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At least 361 records · Page 20Linked to original sources

Variation of cisco egg size among Laurentian Great Lakes populations

Many fish species display inter-population and inter-individual egg size variation. Intra-specific differences in egg size seemingly reflect both energetic experiences of individual spawning fish and long-term population responses to differing ecosystems. Optimal egg size theory implies that selection influences a population’s mean egg size in response to its early-life environment, given the well-established trade-off between egg size and fecundity. Currently, there is strong interest in rehabilitation of Laurentian Great Lakes cisco, Coregonus artedi, which is characterized by inter-population variation of morphological and behavioral traits. However, the extent of cisco egg size variation is under-described. In fall 2018 and 2019, we collected egg samples by stripping ripe females at seven total locations in four Great Lakes. We measured unfertilized egg diameters using imaging software and compared mean egg diameters among locations with and without including maternal total length as a covariate. Lake Michigan females produced the largest eggs overall but were excluded from analyses using the total length covariate because of their significantly larger body sizes. Maternal length had a positive effect on egg size, and when accounting for this effect, females in Lake Huron produced the largest eggs followed by Lake Ontario and Lake Superior. We also found that egg size varied among locations within Lake Superior. These findings aligned with observations of morphological and behavioral differences among populations and suggest that cisco phenotypic variation at a fine spatial scale extends to reproductive biology. Consideration of cisco reproductive traits, such as egg size, may inform restoration strategies, including supplemental stocking.

Journal of Great Lakes Research↗

Effects of warming winter embryo incubation temperatures on larval cisco (Coregonus artedi) survival, growth, and critical thermal maximum

Freshwater whitefishes, Salmonidae Coregoninae, are cold stenothermic fishes of ecological and socio-economic importance in northern hemisphere lakes that are warming in response to climate change. To address the effect of warming waters on coregonine reproduction we experimentally evaluated different embryo incubation temperatures on post-hatching survival, growth, and critical thermal maximum of larval cisco ( Coregonus artedi ) sampled from lakes Superior and Ontario. Embryos were incubated at water temperatures of 2.0, 4.4, 6.9, and 8.9 °C to simulate present and increased winter temperatures, and hatched larvae were reared in a common environment. For the populations from both lakes, larval survival and critical thermal maximum were negatively related to incubation temperature, and larval growth was positively related to incubation temperature. The magnitude of change across incubation temperatures was greater in the population sampled from Lake Superior than Lake Ontario for all traits examined. The more rapid decrease in survival and critical thermal maximum across incubation temperatures for larval cisco in Lake Superior, compared to those from Lake Ontario, suggests that Lake Superior larvae may possess a more limited ability to acclimate to and cope with increasing winter water temperatures. However, the rapid increase in growth rates across incubation temperatures in Lake Superior larvae suggests they could recover better from hatching at a small length induced by warm winters, as compared to Lake Ontario larvae. Our results suggest propagation and restoration programs may want to consider integrating natural habitat preferences and maximizing phenotypic variability to ensure offspring are set up for success upon stocking.

Lake Ontario, Lake Superior↗

Genome-wide genetic diversity may help identify fine-scale genetic structure among lake whitefish spawning groups in Lake Erie

In Lake Erie, lake whitefish Coregonus clupeaformis supported lucrative fisheries before populations were decimated by overfishing and water quality degradation. In recent years, there has been a renewed interest in lake whitefish and management of the fishery they support. Lake whitefish spawn on several reefs throughout Lake Erie, but the relative recruitment dynamics and contributions of spawning groups to the fishery are not well understood. Modern high-throughput sequencing approaches offer new opportunities to census population diversity and to identify subtle differences among closely related populations. We used high-throughput sequencing data to evaluate the genetic structure and diversity of lake whitefish collected opportunistically across broad spatial scales in Lake Erie. Using RAD-capture (Rapture), we sequenced and genotyped individuals (N = 88) from the west, central, and east basin of Lake Erie at 120,268 single nucleotide polymorphisms (SNPs). Lake whitefish from Niagara and Crib Reefs (west basin) diverged from the three collections. Interestingly, these were the only lake whitefish collected during the act of spawning (late November), and all other fish were collected pre-spawn (August-early November). These results suggest that some lake whitefish spawning reefs may be reproductively isolated, though definition of these groups into stocks will require more intentional sampling during the act of spawning.

