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USGS2018

Malassezia vespertilionis sp. nov.: A new cold-tolerant species of yeast isolated from bats

Malassezia is a genus of medically-important, lipid-dependent yeasts that live on the skin of warm-blooded animals. The 17 described species have been documented primarily on humans and domestic animals, but few studies have examined Malassezia species associated with more diverse host groups such as wildlife. While investigating the skin mycobiota of healthy bats, we isolated a Malassezia sp. that exhibited only up to 92 % identity with other known species in the genus for the portion of the DNA sequence of the internal transcribed spacer region that could be confidently aligned. The Malassezia sp. was cultured from the skin of nine species of bats in the subfamily Myotinae ; isolates originated from bats sampled in both the eastern and western United States. Physiological features and molecular characterisation at seven additional loci (D1/D2 region of 26S rDNA, 18S rDNA, chitin synthase, second largest subunit of RNA polymerase II, β-tubulin, translation elongation factor EF-1α, and minichromosome maintenance complex component 7) indicated that all of the bat Malassezia isolates likely represented a single species distinct from other named taxa. Of particular note was the ability of the Malassezia sp. to grow over a broad range of temperatures (7–40 °C), with optimal growth occurring at 24 °C. These thermal growth ranges, unique among the described Malassezia , may be an adaptation by the fungus to survive on bats during both the host's hibernation and active seasons. The combination of genetic and physiological differences provided compelling evidence that this lipid-dependent yeast represents a novel species described herein as Malassezia vespertilionis sp. nov. Whole genome sequencing placed the new species as a basal member of the clade containing the species M. furfur , M. japonica , M. obtusa , and M. yamatoensis . The genetic and physiological uniqueness of Malassezia vespertilionis among its closest relatives may make it important in future research to better understand the evolution, life history, and pathogenicity of the Malassezia yeasts.

Persoonia - Molecular Phylogeny and Evolution of F↗

USGS2004

Mono Lake excursion recorded in sediment of the Santa Clara Valley, California

Two intervals recording anomalous paleomagnetic inclinations were encountered in the top 40 meters of research drill hole CCOC in the Santa Clara Valley, California. The younger of these two intervals has an age of 28,090 ± 330 radiocarbon years B.P. (calibrated age ∼32.8 ka). This age is in excellent agreement with the latest estimate for the Mono Lake excursion at the type locality and confirms that the excursion has been recorded by sediment in the San Francisco Bay region. The age of an anomalous inclination change below the Mono Lake excursion was not directly determined, but estimates of sedimentation rates indicate that the geomagnetic behavior it represents most likely occurred during the Mono Lake/Laschamp time interval (∼45–28 ka). If true, it may represent one of several recurring fluctuations of magnetic inclination during an interval of a weak geomagnetic dipole, behavior noted in other studies in the region.

California↗

USGS2012

Use of ASTER and MODIS thermal infrared data to quantify heat flow and hydrothermal change at Yellowstone National Park

The overarching aim of this study was to use satellite thermal infrared (TIR) remote sensing to monitor geothermal activity within the Yellowstone geothermal area to meet the missions of both the U.S. Geological Survey and the Yellowstone National Park Geology Program. Specific goals were to: 1) address the challenges of monitoring the surface thermal characteristics of the > 10,000 spatially and temporally dynamic thermal features in the Park (including hot springs, pools, geysers, fumaroles, and mud pots) that are spread out over ~ 5000 km 2 , by using satellite TIR remote sensing tools (e.g., ASTER and MODIS), 2) to estimate the radiant geothermal heat flux (GHF) for Yellowstone's thermal areas, and 3) to identify normal, background thermal changes so that significant, abnormal changes can be recognized, should they ever occur (e.g., changes related to tectonic, hydrothermal, impending volcanic processes, or human activities, such as nearby geothermal development). ASTER TIR data (90-m pixels) were used to estimate the radiant GHF from all of Yellowstone's thermal features and update maps of thermal areas. MODIS TIR data (1-km pixels) were used to record background thermal radiance variations from March 2000 through December 2010 and establish thermal change detection limits. A lower limit for the radiant GHF estimated from ASTER TIR temperature data was established at ~ 2.0 GW, which is ~ 30–45% of the heat flux estimated through geochemical thermometry. Also, about 5 km 2 of thermal areas was added to the geodatabase of mapped thermal areas. A decade-long time-series of MODIS TIR radiance data was dominated by seasonal cycles. A background subtraction technique was used in an attempt to isolate variations due to geothermal changes. Several statistically significant perturbations were noted in the time-series from Norris Geyser Basin, however many of these did not correspond to documented thermal disturbances. This study provides concrete examples of the strengths and limitations of current satellite TIR monitoring of geothermal areas, highlighting some specific areas that can be improved. This work provides a framework for future satellite-based thermal monitoring at Yellowstone and other volcanic and geothermal systems.

