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Small-mammal density estimation: A field comparison of grid-based vs. web-based density estimators

Statistical models for estimating absolute densities of field populations of animals have been widely used over the last century in both scientific studies and wildlife management programs. To date, two general classes of density estimation models have been developed: models that use data sets from capture–recapture or removal sampling techniques (often derived from trapping grids) from which separate estimates of population size ( NÌ‚ ) and effective sampling area ( AÌ‚ ) are used to calculate density ( DÌ‚ = NÌ‚ / AÌ‚ ); and models applicable to sampling regimes using distance-sampling theory (typically transect lines or trapping webs) to estimate detection functions and densities directly from the distance data. However, few studies have evaluated these respective models for accuracy, precision, and bias on known field populations, and no studies have been conducted that compare the two approaches under controlled field conditions. In this study, we evaluated both classes of density estimators on known densities of enclosed rodent populations. Test data sets ( n = 11) were developed using nine rodent species from capture–recapture live-trapping on both trapping grids and trapping webs in four replicate 4.2-ha enclosures on the Sevilleta National Wildlife Refuge in central New Mexico, USA. Additional “saturation” trapping efforts resulted in an enumeration of the rodent populations in each enclosure, allowing the computation of true densities. Density estimates ( DÌ‚ ) were calculated using program CAPTURE for the grid data sets and program DISTANCE for the web data sets, and these results were compared to the known true densities ( D ) to evaluate each model's relative mean square error, accuracy, precision, and bias. In addition, we evaluated a variety of approaches to each data set's analysis by having a group of independent expert analysts calculate their best density estimates without a priori knowledge of the true densities; this “blind” test allowed us to evaluate the influence of expertise and experience in calculating density estimates in comparison to simply using default values in programs CAPTURE and DISTANCE. While the rodent sample sizes were considerably smaller than the recommended minimum for good model results, we found that several models performed well empirically, including the web-based uniform and half-normal models in program DISTANCE, and the grid-based models M b and M bh in program CAPTURE (with AÌ‚ adjusted by species-specific full mean maximum distance moved (MMDM) values). These models produced accurate DÌ‚ values (with 95% confidence intervals that included the true D values) and exhibited acceptable bias but poor precision. However, in linear regression analyses comparing each model's DÌ‚ values to the true D values over the range of observed test densities, only the web-based uniform model exhibited a regression slope near 1.0; all other models showed substantial slope deviations, indicating biased estimates at higher or lower density values. In addition, the grid-based DÌ‚ analyses using full MMDM values for WÌ‚ area adjustments required a number of theoretical assumptions of uncertain validity, and we therefore viewed their empirical successes with caution. Finally, density estimates from the independent analysts were highly variable, but estimates from web-based approaches had smaller mean square errors and better achieved confidence-interval coverage of D than did grid-based approaches. Our results support the contention that web-based approaches for density estimation of small-mammal populations are both theoretically and empirically superior to grid-based approaches, even when sample size is far less than often recommended. In view of the increasing need for standardized environmental measures for comparisons among ecosystems and through time, analytical models based on distance sampling appear to offer accurate density estimation approaches for research studies involving small-mammal abundances.

Ecological Monographs↗

[Book review] Return of the Whooping Crane

Fewer than 40 years ago, Life magazine ran an article decrying the plight of Whooping Cranes ( Grus americana ) on their wintering grounds at Aransas National Wildlife Refuge (Aransas) along the Gulf Coast. The small flock of approximately 20 birds that summered at Wood Buffalo National Park (Wood Buffalo) in Canada and wintered on the Texas coast at Aransas comprised the entire wild population of the species-a population that at the time seemed to be drifting inexorably to- ward extinction. Today, the Aransas/Wood Buffalo flock numbers more than 140 birds, there are more than 30 birds in captivity at the Patuxent Wildlife Research Center (Patuxent), and another 20-plus birds at the International Crane Foundation in Baraboo, Wisconsin. There are also a dozen wild birds in an experimental flock (termed the Rocky Mountain flock by Doughty) that winters at Bosque Del Apache National Wildlife Refuge (NWR) in New Mexico and summers in the mountain valleys centered on Grays Lake NWR in Idaho.

