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At least 253 records · Page 14Linked to original sources

Survey for blood parasites in redheads (Aythya americana) wintering at the Chandeleur Islands, Louisiana

We detected no infections with species of Plasmodium , Haemoproteus , Leucocytozoon , Sarcocystis or Trypanosoma in blood smears, liver and spleen impressions, and muscle tissue from 136 redheads ( Aythya americana ) collected or captured at the Chandeleur Islands, Louisiana (USA), during three winters (1987 to 1990). One bird, a juvenile male, was infected with an unidentified species of microfilaria. Thus, we found no evidence that hematozoa had an effect on redheads during the course of the study.

Louisiana

Lead poisoning of spectacled eiders (Somateria fischeri) and of a common eider (Somateria mollissima) in Alaska

Lead poisoning was diagnosed in four spectacled eiders ( Somateria fischeri ) and one common eider ( Somateria mollissima ) found dead or moribund at the Yukon Delta National Wildlife Refuge, Alaska (USA) in 1992, 1993, and 1994. Ingested lead shot was found in the lower esophagus of one spectacled eider and in the gizzard of the common eider. Lead concentrations in the livers of the spectacled eiders were 26 to 38 ppm wet weight, and 52 ppm wet weight in the liver of the common eider. A blood sample collected from one of the spectacled eiders before it was euthanized had a lead concentration of 8.5 ppm wet weight. This is the first known report of lead poisoning in the spectacled eider, recently listed as a threatened species by the U.S. Fish and Wildlife Service.

Alaska

An alternative bacteriological medium for the isolation of Aeromonas spp.

Two solid bacteriologic media were compared for cultivating Aeromonas spp. from piscine sources: the Rimler-Shotts (RS) medium and a starch-glutamate-ampicillin-penicillin-based medium (SGAP-10C) used for the recovery of Aeromonas spp. from water samples. The selective and differential capacities of the media were assessed March through October 1992 by recovery rate and phenotype of 99 isolates representing 15 genera of bacteria. Recovery frequency of Aeromonas spp. ( n = 62) was similar at 97% on RS and 95% on SGAP-10C. The SGAP-10C medium proved to be more specific than RS toward Aeromonas species (P ≤ 0.005). Use of SGAP-10C at 24 C for 48 hr offers a better choice for the laboratory recovery of Aeromonas spp. from clinical fish specimens.

Journal of Wildlife Diseases

Bothrigaster variolaris (Trematoda: Cyclocoelidae) infection in two Florida snail kites (Rostrhamus sociabilis plumbeus)

Two Florida snail kites ( Rostrhamus sociabilis plumbeus , Say) collected in August and December 1993 from Osceola County (28°15′N, 81°17′W) and Glades County (26°45′N, 81°10′W), Florida (USA), respectively, were infected with Bothrigaster variolaris (Trematoda: Cyclocoelidae); this parasite has not been reported previously from birds in the United States. One bird, a fledgling female, harbored 270 specimens of B. variolaris ; the helminths caused airsacculitis and bronchitis, possible contributing factors in the death of this bird. The other bird, a subadult female, was infected with 40 trematodes and had gross and microscopic lesions consistent with trauma as the cause of death.

Florida

Mortality of passerines adjacent to a North Carolina corn field treated with granular carbofuran

Red-winged blackbirds ( Agelaius phoeniceus ) were collected during an epizootic in southeastern North Carolina (USA). Activity of brain cholinesterase (ChE) was inhibited by 14 to 48% in three of five specimens, and returned to normal levels after incubation. Gastrointestinal tracts were analyzed for 30 anti-ChE agents. Carbofuran, the only compound detected, was present in all specimens at levels from 5.44 to 72.7 μg/g wet weight. Application of granular carbofuran in an adjacent corn field, results of necropsy examinations, and chemical analyses are consistent with a diagnosis of carbofuran poisoning in these specimens.

