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Inset groundwater-flow models for the Cache and Grand Prairie Critical Groundwater Areas, northeastern Arkansas

The water resources in the Mississippi alluvial plain, located in parts of Missouri, Kentucky, Tennessee, Mississippi, Louisiana, and Arkansas, supports a multibillion-dollar agricultural industry that relies heavily on pumping of groundwater for irrigation of crops and aquaculture. The primary source of groundwater for agricultural-related pumping is the Mississippi River Valley alluvial aquifer, which has declined in storage for decades; secondary groundwater sources include the middle Claiborne aquifer and Wilcox aquifer system. Two areas in northeastern Arkansas that lie within the Mississippi alluvial plain, part of the Cache and Grand Prairie regions, have been designated as Critical Groundwater Areas owing to decades of groundwater declines that resulted from past and current water use. The multidisciplinary Mississippi Alluvial Plain project, led by the U.S. Geological Survey, and funded by their Water Availability and Use Science Program, included objectives to develop numerical groundwater models in focus regions, including the part of the Cache and Grand Prairie regions of northeastern Arkansas. Two inset models were developed using the child model capabilities of MODFLOW 6, the U.S. Geological Survey’s Modular Hydrologic Model simulation software. Both models, called the Cache model and Grand Prairie model, simulated the groundwater system and surface-water/groundwater interactions for the Mississippi River Valley alluvial aquifer and underlying Tertiary-age aquifers and confining units to the Midway confining unit. Each model was spatially discretized into 500-meter x 500-meter orthogonal cells on a grid with 5-meter constant-thickness vertical layers that represented the Mississippi River Valley alluvial aquifer and increasing thickness layers for the aquifers and confining units below the alluvial aquifer. The Cache and Grand Prairie models were calibrated with the PEST++ iterative ensemble smoother Version 5 and employed high dimensional parameterization schemes of 13,740 and 30,436 parameters, respectively. The Cache mean absolute residual for groundwater-level observations within each model domain for the priority well was 1.58 meters. Grand Prairie mean absolute residuals for the alluvial aquifer and middle Claiborne aquifer groundwater-level observations were 2.71 and 10.78 meters, respectively. The groundwater budgets for the Cache and Grand Prairie models were characterized by substantial outflows to irrigation wells, which constituted about 52 and 54 percent of all outflows, with the primary source of water to those wells being releases from unconfined aquifer storage.

Arkansas

Benthic habitat map of Olowalu Reef, Maui, Hawaii—Geomorphological structure, biological cover, and geologic zonation determined with spectral, lidar, and acoustic data

The fringing coral reef off Olowalu, Maui, Hawaii, has been identified as a local conservation priority site. In 2007, the National Oceanic and Atmospheric Administration (NOAA) produced a benthic habitat map of the Hawaiian Islands that was used as a foundation for this study. To support place-based management of the reef in the future, the U.S. Geological Survey (USGS) mapped the geologic zone, major and dominant geomorphological structure, biological cover type, and percent of biological cover for 11 square kilometers (km 2 ) of Olowalu Reef at a minimum mapping unit (MMU) of 100 square meters (m 2 ) to create a benthic habitat map. Heads-up digitization was employed on 0.50-meter (m) natural color satellite orthoimagery with ancillary 1-m acoustic backscatter imagery from single-scan sonar (sound navigation and ranging). A 1-m, 4-m, and 8-m digital bathymetric model (DBM) was interpolated from bathymetric lidar (light detection and ranging), and various geomorphometric layers derived from the DBMs were used for habitat interpretation. Still-frame imagery of the seafloor extracted from vessel-towed underwater video transects on Olowalu Reef served as ground validation points ( n =870) during active mapping and accuracy assessment points ( n =216) for thematic accuracy assessment. Thematic accuracy was cross-validated by the Hawai‘i Department of Land and Natural Resources Division of Aquatic Resources. Final thematic accuracy was 88.8 percent for major structure, 85.6 percent for dominant structure, 86.0 percent for major biological cover, and 78.6 percent for type and percent of major biological cover. Reef and hardbottom constituted 52 percent of the total mapped habitat, comprising mostly aggregate reef (31 percent) and pavement (11 percent), with large swaths of spur-and-groove (9 percent). Of this hardbottom, 17 percent was covered with moderate (10 to <50 percent) coral and 27 percent with high coral cover (50 to <90 percent). High (50 to <90 percent) macroalgae cover dominated the continuous sand sheets in offshore bank/shelf zones. The map created in this study supplements the NOAA 2007 map and expands on the observations made by USGS sampling of the reef. The NOAA 2007 map and our map differed in total areal extent by a negligible 6 m 2 and were in general thematic agreement. Our map is intended to serve as a baseline for public access, general research, local-level management, and reef change for future studies.

