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At least 253 records · Page 14Linked to original sources

Prioritizing chemicals of ecological concern in Great Lakes tributaries using high-throughput screening data and adverse outcome pathways

Chemical monitoring data were collected in surface waters from 57 Great Lakes tributaries from 2010-13 to identify chemicals of potential biological relevance and sites at which these chemicals occur. Traditional water-quality benchmarks for aquatic life based on in vivo toxicity data were available for 34 of 67 evaluated chemicals. To expand evaluation of potential biological effects, measured chemical concentrations were compared to chemical-specific biological activities determined in high-throughput (ToxCast) in vitro assays. Resulting exposure-activity ratios (EARs) were used to prioritize the chemicals of greatest potential concern: 4-nonylphenol, bisphenol A, metolachlor, atrazine, DEET, caffeine, tris(2-butoxyethyl) phosphate, tributyl phosphate, triphenyl phosphate, benzo(a)pyrene, fluoranthene, and benzophenone. Water-quality benchmarks were unavailable for five of these chemicals, but for the remaining seven, EAR-based prioritization was consistent with that based on toxicity quotients calculated from benchmarks. Water-quality benchmarks identified three additional PAHs (anthracene, phenanthrene, and pyrene) not prioritized using EARs. Through this analysis, an EAR of 10-3 was identified as a reasonable threshold above which a chemical might be of potential concern. To better understand apical hazards potentially associated with biological activities captured in ToxCast assays, in vitro bioactivity data were matched with available adverse outcome pathway (AOP) information. The 49 ToxCast assays prioritized via EAR analysis aligned with 23 potentially-relevant AOPs present in the AOP-Wiki. Mixture effects at monitored sites were estimated by summation of EAR values for multiple chemicals by individual assay or individual AOP. Commonly predicted adverse outcomes included impacts on reproduction and mitochondrial function. The EAR approach provided a screening-level assessment for evidence-based prioritization of chemicals and sites with potential for adverse biological effects. The approach aids prioritization of future monitoring activities and provides testable hypotheses to help focus those efforts. This also expands the fraction of detected chemicals for which biologically-based benchmark concentrations are available to help contextualize chemical monitoring results.

Science of the Total Environment

Bacterial ethane formation from reduced, ethylated sulfur compounds in anoxic sediments

Trace levels of ethane were produced biologically in anoxic sediment slurries from five chemically different aquatic environments. Gases from these locations displayed biogenic characteristics, having 12 C-enriched values of δ 13 CH 4 (−62 to −86%.), δ 13 C 2 H 6 (−35 to −55%.) and high ratios (720 to 140,000) of CH 4 [C 2 H 6 + C 3 H 8 ] "> CH4[C2H6+ C3H8] . Endogenous production of ethane by slurries was inhibited by autoclaving or by addition of the inhibitor of methanogenic bacteria, 2-bromoethanesulfonic acid (BES). Ethane formation was stimulated markedly by ethanethiol (ESH), and, to a lesser extent, by diethylsulfide (DES). Formation of methane and ethane in ESH- or DES-amended slurries was blocked by BES. Experiments showed that ethionine (or an analogous compound) could be a precursor of ESH. Ethylamine or ethanol additions to slurries caused only a minor stimulation of ethane formation. Similarly, propanethiol additions resulted in only a minor enhancement of propane formation. Cell suspensions of a methyltrophic methanogen produced traces of ethane when incubated in the presence of DES, although the organism did not grow on this compound. These results indicate that methanogenic bacteria produce ethane from the traces of ethylated sulfur compounds present in recent sediments. Preliminary estimates of stable carbon isotope fractionation associated with sediment methane formation from dimethylsulfide was about 40%., while ethane formation from DES and ESH was only 4. 6 and 6.5%., respectively.