Michigan, New York, Ohio, Ontario, Pennsylvania↗

Additional deepwater sampling of prey fish in Lake Michigan annual bottom trawl survey reveals new insights for depth distribution dynamics

Beginning in 2013, sites at the 128-m bottom depth contour were added to the sampling design of the annual Lake Michigan bottom trawl survey for prey fish, which has been conducted by the U.S. Geological Survey Great Lakes Science Center (GLSC) each fall since 1973, to better assess fish depth distributions in a changing ecosystem. The standard sampling design included bottom depths from 9 to 110 m, although the GLSC also sporadically conducted bottom trawl tows at the 128-m bottom depth contour during 1973–1988. Enactment of this new sampling design in 2013 revealed that mean biomass density of deepwater sculpins ( Myoxocephalus thompsonii ) at the 128-m depth exceeded the sum of mean biomass densities at shallower depths, indicating that the bulk of the deepwater sculpin population is residing in waters deeper than 110 m. Thus, our findings supported the hypothesis that the depth distribution of the deepwater sculpin population had shifted to deeper waters beginning in 2007, thereby explaining, at least in part, the marked decline in deepwater sculpin abundance since 2006 based on the standard sampling design. In contrast, our results did not support the hypothesis that the slimy sculpin ( Cottus cognatus ) population had shifted to deeper waters sometime after 2000. A portion of the burbot (Lota lota) population may have also shifted in depth distribution to waters deeper than 110 m after 2007, based on our results. Our findings have served as an impetus to further expand the range of depths sampled in our bottom trawl survey.

Lake Michigan↗

Predicting physical and geomorphic habitat associated with historical lake whitefish and cisco spawning locations in Lakes Erie and Ontario

The Great Lakes basin was historically populated by multiple, coevolved coregonine species, but much of that diversity has been lost. In Lakes Erie and Ontario, both lake whitefish ( Coregonus clupeaformis ) and cisco ( Coregonus artedi ) occurred in high numbers before habitat degradation, overfishing, invasive species, and other factors caused significant declines. There is growing interest in restoring these populations, and suggested actions include restoration of critical habitats such as spawning habitat. Unfortunately, our current understanding of lake whitefish and cisco spawning habitat characteristics and locations in these lakes is limited. To highlight areas of potential importance for conservation and restoration, we used random forest models and data on historical spawning locations to predict lake whitefish and cisco spawning habitats based on hypothesized key factors including wind fetch, ice cover duration, distance from 1st and 6th order tributaries, and lake bottom substrate. Our model accurately predicted spawning habitat locations for 71% and 54% of cases for lake whitefish and cisco, respectively. Fetch was the most important variable in the lake whitefish model, with spawning habitats being most likely to occur in regions of low to moderate fetch. Cisco spawning habitats were most likely to occur in areas of relatively low fetch near a 1st order stream. We used these models to predict spawning habitat locations for both species across Lakes Erie, Ontario, and St. Clair. Our results improve our understanding of lake whitefish and cisco spawning habitat characteristics and will aid in the spatial prioritization of actions to restore these native fishes.

Lake Erie, Lake Ontario↗

Indicators of fish population responses to avian predation with focus on double-crested cormorants

Double-crested cormorants (Nannopterum auritum) have been implicated as causes of fish population declines in many locations across their breeding range. Two challenges facing managers are identifying fisheries population metrics indicative of cormorant impacts and determining when this evidence becomes actionable. Building upon existing studies, we conducted a meta-analysis of eight data-rich systems across the Laurentian Great Lakes region of the United States for common fish population responses to changes in cormorant abundance. Specifically, we examined trends in mean total female length at age-3 (TL3), female mean length and age at 50 % maturity, and mean age evenness as indicated by Shannon’s Equitability Index. Annual observations for these metrics were independently regressed linearly against cormorant density by system for walleye (Sander vitreus), yellow perch (Perca flavescens), smallmouth bass (Micropterus dolomieu), and northern pike (Esox lucius) populations. TL3 was the most sensitive with 9 of the 14 datasets statistically significant (r2 range 0.29 to 0.86). Maturity metrics were moderately sensitive to trends in cormorant predation with mean total length at 50 % maturity significant in 4 out of 11 datasets (r2 range 0.27–0.41) and mean age at 50 % maturity significant in 3 out of 11 datasets (r2 range 0.12 – 0.51). Least sensitive was age evenness with the Shannon Index significant in 3 out of 12 datasets (r2 typically < 0.25). Of metrics tested, TL3 was the most reliable indicator of changes in cormorant effects despite varying system changes and management responses among locations.

