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At least 379 records · Page 21Linked to original sources

Post-spawning mortality of rainbow trout (Salmo gairdneri) associated with Lactobacillus

A Lactobacillus sp. was consistently isolated from dead and moribund postspawning brood stock rainbow trout. Pathology was characterized by massive chronic inflammation throughout the abdominal cavity and formation of pseudomembranes. The bacteria were most abundant in spent testes. Repeated handling was considered to be the major stressor leading to this infection and subsequent high mortality.

Journal of Wildlife Diseases↗

Effects of pelletized anticoagulant rodenticides on California quail

A moribund, emaciated California quail (Callipepla californica) that was found in an orchard in the state of Washington had an impacted crop and gizzard. Pellets containing the anticoagulant chlorophacinone (Rozol, RO) were in the crop; the gizzard contents consisted of a pink mass of paraffin that was selectively accumulated from the paraffinized pellets. The plasma prothrombin time of 28 sec was near that determined for control quail. The signs of RO intoxication seen in the moribund wild quail were duplicated in captive quail given ad libitum diets of either RO or another paraffinized chlorophacinone pellet (Mr. Rat Guard II, MRG). This left little doubt that paraffin impaction of the gizzard was the primary problem. All captive quail fed RO or MRG pellets showed no increases in prothrombin times compared to control values, died in an emaciated condition, and had gizzards impacted with paraffin.

Journal of Wildlife Diseases↗

Yohimbine hydrochloride as an antagonist to xylazine hydrochloride-ketamine hydrochloride immobilization of white-tailed deer

Thirteen captive and one free-ranging white-tailed deer (Odocoileus virginianus) were immobilized one to six times each with ketamine hydrochloride and xylazine hydrochloride during winter and spring in northern Minnesota. Administration of 0.09 to 0.53 mg of yohimbine hydrochloride per kg IV after each trial reversed the immobilization. The deer raised their heads within a median time of 2.0 min, stood in 6.0 min and walked away in 9.5 min. No adverse side effects were observed for several weeks following the immobilization.

Journal of Wildlife Diseases↗

Quantitative and qualitative studies of gut flora in striped bass from estuarine and coastal marine environments

Examination of the intestinal contents of 130 striped bass ( Morone saxatilis ) collected from the Hudson River and Long Island Sound during May to October 1981 showed that opportunistic fish pathogens—especially Aeromonas hydrophila —predominated in samples from both locations. Other isolates from both groups of striped bass included Vibrio , pseudomonads, flavobacteria, Alcaligenes , and enterics. Small numbers of Micrococcus , Bacillus , Corynebacterium , and Acinetobacter were also isolated. Total numbers of bacteria in the intestines were 100 to 1,000 times higher in striped bass from the Hudson River than in those from Long Island Sound.

Journal of Wildlife Diseases↗

Immunoblot assay: a rapid and sensitive method for identification of salmonid fish viruses

An immunoblot assay was used to identify the viruses of infectious pancreatic necrosis, infectious hematopoietic necrosis, and viral hemorrhagic septicemia. Viral antigen in infected cell culture supernatant was adsorbed onto nitrocellulose membrane or Whatman 541 filter paper and detected by enzyme-linked immunosorbent assay techniques. The immunoblot assay took less than 4 hr to perform and required no special instrumentation. Assays using cell culture supernatant fluids showed immunoblot sensitivity was 10 5 –10 6 PFU/ml. Assay sensitivity, determined using purified virus, is 0.85–4.0 ng of viral antigen. The immunoblot assay was used to detect and identify virus in cell culture fluids.

Journal of Wildlife Diseases↗

Acute oral toxicity of sodium cyanide in birds

Sensitivities of six avian species, black vulture ( Coragyps atratus ), American kestrel ( Falco sparverius ), Japanese quail ( Coturnix japonica ), domestic chicken ( Gallus domesticus ), eastern screech-owl ( Otus asio ), and European starling ( Sturnus vulgaris ), to acute poisoning by sodium cyanide (NaCN) were compared by single dose LD50's. Three species, domestic chickens, black vultures, and turkey vultures ( Cathartes aura ), were dosed with NaCN to determine cyanide residues in those that died and also in survivors, in addition to postmortem fate. Three flesh-eating species (black vulture, American kestrel, and eastern screech-owl; LD50's 4.0–8.6 mg/kg) were more sensitive to NaCN than three species (Japanese quail, domestic chicken, and European starling; LD50's 9.4–21 mg/kg) that fed predominantly on plant material. Elevated concentrations of cyanide were found in the blood of birds that died of cyanide poisoning; however, concentrations in birds that died overlapped those in survivors. Blood was superior to liver as the tissue of choice for detecting cyanide exposure. No gross pathological changes related to dosing were observed at necropsy.

Journal of Wildlife Diseases↗

Case histories of bald eagles and other raptors killed by organophosphorus insecticides topically applied to livestock

Since 1982 when secondary poisoning of a red-tailed hawk ( Buteo jamaicensis ) was documented following the recommended use of famphur applied topically to cattle, the Patuxent Wildlife Research Center has tested dead birds of prey for poisoning by famphur and other pouron organophosphorus (OP) insecticides. Brain cholinesterase (ChE) activity was first determined, then if ChE was depressed ≥50%, stomach and/or crop contents were evaluated for anti-ChE compounds. This report presents the circumstances surrounding the OP-caused deaths of eight bald eagles ( Haliaeetus leucocephalus ), two red-tailed hawks, and one great horned owl ( Bubo virginianus ) between March 1984 and March 1985. OP poisoning of raptors by pour-on insecticides in the United States is widespread, but its magnitude is unknown.

