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Effects of a low-lipid diet on the gut microbiome and head kidney transcriptome of juvenile Chinook Salmon

Objective Pacific salmon Oncorhynchus spp. reared in production hatcheries are typically fed high-lipid, energy-dense diets to achieve large size and high body condition prior to release. In contrast, juveniles in natural environments tend to consume low-lipid, high-protein diets, and fish reared for research or conservation purposes are sometimes fed diets that are formulated to mimic natural diets and promote wild-like phenotypes. Understanding how these alternative diets affect fish health beyond growth and body condition could ultimately contribute to improving hatchery fish fitness. Methods In this work, we evaluated changes in the fecal microbiome and gene expression of juvenile Chinook Salmon O. tshawytscha on a standard high-lipid hatchery diet versus a low-lipid diet formulated to mimic the nutrition profile of natural-origin fish. To evaluate the time scale at which diet alters the fecal microbiome, we collected longitudinal samples over a 12-week period and switched the diets of a subset of fish twice during the experiment. We used 16S ribosomal RNA gene amplicon sequencing to characterize fecal microbiome differences between fish on the two diets as well as hatchery-reared fish at a production hatchery, hatchery fish that had been captured after release into a stream, and natural-origin, stream-reared fish of similar ages. Additionally, we conducted RNA sequencing on head kidney samples from laboratory-reared fish to evaluate changes in gene expression in this important immune organ. Results We found that the low-lipid diet and the hatchery diet resulted in microbiomes that differed from the microbiome of natural-origin fish and from each other and that diet-driven changes to the microbiome could occur in under 14 d. The low-lipid diet did not result in a microbiome that resembled the microbiome of naturally produced fish. Instead, the low-lipid diet resulted in a microbiome community that was distinct from those of fish reared on the hatchery diet and fish sampled from the wild. The RNA sequencing results indicated differential enrichment of pathways related to immunity, metabolism, and hormone synthesis between fish that were fed the two experimental diets. Conclusions The results suggest that additional environmental factors influence the microbiome more strongly than diet formulation or that the low-lipid diet has a smaller effect on the microbiome than a natural, ­invertebrate-based diet. Given that the gut microbiome and systemic immune function contribute significantly to disease resistance, our findings highlight the importance of understanding how diets fed to fish in captivity may affect fish health beyond growth and body condition metrics.

Journal of Aquatic Animal Health

Geophysical identification of potential groundwater – surface water interactions in the Bonita Peak Mining District, San Juan County, Colorado

This report includes the results and interpretation of geophysical investigations to aid in the location, identification, and conceptual site model (CSM) development of the near surface groundwater and surface water hydrology of the Upper Cement Creek and California Gulch of the Animas Rivers within the Bonita Peak Mining Districts (BPMD). Mine influenced water (MIW) from numerous mine adits within the BPMD has altered the geochemistry and ecology of these ground and surface waters. Many studies of varying scales have investigated the geology, hydrogeology, geochemistry, and aqueous chemistry of these adits and impacted areas. Field investigations using non-invasive geophysical methods were conducted in the summer of 2019 to aid in identifying potential groundwater inputs to these streams and further the CSM of the near surface within and along these streams. Two field deployments were completed in August and September of 2019. In August 2019, Phase 1 was completed using multifrequency electromagnetic induction (EMI) and thermal infrared imaging (TIR) to measure the bulk electrical conductivity, magnetic susceptibility, and the temperature response at known and suspected groundwater seeps into the Upper Cement Creek and California Gulch stretches of these alpine streams. Results and interpretation from Phase 1 were used to co-locate stretches of these streams for further detailed fiber optic distributed temperature system (FODTS) monitoring of the temperature dynamics for submeter identification of potential groundwater seeps during Phase 2. Phase 2 included additional EMI measurements, FODTS measurements, and repeated some Phase 1 measurements for improved data quality. All these data were used for the combined qualitative interpretation identifying seep locations. The data from both Phase 1 and Phase 2 are available in the USGS data release at: https://www.sciencebase.gov/catalog/item/5f18a30082cef313ed84879b. These data include detailed near-surface electrical conductivity and magnetic susceptibility mapping, discrete TIR imaging, and several kilometers of FODTS data. These data were georeferenced and used to identify potential groundwater seeps into these streams. The results indicate a complex near surface geophysical and hydrogeologic regime. Seeps are classified and spatially correlated to the EMI, TIR, and geochemical sampling results. This report is a thorough compilation of the geophysical field efforts in the summer of 2019, the data processing, and interpretations of these data. These data alone should not be used for final interpretations of the complex hydrogeology within these alpine systems. Rather, these data should be combined with other known data from various investigations to further refine the CSM and guide decisions for future investigations, watershed protective measures, and other site decisions. Geographical information system (GIS) layers of these results are available to project managers for such integration and combined interpretations.