Michigan, Minnesota, New York↗

Stream corridor and upland sources of fluvial sediment and phosphorus from a mixed urban-agricultural tributary to the Great Lakes

Like many impaired Great Lakes tributaries, Apple Creek, Wisconsin (119 km 2 ) has Total Maximum Daily Load (TMDL) targets for reducing suspended sediment and total phosphorus by 51.2 % and 64.2 %, respectively. From August 2017 - October 2018, a stream sediment budget and fingerprinting integrated study was conducted to quantify upland and stream corridor sources of suspended sediment and sediment-bound phosphorus. Phosphorus concentrations varied among source groups and fluvial sediments, with higher concentrations among suspended sediment and cropland soils. Eroding streambanks identified in the stream corridor sediment budget accounted for 100 % of the TMDL Soil and Water Assessment Tool (SWAT) suspended sediment load but only 20 % of the total phosphorus load. Fine-grained streambed sediment equated to approximately-three years of modeled suspended sediment load but only one third of total phosphorus load. The two primary sources of fine-grained streambed sediment were streambanks and cropland, with relative streambank contributions increasing with downstream direction and watershed area. The relative proportion of suspended sediment varied by season and streamflow; however, cropland and streambank erosion accounted for 54 % and 23 % of the suspended sediment when weighted by of the proportion for representative streamflow. Urban land was a source in the upper watershed, but the signature was sequestered by a mid-watershed detention basin. Contributions from construction sites were higher in the fall 2018, likely corresponding to increased activity following a wet spring. These integrated techniques helped describe sources, transport, and sinks of fluvial sediment and phosphorus throughout the watershed at a range of spatial and temporal scales.

Wisconsin↗

An extrapolation method for estimating loads from unmonitored areas using watershed model load ratios

It is important to routinely estimate loads from an entire watershed to describe current conditions and evaluate how watershed-wide management efforts have affected the nutrient and sediment export that affect downstream water quality. However, monitoring in most areas, including the Great Lakes watershed, consists of sampling at a limited number of sites that are only periodically used to estimate total watershed loading. Here, we describe a technique to extrapolate loads measured at a limited number of reference sites to the total load from a large watershed using load ratios between monitored sites and unmonitored areas obtained from a watershed model (i.e., model load ratio, MLR, approach). In this study, modeled nonpoint-source load ratios between monitored tributaries (reference sites) and nearby unmonitored areas and point-source delivery factors for all areas were obtained from a Spatially Referenced Regression On Watershed attributes (SPARROW) model and used to extrapolate the measured loads from an ongoing monitoring program (Great Lakes Restoration Initiative Tributary monitoring program) to the entire Great Lakes watershed. The MLR approach incorporates spatial variability in nonpoint- and point-source delivery, watershed characteristics, and hydrology that are often not considered when estimating loads from unmonitored areas, such as using the unit area load (UAL) extrapolation approach. The MLR approach provided smaller watershed loads than the UAL approach because yields from monitored sites, in general, were larger than from unmonitored areas. When both approaches were used to estimate loads at adjacent monitored sites, the MLR approach provided more accurate estimates than the UAL approach.

Great Lakes Basin↗

Genetic population structure of cisco, Coregonus artedi, in the Laurentian Great Lakes

Management of a widely distributed species can be a challenge when management priorities, resource status, and assessment methods vary across jurisdictions. For example, restoration and preservation of coregonine species diversity is a goal of management agencies across the Laurentian Great Lakes. However, management goals and the amount of information available varies across management units, making the focus for management efforts challenging to determine. Genetic data provide a spatially consistent means to assess diversity. Therefore, we examined the genetic stock structure of cisco ( Coregonus artedi ) in the Great Lakes where the species is still extant. Using genotype data from 17 microsatellite DNA loci, we observed low levels of population structure among collections with most contributions to overall diversity occurring among lakes. Cisco from lakes Superior, Michigan, Ontario, and the St. Marys River could be considered single genetic populations while distinct genetic populations were observed among samples from northern Lake Huron. Significant within-lake diversity in Lake Huron is supported by populations found in embayments in northern Lake Huron. The Grand Traverse Bay population in Lake Michigan represents a distinct population with reduced levels of genetic variation when compared to other lakes. The different levels of within lake population structure we observed will be important to consider as future lake-specific management plans are developed.

