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USGS2014

Reproductive health indicators of fishes from Pennsylvania watersheds: association with chemicals of emerging concern

Fishes were collected at 16 sites within the three major river drainages (Delaware, Susquehanna, and Ohio) of Pennsylvania. Three species were evaluated for biomarkers of estrogenic/antiandrogenic exposure, including plasma vitellogenin and testicular oocytes in male fishes. Smallmouth bass Micropterus dolomieu , white sucker Catostomus commersonii , and redhorse sucker Moxostoma species were collected in the summer, a period of low flow and low reproductive activity. Smallmouth bass were the only species in which testicular oocytes were observed; however, measurable concentrations of plasma vitellogenin were found in male bass and white sucker. The percentage of male bass with testicular oocytes ranged from 10 to 100 %, with the highest prevalence and severity in bass collected in the Susquehanna drainage. The percentage of males with plasma vitellogenin ranged from 0 to 100 % in both bass and sucker. Biological findings were compared with chemical analyses of discrete water samples collected at the time of fish collections. Estrone concentrations correlated with testicular oocytes prevalence and severity and with the percentage of male bass with vitellogenin. No correlations were noted with the percentage of male sucker with vitellogenin and water chemical concentrations. The prevalence and severity of testicular oocytes in bass also correlated with the percent of agricultural land use in the watershed above a site. Two sites within the Susquehanna drainage and one in the Delaware were immediately downstream of wastewater treatment plants to compare results with upstream fish. The percentage of male bass with testicular oocytes was not consistently higher downstream; however, severity did tend to increase downstream.

Pennsylvania↗

USGS2014

Correspondence of biological condition models of California streams at statewide and regional scales

We used boosted regression trees (BRT) to model stream biological condition as measured by benthic macroinvertebrate taxonomic completeness, the ratio of observed to expected (O/E) taxa. Models were developed with and without exclusion of rare taxa at a site. BRT models are robust, requiring few assumptions compared with traditional modeling techniques such as multiple linear regression. The BRT models were constructed to provide baseline support to stressor delineation by identifying natural physiographic and human land use gradients affecting stream biological condition statewide and for eight ecological regions within the state, as part of the development of numerical biological objectives for California’s wadeable streams. Regions were defined on the basis of ecological, hydrologic, and jurisdictional factors and roughly corresponded with ecoregions. Physiographic and land use variables were derived from geographic information system coverages. The model for the entire state ( n  = 1,386) identified a composite measure of anthropogenic disturbance (the sum of urban, agricultural, and unmanaged roadside vegetation land cover) within the local watershed as the most important variable, explaining 56 % of the variance in O/E values. Models for individual regions explained between 51 and 84 % of the variance in O/E values. Measures of human disturbance were important in the three coastal regions. In the South Coast and Coastal Chaparral, local watershed measures of urbanization were the most important variables related to biological condition, while in the North Coast the composite measure of human disturbance at the watershed scale was most important. In the two mountain regions, natural gradients were most important, including slope, precipitation, and temperature. The remaining three regions had relatively small sample sizes ( n  ≤ 75 sites) and had models that gave mixed results. Understanding the spatial scale at which land use and land cover affect taxonomic completeness is imperative for sound management. Our results suggest that invertebrate taxonomic completeness is affected by human disturbance at the statewide and regional levels, with some differences among regions in the importance of natural gradients and types of human disturbance. The construction and application of models similar to the ones presented here could be useful in the planning and prioritization of actions for protection and conservation of biodiversity in California streams.

