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At least 487 records · Page 27Linked to original sources

USGS2011

Description of an elasmobranch TCR coreceptor: CD8α from Rhinobatos productus

Cell-mediated immunity plays an essential role for the control and eradication of intracellular pathogens. To learn more about the evolutionary origins of the first signal (Signal 1) for T-cell activation, we cloned CD8α from an elasmobranch, Rhinobatos productus. Similar to full-length CD8α cDNAs from other vertebrates, Rhpr-CD8α (1800 bp) encodes a 219 amino acid open reading frame composed of a signal peptide, an extracellular IgSF V domain and a stalk/hinge region followed by a well-conserved transmembrane domain and cytoplasmic tail. Overall, the mature Rhpr-CD8α protein (201 aa) displays ~30% amino acid identity with mammalian CD8α including absolute conservation of cysteine residues involved in the IgSf V domain fold and dimerization of CD8αα and CD8αβ. One prominent feature is the absence of the LCK association motif (CXC) that is needed for achieving signal 1 in tetrapods. Both elasmobranch and teleost CD8α protein sequences possess a similar but distinctly different motif (CXH) in the cytoplasmic tail. The overall genomic structure of CD8α has been conserved during the course of vertebrate evolution both for the number of exons and phase of splicing. Finally, quantitative RTPCR demonstrated that elasmobranch CD8α is expressed in lymphoid-rich tissues similar to CD8 in other vertebrates. The results from this study indicate the existence of CD8 prior to the emergence of the gnathostomes (>450 MYA) while providing evidence that the canonical LCK association motif in mammals is likely a derived characteristic of tetrapod CD8α, suggesting potential differences for T-cell education and activation in the various gnathostomes.

Developmental and Comparative Immunology↗

USGS2010

Hydraulic modeling of mussel habitat at a bridge-replacement site, Allegheny River, Pennsylvania, USA

The Allegheny River in Pennsylvania supports a large and diverse freshwater-mussel community, including two federally listed endangered species, Pleurobema clava (Clubshell) and Epioblasma torulosa rangiana (Northern Riffleshell). It is recognized that river hydraulics and morphology play important roles in mussel distribution. To assess the hydraulic influences of bridge replacement on mussel habitat, metrics such as depth, velocity, and their derivatives (shear stress, Froude number) were collected or computed. The objectives of the project were to evaluate mussel and hydraulic data at a reference site and to compare those findings to a bridge-replacement site. The findings were used to support a statistical analysis, which establishes correlations between mussel count and hydraulics, and a numerical model to forecast habitat based on the statistics. ArcGIS was selected to manage the data and generate a grid to compute area statistics for 3319, 4.9-m × 4.9-m cells (cell) for total mussel count, depth, velocity, shear stress, and Froude number. The Wilcoxon Rank Sum test indicated no statistical significance between the total mussel count and the hydraulic variables; however, trellis graphs were used to account for the spatial variability in the data set. For the flow conditions measured, the total mussel count per cell is greatest at sections where (1) velocities range from 0.061 to 0.21 m/s, (2) shear stresses range from 0.48 to 3.8 dyne/cm 2 , and (3) Froude numbers range from 0.006 to 0.04. Based on the statistical targets established, the hydraulic model results suggest that an additional 2428 m 2 or a 30-percent increase in suitable mussel habitat could be generated at the replacement-bridge site when compared to the baseline condition associated with the existing bridge at that same location. The study did not address the influences of substrate, acid mine drainage, sediment loads from tributaries, and surface-water/ground-water exchange on mussel habitat. Future studies could include methods for quantifying (1) channel–substrate composition and distribution using tools such as hydroacoustic echosounders specifically designed and calibrated to identify bed composition and mussel populations, (2) surface-water and ground-water interactions, and (3) a high-streamflow event.