Geochimica et Cosmochimica Acta

Limestone fluidized bed treatment of acid-impacted water at the Craig Brook National Fish Hatchery, Maine, USA

Decades of atmospheric acid deposition have resulted in widespread lake and river acidification in the northeastern U.S. Biological effects of acidification include increased mortality of sensitive aquatic species such as the endangered Atlantic salmon (Salmo salar). The purpose of this paper is to describe the development of a limestone-based fluidized bed system for the treatment of acid-impacted waters. The treatment system was tested at the Craig Brook National Fish Hatchery in East Orland, Maine over a period of 3 years. The product water from the treatment system was diluted with hatchery water to prepare water supplies with three different levels of alkalinity for testing of fish health and survival. Based on positive results from a prototype system used in the first year of the study, a larger demonstration system was used in the second and third years with the objective of decreasing operating costs. Carbon dioxide was used to accelerate limestone dissolution, and was the major factor in system performance, as evidenced by the model result: Alk = 72.84 ?? P(CO2)1/2; R2 = 0.975. No significant acidic incursions were noted for the control water over the course of the study. Had these incursions occurred, survivability in the untreated water would likely have been much more severely impacted. Treated water consistently provided elevated alkalinity and pH above that of the hatchery source water. ?? 2005 Elsevier B.V. All rights reserved.

Aquacultural Engineering

Moving environmental DNA methods from concept to practice for monitoring aquatic macroorganisms

The discovery that macroorganisms can be detected from their environmental DNA (eDNA) in aquatic systems has immense potential for the conservation of biological diversity. This special issue contains 11 papers that review and advance the field of eDNA detection of vertebrates and other macroorganisms, including studies of eDNA production, transport, and degradation; sample collection and processing to maximize detection rates; and applications of eDNA for conservation using citizen scientists. This body of work is an important contribution to the ongoing efforts to take eDNA detection of macroorganisms from technical breakthrough to established, reliable method that can be used in survey, monitoring, and research applications worldwide. While the rapid advances in this field are remarkable, important challenges remain, including consensus on best practices for collection and analysis, understanding of eDNA diffusion and transport, and avoidance of inhibition in sample collection and processing. Nonetheless, as demonstrated in this special issue, eDNA techniques for research and monitoring are beginning to realize their potential for contributing to the conservation of biodiversity globally.

Biological Conservation

Microbial reduction of uranium

Reduction of the soluble, oxidized form of uranium, U(VI), to insoluble U(IV) is an important mechanism for the immobilization of uranium in aquatic sediments and for the formation of some uranium ores 1–10 . U(VI) reduction has generally been regarded as an abiological reaction in which sulphide, molecular hydrogen or organic compounds function as the reductant 1,2,5,11 . Microbial involvement in U(VI) reduction has been considered to be limited to indirect effects, such as microbial metabolism providing the reduced compounds for abiological U(VI) reduction and microbial cell walls providing a surface to stimulate abiological U(VI) reduction 1,12,13 . We report here, however, that dissimilatory Fe(III)-reducing microorganisms can obtain energy for growth by electron transport to U(VI). This novel form of microbial metabolism can be much faster than commonly cited abiological mechanisms for U(VI) reduction. Not only do these findings expand the known potential terminal electron acceptors for microbial energy transduction, they offer a likely explanation for the deposition of uranium in aquatic sediments and aquifers, and suggest a method for biological remediation of environments contaminated with uranium.

Nature

Comparing efficiency of American Fisheries Society standard snorkeling techniques to environmental DNA sampling techniques

Analysis of environmental DNA (eDNA) is an emerging technique used to detect aquatic species through water sampling and the extraction of biological material for amplification. Our study compared the efficacy of eDNA methodology to American Fisheries Society (AFS) standard snorkeling surveys with regard to detecting the presence of rare fish species. Knowing which method is more efficient at detecting target species will help managers to determine the best way to sample when both traditional sampling methods and eDNA sampling are available. Our study site included three Navajo Nation streams that contained Navajo Nation Genetic Subunit Bluehead Suckers Catostomus discobolus and Zuni Bluehead Suckers C. discobolus yarrowi . We first divided the entire wetted area of streams into consecutive 100-m reaches and then systematically selected 10 reaches/stream for snorkel and eDNA surveys. Surface water samples were taken in 10-m sections within each 100-m reach, while fish presence was noted via snorkeling in each 10-m section. Quantitative PCR was run on each individual water sample in quadruplicate to test for the presence or absence of the target species. With eDNA sampling techniques, we were able to positively detect both species in two out of the three streams. Snorkeling resulted in positive detection of both species in all three streams. In streams where the target species were detected with eDNA sampling, snorkeling detected fish at 11–29 sites/stream, whereas eDNA detected fish at 3–12 sites/stream. Our results suggest that AFS standard snorkeling is more effective than eDNA for detecting target fish species. To improve our eDNA procedures, the amount of water collected and tested should be increased. Additionally, filtering water on-site may improve eDNA techniques for detecting fish. Future research should focus on standardization of eDNA sampling to provide a widely operational sampling tool.