The Auk↗

U.S. Geological Survey science for the Wyoming Landscape Conservation Initiative—2018 annual report

The Wyoming Landscape Conservation Initiative (WLCI) was established in 2007 as a collaborative interagency partnership to develop and implement science-based conservation actions. During the past 11 years, partners from U.S. Geological Survey (USGS), State and Federal land management agencies, universities, and the public have collaborated to implement a long-term (more than 10 years) science-based program that assesses and enhances the quality and quantity of wildlife habitats in the southwest Wyoming region while facilitating responsible development. The USGS WLCI Science Team completes scientific research and develops tools that inform and support WLCI partner planning, decision making, and on-the-ground management actions. In fiscal year 2018, the USGS initiated 3 new projects and continued efforts on 21 ongoing science and web-development projects. The first new project was initiated to support Secretarial Order 3362 which calls on the USGS to assist Western States in mapping big-game migration corridors and developing new mapping tools. During 2018, the USGS hosted a workshop in Laramie, Wyoming, which included more than 70 State and Federal wildlife experts from Colorado, New Mexico, Texas, and Wyoming. Most of the mapping and migration tool curricula used in the workshop were derived from prior WLCI studies and mapping efforts of big-game migration movement in habitats undergoing large-scale energy development. The second new project was in response for WLCI partners to better understand sedimentation and hydrogeomorphic processes in a cold-desert headwater and the third new project was designed to improve our approach for people to access, manage, and analyze WLCI data and WLCI resource information. The USGS published 18 products (including peer-reviewed journal articles, USGS series publications, and data releases) and provided more than a dozen professional oral and poster presentations at scientific meetings and numerous informal presentations to WLCI partners at meetings and workshops. This report summarizes the objectives and status of each project and highlights the USGS 2018 accomplishments and products.

Wyoming↗

Use of ultralight aircraft for introducing migratory crane populations

Objectives were to determine if captive-reared cranes could be led behind an ultralight aircraft (UL) along a migration route and, if after release on a wintering area, they would integrate with wild cranes and migrate north in spring to their natal area without assistance. Greater sandhill cranes (Grus canadensis tabida) were used as the research surrogate for whooping cranes (Grus americana). In 1995, the senior author raised 15 cranes to fledging and trained them to respond to his vocal imitation of a sandhill crane brood call. Chicks learned to follow him as he walked, drove an all-terrain vehicle (ATV), or piloted an UL. The caretakers were not in crane costumes. Cranes were tame but allowed to roam at will without accompanying humans part of the day and were penned at night. Daily excursions provided exposure to habitats, foods, and predators the birds would encounter after release into the wild. In mid-October 1995, 11 radio-tagged cranes were led in migration from Grade, Idaho to Bosque del Apache National Wildlife Refuge (BdANWR), central New Mexico, and released near wild wintering sandhill cranes. The 1,204km migration took 11 days, including 1 day when the aircraft were grounded due to a winter storm. Hazards encountered enroute included mountainous terrain, turbulent air, and attacks by gold eagles (Aquila chrysaetos). On the wintering ground, hazards included crane hunters and coyotes (Canis latrans). Within 2 days after release at the BdANWR wintering site, the research cranes were associating with and imitating the behavior of wild cranes. The 4 surviving birds migrated north in spring 1996 and at the time of this writing 2 were within 53 km of their natal area in Idaho.

Book chapter↗

A systematic review of potential habitat suitability for the jaguar Panthera onca in central Arizona and New Mexico, USA

In April 2019, the U.S. Fish and Wildlife Service (USFWS) released its recovery plan for the jaguar Panthera onca after several decades of discussion, litigation and controversy about the status of the species in the USA. The USFWS estimated that potential habitat, south of the Interstate-10 highway in Arizona and New Mexico, had a carrying capacity of c. six jaguars, and so focused its recovery programme on areas south of the USA–Mexico border. Here we present a systematic review of the modelling and assessment efforts over the last 25 years, with a focus on areas north of Interstate-10 in Arizona and New Mexico, outside the recovery unit considered by the USFWS. Despite differences in data inputs, methods, and analytical extent, the nine previous studies found support for potential suitable jaguar habitat in the central mountain ranges of Arizona and New Mexico. Applying slightly modified versions of the USFWS model and recalculating an Arizona-focused model over both states provided additional confirmation. Extending the area of consideration also substantially raised the carrying capacity of habitats in Arizona and New Mexico, from six to 90 or 151 adult jaguars, using the modified USFWS models. This review demonstrates the crucial ways in which choosing the extent of analysis influences the conclusions of a conservation plan. More importantly, it opens a new opportunity for jaguar conservation in North America that could help address threats from habitat losses, climate change and border infrastructure.

