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At least 271 records · Page 15Linked to original sources
USGS2002

Emsian synorogenic paleogeography of the Maine Applachians

The Acadian deformation front in the northern Appalachians of Maine and New Hampshire can now be closely located during the early Emsian (Early Devonian; 408-406 Ma). Tight correlations between paleontologically and isotopically dated rocks are possible only because of a new 408-Ma time scale tie point for the early Emsian. The deformation front lay between a belt of Lower Devonian flysch and molasse that were deposited in an Acadian foreland basin and had not yet been folded and a belt of early Emsian plutons that intruded folded Lower Devonian rocks. This plutonic belt includes the newly dated Ore Mountain gabbro (U/Pb; 406 Ma), which hosts magmatic-sulfide mineralization. Along the deformation front, a 407-Ma pluton that locally truncates Acadian folds (Katahdin) was the feeder to volcanic rocks (Traveler Rhyolite; 406-407 Ma) that are part of the foreland-basin succession involved in these same folds. The Emsian igneous rocks thus define a syncollisional magmatic province that straddled the deformation front. These findings bear on three alternative subduction geometries for the Acadian collision.

Journal of Geology↗
USGS2025

Identical sequence types of Yersinia ruckeri associated with lethal disease in wild-caught invasive Blue Catfish and cultured hybrid catfish (Channel Catfish ♀ × Blue Catfish ♂) from disparate aquatic ecosystems

Objective The Blue Catfish Ictalurus furcatus is commonly raised in warmwater aquaculture in the United States to produce Channel Catfish I. punctatus × Blue Catfish hybrids. It is also a prominent aquatic invasive species of concern in the mid-Atlantic region of the United States. Here, Yersina ruckeri was isolated from moribund Blue Catfish and hybrid catfish from disparate regions of the USA. The goal of the research here was to compare these Y. ruckeri strains to each other and other known strains for which adequate sequence data was available. In addition, we sought to determine if the strain from Blue Catfish was pathogenic to Rainbow Trout Oncorhynchus mykiss . Methods Moribund hybrid catfish from culture ponds in Mississippi were processed for diagnostic evaluation in March 2016. In April 2022, a moribund Blue Catfish specimen was collected from a tributary of the Nanticoke River in Maryland. Bacterial isolates were identified and characterized using biochemical tests, antimicrobial sensitivity screening, serotyping, and complete or partial genome sequencing. Disease pathology was described via histology. The isolate from Blue Catfish was used in challenge experiments to determine if it was pathogenic to Rainbow Trout. Multilocus sequencing typing was conducted using the PubMLST database. Results Biochemical testing was consistent with Y. ruckeri . A draft genome of the Y. ruckeri isolate was assembled based on Oxford Nanopore Technology sequencing and identified a single genomic replicon (3,791,418 bp) consistent in size to other Y. ruckeri genomes and a pLT plasmid (60, 933 bp). The challenge study demonstrated no significant virulence of this isolate for Rainbow Trout ( Y. ruckeri ). This isolate was most similar to other strains isolated from ictalurids. Notably, the gyrase B gene from this isolate was identical to that of archived strains isolated from moribund Mississippi hybrid catfish aquaculture during 2016 and these isolates share identical PubMLST sequence type profiles. Similarly, they shared a pLT plasmid that differed by only 6 bp. This plasmid has never been reported from trout isolates and appears to be unique to ictalurids. Conclusions Analyses here provide preliminary genetic evidence that geographically distant (Maryland and Mississippi, USA) isolates of Y. ruckeri from ictalurids are genetically similar to each other and Y. ruckeri (strain SC09) that infects ictalurids in China. This strain is not a biothreat to Rainbow Trout at typical culture temperatures.

Journal of Aquatic Animal Health↗
USGS2022

Using surrogate insects in acid bioassays for development of new controls for Varroa destructor (Arachnida: Varroidae)

Resistance to traditional synthetic compounds by Varroa destructor Anderson and Trueman and shortcomings of the organic acid class of acaracides commonly used in varroa management requires continual development of new controls. V. destructor , however, are difficult to obtain for use in control bioassays because they are obligate parasites that cannot be easily reared outside of a honey bee colony. We conducted bioassays using other, more easily obtainable species to find organisms that could be used as surrogates for V. destructor when testing new potential controls. We compared the toxicities of acetic acid, lactic acid, formic acid, and oxalic acid at 0.005%, 0.05%, 0.5%, 5%, and 50% (20% oxalic acid only) concentrations based on natural volatility (nonheated) for the control of two beetle species, Oryzaephilus surinamensis L. and Alphitobius diaperinus Panzer, greater wax moth larvae, Galleria mellonella L., and V. destructor . The assay results were consistent across all species with formic acid and acetic acid showing 100% mortality of all four test species at 50% concentration. The assays also provided insight into the method of application (vaporization or contact) needed to cause mortality. Our results show that other organisms can be used in place of V. destructor for initial testing of acids and possibly other chemicals for control of the ectoparasite.