Idaho, Montana, Oregon, Wyoming↗

USGS2006

delta 15N and non-carbonate delta 13C values for two petroleum source rock reference materials and a marine sediment reference material

Samples of United States Geological Survey (USGS) Certified Reference Materials USGS Devonian Ohio Shale (SDO-1), and USGS Eocene Green River Shale (SGR-1), and National Research Council Canada (NRCC) Certified Marine Sediment Reference Material (PACS-2), were sent for analysis to four separate analytical laboratories as blind controls for organic rich sedimentary rock samples being analyzed from the Red Dog mine area in Alaska. The samples were analyzed for stable isotopes of carbon (delta13Cncc) and nitrogen (delta15N), percent non-carbonate carbon (Wt % Cncc) and percent nitrogen (Wt % N). SDO-1, collected from the Huron Member of the Ohio Shale, near Morehead, Kentucky, and SGR-1, collected from the Mahogany zone of the Green River Formation are petroleum source rocks used as reference materials for chemical analyses of sedimentary rocks. PACS-2 is modern marine sediment collected from the Esquimalt, British Columbia harbor. The results presented in this study are, with the exceptions noted below, the first published for these reference materials. There are published information values for the elemental concentrations of 'organic' carbon (Wt % Corg measured range is 8.98 - 10.4) and nitrogen (Wt % Ntot 0.347 with SD 0.043) only for SDO-1. The suggested values presented here should be considered 'information values' as defined by the NRCC Institute for National Measurement Reference Materials and should be useful for the analysis of 13C, 15N, C and N in organic material in sedimentary rocks.

Open-File Report↗

USGS2014

Robust, low-cost data loggers for stream temperature, flow intermittency, and relative conductivity monitoring

Water temperature and streamflow intermittency are critical parameters influencing aquatic ecosystem health. Low-cost temperature loggers have made continuous water temperature monitoring relatively simple but determining streamflow timing and intermittency using temperature data alone requires significant and subjective data interpretation. Electrical resistance (ER) sensors have recently been developed to overcome the major limitations of temperature-based methods for the assessment of streamflow intermittency. This technical note introduces the STIC (Stream Temperature, Intermittency, and Conductivity logger); a robust, low-cost, simple to build instrument that provides long-duration, high-resolution monitoring of both relative conductivity (RC) and temperature. Simultaneously collected temperature and RC data provide unambiguous water temperature and streamflow intermittency information that is crucial for monitoring aquatic ecosystem health and assessing regulatory compliance. With proper calibration, the STIC relative conductivity data can be used to monitor specific conductivity.

Water Resources Research↗

USGS2007

River plume patterns and dynamics within the Southern California Bight

Stormwater river plumes are important vectors of marine contaminants and pathogens in the Southern California Bight. Here we report the results of a multi-institution investigation of the river plumes across eight major river systems of southern California. We use in situ water samples from multi-day cruises in combination with MODIS satellite remote sensing, buoy meteorological observations, drifters, and HF radar current measurements to evaluate the dispersal patterns and dynamics of the freshwater plumes. River discharge was exceptionally episodic, and the majority of storm discharge occurred in a few hours. The combined plume observing techniques revealed that plumes commonly detach from the coast and turn to the left, which is the opposite direction of Coriolis influence. Although initial offshore velocity of the buoyant plumes was ∼50 cm/s and was influenced by river discharge inertia (i.e., the direct momentum of the river flux) and buoyancy, subsequent advection of the plumes was largely observed in an alongshore direction and dominated by local winds. Due to the multiple day upwelling wind conditions that commonly follow discharge events, plumes were observed to flow from their respective river mouths to down-coast waters at rates of 20–40 km/d. Lastly, we note that suspended-sediment concentration and beam-attenuation were poorly correlated with plume salinity across and within the sampled plumes (mean r 2 =0.12 and 0.25, respectively), while colored dissolved organic matter (CDOM) fluorescence was well correlated (mean r 2 =0.56), suggesting that CDOM may serve as a good tracer of the discharged freshwater in subsequent remote sensing and monitoring efforts of plumes.

