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At least 253 records · Page 14Linked to original sources
USGS2020

Comparison of SELDM simulated total-phosphorus concentrations with ecological impervious-area criteria

Ecological studies indicate that impervious cover (IC) greater than approximately 5%–20% may have adverse effects on receiving-stream ecology. It is difficult to separate the effects of runoff quality from other effects of urbanization on receiving streams. This study presents the results of a numerical experiment to assess the effects of increasing IC on water quality using the Stochastic Empirical Loading and Dilution Model (SELDM). Hydrologic and physiographic variables representative of southern New England were used to simulate receiving water quality in a basin with IC ranging from 0.1% to 30%. Simulation results mirror the results of ecological studies; event mean concentrations (EMCs) of total phosphorus (TP) increase proportionally to the logarithms of imperviousness for a given risk percentile. Simulation results indicated that commonly used stormwater treatment methods may be insufficient for mitigating the effects of imperviousness. Therefore, disconnection, rather than treatment, may be needed to protect water quality, and efforts to preserve undeveloped stream basins may be more effective than efforts to remediate conditions in highly developed basins. Results also indicate that commonly used water-quality criteria may be too restrictive for stormwater because TP EMCs frequently exceed these criteria, even in minimally developed basins.

Journal of Environmental Engineering↗
USGS2025

Review: The hydrogeology of critical mineral resources relevant to the energy transition

Attaining the goals of the international treaty on climate change (the Paris Agreement) will greatly increase the demand for the critical minerals required to implement clean-energy technologies. This poses both challenges and opportunities to the hydrogeologic community from several perspectives. Here, important insights that the hydrogeological sciences have to offer for mineral exploration, mineral production, and addressing environmental issues related to mining and mine decommissioning are summarized. This study focuses on copper, cobalt, lithium, and rare earths to represent the broad spectrum of critical minerals and illustrate their relevance by referring to projected demands and production rates. The current understanding of the hydrogeologic processes that form major deposits of these minerals are then summarized. Ore is defined as the naturally occurring material from which minerals of economic value can be extracted, where most ore deposits are the products of complex hydrogeologic couplings between fluid flow, heat transport, solute transport, chemical reactions, and mechanical deformation. Exploration models for the discovery of deeper, hidden deposits are potentially informed by hydrogeologic theory and hydrogeochemical processes. Hydrogeologic understanding and methods are also essential to production and recovery. Longstanding challenges are mine dewatering and (conversely) mine water supply, as well as mineral-extraction practices such as spoil heap leaching and in situ mining. New challenges arise from element extraction from subsurface brines. Finally, the quantity of water use and potential environmental impacts of mining on water quality are at the core of ‘social license’: the approval and acceptance of society to mining activities.

Hydrogeology Journal↗
USGS2025

Physical habitat is more than a sediment issue: A multi-dimensional habitat assessment indicates new approaches for river management

Degraded physical habitat is a common stressor affecting river ecosystems and typically addressed in the United States (US) through a regulatory focus on sediment. However, a narrow regulatory focus on sediment may overlook other aspects of physical habitat and the processes for its creation, maintenance, and degradation. In addition, there exist few “ready-to-use” regional assessments of the multiple dimensions of physical habitat to better understand continuous patterns of condition and prioritize management efforts across a large spatial scale. In this study, we use rapid habitat monitoring data to train a machine-learning ( i.e., random forest) model to predict twelve physical habitat metrics for nearly 120,000 km of nontidal rivers and streams across the Chesapeake Bay watershed, US. We capture a range of habitat conditions driven by both natural variables and anthropogenic pressures. Covariation among habitat metrics indicated two major dimensions of habitat variation: 1) coarse bed substrate and hydromorphic heterogeneity and 2) bank stability and riparian condition. The model predicted localized changes from 2001 to 2019, and the predicted areas of deterioration roughly balanced improvements across the watershed, indicating little progress towards long-term watershed management goals. To evaluate connections to regulatory and management endpoints, we compared our physical habitat predictions to paired estimates of sediment and flow alteration across the region. Sediment concentrations were greater in reaches with less bank stability and lower riparian quality; however, the relation was weak for coarse bed condition metrics, including embeddedness, which is frequently used for establishing regulatory sediment restrictions. For flow alteration, most habitat metrics had lower scores with altered flow metrics, but metrics of instream habitat heterogeneity and coarse substrate condition were most strongly affected. Increased flashy, high flows negatively affected most metrics, but coarse substrate metrics were also negatively affected by greater low flow severity. This study highlights a potential disconnect between a narrow focus on regulatory sediment targets given the multiple dimensions and responses of physical habitat. A more holistic approach to physical habitat in management interventions – one that considers hydromorphic processes, diversity and variability in microhabitats, and explicit consideration of alterations to both low and high flows – may be warranted. By providing direct estimates of multiple aspects of physical habitat, this model can help support managers in the Chesapeake Bay watershed to better understand the range of habitat conditions, identify high-quality reaches for conservation, and target potential management actions tailored to localized conditions.

