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Information resources

During recent decades, natural resources agency personnel and others involved with the management and stewardship of wildlife have experienced an increasing need to access information and obtain technical assistance for addressing a diverse array of wildlife disease issues. This Chapter provides a broad overview of selected sources for obtaining supplemental information and technical assistance for addressing wildlife disease issues in North America. Specifically, examples of existing major wildlife disease programs focusing on free-ranging wildlife populations are highlighted; training opportunities for enhancing within-agency wildlife disease response are identified; a selected reading list of wildlife disease references is provided; and selected Web sites providing timely information on wildlife disease are highlighted. No attempt is made to detail all the North American programs and capabilities that address disease in free-ranging wildlife populations. Instead, this Chapter is focused on enhancing awareness of the types of capabilities that exist as potential sources for assistance and collaboration between wildlife conservation agency personnel and others in addressing wildlife disease issues. A wide variety of entities across North America are involved in wildlife disease investigations; however, the formal assembly of multidimensional programs that primarily address disease for the benefit of free-ranging wildlife is rather limited. The Southeastern Cooperative Wildlife Disease Study (SCWDS), the National Wildlife Health Center (NWHC), and the Canadian Wildlife Health Cooperative (CWHC) are selected examples. These programs are highlighted because of the scope of their capabilities and long-term involvement in assisting State and Federal natural resource agencies combat wildlife disease. A variety of other sources for possible assistance in addressing wildlife disease issues exists throughout North America and globally. It is prudent for wildlife conservation field biologists, managers, and administrators to be aware of such local resources. Ideally, awareness and knowledge of the types of assistance those programs can provide should be obtained prior to disease crisis events since appropriate, timely intervention often is required to minimize wildlife losses from disease and prevent the establishment of new infectious diseases within wildlife populations and geographic areas. Increasing recognition of the substantial number of infectious diseases being transferred between wildlife, domestic animals, and humans has led to increased collaborative investigations between wildlife, domestic, and human health programs. That collaboration has led to a heightened focus on wildlife disease within some public health and agriculture agencies. For purposes of this Chapter, wildlife disease is narrowly defined as those diseases (infectious and noninfectious) causing morbidity and mortality in free-ranging wildlife populations. Therefore, there is no focus on the numerous fish disease or environmental contaminant programs that exist on behalf of North American fauna.