Arizona, New Mexico

A riverscape perspective of Pacific salmonids and aquatic habitats prior to large-scale dam removal in the Elwha River, Washington, USA

Dam removal has been increasingly proposed as a river restoration technique. In 2011, two large hydroelectric dams will be removed from Washington State’s Elwha River. Ten anadromous fish populations are expected to recolonise historical habitats after dam removal. A key to understanding watershed recolonisation is the collection of spatially continuous information on fish and aquatic habitats. A riverscape approach with an emphasis on biological data has rarely been applied in mid-sized, wilderness rivers, particularly in consecutive years prior to dam removal. Concurrent snorkel and habitat surveys were conducted from the headwaters to the mouth (rkm 65–0) of the Elwha River in 2007 and 2008. This riverscape approach characterised the spatial extent, assemblage structure and patterns of relative density of Pacific salmonids. The presence of dams influenced the longitudinal patterns of fish assemblages, and species richness was the highest downstream of the dams, where anadromous salmonids still have access. The percent composition of salmonids was similar in both years for rainbow trout, Oncorhynchus mykiss (Walbaum), coastal cutthroat trout, Oncorhynchus clarkii clarkii (Richardson) (89%; 88%), Chinook salmon, Oncorhynchus tshawytscha (Walbaum) (8%; 9%), and bull trout, Salvelinus confluentus (Suckley) (3% in both years). Spatial patterns of abundance for rainbow and cutthroat trout ( r = 0.76) and bull trout ( r = 0.70) were also consistent between years. Multivariate and univariate methods detected differences in habitat structure along the river profile caused by natural and anthropogenic factors. The riverscape view highlighted species-specific biological hotspots and revealed that 60–69% of federally threatened bull trout occurred near or below the dams. Spatially continuous surveys will be vital in evaluating the effectiveness of upcoming dam removal projects at restoring anadromous salmonids.

Washington

Diet of non-native northern snakehead ( Channa argus ) compared to three co-occurring predators in the lower Potomac River, USA

Introductions of large, non-native, carnivorous fishes continue to occur worldwide and represent a substantial management concern to global biodiversity. One of the most recent non-native fishes to successfully establish in North America is the northern snakehead ( Channa argus ), found in the lower Potomac River catchment. Dispersal of the northern snakehead throughout this system has been well documented since its original discovery in May 2004; however, little is known about the foraging habits of this species and its interactions with co-occurring predators. Here, we quantify northern snakehead diet in comparison with the diets of naturalised largemouth bass ( Micropterus salmoides ), and native American eel ( Anguilla rostrata ) and yellow perch ( Perca flavescens ) collected from tidal freshwaters bordering Virginia and Maryland near Fort Belvoir, Virginia. Over 97% of northern snakehead gut contents were fishes, with fundulid and centrarchid species consumed most frequently. Dietary overlap was biologically significant only between northern snakehead and largemouth bass. Aquatic invertebrates were >10 times more common in native predator diets, reducing dietary overlap with northern snakehead. Ontogenic shifts in adult northern snakehead diet were also detected, which may be explained by optimal foraging rather than true prey specificity. Northern snakehead may be occupying a novel niche based on a piscivorous diet, therefore limiting competition with resident predators in the lower Potomac River. Further research into interactions between largemouth bass and northern snakehead is needed to inform management decisions and understand the ecological impacts of this non-native species.