Colorado

Water-quality assessment of the Ozark Plateaus study unit, Arkansas, Kansas, Missouri, and Oklahoma — Nutrients, bacteria, organic carbon, and suspended sediment in surface water, 1993-95

Nutrient, bacteria, organic carbon, and suspended- sediment samples were collected from 1993-95 at 43 surface-water-quality sampling sites within the Ozark Plateaus National Water- Quality Assessment Program study unit. Most surface-water-quality sites have small or medium drainage basins, near-homogenous land uses (primarily agricultural or forest), and are located predominantly in the Springfield and Salem Plateaus. The water-quality data were analyzed using selected descriptive and statistical methods to determine factors affecting occurrence in streams in the study unit. Nitrogen and phosphorus fertilizer use increased in the Ozark Plateaus study unit for the period 1965-85, but the application rates are well below the national median. Fertilizer use differed substantially among the major river basins and physiographic areas in the study unit. Livestock and poultry waste is a major source of nutrient loading in parts of the study unit. The quantity of nitrogen and phosphorus from livestock and poultry wastes differed substantially among the river basins of the study unit's sampling network. Eighty six municipal sewage-treatment plants in the study unit have effluents of 0.5 million gallons per day or more (for the years 1985-91). Statistically significant differences existed in surface-water quality that can be attributed to land use, physiography, and drainage basin size. Dissolved nitrite plus nitrate, total phosphorus, fecal coliform bacteria, and dissolved organic carbon concentrations generally were larger at sites associated with agricultural basins than at sites associated with forested basins. A large difference in dissolved nitrite plus nitrate concentrations occurred between streams draining basins with agricultural land use in the Springfield and Salem Plateaus. Streams draining both small and medium agricultural basins in the Springfield Plateau had much larger concentrations than their counterparts in the Salem Plateau. Drainage basin size was not a significant factor in affecting total phosphorus, fecal coliform bacteria, or dissolved organic carbon concentrations. Suspended-sediment concentrations generally were small and indicative of the clear water in streams in the Ozark Plateaus. A comparison of the dissolved nitrite plus nitrate, total phosphorus, and fecal coliform data collected at the fixed and synoptic sites indicates that generally the data for streams draining basins of similar physiography, land-use setting, and drainage basin size group together. Many of the variations are most likely the result of differences in percent agricultural land use between the sites being compared or are discharge related. The relation of dissolved nitrite plus nitrate, total phosphorus, and fecal coliform concentration to percent agricultural land use has a strong positive 2 Water-Quality Assessment-Nutrients, Bacteria, Organic Carbon, and Suspended Sediment in Surface Water, 1993-95 correlation, with percent agricultural land use accounting for between 42 and 60 percent of the variation in the observed concentrations.

Arkansas, Kansas, Missouri, Oklahoma

Unlocking ecological insights from sub-seasonal visible-to-shortwave infrared imaging spectroscopy: The SHIFT campaign

We stand at the threshold of a transformative era in Earth observation, marked by space-borne visible-to-shortwave infrared (VSWIR) imaging spectrometers that promise consistent global observations of ecosystem function, phenology, and inter- and intra-annual change. However, the full value of repeat spectroscopy, the information embedded within different temporal scales, and the reliability of existing algorithms across diverse ecosystem types and vegetation phenophases have remained elusive due to the absence of suitable sub-seasonal spectroscopy data. In response, the Surface Biology and Geology (SBG) High-Frequency Time Series (SHIFT) campaign was initiated during late February 2022 in Santa Barbara County, California. SHIFT, designed to support NASA's SBG mission, addressed mission scoping, scientific advancement, applications development, and community building. This ambitious endeavor included weekly Airborne Visible InfraRed Imaging Spectrometer-Next Generation (AVIRIS-NG) imagery acquisitions for 13 weeks (spanning February 24 to May 29, 2022), accompanied by coordinated terrestrial vegetation and coastal aquatic data collection. We describe the rich datasets collected and illustrate how the complex sub-seasonal patterns of change can be linked to biological science and applications, surpassing insights from multispectral observations. Leveraging open-source processing methods and cloud-based analysis tools, the SHIFT campaign showcases the readiness of the scientific community to harness ecological insights from remotely sensed hyperspectral time series. We provide an overview of SHIFT's goals, data collections, preliminary results, and the collaborative efforts of early career scientists committed to unlocking the transformative potential of high-frequency time series data from space-borne VSWIR imaging spectrometers.

Ecosphere

Determining Volcanic Risk in Auckland (DEVORA) Research Programme—A transdisciplinary approach to address the challenge of distributed volcanism in an urban environment

The Determining Volcanic Risk in Auckland (DEVORA) Research Programme was launched in 2008 to address the challenges associated with monogenetic volcanism in an urban setting and to enhance volcanic risk management in Tāmaki Makaurau Auckland in Aotearoa New Zealand. It is a multi-agency, increasingly transdisciplinary (defined here as research that transcends traditional disciplinary boundaries by integrating diverse types of knowledge, perspectives, and methods from academic and non-academic participants to create novel solutions to complex problems), and collaborative research program jointly led by Waipapa Taumata Rau University of Auckland and Earth Sciences New Zealand (ESNZ; formerly GNS Science), with core funding from Natural Hazards Commission Toka Tū Ake (NHC; formerly the Earthquake Commission, EQC) and Te Kaunihera o Tāmaki Makaurau Auckland Council (AC). The primary research focus of DEVORA is to investigate the geologic history, volcanic hazards, and risk posed by the basaltic intraplate Auckland Volcanic Field. Disruption from ash fall and gas from other Aotearoa New Zealand volcanoes is also considered. DEVORA’s work to explore exposure and vulnerability in Tāmaki Makaurau Auckland is also useful for assessing risks from other non-volcanic natural hazards, such as seismic and tsunami hazards. The greater Tāmaki Makaurau Auckland region has an ethnically and socio-economically diverse population of approximately 1.7 million, representing about one-third of the Aotearoa New Zealand population, and hosts critical infrastructure of national significance. The size and nature of the populace, consequential economic base, and important infrastructure within Tāmaki Makaurau Auckland mean that the effects of a volcanic eruption would be felt nationally, including through the disruption of air travel to Aotearoa New Zealand. The hazards from such an eruption could potentially affect hundreds of thousands of people, businesses, and lifelines (critical infrastructure). A considerable challenge for emergency and risk managers is the monogenetic nature of the volcanic field. It is not known where or when the next eruption will occur, how much warning we may get before an eruption, nor how an eruption and its effects might unfold. In this contribution, we highlight the concept and collaborative intent of the DEVORA Programme and show how it has evolved over the 16 years since its inception. We describe how DEVORA has unified more than 100 researchers (including more than 50 graduate students) and numerous stakeholders to address key issues facing Tāmaki Makaurau Auckland and describe how research findings are being implemented into policy and communicated to stakeholder agencies and the public. We also illustrate the broader influence of the DEVORA Programme and provide some learnings that might benefit others embarking on similar integrated projects, especially those focused on distributed volcanism in and near populated areas.

