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Presence of selected chemicals of emerging concern in water and bottom sediment from the St. Louis River, St. Louis Bay, and Superior Bay, Minnesota and Wisconsin, 2010

The St. Louis Bay of Lake Superior receives substantial urban runoff, wastewater treatment plant effluent, and industrial effluent. In 1987, the International Joint Commission designated the St. Louis Bay portion of the lower St. Louis River as one of the Great Lakes Areas of Concern. Concerns exist about the potential effects of chemicals of emerging concern on aquatic biota because many of these chemicals, including endocrine active chemicals, have been shown to affect the endocrine systems of fish. To determine the occurrence of chemicals of emerging concern in the St. Louis River, the St. Louis Bay, and Superior Bay, the U.S. Geological Survey in cooperation with the Minnesota Pollution Control Agency and the Wisconsin Department of Natural Resources collected water and bottom-sediment samples from 40 sites from August through October 2010. The objectives of this study were to (1) identify the extent to which chemicals of emerging concern, including pharmaceuticals, hormones, and other organic chemicals, occur in the St. Louis River, St. Louis Bay, and Superior Bay, and (2) identify the extent to which the chemicals may have accumulated in bottom sediment of the study area. Samples were analyzed for selected wastewater indicators, hormones, sterols, bisphenol A , and human-health pharmaceuticals. During this study, 33 of 89 chemicals of emerging concern were detected among all water samples collected and 56 of 104 chemicals of emerging concern were detected in bottom-sediment samples. The chemical N,N -diethyl- meta -toluamide (DEET) was the most commonly detected chemical in water samples and 2,6-dimethylnaphthalene was the most commonly detected chemical in bottom-sediment samples. In general, chemicals of emerging concern were detected at a higher frequency in bottom-sediment samples than in water samples. Estrone (a steroid hormone) and hexahydrohexamethyl cyclopentabensopyran (a synthetic fragrance) were the most commonly detected endocrine active chemicals in water samples; beta -sitosterol (a plant sterol), estrone, and 4- tert -octylphenol (an alkylphenol) were the most commonly detected endocrine active chemicals in bottom-sediment samples. The greater detection frequency of chemicals in bottom-sediment samples compared to the detection frequency in water samples indicates that bottom sediment is an important sink for chemicals of emerging concern. At least one polycyclic aromatic hydrocarbon was detected in every sample; and in most samples, all nine polycyclic aromatic hydrocarbons included in analyses were detected. Bottom sediment collected from Superior Bay had the most polycyclic aromatic hydrocarbon detections of the sediment sampling locations.

Minnesota;Wisconsin

Ecological Requirements for Pallid Sturgeon Reproduction and Recruitment in the Lower Missouri River: A Research Synthesis 2005-08

This report provides a synthesis of results obtained between 2005 and 2008 from the Comprehensive Sturgeon Research Program, an interagency collaboration between the U.S. Geological Survey, Nebraska Game and Parks Commission, U.S. Fish and Wildlife Service, and the U.S. Army Corps of Engineers' Missouri River Recovery - Integrated Science Program. The goal of the Comprehensive Sturgeon Research Program is to improve fundamental understanding of reproductive ecology of the pallid sturgeon with the intent that improved understanding will inform river and species management decisions. Specific objectives include: *Determining movement, habitat-use, and reproductive behavior of pallid sturgeon; *Understanding reproductive physiology of pallid sturgeon and relations to environmental conditions; *Determining origin, transport, and fate of drifting pallid sturgeon larvae, and evaluating bottlenecks for recruitment of early life stages; *Quantifying availability and dynamics of aquatic habitats needed by pallid sturgeon for all life stages; and *Managing databases, integrating understanding, and publishing relevant information into the public domain. Management actions to increase reproductive success and survival of pallid sturgeon in the Lower Missouri River have been focused on flow regime, channel morphology, and propagation. Integration of 2005-08 Comprehensive Sturgeon Research Program research provides insight into linkages among flow regime, re-engineered channel morphology, and pallid sturgeon reproduction and survival. The research approach of the Comprehensive Sturgeon Research Program integrates opportunistic field studies, field-based experiments, and controlled laboratory studies. The field study plan is designed to explore the role of flow regime and associated environmental cues using two complementary approaches. An upstream-downstream approach compares sturgeon reproductive behavior between an upstream section of the Lower Missouri River with highly altered flow regime to a downstream section that maintains much of its pre-regulation flow variability. The upstream section also has the potential for an experimental approach to compare reproductive behavior in years with pulsed flow modifications ('spring rises') to years without. The reproductive cycle of the female sturgeon requires several years to progress through gonadal development, oocyte maturation, and spawning. Converging lines of evidence support the hypothesis that maturation and readiness to spawn in female sturgeon is cued many months before spawning. Information on reproductive readiness of shovelnose sturgeon indicates that sturgeon at different locations along the Lower Missouri River between St. Louis and Gavins Point Dam are all responding to the same early cue. Although not a perfect surrogate, the more abundant shovelnose sturgeon is morphologically, physiologically, and genetically similar to pallid sturgeon, and thereby provides a useful comparative model for the rarer species. Day length is the likely candidate to define a temporal spawning window. Within the spawning window, one or more additional, short-term, and specific cues may serve to signal ovulation and release of gametes. Of three potential spawning cues - water temperature, water discharge, and day of year - water temperature is the most likely proximate cue because of the fundamental physiological role temperature plays in sturgeon embryo development and survival, and the sensitivity of many fish hormones to temperature change. It also is possible that neither temperature nor discharge is cueing spawning; instead, reproductive behavior may result from the biological clock advancing an individual fish's readiness to spawn day after day through the spawning period until the right moment, independent of local environmental conditions. Separation of the individual effects of discharge events, water temperature, and other possible factors, such as proximity to male

Scientific Investigations Report

Streamflow gain-loss characteristics of Elkhead Creek downstream from Elkhead Reservoir near Craig, Colorado, 2009

The U.S. Geological Survey (USGS), in cooperation with the Colorado Water Conservation Board, the Upper Colorado River Endangered Fish Recovery Program (UCREFRP), Colorado Division of Water Resources, and City of Craig studied the gain-loss characteristics of Elkhead Creek downstream from Elkhead Reservoir to the confluence with the Yampa River during August through October 2009. Earlier qualitative interpretation of streamflow data downstream from the reservoir indicated that there could be a transit loss of nearly 10 percent. This potential loss could be a significant portion of the releases from Elkhead Reservoir requested by UCREFRP during late summer and early fall for improving critical habitat for endangered fish downstream in the Yampa River. Information on the gain-loss characteristics was needed for the effective management of the reservoir releases. In order to determine streamflow gain-loss characteristics for Elkhead Creek, eight measurement sets were made at four strategic instream sites and at one diversion from August to early October 2009. An additional measurement set was made after the study period during low-flow conditions in November 2009. Streamflow measurements were made using an Acoustic Doppler Velocimeter to provide high accuracy and consistency, especially at low flows. During this study, streamflow ranged from about 5 cubic feet per second up to more than 90 cubic feet per second with step increments in between. Measurements were made at least 24 hours after a change in reservoir release (streamflow) during steady-state conditions. The instantaneous streamflow measurements and the streamflow volume comparisons show the reach of Elkhead Creek immediately downstream from Elkhead Reservoir to the streamflow-gaging station 09246500, Elkhead Creek near Craig, CO, is neither a gaining nor losing reach. The instantaneous measurements immediately downstream from the dam and the combined measurements of Norvell ditch plus streamflow-gaging station 09246500 are mostly within the plus or minus 5-percent measurement error of each other. The variability of data is such that sometimes the streamflow is greater upstream than downstream and sometimes the streamflow is greater downstream than upstream. Streamflow volumes were calculated for multiple time periods as determined by a change in release from the reservoir. Streamflow volumes were greater downstream than upstream for all but one time period. The predominance of greater streamflows downstream is due to the difference between the USGS instantaneous measurements and record computation with the Supervisory Control and Data Acquisition (SCADA) record at the dam. Immediately following an increase in streamflow from the reservoir, the downstream volume was smaller than the upstream volume, but this was an artifact of the traveltime between the two sites and possibly small amounts of water entering the streambank. Traveltimes were shorter at higher streamflows and when streamflow was increasing.