Techniques and Methods

Report on the 2010 Chilean earthquake and tsunami response

In July 2010, in an effort to reduce future catastrophic natural disaster losses for California, the American Red Cross coordinated and sent a delegation of 20 multidisciplinary experts on earthquake response and recovery to Chile. The primary goal was to understand how the Chilean society and relevant organizations responded to the magnitude 8.8 Maule earthquake that struck the region on February 27, 2010, as well as how an application of these lessons could better prepare California communities, response partners and state emergency partners for a comparable situation. Similarities in building codes, socioeconomic conditions, and broad extent of the strong shaking make the Chilean earthquake a very close analog to the impact of future great earthquakes on California. To withstand and recover from natural and human-caused disasters, it is essential for citizens and communities to work together to anticipate threats, limit effects, and rapidly restore functionality after a crisis. The delegation was hosted by the Chilean Red Cross and received extensive briefings from both national and local Red Cross officials. During nine days in Chile, the delegation also met with officials at the national, regional, and local government levels. Technical briefings were received from the President’s Emergency Committee, emergency managers from ONEMI (comparable to FEMA), structural engineers, a seismologist, hospital administrators, firefighters, and the United Nations team in Chile. Cities visited include Santiago, Talca, Constitución, Concepción, Talcahuano, Tumbes, and Cauquenes. The American Red Cross Multidisciplinary Team consisted of subject matter experts, who carried out special investigations in five Teams on the (1) science and engineering findings, (2) medical services, (3) emergency services, (4) volunteer management, and (5) executive and management issues (see appendix A for a full list of participants and their titles and teams). While developing this delegation, it was clear that a multidisciplinary approach was required to properly analyze the emergency response, technical, and social components of this disaster. A diverse and knowledgeable delegation was necessary to analyze the Chilean response in a way that would be beneficial to preparedness in California, as well as improve mitigation efforts around the United States. By most standards, the Maule earthquake was a catastrophe for Chile. The economic losses totaled $30 billion USD or 17% of the GDP of the country. Twelve million people, or ¾ of the population of the country, were in areas that felt strong shaking. Yet only 521 fatalities have been confirmed, with 56 people still missing and presumed dead in the tsunami. The Science and Technology Team evaluated the impacts of the earthquake on built environment with implications for the United States. The fires following the earthquake were minimal in part because of the shutdown of the national electrical grid early in the shaking. Only five engineer-designed buildings were destroyed during the earthquake; however, over 350,000 housing units were destroyed. Chile has a law that holds building owners liable for the first 10 years of a building’s existence for any losses resulting from inadequate application of the building code during construction. This law was cited by many our team met with as a prime reason for the strong performance of the built environment. Overall, this earthquake demonstrated that strict building codes and standards could greatly reduce losses in even the largest earthquakes. In the immediate response to the earthquake and tsunami, first responders, emergency personnel, and search and rescue teams handled many challenges. Loss of communications was significant; many lives were lost and effective coordination to support life-sustaining efforts was gravely impacted due to a lack of inter- and intra-agency coordination. The Health and Medical Services Team sought to understand the medical disaster response strategies and operations of Chilean agencies, including perceived or actual failures in disaster preparation that impacted the medical disaster response; post-disaster health and medical interventions to save lives and limit suffering; and the lessons learned by public health and medical personnel as a result of their experiences. Despite devastating damage to the health care and civic infrastructure, the health care response to the Chilean earthquake appeared highly successful due to several factors. Like other first responders, the medical community had the ability and resourcefulness to respond without centralized control in the early response phase. The health care community maintained patient care under austere conditions, despite many obstacles that could have prevented such care. National and international resources were rapidly mobilized to support the medical response. The Emergency Services Team sought to collect information on all phases of emergency management (preparedness, mitigation, response, and recovery) and determine what worked well and what could be improved upon. The Chileans reported being surprised that they were not as ready for this event as they thought they were. The use of mass care sheltering was limited, given the scope of the disaster, because of the resiliency of the population. The impacts of the earthquake and the tsunami were quite different, as were the needs of urban and rural dwellers, necessitating different response activities. The Volunteer Services Team examined the challenges faced in mobilizing a large number of volunteers to assist in the aftermath of a disaster of this scale. One of the greatest challenges expressed was difficulty in communication; the need for redundancy in communication mechanisms was cited. The flexibility and ability to work autonomously by the frontline volunteers was a significant factor in effective response. It was also important for volunteer leadership to know the emergency plans. These plans need to be flexible, include alternative options, and be completed in conjunction with local officials and other volunteers. The Executive/Red Cross Management Team took a broad look at the impacts of the earthquake and the implications for California. Some of the most important preparation for the disaster came from relationships formed before the event. The communities with strong connections between different government services generally fared well. The initial response and resilience of individuals and communities was another important component. Communication system failures limited the ability of a central government to assist impacted communities, or to issue tsunami warnings. It also delayed the response since the government did not know (in some case for several days) the impact and needs of local governments. In general, plans for congregate care shelters existed but were little used as most people chose to stay at damaged homes or with relatives. Looting was a surprise to response officials as well as social scientists, but both public and private sector organizations, including NGOs (Non-Governmental Organizations), must consider security for damaged businesses as a priority in California’s multihazard planning. Class and ethnic divisions that become heightened during some cases of actual or perceived injustice may also emerge in natural disasters in California. Several factors contributed overall to the low casualty rate and rapid recovery. A major factor is the strong building code in Chile and its comprehensive enforcement. In particular, Chile has a law that holds building owners accountable for losses in a building they build for 10 years. A second factor was the limited number of fires after the earthquake. In the last few California earthquakes, 60% of the fires were started by electrical problems, so the rarity of fires may have been affected by the shut down of the electricity grid early in the earthquake. Third, in many areas, the local emergency response was very effective. The most effective regions had close coordination between emergency management, fire, and police and were empowered to respond without communication with the capital. The fourth factor was the overall high level of knowledge about earthquakes and tsunamis by much of the population that helped them respond more appropriately after the event.