California↗

USGS2015

National Earthquake Information Center systems overview and integration

Overview The primary mission of the U.S. Geological Survey (USGS) National Earthquake Information Center (NEIC) is comprehensive global earthquake monitoring (M4.5 or larger) and complete seismic monitoring of the United States for all significant earthquakes (M3.0 or larger or felt). In recent years, the NEIC has assumed a more prominent role in local and regional seismic monitoring, backup capabilities, and special studies. The NEIC uses numerous scientific algorithms to produce information products for local, national, and international disaster responders; infrastructure corporations (for example, utility providers and insurers); U.S. embassies worldwide; news agencies; national and international earth science research communities; and millions of public users. To support this mission, the NEIC operates a constant service to derive numerous information products as rapidly and accurately as possible. Derived information products and data sets provide a wealth of related earthquake information and include products such as location, magnitude, maps of shaking intensity, moment tensors, phase arrivals, and loss and damage reports. These information products are automatically derived in near real-time and refined over time. Earthquake information products vary from well formatted text files, to binary files, to directories full of many files. A team of analysts, who provide constant coverage, 24 hours a day, seven days a week, 365 days a year, review the acquired data and automatically derived products (1) in near real-time for rapid release and (2) in the weeks following events to produce historical catalogs of global earthquakes in support of scientific research. Data and products are reviewed both in near real-time for rapid release and in the weeks following events to produce historical catalogs of global earthquakes in support of scientific research. At the present time, the NEIC publishes data for approximately 25,000 earthquakes annually. The NEIC software system that supports this service and mission comprises many subsystems. The internals of each subsystem are often complex, but the integration between all of the subsystems is well defined, standardized, and straightforward. This Open-File Report describes the systems’ integration infrastructure and how all the subsystems fit together. It provides summary views of the system as a whole, along with detailed views of the inputs, outputs, and dependencies for each subsystem. It also outlines the many shared data services that support these subsystems and applications as well as external customers. The NEIC systems-integration effort is ongoing. Additional integration steps, needed to reach our goals, are discussed. It is important to note that this document provides a brief introduction to the work of dozens of software developers and IT specialists, spanning in many cases more than a decade. References to significant amounts of supporting documentation, code, and information are supplied within.

Open-File Report↗

USGS2017

Comparing efficiency of American Fisheries Society standard snorkeling techniques to environmental DNA sampling techniques

Analysis of environmental DNA (eDNA) is an emerging technique used to detect aquatic species through water sampling and the extraction of biological material for amplification. Our study compared the efficacy of eDNA methodology to American Fisheries Society (AFS) standard snorkeling surveys with regard to detecting the presence of rare fish species. Knowing which method is more efficient at detecting target species will help managers to determine the best way to sample when both traditional sampling methods and eDNA sampling are available. Our study site included three Navajo Nation streams that contained Navajo Nation Genetic Subunit Bluehead Suckers Catostomus discobolus and Zuni Bluehead Suckers C. discobolus yarrowi . We first divided the entire wetted area of streams into consecutive 100-m reaches and then systematically selected 10 reaches/stream for snorkel and eDNA surveys. Surface water samples were taken in 10-m sections within each 100-m reach, while fish presence was noted via snorkeling in each 10-m section. Quantitative PCR was run on each individual water sample in quadruplicate to test for the presence or absence of the target species. With eDNA sampling techniques, we were able to positively detect both species in two out of the three streams. Snorkeling resulted in positive detection of both species in all three streams. In streams where the target species were detected with eDNA sampling, snorkeling detected fish at 11–29 sites/stream, whereas eDNA detected fish at 3–12 sites/stream. Our results suggest that AFS standard snorkeling is more effective than eDNA for detecting target fish species. To improve our eDNA procedures, the amount of water collected and tested should be increased. Additionally, filtering water on-site may improve eDNA techniques for detecting fish. Future research should focus on standardization of eDNA sampling to provide a widely operational sampling tool.

