coloration may in part be condition-dependent, but that this may be driven by changes in keratin and feather development, rather than melanogenesis itself. Researchers should be cautious when assigning variation in melanin-based color to melanin alone and microstructure of the feather should be taken into account.

Integrative and Comparative Biology

Behavior, size, and body condition predict susceptibility to management and reflect post-treatment frequency shifts in an invasive snake

Foraging behavior can have population-level effects that are of interest for wildlife management. For invasive species, foraging behavior has been tied to establishment ability and rate of spread and is generally of import in understanding invasion biology. A major method for controlling invasive vertebrates is using food-based baits as attractants. Tool efficacy is therefore partially driven by individual decision-making during foraging, which may also affect population response to control. We used three studies on the invasive, arboreal brown treesnake ( Boiga irregularis ) on Guam to measure 1) size, body condition, and behavioral correlates with mortality in response to control using toxic baits, 2) shifts in prevalence of those traits after control treatments occurred and, based on the prior two findings, 3) interactive relationships between size, body condition, and behavioral traits at the landscape scale for untreated populations. Each trait was selected due to a potential relationship with foraging behavior or energetic state of an arboreal snake, as a method to estimate how foraging behavior can inform control tool efficacy. We found that snakes were more likely to be killed by toxiic baits if they had a lower body weight, were more active leading up to a bait application, and encountered on the ground more frequently. Across two treated populations, both body size and condition of sampled snakes increased after treatment, while the incidence of ground encounters decreased. Throughout forested habitat on Guam, ground encounter probability was positively correlated with snake size and inversely correlated to body condition. Additionally, size and condition had interactive effects such that snakes in good condition were more arboreal. Thus, body size and behavior (ground encounters) correlated with control-tool susceptibility and frequency shifts occurred in those traits within sampled post-treatment populations. Individual decision-making during foraging may thus inform population responses to bait-based control tools. Management decisions such as prey suppression are likely to directly influence removal efficacy through altered foraging behavior in snakes.

Global Ecology and Conservatuin

Evidence for rapid gut clearance of microplastic polyester fibers fed to Chinook Salmon: A tank study

Marine and freshwater plastic pollution is a challenging issue receiving large amounts of research and media attention. Yet, few studies have documented the impact of microplastic ingestion to aquatic organisms. In the Pacific Northwest, Chinook salmon are a culturally and commercially significant fish species. The presence of marine and freshwater microplastic pollution is well documented in Chinook salmon habitat, yet no research has investigated the impacts to salmon from microplastic ingestion. The majority of the marine microplastics found in the Salish Sea are microfibers, synthetic extruded polymers that come from commonly worn clothing. To understand the potential impacts of microfiber ingestion to fish, we ran a feeding experiment with juvenile Chinook salmon to determine if ingested fibers are retained or digestion rates altered over a 10 day digestion period. The experiment was completed in two trials, each consisted of 20 control and 20 treatment fish. Treatment fish were each fed an amended ration of 12 food pellets spiked with 20 polyester microfibers and control fish were fed the same ration without added microfibers. Fish were sampled at day 0, 3, 5, 7, and 10 to assess if fibers were retained in their gastrointestinal tract and to determine the rate of digestion. Fibers for the experiment came from washing a red polyester fleece jacket in a microfiber retention bag. Fibers had a mean length of 4.98 mm. Results showed fish were able to clear up to 94% of fed fibers over 10 days. Differences in mean gastrointestinal mass were not statistically significant at any sampled time between treatment and controls, suggesting that the ingestion of microfibers did not alter digestion rates. Further work is needed to understand if repeated exposures, expected in the environment, alter digestion or food assimilation for growth.

Environmental Pollution