Maryland, Virginia

Effects of seasonal hypoxia on macroinvertebrate communities in a small reservoir

Localized hypoxia can reduce available habitat, restrict movement and limit the abundance of aquatic invertebrates. Cultural eutrophication coupled with the effects of climate change is likely to increase the frequency and extent of hypoxia in aquatic ecosystems, yet little is known about how oxygen gradients in small reservoirs influence spatial distribution and abundance of aquatic invertebrates. We evaluated the effects of environmental and biological attributes on seasonal and spatial variation of macroinvertebrates and explored how hypoxic conditions influenced littoral, benthic and pelagic macroinvertebrate communities in Lake Alvin, South Dakota. We collected data on reservoir conditions in conjunction with macroinvertebrate sampling from May to October, 2009-2011 and applied an information theoretic approach to evaluate factors affecting invertebrate abundance. Hypoxic conditions were present from May to September in the lacustrine zone impacting 10─39% of the water column. Benthic invertebrates were typically absent from the lacustrine zone during periods of severe hypoxia and were most abundant in the shallow, well-oxygenated riverine zone. Littoral invertebrates were negatively related to percent of the water column that was hypoxic suggesting that fish, confined to shallow water by hypoxia, may be consuming a larger portion of littoral invertebrates in their diets. Cladocera and Copepoda densities were influenced primarily by water depth and monthly precipitation. The larger size of Daphnia found in the hypoxic-prone transitional and lacustrine zones suggest that low oxygen concentration may provide a refuge from fish predation. This study demonstrated that spatial variation in near-bottom oxygen concentrations were important predictors of macroinvertebrate and zooplankton abundance and size structure in Lake Alvin and that macroinvertebrates, particularly benthic and littoral invertebrates, could benefit from measures taken to reduce summer hypoxia.

South Dakota

Nitrogen and phosphorus balance of grass carp, Ctenopharyngodon idella, fed elodea, Egeria denea

The herbivorous grass carp fed winter elodea exhibited a negative nitrogen balance in metabolism, suggesting that food having a higher protein content would be required for normal growth. About a third of the phosphorus contained in food was retained; hence, a combination of aquatic plants and grass carp might afford an effective biological method for removing phosphorus from water. Water quality was altered by the excretion of ammonium and orthophosphate by grass carp. Even though the grass carp might be useful in removing some phosphorus from water, its principal impact was to increase the rate of recycling of nitrogen and phosphorus.

Transactions of the American Fisheries Society

Benthic algae of benchmark streams in agricultural areas of eastern Wisconsin

Benthic algae were collected from 20 streams in the Western Lake Michigan Drainages by the U.S. Geological Survey in May and June of 1993 as part of the National Water-Quality Assessment program. These streams were selected to represent "benchmark" streams that were minimally affected by human activities, especially agriculture, for comparison to other streams in similar environmental settings. Streams were chosen from four relatively homogeneous units (RHU's) in agricultural areas with differing texture of surficial deposits and bedrock type. Blue-green algae were the dominant algal cells at all but 5 of the 20 stream sites, and the most abundant species at these sites was Calothrix parietina, a nitrogen-fixer typically found in pristine streams. Most of the taxa at all sites were diatoms. The dominant diatom guilds observed were the Achnanthes spp., erect forms, and Navicula spp. Except for three streams thought to have low productivity, the Shannon-Wiener diversity index for diatoms was high at all benchmark streams and indicated either minor stress or no stress on the diatom community. With regard to water quality, additional diatom indexes for 17 of 20 benchmark streams indicated no pollution effects and no significant siltation. All benchmark streams had good to excellent biological integrity and either minor or no impairment of aquatic life with regard to diatoms. A variety of algal metrics and relative abundances of diatom morphological guilds correlated with basin-, segment-and reach-level habitat characteristics, including drainage area, basin drainage density, basin soil permeability, Q/Q2 (instantaneous discharge measured at time of sampling divided by the estimated 2-year flood discharge), stream length, and average width of natural riparian vegetation. Algal taxa richness decreased with higher percentages of agricultural land and lower percentages of forested land. The relative abundance of pollution-tolerant diatoms was higher in streams where the basin land was primarily agricultural as compared to forested. The Shannon- Wiener diversity index for diatoms, the percentage of diatom taxa, and the percent relative abundances of diatom cells, pollution tolerant diatoms, Achnanthes spp., erect diatom forms, nitrogen-fixing algae, and blue-green algae differed significantly among either RHU's or ecoregions. Higher abundances of pollution-sensitive diatoms and a higher pollution index indicate that water quality in sampled streams in the North Central Hardwood Forests ecoregion may be less degraded than in streams in the Southeastern Wisconsin Till Plains ecoregion. Algal taxa richness decreased as specific conductance, dissolved nitrate plus nitrite, and suspended sediment increased. This relation may indicate a negative effect of agricultural activities on the algal taxa richness of the stream. Pollution-tolerant diatoms and the pollution index increased as these and additional factors correlated with agriculture increased. Multivariate analyses indicated multiple scales of environmental factors affect algae. Although two-way indicator species analysis (TWINSPAN), detrended correspondence analysis (DCA), and canonical correspondence analysis (CCA) generally separated sites according to RHU, only DCA ordination indicated a separation of sites according to ecoregion. Environmental variables con-elated with DCA axes 1 and 2 and therefore indicated as important explanatory factors for algal distribution and abundance were factors related to stream size, basin land use/cover, geomorphology, hydrogeology, and riparian disturbance. CCA analyses with a more limited set of environmental variables indicated that pH, average width of natural riparian vegetation (segment scale), basin land use/cover and Q/Q2 were the most important variables affecting the distribution and relative abundance of benthic algae at the 20 benchmark streams,