Arizona, New Mexico↗

USGS2015

Chemicals of emerging concern in water and bottom sediment in the Great Lakes Basin, 2012: collection methods, analytical methods, quality assurance, and study data

In synoptic surveys of surface-water quality across the United States, a large group of organic chemicals associated with agricultural, household, and industrial waste have been detected. These chemicals are referred to collectively as chemicals of emerging concern (CECs) and include prescription drugs and antibiotics, over-the-counter medications, reproductive hormones, personal-care products, detergent metabolites, and flame retardants. The U.S. Geological Survey (USGS) collaborated with the U.S. Fish and Wildlife Service and the U.S. Environmental Protection Agency on a study to identify the presence of CECs in water and bottom-sediment samples collected during 2012 at 66 sites throughout the Great Lakes Basin. The 2012 effort is part of a long-term study that was initiated in 2010. The purposes of this report are to document the collection and analytical methods, provide the quality-assurance data and analyses, and provide the water and bottom-sediment data for this study of CECs in the Great Lakes Basin for 2012. A previous report documents data collected during 2010 and 2011. The methods used for chemical analyses were identical between the 2010–11 and 2012 studies, with the exception that a method to determine nontarget chemicals was used during 2010–11. The data from this study are published as a USGS Data Series Report to ensure adequate documentation of the original methods and provide a citable source for study data. This report contains no interpretations of the study data. The chemical data are as reported by the laboratory and have not been censored or adjusted unless otherwise noted. Field measurements were recorded and samples were collected in April and May and in September 2012, by U.S. Geological Survey, U.S. Fish and Wildlife Service, and U.S. Environmental Protection Agency personnel. Study sites included tributaries to the Great Lakes located near Duluth, Minnesota; King, Wisconsin; Green Bay, Wis.; Detroit, Michigan; Monroe, Mich.; Toledo, Ohio, and Rochester, New York. Water and bottom-sediment samples were analyzed at the USGS National Water Quality Laboratory in Denver, Colorado, for a broad suite of CECs. During this 2012 study, 140 environmental and 8 field duplicate samples of surface water and wastewater effluent, 1 field blank water sample, and 5 field spike water samples were collected or prepared. Water samples were analyzed at the USGS National Water Quality Laboratory using laboratory schedule 4433 for wastewater indicators, research method 8244 for pharmaceuticals, and laboratory schedule 4434 for steroid hormones, sterols, and bisphenol A. For wastewater indicators in unfiltered water, 61 of the 68 chemicals analyzed using laboratory schedule 4433 had detectable concentrations ranging from 0.002 to 64.4 micrograms per liter. Thirty-eight of the 48 chemicals analyzed using research method 8244 for pharmaceuticals in unfiltered water had detectable concentrations ranging from 0.002 to 3.32 micrograms per liter. Twelve of the 20 chemicals analyzed using laboratory schedule 4434 for steroid hormones, sterols, and bisphenol A in unfiltered water had detectable concentrations ranging from 0.43 to 120,000 nanograms per liter. During this study, 53 environmental samples, 4 field duplicate samples, and 8 field spike samples of bottom sediment and laboratory matrix-spike samples were analyzed for a wide variety of CECs at the USGS National Water Quality Laboratory using laboratory schedule 5433 for wastewater indicators; research method 6434 for steroid hormones, sterols, and bisphenol A; and research method 9008 for human-use pharmaceuticals and antidepressants. Forty of the 57 chemicals analyzed using laboratory schedule 5433 had detectable concentrations ranging from 1 to 49,000 micrograms per kilogram. Fourteen of the 20 chemicals analyzed using research method 6434 had detectable concentrations ranging from 0.04 to 24,940 nanograms per gram. Ten of the 20 chemicals analyzed using research method 9008 had detectable concentrations ranging from 0.59 to 197.5 micrograms per kilogram. Five of the 11 chemicals analyzed using research method 9008 had detectable concentrations ranging from 1.16 to 25.0 micrograms per kilogram.