Scientific Investigations Report

Hydrology and water quality of the Great Dismal Swamp, Virginia and North Carolina, and implications for hydrologic-management goals and strategies

The Great Dismal Swamp is a peat wetland in the Coastal Plain of southeastern Virginia and northeastern North Carolina. Timber harvesting and the construction of ditches to drain the swamp and facilitate the harvesting are collectively implicated in changes that altered the wetland forests, caused subsidence and decomposition of the peat, and increased the risk of fire. In response to these changes, managers have implemented strategies to control water levels and rewet the swamp using a network of 64 adjustable-height, water-control structures on the ditches. Rewetting the swamp is intended to re-establish the original wetland-forest types, reduce the risk of fire, reduce subsidence and decomposition of the peat, enhance peat accretion, and reduce the risk of fire. Knowledge of responses of the swamp to hydrologic controls, however, is critical to developing and implementing effective management goals and strategies. Because the 2008 South One fire reemphasized the need for this knowledge, the U.S. Geological Survey in cooperation with the U.S. Fish and Wildlife Service began studies in 2009 to identify critical hydrologic controls and responses to these controls. These studies identified water sources, topography, the two-layered hydraulic characteristics of the peat, the absence of peat in some areas, the ditch and road network, water-control structures on the ditches, the Dismal Swamp Canal and associated infrastructure, and wetland forests as the primary hydrologic controls. Precipitation is the only water source across much of the swamp. The eastward flow of streams and groundwater from the Isle of Wight Plain, across the Suffolk scarp, and into the swamp are additional water sources to the western part of the swamp. Vertical differences in the hydraulic characteristics of the peat reflect an upper peat having a high hydraulic conductivity and specific yield overlying a lower peat and sand having lower hydraulic conductivity and specific yield. The upper peat forms the main aquifer for the storage, flow, and release of water from the swamp. Maintaining water in the upper peat is critical to water availability to the wetland forests because of these properties. Groundwater flows from the swamp into the ditches and the Dismal Swamp Canal where it discharges into nearby streams. Discharge typically is to the closest ditch except where a spoil-pile road that impedes flow intervenes between the swamp and the ditch. When groundwater levels in a ditch are about 2 feet lower than levels in the other three ditches surrounding a part of the swamp, however, most groundwater typically discharges to the ditch having the lower level. This occurs even if a spoil-pile road intervenes between the swamp and the ditch having the lower level. Flow to a single ditch shifts watershed boundaries and groundwater divides toward the ditches having higher water levels and demonstrates how flow and discharge are controlled by ditch water levels. Consequently, managing water levels based on these and other hydrologic controls and responses is critical to achieving management objectives. The chemistry of water across the swamp shows the effects of the peat. Dissolved organic carbon concentrations in the groundwater are among the highest reported globally, ranging from 55 to 195 milligrams per liter. The pH of groundwater and ditch water is commonly less than 4.0 standard units because of organic acids. A relation between the pH and specific conductance of groundwater and ditch water reflects water sources, flow paths, and the chemical evolution, as waters from the different sources mix and flow along the paths.

North Carolina, Virginia

Sediment transport, particle size, and loads in North Fish Creek in Bayfield County, Wisconsin, water years 1990-91

North Fish Creek is underused as a trout and salmon hatchery despite its excellent water quality. The shifting-sand streambed in the lower 9 miles of the stream inhibits successful spawning and is a poor habitat for macroinvertebrates, the primary food for juvenile trout and salmon. To provide data necessary for evaluation of potential sand-loading-control practices, the U.S. Geological Survey determined total-sediment transport, particle size, and loads for three sites, designated A, B, and C, on North Fish Creek during water years 1990-91. At site C, the most upstream site, all sediment was transported as suspended sediment. The average annual total-sediment load during 1990- 91 was 479 tons. About 88 percent of the load was transported during periods of snowmelt or storm runoff. About 75 percent of the sediment load was silt- and clay-size particles; the remainder was sand. Total-sediment discharge was calculated by the modified-Einstein procedure to determine total sediment transport-rate relations for site A, the most downstream site, and for site B. Annual totalsediment load was 11,960 tons in water year 1990 and 18,430 tons in water year 1991 at site B. About 97 percent of the total load was transported during periods of snowmelt and storm runoff. About 60 percent of the total-sediment load was sand-size particles. Annual total-sediment loads were 20,690 tons and 33,100 tons in water years 1990 and 1991, respectively, at site A. About 67 percent of the total-sediment load was sand-size particles. Annual average streamflow, as indicated by flow in the Bois Brule River, was about 16 percent below average in water year 1990, and about 4 percent above average in water year 1991. There was little relation between watershed area and sediment loads for the three sites. The watershed of site C is about 41 percent of that of site A, but the sand load at site C was only 1 percent of that at site A. The watershed area between sites B and C is 40 percent of that above site A, but this area yielded 49 percent of the sand load at site A. Nineteen percent of the watershed above site A is between sites A and B, yet this area yielded about 50 percent of the sand load at site A.

Wisconsin

Mercury in wetlands at the Glacial Ridge National Wildlife Refuge, northwestern Minnesota, 2007-9

The Glacial Ridge National Wildlife Refuge was established in 2004 on land in northwestern Minnesota that had previously undergone extensive wetland and prairie restorations. About 7,000 acres of drained wetlands were restored to their original hydrologic function and aquatic ecosystem. During 2007–9, the U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service and the Red Lake Watershed District, analyzed mercury concentrations in wetland water and sediment to evaluate the effect of wetland restoration on mercury methylation. The wetland waters sampled generally were of the calcium/magnesium bicarbonate type. Nitrogen in water was mostly in the form of dissolved-organic nitrogen, with very low dissolved-nitrate and dissolved-ammonia concentrations. About 71 percent of all phosphorus in water was dissolved, with one-half of that in the form of orthophosphorus. Wetland water had total-mercury and methylmercury concentrations ranging from 1.5 to 20 nanograms per liter (ng/L) and 0.2 to 16 ng/L, respectively. Median concentrations were 7.1 and 2.9 ng/L, respectively. About one-half of the mercury in wetland water samples was in the form of methylmercury, but this form ranged from 7 to 81 percent of each sample. Compared to concentrations in stream sediment samples collected throughout the United States, Glacial Ridge National Wildlife Refuge wetland sediment samples contained typical total-mercury concentrations, but methylmercury concentrations were nearly twice as high. The maximum concentration measured in Glacial Ridge National Wildlife Refuge wetland water approached the highest published water methylmercury concentration in uncontaminated waters of which we are aware. However, the upper quartile of water methylmercury concentrations is similar to concentrations reported for some impoundments and wetlands in northwestern Minnesota and North Dakota. Methylmercury concentrations in sampled wetlands were much higher than those from typical lakes or flowing streams throughout the United States. The high concentrations of methylmercury measured in sampled wetlands indicate the potential for substantial methylmercury concentrations in aquatic biota and wildlife that consume those biota. These wetlands also are a methylmercury source for downstream lakes and rivers. The high concentrations of methylmercury in water, its bioaccumulation potential, and its known toxicity in aquatic birds and food webs highlight a need to assess methylmercury in the biota within these ecosystems. Better understanding of factors that control methylmercury production concentrations within aquatic food webs in ecosystems of the Glacial Ridge National Wildlife Refuge would enable resource managers to better understand and manage risk to wildlife.