Open-File Report

Groundwater quality in the Owens Valley, California

Groundwater provides more than 40 percent of California’s drinking water. To protect this vital resource, the State of California created the Groundwater Ambient Monitoring and Assessment (GAMA) Program. The Priority Basin Project of the GAMA Program provides a comprehensive assessment of the State’s groundwater quality and increases public access to groundwater-quality information. Owens Valley is one of the study areas being evaluated. The Owens study area is approximately 1,030 square miles (2,668 square kilometers) and includes the Owens Valley groundwater basin (California Department of Water Resources, 2003). Owens Valley has a semiarid to arid climate, with average annual rainfall of about 6 inches (15 centimeters). The study area has internal drainage, with runoff primarily from the Sierra Nevada draining east to the Owens River, which flows south to Owens Lake dry lakebed at the southern end of the valley. Beginning in the early 1900s, the City of Los Angeles began diverting the flow of the Owens River to the Los Angeles Aqueduct, resulting in the evaporation of Owens Lake and the formation of the current Owens Lake dry lakebed. Land use in the study area is approximately 94 percent (%) natural, 5% agricultural, and 1% urban. The primary natural land cover is shrubland. The largest urban area is the city of Bishop (2010 population of 4,000). Groundwater in this basin is used for public and domestic water supply and for irrigation. The main water-bearing units are gravel, sand, silt, and clay derived from surrounding mountains. Recharge to the groundwater system is primarily runoff from the Sierra Nevada, and by direct infiltration of irrigation. The primary sources of discharge are pumping wells, evapotranspiration, and underflow to the Owens Lake dry lakebed. The primary aquifers in Owens Valley are defined as those parts of the aquifers corresponding to the perforated intervals of wells listed in the California Department of Public Health database. Public-supply wells in Owens Valley are completed to depths between 210 and 480 feet (64 to 146 meters), consist of solid casing from the land surface to a depth of 50 to 80 feet (15 to 24 meters), and are screened or perforated below the solid casing.

California

The roles of antimicrobial resistance, phage diversity, isolation source, and selection in shaping the genomic architecture of Bacillus anthracis

Bacillus anthracis, the causative agent of anthrax disease, is a worldwide threat to livestock, wildlife and public health. While analyses of genetic data from across the globe have increased our understanding of this bacterium’s population genomic structure, the influence of selective pressures on this successful pathogen is not well understood. In this study, we investigate the effects of antimicrobial resistance, phage diversity, geography and isolation source in shaping population genomic structure. We also identify a suite of candidate genes potentially under selection, driving patterns of diversity across 356 globally extant B. anthracis genomes. We report ten antimicrobial resistance genes and 11 different prophage sequences, resulting in the first large-scale documentation of these genetic anomalies for this pathogen. Results of random forest classification suggest genomic structure may be driven by a combination of antimicrobial resistance, geography and isolation source, specific to the population cluster examined. We found strong evidence that a recombination event linked to a gene involved in protein synthesis may be responsible for phenotypic differences between comparatively disparate populations. We also offer a list of genes for further examination of B. anthracis evolution, based on high-impact single nucleotide polymorphisms (SNPs) and clustered mutations. The information presented here sheds new light on the factors driving genomic structure in this notorious pathogen and may act as a road map for future studies aimed at understanding functional differences in terms of B. anthracis biogeography, virulence and evolution.