Wisconsin

Distribution, migration behavior, habitat use, and species interactions of fall-released juvenile hatchery spring Chinook salmon on the Deschutes River, Oregon, 2002, Annual report 2002

In a review of National Fish Hatcheries (NFH), the U.S. Fish and Wildlife Service (USFWS) identified the need to assess the fate of hatchery-reared fish and their potential effect on the aquatic community (USFWS 1998). Additionally, in the Columbia River Biological Opinion, the National Marine Fisheries Service (NMFS) recommended monitoring and evaluating ecological interactions between hatchery and wild fish (NMFS 1999). In 2002, a study was designed to investigate the fate of hatchery-reared fish and to assess habitat use and fish interactions in the Deschutes River, Oregon.

Oregon

Applications of a broad-spectrum tool for conservation and fisheries analysis: Aquatic gap analysis

Natural resources support all of our social and economic activities, as well as our biological existence. Humans have little control over most of the physical, biological, and sociological conditions dictating the status and capacity of natural resources in any particular area. However, the most rapid and threatening influences on natural resources typically are anthropogenic overuse and degradation. In addition, living natural resources (i.e., organisms) do not respect political boundaries, but are aware of their optimal habitat and environmental conditions. Most organisms have wider spatial ranges than the jurisdictional boundaries of environmental agencies that deal with them; even within those jurisdictions, information is patchy and disconnected. Planning and projecting effects of ecological management are difficult, because many organisms, habitat conditions, and interactions are involved. Conservation and responsible resource use involves wise management and manipulation of the aspects of the environment and biological communities that can be effectively changed. Tools and data sets that provide new insights and analysis capabilities can enhance the ability of resource managers to make wise decisions and plan effective, long-term management strategies. Aquatic gap analysis has been developed to provide those benefits. Gap analysis is more than just the assessment of the match or mis-match (i.e., gaps) between habitats of ecological value and areas with an appropriate level of environmental protection (e.g., refuges, parks, preserves), as the name suggests. Rather, a Gap Analysis project is a process which leads to an organized database of georeferenced information and previously available tools to examine conservation and other ecological issues; it provides a geographic analysis platform that serves as a foundation for aquatic ecological studies. This analytical tool box allows one to conduct assessments of all habitat elements within an area of interest. Aquatic gap analysis naturally focuses on aquatic habitats. The analytical tools are largely based on specification of the species-habitat relations for the system and organism group of interest (Morrison et al. 2003; McKenna et al. 2006; Steen et al. 2006; Sowa et al. 2007). The Great Lakes Regional Aquatic Gap Analysis (GLGap) project focuses primarily on lotic habitat of the U.S. Great Lakes drainage basin and associated states and has been developed to address fish and fisheries issues. These tools are unique because they allow us to address problems at a range of scales from the region to the stream segment and include the ability to predict species specific occurrence or abundance for most of the fish species in the study area. The results and types of questions that can be addressed provide better global understanding of the ecological context within which specific natural resources fit (e.g., neighboring environments and resources, and large and small scale processes). The geographic analysis platform consists of broad and flexible geospatial tools (and associated data) with many potential applications. The objectives of this article are to provide a brief overview of GLGap methods and analysis tools, and demonstrate conservation and planning applications of those data and tools. Although there are many potential applications, we will highlight just three: (1) support for the Eastern Brook Trout Joint Venture (EBTJV), (2) Aquatic Life classification in Wisconsin, and (3) an educational tool that makes use of Google Earth (use of trade or product names does not imply endorsement by the U.S. Government) and Internet accessibility.