Minnesota

Geomorphic, flood, and groundwater-flow characteristics of Bayfield Peninsula streams, Wisconsin, and implications for brook-trout habitat

In 2002–03, the U.S. Geological Survey conducted a study of the geomorphic, flood, and groundwater-flow characteristics of five Bayfield Peninsula streams, Wisconsin (Cranberry River, Bark River, Raspberry River, Sioux River, and Whittlesey Creek) to determine the physical limitations for brook-trout habitat. The goals of the study were threefold: (1) to describe geomorphic characteristics and processes, (2) to determine how land-cover characteristics affect flood peaks, and (3) to determine how regional groundwater flow patterns affect base flow. The geomorphic characterization consisted of analyses of historical aerial photographs and General Land Office Survey notes, observations from helicopter video footage, surveys of valley cross sections, and coring. Sources of sediment were identified from the helicopter video and field surveys, and past erosion-control techniques were evaluated. Geomorphic processes, such as runoff sediment erosion, transport, and deposition, are driven by channel location within the drainage network, texture of glacial deposits, and proximity to postglacial lake shorelines; these processes have historically increased because of decreases in upland forest cover and channel roughness. Sources of sediment for all studied streams mainly came from bank, terrace, or bluff erosion along main stem reaches and along feeder tributaries that bisect main-stem entrenched valley sides. Bluff, terrace, and bank erosion were the major sources of sediment to Whittlesey Creek and the Sioux River. No active bluff erosion was observed on the Cranberry River or the Bark River but anecdotal information suggests that landslides occasionally happen on the Cranberry River. For the Bark River, sources of sediment were somewhat evenly divided among road crossings (bridges, culverts, and unimproved forest lanes), terrace erosion, bank erosion, and incision along upper main stems and feeder channels along valley sides. Evaluation of past erosion-control techniques indicated that bluffs were stabilized by a combination of artificial hardening and bioengineering of the bluff base and reducing mass wasting of the tops of the bluffs. Flood hydrographs for the Cranberry River were simulated for four land-cover scenarios—late 20th century (1992–93), presettlement (before 1870), peak agriculture (1928), and developed (25 percent urban). Results were compared to previous simulations of flood peaks for Whittlesey Creek and for North Fish Creek (southern adjacent basin to Whittlesey Creek). Even though most uplands are presently forested, flood peaks simulated for 1992–93 were 1.5 to 2 times larger than presettlement flood peaks. The increased flood peaks caused (1) increased incision along upper main stems and tributaries that bisect entrenched valley sides, (2) bluff and terrace erosion along reaches with entrenched valleys, (3) overbank deposition and bar formation in middle and lower main stems, and (4) aggradation in mouth areas. A base-flow survey was conducted and a groundwater-flow model was developed for the Bayfield Peninsula to delineate groundwater contributing areas. A deep aquifer system, which includes thick deposits of sand and the upper part of the bedrock, is recharged through the permeable sands in the center of the peninsula. Base flow is unevenly distributed among the Bayfield streams and depends on the amount of channel incision and the proximity of the channels to the recharge area and coarse outwash deposits. Groundwater contributing areas for the five streams do not coincide with surface-water-contributing areas. About 89 percent of total recharge to the deep aquifer system discharges to Bayfield streams; the remaining 11 percent directly discharges to Lake Superior. Historical land-cover changes have had negligible effects on groundwater-flow from the deep aquifer system. Available brook-trout habitat is dependent on the locations of groundwater upwellings, the sizes of flood peaks, and sediment loads. Management practices that focus on reducing or slowing runoff from upland areas and increasing channel roughness have potential to reduce flood peaks, erosion, and sedimentation and improve brook-trout habitat in all Bayfield Peninsula streams.

Wisconsin

San Diego Multiple Species Conservation Program (MSCP) Rare Plant Monitoring Review and Revision

Introduction The San Diego Multiple Species Conservation Program (MSCP) was developed for the conservation of plants and animals in the south part of San Diego County, under the California Natural Community Conservation Planning Act of 1991 (California Department of Fish and Game) and the Federal Endangered Species Act of 1973, as amended (16 U.S. Code 1531-1544.) The Program is on the leading edge of conservation, as it seeks to both guide development and conserve at-risk species with the oversight of both State and Federal agencies. Lands were identified for inclusion in the MSCP based on their value as habitat for at-risk plants or plant communities (Natural Community Conservation Planning, 2005). Since its inception in the mid-1990s the Program has protected over 100,000 acres, involving 15 jurisdictions and the U.S. Fish and Wildlife Service (USFWS) and California Department of Fish and Game (CDFG) in the conservation of 87 taxa. Surveys for covered species have been conducted, and management and monitoring have been implemented at some high priority sites. Each jurisdiction or agency manages and monitors their conservation areas independently, while collaborating regionally for long-term protection. The San Diego MSCP is on the forefront of conservation, in one of the most rapidly growing urban areas of the country. The planning effort that developed the MSCP was state-of-the-art, using expert knowledge, spatial habitat modeling, and principles of preserve design to identify and prioritize areas for protection. Land acquisition and protection are ahead of schedule for most jurisdictions. Surveys have verified the locations of many rare plant populations known from earlier collections, and they provide general information on population size and health useful for further conservation planning. Management plans have been written or are in development for most MSCP parcels under jurisdictional control. Several agencies are developing databases for implementation and management tracking. In many ways this program is at the cutting edge of regional conservation, testing concepts, developing techniques, and demonstrating conservation effectiveness in new and uncharted ways. Periodic program review is crucial to the continued success of the program, as it moves from a phase of planning and acquisition to one of management and monitoring. Ecological monitoring is the key to assessing the success of the protection and management implemented at each individual reserve and for the MSCP as a whole. The ultimate goal of the Program is conservation of at-risk taxa and their habitats, as well as underlying ecological processes that contribute to sustainability of the ecosystem. Monitoring guidelines and timetables were developed by Ogden Environmental and Energy Services Co., Inc. (1996), and reviewed by Conservation Biology Institute (2001). The Program is in transition now, from the initial stage of land protection to one of land management and monitoring to determine population responses to management regimes. Several agencies have already invested substantial effort in status and trend monitoring, while others are developing their monitoring plans. Management is ongoing at several sites. With both management and monitoring, collaboration and coordination among jurisdictions can be especially fruitful in conserving resources and maximizing success.