Journal of Wildlife Diseases↗

Lead poisoning in Canada geese on Plum Island, Massachusetts (USA)

During December 1983 and early January 1984, about 200 Canada geese (Branta canadensis) died of lead poisoning at Parker River National Wildlife Refuge on Plum Island, Massachusetts. In an effort to determine the source of lead, 100 bottom samples were taken from a refuge impoundment where much of the mortality/morbidity occurred. An average of 157,150 pellets/ha was found with a range of 64,582 to 322,910 pellets/ha. Water levels in this impoundment were low when Canada geese arrived, making shot more readily available to the geese and contributing to the outbreak. To minimize the risk of Canada geese being exposed to lead shot poisoning at this location in the future, we recommend several corrective manipulations of habitat.

Massachusetts↗

Salt toxicosis in waterfowl in North Dakota

About 150 waterfowl died and another 250 became weak and lethargic from suspected salt poisoning after using White Lake, a highly saline lake in Mountrail County, North Dakota. Frigid temperatures made fresh water unavailable, forcing the birds to ingest the saline waters with resultant toxic effects. Sick birds recovered when removed from the salt water and released into fresh water marshes. Brain sodium levels were higher in dead geese submitted for necropsy than in controls.

North Dakota↗

Cardiovascular and behavioral responses of gray wolves to ketamine-xylazine immobilization and antagonism by yohimbine

Adult wolves ( Canis lupus ) were immobilized with 6.6 mg/kg ketamine hydrochloride (KET) and 2.2 mg/kg xylazine hydrochloride (XYL) administered intramuscularly. Induction time was 4.6 ± 0.3 min ( x̄ ± SE). Immobilization resulted in significant bradycardia and hypertension ( P < 0.05). Twenty min after induction, the wolves were given 0.05–0.60 mg/kg yohimbine hydrochloride (YOH). Yohimbine given intravenously produced dose-related increases in heart rate (HR) with doses >0.15 mg/kg resulting in extreme tachycardia (>300 bpm). All doses of YOH caused a temporary decrease in mean arterial blood pressure (MABP) with some individual animals manifesting profound hypotension (<30 torr) at doses >0.15 mg/kg. Increasing the dose of YOH above 0.15 mg/kg did not significantly decrease either arousal or ambulation times. Administering YOH at 40 or 60 min after induction resulted in decreased arousal and ambulation times. Stimulation by weighing and taking repeated blood samples during anesthesia did not shorten arousal times. We recommend that wolves immobilized with XYL-KET be antagonized with doses of YOH <0.15 mg/kg.

Journal of Wildlife Diseases↗

A solitary case of duck plague in a wild mallard

Duck plague was diagnosed on the basis of pathology and virus isolation in a wild female mallard Anas platyrhynchos found dead near Saskatoon, Saskatchewan. Day-old Pekin ducklings and one of two adult mallards died with lesions typical of duck plague following inoculation of tissue from the wild bird. This is believed to be the only reported case of duck plague in a wild bird since a major outbreak occurred in South Dakota in 1973, and the fourth such report in North America.

Saskatchewan↗

Antibody response of sandhill and whooping cranes to an eastern equine encephalitis virus vaccine

As a possible strategy to protect whooping cranes (Grus americana) from fatal eastern equine encephalitis (EEE) viral infection, studies were conducted to determine the immune response of this species and sandhill cranes (Grus canadensis) to a formalin-inactivated EEE viral vaccine. Viral-specific neutralizing antibody was elicited in both species after intramuscular (IM) vaccination. Subcutaneous and intravenous routes of vaccination failed to elicit detectable antibody in sandhill cranes. Among the IM vaccinated cranes, the immune response was characterized by nondetectable or low antibody titers that waned rapidly following primary exposure to the vaccine. However, one or more booster doses consistently elicited detectable antibody and/or increased antibody titers in the whooping cranes. In contrast, cranes with pre-existing EEE viral antibody, apparently induced by natural infection, exhibited a rapid increase and sustained high-antibody titers. Even though EEE virus vaccine induced neutralizing antibody and produced no adverse side effects, further studies will be required to determine the protective efficacy of the antibody.

Journal of Wildlife Diseases↗

Immunization of salmonids against Yersinia ruckeri: Significance of humoral immunity and cross protection between serotypes

Brook trout ( Salvelinus fontinalis ) were immunized with bacterins containing either Serotype 1 or 2 isolates of Yersinia ruckeri to determine the relative degree of cross-protection afforded when the fish were challenged with the homologous or heterologous serotype. While fish immunized with pH-lysed bacterins produced highly specific agglutinins that did not cross-react with antigens derived from a heterologous serotype of Y. ruckeri all fish were protected against experimental challenge, regardless of which serotype was used for bacterin production and experimental challenge. Other experiments indicated that brook trout injected intraperitoneally with highly specific antibodies could not be passively immunized against experimental challenge.

Journal of Wildlife Diseases↗