Hawaii

Arctic fold-and-thrust belts

The modern Arctic has been formed through a series of continent–continent collisions, accretion of terranes and phases of crustal extension. The Neoproterozoic Timanian, Paleozoic Caledonian and Uralian, and late Mesozoic Verkhoyansk–Kolyma, Chukotkan and Brookian orogenies formed several large fold-and-thrust belts (FTBs). The FTBs are exposed across vast areas of continents and continue offshore to form a complex tectonic basement for thick sedimentary basins, playing an important role in the history of accumulation and deformation of younger unmetamorphosed sedimentary successions that are the subject of this volume. Recognition of the importance of FTBs in the Arctic geological history and their role as a controlling factor of development of Arctic sedimentary basins resulted in this chapter, in which we review the current state of knowledge about Arctic FTBs and highlight questions that remain to be addressed. Enclosure D , a map showing boundaries of the FTB and their internal first-order structural fabric, is a part of the overview.

Book chapter

Tracing fluid evolution during batholith and deposit formation: In-situ and bulk rock Mo isotopes from the Questa porphyry Mo deposit, New Mexico, USA

The Oligocene Questa porphyry Mo deposit, located in New Mexico, USA, is a well-characterized system that formed over 400 kyr, punctuating the 8 Myr formation of the Questa-Latir batholith. Detailed sampling of the intrusions and minerals associated with magmatichydrothermal mineralization, as well as bookending barren intrusions, allows for examination of the Mo isotope record of the deposit and its host batholith. Through this established chronologic framework, such data can yield insights into the metallogenesis of deposit formation and long-term magma genesis evolution for upper crustal magmatism. The Mo isotopic compositions of barren intrusions yield a younging trend towards lighter δ 98 Mo. However, intrusions associated with Mo deposit formation yield heavier δ 98 Mo relative to barren magmatism, by up to 2.0‰. This indicates that they were affected by an isotopically heavy and Mo-rich fluid. The in-situ data from molybdenite covers the range observed in mineralized bulk rocks. However, individual samples consistently yield Mo isotopic variations of 0.4‰ to 0.5‰. The consistency of the δ 98 Mo variation in in-situ analyses of molybdenite within hand samples and individual mineral grains is likely the result of MoS 2 crystallization in a closed system and reflects an almost complete precipitation of Mo.