California↗

USGS2015

Presence and abundance of non-native plant species associated with recent energy development in the Williston Basin

The Williston Basin, located in the Northern Great Plains, is experiencing rapid energy development with North Dakota and Montana being the epicenter of current and projected development in the USA. The average single-bore well pad is 5 acres with an estimated 58,485 wells in North Dakota alone. This landscape-level disturbance may provide a pathway for the establishment of non-native plants. To evaluate potential influences of energy development on the presence and abundance of non-native species, vegetation surveys were conducted at 30 oil well sites (14 ten-year-old and 16 five-year-old wells) and 14 control sites in native prairie environments across the Williston Basin. Non-native species richness and cover were recorded in four quadrats, located at equal distances, along four transects for a total of 16 quadrats per site. Non-natives were recorded at all 44 sites and ranged from 5 to 13 species, 7 to 15 species, and 2 to 8 species at the 10-year, 5-year, and control sites, respectively. Respective non-native cover ranged from 1 to 69, 16 to 76, and 2 to 82 %. Total, forb, and graminoid non-native species richness and non-native forb cover were significantly greater at oil well sites compared to control sites. At oil well sites, non-native species richness and forb cover were significantly greater adjacent to the well pads and decreased with distance to values similar to control sites. Finally, non-native species whose presence and/or abundance were significantly greater at oil well sites relative to control sites were identified to aid management efforts.

Montana, North Dakota↗

USGS2015

An evaluation of a bed instability index as an indicator of habitat quality in mountain streams of the northwestern United States

Managers of aquatic resources benefit from indices of habitat quality that are reproducible and easy to measure, demonstrate a link between habitat quality and biota health, and differ between human-impacted (i.e., managed) and reference (i.e., nonimpacted or minimally impacted) conditions. The instability index (ISI) is an easily measured index that describes the instability of a streambed by relating the tractive force of a stream at bankfull discharge to the median substrate size. Previous studies have linked ISI to biological condition but have been limited to comparisons of sites within a single stream or among a small number of streams. We tested ISI as an indicator of human impact to habitat and biota in mountain streams of the northwestern USA. Among 1428 sites in six northwestern states, ISI was correlated with other habitat measures (e.g., residual pool depth, percent fine sediment) and indices of biotic health (e.g., number of intolerant macroinvertebrate taxa, fine sediment biotic index) and differed between managed and reference sites across a range of stream types and ecoregions. While ISI could be useful in mountain streams throughout the world, this index may be of particular interest to aquatic resource managers in the northwestern USA where a large dataset, from which ISI can be calculated, exists.

Montana, Idaho, Utah, Nevada, Oregon, Washington↗

USGS2016

Storm-event-transport of urban-use pesticides to streams likely impairs invertebrate assemblages

Insecticide use in urban areas results in the detection of these compounds in streams following stormwater runoff at concentrations likely to cause toxicity for stream invertebrates. In this 2013 study, stormwater runoff and streambed sediments were analyzed for 91 pesticides dissolved in water and 118 pesticides on sediment. Detections included 33 pesticides, including insecticides, fungicides, herbicides, degradates, and a synergist. Patterns in pesticide occurrence reveal transport of dissolved and sediment-bound pesticides, including pyrethroids, from upland areas through stormwater outfalls to receiving streams. Nearly all streams contained at least one insecticide at levels exceeding an aquatic-life benchmark, most often for bifenthrin and (or) fipronil. Multiple U.S. EPA benchmark or criterion exceedances occurred in 40 % of urban streams sampled. Bed sediment concentrations of bifenthrin were highly correlated ( p  < 0.001) with benthic invertebrate assemblages. Non-insects and tolerant invertebrates such as amphipods, flatworms, nematodes, and oligochaetes dominated streams with relatively high concentrations of bifenthrin in bed sediments, whereas insects, sensitive invertebrates, and mayflies were much more abundant at sites with no or low bifenthrin concentrations. The abundance of sensitive invertebrates, % EPT, and select mayfly taxa were strongly negatively correlated with organic-carbon normalized bifenthrin concentrations in streambed sediments. Our findings from western Clackamas County, Oregon (USA), expand upon previous research demonstrating the transport of pesticides from urban landscapes and linking impaired benthic invertebrate assemblages in urban streams with exposure to pyrethroid insecticides.