Great Lakes Basin↗

USGS2024

Age, growth, and reproductive biology of Achilles tang (Acanthurus achilles) around Hawai'i Island, USA

A culturally important food fish in Hawai'i, Achilles tang ( Acanthurus achilles Shaw), recently experienced sharp declines in biomass, density, and average length in west Hawai'i Island. In December 2022, State resource managers placed a 2-year moratorium on the Achilles tang fishery in west Hawai'i Island so that the cause of the collapse could be explored. The lack of life-history information for Achilles tang from Hawai'i, or elsewhere in its range, has been noted as an impediment to decision-making. Therefore, our objectives were to characterize this population’s age, growth, and reproductive biology. In working with community fishers, we collected 363 individuals ranging 69 – 264 mm in fork length (FL) and 0 – 39 years in age based on estimates from otoliths. Achilles tang in Hawai'i exhibit a high growth rate, reaching 138 ± 11 mm FL (mean ± SE) in their first year, and exhibit relatively little growth after their second year (< 5 mm yr −1 ). The majority of males (92.0% of n = 101) and females (73.6% of n = 159) were classified as spawning capable or actively spawning, without annual or lunar periodicity. Our study highlights that the basic biology of Achilles tang poses unique challenges to fisheries managers looking to ensure its sustainable harvest. Future research will aim to characterize the susceptibility of recruits’ habitat to local environmental stressors and the relative connectivity of juvenile and adult habitats.

Hawaii↗

USGS2011

Sensing disease and danger: A survey of vertebrate PRRs and their origins

A key facet of the innate immune response lays in its ability to recognize and respond to invading microorganisms and cellular disturbances. Through the use of germ-line encoded PRRs, the innate immune system is capable of detecting invariant pathogen motifs termed pathogen-associated molecular patterns (PAMPS) that are distinct from host encoded proteins or products released from dying cells, which are known as damage-associated molecular patterns (DAMPs). PAMPs and DAMPs include both protein and nucleic acids for the detection and response to pathogens and metabolic "danger" signals. This is by far one of the most active areas of research as recent studies have shown retinoic acid inducible gene 1 (RIG1)-like receptors (RLRs), the nucleotide-binding domain, leucine-rich repeat containing proteins (NLRs) and Toll-like receptors (TLRs) and the recently described AIM-like receptors (ALRs) are responsible for initiating interferon production or the assembly and activation of the inflammasome, ultimately resulting in the release of bioactive IL-1 family members. Overall, the vertebrate PRR recognition machinery consists of seven domains (e.g., Death, NACHT, CARD, TIR, LRR, PYD, helicase), most of which can be traced to the very origins of the deuterostomes. This review is intended to provide an overview of the basic components that are used by vertebrates to detect and respond to pathogens, with an emphasis on these receptors in fish as well as a brief note on their likely origins.

Developmental and Comparative Immunology↗

USGS2002

Dynamic interpretation of slug tests in highly permeable aquifers

Considerable progress has been made in developing a theoretical framework for modeling slug test responses in formations with high hydraulic conductivity K . However, several questions of practical significance remain unresolved. Given the rapid and often oscillatory nature of test responses, the traditional hydrostatic relationship between the water level and the transducer‐measured head in the water column may not be appropriate. A general dynamic interpretation is proposed that describes the relationship between water level response and transducer‐measured head. This theory is utilized to develop a procedure for transforming model‐generated water level responses to transducer readings. The magnitude of the difference between the actual water level position and the apparent position based on the transducer measurement is a function of the acceleration and velocity of the water column, test geometry, and depth of the transducer. The dynamic approach explains the entire slug test response, including the often‐noted discrepancy between the actual initial water level displacement and that measured by a transducer in the water column. Failure to use this approach can lead to a significant underestimation of K when the transducer is a considerable distance below the static water level. Previous investigators have noted a dependence of test responses on the magnitude of the initial water level displacement and have developed various approximate methods for analyzing such data. These methods are re‐examined and their limitations clarified. Practical field guidelines are proposed on the basis of findings of this work. The soundness of the dynamic approach is demonstrated through a comparison of K profiles from a series of multilevel slug tests with those from dipole‐flow tests performed in the same wells.