Scientific Investigations Report

Potential effects of energy development on environmental resources of the Williston Basin in Montana, North Dakota, and South Dakota—Species of conservation concern

The ecosystems of the Williston Basin provide direct and indirect benefits to society. These benefits include carbon sequestration, flood control, nutrient rich soils for agricultural productivity, and habitat for wildlife. This chapter’s main focus is on the effects of energy development on species that occupy the ecosystems in the Williston Basin. We compiled a list of documented species of conservation concern that are of most interest to Federal regulators and resource managers. Species of concern were either listed as endangered or threatened under the Endangered Species Act or listed by States as species of concern in Natural Heritage Program checklists or State Wildlife Action Plans. All told, we determined that 357 species of concern likely occupy the Williston Basin. These species represented seven different taxonomic groups: plants (native and nonnative), terrestrial invertebrates, birds, mammals, reptiles and amphibians, and fish and mussels. We reviewed the existing scientific information pertaining to potential effects of energy development on these taxonomic groups. Currently, little is known about the abundance and distribution of many of these species. But some information exists that may be useful in predicting the potential effects of energy development on certain taxonomic groups. Most of this information has been developed through scientific research focused on effects to mammal and bird populations. Effects to other taxonomic groups seems to be understudied. In general, it seems that disturbances and modifications associated with development have the potential to negatively affect a wide range of species; however, many studies produce uncertain results because they are not designed to compare populations before and after energy development takes place. Most of these studies also do not monitor resources over multiple years and thus cannot detect population trends. Likewise, there are few examples of landscape-scale assessments of the cumulative effects of energy development that could be used for species or habitat management purposes. We suggest that more research needs to be completed to measure potential effects to a broad range of species in multiple taxonomic groups. This may require also developing some understanding about the basic ecology of many of the species covered in this report. In concert with this more basic research, we also suggest that more comprehensive assessments of potential negative cumulative effects across the Williston Basin should be developed in an effort to guide more strategic management of biological resources.

Montana, North Dakota, South Dakota

Davis Pond freshwater prediversion biomonitoring study: freshwater fisheries and eagles