Microbial Genomics

Groundwater quality in the Indian Wells Valley, California

Groundwater provides more than 40 percent of California’s drinking water. To protect this vital resource, the State of California created the Groundwater Ambient Monitoring and Assessment (GAMA) Program. The Priority Basin Project of the GAMA Program provides a comprehensive assessment of the State’s groundwater quality and increases public access to groundwater-quality information. Indian Wells Valley is one of the study areas being evaluated. The Indian Wells study area is approximately 600 square miles (1,554 square kilometers) and includes the Indian Wells Valley groundwater basin (California Department of Water Resources, 2003). Indian Wells Valley has an arid climate and is part of the Mojave Desert. Average annual rainfall is about 6 inches (15 centimeters). The study area has internal drainage, with runoff from the surrounding mountains draining towards dry lake beds in the lower parts of the valley. Land use in the study area is approximately 97.0 percent (%) natural, 0.4% agricultural, and 2.6% urban. The primary natural land cover is shrubland. The largest urban area is the city of Ridgecrest (2010 population of 28,000). Groundwater in this basin is used for public and domestic water supply and for irrigation. The main water-bearing units are gravel, sand, silt, and clay derived from the Sierra Nevada to the west and from the other surrounding mountains. Recharge to the groundwater system is primarily runoff from the Sierra Nevada and to the west and from the other surrounding mountains. Recharge to the groundwater system is primarily runoff from the Sierra Nevada and direct infiltration from irrigation and septic systems. The primary sources of discharge are pumping wells and evapotranspiration near the dry lakebeds. The primary aquifers in the Indian Wells study area are defined as those parts of the aquifers corresponding to the perforated intervals of wells listed in the California Department of Public Health database. Public-supply wells in Indian Wells Valley are completed to depths between 240 and 800 feet (73 to 244 meters), consist of solid casing from the land surface to a depth of 180 to 260 feet (55 to 79 meters), and are screened or perforated below the solid casing.

California

Using the value of information to improve conservation decision making

Conservation decisions are challenging, not only because they often involve difficult conflicts among outcomes that people value, but because our understanding of the natural world and our effects on it is fraught with uncertainty. Value of Information (VoI) methods provide an approach for understanding and managing uncertainty from the standpoint of the decision maker. These methods are commonly used in other fields (e.g., economics, public health) and are increasingly used in biodiversity conservation. This decision analytical approach can identify the best management alternative to select where the effectiveness of interventions is uncertain, and can help to decide when to act and when to delay action until after further research. We review the use of VoI in the environmental domain, reflect on the need for greater uptake of VoI, particularly for strategic conservation planning, and suggest promising areas for new research. We also suggest common reporting standards as a means of increasing the leverage of this powerful tool. The environmental science, ecology and biodiversity categories of the Web of Knowledge were searched using the terms ‘Value of Information,’ ‘Expected Value of Perfect Information,’ and the abbreviation ‘EVPI.’ Google Scholar was searched with the same terms, and additionally the terms decision and biology, biodiversity conservation, fish, or ecology. We identified 1225 papers from these searches. Included studies were limited to those that show an application of VoI in biodiversity conservation rather than simply describing the method. All examples of use of VOI were summarised regarding the application of VoI, the management objectives, the uncertainties, models used, how the objectives were measured, and the type of VoI. While the use of VoI appears to be on the increase in biodiversity conservation, the reporting of results is highly variable, which can make it difficult to understand the decision context and which uncertainties were considered. Moreover, it was unclear if, and how, the papers informed management and policy interventions, which is why we suggest a range of reporting standards that would aid the use of VoI. The use of VoI in conservation settings is at an early stage. There are opportunities for broader applications, not only for species-focussed management problems, but also for setting local or global research priorities for biodiversity conservation, making funding decisions, or designing or improving protected area networks and management. The long-term benefits of applying VoI methods to biodiversity conservation include a more structured and decision-focused allocation of resources to research.

Biological Reviews

Forest management and bats

Because more than half of the forest land in the United States is privately owned, forest landowners play an important role in the stewardship of our wildlife resources. This publication will introduce you to a group of wildlife that is particularly important to forest ecosystems, but also one of the most misunderstood: bats. We will demonstrate how active forest management can improve forest health and productivity while maintaining and enhancing habitat for these fascinating and beneficial mammals.