New Mexico

Groundwater structures fish growth and production across a riverscape

Landscapes are composed of habitat patches and conditions that vary across space and time. While habitat variability and complexity can support important ecological processes and ecosystem services, the dynamic nature of habitats can also constrain organismal growth and production as optimal conditions are fleeting. In riverine ecosystems, groundwater discharge to streams stabilises water temperature and flow regimes, thus mediating how habitat complexity is expressed. Yet, how stable habitats structure growth and production within the broader landscape matrix is not well understood. In this study, we explored the effects of groundwater on spatiotemporal variation in growth and production for juvenile Yellowstone cutthroat trout ( Oncorhynchus virginalis bouvieri ) across the upper Snake River catchment, Wyoming, USA. We combined machine learning techniques and remotely sensed landscape data to estimate groundwater availability across the river network, which we linked to stream temperature regimes and conspecific density. We then used Bayesian hierarchical models to quantify the effects of temperature, density and groundwater on spatiotemporal variation in fish growth and production in 52 focal reaches. Finally, we predicted body size trajectories and trends in total production continuously over both space and time to understand the effect of groundwater at the riverscape scale. Groundwater discharged to streams where topography changes abruptly in valley-bottom areas underlain by coarse glacial deposits. Groundwater stabilised temperature regimes and was associated with high trout densities. Temperature and density, in turn, interacted to influence growth rates: growth increased strongly with temperature, but this effect was reduced when density was high. Accordingly, variation in groundwater availability among stream reaches diversified growth and production regimes. In reaches with low groundwater availability, growth and production declined over time from summer maxima. In contrast, in reaches with high groundwater availability, temporal trends in growth and production were hump-shaped—peaking in autumn—and mean production was greater. At the riverscape scale, temporal asynchrony in growth rates generated convergent spatial variation in growth capacity, but—when combined with density—led to the formation of distinct hotspots of production. Our results demonstrate how groundwater, an important driver of aquatic ecosystem heterogeneity, structures trout growth and production across space and time. Importantly, rare, but stable habitats may disproportionately affect ecological processes and serve as key sources of population diversity at larger spatial scales.

Wyoming

StreamStats—A quarter century of delivering web-based geospatial and hydrologic information to the public, and lessons learned

StreamStats is a U.S. Geological Survey (USGS) web application that provides streamflow statistics, such as the 1-percent annual exceedance probability peak flow, the mean flow, and the 7-day, 10-year low flow, to the public through a map-based user interface. These statistics are used in many ways, such as in the design of roads, bridges, and other structures; in delineation of floodplains for land-use zoning and setting of insurance rates; for regulatory purposes, such as the permitting of wastewater discharges; and for hydrologic and climate change studies. StreamStats was first developed for Massachusetts and released in 2001. The application provided users with the ability to obtain streamflow statistics computed from data collected at USGS streamgages and to obtain estimates of streamflow statistics for user-selected ungaged sites. Massachusetts StreamStats used geographic information system software and digital mapping to compute drainage-basin characteristics, which were then used in statistical models to estimate streamflow statistics for the user-selected sites. The statistical models were in the form of equations that were developed through a process known as regression analysis. StreamStats was the first known web application with the ability to do interactive geoprocessing. The utility of Massachusetts StreamStats was instantly apparent, leading the USGS to develop a version of StreamStats that could be implemented nationally. USGS State offices normally were required to develop custom regression equations and prepare local digital mapping data needed for implementing StreamStats for their States. Funding needed to complete this work usually was provided through cooperative agreements between the USGS and State agencies. In 2004, Idaho became the first to be released in the national version of StreamStats. By 2023, 44 States were fully implemented and six were undergoing implementation. StreamStats has undergone many modifications over the years to keep up with changes to the underlying software and to add functionality. Customized functionality and separate linked StreamStats applications were developed for several States. Meeting the high demand for additions and improvements to StreamStats while also adhering to budgetary constraints has, at times, been challenging. The StreamStats development team has identified numerous additional improvements that could be made to provide better performance and more functionality. The lessons learned from the experience of building and operating StreamStats for nearly 25 years could be relevant to others interested in pursuing efforts of a similar scale.