Pennsylvania↗

USGS2012

Plant toxins and trophic cascades alter fire regime and succession on a boral forest landscape

Two models were integrated in order to study the effect of plant toxicity and a trophic cascade on forest succession and fire patterns across a boreal landscape in central Alaska. One of the models, ALFRESCO, is a cellular automata model that stochastically simulates transitions from spruce dominated 1 km2 spatial cells to deciduous woody vegetation based on stochastic fires, and from deciduous woody vegetation to spruce based on age of the cell with some stochastic variation. The other model, the ‘toxin-dependent functional response’ model (TDFRM) simulates woody vegetation types with different levels of toxicity, an herbivore browser (moose) that can forage selectively on these types, and a carnivore (wolf) that preys on the herbivore. Here we replace the simple succession rules in each ALFRESCO cell by plant–herbivore–carnivore dynamics from TDFRM. The central hypothesis tested in the integrated model is that the herbivore, by feeding selectively on low-toxicity deciduous woody vegetation, speeds succession towards high-toxicity evergreens, like spruce. Wolves, by keeping moose populations down, can help slow the succession. Our results confirmed this hypothesis for the model calibrated to the Tanana floodplain of Alaska. We used the model to estimate the effects of different levels of wolf control. Simulations indicated that management reductions in wolf densities could reduce the mean time to transition from deciduous to spruce by more than 15 years, thereby increasing landscape flammability. The integrated model can be useful in estimating ecosystem impacts of wolf control and moose harvesting in central Alaska.

Alaska↗

USGS2024

In vitro impacts of glyphosate on manatee lymphocytes

Exposure to contaminants, such as the herbicide glyphosate, can suppress protective immune functions. Glyphosate is the herbicide most used worldwide and has been found in the plasma of more than 50 % of the Florida manatees ( Trichechus manatus latirostris ) and all-year-round in their aquatic environment. Our objectives were to analyze the consequences of glyphosate exposure on their immune responses via T-lymphocyte proliferation assays and transcriptomics. We isolated peripheral blood mononuclear cells (mainly lymphocytes) of free-ranging manatees and performed T-cell proliferation assays. We used transcriptomics to understand the consequences of glyphosate in vitro exposure. The three doses chosen ranged from environmentally relevant concentrations at 10 to 10,000 µg.L −1 that is considered an environmental contamination scenario. Glyphosate caused a dose-dependent reduction in T-lymphocyte proliferation, with a significant mean reduction of 27.3 % at 10,000 µg.L −1 and up to 51.5 % in some individuals. Additionally, T-lymphocyte proliferation was significantly reduced in mid-winter compared to early winter. Transcriptomic analysis of peripheral blood mononuclear cells indicated that all doses of glyphosate (10, 1,000, and 10,000 µg.L −1 ) resulted in up-regulation of genes related to acute phase inflammation and inhibition of the T-lymphocyte proliferation pathway. Exposure to this contaminant along with other environmental stressors, such as extreme winters and red tide, might further affect the adaptive immune response of this threatened species.

Florida↗

USGS2010

Transcriptome analysis of rainbow trout infected with high and low virulence strains of Infectious hematopoietic necrosis virus

There are three main genetic lineages or genogroups of Infectious hematopoietic necrosis virus (IHNV) in N. America. Strains representing the M genogroup are more virulent in rainbow trout relative to the U genogroup. In this study, we used microarray analysis to evaluate potential mechanisms responsible for host-specific virulence in rainbow trout that were given intraperitoneal injections of buffer or a representative M or U type virus strain. Reverse transcriptase quantitative PCR (RT-qPCR) was used to assess viral load and gene expression of select immune genes. Viral load was significantly higher in trout infected with the M virus starting at 24 h post-infection (p.i.) and continuing until 72 h p.i. Microarray analysis of the 48 h time point revealed 153 up-regulated and 248 down-regulated features in response to M virus infection but only 62 up-regulated and 49 down-regulated features following U virus infection. Translation and transcription features were among the most frequent down-regulated features in response to M virus infection and may be associated with the host cell shutoff phenomenon. A greater host cell shutoff response by the M virus may facilitate subversion of the host cell transcriptional machinery and enhance viral replication, suggesting the M virus may be better optimized to manipulate the rainbow trout transcriptional and translational machinery. Anti-viral associated features were the most commonly up-regulated features. A common set of features were up-regulated in both the M and U infection groups, but were induced to a higher magnitude in the M infection group. Gene expression of the anti-viral genes Mx-1 and Vig-1 was correlated but not entirely dependent on viral load in the anterior kidney. Slower replication of the U virus may allow the host more time to induce protective anti-viral immune mechanisms.