North Carolina

Exposure of wild waterfowl to Mycoplasma anatis

We developed an ELISA procedure to assess the presence of M. Anatis-specific serum antibody in ducks. Sera from exposed and unexposed Pekin ducks (Anas platyrhynchos) were used to standardize tile ELISA and to establish reference ranges to classify ELISA results as exposed or not exposed. We conducted serological surveys of female waterfowl in the central and eastern United States between 1988 and 1992 to assess the frequency of exposure in wild waterfowl. Adult breeding mallards (Anas platyrhynchos), wintering mallards, and black ducks (Anas rubripes) had high prevalences of exposure to M. Anatis (25% to >80%). In comparison, none of the breeding adult canvasbacks (Aythya valisineria) had serum antibody levels indicating exposure. Approximately 50% of the juvenile mallards and black ducks were exposed to M. Anatis by 8 months of age, indicating high transmission rates among wild birds.

Journal of Wildlife Diseases

Diagnostic criteria for selenium toxicosis in aquatic birds: dietary exposure, tissue concentrations, and macroscopic effects

A feeding study with mallard ducks ( Anas platyrhynchos ) was conducted during March to July 1988 in Laurel, Maryland (USA), to identify diagnostic criteria for selenium toxicosis in birds. One-year-old male mallards in groups of 21 were fed diets containing 0, 10, 20, 40, or 80 parts per million (ppm) selenium, as seleno-DL-methionine, for 16 weeks. All ducks receiving 80 ppm died. Ducks receiving 40 or 80 ppm selenium consumed less feed than ducks in the other treatment groups. Body weights of ducks receiving 40 or 80 ppm selenium declined during the study. The post-breeding molt was delayed in ducks receiving 40 ppm; most ducks receiving 80 ppm selenium died prior to the onset of molt. At necropsy, numerous abnormalities were observed in ducks that died but only a small number of abnormalities were observed in ducks surviving to the end of the study in the 40 ppm group. Weights of the heart, spleen, and pancreas were mostly lower and weights of the kidney were higher for ducks dying during the study than for euthanized ducks. Liver weights were unaffected. Selenium accumulated in soft tissues approximately in proportion to dietary concentrations. Selenium concentrations in tissues of all ducks that died were different from those of surviving ducks in the 0, 10, and 20 ppm groups, but were not different from those of surviving ducks in the 40 ppm group. Proposed diagnostic criteria for fatal chronic selenosis were derived from body weight, macroscopic abnormalities, organ weights, and concentrations of selenium in the liver. Proposed diagnostic criteria for non-fatal chronic selenosis were derived from body weight, plumage condition, macroscopic abnormalities, concentrations of selenium in the liver, reproductive failure, and alterations of blood and tissue chemistries. Lead or dioxin poisoning have diagnostic criteria most similar to selenium toxicosis.

Journal of Wildlife Diseases

Weights, hematology and serum chemistry of seven species of free-ranging tropical pelagic seabirds

I established reference values for weight, hematology, and serum chemistry for seven species of free-ranging Hawaiian tropical pelagic seabirds comprising three orders (Procellariiformes, Pelecaniformes, Charadriiformes) and six families (Procellariidae, Phaethontidae, Diomedeidae, Sulidae, Fregatidae, and Laridae). Species examined included 84 Hawaiian dark-rumped petrels ( Pterodoma phaeopygia ), 90 wedge-tailed shearwaters ( Puffinus pacificus ), 151 Laysan albatrosses ( Diomedea immutabilis ), 69 red-footed boobies ( Sula sula ), 154 red-tailed tropicbirds ( Phaeton rubricauda ), 90 great frigatebirds ( Fregata minor ), and 72 sooty terns ( Sterna fuscata ). Hematocrit, total plasma solids, total and differential white cell counts, serum glucose, calcium, phosphorus, uric acid, total protein, albumin, globulin, aspartate aminotransferase and creatinine phosphokinase were analyzed. Among and within species, hematology and chemistry values varied with age, sex, season, and island of collection. Despite this variation, order-wide trends were observed.

Hawai'i

Toxicity of white phosphorus to waterfowl: Acute exposure in mallards

As part of an effort to understand extensive, white phosphorus (P4)-induced waterfowl mortality at Eagle River Flats, Fort Richardson, Alaska, we conducted a number of acute toxicity tests using penned mallards (Anas platyrhynchos) in 1993 and 1994. The 24-hr median lethal dose (LD50) for P4 dissolved in oil was 6.46 mg/kg in adult males and 6.96 mg/kg in adult females. Although the median lethal doses were not statistically different, the female dose-response curve had a statistically shallower slope than that of males. The LD50 for the ecologically more relevant pelletized form of P4 in adult males was 4.05 mg/kg. In mallards, one mechanism of P4 toxicity caused rapid (3 to 10 hr) mortality and had signs consistent with anoxia. A second, slower acting mechanism resulted in hepatic and renal pathology including extensive fat deposition in the liver and cellular necrosis. White phosphorus accumulated in adipose tissues, but only for a few days.