Circular

Shallow geologic framework of the Mississippi Sound and the potential for sediment resources

The Mississippi Sound, an estuarine environment located between the mainland and barrier islands bordering the northern Gulf of America (formerly the Gulf of Mexico), serves as a vital ecosystem for the States of Mississippi and Alabama. Spanning approximately 100 kilometers from east to west and covering 1,400 square kilometers, the sound is home to marine industry and ports, and its shallow and brackish waters sustain a diverse array of marine life. Barrier islands along the southern edge of the sound separate the microtidal estuary from the Gulf of America. This protection from gulf wave action mediates current flow within the sound, resulting in predominantly fine-grained sediment deposition along the seafloor. This study, conducted by the U.S. Geological Survey in cooperation with the U.S. Army Corps of Engineers, provides insight on fluvial and tidal processes spanning the past 5,000 years. The report synthesizes existing research to provide a comprehensive overview of the sound geology, from Pleistocene origins to present-day morphology, and utilizes high-resolution single channel seismic profiles and sediment data to identify and map sedimentary deposits and morphologic features at and below the seafloor. Despite its ecological significance, the Mississippi Sound faces environmental challenges, including water-quality issues, habitat degradation, storm-induced erosion, and the ongoing threats of sea-level rise and environmental changes. This study uses the present-day understanding of the sound's geology to inform coastal management decisions, hazard assessment, and potential mineral resources.

Louisiana, Mississippi

Low flows and flow duration of Tennessee streams through 1981

Estimates of low-flow characteristics and flow duration for the period of record at continuous-record streamflow gages are essential in hydrologic studies and water-resources management. This report provides estimates of low flow for 1, 3, 7, 14, 30, 60, and 90 consecutive days for recurrence intervals of 2, 5, 10, and 20 years for continuous-record streamflow gages. These estimates were used in correlation methods to estimate low flow at partial-record streamflow sites for 1, 3, and 7 consecutive days for a recurrence interval of 10 years; and 3 consecutive days for a recurrence interval of 20 years.

Tennessee

System characterization report on the Environmental Mapping and Analysis Program (EnMAP)

This report addresses system characterization of the Environmental Mapping and Analysis Program hyperspectral sensor by the DLR (German Aerospace Center, ground segment project management), GFZ (Deutsches Geoforschungszentrum, science lead) and is part of a series of system characterization reports produced and delivered by the U.S. Geological Survey Earth Resources Observation and Science Cal/Val Center of Excellence. These reports present and detail the methodology and procedures for characterization; present technical and operational information about the EnMAP hyperspectral sensor; and provide a summary of test measurements, data retention practices, data analysis results, and conclusions. The Earth Resources Observation and Science Cal/Val Center of Excellence system characterization team completed data analyses to characterize the geometric (interior and exterior), and radiometric performances of the EnMAP hyperspectral sensor. Results of these analyses indicate that the Environmental Mapping and Analysis Program has a band-to-band geometric performance in the range of −0.135 to 0.15 pixel, geometric performance relative to the Operational Land Imager in the range of −27.716 meters (−0.92 pixel) to 32.892 meters (1.09 pixels) offset in comparison to Landsat 8 Operational Land Imager, offset of a radiometric comparison in the range of −0.012 to 0.020, slope of a radiometric comparison in the range of 0.947 to 1.031.

Open-File Report

Potentially toxic elements in wild Agassiz’s desert tortoises: Tissue concentrations and association with disease