Fish and Shellfish Immunology↗

USGS2007

A unified equation for calculating methane vapor pressures in the CH4-H2O system with measured Raman shifts

A unified equation has been derived by using all available data for calculating methane vapor pressures with measured Raman shifts of C-H symmetric stretching band (??1) in the vapor phase of sample fluids near room temperature. This equation eliminates discrepancies among the existing data sets and can be applied at any Raman laboratory. Raman shifts of C-H symmetric stretching band of methane in the vapor phase of CH4-H2O mixtures prepared in a high-pressure optical cell were also measured at temperatures between room temperature and 200 ??C, and pressures up to 37 MPa. The results show that the CH4 ??1 band position shifts to higher wavenumber as temperature increases. We also demonstrated that this Raman band shift is a simple function of methane vapor density, and, therefore, when combined with equation of state of methane, methane vapor pressures in the sample fluids at elevated temperatures can be calculated from measured Raman peak positions. This method can be applied to determine the pressure of CH4-bearing systems, such as methane-rich fluid inclusions from sedimentary basins or experimental fluids in hydrothermal diamond-anvil cell or other types of optical cell. ?? 2007 Elsevier Ltd. All rights reserved.

Geochimica et Cosmochimica Acta↗

USGS2018

Growth-suppressing and algicidal properties of an extract from Arundo donax, an invasive riparian plant, against Prymnesium parvum, an invasive harmful alga

This study examined the ability of acidic and neutral/alkaline fractions of a methanolic extract from giant reed ( Arundo donax ) and of two of its constituents, gramine and skatole, to inhibit growth of the ichthyotoxic golden alga (Prymnesium parvum ) in batch culture. For this study, growth suppression was defined as inhibition of maximum cell density, algicidal activity as early occurrence of negative growth, and algistatic activity as lack of net growth. The acidic fraction did not affect algal growth. The neutral/alkaline fraction showed growth-suppressing and algicidal activities but no signs of algistatic activity – namely, cells in cultures surviving a partial-algicidal exposure concentration (causing transient negative growth) were later able to initiate positive growth but at higher concentrations, algicidal activity was full and irreversible. Gramine suppressed growth more effectively than skatole and at the highest concentration tested, gramine also showed partial-algicidal and algistatic activity. While the partial-algicidal activities of the neutral/alkaline fraction and of gramine were short-lived (≤6 days) and thus may share similar mechanisms, algistatic activity was unique to gramine and persisted for >3 weeks. Given gramine’s reported concentration in the neutral/alkaline fraction, its corresponding level of algicidal activity is much lower than the fraction’s suggesting the latter contains additional potent algicides. Inhibition of maximum cell density by all test compounds was associated with reductions in exponential growth rate, and in the case of the neutral/alkaline fraction and gramine also reductions in early (pre-exponential) growth. These results indicate that giant reed is a potential source of natural products to control golden alga blooms. Giant reed is an invasive species in North America, thus also providing incentive for research into strategies to couple management efforts for both species.

Harmful Algae↗

USGS2014

Analysis of projected water availability with current basin management plan, Pajaro Valley, California