Delaware, Maryland, New York, Pennsylvania, Virgin↗
USGS2012

Effects of the amphibian chytrid fungus and four insecticides on Pacific treefrogs ( Pseudacris regilla )

Chemical contamination may influence host-pathogen interactions, which has implications for amphibian population declines. We examined the effects of four insecticides alone or as a mixture on development and metamorphosis of Pacific Treefrogs ( Pseudacris regilla ) in the presence or absence of the amphibian chytrid fungus ( Batrachochytrium dendrobatidis [Bd]). Bd exposure had a negative impact on tadpole activity, survival to metamorphosis, time to metamorphosis, and time of tail absorption (with a marginally negative effect on mass at metamorphosis); however, no individuals tested positive for Bd at metamorphosis. The presence of sublethal concentrations of insecticides alone or in a mixture did not impact Pacific Treefrog activity as tadpoles, survival to metamorphosis, or time and size to metamorphosis. Insecticide exposure did not influence the effect of Bd exposure. Our study did not support our prediction that effects of Bd would be greater in the presence of expected environmental concentrations of insecticide(s), but it did show that Bd had negative effects on responses at metamorphosis that could reduce the quality of juveniles recruited into the population.

Journal of Herpetology↗
USGS2005

The World Coal Quality Inventory: A status report

National and international policy makers and industry require accurate information on coal, including coal quality data, to make informed decisions regarding international import needs and export opportunities, foreign policy, technology transfer policies, foreign investment prospects, environmental and health assessments, and byproduct use and disposal issues. Unfortunately, the information needed is generally proprietary and does not exist in the public domain. The U.S. Geological Survey (USGS), in conjunction with partners in about 60 countries, is developing a digital compilation of worldwide coal quality. The World Coal Quality Inventory (WoCQI) will contain coal quality information for samples obtained from major coal beds in countries having significant coal production, as well as from many countries producing smaller volumes of coal, with an emphasis on coals currently being burned. The information that will be incorporated includes, but is not limited to, proximate and ultimate analyses; sulfur-form data; major, minor, and trace element analysis; and semi-quantitative analyses of minerals, modes of occurrence, and petrography. The coal quality information will eventually be linked to a Geographic Information System (GIS) that shows the coal basins and sample locations along with geologic, land use, transportation, industrial, and cultural information. The WoCQI will be accessible on the USGS web page and new data added periodically. This multi-national collaboration is developing global coal quality data that contain a broad array of technologic, economic, and environmental parameters, which should help to ensure the efficient and environmentally compatible use of global coal resources in the 21st century.

International Journal of Coal Geology↗
USGS2020

Nontuberculous mycobacterial disease and molybdenum in Colorado watersheds

Nontuberculous mycobacteria (NTM) are environmental bacteria that may cause chronic lung disease. Environmental factors that favor NTM growth likely increase the risk of NTM exposure within specific environments. We aimed to identify water-quality constituents (Al, As, Cd, Ca, Cu, Fe, Pb, Mg, Mn, Mo, Ni, K, Se, Na, Zn, and pH) associated with NTM disease across Colorado watersheds. We conducted a geospatial, ecological study, associating data from patients with NTM disease treated at National Jewish Health and water-quality data from the Water Quality Portal. Water-quality constituents associated with disease risk were identified using generalized linear models with Poisson-distributed discrete responses. We observed a highly robust association between molybdenum (Mo) in the source water and disease risk. For every 1- unit increase in the log concentration of molybdenum in the source water, disease risk increased by 17.0%. We also observed a statistically significant association between calcium (Ca) in the source water and disease risk. The risk of NTM varied by watershed and was associated with watershed-specific water-quality constituents. These findings may inform mitigation strategies to decrease the overall risk of exposure.