Report

Status and understanding of groundwater quality in the Madera, Chowchilla Study Unit, 2008: California GAMA Priority Basin Project

Groundwater quality in the approximately 860-square-mile Madera and Chowchilla Subbasins (Madera-Chowchilla study unit) of the San Joaquin Valley Basin was investigated as part of the Priority Basin Project of the Groundwater Ambient Monitoring and Assessment (GAMA) Program. The study unit is located in California's Central Valley region in parts of Madera, Merced, and Fresno Counties. The GAMA Priority Basin Project is being conducted by the California State Water Resources Control Board in collaboration with the U.S. Geological Survey (USGS) and the Lawrence Livermore National Laboratory. The Project was designed to provide statistically robust assessments of untreated groundwater quality within the primary aquifer systems in California. The primary aquifer system within each study unit is defined by the depth of the perforated or open intervals of the wells listed in the California Department of Public Health (CDPH) database of wells used for municipal and community drinking-water supply. The quality of groundwater in shallower or deeper water-bearing zones may differ from that in the primary aquifer system; shallower groundwater may be more vulnerable to contamination from the surface. The assessments for the Madera-Chowchilla study unit were based on water-quality and ancillary data collected by the USGS from 35 wells during April-May 2008 and water-quality data reported in the CDPH database. Two types of assessments were made: (1) status , assessment of the current quality of the groundwater resource, and (2) understanding , identification of natural factors and human activities affecting groundwater quality. The primary aquifer system is represented by the grid wells, of which 90 percent (%) had depths that ranged from about 200 to 800 feet (ft) below land surface and had depths to the top of perforations that ranged from about 140 to 400 ft below land surface. Relative-concentrations (sample concentrations divided by benchmark concentrations) were used for evaluating groundwater quality for those constituents that have Federal or California regulatory or non-regulatory benchmarks for drinking-water quality. A relative-concentration (RC) greater than 1.0 indicates a concentration above a benchmark. RCs for organic constituents (volatile organic compounds and pesticides) and special-interest constituents (perchlorate) were classified as "high" (RC is greater than 1.0), "moderate" (RC is less than or equal to 1.0 and greater than 0.1), or "low" (RC is less than or equal to 0.1). For inorganic constituents (major and minor ions, trace elements, nutrients, and radioactive constituents), the boundary between low and moderate RCs was set at 0.5. The assessments characterize untreated groundwater quality, not the quality of treated drinking water delivered to consumers by water purveyors; drinking-water benchmarks, and thus relative-concentrations, are used to provide context for the concentrations of constituents measured in groundwater. Aquifer-scale proportion was used in the status assessment as the primary metric for evaluating regional-scale groundwater quality. High aquifer-scale proportion is defined as the percentage of the area of the primary aquifer system with RCs greater than 1.0 for a particular constituent or class of constituents; moderate and low aquifer-scale proportions are defined as the percentages of the area of the primary aquifer system with moderate and low RCs, respectively. Percentages are based on an areal, rather than a volumetric basis. Two statistical approaches--grid-based, which used one value per grid cell, and spatially weighted, which used multiple values per grid cell--were used to calculate aquifer-scale proportions for individual constituents and classes of constituents. The spatially weighted estimates of high aquifer-scale proportions were within the 90% confidence intervals of the grid-based estimates for all constituents except iron. The status assessment showed that inorganic constituents had greater high and moderate aquifer-scale proportions in the Madera-Chowchilla study unit than did organic constituents. RCs for inorganic constituents with health-based benchmarks were high in 37% of the primary aquifer system, moderate in 30%, and low in 33%. The inorganic constituents contributing most to the high aquifer-scale proportion were arsenic (13%), uranium (17%), gross alpha particle activity (20%), nitrate (6.7%), and vanadium (3.3%). RCs for inorganic constituents with non-health-based benchmarks were high in 6.7% of the primary aquifer system, and the constituent contributing most to the high aquifer-scale proportion was total dissolved solids (TDS). RCs for organic constituents with health-based benchmarks were high in 10% of the primary aquifer system, moderate in 3.3%, and low in 40%; organic constituents were not detected in 47% of the primary aquifer system. The fumigant 1,2-dibromo-3-chloropropane (DBCP) was the only organic constituent detected at high RCs. Seven organic constituents were detected in 10% or more of the primary aquifer system: DBCP; the fumigant additive 1,2,3-trichloropropane; the herbicides simazine, atrazine, and diuron; the trihalomethane chloroform; and the solvent tetrachloroethene (PCE). RCs for the special-interest constituent perchlorate were moderate in 20% of the primary aquifer system. The second component of this study, the understanding assessment , identified the natural and human factors that may affect groundwater quality by evaluating statistical correlations between water-quality constituents and potential explanatory factors, such as land use, position relative to important geologic features, groundwater age, well depth, and geochemical conditions in the aquifer. Results of the statistical evaluations were used to explain the distribution of constituents in the study unit. Depth to the top of perforations in the well and groundwater age were the most important explanatory factors for many constituents. High and moderate RCs of nitrate, uranium, and TDS and the presence of herbicides, trihalomethanes, and solvents were all associated with depths to the top of perforations less than 235 ft and modern- and mixed-age groundwater. Positive correlations between uranium, bicarbonate, TDS, and the proportion of calcium and magnesium in the total cations suggest that downward movement of recharge from irrigation water contributed to the elevated concentrations of these constituents in the primary aquifer system. High and moderate RCs of arsenic were associated with depths to the top of perforations greater than 235 ft, mixed- and pre-modern-age groundwater, and location in sediments from the Chowchilla River alluvial fan, suggesting that increased residence time and appropriate aquifer materials were needed for arsenic to accumulate in the groundwater. High and moderate RCs of fumigants were associated with depths to the top of perforations of less than 235 ft and location south of the city of Madera; low RCs of fumigants were detected in wells dispersed across the study unit with a range of depths to top of perforations.