Auckland

Federal lands greenhouse gas emissions and sequestration in the United States: Estimates for 2005–22

In 2016, the Secretary of the U.S. Department of the Interior requested that the U.S. Geological Survey (USGS) produce a publicly available and annually updated database of estimated greenhouse gas emissions associated with the extraction and use of fossil fuels from Federal lands. The first report in this series included emissions estimates from 2005 to 2014 and were reported for 29 States and two offshore areas. Native American and Tribal lands were not included in that analysis. This report recalculates those previous years (2005–14) with updated data and methods and extends the estimates to 2022. Nationwide emissions from fossil fuels produced on Federal lands in 2022 were 1,081.2 million metric tons of carbon dioxide equivalent (MMT CO 2 Eq.) for CO 2 , 33.4 MMT CO 2 Eq. for methane (CH 4 ), and 4.3 MMT CO 2 Eq. for nitrous oxide (N 2 O). Compared to 2005, the 2022 totals represent decreases in emissions for all three greenhouse gases (by 17 percent for CO 2 , 37 percent for CH 4 , and 30 percent for N 2 O). Emissions from fossil fuels produced on Federal lands represent, on average, 21.8 percent of U.S. emissions for CO 2 , 6.1 percent for CH 4 , and 1.3 percent for N 2 O over the 18 years included in this estimate. The trends and relative magnitudes of the greenhouse gas emissions estimated are roughly parallel to the Federal lands fossil fuel production volumes. In 2021, Federal lands of the conterminous United States stored 70,424 MMT CO 2 Eq. in terrestrial ecosystems. Soils stored most of the terrestrial ecosystem carbon (66 percent), followed by live vegetation (25 percent), deadwood (5 percent), and litter (4 percent). Carbon sequestration on Federal lands was highly variable over time, owing primarily to interannual variability in climate and weather, and variability in land use and land cover (LULC) change and disturbances, among these are wildfires and logging. Between 2005 and 2021, Federal lands sequestered an average of 83 MMT CO 2 Eq./yr. By subtracting the cumulative effects of LULC and disturbance-related CO 2 losses to the atmosphere from the total, we estimate that ecosystems at the national level sequestered CO 2 at an annual mean rate of 17 MMT CO 2 Eq./yr in a term called the net ecosystem exchange (NEE). This annual NEE sequestration value represents about 1.4 percent of average fossil fuel emissions over the same period. The USGS estimates presented in this report represent an accounting for the emissions resulting from fossil fuel extraction on Federal lands and the end-use combustion of those fuels, as well as for the sequestration of carbon in terrestrial ecosystems on Federal lands. A combined net CO 2 emissions estimate, which is the difference between the emitted and sequestered CO 2 from both the fossil fuel and ecosystems estimates, provides context for evaluating the greenhouse gas contributions of activities on these lands. The estimates included in this report can provide context for future energy decisions, as well as a basis to track change in the future.

Scientific Investigations Report

Ground-water flow in the surficial aquifer system and potential movement of contaminants from selected waste-disposal sites at Naval Station Mayport, Florida