Colorado↗
USGS1999

The types of data needed for assessing the environmental and human health impacts of coal

Coal is one of the most important sources of energy. Its worldwide use will continue to expand during the next several decades, particularly in rapidly developing countries such as China and India. Unfortunately, coal use may bring with it environmental and human health costs. Many of the environmental and health problems attributed to coal combustion are due to mobilization of potentially toxic elements. Some of these problems could be minimized or even avoided if comprehensive databases containing appropriate coal quality information were available to decision makers so that informed decisions could be made regarding coal use. Among the coal quality parameters that should be included in these databases are: C, H, N, O, pyritic sulfur, organic sulfur, major, minor, and trace element concentrations, modes of occurrence of environmentally sensitive elements, cleanability, mineralogy, organic chemistry, petrography, and leachability.Coal is one of the most important sources of energy. Its worldwide use will continue to expand during the next several decades, particularly in rapidly developing countries such as China and India. Unfortunately, coal use may bring with it environmental and human health costs. Many of the environmental and health problems attributed to coal combustion are due to mobilization of potentially toxic elements. Some of these problems could be minimized or even avoided if comprehensive databases containing appropriate coal quality information were available to decision makers so that informed decisions could be made regarding coal use. Among the coal quality parameters that should be included in these databases are: C, H, N, O, pyritic sulfur, organic sulfur, major, minor, and trace element concentrations, modes of occurrence of environmentally sensitive elements, cleanability, mineralogy, organic chemistry, petrography, and leachability.

International Journal of Coal Geology↗
USGS1984

Avian models for toxicity testing

The use of birds as test models in experimental and environmental toxicology as related to health effects is reviewed, and an overview of descriptive tests routinely used in wildlife toxicology is provided. Toxicologic research on birds may be applicable to human health both directly by their use as models for mechanistic and descriptive studies and indirectly as monitors of environmental quality. Topics include the use of birds as models for study of teratogenesis and embryotoxicity, neurotoxicity, behavior, trends of environmental pollution, and for use in predictive wildlife toxicology. Uses of domestic and wild-captured birds are discussed.

Journal of the American College of Toxicology↗
USGS2023

Support for management actions to protect night sky quality: Insights from visitors to state and national park units in the U.S.

Light pollution is a global phenomenon where anthropogenic light sources continue to grow unabated, affecting both social and ecological systems. This is leaving parks and protected areas as some of the last vestiges of naturally dark environments for protecting views of the night sky. Yet, even parks and protected areas have outdoor lighting. Alternative lighting practices are needed to reduce or prevent light pollution from within parks. However, making parks darker may not be desirable for some visitors if they believe it will reduce navigability, safety, or restrict how they recreate (e.g., requiring the use of red-light flashlights after dark and before dawn). How visitors will respond to alternative lighting practices that park managers can implement is still unknown. We used an on-site intercept survey at nine state and national park units in Utah, U.S. , to investigate nighttime visitors' support or opposition to management actions to protect night sky quality and their interest in learning about topics related to night skies. Further, this study also segmented visitors into two groups: those ‘dependent’ on the dark sky as a resource and those whose activities did not depend on a dark sky. Defining what a ‘dark sky dependent’ visitor is, which has yet to be done in the literature, is a fundamental step to furthering night sky research and management efforts. Across nine parks and protected areas, 62% of nighttime visitors participated in dark sky dependent activities. Findings indicate broad support for management actions designed to improve night sky quality, with between 74% and 89% of all visitors supporting seven different management actions. There was stronger support from dark sky dependent visitors for some elements of alternative lighting practices, but there was still strong support for those who do not participate in dark sky dependent outdoor recreation. Additionally, between 57% and 75% of visitors were interested in learning more about topics related to night skies. This research indicates most visitors would welcome actions to preserve the quality of the rapidly dwindling naturally dark experiences offered by parks and protected areas.

Utah↗
USGS2002

Groundwater contamination downstream of a contaminant penetration site. II. Horizontal penetration of the contaminant plume