Oregon↗

USGS2016

Long-term decreases in phosphorus and suspended solids, but not nitrogen, in six upper Mississippi River tributaries, 1991–2014

Long-term trends in tributaries provide valuable information about temporal changes in inputs of nutrients and sediments to large rivers. Data collected from 1991 to 2014 were used to investigate trends in total nitrogen (TN), total phosphorus (TP), nitrate (NO3–N), soluble-reactive P (SRP), and total suspended solids (TSS) in the following six tributaries of the upper Mississippi River: Cannon (CaR; Minnesota (MN)), Maquoketa (MR; Iowa (IA)), Wapsipinicon (WR; IA), Cuivre (CuR; Missouri (MO)), Chippewa (ChR; Wisconsin (WI)), and Black (BR; WI) rivers. Weighted regression on time discharge and season was used to statistically remove effects of random variation in discharge from estimated trends in flow-normalized concentrations and flux. Concentration and flux of TSS declined in all six rivers. Concentration of P declined in four of the rivers, and P flux declined in five rivers. Concentration and flux of N exhibited small changes relative to TP. TN concentration and flux did not change substantially in four of the rivers and decreased in two (ChR, CuR). Nitrate concentration and flux increased in three rivers (ChR, BR, CaR) and remained relatively constant in the other three rivers. General declines in P and TSS suggest that improvements in agricultural land management, such as the adoption of conservation tillage and enrollment of vulnerable acreage into the Conservation Reserve Program, may have reduced surface runoff; similar reductions in N were not observed.

Illinois, Iowa, Minnesota, Missouri, Wisconsin↗

USGS2017

Pre-mining trace element and radiation exposure to biota from a breccia pipe uranium mine in the Grand Canyon (Arizona, USA) watershed

The risks to wildlife and humans from uranium (U) mining in the Grand Canyon watershed are largely unknown. In addition to U, other co-occurring ore constituents contribute to risks to biological receptors depending on their toxicological profiles. This study characterizes the pre-mining concentrations of total arsenic (As), cadmium (Cd), copper (Cu), lead (Pb), mercury (Hg), nickel (Ni), selenium (Se), thallium (Tl), U, and zinc (Zn); radiation levels; and histopathology in biota (vegetation, invertebrates, amphibians, birds, and mammals) at the Canyon Mine. Gross alpha levels were below the reporting limit (4 pCi/g) in all samples, and gross beta levels were indicative of background in vegetation (<10–17 pCi/g) and rodents (<10–43.5 pCi/g). Concentrations of U, Tl, Pb, Ni, Cu, and As in vegetation downwind from the mine were likely the result of aeolian transport. Chemical concentrations in rodents and terrestrial invertebrates indicate that surface disturbance during mine construction has not resulted in statistically significant spatial differences in fauna concentrations adjacent to the mine. Chemical concentrations in egg contents and nestlings of non-aquatic birds were less than method quantification limits or did not exceed toxicity thresholds. Bioaccumulation of As, Pb, Se, Tl, and U was evident in Western spadefoot ( Spea multiplicata ) tadpoles from the mine containment pond; concentrations of As (28.9–31.4 μg/g) and Se (5.81–7.20 μg/g) exceeded toxicity values and were significantly greater than in tadpoles from a nearby water source. Continued evaluation of As and Se in biota inhabiting and forging in the mine containment pond is warranted as mining progresses.

Arizona↗

USGS2017

Changes in aquatic vegetation and floodplain land cover in the Upper Mississippi and Illinois rivers (1989–2000–2010)

Quantifying changes in the cover of river-floodplain systems can provide important insights into the processes that structure these landscapes as well as the potential consequences to the ecosystem services they provide. We examined net changes in 13 different aquatic and floodplain land cover classes using photo interpreted maps of the navigable portions of the Upper Mississippi River (UMR, above the confluence with the Ohio River) and Illinois River from 1989 to 2000 and from 2000 to 2010. We detected net decreases in vegetated aquatic area in nearly all river reaches from 1989 to 2000. The only river reaches that experienced a subsequent recovery of vegetated aquatic area from 2000 to 2010 were located in the northern portion of the UMR (above navigation pool 14) and two reaches in the Illinois River. Changes on the floodplain were dominated by urban development, which increased in nearly every river reach studied from 1989 to 2000. Agricultural lands declined in most river reaches from 2000 to 2010. The loss of agricultural land cover in the northern UMR was accompanied by increases in forest cover, whereas in the lower UMR and Illinois River, declines in agriculture were accompanied by increases in forest and shallow marsh communities. The changes in aquatic vegetation occupied between 5 and 20% of the total aquatic area and are likely associated with previously reported regional improvements in water clarity, while smaller (1–15% of the total floodplain area) changes in anthropogenic land cover types on the floodplain are likely driven by broad-scale socio-economic conditions.