Arizona, New Mexico↗

The 3D Elevation Program: summary for New Mexico

Elevation data are essential to a broad range of applications, including forest resources management, wildlife and habitat management, national security, recreation, and many others. For the State of New Mexico, elevation data are critical for infrastructure and construction management, natural resources conservation, flood risk management, agriculture and precision farming, geologic resource assessment and hazard mitigation, forest resources management, and other business uses. Today, high-density light detection and ranging (lidar) data are the primary sources for deriving elevation models and other datasets. Federal, State, Tribal, and local agencies work in partnership to (1) replace data that are older and of lower quality and (2) provide coverage where publicly accessible data do not exist. A joint goal of State and Federal partners is to acquire consistent, statewide coverage to support existing and emerging applications enabled by lidar data. The National Enhanced Elevation Assessment evaluated multiple elevation data acquisition options to determine the optimal data quality and data replacement cycle relative to cost to meet the identified requirements of the user community. The evaluation demonstrated that lidar acquisition at quality level 2 (table 1) for the conterminous United States and quality level 5 ifsar data (table 1) for Alaska with a 6- to 10-year acquisition cycle provided the highest benefit/cost ratios.The 3D Elevation Program (3DEP) initiative selected an 8-year acquisition cycle for the respective quality levels. 3DEP, managed by the U.S. Geological Survey (USGS), the Office of Management and Budget Circular A–16 lead agency for terrestrial elevation data, responds to the growing need for high-quality topographic data and a wide range of other 3D representations of the Nation’s natural and constructed features.

New Mexico↗

The 3D Elevation Program—Supporting New Mexico’s Economy

Introduction Federal, State, Tribal, and local entities managing lands in New Mexico have concerns about wildfire risk, wildlife habitat, and flood risk. Land managers in urban areas along the Rio Grande corridor and in the State’s rural northwest and southeast also have concerns about existing and developing roads, buildings, and other infrastructure. Federal, State, Tribal, and local organizations actively manage and monitor New Mexico’s water resources. Critical applications that meet the State’s management needs depend on light detection and ranging (lidar) data that provide a highly detailed three-dimensional (3D) model of the Earth’s surface and aboveground features. The 3D Elevation Program (3DEP) is managed by the U.S. Geological Survey in partnership with Federal, State, Tribal, U.S. territorial, and local agencies to acquire consistent lidar coverage at quality level 2 or better to meet the many needs of the Nation and New Mexico. The status of available and in-progress 3DEP baseline lidar data in New Mexico is shown. 3DEP baseline lidar data include quality level 2 or better, 1-meter or better digital elevation models, and lidar point clouds, and must meet the Lidar Base Specification version 1.2 or newer requirements. The National Enhanced Elevation Assessment identified user requirements and conservatively estimated that availability of lidar data would result in at least $9.32 million in new benefits annually to the State.

New Mexico↗

Using automated telemetry to identify population connectivity and migration phenology of Snowy Plovers breeding in the Southern Great Plains

Within-breeding season movements have not been quantified for Snowy Plovers ( Charadrius nivosus ) breeding on the Southern Great Plains (SGP), where suitable breeding habitat can range from less than 10 km to more than 600 km apart. This mosaic distribution of discrete patches of breeding habitat, combined with weather stochasticity and low densities of Snowy Plovers in Texas and New Mexico, increases the risk of local and regional extirpation. Further, little is known about SGP Snowy Plover migration phenology or winter habitat. We used the Motus Wildlife Tracking System to examine population connectivity, migration phenology, and winter habitat locations of adult Snowy Plovers in the SGP. Movements of Snowy Plovers during the 2017 and 2018 breeding seasons suggest little to no connectivity between the Salt Plains National Wildlife Refuge population in Oklahoma and populations in Texas and New Mexico. However, several Snowy Plovers in Texas moved to a lake formed by freshwater springs that may have provided higher-quality breeding and foraging habitat. Migrating primarily at night, we found that Snowy Plovers from a breeding area in Oklahoma made migratory movements to Texas and the Louisiana Gulf Coast. These data may be important to long-term conservation and planning efforts relative to understanding regional persistence and connectivity among breeding populations of Snowy Plovers in the SGP. Our results also highlight the need for future studies of wintering habitats used by SGP Snowy Plovers.

Journal of Field Ornithology↗

Detailed study of selenium and selected constituents in water, bottom sediment, soil, and biota associated with irrigation drainage in the San Juan River area, New Mexico, 1991-95