Journal of Economic Entomology↗
USGS2022

Give and take: Effects of genetic admixture on mutation load in endangered Florida panthers

Genetic admixture is a biological event inherent to genetic rescue programs aimed at the long-term conservation of endangered wildlife. Although the success of such programs can be measured by the increase in genetic diversity and fitness of subsequent admixed individuals, predictions supporting admixture costs to fitness due to the introduction of novel deleterious alleles are necessary. Here, we analyzed nonsynonymous variation from conserved genes to quantify and compare levels of mutation load (i.e. proportion of deleterious alleles and genotypes carrying these alleles) among endangered Florida panthers and non-endangered Texas pumas. Specifically, we used canonical (i.e. non-admixed) Florida panthers, Texas pumas, and F 1 (canonical Florida × Texas) panthers dating from a genetic rescue program and Everglades National Park panthers with Central American ancestry resulting from an earlier admixture event. We found neither genetic drift nor selection significantly reduced overall proportions of deleterious alleles in the severely bottlenecked canonical Florida panthers. Nevertheless, the deleterious alleles identified were distributed into a disproportionately high number of homozygous genotypes due to close inbreeding in this group. Conversely, admixed Florida panthers (either with Texas or Central American ancestry) presented reduced levels of homozygous genotypes carrying deleterious alleles but increased levels of heterozygous genotypes carrying these variants relative to canonical Florida panthers. Although admixture is likely to alleviate the load of standing deleterious variation present in homozygous genotypes, our results suggest that introduced novel deleterious alleles (temporarily present in heterozygous state) in genetically rescued populations could potentially be expressed in subsequent generations if their effective sizes remain small.

Journal of Heredity↗
USGS2018

Hatchery strain contributions to emerging wild lake trout populations in Lake Huron

Recent assessments indicate the emergence of naturally produced lake trout ( Salvelinus namaycush ) recruitment throughout Lake Huron in the North American Laurentian Great Lakes (>50% of fish <7 years). Because naturally produced fish derived from different stocked hatchery strains are unmarked, managers cannot distinguish strains contributing to natural recruitment. We used 15 microsatellite loci to identify strains of naturally produced lake trout ( N = 1567) collected in assessment fisheries during early (2002–2004) and late (2009–2012) sampling periods. Individuals from 13 American and Canadian hatchery strains ( N = 1143) were genotyped to develop standardized baseline information. Strain contributions were estimated using a Bayesian inferential approach. Deviance information criteria were used to compare models evaluating strain contributions at different spatial and temporal scales. The best performing models were the most complex models, suggesting that hatchery strain contributions to naturally produced lake trout varied spatially among management districts and temporally between time periods. Contributions of Seneca strain lake trout were consistently high across most management districts, with contributions increasing from early to late time periods (estimates ranged from 52% to 94% for the late period across 8 of 9 districts). Strain contributions deviated from expectations based on historical stocking levels, indicating strains differed with respect to survival, reproductive success, and/or dispersal. Knowledge of recruitment levels of strains stocked in different management districts, and how strain-specific recruitment varies temporally, spatially, and as a function of local or regional stocking is important to prioritize strains for future stocking and management of the transition process from primarily hatchery to naturally produced stocks.