Great Lakes↗

Does hatchery rearing of lake trout affect their reproductive behavior in the wild?

Stocking of hatchery-reared fishes has been used with variable success as a management action to promote the recovery of populations and species. The practice has been controversial for several reasons, including uncertainty about whether the hatchery rearing experience may affect reproduction after release. Fine-scale acoustic telemetry was used during three spawning seasons to test whether hatchery rearing affects the reproductive behavior of lake trout using a spawning shoal complex in northern Lake Huron. Within sex, wild- and hatchery-reared fish behaved similarly, but significant behavioral differences occurred between sexes. Lake trout of both sexes moved synchronously onto the spawning shoals at the completion of autumn thermal turnover and occupied the same spawning sites (confirmed visually by presence of fertilized eggs) on the shoals. Male lake trout tended to congregate directly on spawning sites, with duration of occupancy varying greatly among years. Female lake trout spent less time on spawning shoals than males and congregated less at spawning sites on shoals. Most fish visited multiple spawning sites among shoals per season, with many making multiple transits among individual spawning sites. We found no evidence to support the hypothesis that hatchery rearing impairs spawning behavior of lake trout and, therefore, conclude that behavior deficiencies on the spawning ground are likely not an impediment to rehabilitation of lake trout in northern Lake Huron. Our study narrows the field of possible impediments to lake trout rehabilitation in the Great Lakes and provides insights that expand the conceptual model of lake trout spawning behavior.

Michigan↗

Agricultural conservation practices could help offset climate change impacts on cyanobacterial harmful algal blooms in Lake Erie

Harmful algal blooms (HABs) are a recurring problem in many temperate large lake and coastal marine ecosystems, caused mainly by anthropogenic eutrophication. Implementation of agricultural conservation practices (ACPs) offers a means to reduce non-point source nutrient runoff and mitigate HABs. However, the effectiveness of ACPs in a changing climate remains uncertain. We used an integrated biophysical modeling approach to predict how Lake Erie cyanobacterial HAB severity (bloom biomass) may change under several climate and ACP implementation scenarios, using western Lake Erie and its largely agricultural watershed as our study system. An ensemble of general circulation model projections was used to drive spatially explicit land use and hydrology models of the Maumee River watershed, the output of which informed a predictive model of Lake Erie HAB severity. Results show that, in the absence of changes in ACPs, the frequency of severe HABs is projected to increase during coming decades, owing to increased inputs of nutrients from the watershed. These anticipated increases are due to increased total precipitation and more frequent higher-magnitude rainfall events. While further implementation of ACPs appears capable of reducing severe HAB events, widespread implementation would be necessary to reduce HAB severity below current management targets. This study highlights how continued climate change will only exacerbate the need for land management practices that can reduce nutrient runoff in agriculturally dominated ecosystems, such as Lake Erie. It also shows how interdisciplinary, biophysical modeling approaches can help identify strategies to mitigate HABs in the face of anthropogenic stressors.

Lake Erie↗

Assessing impaired benthic communities using sediment toxicity and contaminant concentrations from reference sites inside the Niagara River Area of Concern

Anthropogenically degraded benthic-macroinvertebrate communities (benthos) are one of seven beneficial use impairments (BUIs) in the Niagara River Area of Concern (AOC). Over the last 50 years, upgrades to waste-water treatment, industry closures, and sediment remediations reduced contaminant levels throughout the system. Improvements in benthic communities and sediment toxicity, however, were difficult to assess because there are no comparable reference reaches outside the AOC. A multi-phase study was initiated in 2015 to determine if data from inside the AOC could identify reference conditions, if toxicity and benthic-community data from these sites differed from other AOC sites, and if further remediation efforts were warranted in parts of the AOC. Concentrations or quotients of PAHs, PCBs, dioxins and furans, pesticides, and most metals were below their New York Sediment Class A Guidance Values at 10 sites that were subsequently designated as reference sites. Survival and growth data from Chironomus dilutus and Hyalella azteca bioassays indicated that sediments from only a few individual AOC-impact sites were toxic or significantly different from reference sites, and that mean toxicity at pooled AOC-impact and reference sites did not differ significantly. Similarly, New York Biological Assessment Profile scores and chironomid mentum deformity scores at only a few individual sites differed significantly from corresponding indices at reference sites, but neither metric differed significantly in comparisons between pooled AOC-impact and reference sites. Most analyses indicated that benthic communities were unimpaired and that removal criteria for the benthos BUI were largely met in much of the upper Niagara River AOC.