In response to increasing concern about the quality of irrigation drainage and its potential effects on fish, wildlife, and human health, the U.S. Department of the Interior began the National Irrigation Water Quality Program (NIWQP) to investigate these concerns at irrigation projects sponsored by the Department. The San Juan River area in northwestern New Mexico was one of the areas designated for study. Study teams composed of scientists from the U.S. Geological Survey, the U.S. Fish and Wildlife Service, the Bureau of Reclamation, and the Bureau of Indian Affairs collected water, bottom-sediment, soil, and biological samples at 61 sites in the San Juan River area during 1993-94. Supplemental data collection conducted during 1991-95 by the Bureau of Indian Affairs and its contractor extended the time period and sampling sites available for analysis. Analytical chemistry performed on samples indicated that most potentially toxic elements other than selenium generally were not high enough to be of concern to fish, wildlife, and human health. Element concentrations in some water, bottom-sediment, soil, and biological samples exceeded applicable standards and criteria suggested by researchers in current literature. Selenium concentrations in water samples from 28 sites in the study area exceeded the 2-microgramper-liter (lg/L) wildlife-habitat standard. Vanadium concentrations in water exceeded the 100-Kg/L standard for livestock-drinking water at one site. In biota, selenium and aluminum concentrations regularly equaled or exceeded avian dietary threshold concentrations. In bottom sediment and soil, element concentrations above the upper limit of the baseline range for western soils were: selenium, 24 exceedances; lead, 2 exceedances; molybdenum, 2 exceedances;strontium, 4 exceedances; and zinc, 4 exceedances. Concentrations of total selenium in bottom-sediment and soil samples were significantly greater for Cretaceous than for non-Cretaceous soil types in the study area and were generally similar for habitats within and outside irrigation-affected areas. Mean and median total-selenium concentrations in samples from areas with Cretaceous soil types were 4.6 and 2.2 micrograms per gram (ps/g), respectively. Mean and median total-selenium concentrations in samples from areas with non-Cretaceous soil types were 0.6 and 0.15 pg/g, respectively. Samples from the study area had low concentrations of organic constituents. Organochlorine pesticides and polychlorinated biphenyls were detected in a few biological samples at low concentrations. Polycyclic aromatic hydrocarbon (PAH) compounds were not detected in whole-water samples collected using conventional water-sampling techniques. In tests involving the use of semipermeable-membrane devices to supplement conventional water assays for PAH's, low concentrations of PAH's were found at several locations in the Hammond Irrigation Supply Canal, but were not detected in the Hammond ponds at the downstream reach of the Hammond irrigation service area. PAH compounds do not appear to reach the San Juan River through the Hammond Canal. Data indicate that water samples from irrigation-drainage-affected habitats had increased mean selenium concentrations compared with samples from irrigation-delivery habitat. The mean selenium concentration in water was greatest at seeps and tributaries draining irrigated land (17 μg/L); less in irrigation drains and in ponds on irrigated land (61.tg/L); and least in backwater, the San Juan River, and irrigation-supply water (0.5 - 0.6 μg/L). Statistical tests imply that irrigation significantly increases selenium concentrations in water samples when a Department of the Interior irrigation project is developed on selenium-rich sediments. Water samples from sites with Cretaceous soils had significantly greater selenium concentrations than water samples from sites with non-Cretaceous soils. Water samples from Department of the Interior project irrigation-drainage sites developed on Cretaceous soils contained a mean selenium concentration about 10 times greater than those in samples from Department of the Interior project sites developed on non-Cretaceous soils. Selenium was much less concentrated in water than in bottom sediment, soil, or biota in the study area. The range in concentrations of dissolved selenium in water was less than 1 ptg/L to 37 1.1g/L (less than 1 to 37 parts per billion). The range in concentrations of total selenium in bottom sediment and soil was less than 0.1 to 23lig/g (less than 100 to 23,000 parts per billion). The range in concentration of selenium in biota was less than 0.1 to 24.0 fig/g (less than 100 to 24,000 parts per billion). Data indicated that bioaccumulation and leaching from soil were the important processes at the study area that lead to elevated levels of selenium. Other processes examined included: (1) evapoconcentration of selenium; (2) atmospheric deposition of aerosols containing selenium; and (3) contamination of surface water by point-source or non-point-source discharges. Selenium concentrations in biological samples were evaluated by a number of variables including: (1) media sampled (emergent and submergent plants, nektonic and benthic invertebrates, omnivore/herbivore and carnivore fish, and terrestrial and aquatic amphibians); (2) habitat (San Juan River main-stem reaches, backwaters, tributary reaches, irrigation delivery or drainage canals, and ponds); (3) irrigation project area and reference sites; and (4) soil type (non-Cretaceous or Cretaceous soils). Graphical techniques and nonparametric statistical tests were applied to determine the influence of selected physiographic variables on selenium concentrations in biological samples collected in the San Juan River area. Species of sucker and of smaller fish contained significantly higher selenium concentrations in the upstream portion of the river where a productive community of plants and animals is found that is associated with warming, nutrient-rich waters discharged from an upstream reservoir. Selenium concentrations in algae, odonates, and mosquitofish collected from both irrigation-drain and pond habitats underlain by Cretaceous soils were significantly greater than in those collected from similar habitats underlain by non-Cretaceous soils. Investigators conclude that the major factor affecting the variability of selenium accumulation in biota at aquatic habitats was the presence of underlying Cretaceous soils. Median selenium concentrations were less than 2 lAg/g for plant samples, less than 7 μg/g for invertebrate samples, and less than 6 lAg/g for whole-fish samples collected from aquatic habitats underlain by non-Cretaceous soils. Similar samples collected from aquatic habitats underlain by Cretaceous soils contained median selenium concentrations two to five times greater. Leaching of selenium from Cretaceous soils in the San Juan River area increases the accumulation of selenium concentrations in the biota and thereby increases the exposure and potential health risks associated with selenium to migratory birds, fish, and other wildlife that use these aquatic habitats extensively. Aquatic habitats presenting the greatest average exposure to excess selenium concentrations in the diets of resident wildlife are from consumption of plants, invertebrates, and fish at irrigation-drain habitats underlain by Cretaceous soils. Of the irrigation projects evaluated in the San Juan River area, the highest median selenium concentrations in algae, cattail leaves, odonate nymphs, mosquitofish, and leopard frog samples from the study area were collected from the east hogback irrigation drain.