Ground-water flow through the surficial aquifer system at Naval Station Mayport near Jacksonville, Florida, was simulated with a two-layer finite-difference model as part of an investigation conducted by the U.S. Geological Survey. The model was calibrated to 229 water-level measurements from 181 wells during three synoptic surveys (July 17, 1995; July 31, 1996; and October 24, 1996). A quantifiable understanding of ground-water flow through the surficial aquifer was needed to evaluate remedial-action alternatives under consideration by the Naval Station Mayport to control the possible movement of contaminants from sites on the station. Multi-well aquifer tests, single-well tests, and slug tests were conducted to estimate the hydraulic properties of the surficial aquifer system, which was divided into three geohydrologic units�an S-zone and an I-zone separated by a marsh-muck confining unit. The recharge rate was estimated to range from 4 to 15 inches per year (95 percent confidence limits), based on a chloride-ratio method. Most of the simulations following model calibration were based on a recharge rate of 8 inches per year to unirrigated pervious areas. The advective displacement of saline pore water during the last 200 years was simulated using a particle-tracking routine, MODPATH, applied to calibrated steady-state and transient models of the Mayport peninsula. The surficial aquifer system at Naval Station Mayport has been modified greatly by natural and anthropogenic forces so that the freshwater flow system is expanding and saltwater is being flushed from the system. A new MODFLOW package (VAR1) was written to simulate the temporal variation of hydraulic properties caused by construction activities at Naval Station Mayport. The transiently simulated saltwater distribution after 200 years of displacement described the chloride distribution in the I-zone (determined from measurements made during 1993 and 1996) better than the steady-state simulation. The advective movement of contaminants from selected sites within the solid waste management units to discharge points was simulated using MODPATH. Most of the particles were discharged to the nearest surface-water feature after traveling less than 1,000 feet in the ground-water system. Most areas within 1,000 feet of a surface-water feature or storm sewer had traveltimes of less than 50 years, based on an effective porosity of 40 percent. Contributing areas, traveltimes, and pathlines were identified for 224 wells at Naval Station Mayport under steady-state and transient conditions by back-tracking a particle from the midpoint of the wetted screen of each well. Traveltimes to contributing areas that ranged between 15 and 50 years, estimated by the steady-state model, differed most from the transient traveltime estimates. Estimates of traveltimes and pathlines based on steady-state model results typically were 10 to 20 years more and about twice as long as corresponding estimates from the transient model. The models differed because the steady-state model simulated 1996 conditions when Naval Station Mayport had more impervious surfaces than at any earlier time. The expansion of the impervious surfaces increased the average distance between contributing areas and observation wells.

Florida

Streamflow, base flow, and ground-water recharge in the Housatonic River basin, western Massachusetts and parts of eastern New York and northwestern Connecticut

Streamflows for selected flow durations from 1 to 99 percent and the August median streamflows were estimated for 11 long-term streamflow-gaging stations in and near the study area. Estimates of streamflow and associated standard errors were determined for selected flow durations from 50 to 99 percent and the August median streamflows for 21 low-flow partial-record stations and for selected flow durations from 1 to 99 percent and the August median streamflows for two partial-record stations and seven short-term discontinued streamflow-gaging stations. Median streamflows per square mile for the 10-, 50-, and 90-percent flow durations and the August median streamflows were 3.90, 1.01, 0.185, and 0.248 cubic feet per second per square mile. Streamflows per square mile at selected flow-duration discharges between 1 and 99 percent at the 41 stations were related to basin characteristics to explain differences in streamflow characteristics. Basin characteristics included basin elevations, extent of stratified-drift deposits, land use, aspect, and underlying bedrock geology types. Most streamflow differences were positively correlated to basin elevation differences, most likely because precipitation increases with elevation, and to stratified-drift deposits, which allow more precipitation to recharge the ground water and to discharge later than do till and bedrock deposits. Mean base flow was computed from continuous records of daily mean discharge at 11 long-term streamflow-gaging stations in and near the study area. Mean annual base flow ranged from 13.4 to 24.5 inches per year. Minimum annual base flow ranged from 45 to 72 percent of mean annual rates at the 11 long-term stations, and the ratio of base flow to streamflow (base-flow index) ranged from 0.55 to 0.80. Base-flow durations between 1 and 99 percent were calculated from streamflow records at the 11 long-term streamflow-gaging stations. Base flow accounted for 45.5 to 85.0 percent of total annual streamflow at the 1- and 99-percent flow durations. Ground-water-recharge rates were computed from continuous records of daily mean discharge at 11 long-term streamflow-gaging stations in and near the study area. Mean annual ground-water-recharge rates ranged from 17.5 to 22.4 inches per year at 10 of the 11 long-term stations. Mean annual ground-water-recharge rates ranged from 2 to 7 inches per year higher than base flow. Minimum annual ground-water-recharge rates ranged from 48 to 72 percent of mean annual ground-water-recharge rates. Mean annual potential ground-water recharge was estimated from monthly climatological data collected at six climatological stations in and near the study area. Mean potential ground-water recharge ranged from about 17.9 to 28.9 inches per year, with a median value of 22.6 inches per year. This median value compares well to that calculated by use of streamflow records at the 11 streamflow-gaging stations (20.0 inches per year). Streamflows per square mile for the 10-, 50-, and 90-percent flow durations at stations in and near the study area were similar to those computed for other unregulated long-term continuous streamflow-gaging stations in central and eastern Massachusetts. Base-flow and ground-water-recharge rates in the study area compared closely to results from other studies in southeastern Massachusetts and Rhode Island, which were based on the same computational methods.

Connecticut, Massachusetts, New York

Status and understanding of groundwater quality in the San Joaquin Valley Kern County subbasin domestic-supply aquifer study unit, 2022—California GAMA Priority Basin Project