Journal of Heredity↗
USGS2016

Reproduction in moose at their southern range limit

Reproduction is a critical fitness component in large herbivores. Biogeographic models predict that populations occurring at the edges of the range may have compromised reproductive rates because of inferior habitat at range peripheries. When reproductive rates are chronically low, ungulate populations may lack the resiliency to rebound quickly after periods of environmental stress, and this effect may be greatest for heat-sensitive organisms at their southern range limit. To assess the demographic vulnerability of moose ( Alces alces ), we studied relationships between reproductive rates, maternal age, and rump fat in the southernmost naturally occurring moose population in North America. For prime-aged moose in our study, pregnancy rates were high (92%), but moose aged < 3 or > 9 years had low pregnancy rates (32% and 38%, respectively). The relationship between rump fat and pregnancy was nonlinear such that a threshold of at least 2mm of rump fat yielded a high probability of being pregnant midwinter. In contrast, among pregnant moose, the probability of both producing a calf and recruiting it until spring increased linearly with rump fat. We also conducted a meta-analysis of pregnancy and twinning rates for adult (≥ 2 years) moose across a latitudinal gradient to compare reproductive rates from our study to other populations in North America. Moose living at southern latitudes tended to have lower reproductive rates than those living in the core of moose range, implying that southern moose populations may be demographically more vulnerable than northern moose populations.

Utah↗
USGS2016

Dog days of summer: Influences on decision of wolves to move pups

For animals that forage widely, protecting young from predation can span relatively long time periods due to the inability of young to travel with and be protected by their parents. Moving relatively immobile young to improve access to important resources, limit detection of concentrated scent by predators, and decrease infestations by ectoparasites can be advantageous. Moving young, however, can also expose them to increased mortality risks (e.g., accidents, getting lost, predation). For group-living animals that live in variable environments and care for young over extended time periods, the influence of biotic factors (e.g., group size, predation risk) and abiotic factors (e.g., temperature and precipitation) on the decision to move young is unknown. We used data from 25 satellite-collared wolves ( Canis lupus ) in Idaho, Montana, and Yellowstone National Park to evaluate how these factors could influence the decision to move pups during the pup-rearing season. We hypothesized that litter size, the number of adults in a group, and perceived predation risk would positively affect the number of times gray wolves moved pups. We further hypothesized that wolves would move their pups more often when it was hot and dry to ensure sufficient access to water. Contrary to our hypothesis, monthly temperature above the 30-year average was negatively related to the number of times wolves moved their pups. Monthly precipitation above the 30-year average, however, was positively related to the amount of time wolves spent at pup-rearing sites after leaving the natal den. We found little relationship between risk of predation (by grizzly bears, humans, or conspecifics) or group and litter sizes and number of times wolves moved their pups. Our findings suggest that abiotic factors most strongly influence the decision of wolves to move pups, although responses to unpredictable biotic events (e.g., a predator encountering pups) cannot be ruled out.

Journal of Mammalogy↗
USGS1998

Reservoir competence of Microtus pennsylvanicus (Rodentia: Cricetidae) for the Lyme disease spirochete, Borrelia burgdorferi

The reservoir competence of the meadow vole, Microtus pennsylvanicus Ord, for the Lyme disease spirochete Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner was established on Patience Island, RI. Meadow voles were collected from 5 locations throughout Rhode Island. At 4 of the field sites, M. pennsylvanicus represented only 4.0% ( n = 141) of the animals captured. However, on Patience Island, M. pennsylvanicus was the sole small mammal collected ( n = 48). Of the larval Ixodes scapularis Say obtained from the meadow voles on Patience Island, 62% ( n = 78) was infected with B. burgdorferi . Meadow voles from all 5 locations were successfully infected with B. burgdorferi in the laboratory and were capable of passing the infection to xenodiagnostic I. scapularis larvae for 9 wk. We concluded that M. pennsylvanicus was physiologically capable of maintaining B. burgdorferi infection. However, in locations where Peromyscus leucopus (Rafinesque) is abundant, the role of M. pennsylvanicus as a primary reservoir for B. burgdorferi was reduced.