In January 2001, the construction of the Davis Pond freshwater diversion structure was completed by the U.S. Army Corps of Engineers. The diversion of freshwater from the Mississippi River is intended to mitigate saltwater intrusion from the Gulf of Mexico and to lessen the concomitant loss of wetland areas. In addition to the freshwater inflow, Barataria Bay basin would receive nutrients, increased flows of sediments, and water-borne and sediment-bound compounds. The purpose of this biomonitoring study was, therefore, to serve as a baseline for prediversion concentrations of selected contaminants in bald eagle ( Haliaeetus leucocephalus ) nestlings (hereafter referred to as eaglets), representative freshwater fish, and bivalves. Samples were collected from January through June 2001. Two similarly designed postdiversion studies, as described in the biological monitoring program, are planned. Active bald eagle nests targeted for sampling eaglet blood (n = 6) were generally located southwest and south of the diversion structure. The designated sites for aquatic animal sampling were at Lake Salvador, at Lake Cataouatche, at Bayou Couba, and along the Mississippi River. Aquatic animals representative of eagle prey were collected. Fish were from three different trophic levels and have varying feeding strategies and life histories. These included herbivorous striped mullet ( Mugil cephalus ), omnivorous blue catfish ( Ictalurus furcatus ), and carnivorous largemouth bass ( Micropterus salmoides ). Three individuals per species were collected at each of the four sampling sites. Freshwater Atlantic rangia clams ( Rangia cuneata ) were collected at the downstream marsh sites, and zebra mussels ( Dreissena spp.) were collected on the Mississippi River. The U.S. Geological Survey (USGS) Biomonitoring of Environmental Status and Trends (BEST) protocols served as guides for fish sampling and health assessments. Fish are useful for monitoring aquatic ecosystems because they accumulate pesticides and other contaminants. Biomarker data on individual fish, generated at the USGS National Wetlands Research Center (Lafayette, La.), included percent white blood cells in whole blood, spleen weight to body weight ratio, liver weight to body weight ratio, condition factor, splenic macrophage aggregates, and liver microsomal 7-ethoxyresorufin-o-deethylase (EROD) activity. Fish age was estimated by comparing total lengths with values from the same species in the Southeast United States as determined from the literature. Contaminant analyses were coordinated by the U.S. Fish and Wildlife Service (USFWS) Analytical Control Facility (Laurel, Md.), where residues of organochlorine (OC) pesticides, total polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), aliphatic hydrocarbons (AHs), and trace elements were determined. The organic contaminant data were generated at the Mississippi State University Chemical Lab (Mississippi State, Miss.), and the inorganic contaminant data were generated by the Texas A&M University Geochemical and Environmental Research Group (College Station, Tex.). Statistical tests were performed to assess relationships among contaminants, fish age, fish species, and collection sites. Trends in interspecific differences among fish in concentrations of contaminants were noted. Striped mullet (hereafter mullet) frequently displayed the highest chemical concentrations. Levels of contaminants were generally higher in samples obtained from the Mississippi River than in those collected from the diversion area and were higher in mussels and clams (hereafter bivalves) than in fish. Because the Mississippi River sampling site for mullet and largemouth bass was downriver of the structure and south of New Orleans and the catfish site was upriver, the downriver data may not be directly reflective of the results from the receiving waters at the Davis Pond structure. Compared to the Caernarvon freshwater prediversion study in 1990 that assessed possible influx of contaminants with the freshwater diversion, contaminant levels in fishes and bivalves in this study were generally lower, yet three nontoxic inorganic elements in Davis Pond fish samples exhibited ranges of concentrations that were more than two times higher than did those from Caernarvon. Levels in bivalves were different between diversions but about equal in the numbers of trace elements showing high levels per location. Contaminant values were compared to those listed in various literature and agency sources, both regional and national, including the National Contaminant Biomonitoring Program (NCBP), in which the 85th percentile and above represents what is considered to be an elevated contaminant concentration and cause for concern. Generally, bivalves were at the high end of their ranges for both organic and inorganic contaminants. In this study, OCs were detectable in 67 percent of fish from the Mississippi River site, ranging from 0.15 to 1.09 μg/g wet weight (ww) or fresh weight (fw), and in 11 percent of the fish from the marsh sites, ranging from 0.06 to 0.612 μg/g ww. Bivalves from the Mississippi River had OC levels of 0.096 μg/g ww, whereas none were detectable in bivalves at the marsh sites. In this study, p,p ’-dichlorodiphenyldichloroethylene ( p,p’ -DDE) (a biodegradation product of DDT [dichlorodiphenyl trichloroethane]) and total PCBs were the most frequently detected OCs and were primarily from the Mississippi River. For total OC content, using adjusted least squares means, some significant interactions were noted between fish species and sites. PAHs were detected in aquatic animals at all sites (range of 0.017–17.534 μg/g ww), as were AHs (range of 0.423–4.549 μg/g ww); the highest levels of PAHs and AHs were found in bivalves from the Mississippi River. When analysis of variance (α = 0.05) was performed with data from aquatic animals, there were only two significant relationships between PAHs, AHs, and OCs between species, site, and age or the interaction among these variables. There was an interaction between fish species and n-decane (an AH) in that mullet and largemouth bass had significantly higher levels than did catfish ( P = 0.0175). When general linear means were used to investigate associations of inorganic contaminants among fish species, site, and age or any interactions among these variables, no significant results were noted for arsenic, cadmium, lead, beryllium, boron, molybdenum, or nickel. The range of mercury in fish in this study was 0.04–0.14 μg/g ww (0.14– 0.48 μg/g dry weight [dw]), with the most elevated levels detected in predatory largemouth bass at the sampling point farthest downstream from the structure and within the marsh area. Mercury was positively correlated with fish age ( P = 0.0152), where levels were estimated to increase 0.0253 parts per million (ppm) dw per year. In the Mississippi River, catfish showed significantly higher levels of mercury than did mullet or largemouth bass ( P = 0.00167). Among fish species, mullet displayed the highest levels in fish of aluminum, barium, manganese, and iron, all considered to have low toxicity in hydrologic systems. An interaction between fish and site was seen with aluminum ( P = 0.0031), where concentrations in mullet were significantly higher in the Mississippi River than at the other sites, as was also seen with barium ( P = 0.0009), chromium ( P = <0.0001), manganese ( P = 0.0004), strontium ( P = 0.0074), vanadium ( P = 0.0156), and zinc ( P = 0.0059). For iron ( P = 0.0.0001), mullet and largemouth bass at both the Mississippi River and Lake Salvador showed higher levels than did catfish, and these two species showed higher levels at two of the four sites. An interaction between fish and site was also seen with chromium ( P = <0.0001) in that concentrations in mullet were significantly higher in the Mississippi River than at the other sites, as was also seen with strontium ( P = 0.0074), vanadium ( P = 0.0156), and zinc ( P = 0.0059), metals for which deleterious effects have been demonstrated in other ecosystems. The NCBP program lists the 85th percentile for zinc at 34.2 μg/g fw (117.9 μg/g dw). In the Davis Pond prediversion biomonitoring study (hereafter the current study), one fish (MUL31RIVER, fish ID 8) showed values higher than that (125.4 μg/g dw or 37.54 μg/g ww), and the Mississippi River bivalve sample (MUSSRIVER) had a value of 140 μg/g dw (41.2 μg/g ww). In the current study, approximately 86 percent of the fish had measurable selenium levels, yet none reached the 85th percentile. The 85th percentile for selenium from the NCBP was 0.73 μg/g ww. Significantly higher levels of selenium were seen in mullet than in largemouth bass and catfish ( P = 0.0023). The NCBP 85th percentile for lead is 0.22 μg/g ww (0.76 μg/g dw). In the current study, the range of concentrations of lead was as much as 18.3 ppm dw (MUL31RIVER, fish ID 8), with the three most elevated values (range of 3.46–5.31 μg/g ww) coming from mullet from the Mississippi River. Biomarker data are measurable and directly reflect the condition of the animal, and measuring more than one biomarker in an individual increases confidence in health assessments. In the current study, biomarkers included macrophage aggregates (MAs), liver (hepatosomatic index [HSI]) and spleen (splenosomatic index [SSI]) weight to body weight ratios, percent white blood cells (WBCs) in whole blood, and condition factor. Few significant differences were noted with any of the biomarkers between sites, and there were no relationships between species and sites. For improved use of biomarker assessments, an increase in fish sample size would be useful for postdiversion sampling, as would comparisons of fish of the same sex and reproductive condition. During the current study, success for eagle nests in the diversion area and reference sites was similar as determined by numbers of nestlings fledged. When temperatures were below average during winter 2000, nests in both regions similarly failed. At each nest, the primary evidence of food items was small mammals. Eaglets (n = 6) generally appeared healthy, and whole blood concentrations of organic contaminants exceeded detection limits with three incidences of p,p’ -DDE (0.002–0.006 μg/L ww) and one incidence of oxychlordane (0.002 μg/L ww). The levels of p,p’ -DDE were well below those that have been inversely correlated with productivity and success rates of nesting bald eagles on a regional scale. The low values found in the whole blood samples for OC pesticides and PCBs were even lower when corrected for plasma volume. Aluminum values were 3.66 and 5.75 μg/L in two samples, zinc ranged from 5.21 to 6.77 μg/L ww in six samples, and silicon ranged from 1.7 to 4.6 μg/L in four samples. Selenium was detectable in each bird with the range at 0.332–0.566 μg/L ww, and strontium ranged from 0.0581 to 0.0975 μg/L ww. Mercury was detectable in blood samples from each bird and ranged from 0.0254 to 0.0845 μg/L ww, whereas lead was detectable in four samples and ranged from 0.0042 to 0.0136 μg/L ww. Although no detectable levels of total PCBs were found (also correlated with decreased reproductive productivity), 70 percent of the aquatic animals from the Mississippi River contained total PCBs (range 0.13–0.79 μg/L), whereas only about 7 percent of the aquatic animals sampled from the marsh area contained PCBs. Suggestions for postdiversion sampling include lowering the analytical detection limit for some metals, sampling aquatic animals over the course of a single season, obtaining a higher sample number of mature fish of one species (for example, blue catfish) within a range of total lengths for biomarker analyses, obtaining otoliths for estimating fish ages, assessing dioxins in eaglet blood, examining triazines in water, and obtaining all Mississippi River fish samples as close to the Davis Pond structure intake as possible. Because contaminants found in blood of eaglets reflect their prey species and because of the contaminant levels found in fish in the current study, eaglets may not be consuming primarily these species; therefore, obtaining juvenile nutria ( Myocastor coypus ) or turtle species for contaminant analyses might be considered, as well as collecting greater blood volume and using plasma to measure OCs and PCBs. Data obtained postdiversion will be compared with prediversion data to monitor changes.

Louisiana

Evaluation of restoration alternatives using water-budget tools for the Wapato Lake National Wildlife Refuge, northwestern Oregon

The lakebed in Wapato Lake National Wildlife Refuge (NWR) in northwestern Oregon was farmed for decades prior to the establishment of the refuge in 2013. Planning for restoration of these lands required extensive data collection and construction of a water budget and tools to design and evaluate potential restoration strategies. The U.S. Geological Survey (USGS) and U.S. Fish and Wildlife Service worked together to monitor streamflow and water levels in and around Wapato Lake NWR, apply the USGS Shoreline Management Tool (SMT), then construct and apply a water-budget-based Water Management Scenario Tool (WMST). The SMT was used to determine the spatial availability of different water depths (as potential habitat for different species) as a function of water level and other factors, based on topographic data. The WMST uses a water-budget approach to predict daily water levels, inflows, outflows, and areas of specific categories of water depth in the refuge over the course of a water year in response to a range of hydrologic and meteorological conditions and potential water-management strategies. In this study, two hypothetical water-management strategies were simulated to predict their effect on water levels and areas with specific water depths as an indicator of potential habitat. In the first scenario, several tributaries that had been diverted around the lakebed since the 1930s were reconnected to the lake, and an outflow weir was used to control lake level and to create a lake and seasonal wetlands of specific depths. In the second scenario, an outflow weir was combined with pumps to help meet target lake levels. Results showed that reconnecting the largest three tributaries to Wapato Lake would provide sufficient water to create a range of aquatic conditions in most years. For a median water year, rainfall and tributary flows in these scenarios provided 99 percent of total inputs to the lake, whereas pumping, weir outflows, and open-water evaporation accounted for 95–97 percent of losses. Management of lake levels could be accomplished with a variable-elevation outflow weir or a combination of a weir and pumps. The lake would take longer to fill to a higher seasonal target level during a dry year. Without an outflow weir or other means of allowing water to flow out of the lake, the largest of two existing pumps would need to be used during late spring or early summer to attain a lower seasonal target water level in summer. High-water conditions downstream of Wapato Lake may prevent the use of a simple outflow weir, as historical downstream water levels in winter and spring sometimes were higher than the target water levels used in these scenarios. Water-budget-based methods applied in this study have proven to be valuable for the design and evaluation of potential restoration strategies at Wapato Lake NWR.