Journal of Wildlife Diseases

Efficacy of eastern encephalitis immunization in whooping cranes

An epizootic of eastern equine encephalitis (EEE) at the Patuxent Wildlife Research Center (PWRC), Laurel, Maryland (USA), in 1989 provided an opportunity to determine if EEE immunization protected whooping cranes ( Grus americana ). Based on seroconversion of 31% of sympatric hatch-year sandhill cranes, Grus canadensis , and a previous 35% case fatality rate in whooping cranes, 17 (37%) of the 46 susceptible whooping cranes should have been exposed to virus and six should have died. As there were no deaths in these birds, the EEE vaccination program appeared to be efficacious in this whooping crane population.

Journal of Wildlife Diseases

Pasteurella multocida serotype 1 isolated from a lesser snow goose

Pharyngeal swabs were collected from 298 lesser snow geese (Chen caerulescens caerulescens) at Banks Island (Northwest Territories. Canada) in the summer of 1994. Pasteurella multocida serotype 1 was isolated from an adult male bird and P. multocida serotype 3 was isolated from an adult female goose. Pathogenicity of the serotype 1 isolate was confirmed by inoculation in Pekin ducks (Anas platyrhynchos). The serotype 3 isolate was non-pathogenic in Pekin ducks. This is the first documented isolation of pathogenic P. multocida serotype 1 from apparently healthy wild snow geese.

Northwest Territories

Diagnostic criteria for selenium toxicosis in aquatic birds: Histologic lesions

Chronic selenium toxicosis was induced in 1-yr-old male mallard ducks ( Anas platyrhynchos ) by feeding selenium, as seleno-DL-methionine, in amounts of 0, 10, 20, 40, and 80 parts per million (ppm) to five groups of 21 ducks each for 16 wk during March to July 1988. All mallards in the 80 ppm group, three in the 40 ppm group, and one in the 20 ppm group died. Histologic lesions in mallards that died of selenosis were hepatocellular vacuolar degeneration progressing to centrolobular and panlobular necrosis, nephrosis, apoptosis of pancreatic exocrine cells, hypermaturity and avascularity of contour feathers of the head with atrophy of feather follicles, lymphocytic necrosis and atrophy of lymphoid organs (spleen, gut-associated lymphoid tissue, and lumbar lymph nodes), and severe atrophy and degeneration of fat. Histologic lesions in surviving mallards in the 40 ppm group, which had tissue residues of selenium comparable to mallards that died, were fewer and much milder than mallards that died; lesions consisted of atrophy of lymphoid tissue, hyalinogranular swelling of hepatoeytes, atrophy of seminiferous tubules, and senescence of feathers. No significant histologic lesions were detected in euthanized mallards in the 0, 10 and 20 ppm groups. Based on tissue residues and histologic findings, primarily in the liver, there was a threshold of selenium accumulation above which pathophysiologic changes were rapid and fatal. Pathognomonic histologic lesions of fatal and nonfatal selenosis were not detected. Criteria for diagnosis of fatal selenosis in aquatic birds include consistent histologic lesions in the liver, kidneys, and organs of the immune system. Although histologic changes were present in cases of chronic non-fatal selenosis, these were inconsistent. Consistent features of fatal and non-fatal chronic selenosis were marked weight loss and elevated concentrations of selenium in organs.