Part I of this study (Rubin, H.; Buddemeier, R.W. Groundwater Contamination Downstream of a Contaminant Penetration Site Part 1: Extension-Expansion of the Contaminant Plume. J. of Environmental Science and Health Part A (in press).) addressed cases, in which a comparatively thin contaminated region represented by boundary layers (BLs) developed within the freshwater aquifer close to contaminant penetration site. However, at some distance downstream from the penetration site, the top of the contaminant plume reaches the top or bottom of the aquifer. This is the location of the "attachment point," which comprises the entrance cross section of the domain evaluated by the present part of the study. It is shown that downstream from the entrance cross section, a set of two BLs develop in the aquifer, termed inner and outer BLs. It is assumed that the evaluated domain, in which the contaminant distribution gradually becomes uniform, can be divided into two sections, designated: (a) the restructuring section, and (b) the establishment section. In the restructuring section, the vertical concentration gradient leads to expansion of the inner BL at the expense of the outer BL, and there is almost no transfer of contaminant mass between the two layers. In the establishment section, each of the BLs occupies half of the aquifer thickness, and the vertical concentration gradient leads to transfer of contaminant mass from the inner to the outer BL. By use of BL approximations, changes of salinity distribution in the aquifer are calculated and evaluated. The establishment section ends at the uniformity point, downstream from which the contaminant concentration profile is practically uniform. The length of the restructuring section, as well as that of the establishment section, is approximately proportional to the aquifer thickness squared, and is inversely proportional to the transverse dispersivity. The study provides a convenient set of definitions and terminology that are helpful in visualizing the gradual development of uniform contaminant concentration distribution in an aquifer subject to contaminant plume penetration. The method developed in this study can be applied to a variety of problems associated with groundwater quality, such as initial evaluation of field data, design of field data collection, the identification of appropriate boundary conditions for numerical models, selection of appropriate numerical modeling approaches, interpretation and evaluation of field monitoring results, etc.

Journal of Environmental Science and Health - Part↗
USGS2021

Informed breeding dispersal following stochastic changes to patch quality in a pond-breeding amphibian

The unidirectional movement of animals between breeding patches (i.e. breeding dispersal) has profound implications for the ecological and evolutionary dynamics of spatially structured populations. In spatiotemporally variable environments, individuals are expected to adjust their dispersal decisions according to information gathered on the environmental and/or social cues that reflect the fitness prospects in a given breeding patch (i.e. informed dispersal). A paucity of empirical work limited our understanding of the ability of animals to depart from low-quality breeding patches and settle in high-quality breeding patches. We examined the capacity of individuals to respond to stochastic changes in habitat quality via informed breeding dispersal in a pond-breeding amphibian. We conducted a 5-year (2015–2019) capture–recapture study of boreal toads Anaxyrus boreas boreas ( n = 1,100) that breed in beaver ponds in western Wyoming, USA. During early spring of 2017, an extreme flooding event destroyed several beaver dams and resulted in the loss of breeding habitat. We used multi-state models to investigate how temporal changes in pond characteristics influenced breeding dispersal, and determine whether movement decisions were in accordance with prospects for reproductive fitness. Boreal toads more often departed from low-quality breeding ponds (without successful metamorphosis) and settled in high-quality breeding ponds (with successful metamorphosis). Movement decisions were context-dependent and associated with pond characteristics altered by beaver dam destruction. Individuals were more likely to depart from shallow ponds with high vegetation cover and settle in deep ponds with low vegetation cover. The probability of metamorphosis was related to the same environmental cues, suggesting that boreal toads assess the fitness prospects of a breeding patch and adjust movement decisions accordingly (i.e. informed breeding dispersal). We demonstrated that stochastic variability in environmental conditions and habitat quality can underpin dispersal behaviour in amphibians. Our study highlighted the mechanistic linkages between habitat change, movement behaviour and prospects for reproductive performance, which is critical for understanding how wild animals respond to rapid environmental change.

Wyoming↗
USGS2015

U.S. recreational water quality criteria: a vision for the future

This manuscript evaluates the U.S. Recreational Water Quality Criteria (RWQC) of 2012, based upon discussions during a conference held 11–13 March 2013, in Honolulu, Hawaii. The RWQC of 2012 did not meet expectations among the research community because key recommended studies were not completed, new data to assess risks to bathers exposed to non-point sources of fecal indicator bacteria (FIB) were not developed, and the 2012 RWQC did not show marked improvements in strategies for assessing health risks for bathers using all types of recreational waters. The development of the 2012 RWQC was limited in scope because the epidemiologic studies at beach sites were restricted to beaches with point sources of pollution and water samples were monitored for only enterococci. The vision for the future is development of effective RWQC guidelines based on epidemiologic and quantitative microbial risk assessment (QMRA) studies for sewage specific markers, as well as human enteric pathogens so that health risks for bathers at all recreational waters can be determined. The 2012 RWQC introduced a program for states and tribes to develop site-specific water quality criteria, and in theory this approach can be used to address the limitations associated with the measurements of the traditional FIB.