New York↗

Habitat selection of a migratory freshwater fish in response to seasonal hypoxia as revealed by acoustic telemetry

Adaptive efforts to achieve water quality objectives by modifying nutrient loading can have attendant impacts on fish habitats and fisheries. Thus, coordinating fishery and water quality management depends on knowledge of fish behavioral responses to habitat change. This study combined acoustic telemetry of fish with water quality modeling to understand how water quality management might impact fishery management. We examined habitat use of a native demersal fish, lake whitefish Coregonus clupeaformis , in Lake Erie. We focused on the summer stratified period when habitat was expected to be most limiting and used a forecast model to predict temperature and oxygen in the hypolimnion when fish were detected. As hypothesized, lake whitefish occupied a subset of available conditions with occupied habitats characterized by a cool, normoxic, hypolimnion. On some occasions fish were detected when the hypolimnion was predicted to be hypoxic, suggesting that fish were either displaced vertically or horizontally into marginal habitats or uncertainty in model predictions was high. Still, when hypolimnetic conditions were hypoxic, fish tended to move toward normoxia as expected, but when initial conditions were cold with high dissolved oxygen, fish movements were toward lower oxygen (but still normoxic) conditions. We also observed a high affinity for fish to remain near the southern shore in eastern Ohio, Pennsylvania, and New York. If current nutrient reduction objectives are achieved and the extent and severity of hypoxia is reduced, an expansion of lake whitefish habitat and distribution may have significance to the spatial regulation of fishing effort in Lake Erie.

Michigan, New York, Ohio, Pennsylvania↗

Lake Erie hypoxia spatial and temporal dynamics present challenges for assessing progress toward water quality goals

Seasonal hypolimnetic hypoxia has been documented in Lake Erie’s central basin since the 1950s. Ship-based surveys to monitor hypoxia have been conducted since the 1980s, but they occur at a relatively low frequency and focus on the deeper areas of the central basin. To better document the seasonal development of stratification and the consequent occurrence of hypoxia, we deployed eight moorings, in both nearshore-shallow areas and offshore-deep areas of the central basin, equipped with temperature and oxygen sensors at multiple depths, that recorded temperature and oxygen concentrations every 10 minutes. Results from 2017-2019 reveal that hypoxia occurs as early as July in the shallower areas west of, and around the southern perimeter of the central basin, but does not occur until August or September in the deeper central basin. Hypoxia is intermittent in the shallower perimeter areas; whereas in the deeper areas, hypoxia can persist into October, often progressing to anoxia. The intra and interannual differences in the spatial and temporal extent of hypoxia indicate that an extensive monitoring program will be necessary to more accurately assess progress toward reducing the extent of hypoxia pursuant to the lake ecosystem objectives of the 2012 Great Lakes Water Quality Agreement.

Michigan, New York, Ohio, Pennsylvania↗

Initial insights on the thermal ecology of lake whitefish in northwestern Lake Michigan

Lake whitefish Coregonus clupeaformis are a native coldwater species supporting important recreational and commercial fisheries in the Laurentian Great Lakes. Climate-related changes in water temperature may have important implications for the future sustainability of these fisheries. However, projecting future habitat availability is difficult because limited information is available on lake whitefish thermal ecology in the region. In this study, archival temperature loggers were implanted into 400 lake whitefish from northwestern Lake Michigan, including Green Bay, during October–November 2017. Loggers recorded temperature for 11 months at 4-hr intervals. Thirteen recovered temperature loggers were used in analyses. In winter (1 December–31 March), temperatures occupied by lake whitefish ranged from 0 to 8.0 °C, while in spring (1 April–31 May) temperatures ranged from 0 to 20.0 °C. In summer (1 June–15 September) and fall (16 September–7 November), lake whitefish occupied temperatures of 4–21.5 and 4–21.0 °C, respectively. Average temperatures in summer (10.8 °C) were within the previously proposed optimal temperature range (10–14 °C) and broad thermal niche (7–17 °C); however, 58% of observations were outside the optimal temperature range and 11% of observations were outside the broad thermal niche. Our results suggest that lake whitefish from northwestern Lake Michigan inhabit temperatures both above and below previously reported expected temperature ranges. This study provides initial insights on lake whitefish thermal ecology in Lake Michigan and can be used as a baseline for future work aimed at determining how lake whitefish habitat availability may change in the future.