California

Emergence of a zoonotic pathogen in a coastal marine sentinel: Capillaria hepatica (syn. Calodium hepaticum)-associated hepatitis in southern sea otters (Enhydra lutris nereis)

Capillaria hepatica is a globally distributed zoonotic nematode parasite that most commonly infects feral and native rats. Soil contact, pica, and living in close proximity to rat populations are risk factors for human infection. Larval nematodes and eggs that were morphologically consistent with C. hepatica were observed microscopically in livers of stranded southern sea otters ( Enhydra lutris nereis ) from California. Large (90–100 × 45–55 μm), barrel-shaped non-embryonated parasite eggs with large polar prominences and a roughened or striated surface, or 105–120 μm diameter larval aphasmid nematode profiles with a prominent stichosome and hypodermal bands were observed in the livers of three otters. The liver of a fourth animal exhibited serpiginous tracts of necrosis, micro-cavitation and pleocellular inflammation, with intralesional linear eosinophilic material that resembled cuticle from degenerating metazoan parasites. Capillaria hepatica -associated hepatitis and capsular adhesions were the cause of death for one otter, and parasite-associated liver lesions were observed in all cases. All infected otters were adult females that stranded from 2006 through 2016 at multiple sites along the central California coast. All cases stranded from December through May; during and soon after peak seasonal precipitation and land-sea runoff in California. This same seasonal pattern has been reported for other land-based parasites infecting southern sea otters. Neither C. hepatica , nor any similar nematodes have been reported from marine mammals, and southern sea otters are not typical hosts for C. hepatica or any other nematode parasites. The most likely route of exposure was via freshwater runoff containing embryonated eggs liberated from predated or decomposing terrestrial hosts, especially rats. Similar to the land-based parasites Toxoplasma gondii and Sarcocystis neurona , C. hepatica eggs may be concentrated and transmitted through filter-feeding marine invertebrates that serve as southern sea otter prey, which may also pose an unrecognized public health risk for people who consume these species.