New Mexico↗

Characterization and load estimation of polychlorinated biphenyls (PCBs) from selected Rio Grande tributary stormwater channels in the Albuquerque urbanized area, New Mexico, 2017–18

In cooperation with the New Mexico County of Bernalillo, the U.S. Geological Survey characterized potential polychlorinated biphenyl (PCB) concentration and estimated loading into the Rio Grande from watersheds that are under the county’s jurisdiction. Water and sediment samples were collected in 2017–18 from six sites within four stormwater drainage basins in the Albuquerque, New Mexico, urbanized area for the analysis of PCB congeners and other water-quality constituents during dry and wet seasons. Also, the rainfall-runoff model Arid Lands Hydrologic Model (AHYMO) was used to estimate stormwater discharge at the two sample collection sites not affected by pump station operation. Along with the PCB analysis, the discharge data were used to estimate total PCB stormflow event loads for eight events in these urban Rio Grande tributaries. PCBs were detected in 34 of 36 water samples at concentrations as high as 65.8 nanograms per liter and in 12 of 13 sediment samples at concentrations as high as 163,000 nanograms per kilogram dry weight. Six of the 36 water samples exceeded the New Mexico surface-water quality standard for protection of wildlife habitat and aquatic life of 14 nanograms per liter for PCBs. None of the water samples exceeded the U.S. Environmental Protection Agency’s National Pollutant Discharge Elimination System permit level limit of 200 nanograms per liter for PCBs in stormwater systems discharging into the Rio Grande. PCB concentrations in water samples in this study were not linearly related to antecedent precipitation or measured water-quality parameters, but PCB concentrations had a statistically significant positive Kendall’s tau correlation with total suspended solids for water samples and with total organic carbon for sediment samples. The PCB congener profiles indicate that sources to stormwater drainage basins in Bernalillo County originate both from legacy sources, such as Aroclors (for example, in landfills and old building materials), and from current-use sources, such as yellow pigments (for example, in printed materials and packaging in urban litter or refuse). Total PCB stormflow event loads were calculated with average potential minimum and maximum event loads of 0.73 and 4.32 milligrams per storm event, respectively, at the Adobe Acres pump station site and 56.78 and 315.13 milligrams per storm event at the Sanchez Farms inflow at Albuquerque, N. Mex., site.

New Mexico↗

Physical, chemical, and biological data for detailed study of irrigation drainage in the San Juan River area, New Mexico, 1993-94, with supplemental data, 1991-95

In response to increasing concern about the quality of irrigation drainage and its potential effects on fish, wildlife, and human health, the U.S. Department of the Interior formed an interbureau task group to prepare a plan for investigating water- quality problems on irrigation projects sponsored by the Department of the Interior. The San Juan River area in northwestern New Mexico was one of the areas designated for study. Investigators collected water, bottom-sediment, soil, and biological samples at more than 50 sites in the San Juan River area during 1993-94. Sample sites included (1) sites located within Department of the Interior irrigation project service areas, or areas that receive drainage from irrigation projects; (2) reference sites for comparison with irrigation project sites; and (3) sites located within the reach of the San Juan River from Navajo Dam to 10 miles downstream from the dam. The types of habitat sampled included the main stem of the San Juan River, backwater areas adjacent to the San Juan River, tributaries to the San Juan River, ponds, seeps, irrigation-delivery canals, irrigation-drainage canals, a stock tank, and shallow ground water. The types of media sampled included water, bottom sediment, soil, aquatic plants, aquatic invertebrates, amphibians, and fish. Semipermeable-membrane devices were used as a surrogate medium to sample both air and water in some instances. Sample measurements included concentrations of major ions, trace elements, organochlorine pesticides, polychlorinated biphenyls, polycyclic-aromatic-hydrocarbon compounds, and stable isotopes of hydrogen and oxygen. This report presents tables of physical, chemical, and biological data collected for the U.S. Department of the Interior National Irrigation Water-Quality Program. Additionally, supplemental physical, chemical, and biological data collected in association with the Navajo Indian Irrigation Project are presented.