The projection and analysis of the Pajaro Valley Hydrologic Model (PVHM) 34 years into the future using MODFLOW with the Farm Process (MF-FMP) facilitates assessment of potential future water availability. The projection is facilitated by the integrated hydrologic model, MF-FMP that fully couples the simulation of the use and movement of water from precipitation, streamflow, runoff, groundwater flow, and consumption by natural and agricultural vegetation throughout the hydrologic system at all times. MF-FMP allows for more complete analysis of conjunctive-use water-resource systems than previously possible with MODFLOW by combining relevant aspects of the landscape with the groundwater and surface-water components. This analysis is accomplished using distributed cell-by-cell supply-constrained and demand-driven components across the landscape within “water-balance subregions” (WBS) comprised of one or more model cells that can represent a single farm, a group of farms, watersheds, or other hydrologic or geopolitical entities. Analysis of conjunctive use would be difficult without embedding the fully coupled supply-and-demand into a fully coupled simulation, and are difficult to estimate a priori. The analysis of projected supply and demand for the Pajaro Valley indicate that the current water supply facilities constructed to provide alternative local sources of supplemental water to replace coastal groundwater pumpage, but may not completely eliminate additional overdraft. The simulation of the coastal distribution system (CDS) replicates: 20 miles of conveyance pipeline, managed aquifer recharge and recovery (MARR) system that captures local runoff, and recycled-water treatment facility (RWF) from urban wastewater, along with the use of other blend water supplies, provide partial relief and substitution for coastal pumpage (aka in-lieu recharge). The effects of these Basin Management Plan (BMP) projects were analyzed subject to historical climate variations and assumptions of 2009 urban water demand and land use. Water supplied directly from precipitation, and indirectly from reuse, captured local runoff, and groundwater is necessary but inadequate to satisfy agricultural demand without coastal and regional storage depletion that facilitates seawater intrusion. These facilities reduce potential seawater intrusion by about 45% with groundwater levels in the four regions served by the CDS projected to recover to levels a few feet above sea level. The projected recoveries are not high enough to prevent additional seawater intrusion during dry-year periods or in the deeper aquifers where pumpage is greater. While these facilities could reduce coastal pumpage by about 55% of the historical 2000–2009 pumpage for these regions, and some of the water is delivered in excess of demand, other coastal regions continue to create demands on coastal pumpage that will need to be replaced to reduce seawater intrusion. In addition, inland urban and agricultural demands continue to sustain water levels below sea level causing regional landward gradients that also drive seawater intrusion. Seawater intrusion is reduced by about 45% but it supplies about 55% of the recovery of groundwater levels in the coastal regions served by the CDS. If economically feasible, water from summer agricultural runoff and tile-drain returnflows could be another potential local source of water that, if captured and reused, could offset the imbalance between supply and demand as well as reducing discharge of agricultural runoff into the National Marine Sanctuary of Monterey Bay. A BMP update (2012) identifies projects and programs that will fund a conservation program and will provide additional, alternative water sources to reduce or replace coastal and inland pumpage, and to replenish the aquifers with managed aquifer recharge in an inland portion of the Pajaro Valley.

California↗

USGS2009

Assessment of the UV camera sulfur dioxide retrieval for point source plumes

Digital cameras, sensitive to specific regions of the ultra-violet (UV) spectrum, have been employed for quantifying sulfur dioxide (SO 2 ) emissions in recent years. The instruments make use of the selective absorption of UV light by SO 2 molecules to determine pathlength concentration. Many monitoring advantages are gained by using this technique, but the accuracy and limitations have not been thoroughly investigated. The effect of some user-controlled parameters, including image exposure duration, the diameter of the lens aperture, the frequency of calibration cell imaging, and the use of the single or paired bandpass filters, have not yet been addressed. In order to clarify methodological consequences and quantify accuracy, laboratory and field experiments were conducted. Images were collected of calibration cells under varying observational conditions, and our conclusions provide guidance for enhanced image collection. Results indicate that the calibration cell response is reliably linear below 1500 ppm m, but that the response is significantly affected by changing light conditions. Exposure durations that produced maximum image digital numbers above 32 500 counts can reduce noise in plume images. Sulfur dioxide retrieval results from a coal-fired power plant plume were compared to direct sampling measurements and the results indicate that the accuracy of the UV camera retrieval method is within the range of current spectrometric methods. ?? 2009 Elsevier B.V.

Journal of Volcanology and Geothermal Research↗

USGS2020

Spatial variability of phytoplankton in a shallow tidal freshwater system reveals complex controls on abundance and community structure

Estuaries worldwide are undergoing changes to patterns of aquatic productivity because of human activities that alter flow, impact sediment delivery and thus the light field, and contribute nutrients and contaminants like pesticides and metals. These changes can influence phytoplankton communities, which in turn can alter estuarine food webs. We used multiple approaches-including high-resolution water quality mapping, synoptic sampling, productivity and nitrogen uptake rates, Lagrangian parcel tracking, enclosure experiments and bottle incubations-over a short time period to take a “spatial snapshot” of conditions in the northern region of the San Francisco Estuary (California, USA) to examine how environmental drivers like light availability, nutrients, water residence time, and contaminants affect phytoplankton abundance and community attributes like size distribution, taxonomic structure, and nutrient uptake rates. Zones characterized by longer residence time (15–60 days) had higher chlorophyll-a concentrations (9 ± 4 µg L −1 ) and were comprised primarily of small phytoplankton cells (<5 µm, 74 ± 8%), lower ammonium concentrations (1 ± 0.8 µM), higher nitrate uptake rates, and higher rates of potential carbon productivity. Conversely, zones characterized by shorter residence time (1–14 days) had higher ammonium concentration (13 ± 5 µM) and lower chlorophyll-a concentration (5 ± 1 µg L −1 ) with diatoms making up a larger percent contribution. Longer residence time, however, did not result in the accumulation of large (>5 µm) cells considered important to pelagic food webs. Rather, longer residence time zones had a phytoplankton community comprised primarily of small cells, particularly picocyanobacteria that made up 38 ± 17% of the chlorophyll-a – nearly double the concentration seen in shorter residence time zones (22 ± 7% picocyanobacterial of chlorophyll-a). Our results suggest that water residence time in estuaries may have an effect as large or larger than that experimentally demonstrated for light, contaminants, or nutrients.