Journal of Wildlife Diseases

Hematologic and serum biochemical reference intervals for Florida panthers

Ninety-four blood samples were collected from 48 (29 males and 19 females) free-ranging Florida panthers ( Felis concolor coryi ) captured in southern Florida (USA) from 1983 to 1994 for routine hematological and serum biochemical analysis. Florida panthers in the northern portion of their range had significantly higher red blood cell (mean ± SD = 7.923 × 10 6 ± 0.854 × 10 6 /μl), hemoglobin (12.53 ± 1.66 g/dl), and packed cell volume (36.97 ± 4.27%) values compared to those of panthers localized in more southern parts of Florida (7.148 × 10 6 ± 1.045 × 10 6 /μl, 11.60 ± 1.62 g/dl, and 34.82 ± 5.99%, respectively). Adults had significantly higher mean serum total protein (7.50 ± 0.59 g/dl) and packed cell volume (36.90 ± 4.97%) values than juveniles (6.88 ± 0.49 g/dl and 34.54 ± 5.30%). However, mean serum albumin concentrations were significantly higher in juveniles (3.80 ± 0.26 g/dl) when compared to adult values (3.58 ± 0.26 g/dl). Mean serum calcium concentrations were significantly higher in juveniles (10.33 ± 0.39 mg/dl) than in adults (9.66 ± 0.45 mg/dl). Additionally, mean serum iron concentrations were significantly higher in those panthers of intergrade genetic stock compared to values in those of authentic genetic stock (105.6 ± 72.1 μg/dl versus 59.3 ± 19.7 μg/dl, respectively).

Florida

Epidermal tumors of rainbow smelt with associated virus

Epithelial tumors of the skin occurred in landlocked populations of rainbow smelt ( Osmerus mordax ) in several lakes in New Hampshire (USA) during the spawning runs. Histologically, these were noninvasive epithelial cell lesions. Herpesvirus-like particles could be seen in the nucleus and cytoplasm. The lesions occurred in both males and females. Prevalence, which varied annually, was as high as 30%.

Journal of Wildlife Diseases

Sensitivity of condition indices to changing density in a white-tailed deer population

The ways in which comprehensive condition profiles, incorporating morphometric, histologic, physiologic, and diet quality indices, responded to changes in density of a white-tailed deer ( Odocoileus virginianus ) population were examined. Changes in these condition indices were monitored in a northeastern Oklahoma deer herd as density declined from peaks of 80 and 72 deer/km 2 in 1989 and 1990 (high-density) to lows of 39 and 41 deer/km 2 in 1991 and 1992 (reduced-density), respectively. Compared to a reference population (6 deer/km 2 ), deer sampled during high-density exhibited classic signs of nutritional stress such as low body and visceral organ masses (except elevated adrenal gland mass), low fecal nitrogen levels, reduced concentrations of serum albumin, elevated serum creatinine concentrations, and a high prevalence of parasitic infections. Although density declined by one half over the 4-yr study, gross indices of condition (in particular body mass and size) remained largely unchanged. However, selected organ masses, serum albumin and non-protein nitrogen constituents, and fecal nitrogen indices reflected improvements in nutritional status with reductions in density. Many commonly used indices of deer condition (fat reserves, hematocrit, total serum protein, and blood urea nitrogen) were not responsive to fluctuations in density.

Journal of Wildlife Diseases

Evaluation of two oral baiting systems for wild rodents

Tetracycline hydrochloride (TC)-treated peanut butter or rodent chow baits were distributed during March 1990, on separate 0.53 ha sites in Oglethorpe County, Georgia (USA). Rodents were trapped on a control site prior to bait distribution and on two baited sites 6 days post-distribution. Cleaned skulls from euthanized mammals were grossly examined for TC florescence using an ultraviolet (UV) light. Mandibles were sectioned and examined for TC fluorescence using an ultraviolet light microscope. All 21 cotton rats ( Sigmodon hispidus ), four eastern harvest mice ( Rithrodontomys humulis ), and two golden mice ( Ochrotomys nuttalli ) captured on the control site were negative for TC fluorescence. On the peanut butter bait site, mandible sections from 29 of 32 (91%) cotton rats, three of three (100%) eastern harvest mice, two of three (66%) golden mice, zero of five (0%) white-footed mice ( Peromyscus leucopus ), one of three (33%) short-tailed shrews ( Blarina brevicauda ), and zero of two (0%) least shrews ( Cryptotis parva ) were positive for TC. Results from the rodent chow bait site indicated that 18 of 25 (72%) cotton rats, zero of three (0%) eastern harvest mice, two of seven (29%) golden mice, zero of four (0%) white-footed mice, and zero of four (0%) least shrews were positive for TC fluorescence in mandible sections. These results suggest that a large portion of a free-ranging small rodent population can be administered biological markers or vaccines using baits.

Georgia