Background: Desert tortoise ( Gopherus agassizii ) populations have continued to decline due to infectious and other diseases, predation, and habitat alteration. The potential contribution of minerals and heavy metals to tortoise health and susceptibility to disease remains uncertain. Objective: The objective of this study was to evaluate the results of elemental analysis of trace minerals and macrominerals in scute keratin, kidney, and liver tissue from ill and dying desert tortoises salvaged for necropsy between 1993 and 2000. Methods: Salvaged tortoises were categorized by age (adult, juvenile), geographic location, and primary disease based on necropsy findings. A subset of tortoises that were injured or killed by vehicular trauma or predation but with no notable pathologic abnormalities were used as controls. A panel of 21 trace minerals and 6 macrominerals was analyzed in scute keratin, kidney, and liver tissue samples by inductively-coupled plasma spectrometry and atomic absorption spectrophotometry. Results: Necropsies were done on 46 tortoises, including 9 juveniles salvaged from 5 regions in the Colorado and Mojave deserts of California. Primary diseases were cutaneous dyskeratosis (n=9), infection/ inflammation (n=8), malnutrition (n=7), mycoplasmosis (n=5), and urolithiasis (n=3); 14 tortoises were classified as controls. Concentrations of elements differed significantly by tissue, age, desert region, and disease (P < 0.05). Tortoises with cutaneous dyskeratosis had significantly higher Se concentrations, primarily in keratin and liver, than tortoises with other diseases (P < 0.001). Juveniles were more likely than adults to have high Pb, Sn, and Zn levels (P < 0.05). All tortoises had detectable levels of more than one potentially toxic heavy metal, including As, Cd, Cr, Hg, Ni, Pb, Sn and V. Conclusions: Potentially toxic elements are frequently found in tissue from tortoises in desert regions of California, with significantly higher concentrations in diseased tortoises. Metal exposure from soils, mining, historic and ongoing military activities, and other human activities could increase susceptibility to disease in desert tortoises.

California

Divisions of geologic time—Major chronostratigraphic and geochronologic units

Introduction The reports and maps of our Nation’s geological surveys inform and benefit the public, private industry, government officials, and scientists. The use of clear and consistent nomenclature and classifications can improve communication of data and interpretations. Since 1899, the U.S. Geological Survey (USGS) Geologic Names Committee (GNC) has been responsible for defining standards that promote uniform geologic nomenclature and classifications among geoscientists. The GNC periodically publishes a geologic time scale, the “Divisions of Geologic Time,” that serves as the national standard for USGS publications (for example, refer to Orndorff and others, 2023). Authors may use other published geologic time scales, such as those of the Geological Society of America (GSA) or the International Commission on Stratigraphy (ICS), provided that they are clearly specified and referenced. Access to the USGS, GSA, and ICS geologic time scales is also available from the U.S. Geologic Names Lexicon (Geolex) website https://ngmdb.usgs.gov/Geolex/stratres/timescales. The geologic time scale serves a dual purpose by enabling authors to distinguish earth material units by position (chronostratigraphic) and time (geochronologic), as outlined in order of decreasing rank.

Fact Sheet

Integrating Sr isotopes, microchemistry, and genetics to reconstruct Salmonidae species and life history

Recent approaches to fisheries research emphasize the importance of the coproduction of knowledge in building resilient and culturally mindful fisheries management frameworks. Despite widespread recognition of the need for Indigenous knowledge and historical reference points as baseline data, archaeological data are rarely included in conservation biology research designs. Here we propose a novel multiproxy method to learn from former fisheries stewards by generating archaeological data on past salmonid population parameters. We used a newly developed, high throughput qPCR (HT-qPCR) chip, originally designed for environmental DNA (eDNA), for species identification of archaeological salmonid vertebrae. We combine this with the laser ablation split-stream (LASS) approach to identify ocean-migration versus freshwater residency. We test this multidisciplinary approach using both contemporary and archaeological salmonid samples and new radiocarbon dates from the Tronsdal Site on the Skagit River, Washington State, USA. This is a useful approach for extracting information about Salmonidae species and life history diversity from archaeological remains to reconstruct historic baselines for several population parameters in anadromous species with long periods of freshwater residency. The approach outlined in this paper may be particularly useful for research investigating past fisheries dynamics, offering hundreds to thousands of years of temporal depth for modern fisheries management, harvest policies, restoration ecology, and conservation biology.