California↗

USGS2015

Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch ( Perca flavescens )

Declining harvests of yellow perch, Perca flavescens , in urbanized watersheds of Chesapeake Bay have prompted investigations of their reproductive fitness. The purpose of this study was to establish a flow cytometric technique for DNA analysis of fixed samples sent from the field to provide reliable gamete quality measurements. Similar to the sperm chromatin structure assay, measures were made on the susceptibility of nuclear DNA to acid-induced denaturation, but used fixed rather than live or thawed cells. Nuclei were best exposed to the acid treatment for 1 minute at 37 °C followed by the addition of cold (4 °C) propidium iodide staining solution before flow cytometry. The rationale for protocol development is presented graphically through cytograms. Field results collected in 2008 and 2009 revealed DNA fragmentation up to 14.5%. In 2008, DNA fragmentation from the more urbanized watersheds was significantly greater than from reference sites (P = 0.026) and in 2009, higher percentages of haploid testicular cells were noted from the less urbanized watersheds (P = 0.032) indicating better reproductive condition at sites with less urbanization. For both years, total and progressive live sperm motilities by computer-assisted sperm motion analysis ranged from 19.1% to 76.5%, being significantly higher at the less urbanized sites (P < 0.05). This flow cytometric method takes advantage of the propensity of fragmented DNA to be denatured under standard conditions, or 1 minute at 37 °C with 10% buffered formalin–fixed cells. The study of fixed sperm makes possible the restrospective investigation of germplasm fragmentation, spermatogenic ploidy patterns, and chromatin compaction levels from samples translocated over distance and time. The protocol provides an approach that can be modified for other species across taxa.

Theriogenology↗

USGS2023

SARS-CoV-2 utilization of ACE2 from different bat species allows for virus entry and replication in vitro

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is believed to have a zoonotic origin with bats suspected as a natural host. In this work, we individually express the ACE2 of seven bat species including, little brown, great roundleaf, Pearson's horseshoe, greater horseshoe, Brazilian free-tailed, Egyptian rousette, and Chinese rufous horseshoe in DF1 cells and determine their ability to support attachment and replication of SARS-CoV-2 viruses. We demonstrate that the ACE2 receptor of all seven species made DF1 cells permissible to SARS-CoV-2. The level of virus replication differed between bat species and variants tested. The Wuhan lineage SARS-CoV-2 virus replicated to higher titers than either variant virus tested. All viruses tested grew to higher titers in cells expressing the human ACE2 gene compared to a bat ACE2. This study provides a practical in vitro method for further testing of animal species for potential susceptibility to current and emerging SARS-CoV-2 viruses.

Virology↗

USGS2017

Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout ( Oncorhynchus mykiss )