Gap Analysis Bulletin

Changes in biological communities of the Fountain Creek Basin, Colorado, 2003–2016, in relation to antecedent streamflow, water quality, and habitat

The analysis described in this report is part of a longterm project monitoring the biological communities, habitat, and water quality of the Fountain Creek Basin. Biology, habitat, and water-quality data have been collected at 10 sites since 2003. These data include annual samples of aquatic invertebrate communities, fish communities, water quality, and quantitative riverine habitat. This report examines trends in biological communities from 2003 to 2016 and explores relationships between biological communities and abiotic variables (antecedent streamflow, physical habitat, and water quality). Six biological metrics (three invertebrate and three fish) and four individual fish species were used to examine trends in these data and how streamflow, habitat, and (or) water quality may explain these trends. The analysis of 79 trends shows that the majority of significant trends decreased over the trend period. Overall, 19 trends before adjustments for streamflow in the fish (12) and invertebrate (7) metrics were all decreasing except for the metric Invertebrate Species Richness at the most upstream site in Monument Creek. Seven of these trends were explained by streamflow and four trends were revealed that were originally masked by variability in antecedent streamflow. Only two sites (Jimmy Camp Creek at Fountain, CO and Fountain Creek near Pinon, CO) had no trends in the fish or invertebrate metrics. Ten of the streamflow-adjusted trends were explained by habitat, one was explained by water quality, and five were not explained by any of the variables that were tested. Overall, from 2003 to 2016, all the fish metric trends were decreasing with an average decline of 40 percent, and invertebrate metrics decreased on average by 9.5 percent. A potential peak streamflow threshold was identified above which there is severely limited production of age-0 flathead chub (Platygobio gracilis).

Colorado

Threats and stressors to the health of the ecosystems of Lakes Mead and Mohave: Chapter 6 in A synthesis of aquatic science for management of Lakes Mead and Mohave

Ecosystem impacts from visitor activities or natural environmental change are important concerns in all units of the National Park system. Possible impacts to aquatic ecosystems at Lake Mead National Recreation Area (LMNRA) are of particular concern because of the designation of Lakes Mead and Mohave as critical habitat for the federally listed endangered razorback sucker (Xyrauchen texanus), the significance of the sport fishery, and the regional importance of its habitats to more than 90 documented species of waterbirds. Potential threats to shoreline habitats are of concern not only for their ecosystem values but also for maintaining the recreational setting. Many areas adjacent to the shorelines of Lakes Mead and Mohave are designated wilderness areas. For purposes of this document, stressors are any chemical, biological, or physical agent that has a detrimental effect on aquatic ecosystems at the organism, population, or community level. Human-made stressors at Lakes Mead and Mohave include direct effects of recreation on the lakes, like boating and fishing, as well as indirect effects of activities away from the lakes, such as growing population and increasing urbanization. Common natural environmental stressors include extended changes in climate (precipitation or temperature), or the erosion, transport, and loading of chemical constituents in rocks and sediments to aquatic environments. Human activity also can exacerbate natural stressors in a variety of ways.