Illinois River, Upper Mississippi River↗

USGS2017

Harmonization of forest disturbance datasets of the conterminous USA from 1986 to 2011

Several spatial forest disturbance datasets exist for the conterminous USA. The major problem with forest disturbance mapping is that variability between map products leads to uncertainty regarding the actual rate of disturbance. In this article, harmonized maps were produced from multiple data sources (i.e., Global Forest Change, LANDFIRE Vegetation Disturbance, National Land Cover Database, Vegetation Change Tracker, and Web-Enabled Landsat Data). The harmonization process involved fitting common class ontologies and determining spatial congruency to produce forest disturbance maps for four time intervals (1986–1992, 1992–2001, 2001–2006, and 2006–2011). Pixels mapped as disturbed for two or more datasets were labeled as disturbed in the harmonized maps. The primary advantage gained by harmonization was improvement in commission error rates relative to the individual disturbance products. Disturbance omission errors were high for both harmonized and individual forest disturbance maps due to underlying limitations in mapping subtle disturbances with Landsat classification algorithms. To enhance the value of the harmonized disturbance products, we used fire perimeter maps to add information on the cause of disturbance.

Environmental Monitoring and Assessment↗

USGS2017

Relative performance of three stream bed stability indices as indicators of stream health

Bed stability is an important stream habitat attribute because it affects geomorphology and biotic communities. Natural resource managers desire indices of bed stability that can be used under a wide range of geomorphic conditions, are biologically meaningful, and are easily incorporated into sampling protocols. To eliminate potential bias due to presence of instream wood and increase precision of stability values, we modified a stream bed instability index (ISI) to include measurements of bankfull depth ( d bf ) and median particle diameter ( D 50 ) only in riffles and increased the pebble count to decrease variability (i.e., increase precision) in D 50 . The new riffle-based instability index (RISI) was compared to two established indices: ISI and the riffle stability index (RSI). RISI and ISI were strongly associated with each other but neither was closely associated with RSI. RISI and ISI were closely associated with both a diatom- and two macrovertebrate-based stream health indices, but RSI was only weakly associated with the macroinvertebrate indices. Unexpectedly, precision of D 50 did not differ between RISI and ISI. Results suggest that RISI is a viable alternative to both ISI and RSI for evaluating bed stability in multiple stream types. With few data requirements and a simple protocol, RISI may also better conform to riffle-based sampling methods used by some water quality practitioners.

Montana↗

USGS2017

Methodological considerations of terrestrial laser scanning for vegetation monitoring in the sagebrush steppe