California

Investigation of potential factors controlling benthic algae in the upper White River Basin, Colorado, 2018–21

Nuisance levels of benthic filamentous green algae are becoming increasingly common in surface waters of Colorado and the western United States. In 2018 the U.S. Geological Survey began a study in cooperation with the White River and Douglas Creek Conservation Districts, Colorado River Basin Salinity Control Forum, and the Colorado River Water Conservation District to collect and analyze physical, chemical, and biological information for the upper White River Basin in Colorado and investigate causes of benthic algal blooms in the basin. This report (1) presents site-specific data including water temperature, riparian canopy cover, streambed particle size, and algal biomass and community composition; (2) describes the potential for streambed movement during spring runoff using physical channel characteristics and peak streamflow velocities; and (3) explains the results of a linear mixed-effects model used to test hypotheses about the influence of physical and chemical factors in explaining the occurrence of algal blooms across the basin. Benthic algal biomass ranged from 0.7 to 309 milligrams per square meter during the summer (July–August) from 2018 through 2021 and exceeded the Colorado Department of Public Health and Environment criteria of 150 milligrams per square meter on four occasions, in 2018. Four genera of filamentous green algae were identified in the upper White River Basin, including Cladophora , Stigeoclonium , Ulothrix , and Spirogyra . Many genera of cyanobacteria were present, including some capable of producing toxins and taste and odor compounds. The nuisance diatom Didymosphenia geminata , commonly referred to as didymo, was found at two sites on the South Fork White River and along the main stem White River. Hypotheses pertaining to the influence of measured variables on algal biomass were tested with a linear mixed-effects model. Median rock size and mean August water temperature had significant positive effects, meaning that greater bed stability and higher mean August water temperatures result in greater algal biomass. Total nitrogen to total phosphorus ratios had a significant negative effect on algal biomass, meaning that more nitrogen-limiting conditions, or greater phosphorus availability, corresponded to greater algal biomass. Streamflow and water temperature data at White River above Coal Creek near Meeker, Colo., were used to assess possible causes of bloom conditions across years, including when algal blooms were first studied in the basin during 2016 and 2017. Early or low-magnitude peak streamflow conditions were not prerequisites for algal bloom occurrence. Conversely, relatively large, late, and long-lasting peak streamflows, such as those measured in 2019, may limit algal blooms during the same year and into subsequent years, as evidenced by extremely low algal biomass in 2019 and 2020. The broad spatial extent of bloom conditions indicates that the factors contributing to the occurrence of algal blooms are likely basinwide. Findings from this multiyear study indicate that the effects caused by larger peak streamflow, including movement of the streambed, may be the dominant control on the occurrence of an algal bloom. The findings also indicate that in the absence of disturbance other resources, including substrate size, water temperature, and nutrient availability, moderate algal biomass.

Colorado

Ground-water quality data in the coastal Los Angeles Basin study unit, 2006: Results from the California GAMA Program