Circular

Occurrence of endocrine active compounds and biological responses in the Mississippi River— Study design and data, June through August 2006

Concern that selected chemicals in the environment may act as endocrine active compounds in aquatic ecosystems is widespread; however, few studies have examined the occurrence of endocrine active compounds and identified biological markers of endocrine disruption such as intersex occurrence in fish longitudinally in a river system. This report presents environmental data collected and analyzed by the U.S. Geological Survey, Minnesota Pollution Control Agency and St. Cloud State University as part of an integrated biological and chemical study of endocrine disruption in fish in the Mississippi River. Data were collected from water, bed sediment, and fish at 43 sites along the river from the headwaters at Lake Itasca to 14 miles downstream from Brownsville, Minnesota during June through August 2006. Twenty-four individual compounds were detected in water samples, with cholesterol, atrazine, N,N-diethyl-meta-toluamide, metolachlor, and hexahydrohexamethylcyclopentabenzopyran detected most frequently (in at least 10 percent of the samples). The number of compounds detected in water per site ranged from 0 to 8. Forty individual compounds were detected in bed-sediment samples. The most commonly detected compounds (in at least 50 percent of the samples) were indole, beta-sitosterol, cholesterol, beta-stigmastanol, 3-methyl-1H-indole, p-cresol, pyrene, phenol, fluoranthene, 3-beta coprostanol, benzo[a]pyrene, acetophenone, and 2,6-dimethylnaphthalene. The total number of detections in bed sediment (at a site) ranged from 3 to 31. The compounds NP1EO, NP2EO, and 4-nonylphenol were detected in greater than 10 percent of the samples. Most (80 percent) female fish collected had measurable concentrations of vitellogenin. Vitellogenin also was detected in 62, 63, and 33 percent of male carp, smallmouth bass, and redhorse, respectively. The one male walleye sample plasma sample analyzed had a vitellogenin detection. Vitellogenin concentrations were lower in male fish (not detected to 10.80 micrograms per milliliter) than female fish (0.04 to 248,079 micrograms per milliliter). Gonadosomatic Index values ranged from 0.02 to 7.49 percent among all male fish and were greater for male carp than for the other three species. No intersex (oocytes present in testes tissue) was found in any male fish sampled.

Minnesota, Wisconsin

Featured collection introduction: Connectivity of streams and wetlands to downstream waters

Connectivity is a fundamental but highly dynamic property of watersheds. Variability in the types and degrees of aquatic ecosystem connectivity presents challenges for researchers and managers seeking to accurately quantify its effects on critical hydrologic, biogeochemical, and biological processes. However, protecting natural gradients of connectivity is key to protecting the range of ecosystem services that aquatic ecosystems provide. In this featured collection, we review the available evidence on connections and functions by which streams and wetlands affect the integrity of downstream waters such as large rivers, lakes, reservoirs, and estuaries. The reviews in this collection focus on the types of waters whose protections under the U.S. Clean Water Act have been called into question by U.S. Supreme Court cases. We synthesize 40+ years of research on longitudinal, lateral, and vertical fluxes of energy, material, and biota between aquatic ecosystems included within the Act's frame of reference. Many questions about the roles of streams and wetlands in sustaining downstream water integrity can be answered from currently available literature, and emerging research is rapidly closing data gaps with exciting new insights into aquatic connectivity and function at local, watershed, and regional scales. Synthesis of foundational and emerging research is needed to support science‐based efforts to provide safe, reliable sources of fresh water for present and future generations.

Journal of the American Water Resources Associatio

Eastern Ecological Science Center — Fish and aquatic animal health

Science provided by the U.S. Geological Survey (USGS) Eastern Ecological Science Center (EESC) helps to protect fish and aquatic animal health and support disease management of our Nation’s fisheries and aquatic animal populations. Our capabilities and expertise span diverse, yet highly integrated disciplines related to all aspects of biology, ecology, and animal health. Our scientists work to identify novel and emerging pathogens and diseases to assess fish and aquatic animal responses to parasites, pathogens, adverse environmental conditions, and chemical exposures. Eastern Ecological Science Center research is conducted both in laboratories and in the field in various aquatic environments ranging from headwater streams to the ocean. The EESC team provides science leadership and support for fisheries and aquatic species specialists nationwide.

Fact Sheet