Water Resources Research↗

USGS2000

Construction, completion, and testing of replacement monitoring wells MW 3-2, MW 6-2, MW 7-2, and MW 11-2, Mountain Home Air Force Base, Idaho, February through April 2000

In February and March 2000, the U.S. Geological Survey Western Regional Research Drilling Operation constructed replacement monitoring wells MW 3–2, MW 6–2, MW 7–2, and MW 11–2 as part of a regional ground-water monitor- ing network for the Mountain Home Air Force Base, Elmore County, Idaho. Total well depths ranged from 435.5 to 456.5 feet, and initial depth-to-water measurements ranged from about 350 to 375 feet below land surface. After completion, wells were pumped and onsite measurements were made of water temperature, specific conductance, pH, and dissolved oxygen. At each well, natural gamma, spontaneous potential, resistivity, caliper, and temperature logs were obtained from instruments placed in open boreholes. A three- dimensional borehole flow analysis was completed for MW 3–2 and MW 11–2, and a video log was obtained for MW 11–2 to annotate lithology and note wet zones in the borehole above saturated rock.

Idaho↗

USGS2010

Proceedings of the U.S. Geological Survey Interdisciplinary Microbiology Workshop, Estes Park, Colorado, October 15-17, 2008

Preface A U.S. Geological Survey Interdisciplinary Microbiology Workshop was held in Estes Park, Colorado, on October 15-17, 2008. Participants came from all USGS regions and disciplines. This report contains abstracts from 36 presentations and 35 poster sessions and notes from 5 breakout sessions. The seven presentation topics follow: Ecology of wildlife and fish disease Mechanisms of fish and wildlife disease Microbial ecology Geographic patterns/visualization Public health and water quality Geomicrobiology Ecosystem function The six poster session topics follow: Wildlife disease Disease detection methods Water quality Microbial ecology Metabolic processes Tools and techniques Five working groups met in breakout sessions on October 16, 2008. The highlights for each working group are summarized in this report, and their goals are listed below: Working Group I: to plan a Fact Sheet on interdisciplinary microbiology in the USGS Working Group II: to plan a USGS interdisciplinary microbiology Web site Working Group III: to suggest ways to broadcast and publicize the types of microbiology conducted at the USGS Working Group IV: to identify emerging issues in USGS interdisciplinary microbiology research Working Group V: to identify potential opportunities for interdisciplinary microbiology work at the USGS After the workshop, the USGS interdisciplinary microbiology Web site was activated in June 2009 at http://microbiology.usgs.gov/.

Scientific Investigations Report↗

USGS1974

Distribution of copper in biotite and biotite alteration products in intrusive rocks near two Arizona porphyry copper deposits

Biotite and its alteration products (primarily chlorite) from igneous rocks around the Ray and Esperanza (Esperanza-Sierrita) porphyry copper deposits, Arizona, were analyzed for copper by electron microprobe. The copper occurs in amounts >90 p/m (limit of detection) in most of the chlorites analyzed, is concentrated at the optical and chemical boundary of chlorite and biotite, and is not associated with sulfur. Most unaltered igneous and hydrothermal biotites analyzed contain <90 p/m Cu, and except for one sample, all copper that was detected can be explained as contamination by copper from chlorite grains. The paucity of detectable copper in igneous and hydrothermal biotite and its presence in daughter chlorite suggest that the positive association noted by some workers between the proximity of an ore deposit and the copper content of biotite might be partly the result: of increased amounts of chloritization of biotite near a deposit coupled with difficulty in physically cleaning the biotite separates. Additionally, previous speculations that (1) part of the copper in a deposit may come from altered biotite, and (2) copper in biotite indicates how copper behaves in a differentiating magma, are of doubtful value if based on data derived from analyses of bulk mineral separates.

Arizona↗

USGS2007

Deep-water chaunacid and lophiid anglerfishes (Pisces: Lophiiformes) off the Southeastern United States