Idaho, Oregon, Washington

Surficial geologic map of the Owlshead Mountains 30' x 60' quadrangle, Inyo and San Bernardino Counties, California

The surficial geologic map of the Owlshead Mountains 30' x 60' quadrangle depicts the distribution and characteristics of surficial-deposit materials and neotectonic deformation for an area of approximately 5,000 square kilometers (km 2 ) located in the western Basin and Range Province of eastern California. The map represents a new compilation of the surficial geology that encompasses deposits within the late Pliocene to Quaternary. The map is based primarily on new mapping conducted between 2001 and 2009. Map compilation was supported by field observations distributed across the map area, combined with reference to several published and unpublished mapping sources that mostly emphasized neotectonic deformation. The surficial-deposit units included in the map follow a classification scheme that systematically denotes depositional process, relative age, and any secondary sedimentologic or morphologic characteristics. Identification, correlation, and age estimation of map units are based primarily on the relative degree of development of certain time-dependent characteristics such as surface morphology, including local dissection and surface preservation, surface clast modification, and degree of soil development; these characteristics are implicitly incorporated into unit designations. The map represents a detailed and regionally uniform synthesis of the late Neogene geology for this large area that provides a framework applicable to many interpretative studies, such as regional patterns of deposition and dissection; surface drainage development and evolution; and the distribution, style, and timing of neotectonic deformation.

California

Hydrological control on soil redox condition and carbon loss of coastal wetland under sea-kevel rise

Coastal wetlands are critical carbon sinks with their biogeochemical and ecological functioning shaped by dynamic hydrological conditions that are increasingly influenced by climate change. A key unresolved question is how hydrologic flow, vegetation response, and rising sea levels interact to regulate soil redox condition and carbon loss in coastal wetlands. Using a field-tested hydrological–biogeochemical–ecological modeling framework, we reveal how the interplay between terrestrial groundwater discharge and tidal fluctuations generates complex groundwater flow patterns at the terrestrial–aquatic interface, and how these patterns modulate soil redox conditions, in turn influencing soil organic matter decomposition and carbon loss. Notably, rising sea levels suppress soil CO 2 emissions while reducing lateral dissolved carbon losses, thereby enhancing litter carbon sequestration under anoxic conditions. As vegetation responds to sea-level rise and carbon inputs diminish, litter carbon subsequently declines. These findings underscore a critical hydrological control on carbon cycling, advancing our understanding of coastal ecosystem resilience in a warming world.

Louisiana

Mapping eelgrass (Zostera marina) cover and biomass at Izembek Lagoon, Alaska, using in-situ field data and Sentinel-2 satellite imagery

The U.S. Geological Survey and the U.S. Fish and Wildlife Service have developed a three-tiered strategy for monitoring eelgrass ( Zostera marina ) beds at Izembek Lagoon, Alaska, that targets different spatial and temporal scales. The broadest-scale monitoring (tier-1) uses satellite imagery about every 5 years to delineate the spatial extent of eelgrass beds throughout the lagoon. This report describes the most recent (mid-2020s) tier-1 eelgrass monitoring at Izembek Lagoon. The monitoring effort began by canvasing all satellite imagery collected during summer, under clear daytime skies and at low-tide, since the last tier-1 effort in 2006. Two eelgrass maps of Izembek Lagoon were generated by first creating maps of spectrally unique classes from two Sentinel-2 satellite images collected on July 1, 2016, and August 14, 2020, then attributing those spectral classes with information about eelgrass conditions based on field data. Specifically, maps depicting various eelgrass metrics, such as percentage of cover and modeled biomass, were generated using summaries of the ground data that spatially intersected each spectral class. Comparisons of the 2016 and 2020 Sentinel-2 maps showing eelgrass distributional extent, as well as a 2006 Landsat map, indicated that areas where eelgrass presence may have declined during 2006–20 were most prevalent in the central part of Izembek Lagoon. More recently, during 2016-20, areas of possible biomass decline were more prevalent in the southern part of the lagoon. Monitoring eelgrass conditions at Izembek Lagoon with satellite imagery and concurrent ground data allows conditions to be compared over time, but the influences of tide levels, growing season phenology, and spatiotemporal co-registration accuracy should be considered when designing and interpreting change detection analyses.