Open-File Report↗

Survival and cause-specific mortality of translocated female mule deer in southern New Mexico, USA

Context: Many mule deer ( Odocoileus hemionus ) populations in New Mexico have failed to recover from previous population declines, while some populations near urban areas have increased, resulting in more frequent human–wildlife conflicts. Translocations were used in an effort to simultaneously reduce an urban mule deer population and augment two low-density populations in south-western New Mexico, USA. Aims: Because of insufficient monitoring, the efficacy of many ungulate translocations is unknown. Our goal was to monitor cause-specific mortality and 1 year post-release survival of mule deer translocated during 2013 and 2014. We compared survival rates of mule deer released with a hard- versus soft-release during the 2014 translocation. Methods: . We translocated 218 mule deer in 2013 and 2014 into the Peloncillo Mountains (PM) and San Francisco River Valley (SFRV); 106 adult female mule deer were fitted with telemetry collars to determine cause-specific mortality and estimate survival 1 year post-release. All deer were hard-released in 2013. In 2014, translocated mule deer were either held in a soft-release pen (0.81 ha) for approximately 3 weeks or hard-released into their new environment. We used a Kaplan–Meier approach to estimate survival of translocated mule deer at each release area and to compare survival of mule deer translocated using each release method (i.e. hard- versus soft-release). Key results: In 2013–14, survival of hard-released deer in the PM was 0.627 (s.e. = 0.09), compared with 0.327 (s.e. = 0.10) in the SFRV. In 2014–15, survival of hard–released deer in the PM was 0.727 (s.e. = 0.13) and survival of soft-released deer was 0.786 (s.e. = 0.11). In the SFRV, survival of hard- and soft-released deer was 0.656 (s.e. = 0.14) and 0.50 (s.e. = 0.16), respectively. Causes of mortality were predation (51%), potential disease (9%; blue tongue or epizootic haemorrhagic disease), accident (5%), poaching (5%) and unknown (20%). Conclusions: Translocations can be an effective management tool to augment populations of mule deer while reducing overabundant urban populations. Soft-released mule deer did not have higher survival than hard-released mule deer, although the length and conditions of the acclimation period were limited in our study. Implications: Overabundant mule deer populations in urban areas may serve as sources of animals to bolster declining populations. Soft-release pens of smaller size and short period of acclimation did not influence survival.

New Mexico↗

Investigation into avian mortality in the Playa Lakes region of southeastern New Mexico: Final Report - June 1997

This Final Report is a review of work on a cooperative study undertaken by the USGS Biological Resources Division's National Wildlife Health Center (NWHC) and National Wetlands Research Center (NWRC; formerly the Southern Science Center) from 1994 through 1997. The study was initiated at the request of the Bureau of Land Management (BLM), through a request to the former National Biological Service. The Southeastern New Mexico Playa Lakes Coordinating Committee (SENMPLCC) played an important role in outlining the research needs. The initial Study Plan document, which outlines the background, objectives and methods for the first two years is available as Appendix 1. A letter indicating modifications to the Study Plan was sent to the SENMPLCC chair on April 25,1995, and is Appendix 2. An Interim Report, covering this work was completed and submitted in September 1995. The Literature Review section of the study was completed and presented to SENMPLCC in August, 1995. Following SENMPLCC review, NWHC was asked to develop a series of questions that could be posed from information gained in the initial phase (Appendix 3). The NWHC and NWRC were then directed to begin work to answer the top three questions, within the available fiscal resources. NWRC could continue with work outlined under the original Study Plan (Appendix 1), however an additional Study Plan for experiments performed by NWHC and collaborators and is available as Appendix 4.