Recent research cruises to deep (80–910 m) reef habitats off the south-eastern U.S. and in the northern Gulf of Mexico have provided new information on the diagnostic characteristics, behaviours, colour patterns in life, bottom associations, distributions and maximum sizes of species of the anglerfish genera Chaunax , Lophiodes and Sladenia . Chaunax stigmaeus occurred much further south than previously known (Blake Plateau off South Carolina), and all C. stigmaeus observed were found associated with dense beds of dead coral ( Lophelia pertusa ) rubble or on broken hard bottom. In contrast, Chaunax suttkusi was found on soft bottoms. Chaunax stigmaeus and C. suttkusi appear to be sympatric over a major portion of their ranges. Because knowledge of pigmentation in live or freshly caught Chaunax is critical to distinguish some members of the genus, changes in the colouration of C. suttkusi were noted and documented photographically immediately after death and after fixation. The yellow spots found on some, but not all specimens, temporarily disappeared completely after death, but they reappeared after fixation, slowly disappearing thereafter along with other carotenoid pigments. Lophiodes beroe and Lophiodes monodi were collected for the first time off the Atlantic coast of the U.S., being previously known only from the Gulf of Mexico, Caribbean Sea and the northern coast of South America. For both species ( L. beroe and L. monodi ), the collections included the two largest known representatives of the species (400 and 325 mm standard length, respectively). Lophiodes beroe commonly occurred on L. pertusa rubble, and seemed to prefer this habitat. Occupying such a habitat that is deep and difficult to sample probably explains how this common species escaped detection. Only a single L. monodi was collected or observed, so this species appears to be uncommon in this geographic area or at least so on coral rubble habitat. Detailed aspects of the colour patterns of both species were noted. In particular, L. beroe displayed a characteristic pattern of white patches in life that were not apparent after death. The first photographic documentation of the colour pattern in life and of the pharyngeal pigmentation of Lophiodes reticulatus is provided. The third known specimen of Sladenia shaefersi , and the first to be taken in U.S. waters was collected from coral rubble near the base of a steep 200 m scarp on the Blake Plateau.

Gulf of Mexico↗

USGS2002

Challenges in observational seismology

Earthquake seismology became a quantitative scientific discipline after instruments were developed to record seismic waves in the late 19th century ( Dewey and Byerly, 1969 ; Chapter 1 by Agnew). Earthquake seismology is essentially based on field observations. The great progress made in the past several decades was primarily due to increasingly plentiful and high-quality data that are readily distributed. Our ability to collect, process, and analyze earthquake data has been accelerated by advances in electronics, communications, computers, and software (see Chapter 85 edited by Snoke and Garcia-Fernandez). Instrumental observation of earthquakes has been carried out for a little over 100 years by seismic stations and networks of various sizes, from local to global scales (see Chapter 87 edited by Lahr and van Eck). The observed data have been used, for example, (1) to compute the source parameters of earthquakes, (2) to determine the physical properties of the Earth's interior, (3) to test the theory of plate tectonics , (4) to map active faults, (5) to infer the nature of damaging ground shaking, and (6) to carry out seismic hazard analysis. Construction of a satisfactory theory of the earthquake process has not yet been achieved within the context of physical laws. Good progress, however, has been made in building a physical foundation of the earthquake source process, partly as a result of research directed toward earthquake prediction. This chapter is intended for a general audience. Technical details are not given, but relevant references and chapters in this Handbook are referred to. The first part of this chapter presents a brief overview of the observational aspects of earthquake seismology, concentrating on instrumental observations of seismic waves generated by earthquakes (i.e., seismic monitoring), and readers are referred to Chapter 49 by Musson and Cecic for noninstrumental observations. A few key developments and practices are summarized by taking a general view, since many national and regional developments have been chronicled in national and institutional reports (see Chapter 79 edited by Kisslinger). In the latter part of this chapter, the nature of seismic monitoring and some challenges in observational seismology are discussed from a personal perspective. Comments of a technical or philosophical nature are given in the Notes at the end of the chapter, and they are referenced by superscript numbers in the text.

International Geophysics↗

USGS2004

Map showing geology, oil and gas fields, and geologic provinces of the Caribbean Region

This CD-ROM compilation contains a map and associated spatial data showing surface geology, faults, oil and gas field centerpoints, and geologic provinces of the Caribbean region, draped over a shaded relief image of topography and bathymetry. The map is provided in the Environmental Systems Research Institute, Inc. (ESRI) ArcMap and ArcReader GIS formats, as well as in Adobe Acrobat Portable Document Format (PDF). On this CD-ROM, ESRI ArcReader and Adobe Acrobat Reader software provide a way to view and interact with the maps. The organization and user-friendly navigation of this CD-ROM ensure easy access to its maps and data by using the links on the right side of each page. A link to the USGS World Energy Project website is also provided to access the latest information, updates, and interactive maps, as they relate to this and other world energy products. In addition, system requirements, permission, and contact information can be found in the readme section of this product. Navigation of this product can be fully utilized with most web browsers (Internet Explorer 6.0/Netscape Navigator 7.1 or later recommended). Note: an Internet connection is necessary to view USGS website links, World Energy website, and the Caribbean Internet Map Service.

Open-File Report↗