Alaska

Sediment thickness map of United States Atlantic and Gulf Coastal Plain Strata, and their influence on earthquake ground motions

With the recent successful accounting of basin depth ground-motion adjustments in seismic hazard analyses for select areas of the western United States, we move toward implementing similar adjustments in the Atlantic and Gulf Coastal Plains by constructing a sediment thickness model and evaluating multiple relevant site amplification models for central and eastern United States seismic hazard analyses. We digitize and combine existing sediment thickness data sets into a composite surface that delineates the base of Cretaceous sediments under the Atlantic Coastal Plain and the base of Mesozoic sediments under the Gulf Coastal Plain. Amplification models dependent on sediment thickness, site natural period, and source-to-site path length are compared with data sets of observed ground motions to evaluate the ability of the new models to improve ground motion estimates. We find that the amplification models can account for observed trends in sediment-thickness and period-dependent residuals, but some tuning is required. For example, the model of Chapman and Guo requires a reference V S 30 , the time-averaged shear-wave velocity within 30 m of the Earth’s surface, for non-Coastal Plain sites, which we estimate to be between about 1 and 2 km/s. Along with our sediment thickness model, we estimate a velocity profile for application to the Harmon et al. site-natural-period-based model in order to best match the Chapman and Guo period dependence for a broad range of sediment thicknesses. The Next Generation of Attenuation models for the eastern United States Gulf Coast path-based adjustment models can also account for seismic attenuation in the Coastal Plain sediments and reduce the standard deviation of total residuals. If enacted in the U.S. Geological Survey National Seismic Hazard Model, these amplification models will reduce predicted short-period (<1 s) and increase predicted long-period (>1 s) ground motions in the Coastal Plains appreciably.

Earthquake Spectra

Deep-ocean macrofaunal assemblages on ferromanganese and phosphorite-rich substrates in the Southern California Borderland

Mineral-rich hardgrounds, such as ferromanganese (FeMn) crusts and phosphorites, occur on seamounts and continental margins, gaining attention for their resource potential due to their enrichment in valuable metals in some regions. This study focuses on the Southern California Borderland (SCB), an area characterized by uneven and heterogeneous topography featuring FeMn crusts, phosphorites, basalt, and sedimentary rocks that occur at varying depths and are exposed to a range of oxygen concentrations. Due to its heterogeneity, this region serves as an optimal setting for investigating the relationship between mineral-rich hardgrounds and benthic fauna. This study characterizes the density, diversity, and community composition of macrofauna (>300 μm) on hardgrounds as a function of substrate type and environment (depth and oxygen ranges). Rocks and their macrofauna were sampled quantitatively using remotely operated vehicles (ROVs) during expeditions in 2020 and 2021 at depths above, within, and below the oxygen minimum zone (OMZ). A total of 3,555 macrofauna individuals were counted and 416 different morphospecies (excluding encrusting bryozoans and hydrozoans) were identified from 82 rocks at depths between 231 and 2,688 m. Average density for SCB macrofauna was 11.08 ± 0.87 ind. 200 cm −2 and mean Shannon-Wiener diversity per rock (H′ [loge] ) was 2.22 ± 0.07. A relationship was found between substrate type and macrofaunal communities. Phosphorite rocks had the highest H′ of the four substrates compared on a per-rock basis. However, when samples were pooled by substrate, FeMn crusts had the highest H′ and rarefaction diversity. Of all the environmental variables examined, water depth explained the largest variance in macrofaunal community composition. Macrofaunal density and diversity values were similar at sites within and outside the OMZ. This study is the first to analyze the macrofaunal communities of mineral-rich hardgrounds in the SCB, which support deep-ocean biodiversity by acting as specialized substrates for macrofaunal communities. Understanding the intricate relationships between macrofaunal assemblages and mineral-rich substrates may inform effects from environmental disruptions associated with deep-seabed mining or climate change. The findings contribute baseline information useful for effective conservation and management of the SCB and will support scientists in monitoring changes in these communities due to environmental disturbance or human impact in the future.

California