The quality of water accessed by domestic wells (here referred to as domestic groundwater resources) in the San Joaquin Valley Kern County subbasin (basin number 5-022.14) was assessed as part of the California Groundwater Ambient Monitoring and Assessment (GAMA) Program Priority Basin Project (GAMA-PBP), in cooperation with the California State Water Resources Control Board. Kern County is at the southern end of the San Joaquin Valley in California, and about 30,000 residents are estimated to use privately owned domestic wells for drinking water. Domestic wells typically draw from shallower parts of the aquifer system than public-supply wells and can be more vulnerable to effects from surface activities. Kern County is host to a highly productive agricultural industry, with Bakersfield as the main urban center. The Kern River runs through Bakersfield from the southern Sierra Nevada and intersects the Kern Water Bank, one of the largest groundwater banking operations in California, at the Kern River Intertie. The section of the Kern River running through the Kern Water Bank is dry most years. Kern County also encompasses some of the most productive oil and gas basins in California, with extensive underground and surface disposal of oil-field wastewater. This study was based on data collected from 33 sites sampled by the U.S. Geological Survey for the GAMA-PBP in 2022. To provide context for the water quality assessment, measured concentrations were compared to regulatory and non-regulatory health-based and aesthetic benchmarks. A grid-based method was used to estimate the proportions of the groundwater resources used for domestic-supply wells that have water-quality constituents below (low relative concentration), approaching (moderate relative concentration), or above (high relative concentration) benchmark concentrations. At least one measured constituent with a regulatory benchmark was categorized as having a high relative concentration in 72 percent of the aquifer area used for domestic groundwater resources. Inorganic constituents were detected at high concentrations in 45 percent of the domestic groundwater resources, and the constituents detected above regulatory benchmarks were arsenic, nitrate, and uranium. At least one organic constituent was detected at high concentrations in 41 percent of the domestic groundwater resources, and the constituents exceeding regulatory benchmarks were the fumigants 1,2,3-trichloropropane (1,2,3-TCP), 1,2-dibromo-3-chloropropane (dibromochloropropane [DBCP]), 1,2-dibromoethane (EDB), and the per-and polyfluoroalkyl substance (PFAS) perfluorooctanesulfonate. The disinfection by-product chloroform, the fumigant 1,2-dichloropropane, the herbicides atrazine and hexazinone, and the herbicide degradates 2-chloro-6-ethylamino-4-amino-s-triazine, 2-chloro-4,6-diamino-s-triazine, 4-hydroxychlorothalonil, and metolachlor sulfonic acid were detected in more than 10 percent of domestic groundwater resources, but concentrations did not exceed regulatory benchmarks. Land use, groundwater age (fraction of modern water and mean age), and geochemical environment (oxic or anoxic conditions, pH, alkalinity) were associated with the distribution of high relative concentrations of inorganic and organic constituents. Young, oxygenated water is recharged along the Kern River and adjacent recharge ponds, or as irrigation water in the agricultural areas. High concentrations of nitrate and volatile organic compounds occurred in the oxic water in urban and agricultural areas. The fumigants 1,2,3-TCP, DBCP, and EDB were reported throughout the agricultural areas, whereas chloroform, tetrachloroethene, and PFAS were associated with urban land use. High uranium concentrations were associated with young, modern groundwater in agricultural areas with low pH and high bicarbonate. Total dissolved solids increased with distance from the Kern River, as the contributions of fresh, oxic water decreased. High concentrations of arsenic were present in older anoxic or alkaline groundwater away from areas of recharge. Overall, groundwater age, redox conditions, and the source of recharge as a result of different land uses contribute to large aquifer-scale portions of domestic groundwater resources that exceed health-based benchmarks for nitrate, uranium, and fumigant concentrations.

California

Numerical model of the groundwater-flow system near the southeastern part of Puget Sound, Washington

Groundwater flow in the active model area (AMA) was simulated using a groundwater-flow model. A steady-state model version of the model simulates equilibrium conditions, and a transient model version simulates monthly variability. The model corresponds to the physical and temporal dimensions of the conceptual model and groundwater budget. The steady-state model version represents average conditions for an 11-year period (January 1, 2005–December 31, 2015), and the transient model represents monthly hydrologic variability within that period. The 13-layer model was constructed using MODFLOW-NWT with a uniformly spaced grid consisting of 416 rows, 433 columns, and cells with a horizontal dimension of 500 feet (ft) on a side. The model was calibrated to measured values of water levels in wells and lakes and estimated base flow for selected streamflow measurement stations, commonly referred to as streamgages. Model calibration was accomplished using a combination of manual and automatic methods, including the Model-Independent Parameter Estimation (PEST) program that adjusted model input parameters with the aim of minimizing the difference between estimated and model-simulated values of hydraulic head and base flow. Model boundary conditions consist of all simulated groundwater inflow to and outflow from the AMA. For example, a stream reach that simulates a gain from or loss to groundwater is a boundary condition that allows water to exit or enter, respectively, the groundwater system. Other boundary conditions include springs, seeps, precipitation recharge, groundwater exchange with lakes and Puget Sound, and groundwater pumping. A comparison of the estimated groundwater budget to that simulated by the steady-state model version indicates that the relative percentages of total inflow or total outflow for six major categories of boundary conditions are similar for the two budgets. The model was used to simulate three suites of scenarios of potential drought and water-use changes. Scenario 1 suite consisted of the steady-state model version that was run with 0, 15, 20, and 25 percent reduction of precipitation recharge to assess the corresponding reductions in base flow with decreasing recharge. The last simulation for the scenario 1 suite consisted of the transient model version simulating 3 years of consecutive seasonal drought, defined by the months of May through September, to assess the corresponding base-flow reductions. Scenario 2 suite consisted of the steady-state model version with all simulated groundwater use removed, compared with a simulation that includes current groundwater use to evaluate changes to potentiometric surfaces and base flows. Scenario 3 suite consisted of a transient model version of the model that simulated pumping increases for four different categories of water-supply wells (compared to no pumping increases) to evaluate resulting reductions in base flow. Although, these scenarios provide examples of model applications and useful insights, many other scenarios could be simulated. A description of how to download the model is described in the body of this report. Uncertainty is associated with most model inputs. Groundwater levels, lake levels, and land-surface altitudes are relatively certain; other model inputs are far less certain, including precipitation recharge, base flow, hydraulic properties, water use, and the three-dimensional structure of subsurface hydrogeologic units. Models are useful not because of high levels of accuracy of all model inputs, but because they combine the best information and estimates available, thereby providing the best predictions available related to physical processes. The model described in this report simulates groundwater flow on a regional scale, which has inherent limitations for simulating hydrologic scenarios at local scales. Model structures and inputs were generalized to be consistent with this regional scale. For example, the actual groundwater system has much greater heterogeneity of hydraulic conductivity than is possible within the model’s degrees of freedom. Variations in hydraulic gradients over distances less than 500 ft cannot be simulated. The distances between model features, such as a pumping well and a stream, must be placed at 500-ft intervals and are co-located if both features are within the same model cell.