A novel virus, rainbow trout orthomyxovirus (RbtOV), was isolated in 1997 and again in 2000 from commercially-reared rainbow trout ( Oncorhynchus mykiss ) in Idaho, USA. The virus grew optimally in the CHSE-214 cell line at 15°C producing a diffuse cytopathic effect; however, juvenile rainbow trout exposed to cell culture-grown virus showed no mortality or gross pathology. Electron microscopy of preparations from infected cell cultures revealed the presence of typical orthomyxovirus particles. The complete genome of RbtOV is comprised of eight linear segments of single-stranded, negative-sense RNA having highly conserved 5′ and 3′-terminal nucleotide sequences. Another virus isolated in 2014 from steelhead trout (also O. mykiss ) in Wisconsin, USA, and designated SttOV was found to have eight genome segments with high amino acid sequence identities (89–99%) to the corresponding genes of RbtOV, suggesting these new viruses are isolates of the same virus species and may be more widespread than currently realized. The new isolates had the same genome segment order and the closest pairwise amino acid sequence identities of 16–42% with Infectious salmon anemia virus (ISAV), the type species and currently only member of the genus Isavirus in the family Orthomyxoviridae . However, pairwise comparisons of the predicted amino acid sequences of the 10 RbtOV and SttOV proteins with orthologs from representatives of the established orthomyxoviral genera and a phylogenetic analysis using the PB1 protein showed that while RbtOV and SttOV clustered most closely with ISAV, they diverged sufficiently to merit consideration as representatives of a novel genus. A set of PCR primers was designed using conserved regions of the PB1 gene to produce amplicons that may be sequenced for identification of similar fish orthomyxoviruses in the future.

Virus Research↗

USGS2025

Comparative genomics of Bacillus anthracis A and B-clades reveals genetic variation in genes responsible for spore germination

Bacillus anthracis , the causative agent of anthrax, is composed of three genetic clades (A, B, and C). Clade-A is the most common and distributed worldwide, B-clade has a narrow geographic distribution, and C-clade is rare. South Africa's Kruger National Park (KNP) has high diversity of B. anthracis , with strains from A and B clades described from its northernmost region, Pafuri. We employed whole genome sequencing to investigate the genomic diversity of B. anthracis strains isolated from animal carcasses ( n = 34) during the 2012–2015 outbreaks. Whole-genome single-nucleotide polymorphism (wgSNP) analysis assigned the 2012–2015 B. anthracis genomes to the A-clade branch, distributed across the branch's two minor sub-clades A.Br.005/006. Additionally, pan-genomic analysis distinguished the A- and B-clade genomes, identifying unique accessory genes. Notable genetic differences include the biosynthetic spore cell wall genes; long-chain fatty acid CoA ligases ( FaD13 ), Bacillus collagen-like protein of anthracis (BclA) involved in the exosporium germination, as well as a truncated murein DD-endopeptidase ( mepH ) found in the pXO2 plasmid of the B-clade strains. The tryptophan synthase subunit alpha gene ( trpA ), which results in a pseudogene in B-clade genomes separates the A- and B-clade genomes. These differences in biosynthetic cell wall genes suggest variation in adaptability or cell growth of the B-clade strains in the environment, further influenced by the truncation of the trpA gene involved in spore germination. The A.Br.005/006-clade strains in KNP exhibit higher genetic diversity, which may enhance their resilience to environmental stressors. In contrast, the KNP B-clade (B.Br.001/002) strains show limited genetic variation, potentially reducing their adaptability. This pattern is evident through whole-genome SNP analysis and pan-genomics investigating the evolution of B. anthracis .

Kruger National Park↗

USGS2003

Pore space analysis of NAPL distribution in sand-clay media

This paper introduces a conceptual model of clays and non-aqueous phase liquids (NAPLs) at the pore scale that has been developed from a mathematical unit cell model, and direct micromodel observation and measurement of clay-containing porous media. The mathematical model uses a unit cell concept with uniform spherical grains for simulating the sand in the sand-clay matrix (???10% clay). Micromodels made with glass slides and including different clay-containing porous media were used to investigate the two clays (kaolinite and montmorillonite) and NAPL distribution within the pore space. The results were used to understand the distribution of NAPL advancing into initially saturated sand and sand-clay media, and provided a detailed analysis of the pore-scale geometry, pore size distribution, NAPL entry pressures, and the effect of clay on this geometry. Interesting NAPL saturation profiles were observed as a result of the complexity of the pore space geometry with the different packing angles and the presence of clays. The unit cell approach has applications for enhancing the mechanistic understanding and conceptualization, both visually and mathematically, of pore-scale processes such as NAPL and clay distribution. ?? 2003 Elsevier Science Ltd. All rights reserved.