Journal of Medical Entomology↗
USGS1969

Rheology of basalt in the melting range

Experimental data have been obtained for viscosities of tholeiite melts at temperatures from 1300 to 1120 °Cat 1 atm, using a concentric cylinder viscometer. The apparent viscosity increases more than two orders of magnitude between 1200 and 1120 °C (0–25 per cent crystallization) for shear rates of about 10 sec -1 and even more for lower shear rates. Non-Newtonian behaviour of ‘pseudo-plastic type’ becomes extremely pronounced at temperatures below about 1130 °C. At these temperatures, differences of less than 5 °C can produce changes in apparent viscosity amounting to orders of magnitude. These observations have led to the conclusion that the heat of deformation must itself influence rheological behaviour in the melting range. An equation for thermal energy balances and their rates of change is constructed and placed in a non-dimensional form that has been given published solutions by I. J. Gruntfest (1963) relating the shear stress, rate of strain, and temperature through the temperature dependence of viscosity. The results show that in an adiabatic system the heating rate increases with time so that the temperature eventually runs out of bounds, a process termed ‘thermal feedback’ by Gruntfest. A hypothesis of shear melting is derived on the basis of a simplified viscosity function extrapolated to the solidus temperature. The hypothesis is applied to magma generation in the earth on the basis of dimensional arguments. It is also suggested that thermal instabilities give rise to a sort of viscous failure responsible for deep-focus earthquakes, and that the two phenomena have the same cause relating ultimately to a gravitational energy source.

Journal of Petrology↗
USGS1973

Oxidation during magmatic differentiation, Finnmarka Complex, Oslo area, Norway: Part 2, the mafic silicates

Electron-microprobe analyses are presented for pyroxene, amphibole, and biotite from monzonite, granodiorite, and granite at Finnmarka, Norway. Compositional trends measured in biotite, present in all three rock types, and in amphibole, present in the monzonite and granodiorite, are markedly atypical and are interpreted as reflecting crystallization under progressively more oxidizing conditions. The average Fe/Fe + Mg for biotites from successively more silicic rock types changes from 0.64 → 0.35 → 0.28, and for amphiboles changes from 0.58 in the monzonite to 0.29 in the granodiorite. Analyses of selected areas within amphibole grains in the granodiorite show marked chemical variations, although single-crystal X-ray photographs are sharp and do not reveal multiple phases. On the basis of 33 such analyses, four coupled substitutions are identified as operative; the most unusual finding is the relation of 1 Ti cation to 4 Al IV cations in the unit cell. Variations within individual amphibole grains of the granodiorite resemble changes noted in evolution of amphibole composition from monzonite to granodiorite and are interpreted as reflecting progressive oxidation. Consideration of these data for the mafic silicates, data for the opaque oxides, and the extensive formation of sphene in the granodiorite, has allowed development of schematic reactions and an overall picture of magmatic environment and evolution at Finnmarka. Crystallization apparently took place at PH 2 o of 1000 bars or less and a temperature of about 700 °C. The trend of oxidation during differentiation is more extreme than any heretofore reported. Amphiboles, as well as biotites, may participate in oxidation reactions and may reflect the oxidation-reduction processes that occurred during magmatic evolution.

Oslo↗
USGS1962

Stability relations of the ferruginous biotite, annite

Annite , KFe 3 AISi 3 O 10 (OH) 2 a member of the iron biotites and the ferrous analogue of phlogopite, has been synthesized and its phase relations have been determined as functions of temperature, fugacity of oxygen (fo 2 ), and total pressure (P total ≈PH 2 O+PH 2 ). A method for controlling fo 2 at high total pressures is described, and data for the 'oxygen buffers' used are given. Buffers range from quartz+iron+fayalite assemblages (low fo 2 ) to magnetite-hematite assemblages (high fo 2 ). Optical properties and unit-cell dimensions of synthetic annites depend on the conditions of synthesis.By recalculating published analyses of natural iron-rich biotites it can be shown that one cannot assume a constant hydrogen content for such biotites. Oxidation may have occurred by drying at 115°C. Octahedral occupancy therefore cannot be calculated from such data.Phase relations of annite are presented in 2,070 and 1,035 bar sections. Depending on fo 2 -T values annite was found to decompose to one of the following assemblages: hematite+ sanidine, magnetite+sanidine, fayalite+leucite+kalsilite, iron+sanidine. All decompositions are dehydration and redox reactions and are sensitive to changes in fH 2 0 and fo 2 (or fH 2 0 and fH 2 ). At 2, 070 bars total pressure annite +magnetite+sanidine can coexist between 425°C and 825° C, depending upon the magnitude of fo 2 .In the presence of quartz the stability field of annite is more restricted. Phase equilibria in the system KAlSiO 4 -SiO 2 -Fe-O 2 -H 2 have been summarized schematically.Wherever possible, thermodynamic extrapolations are made to test the internal consistency of the data. Enthalpies of formation are calculated for both annite and phlogopite. Ranges of fo 2 values in nature as well as mechanisms for changes in fo 2 are investigated. It is useful to distinguish between assemblages which are internally buffered with respect to fo 2 changes and those which are not buffered. The applications of individual reactions involving annite to specific geologic problems are discussed with respect to igneous, metamorphic, and sedimentary rocks.