Wapato Lake National Wildlife Refuge

Geomorphic responses of Duluth-area streams to the June 2012 flood, Minnesota

In 2013, the U.S. Geological Survey, in cooperation with the Minnesota Pollution Control Agency, completed a geomorphic assessment of 51 Duluth-area stream sites in 20 basins to describe and document the stream geomorphic changes associated with the June 2012 flood. Heavy rainfall caused flood peaks with annual exceedance probabilities of less than 0.002 (flood recurrence interval of greater than 500 years) on large and small streams in and surrounding the Duluth area. A geomorphic segment-scale classification previously developed in 2003–4 by the U.S. Geological Survey for Duluth-area streams was used as a framework to characterize the observed flood-related responses along a longitudinal continuum from headwaters to rivermouths at Lake Superior related to drainage network position, slope, geologic setting, and valley type. Field assessments in 2013 followed and expanded on techniques used in 2003–4 at intensive and rapid sites. A third level of assessment was added in 2013 to increase the amount of quantitative data at a subset of 2003–4 rapid sites. Characteristics of channel morphology, channel bed substrate, exposed bars and soft sediment deposition, large wood, pools, and bank erosion were measured; and repeat photographs were taken. Additional measurements in 2013 included identification of Rosgen Level II stream types. The comparative analyses of field data collected in 2003–4 and again in 2013 indicated notable geomorphic changes, some of them expected and others not. As expected, in headwaters with gently sloping wetland segments, geomorphic changes were negligible (little measured or observed change). Downstream, middle main stems generally had bank and bluff erosion and bar formation as expected. Steep bedrock sites along middle and lower main stems had localized bank and bluff erosion in short sections with intermittent bedrock. Lower main stem and alluvial sites had bank erosion, widening, gravel bar deposition, and aggradation. Bar formation and accumulation of gravel was more widespread than expected among all main stems, especially for sites upstream and downstream from channel constrictions from road crossings, or even steep sites with localized, more gently sloping sections. Decreases in large wood and pools also were observed throughout the longitudinal continuum of main-stem sites, with immediate implications for fish and benthic invertebrate aquatic habitat. Whether or not the geomorphic conditions will return to their preflood condition depends on the location along the longitudinal continuum. The amount of large wood and pools may return after more moderate floods, whereas bars with coarse material may remain in place, locally altering flow direction and causing continued bank erosion. Results from this study can be used by local managers in postflood reconstruction efforts and provide baseline information for continued monitoring of geomorphic responses to the June 2012 flood.

Minnesota

Analysis and simulation of water-level, specific conductance, and total phosphorus dynamics of the Loxahatchee National Wildlife Refuge, Florida, 1995-2006

The Arthur R. Marshall Loxahatchee Wildlife Refuge (Refuge) was established in 1951 through a license agreement between the South Florida Water Management District and the U.S. Fish and Wildlife Service (USFWS) as part of the Migratory Bird Conservation Act. Under the license agreement, the State of Florida owns the land of the Refuge and the USFWS manages the land. Fifty-seven miles of levees and borrow canals surround the Refuge. Water in the canals surrounding the marsh is controlled by inflows and outflows through control structures. The transport of canal water with higher specific conductance and nutrient concentrations to the interior marsh has the potential to alter critical ecosystem functions of the marsh. Data-mining techniques were applied to 12 years (1995-2006) of historical data to systematically synthesize and analyze the dataset to enhance the understanding of the hydrology and water quality of the Refuge. From the analysis, empirical models, including artificial neural network (ANN) models, were developed to answer critical questions related to the relative effects of controlled releases, precipitation, and meteorological forcing on water levels, specific conductance, and phosphorous concentrations of the interior marsh. Data mining is a powerful tool for converting large databases into information to solve complex problems resulting from large numbers of explanatory variables or poorly understood process physics. For the application of the linear regression and ANN models to the Refuge, data-mining methods were applied to maximize the information content in the raw data. Signal processing techniques used in the data analysis and model development included signal decomposition, digital filtering, time derivatives, time delays, and running averages. Inputs to the empirical models included time series, or signals, of inflows and outflows from the control structures, precipitation, and evapotranspiration. For a complex hydrologic system like the Refuge, the statistical accuracy of the models and predictive capability were good. The water-level models have coefficient of determination (R 2 values ranging from 0.90 to 0.98. The R 2 for the specific conductance model is 0.82, and the R2 for the total phosphorus model is 0.51. The accuracy of the models was attributable to the quantity and quality of the available data. To make the models directly available to all stakeholders, an easy-to-use decision support system (DSS) called the Loxahatchee Artificial Neural Network Model (LOXANN) DSS was developed as a spreadsheet application that integrates the historical database, linear regression and ANN models, model controls, streaming graphics, and model output. The LOXANN DSS allows Refuge managers and other users to easily execute the water level, specific conductance, and phosphorous models to evaluate various water-resource management scenarios. The user is able to choose from three options in setting the control-structure flows: as a percentage of historical flow, as a constant flow, or as a user-defined hydrograph. Output from the LOXANN DSS includes tabular time series of predictions of the measured data and predictions of the user-specified conditions. A three-dimensional visualization routine also was developed that displays longitudinal specific conductance conditions. Two scenarios were simulated with the LOXANN DSS. One scenario increased the historical flows at four control structures by 40 percent. The second scenario used a user-defined hydrograph to set the outflow from the Refuge to the weekly average inflow to the Refuge delayed by 2 days. Both scenarios decreased the potential of canal water intruding into the marsh by decreasing the slope of the water level between the canals and the marsh.

Scientific Investigations Report

Spatial stream network modeling of water temperature within the White River Basin, Mount Rainier National Park, Washington