Terrestrial laser scanning (TLS) provides fast collection of high-definition structural information, making it a valuable field instrument to many monitoring applications. A weakness of TLS collections, especially in vegetation, is the occurrence of unsampled regions in point clouds where the sensor’s line-of-sight is blocked by intervening material. This problem, referred to as occlusion, may be mitigated by scanning target areas from several positions, increasing the chance that any given area will fall within the scanner’s line-of-sight from at least one position. Because TLS collections are often employed in remote regions where the scope of sampling is limited by logistical factors such as time and battery power, it is important to design field protocols which maximize efficiency and support increased quantity and quality of the data collected. This study informs researchers and practitioners seeking to optimize TLS sampling methods for vegetation monitoring in dryland ecosystems through three analyses. First, we quantify the 2D extent of occluded regions based on the range from single scan positions. Second, we measure the efficacy of additional scan positions on the reduction of 2D occluded regions (area) using progressive configurations of scan positions in 1 ha plots. Third, we test the reproducibility of 3D sampling yielded by a 5-scan/ha sampling methodology using redundant sets of scans. Analyses were performed using measurements at analysis scales of 5 to 50 cm across the 1-ha plots, and we considered plots in grass and shrub-dominated communities separately. In grass-dominated plots, a center-scan configuration and 5 cm pixel size sampled at least 90% of the area up to 18 m away from the scanner. In shrub-dominated plots, sampling at least 90% of the area was only achieved within a distance of 12 m. We found that 3 and 5 scans/ha are needed to sample at least ~ 70% of the total area (1 ha) in the grass and shrub-dominated plots, respectively, using 5 cm pixels to measure sampling presence-absence. The reproducibility of 3D sampling provided by a 5 position scan layout across 1-ha plots was 50% (shrub) and 70% (grass) using a 5-cm voxel size, whereas at the 50-cm voxel scale, reproducibility of sampling was nearly 100% for all plot types. Future studies applying TLS in similar dryland environments for vegetation monitoring may use our results as a guide to efficiently achieve sampling coverage and reproducibility in datasets.

Idaho↗

USGS2018

Annual variation in polychlorinated biphenyl (PCB) exposure in tree swallow (Tachycineta bicolor) eggs and nestlings at Great Lakes Restoration Initiative (GLRI) study sites

Tree swallow ( Tachycineta bicolor ) eggs and nestlings were collected from 16 sites across the Great Lakes to quantify normal annual variation in total polychlorinated biphenyl (PCB) exposure and to validate the sample size choice in earlier work. A sample size of five eggs or five nestlings per site was adequate to quantify exposure to PCBs in tree swallows given the current exposure levels and variation. There was no difference in PCB exposure in two randomly selected sets of five eggs collected in the same year, but analyzed in different years. Additionally, there was only modest annual variation in exposure, with between 69% (nestlings) and 73% (eggs) of sites having no differences between years. There was a tendency, both statistically and qualitatively, for there to be less exposure in the second year compared to the first year.

Great Lakes↗

USGS2018

A novel high-frequency groundwater quality monitoring system

High-frequency, long-term monitoring of water quality has revolutionized the study of surface waters in recent years. However, application of these techniques to groundwater has been limited by the ability to remotely pump and analyze groundwater. This paper describes a novel autonomous groundwater quality monitoring system which samples multiple wells to evaluate temporal changes and identify trends in groundwater chemistry. The system, deployed near Fresno, California, USA, collects and transmits high-frequency data, including water temperature, specific conductance, pH, dissolved oxygen, and nitrate, from supply and monitoring wells, in real-time. The system consists of a water quality sonde and optical nitrate sensor, manifold, submersible three-phase pump, variable frequency drive, data collection platform, solar panels, and rechargeable battery bank. The manifold directs water from three wells to a single set of sensors, thereby reducing setup and operation costs associated with multi-sensor networks. Sampling multiple wells at high frequency for several years provided a means of monitoring the vertical distribution and transport of solutes in the aquifer. Initial results show short period variability of nitrate, specific conductivity, and dissolved oxygen in the shallow aquifer, while the deeper portion of the aquifer remains unchanged—observations that may be missed with traditional discrete sampling approaches. In this aquifer system, nitrate and specific conductance are increasing in the shallow aquifer, while invariant changes in deep groundwater chemistry likely reflect relatively slow groundwater flow. In contrast, systems with high groundwater velocity, such as karst aquifers, have been shown to exhibit higher-frequency groundwater chemistry changes. The stability of the deeper aquifer over the monitoring period was leveraged to develop estimates of measurement system uncertainty, which were typically lower than the manufacturer’s stated specifications, enabling the identification of subtle variability in water chemistry that may have otherwise been missed.