Journal of Petrology↗
USGS1990

Calc-alkaline, shoshonitic, and primitive tholeiitic lavas from monogenetic volcanoes near Crater Lake, Oregon

Quaternary monogenetic volcanism in the High Cascades of Oregon is manifested by cinder cones, lava fields, and small shields. Near Crater Lake caldera, monogenetic lava compositions include: low-K (as low as 0·09% K 2 O) high-alumina olivine tholeiite (HAOT); medium-K. calc-alkaline basalt, basaltic andesite, and andesite; and shoshonitic basaltic andesite (2·1% K 2 O, 1750 ppm Sr at 54% SiO 2 ). Tholeiites have MORB-like trace element abundances except for elevated Sr, Ba, and Th and low high field strength elements (HFSE), and they represent near-primary liquids. They are similar to HAOTs from the Cascades and adjacent Basin and Range, and to many primitive basalts from intraoceanic arcs. Calc-alkaline lavas show a well-developed arc signature of high large-ion lithophile elements (LILE) and low HFSE. Their Zr and Hf concentrations are at least partly decoupled from those of Nb and Ta; HREE are low relative to HAOT. Incompatible element abundances and ratios vary widely among basaltic andesites. Some calc-alkaline lavas vented near Mount Mazama contain abundant gabbroic microxcnoliths, and are basaltic andesitic magmas contaminated with olivine gabbro. A calc-alkaline basalt and a few basaltic andesites have MgO and compatible trace element contents that suggest only minor fractionation. There appears to be a compositional continuum between primitive tholeiitic and calc-alkaline lavas. Compositional variation within suites of comagmatic primitive lavas, both tholeiitic and calc-alkaline, mainly results from different degrees of partial melting. Sources of calc-alkaline primary magmas were enriched in LILE and LREE by a subduction component and contained residual garnet, whereas sources of HAOTs had lower LILE and LREE concentrations and contained residual clinopyroxene. High and variable LILE and LREE contents of calc-alkaline lavas reflect variations in fluid-transported subduction component added to the mantle wedge, degree of partial melting, and possibly also interaction with rocks or partial melts in the lower crust. Andesites were derived from calc-alkaline basaltic andesites by fractionation of plagioclase+augite+magnetite+apatite ± orthopyroxene or olivine, commonly accompanied by assimilation. Many andesites are mixtures of andesitic or dacitic magma and a basaltic or basaltic andesitic component, or are contaminated with gabbroic material. Mingled basalt, andesite, and dacite of Williams Crater formed by multi-component, multi-stage mixing of basaltic andesitic magma, gabbro, and dacitic magma. The wide range of compositions vented from monogenetic volcanoes near Crater Lake is a result of the thick crust coupled with mild tectonic extension superimposed on a subduction-related magmatic arc.

Oregon↗
USGS1993

Pressure increases, the formation of chromite seams, and the development of the ultramafic series in the Stillwater Complex, Montana

This paper explores the hypothesis that chromite seams in the Stillwater Complex formed in response to periodic increases in total pressure in the chamber. Total pressure increased because of the positive δ V of nucleation of CO 2 bubbles in the melt and their subsequent rise through the magma chamber, during which the bubbles increased in volume by a factor of 4–6. By analogy with the pressure changes in the summit chambers of Kilauea and Krafla volcanoes, the maximum variation was 0⋅2–0⋅25 kbar, or 5–10% of the total pressure in the Stillwater chamber. An evaluation of the likelihood of fountaining and mixing of a new, primitive liquid that entered the chamber with the somewhat more evolved liquid already in the chamber is based upon calculations using observed and inferred velocities and flow rates of basaltic magmas moving through volcanic fissures. The calculations indicate that hot, dense magma would have oozed, rather than fountained into the chamber, and early mixing of the new and residual magmas that could have resulted in chromite crystallizing alone did not take place.