Water temperature is a primary control on the occurrence and distribution of fish and other ectothermic aquatic species. In the Pacific Northwest, cold-water species such as Pacific salmon ( Oncorhynchus spp.) and bull trout ( Salvelinus confluentus ) have specific temperature requirements during different life stages that must be met to ensure the viability of their populations. Rivers draining Mount Rainier in western Washington, including the White River along its northern flank, support a number of cold-water fish populations, but the spatial distribution of water temperatures, particularly during late-summer baseflow during August and September, and the climatic, hydrologic, and physical processes regulating it are not well constrained. Spatial stream network (SSN) models, which are generalized linear models that incorporate streamwise spatial autocovariance structures, were fit to mean and 7-day average daily maximum water temperature for August and September for the White River Basin. The SSN models were calibrated using water temperature measurements collected in 2010 through 2020. The extent of the models included the White River and its tributaries upstream from its confluence with Silver Creek in Mount Rainier National Park, Washington. SSN models incorporated covariates hypothesized to represent the climatic, hydrologic, and physical processes that influence water temperature. SSN models were fit to the measured data and compared to generalized linear models that lacked spatial autocovariance structures. Statistically significant covariates within the best-fit models included the proportion of ice cover and forest cover within the basin, mean August air temperature, the proportion of consolidated geologic units, and snow-water equivalent. Statistical models that included spatial autocovariance structures had better predictive performance than those that did not. Additionally, models of mean August and September water temperature had better predictive performance than those of 7-day average daily maximum temperature in August and September. Predictions of the spatial distribution of water temperature were similar between August and September with a general warming in the downstream part of the mainstem White River compared to cooler water temperatures in the high-elevation headwater streams. The proportion of ice cover emerged as an inversely related significant covariate to both mean August and September water temperature because streams that receive glacial meltwater are colder than non-glaciated streams. Water temperatures of the upper White River increased downstream and are attributed to warming of water temperature from accumulated solar radiation and inflow of non-glaciated tributaries. Estimated water temperatures for the upper White River model are 3–4 degrees Celsius (°C) warmer for tributaries, but 1–2 °C cooler for the mainstem compared to the regional-scale model. Differences between the upper White River SSN model and the regional-scale NorWeST model are attributed to the fact that the upper White River SSN included water temperature observations specific to the upper White River, whereas water temperature observations from lower elevation streams and downstream from the Mount Rainer National Park boundary were used in the regional scale model.

Washington

Evaluation of alternative groundwater-management strategies for the Bureau of Reclamation Klamath Project, Oregon and California

The water resources of the upper Klamath Basin, in southern Oregon and northern California, are managed to achieve various complex and interconnected purposes. Since 2001, irrigators in the Bureau of Reclamation Klamath Irrigation Project (Project) have been required to limit surface-water diversions to protect habitat for endangered freshwater and anadromous fishes. The reductions in irrigation diversions have led to an increased demand for groundwater by Project irrigators, particularly in drought years. The potential effects of sustained pumping on groundwater and surface-water resources have caused concern among Federal and state agencies, Indian tribes, wildlife groups, and groundwater users. To aid in the development of a viable groundwater-management strategy for the Project, the U.S. Geological Survey, in collaboration with the Klamath Water and Power Agency and the Oregon Water Resources Department, developed a groundwater-management model that links groundwater simulation with techniques of constrained optimization. The overall goal of the groundwater-management model is to determine the patterns of groundwater pumping that, to the extent possible, meet the supplemental groundwater demands of the Project. To ensure that groundwater development does not adversely affect groundwater and surface-water resources, the groundwater-management model includes constraints to (1) limit the effects of groundwater withdrawal on groundwater discharge to streams and lakes that support critical habitat for fish listed under the Endangered Species Act, (2) ensure that drawdowns do not exceed limits allowed by Oregon water law, and (3) ensure that groundwater withdrawal does not adversely affect agricultural drain flows that supply a substantial portion of water for irrigators and wildlife refuges in downslope areas of the Project. Groundwater-management alternatives were tested and designed within the framework of the Klamath Basin Restoration Agreement (currently [2013] awaiting authorizing Federal legislation), which would establish a permanent limit on the amount of surface water that can be diverted annually to the Project. Groundwater-management scenarios were evaluated for the period 1970•2004; supplemental groundwater demand by the Project was estimated as the part of irrigation demand that would not have been satisfied by the surface-water diversion allowed under the Klamath Basin Restoration Agreement. Over the 35-year management period, 22 years have supplemental groundwater demand, which ranges from a few thousand acre-feet (acre-ft) to about 100,000 acre-ft in the driest years. The results of the groundwater-management model indicate that supplemental groundwater pumping by the Project can be managed to avoid adverse effects to groundwater discharge that supports critical aquatic habitat. The existing configuration of wells in the Project would be able to meet groundwater-pumping goals in 14 of the 22 years with supplemental groundwater demand; however, substantial irrigation shortages can be expected during drought periods when the demand for supplemental groundwater is highest. The maximum irrigation-season withdrawal calculated by the groundwater-management model is about 60,000 acre-ft, the average withdrawal in drought years is about 54,000 acre-ft, and the amount of unmet groundwater demand reaches a maximum of about 45,000 acre-ft. A comparison of optimized groundwater withdrawals by geographic region shows that the highest annual withdrawals are associated with wells in the Tule Lake and Klamath Valley regions of the Project. The patterns of groundwater withdrawal also show that a substantial amount of the available pumping capacity is unused due to the restrictions imposed by drawdown constraints. Subsequent model applications were used to evaluate the sensitivity of optimization results to various factors. A sensitivity analysis quantified the changes in optimized groundwater withdrawals that result from changes in drawdown-constraint limits. The analysis showed the potential for substantial increases in withdrawals of groundwater with less restrictive drawdown limits at drawdown-control sites in the California part of the model. Systematic variation of the drains-constraint limit yielded a trade-off curve between optimized groundwater withdrawals and the allowable reduction in groundwater discharge to the Project drain system. Additional model applications were used to assess the value of increasing the pumping capacity of the network of wells serving the Project, and the relation between reduced off-Project groundwater pumping and increased pumping by Project irrigators.

California;Oregon

Water Quality and Biological Characteristics of the Middle Fork of the Saline River, Arkansas, 2003-06

The Middle Fork of the Saline River has many qualities that have been recognized by State and Federal agencies. The Middle Fork provides habitat for several rare aquatic species and is part of a larger stream system (the Upper Saline River) that is known for relatively high levels of species richness and relatively high numbers of species of concern. Water-quality samples were collected and streamflow was measured by the U.S. Geological Survey at three sites in the Middle Fork Basin between October 2003 and October 2006. The Arkansas Department of Environmental Quality collected discrete synoptic water-quality samples from eight sites between January 2004 and October 2006. The Arkansas Department of Environmental Quality also sampled fish (September-October 2003) and benthic macroinvertebrate communities (September 2003-December 2005) at five sites. Streamflow varied annually among the three streamflow sites from October 2003 to October 2006. The mean annual streamflow for Brushy Creek near Jessieville (MFS06) was 0.72 cubic meters per second for water years 2004-2006. The Middle Fork below Jessieville (MFS05) had a mean annual streamflow of 1.11 cubic meters per second for water years 2004-2006. The Middle Fork near Owensville (MFS02), the most downstream site, had a mean annual streamflow of 3.01 cubic meters per second. The greatest streamflows at the three sites generally occurred in the winter and spring and the least in the summer. Nutrient dynamics in the Middle Fork are controlled by activities in the basin and processes that occur in the stream. Point sources and nonpoint sources of nutrients occur in the Middle Fork Basin that could affect the water-quality. Nitrogen and phosphorus concentrations generally were greatest in Mill Creek (MFS04E) and in the Middle Fork immediately downstream from the confluence with Mill Creek (MFS04) with decreasing concentrations at sites farther downstream in Middle Fork. The site in Mill Creek is located downstream from a wastewater-treatment plant discharge and concentrations at sites farther downstream probably had lesser concentrations because of dilution effects and from algal uptake. Nutrient concentrations generally were significantly greater during high-flow conditions compared to base-flow conditions. Flow-weighted nutrient concentrations were computed for the three streamflow sites and were compared to 82 relatively undeveloped sites identified across the Nation, to the Alum Fork of the Saline River near Reform, Arkansas, and to the Illinois River south of Siloam Springs, Arkansas, a site influenced by numerous point and nonpoint sources of nutrients. Annual flow-weighted nutrient concentrations for MFS06, MFS05, and MFS02 were greater than relatively undeveloped sites, but were substantially less than the Illinois River south of Siloam Springs. Fecal indicator bacteria concentrations were slightly greater at MFS06 and MFS05 compared to concentrations at MFS02 for October 2003 to October 2006. MFS05 had the greatest E.coli concentrations and MFS06 had the greatest fecal coliform concentrations. Overall, fecal indicator bacteria concentrations were significantly greater for samples collected during high-flow conditions compared to samples collected during low-flow conditions at all three sites. Suspended-sediment concentrations did not vary significantly among MFS06, MFS05, and MFS02 for all the samples collected from October 2003 to October 2006. Suspended-sediment concentrations were significantly greater in samples collected during high-flow conditions compared to samples collected during base-flow conditions. Synoptic samples indicated varied total suspended-solids distributions from upstream to downstream in the Middle Fork between January 2004 and October 2006. Overall, total suspended-solids values were the greatest at site MFS02 and decreased at sites upstream and downstream. Turbidity measured when water-quality samples were