California↗

USGS2019

The presence of antibiotic resistance genes in coastal soil and sediment samples from the eastern seaboard of the USA

Infections from antibiotic resistant microorganisms are considered to be one of the greatest global public health challenges that result in huge annual economic losses. While genes that impart resistance to antibiotics (AbR) existed long before the discovery and use of antibiotics, anthropogenic uses of antibiotics in agriculture, domesticated animals, and humans are known to influence the prevalence of these genes in pathogenic microorganisms. It is critical to understand the role that natural and anthropogenic processes have on the occurrence and distribution of antibiotic resistance in microbial populations to minimize health risks associated with exposures. As part of this research, 15 antibiotic resistance genes were analyzed in coastal sediments and soils along the eastern seaboard of the USA using presence/absence quantitative and digital polymerase chain reaction assays. Samples (53 soil and 192 sediment samples including 54 replicates) were collected from a variety of coastal settings where human and wildlife exposure is likely. At least one of the antibiotic resistance genes was detected in 76.4% of the samples. Samples that contained at least five or more antibiotic resistance genes (5.7%) where typically hydrologically down gradient of watersheds influenced by combined sewer outfalls (CSO). The most frequently detected antibiotic resistance target genes were found in 33.2%, 34.4%, and 42.2% of samples (target genes bla SHV , tetO , and aadA2 , respectively). These data provide unique insight into potential exposure of AbR genes over a large geographical region of the eastern seaboard of the USA.

Connecticut, Delaware, District of Columbia, Maine↗

USGS2020

Predicting microcystin concentration action-level exceedances resulting from cyanobacterial blooms in selected lake sites in Ohio

Cyanobacterial harmful algal blooms and the toxins they produce are a global water-quality problem. Monitoring and prediction tools are needed to quickly predict cyanotoxin action-level exceedances in recreational and drinking waters used by the public. To address this need, data were collected at eight locations in Ohio, USA, to identify factors significantly related to observed concentrations of microcystins (a freshwater cyanotoxin) that could be used in two types of site-specific regression models. Real-time models include easily- or continuously-measured factors that do not require that a sample be collected; comprehensive models use a combination of discrete sample-based measurements and real-time factors. The study sites included two recreational sites and six water treatment plant sites. Real-time models commonly included variables such as phycocyanin, pH, specific conductance, and streamflow or gage height. Many real-time factors were averages over time periods antecedent to the time the microcystin sample was collected, including water-quality data compiled from continuous monitors. Comprehensive models were useful at some sites with lagged variables for cyanobacterial toxin genes, dissolved nutrients, and (or) nitrogen to phosphorus ratios. Because models can be used for management decisions, important measures of model performance were sensitivity, specificity, and accuracy of estimates above or below the microcystin concentration threshold standard or action level. Sensitivity is how well the predictive tool correctly predicts exceedance of a threshold, an important measure for water-resource managers. Sensitivities >90% at four Lake Erie water treatment plants indicated that models with continuous monitor data were especially promising. The planned next steps are to collect more data to build larger site-specific datasets and validate models before they can be used for management decisions.

Ohio↗

USGS2020

Mercury, cadmium, copper, arsenic, and selenium measurements in the feathers of adult eastern brown pelicans (Pelecanus occidentalis carolinensis) and chicks in multiple breeding grounds in the northern Gulf of Mexico

The amounts of trace metals and metalloids that have been introduced into aquatic ecosystems due to anthropogenic activities have increased in recent decades. Some of these elements like mercury are easily transferred from one trophic level to another and can accumulate to toxic quantities in organisms at the top of aquatic food webs. For this reason, seabirds like the Eastern brown pelican (Pelecanus occidentalis carolinensis ) are susceptible to heavy metal and metalloid toxicity and may warrant periodic monitoring. Mercury, cadmium, copper, arsenic and selenium were measured in the feathers of adult brown pelicans and chicks in several breeding colonies (Shamrock Island, Chester Island, Marker 52 Island, North Deer Island, Raccoon Island, Felicity Island, Gaillard Island, Audubon Island, and Ten Palms Island) in the Northern Gulf of Mexico. Overall, most chicks and adults examined had mercury levels in feathers that were below the concentration range in which birds show symptoms of mercury toxicity. However, chicks in the Audubon Island and Ten Palms Island colonies displayed mercury levels that were 3 times higher than values observed in 5 other colonies. In addition, several adults and chicks displayed selenium concentrations that are above what is considered safe for birds. Cadmium quantities in feathers were below levels that trigger toxicity in birds. Similarly, arsenic measurements were at quantities below the average of what has been reported for birds living in contaminated sites. Finally, we identify pelican breeding colonies that may warrant monitoring due to elevated levels of contaminants.