Journal of Petrology↗
USGS2000

Rhyolite themobarometry and the shallowing of the magma reservoir, Coso volcanic field, California

The compositionally bimodal Pleistocene Coso volcanic field is located at the western margin of the Basin and Range province ∼60 km north of the Garlock fault. Thirty-nine nearly aphyric high-silica rhyolite domes were emplaced in the past million years: one at 1 Ma from a transient magma reservoir, one at ∼0·6 Ma, and the rest since ∼0·3 Ma. Over the past 0·6 My, the depth from which the rhyolites erupted has decreased and their temperatures have become slightly higher. Pre-eruptive conditions of the rhyolite magmas, calculated from phenocryst compositions using the two-oxide thermometer and the Al-in-hornblende barometer, ranged from 740°C and 270 MPa (2·7 kbar; ∼10 km depth) for the ∼0·6 Ma magma, to 770°C and 140 MPa (1·4 kbar; ∼5·5 km) for the youngest (∼0·04 Ma) magma. Results are consistent with either a single rhyolitic reservoir moving upward through the crust, or a series of successively shallower reservoirs. As the reservoir has become closer to the surface, eruptions have become both more frequent and more voluminous.

California↗
USGS2001

Eruptive stratigraphy of the Tatara-San Pedro complex, 36°S, sourthern volcanic zone, Chilean Andes: Reconstruction method and implications for magma evolution at long-lived arc volcanic centers

The Quaternary Tatara–San Pedro volcanic complex (36°S, Chilean Andes) comprises eight or more unconformity-bound volcanic sequences, representing variably preserved erosional remnants of volcanic centers generated during ∼930 ky of activity. The internal eruptive histories of several dominantly mafic to intermediate sequences have been reconstructed, on the basis of correlations of whole-rock major and trace element chemistry of flows between multiple sampled sections, but with critical contributions from photogrammetric, geochronologic, and paleomagnetic data. Many groups of flows representing discrete eruptive events define internal variation trends that reflect extrusion of heterogeneous or rapidly evolving magma batches from conduit–reservoir systems in which open-system processes typically played a large role. Long-term progressive evolution trends are extremely rare and the magma compositions of successive eruptive events rarely lie on precisely the same differentiation trend, even where they have evolved from similar parent magmas by similar processes. These observations are not consistent with magma differentiation in large long-lived reservoirs, but they may be accommodated by diverse interactions between newly arrived magma inputs and multiple resident pockets of evolved magma and/or crystal mush residing in conduit-dominated subvolcanic reservoirs. Without constraints provided by the reconstructed stratigraphic relations, the framework for petrologic modeling would be far different. A well-established eruptive stratigraphy may provide independent constraints on the petrologic processes involved in magma evolution—simply on the basis of the specific order in which diverse, broadly cogenetic magmas have been erupted. The Tatara–San Pedro complex includes lavas ranging from primitive basalt to high-SiO 2 rhyolite, and although the dominant erupted magma type was basaltic andesite (∼52–55 wt % SiO 2 ) each sequence is characterized by unique proportions of mafic, intermediate, and silicic eruptive products. Intermediate lava compositions also record different evolution paths, both within and between sequences. No systematic long-term pattern is evident from comparisons at the level of sequences. The considerable diversity of mafic and evolved magmas of the Tatara–San Pedro complex bears on interpretations of regional geochemical trends. The variable role of open-system processes in shaping the compositions of evolved Tatara–San Pedro complex magmas, and even some basaltic magmas, leads to the conclusion that addressing problems such as arc magma genesis and elemental fluxes through subduction zones on the basis of averaged or regressed reconnaissance geochemical datasets is a tenuous exercise. Such compositional indices are highly instructive for identifying broad regional trends and first-order problems, but they should be used with extreme caution in attempts to quantify processes and magma sources, including crustal components, implicated in these trends.

Andes Mountains, Tatara-San Pedro Volcanic Complex↗
USGS2020

Petrologic insights into rift zone magmatic interactions from the 2011 eruption of Kīlauea Volcano, Hawaiʻi