New Mexico↗

Neonate morphometrics and lambing season characteristics of desert bighorn sheep

Desert bighorn sheep ( Ovis canadensis ) populations often occur in remote areas at low densities, leading to gaps in knowledge of life history. In November 2011, we translocated 11 female desert bighorn sheep from the Fra Cristobal Mountains and 9 from Red Rock Wildlife Management Area (RRWMA) to the Peloncillo Mountains in southwestern New Mexico. In December 2012, we captured 21 adult females in the Peloncillo Mountains, 14 of which were recaptured from 2011. We fitted each animal with a very high frequency (VHF) collar and vaginal implant transmitter (VIT) to monitor for parturition. We captured 26 lambs (5 females, 7 males in 2012; 7 males, 7 females in 2013), recorded morphometric measurements and fitted lambs with VHF collars to monitor survival. Over the study, 14 lambs died, with 12 mortalities from predation, one from abandonment, and one from unknown causes. Lambing season was protracted over 3–4 months and survival was unrelated to birth timing. Body mass differences between sex varied by year, suggesting a connection to annual climate. Because most studies focus on captive animals with access to supplemental food, captive lambs may not be representative of free-ranging populations. Thus, we investigated morphological trends in a free-ranging population.

Mammalia↗

Cooperative Fish and Wildlife Research Units Program—2017 year in review

The Cooperative Fish and Wildlife Research Units Program was involved in a number of notable events during 2017, many concerning our personnel. Dr. Barry Grand left his position as Leader of the Alabama Cooperative Fish and Wildlife Research Unit to become the Cooperative Units Program Supervisor for the South, replacing Dr. Kevin Whalen who took over as Supervisor for the West. We welcomed Dr. Sarah Converse who left the Patuxent Wildlife Research Center to become Leader of the Washington Cooperative Fish and Wildlife Research Unit. Dr. Amanda Rosenberger joined the Tennessee Cooperative Fishery Research Unit as Assistant Leader, transferring from the Missouri Cooperative Unit. Dr. Scott Carleton left his position as Assistant Unit Leader in New Mexico to become Chief of the Region 2 Migratory Bird Program of the U.S. Fish and Wildlife Service. We said farewell to many colleagues who retired. Their departure is bittersweet as we wish them health, happiness, and wellness in retirement. We will miss their companionship and the extraordinary contributions they have made to the Cooperative Fish and Wildlife Research Units Program and conservation. The Cooperative Fish and Wildlife Research Units Program has a record high number of vacant scientist positions due to a combination of retirements and base funding short-falls. These issues are affecting our ability to meet cooperator needs. Yet, we remain highly productive. For example, this year we released a report ( https://doi.org/10.3133/cir1427 ) containing abstracts of nearly 600 of our research projects, covering thematic areas ranging from advanced technologies to wildlife diseases. We provided highly competent, trained scientists and natural resource managers for our cooperators’ workforce. We delivered technical training and guidance to professional practitioners. We provided critical information to cooperators for decisions on species status assessments and management of species of greatest conservation need. This year we had an active presence at major national meetings, including the North American Wildlife and Natural Resources Conference where we co-sponsored a workshop on continuing education as a means to bridge the gap between science and management. During the coming year, with support from the U.S. Geological Survey and our cooperators, we intend to reduce the number of vacancies in the program. It will take time and active support of our cooperators to get back to full strength, but I am committed to this goal and encouraged by the resolve of our partners. We look forward to an even more productive year in 2018!

Circular↗

Behavioral, clinical, and pathological characterization of acid metalliferous water toxicity in mallards

From September to November 2000, United States Fish and Wildlife Service biologists investigated incidents involving 221 bird deaths at 3 mine sites located in New Mexico and Arizona. These bird deaths primarily involved passerine and waterfowl species and were assumed to be linked to consumption of acid metalliferous water (AMW). Because all of the carcasses were found in or near pregnant leach solution ponds, tailings ponds, and associated lakes or storm water retention basins, an acute-toxicity study was undertaken using a synthetic AMW (SAMW) formulation based on the contaminant profile of a representative pond believed to be responsible for avian mortalities. An acute oral-toxicity trial was performed with a mixed-sex group of mallards ( Anas platyrhynchos ). After a 24-h pretreatment food and water fast, gorge drinking was evident in both SAMW treatment and control groups, with water consumption rates greatest during the initial drinking periods. Seven of nine treated mallards were killed in extremis within 12 h after the initiation of dose. Total lethal doses of SAMW ranged from 69.8 to 270.1 mL/kg (mean ± SE 127.9 ± 27.1). Lethal doses of SAMW were consumed in as few as 20 to 40 min after first exposure. Clinical signs of SAMW toxicity included increased serum uric acid, aspartate aminotransferase, creatine kinase, potassium, and P levels. PCV values of SAMW-treated birds were also increased compared with control mallards. Histopathological lesions were observed in the esophagus, proventriculus, ventriculus, and duodenum of SAMW-treated mallards, with the most distinctive being erosion and ulceration of the kaolin of the ventriculus, ventricular hemorrhage and/or congestion, and duodenal hemorrhage. Clinical, pathological, and tissue-residue results from this study are consistent with literature documenting acute metal toxicosis, especially copper (Cu), in avian species and provide useful diagnostic profiles for AMW toxicity or mortality events. Blood and kidney Cu concentrations were 23- and 6-fold greater, respectively, in SAMW mortalities compared with controls, whereas Cu concentrations in liver were not nearly as increased, suggesting that blood and kidney concentrations may be more useful than liver concentrations for diagnosing Cu toxicosis in wild birds. Based on these findings and other reports of AMW toxicity events in wild birds, we conclude that AMW bodies pose a significant hazard to wildlife that come in contact with them.