Washington

Timing of hydrothermal alteration and Au-Sb-W mineralization, Stibnite-Yellow Pine district, Idaho

The Stibnite-Yellow Pine district of central Idaho was mined from the early 1900s until the 1990s, extracting gold, antimony, tungsten, and mercury from veins and disseminated and replacement ores in mountainous terrain along the headwaters of the Salmon River. Mining during the two World Wars supplied critical antimony and tungsten to the war efforts. Recent exploration has delineated mineral resources of over 187 metric tons Au, 274 metric tons Ag, and 93,000 metric tons Sb. Mineralization is hosted in Cretaceous Idaho batholith granitic rocks and a sequence of Neoproterozoic to Paleozoic metasedimentary strata of carbonate and siliciclastic compositions. Historical studies outlined some of the complex paragenesis but debated the absolute age of mineralization. New petrographic and geochronologic work documents a sequence of five hydrothermal events in the Stibnite-Yellow Pine district. Event 1 is related to Cretaceous magmatic and hydrothermal activity and includes events ranging in age from 86 to 75 Ma, including sparse quartz-molybdenite veins dated at 86 Ma. Disseminated gold mineralization of event 2 is associated with sericitic alteration and sulfidation of igneous biotite and replacement of plagioclase by potassium feldspar, largely in granodiorite. Gold is present in zoned arsenian pyrite in both disseminated ores and in crosscutting carbonate-quartz veins containing pyrite and arsenopyrite. The large Yellow Pine deposit, localized at a dilatant bend in the Meadow Creek fault, hosts such disseminated and vein gold. Event 2 is interpreted as the major gold-forming event; 40 Ar/ 39 Ar ages of sericite and potassium feldspar alteration range, respectively, from 70 to 59 and 66 to 56 Ma. The long span is interpreted to reflect the age of gold mineralization and local overprinting by event 3. A narrower range from 66 to 61 Ma is interpreted to date the peak of gold mineralization and alteration. Event 3, tungsten mineralization with scheelite, is texturally later than event 2 gold and localized along the Meadow Creek structure. Event 3 scheelite has been dated by isotope dilution-thermal ionization mass spectrometry (ID-TIMS) and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) U-Pb methods at 57 Ma. Event 4, best developed in the West End area, includes gold-silver–bearing quartz-carbonate-pyrite veins and breccias with epithermal textures and potassium feldspar alteration envelopes. Adularia from event 4 yields 40 Ar/ 39 Ar plateau ages of 52 to 51 Ma. Event 5 antimony and mercury mineralization consists of stibnite veins and breccia cements at the Yellow Pine and Hangar Flat deposits as well as cinnabar veins and replacements at the peripheral Fern and Hermes deposits; it is constrained by an LA-ICP-MS U-Pb date on scheelite (ca. 47 Ma) intergrown with stibnite. Minor propylitic and argillic alteration is evident in 47 Ma igneous dikes, which do not contain economic mineralization. The Au-Sb-W ores in the Stibnite-Yellow Pine mining district formed over an extended time period from about 70 to 45 Ma in multiple pulses that were localized along the Meadow Creek fault zone. Each event corresponds to episodes of magmatism and/or hydrothermal activity in the region. Insignificant event 1 skarn and molybdenum mineralization is similar in age to the Thompson Creek porphyry molybdenum deposit in central Idaho. Event 2 gold mineralization occurred during a magmatic gap in central Idaho but was synchronous with magmatism in the Bitterroot lobe further north; event 2 is similar in age to orogenic gold-arsenic mineralization at the Beartrack mine in eastern Idaho. Event 3 scheelite mineralization coincides with tungsten mineralization at the Quartz Creek deposit, late magmatism in the Bitterroot lobe, and rapid exhumation of the Atlanta lobe of the Idaho batholith. Event 4 gold mineralization is coincident with the onset of regional Challis magmatism and extension. Event 5 antimony and mercury mineralization is time-equivalent to epithermal gold mineralization in the nearby Thunder Mountain volcanic field and the peak of Challis magmatism.