Alabama, Florida, Louisiana,Texas↗

USGS2023

Aquatic insect accumulation of uranium at spring outflows in the Grand Canyon region as influenced by aqueous and sediment geochemistry and biological factors: Implications for monitoring

Potential adverse ecological effects of expanded uranium (U) mining within the Grand Canyon region motivated studies to better understand U exposure and risk to endemic species. This study documents U exposures and analyzes geochemical and biological factors affecting U bioaccumulation at spring-fed systems within the Grand Canyon region. The principal objective was to determine if aqueous U was broadly indicative of U accumulated by insect larvae, a dominate fauna. Analyses focused on three widely distributed taxa: Argia sp. (a predatory damselfly), Culicidae (suspension feeding mosquitos), and Limnephilus sp. (a detritivorous caddisfly). The study showed that U accumulated by aquatic insects (and periphyton) generally correlated positively with total dissolved U, although correlations were strongest when based on modeled concentrations of the U-dicarbonato complex, UO 2 (CO 3 ) 2 –2 , and UO 2 (OH) 2 . Sediment metal concentration was a redundant indicator of U bioaccumulation. Neither insect size or U in the gut content of Limnephilus sp. substantially affected correlations between aqueous U and whole-body U concentrations. However, in Limnephilus sp., the gut and its content contained large quantities of U. Estimates of the sediment burden in the gut indicated that sediment was a minor source of U mass but contributed substantially to the total insect weight. As a result, whole-body U concentration would tend to vary inversely with the sediment burden of the gut. The correlations between aqueous U and bioaccumulated U provide an initial relational baseline against which newly acquired data could be evaluated for changes in U exposure during and after mining operations.

Arizona↗

USGS2023

Prevalence of Ophidiomyces ophidiicola and epizootiology of snake fungal disease in free-ranging Northern Pine Snakes (Pituophis melanoleucus melanoleucus) in New Jersey

Snake fungal disease, caused by Ophidiomyces ophidiicola , is recognized as a potential concern for North American snakes. We tested skin swabs from Northern Pine Snakes ( Pituophis melanoleucus melanoleucus ) in the New Jersey pinelands for the presence of O. ophidiicola before emergence from hibernation. We used qPCR to test the collected swabs for the presence of O. ophidiicola , then determined pathogen prevalence as a function of sampling year, sampling location (skin lesion, healthy ventral skin, healthy head skin) sex, and age . There were no temporal trends in O. ophidiicola detection percentages on snakes, which varied from 58 to 83% in different years. Ophidiomyces ophidiicola detection on snakes was highest in swabs of skin lesions (71%) and lowest in head swabs (29%). Males had higher prevalence than females (82% versus 62%). The fungus was not detected in hatchling snakes (age 0) in the fall, but 75% of juveniles tested positive at the end of hibernation (age 1 year). We also screened hibernacula soil samples for the presence of O. ophidiicola . Where snakes hibernated, 69% of soil samples were positive for O. ophidiicola , and 85% of snakes lying on positive soil samples also tested positive for the pathogen. Although a high proportion of snakes (73%) tested positive for O. ophidiicola during our 4-year study , the snakes appeared healthy except for small skin lesions. We conclude that O. ophidiicola prevalence is high on hibernating Northern Pine Snakes and in the hibernacula soil, with a strong association between snakes and positive adjacent soil. This is the first demonstration that snakes likely become infected during hibernation.

New Jersey↗