New Mexico, Arizona↗

Biomonitoring of Environmental Status and Trends (BEST) Program: Environmental contaminants, health indicators, and reproductive biomarkers in fish from the Colorado River basin

Seven fish species were collected from 14 sites on rivers in the Colorado River Basin (CDRB) from August to October 2003. Spatial trends in the concentrations of accumulative contaminants were documented and contaminant effects on the fish were assessed. Sites were located on the mainstem of the Colorado River and on the Yampa, Green, Gunnison, San Juan, and Gila Rivers. Common carp (Cyprinus carpio), black bass (Micropterus sp.), and channel catfish (Ictalurus punctatus) were the targeted species. Fish were field-examined for external and internal anomalies, selected organs were weighed to compute somatic indices, and tissue and fluid samples were preserved for fish health and reproductive biomarker analyses. Composite samples of whole fish, grouped by species and gender, from each site were analyzed for organochlorine and elemental contaminants using performance-based and instrumental methods. 2,3,7,8-tetrachlorodibenzo-p-dioxin-like activity (TCDD-EQ) was measured using the H4IIE rat hepatoma cell bioassay. Selenium (Se) and mercury (Hg) concentrations were elevated throughout the CDRB, and pesticides concentrations were greatest in fish from agricultural areas in the Lower Colorado River and Gila River. Selenium concentrations exceeded toxicity thresholds for fish (>1.0 ?g/g ww) at all sites except from the Gila River at Hayden, Arizona. Mercury concentrations were elevated (>0.1 ?g/g ww) in fish from the Yampa River at Lay, Colorado; the Green River at Ouray National Wildlife Refuge (NWR), Utah and San Rafael, Utah; the San Juan River at Hogback Diversion, New Mexico; and the Colorado River at Gold Bar Canyon, Utah, Needles, California, and Imperial Dam, Arizona. Concentrations of p,p'-DDE were relatively high in fish from Arlington, Arizona (>1.0 ?g/g ww) and Phoenix, Arizona (>0.5 ?g/g ww). Concentrations of other banned pesticides including toxaphene, total chlordanes, and dieldrin were also greatest at these two sites but did not exceed toxicity thresholds. Current-use or unlisted pesticides such as dacthal, endosulfan, '-HCH, and methoxychlor were also greatest in fish from Gila River. Total polychlorinated biphenyls (PCBs; >0.11 ?g/g ww) and TCDD-EQs (>5 pg/g ww) exceeded wildlife guidelines in fish from the Gila River at Phoenix, Arizona. Hepatic ethoxyresorufin O-deethylase (EROD) activity was also relatively high in carp from the Gila River at Phoenix, Arizona and in bass from the Green River at Ouray NWR, Utah. Altered biomarkers were noted in fish throughout the CDRB. Fish from some stations responded to chronic contaminant exposure as indicated by fish health and reproductive biomarker results. Multiple fish health indicators including altered body and organ weights and high health assessment index scores may be associated with elevated Se concentrations in fish from the Colorado River at Loma, Colorado and Needles, California. Although grossly visible external or internal lesions were found on most fish from some sites, histopathological analysis determined many of these to be inflammatory responses associated with parasites. Edema, exophthalmos, and cataracts were noted in fish from sites with elevated Se concentrations. Reproductive biomarkers including gonad development and maturation, vitellogenin concentrations, and steroid hormone concentrations were anomalous in fish from the Gila River at Hayden and Phoenix, Arizona. In addition, intersex fish were found at seven of 14 sites. The intersex condition was identified in smallmouth bass (M. dolomieu), largemouth bass (M. salmoides), channel catfish, and carp and may indicate exposure to endocrine disrupting compounds. Seven of ten male smallmouth bass from the Yampa River at Lay, Colorado were intersex. Male carp, bass, and channel catfish with low concentrations of vitellogenin were common in the CDRB. Comparatively high vitellogenin concentrations (>0.2 mg/mL) were measured in male fish from the Green River at Ouray NWR, Utah and the Colorado River at Im

Scientific Investigations Report↗