Idaho

Status and understanding of groundwater quality in the Mojave Basin Domestic-Supply Aquifer study unit, 2018—California GAMA Priority Basin Project

Groundwater quality in the western part of the Mojave Desert in San Bernardino County, California, was investigated in 2018 as part of the California State Water Resources Control Board Groundwater Ambient Monitoring and Assessment Program Priority Basin Project. The Mojave Basin Domestic-Supply Aquifer study unit (MOBS) region was divided into two study areas—floodplain and regional—to assess differences between the two major aquifers used for drinking water supply in the area. This assessment characterized the quality of ambient groundwater and not the quality of treated drinking water. The study included three components: (1) a status assessment, which characterized the quality of groundwater resources used for domestic drinking-water supply in the floodplain and regional study areas; (2) a brief understanding assessment, which evaluated factors that could potentially affect the quality of groundwater used by domestic wells in the region; and (3) a comparative assessment between the groundwater resources used by domestic wells and public-supply wells in the two study areas. The domestic-well assessment was based on data collected by the U.S. Geological Survey from 48 domestic wells in January–May 2018. The public-supply assessment was based on data for samples from 322 public-supply wells in 2008–18, either collected by the U.S. Geological Survey or compiled from the California State Water Resources Control Boards Division of Drinking Water publicly available database. Concentrations of water-quality constituents in ambient groundwater were compared to regulatory and non-regulatory benchmarks typically used by the State of California and Federal agencies as health-based or aesthetic standards for public drinking water. Relative concentrations, defined as the measured concentration divided by the benchmark concentration, were classified as high (greater than 1.0), moderate (greater than 0.5 for inorganic constituents or 0.1 for organic and special-interest constituents, and not high), or low (concentrations lower than moderate). The floodplain and regional study areas were divided into 15 and 35 grid cells, respectively, and grid-based methods were used to compute the areal proportions of the two study areas with high, moderate, or low relative concentrations of individual constituents and classes of constituents. For the domestic-supply assessment, one or more inorganic constituents with health-based benchmarks were detected at high relative concentrations in 58 percent of the regional study area and 13 percent of the floodplain study area. The inorganic constituents with health-based benchmarks detected at high relative concentrations in the regional study area were arsenic, chromium and hexavalent chromium, fluoride, adjusted gross alpha particle activity, uranium, molybdenum, strontium, and nitrate; only arsenic was detected at high relative concentrations in the floodplain study area. One or more inorganic constituents with secondary maximum contaminant level benchmarks were detected at high concentrations in 15 and 6.7 percent of the regional and floodplain study areas, respectively. The constituents detected at high relative concentrations in the regional study area were total dissolved solids, chloride, sulfate, and iron; only total dissolved solids and sulfate were detected at high relative concentrations in the floodplain study area. Organic constituents were not detected at moderate or high relative concentrations in either the regional or floodplain study areas. Volatile organic compounds were detected at low relative concentrations in 21 and 27 percent of the regional and floodplain study areas, respectively, and pesticides were detected at low relative concentrations in 9.1 and 20 percent of the regional and floodplain study areas, respectively. The only individual organic constituent detected in more than 10 percent of either study area was the trihalomethane trichloromethane. Total coliform bacteria were detected in 15 and 27 percent of the grid wells in the regional and floodplain study areas, respectively. The greater prevalence of high relative concentrations of many inorganic constituents in the regional study area compared to the floodplain area likely indicates the greater diversity of geologic material at depth in aquifer material and generally finer-grained alluvium compared to the floodplain study area combined with generally older groundwater that has had more contact time with aquifer materials. In general, trace element concentrations (1) increased with increasing groundwater age, (2) increased with distance from recharge sources in the mountains, and (3) increased with closer proximity to some types of geological units. In general, groundwater from domestic wells in the floodplain study area is young, with most samples containing a component of modern groundwater based on tritium and unadjusted carbon-14 activities, whereas groundwater from domestic wells in the regional study area generally is old, with most samples having unadjusted carbon-14 ages of 5,000–40,000 years. Public-supply wells in MOBS generally were deeper than domestic wells and presumably are in contact with older, more weathered alluvium that may have more mobile trace elements, such as arsenic or uranium. However, only 26 percent of the public-supply regional study area had high relative concentrations of inorganic constituents, compared to 58 percent for the domestic regional study area. The percentages of the public-supply and domestic floodplain study areas with high relative concentrations of inorganic constituents were 11 and 13 percent, respectively. The ages of groundwater used by public-supply and domestic wells in each study area were similar, which was not expected given the greater depth of the public-supply wells. Three potential factors may contribute to these results: (1) greater spatial footprint of domestic well network, which may result in domestic wells pumping groundwater from fractured bedrock or mineralized areas not used by public-supply wells; (2) greater pumping rates in public-supply wells, resulting in more water being withdrawn from coarse-grained, heterogeneous alluvium than finer-grained layers, which may have higher concentrations of (or more mobile) inorganic constituents; and (3) a greater degree of well management with public-supply wells, which may include pausing use of or decommissioning wells if treating or blending water is not feasible to lower constituent concentrations.

California

Assessing spatial variability of nutrients, phytoplankton, and related water-quality constituents in the California Sacramento–San Joaquin Delta at the landscape scale—2018 high resolution mapping surveys