Wisconsin↗

Future climate-induced changes in mixing and deep oxygen content of a caldera lake with hydrothermal heat and salt inputs

Vertical profiles of temperature, salinity and dissolved oxygen in Crater Lake, a caldera lake in the Oregon Cascade Range that receives hydrothermal inputs of heat and salt, were simulated with a 1-dimensional model. Twelve Global Circulation Models and two Representative Concentration Pathways (RCPs) were used to develop boundary conditions from 1950 to 2099. The model simulated the ventilation of deep water initiated by reverse stratification and subsequent thermobaric instability. All models predicted a reduction in the frequency of deep ventilation events, from an ensemble median frequency of 5.4 winters decade−1 during 1950–2005 to 4.3 (RCP4.5) or 2.5 (RCP8.5) winters decade−1 during 2045–2099. Favorable conditions for thermobaric instability-induced mixing currently occur infrequently and will become rare in the future. The salinity gradient resulting from hydrothermal inputs presents an additional barrier to thermobaric instability that will continue through 2099. A redistribution of salt to the deep lake may prevent ventilation all the way to the bottom in the future. Hypolimnetic dissolved oxygen percent saturation remained above 75% within the 21st century, consistent with oligotrophy and very small oxygen demands. The rate of change in all variables accelerated approaching 2099, coincident with elimination of winter reverse stratification. Historically, about half of the hydrothermal heat added to Crater Lake has been vented to the atmosphere. In the RCP8.5 scenario, the hydrothermal heat will cease to be vented to the atmosphere by the end of the 21st century, and then the temperature of the deep waters will increase rapidly.

Oregon↗

Factors influencing egg thiamine concentrations of Lake Ontario lake trout: 2019–2020

In the Great Lakes region, thiamine deficiency is considered a recruitment bottleneck for lake trout Salvelinus namaycush and has been correlated with the consumption of non-native alewife Alosa pseudoharengus . While alewife, the most abundant forage fish in Lake Ontario, are the predominant prey for lake trout, they also consume benthic prey such as round goby Neogobius melanostomus . Because variation in the proportion of alewife in lake trout diets is linked to variation in egg thiamine concentrations, understanding how factors such as region of capture and hatchery-strain of lake trout influence diet, are key to understanding the patterns of variation in egg thiamine concentrations observed in this species. With recent increases in natural recruitment of lake trout being observed in the western region of the lake, understanding if egg thiamine is a potential driver is crucial to the rehabilitation of lake trout. In this study, we evaluated egg thiamine concentrations in lake trout during 2019–2020. We found no significant difference in egg thiamine concentrations among regions. However, a stocked Lake Superior deepwater morphotype (Superior Klondike Wild – SKW) showed significantly higher egg thiamine concentrations compared to the lean morphotype including Seneca (SEN) and Lake Champlain Domestic (LCD) strains. An analysis of fatty acid signatures of each hatchery-strain suggested that the SKW strain consumed a higher proportion of round goby than lean strains. Overall, these results suggest that morphotypic differences in the feeding ecology of lake trout can result in biochemical changes which may influence the effectiveness of restoration efforts.

Lake Ontario↗

Large-scale variation in lakebed properties interpreted from single-beam sonar in two Laurentian Great Lakes

Acoustic seabed classification (ASC) is an important method for understanding landscape-level physical and biological patterns in the aquatic environment. Bottom habitats in the Laurentian Great Lakes are poorly mapped to date, and will require a variety of contributors and data sources to complete. We repurposed a long-term split-beam echosounder dataset gathered for purposes of fisheries assessment to estimate lakebed properties utilizing unsupervised classification of echo return data. We interpreted first echo properties representing lakebed hardness and roughness to define and map three statistically supported lakebed classes revealed through cluster analysis. Our results indicate coherent and logical class boundaries and suggest that the dataset has promise for expanded use in ASC. To improve inferences using repeated measures, future work should focus on collecting ground truth information for areas previously surveyed and on collecting concurrent ground truth information when sampling acoustic data moving forward.

Michigan, Ontario, Wisconsin↗