International Journal of Environmental Research an↗
USGS1996

Trail degradation as influenced by environmental factors: A state-of-the-knowledge review

Excerpt: Human use and misuse of land has been causing extensive degradation of the very natural resources on which we depend. National parks, wilderness and other protected natural or semi-natural areas (referred to as natural areas hereafter) represent efforts to preserve our natural heritage from further exploitation. Such areas also provide outstanding recreational, research, and educational opportunities. However, resource impacts resulting from overuse and inappropriate management increasingly threaten these protected areas and erode their natural and cultural values. Among the many forms of recreational impact, those associated with trail development and use are often a major concern of natural area managers and visitors. Such impacts impair and degrade the functions that trails serve, including (1) protecting resources by concentrating traffic on a hardened tread, (2) providing recreational opportunities along aesthetically pleasing trail routes, and (3) facilitating recreational use by providing a transportation network. The extensive distribution of trails and their degrading condition in many natural areas can have pervasive environmental effects through alteration of natural drainage patterns, erosion and deposition of soil, introduction of exotic vegetation, and increasing human-wildlife conflicts. Degraded trails also threaten the quality of visitor experiences by making travel difficult or unsafe, or by diminishing visitors ?

Journal of Soil and Water Conservation↗
USGS1996

Recovery of burrowing mayflies (Ephemeroptera: Ephemeridae: Hexagenia ) in western Lake Erie

Burrowing mayflies ( Hexagenia spp.) are native to western Lake Erie and were abundant until the 1950s, when they disappeared due to degraded water and sediment quality. Nymphs were absent from the sediments of most of western Lake Erie after the 1950s, although small, widely disjunct populations apparently persisted near shore. Sediment samples collected in 1993 revealed several small populations near the western and southern shores and beyond the mouths of the Detroit and Maumee rivers. A larger population was found in the southern island area, but nymphs were absent in the middle of the basin. By 1995, nymphs had spread throughout the western half and eastern end of the basin but remained absent from the middle of the basin. These data indicate that Hexagenia began recolonizing nearshore areas before offshore areas. Increasingly large swarms of winged Hexagenia on shore and over the lake between 1992 and 1994 further indicate that mayflies are recolonizing the basin. Factors that have permitted Hexagenia recovery in western Lake Erie probably include improved sediment and water quality attributed to pollution abatement programs implemented after the early 1970s, and perhaps environmental changes in the early 1990s attributed to effects of the exotic zebra mussel ( Dreissena polymorpha ).

Journal of Great Lakes Research↗
USGS2007

Assessments of habitat preferences and quality depend on spatial scale and metrics of fitness

1. Identifying the habitat features that influence habitat selection and enhance fitness is critical for effective management. Ecological theory predicts that habitat choices should be adaptive, such that fitness is enhanced in preferred habitats. However, studies often report mismatches between habitat preferences and fitness consequences across a wide variety of taxa based on a single spatial scale and/or a single fitness component. 2. We examined whether habitat preferences of a declining shrub steppe songbird, the Brewer's sparrow Spizella breweri, were adaptive when multiple reproductive fitness components and spatial scales (landscape, territory and nest patch) were considered. 3. We found that birds settled earlier and in higher densities, together suggesting preference, in landscapes with greater shrub cover and height. Yet nest success was not higher in these landscapes; nest success was primarily determined by nest predation rates. Thus landscape preferences did not match nest predation risk. Instead, nestling mass and the number of nesting attempts per pair increased in preferred landscapes, raising the possibility that landscapes were chosen on the basis of food availability rather than safe nest sites. 4. At smaller spatial scales (territory and nest patch), birds preferred different habitat features (i.e. density of potential nest shrubs) that reduced nest predation risk and allowed greater season-long reproductive success. 5. Synthesis and applications. Habitat preferences reflect the integration of multiple environmental factors across multiple spatial scales, and individuals may have more than one option for optimizing fitness via habitat selection strategies. Assessments of habitat quality for management prescriptions should ideally include analysis of diverse fitness consequences across multiple ecologically relevant spatial scales. ?? 2007 The Authors.