Scientific Investigations Report

Acute and chronic sensitivity of white sturgeon ( Acipenser transmontanus ) and rainbow trout ( Oncorhynchus mykiss ) to cadmium, copper, lead, or zinc in laboratory water-only exposures

White sturgeon (Acipenser transmontanus) are experiencing poor recruitment in the trans boundary reach of the upper Columbia River in eastern Washington State. Limited toxicity data indicated that early life stages of white sturgeon are sensitive to metals. In acute 4-day (d) exposures with larval white sturgeon, previous studies have reported that the 4-day median lethal concentrations (LC50) based on biotic ligand model (BLM) normalization for copper were below the U.S. Environmental Protection Agency national recommended acute water-quality criterion. In previously published chronic 66-d exposures starting with newly fertilized eggs of white sturgeon, 20-percent lethal effect concentrations (LC20s) for copper, cadmium, or zinc generally were within a factor of two of the chronic values of the most sensitive fish species in the databases of the U.S. Environmental Protection Agency water-quality criteria (WQC) for the three metals. However, there were some uncertainties in the chronic exposures previously performed with white sturgeon, including (1) low control survival (37 percent), (2) more control fish tested in each replicate compared to other treatments, (3) limited replication of treatments (n=2), (4) lack of reported growth data (such as dry weight), and (5) wide dilution factors for exposure concentrations (6- to 8-fold dilutions). The U.S. Environmental Protection Agency concluded that additional studies are needed to generate more toxicity data to better define lethal and sublethal toxicity thresholds for metals for white sturgeon. The objective of the study was to further evaluate the acute and chronic toxicity of cadmium, copper, lead, or zinc to early life stages of white sturgeon in water-only exposures. Toxicity tests also were performed with commonly tested rainbow trout (Oncorhynchus mykiss) under similar test conditions to determine the relative sensitivity between white sturgeon and rainbow trout to these metals. Toxicity data generated from this study were used to evaluate the sensitivity of early life stages of white sturgeon and rainbow trout relative to data published for other test organisms. Toxicity data generated from this study also were used to evaluate the level of protection of U.S. Environmental Protection Agency WQC or Washington State water-quality standards (WQS) for copper, zinc, cadmium, or lead to white sturgeon inhabiting the upper Columbia River. Chapter A of this report summarizes the results of acute toxicity tests performed for 4 d with white sturgeon and rainbow trout exposed to copper, cadmium, or zinc. Chapter B of this report summarizes the results of chronic toxicity tests performed for as many as 53 days with white sturgeon or rainbow trout exposed to copper, cadmium, zinc, or lead. Appendixes to the report are available at http://pubs.usgs.gov/sir/2013/5204 . Supporting documentation for chapter A toxicity testing is provided in appendix 1. Supporting documentation for chapter B toxicity testing is provided in Appendix 2. Supporting documentation on analysis of water chemistry for chapter A and chapter B is provided in appendix 3 and 4. The rationale for applying corrections to measured copper and zinc values in water samples from some of the toxicity tests performed in chapter A is provided in appendix 5. A summary of dissolved organic carbon measurement variability and implications for biotic ligand model normalization for toxicity data summarized in chapter A and chapter B are provided in appendix 6. An evaluation of an interlaboratory comparison of analyses for dissolved organic carbon in water from the U.S. Geological Survey Columbia Environmental Research Center and University of Saskatchewan is provided in appendix 7. Finally, appendix 8 provides a summary of retesting of white sturgeon in 2012 to determine if improved survival of sturgeon would affect copper effect concentrations in 24-d copper exposures started with newly hatched larvae, and to evaluate the effect of light intensity or temperature on the response of newly hatched larvae during a 25-d study.

Scientific Investigations Report

Probability analysis of the relation of salinity to freshwater discharge in the St. Sebastian River, Florida

The St. Sebastian River lies in the southern part of the Indian River basin on the east coast of Florida. Increases in freshwater discharge due to urbanization and changes in land use have reduced salinity in the St. Sebastian River and, consequently, salinity in the Indian River, affecting the commercial fishing industry. Wind, water temperature, tidal flux, freshwater discharge, and downstream salinity all affect salinity in the St. Sebastian River estuary, but freshwater discharge is the only one of these hydrologic factors which might be affected by water-management practices. A probability analysis of salinity conditions in the St. Sebastian River estuary, taking into account the effects of freshwater discharge over a period from May 1992 to March 1996, was used to determine the likelihood (probability) that salinities, as represented by daily mean specific- conductance values, will fall below a given threshold. The effects of freshwater discharge on salinities were evaluated with a simple volumetric model fitted to time series of measured specific conductance, by using nonlinear optimization techniques. Specific-conductance values for two depths at monitored sites represent stratified flow which results from differences in salt concentration between freshwater and saltwater. Layering of freshwater and saltwater is assumed, and the model is applied independently to each layer with the assumption that the water within the layer is well mixed. The model of specific conductance as a function of discharge (a salinity response model) was combined with a model of residual variation to produce a total probability model. Flow distributions and model residuals were integrated to produce a salinity distribution and determine differences in salinity probabilities as a result of changes in water-management practices. Two possible management alternatives were analyzed: stormwater detention (reducing the peak rate of discharge but not reducing the overall flow volume) and stormwater retention (reducing peak discharges without later release). Detention of freshwater discharges increased the probability of specific- conductance values falling below a given limit (20,000 microsiemens per centimeter) for all sites but one. The retention of freshwater input to the system decreased the likelihood of falling below a selected limit of specific conductance at all sites. For limits of specific conductance (1,000 microsiemens per centimeter or 20,000 microsiemens per centimeter, depending on the site), the predicted days of occurrence below a limit decreased ranging from 17 to 68 percent of the predicted days of occurrence for unregulated flow. The primary finding to be drawn from the discharge-salinity analysis is that an empirical-response model alone does not provide adequate information to assess the response of the system to changes in flow regime. Whether a given level of discharge can produce a given response on a given day is not as important as the probability of that response on a given day and over a period of many days. A deterministic model of the St. Sebastian River estuary based only on discharge would predict that retention of discharge peaks should increase the average salinity conditions in the St. Sebastian River estuary. The probabilistic model produces a very different response indicating that salinity can decrease by a power of three as discharges increase, and that random factors can predominate and control salinity until discharges increase sufficiently to flush the entire system of saltwater.

Florida