Executive Summary This study examined the abundance and distribution of nutrients and phytoplankton in the tidal aquatic environments of the Sacramento–San Joaquin Delta (Delta) and Suisun Bay, comprising three spatial surveys conducted in May, July, and October of 2018 that used continuous underway high frequency sampling and measurements onboard a high-speed boat to characterize spatial variation across the extent of the Delta. The method used involves simultaneously collecting information about the concentration and spatial distribution of all major nutrient forms with analogous information about the major classes of phytoplankton and associated water-quality conditions. The results showed substantial variation across space and time, providing an unprecedented snapshot of the dynamic environmental processes that shape the ways nutrients interact with and affect aquatic habitats in the Delta. The purposes of this study were to improve our understanding of how hydrodynamics, landscape features, and aquatic primary productivity interact to drive nutrient cycling and transport in the Delta and to provide insights into the underlying processes most directly responsible for the conditions at the time of this study, and thus into the range of conditions that may be expected following the wide array of prospective future changes to the Delta. One major anticipated change at the time of this study was the planned upgrade to the Sacramento Regional Wastewater Treatment Plant, but the study also informs our understanding of potential effects from other changes to the Delta, such as those caused by other nutrient-management actions, flow actions, large-scale wetland restoration, drought, flood, levee failure, and changes to water management. Nutrient loading is the primary driver of nutrient concentrations in the Delta, but several other major drivers interact to shape their distribution and effects: geomorphology, hydrodynamics, landscape features, and aquatic productivity. Hydrodynamics affect timescales of transport and dilution of nutrient loads in the Delta. During transit through the system, channel geometry, tidal mixing, and water exports affect hydrodynamics in diverse ways that influence water-residence and transport times, thereby markedly affecting the range of times during which natural internal cycling can alter nutrient concentrations and forms. Channel geometry and location shape tidal energy and river currents into these observed dynamics. Interactions with Delta aquatic landscapes such as herbaceous tidal marsh, submerged aquatic vegetation, and large expanses of intertidal or subtidal sediments (all highly productive landscapes) exert demand on available nutrient supplies but can also simultaneously transform and generate nutrients. Finally, while phytoplankton require nutrients to sustain production and thus are a potential nutrient sink, the amount and form of nutrients also can influence the occurrence of harmful algal blooms (HABs) that adversely affect aquatic organisms as well as affect the occurrence of beneficial algal blooms that result in production of algae that are favorable for imperiled Delta pelagic aquatic food webs. The surveys revealed a complex mosaic of spatial variation, with nutrient concentrations varying from near zero to well above concentrations considered eutrophic; nutrient concentrations were more often related to the extent of hydrologic transport and mixing than to specific geographic locations or to specific landscape features. Similarly, the surveys identified phytoplankton abundance ranging from near detection to the level of large phytoplankton blooms, with large variation in phytoplankton community composition. Although the study occurred during a period of low bloom activity, phytoplankton productivity appeared to be the strongest potential sink for inorganic nutrients in the Delta, indicating that it is a larger control on nutrient concentrations and distribution than previously understood. Cycling and transformation within the water column only appeared to substantially lower total nutrient concentrations at the longest estimated transport timescales. Contrary to expectations, we did not observe substantial nutrient depletion near landscape-scale features such as open-water habitats, submerged aquatic vegetation beds, extensive wetlands, or exposed sediments, indicating that these habitat types did not act as major sinks for nutrients in the Delta during these surveys. These results indicated that nutrient reduction efforts may have the greatest effect on pelagic phytoplankton productivity in the more productive reaches of the Delta and estuary, but these effects are unlikely to be magnified by changes to nutrient loss within the Delta over conceivable changes in flow conditions, Delta water management actions, or large-scale wetland restoration activities. Nevertheless, local processes were shown to cause substantial loss, and thus integrating of nutrient effects with other indicators of aquatic habitat conditions will help inform planning future actions at specific sites. Finally, we note that the primary contribution of this study was intended to be the survey data themselves. Aside from the results highlighted in this report, the surveys are a benchmark against which future environmental change may be evaluated, including changes to nutrient management or water exports, drought, large-scale wetland restoration, and climate change. Further, although we highlight some of the main findings from the surveys in this report, the necessarily limited scope precludes examination of many topics for which these surveys may be highly informative. To facilitate the utility of these data to stakeholders, managers, and researchers, we have released the data online (Bergamaschi and others, 2020) and created an online data exploration portal ( https:​//ca.water​.usgs.gov/​bay-​delta/​2018-​delta-​wide-​mapping-​surveys.html ) where users may query the surveys in a variety of ways to test hypotheses, examine relationships, assess spatial trends, and download data. The data exploration portal is intended to be an immersive experience that allows users to gain greater understanding of the complex interactions that shape Delta aquatic environments. This report is intended as a companion to the portal, allowing the reader to challenge and further explore the highlighted findings. This study was a collaboration between the U.S. Geological Survey and the Delta Regional Monitoring Program, with additional funding provided from U.S. Geological Survey Cooperative Matching Funds Program.

California

Preliminary geologic map of the Sparta East, Sparta West, and parts of the Glade Valley and Whitehead 7.5-minute quadrangles, North Carolina and Virginia, and the epicentral area of the August 9, 2020, Mw 5.1 earthquake near Sparta, North Carolina

Introduction New bedrock and surficial geologic mapping in the Sparta East, Sparta West, and parts of the Glade Valley and Whitehead 7.5-minute quadrangles, North Carolina and Virginia, investigates the geologic framework and causative mechanisms of the August 9, 2020, Mw 5.1 earthquake near Sparta, North Carolina. The mapping documents (1) the coseismic surface rupture from the 2020 earthquake and related brittle structures in the bedrock; (2) the fault contact between the western Blue Ridge and eastern Blue Ridge; (3) lithostratigraphy in the Lynchburg Group, Ashe Metamorphic Suite, and Alligator Back Metamorphic Suite; (4) the nature of the contact between the Lynchburg Group, Ashe Metamorphic Suite, and Alligator Back Metamorphic Suite; and (5) surficial deposits.

North Carolina, Virginia