The high frequency of historical eruptions at Kīlauea Volcano presents an exceptional opportunity to address fundamental questions related to the transport, storage, and interaction of magmas within rift zones. The Nāpau Crater area on Kīlauea’s East Rift Zone (ERZ) experienced nine fissure eruptions within 50 years (1961–2011). Most of the magma intruded during these frequent eruptions remained stored within the rift zone, creating a potential magma mixing depot within the ERZ. The superbly monitored and sampled 2011 eruption (Puʻu ʻŌʻō episode 59) presents an extraordinary opportunity to evaluate magma mixing processes within the ERZ. Whole-rock, glass, and olivine compositions were determined, not only for lava from the 2011 eruption, but also for a new suite of Nāpau Crater area samples from the 1963, 1965, 1968, 1983, and 1997 eruptions, as well as the previously undocumented 1922 eruption. Whole-rock XRF data revealed two geochemically distinct magma batches for episode 59: one less evolved (∼6·6 wt % MgO, 0·46 wt % K2O) than the other (∼6·2 wt % MgO, 0·58 wt % K2O). Episode 59 lava is remarkably aphyric (∼0·1 vol. % phenocrysts), making use of mineralogy to identify parent magma affinities problematic. Linear compositional trends of whole-rock major and trace elements, and reversely zoned olivine crystals indicate episode 59 lavas underwent magma mixing. Least squares regression calculations and plots of major and trace element data, were used to evaluate whether the episode 59 samples are products of mixing summit-derived magma with residual magma from previous Nāpau Crater area eruptions. The regression results and trace element ratios are inconsistent with previously proposed mixing scenarios, but they do support mixing between summit-derived magma and residual magma from the 1983 and 1997 Nāpau Crater area eruptions. These magmas were stored in physically and chemically distinct pods at depths of 1·6–3·0 km prior to mixing with new magma intruded from the summit to produce the episode 59 lava. One pod contained a fractionated equivalent of 1983 lava, and the other a hybrid of compositions similar to 1983 and 1997 lavas. The petrology of episode 59 lava demonstrates that magmas from two previous eruptions (1983 and 1997) were available to mix with magma intruded from the summit region. This study clarifies the pre-eruptive history of the mixed episode 59 lava, and elucidates the evolution of the volcano's magmatic system in a region of frequent eruptions.

Hawaii↗
USGS2012

Genetic analysis of a novel nidovirus from fathead minnows

A bacilliform virus was isolated from diseased fathead minnows ( Pimephales promelas ). Analysis of the complete genome coding for the polyprotein (pp1ab), spike (S), membrane (M) and nucleocapsid (N) proteins revealed that the virus was most like white bream virus (WBV), another bacilliform virus isolated from white bream ( Blicca bjoerkna L.) and the type species of the genus Bafinivirus within the order Nidovirales . In addition to similar gene order and size, alignment of deduced amino acid sequences of the pp1ab, M, N and S proteins of the fathead minnow nidovirus (FHMNV) with those of WBV showed 46, 44, 39 and 15 % identities, respectively. Phylogenetic analysis using the conserved helicase domain of the replicase showed FHMNV was distinct from WBV, yet the closest relative identified to date. Thus, FHMNV appears to represent a second species in the genus Bafinivirus . A PCR assay was developed for the identification of future FHMNV-like isolates.

Journal of General Virology↗
USGS2004

Isolation and characterization of a rhabdovirus from starry flounder (Platichthys stellatus) collected from the northern portion of Puget Sound, Washington, USA

The initial characterization of a rhabdovirus isolated from a single, asymptomatic starry flounder (Platichthys stellatus) collected during a viral survey of marine fishes from the northern portion of Puget Sound, Washington, USA, is reported. Virions were bullet-shaped and approximately 100 nm long and 50 nm wide, contained a lipid envelope, remained stable for at least 14 days at temperatures ranging from -80 to 5 degrees C and grew optimally at 15 degrees C in cultures of epithelioma papulosum cyprini (EPC) cells. The cytopathic effect on EPC cell monolayers was characterized by raised foci containing rounded masses of cells. Pyknotic and dark-staining nuclei that also showed signs of karyorrhexis were observed following haematoxylin and eosin, May-Grunwald Giemsa and acridine orange staining. PAGE of the structural proteins and PCR assays using primers specific for other known fish rhabdoviruses, including Infectious hematopoietic necrosis virus, Viral hemorrhagic septicemia virus, Spring viremia of carp virus, and Hirame rhabdovirus, indicated that the new virus, tentatively termed starry flounder rhabdovirus (SFRV), was previously undescribed in marine fishes from this region. In addition, sequence analysis of 2678 nt of the amino portion of the viral polymerase gene indicated that SFRV was genetically distinct from other members of the family Rhabdoviridae for which sequence data are available. Detection of this virus during a limited viral survey of wild fishes emphasizes the void of knowledge regarding the diversity of viruses that naturally infect marine fish species in the North Pacific Ocean.

Journal of General Virology↗