Advances in Water Resources↗

USGS2016

Amino acid specific stable nitrogen isotope values in avian tissues: Insights from captive American kestrels and wild herring gulls

Through laboratory and field studies, the utility of amino acid compound-specific nitrogen isotope analysis (AA-CSIA) in avian studies is investigated. Captive American kestrels ( Falco sparverius ) were fed an isotopically characterized diet and patterns in δ 15 N values of amino acids (AAs) were compared to those in their tissues (muscle and red blood cells) and food. Based upon nitrogen isotope discrimination between diet and kestrel tissues, AAs could mostly be categorized as source AAs (retaining baseline δ 15 N values) and trophic AAs (showing 15 N enrichment). Trophic discrimination factors based upon the source (phenylalanine, Phe) and trophic (glutamic acid, Glu) AAs were 4.1 (muscle) and 5.4 (red blood cells), lower than those reported for metazoan invertebrates. In a field study involving omnivorous herring gulls ( Larus argentatus smithsonianus ), egg AA isotopic patterns largely retained those observed in the laying female’s tissues (muscle, red blood cells, and liver). Realistic estimates of gull trophic position were obtained using bird Glu and Phe δ 15 N values combined with β values (difference in Glu and Phe δ 15 N in primary producers) for aquatic and terrestrial food webs. Egg fatty acids were used to weight β values for proportions of aquatic and terrestrial food in gull diets. This novel approach can be applied to generalist species that feed across ecosystem boundaries.

Environmental Science & Technology↗

USGS2023

Establishing an in vitro model to assess the toxicity of 6PPD-quinone and other tire wear transformation products

The tire wear transformation product 6PPD-quinone (6PPDQ) has been implicated as the causative factor for broad scale mortality events for coho salmon in the Pacific Northwest. Highly variable sensitivity to 6PPDQ in closely related salmonids complicates efforts to evaluate the broader toxicological impacts to aquatic ecosystems. Our goals were to (1) validate the large range of in vivo species sensitivities reported for coho, Chinook, and sockeye salmon and (2) develop an in vitro platform for assessing 6PPDQ toxicity. In vivo studies confirmed the acute sensitivity of juvenile coho (12 h LC 50 = 80.4 ng/L) and demonstrated that sockeye salmon were not vulnerable to mortality. Chinook salmon were sensitive to 6PPDQ mortality at initial concentrations >25 μg/L, ∼10-fold greater than reported environmental measurements. In vitro , the coho salmon cell line CSE-119 was acutely sensitive to 6PPDQ (metabolic EC 50 = 7.9 μg/L, cytotoxicity EC 50 = 6.1 μg/L). Analogous Chinook (CHSE-214) and sockeye salmon (SSE-5) cell lines were nonresponsive in both assays, and rainbow trout RTG-2 cells began showing metabolic effects at 68 μg/L (EC 5 ). Recreation of species-specific 6PPDQ sensitivity in vitro implicates conserved modes of action in CSE-119 that could be utilized for mechanistic studies of 6PPDQ toxicity and screening of other PPD transformation products.

Environmental Science and Technology Letters↗

USGS2004

2,3,7,8-TCDD effects on visual structure and function in swim-up rainbow trout

An understanding of mechanisms of contaminant effects across levels of biological organization is essential in ecotoxicology if we are to generate predictive models for population-level effects. We applied a suite of biochemical, histological, and behavioral end points related to visual structure and function and foraging behavior to evaluate effects of 2,3,7,8-Tetrachlorodibenzo- p -dioxin (TCDD) on swim-up rainbow trout. We detected a dose-dependent decrease in densities of retinal ganglion cells (RGC), key retinal neurons that link the eye with the brain. These changes resulted in corresponding deficits in visual/motor function including reductions in visual acuity and in scotopic and photopic thresholds due to TCDD. The loss of RGCs suggests an increase in convergence of synapses from photoreceptors to RGCs as a cellular mechanism for the visual deficits. Dose-dependent increases in immunohistochemical detection of CYP1A protein in the vasculature of the brain and eye choroid was proportional with decreased ganglion cell densities in the retina. TCDD-induced AHR-regulated effects on these tissues might be involved in the detected decrease in ganglion cell densities. Prey capture rate decreased after TCDD exposure only at the highest treatment groups evaluated. Collectively, these results show that TCDD causes biochemical and structural changes in the eye and brain of rainbow trout that are associated with behavioral deficits leading to decreased individual fitness.

Environmental Science & Technology↗