Journal of Applied Ecology↗
USGS2018

Monitoring responses to variation in food supply for a migratory waterfowl: American Black Duck (Anas rubripes) in winter

Wintering Black Ducks ( Anas rubripes ) concentrate in wetlands along the Atlantic coast where natural and anthropogenic disturbances have increased over the last 50 years, a period in which the population of Black Ducks has declined. We studied the sensitivity of Black Ducks to perturbations in food supply that often result from disturbances by storms, predators, and people. In the paper, we characterize the responses of captive Black Ducks to shifts in food quality and availability during winter and apply those measures to a comparison of wild birds. Captive ducks that were fed intermittently (3 consecutive days/week) compensated for fasted days to achieve similar body mass and body fat to control birds that were fed every day on both animal- and plant-based diets. However, birds that were fed intermittently expended 15% more energy each day than controls when both groups were fed (536 vs. 464 kJ/kg 0.75 ), which indicates that variable food supply increases the costs of maintenance and thus reduces the number of birds that can be supported on the same resource of food without interruptions to foraging. Egg production was not affected by diet quality provided in spring or by the frequency of feeding during the preceding winter months. Black Ducks lost body fat through winter in captivity and in the wild. Fat stores of birds in New Jersey were greater than those of birds in Maine (13.3 vs. 8.3% of body mass) in January, which reflected the high energy demands of cold temperatures in Maine. Values for ∂ 15 N were greater in Maine than in New Jersey for both red blood cells and plasma, which indicated a consistent diet of marine invertebrates in Maine. Greater isotopic variation in red blood cells indicated that diets were more diverse in New Jersey than in Maine for both ∂ 15 N (9.7 ± 1.1 vs. 11.2 ± 0.4‰) and for ∂ 13 C (− 15.1 ± 2.2 vs. − 13.8 ± 1.4‰). Plasma ∂ 13 C was enriched over red blood cells in wild birds especially those with low fat stores, which suggested birds with low energy stores were shifting diets. Black Ducks can compensate for disturbances in feeding by increasing intakes if they have access to high quality wetlands where they are able to find abundant food. High energy demands at cold temperatures may constrain fat stores and thus the tolerance of feeding disturbances especially at the northern limits of the winter range. We hypothesize that decreasing variation in diet may indicate an increase in vulnerability to disturbance in winter when body fat is low. Recent efforts to assess and improve habitat quality of Black Ducks could be enhanced by monitoring the body composition and diet of birds to assess their vulnerability to disturbances in food supply and energy demands.

Journal of Comparative Physiology B: Biochemical, ↗
USGS2018

Current research in land, water, and agroecosystems: ASABE journals 2017 year in review

This article highlights current research into land and water resources, agroecosystems, and agricultural production systems published by the Natural Resources and Environmental Systems (NRES) community of ASABE journals (Transactions of the ASABE and Applied Engineering in Agriculture) in 2017. This article reviews the context, scope, and key results of the published articles and perhaps more importantly recommends areas for increased research attention. Experimental and modeling advances were described in hydrology, agroecosystems, climate-change effects, soil erosion, irrigation, drainage, forest resources, livestock systems, natural treatment systems, international water issues, and water quality topic areas. Three special collections were published (International Watershed Technology, Crop Modeling to Optimize Water Use, and Advances in Drainage). Other focal areas included 14 articles relating to livestock waste management, 13 concerning irrigated agricultural systems, 8 addressing climate change effects on land and water resources, and 16 on various aspects of soil erosion measurement and modeling. Building on the articles reviewed from 2017 and toward a vision of future agroecosystems research, the NRES community of ASABE journals strives to grow its role in making new knowledge accessible to sustain agricultural and natural systems in a changing world. In this vane, recommendations for future research direction are discussed with an emphasis on increased application of remote sensing data to agroecosystems research, improved assessment of agroecosystem resiliency and vulnerability to land and climate change, development of integrated models of agroecosystem services, meeting stubborn water management challenges in agricultural production systems, and focusing on publishing fully reproducible model results.

Transactions of the ASABE↗
USGS2004

Detection of human enteric viruses in stream water with RT-PCR and cell culture

A multiplex RT-PCR method was used to measure virus occurrence at five stream water sites that span a range of hydroclimatic, water-quality, and land-use characteristics. The performance of the molecular method was evaluated in comparison with traditional cell culture and Escherichia coli membrane filtration assays. The study incorporated multiple quality controls and included a control for virus recovery during the sampling procedure as well as controls to detect potentially false-negative and false-positive data. Poliovirus recovery ranged from 16 to 65% and was variable, even in samples collected within the same stream. All five sites were positive for viruses by both molecular and cell culture-based virus assays. Enteroviruses, reoviruses, rotaviruses, and hepatitis A viruses were detected, but the use of the quality controls proved critical for interpretation of the molecular data. All sites showed evidence of faecal contamination, and culturable viruses were detected in four samples that would have met the US Environmental Protection Agency's recommended E. coli guideline for safe recreational water.

Journal of Water and Health↗