Ground-water quality in the approximately 860 square-mile Coastal Los Angeles Basin study unit (CLAB) was investigated from June to November of 2006 as part of the Statewide Basin Assessment Project of the Ground-Water Ambient Monitoring and Assessment (GAMA) Program. The GAMA Statewide Basin Assessment was developed in response to the Ground-Water Quality Monitoring Act of 2001, and is being conducted by the U.S. Geological Survey (USGS) in cooperation with the California State Water Resources Control Board (SWRCB). The Coastal Los Angeles Basin study was designed to provide a spatially unbiased assessment of raw ground-water quality within CLAB, as well as a statistically consistent basis for comparing water quality throughout California. Samples were collected from 69 wells in Los Angeles and Orange Counties. Fifty-five of the wells were selected using a spatially distributed, randomized grid-based method to provide statistical representation of the study area (?grid wells?). Fourteen additional wells were selected to evaluate changes in ground-water chemistry or to gain a greater understanding of the ground-water quality within a specific portion of the Coastal Los Angeles Basin study unit ('understanding wells'). Ground-water samples were analyzed for: a large number of synthetic organic constituents [volatile organic compounds (VOCs), gasoline oxygenates and their degradates, pesticides, polar pesticides, and pesticide degradates, pharmaceutical compounds, and potential wastewater-indicators]; constituents of special interest [perchlorate, N-nitrosodimethylamine (NDMA), 1,4-dioxane, and 1,2,3-trichloropropane (1,2,3-TCP)]; inorganic constituents that can occur naturally [nutrients, major and minor ions, and trace elements]; radioactive constituents [gross-alpha and gross-beta radiation, radium isotopes, and radon-222]; and microbial indicators. Naturally occurring isotopes [stable isotopic ratios of hydrogen and oxygen, and activities of tritium and carbon-14] and dissolved noble gases also were measured to help identify the sources and ages of the sampled ground water. Quality-control samples (blanks, replicates, and samples for matrix spikes) were collected at approximately one-fourth of the wells, and the results for these samples were used to evaluate the quality of the data for the ground-water samples. Field blanks rarely contained detectable concentrations of any constituent, suggesting that contamination was not a significant source of bias. Differences between replicate samples were within acceptable ranges, indicating acceptably low variability. Matrix spike recoveries were within acceptable ranges for most compounds. Assessment of the quality-control information resulted in applying ?V? codes to approximately 0.1 percent of the data collected for ground-water samples (meaning a constituent was detected in blanks as well as the corresponding environmental data). This study did not attempt to evaluate the quality of drinking water delivered to consumers; after withdrawal from the ground, water typically is treated, disinfected, and (or) blended with other waters to maintain acceptable drinking-water quality. Regulatory thresholds are applied to the treated drinking water that is served to the consumer, not to raw ground water. However, to provide some context for the results, concentrations of constituents measured in the raw ground water were compared with regulatory and non-regulatory health-based thresholds established by the U.S. Environmental Protection Agency (USEPA), California Department of Public Health (CDPH, formerly California Department of Health Services [CADHS]) and thresholds established for aesthetic concerns (secondary maximum contaminant levels, SMCL-CA) by CDPH. Comparisons between data collected for this study and drinking-water thresholds are for illustrative purposes only, and are not indicative of compliance or non-compliance with those thresholds. VOCs were detected in alm

California

Water-quality comparison of the Gulf Coast aquifer system and Carrizo-Wilcox aquifer in Texas from National Water-Quality Assessment Project Principal Aquifer Surveys, 2013 and 2015

The U.S. Geological Survey’s National Water-Quality Assessment (NAWQA) Project assessed the quality of groundwater in aquifers that are important sources of drinking water in the United States. One major aquifer in Texas that was assessed by NAWQA in 2013 is the coastal lowlands aquifer system, which is often referred to in Texas as the “Gulf Coast aquifer system.” The coastal lowlands aquifer system supplies water for millions of people; self-supplied (private) well withdrawals in 2005 from this aquifer system were the sixth largest among all major aquifer systems in the Nation. A major aquifer in Texas that was assessed by NAWQA in 2015 is the Texas coastal uplands aquifer system; the Carrizo-Wilcox aquifer is one of several aquifers that compose this aquifer system in Texas. The rocks composing the Texas coastal uplands aquifer system extend east from Texas as part of the Mississippi embayment aquifer system and underlie areas of several States. The Texas coastal uplands aquifer system and Mississippi embayment aquifer system are often collectively referred to as the “Mississippi embayment-Texas coastal uplands aquifer system.” Self-supplied withdrawals from the Mississippi embayment-Texas coastal uplands aquifer system in 2005 were the eighth largest among all major aquifer systems in the Nation. The coastal lowlands aquifer system and Mississippi embayment-Texas coastal uplands aquifer system were assessed as part of the NAWQA Principal Aquifer Surveys (PAS), which were designed to evaluate constituent concentrations in water samples obtained from domestic and public-supply wells prior to any treatment. PAS assessments like these allow for the comparison of water-quality concentrations in untreated groundwater using preestablished benchmarks for the protection of human health and for aesthetic qualities such as taste, color, and odor. The use of preestablished benchmarks can provide a basis for comparison of groundwater quality among principal aquifers.

Texas

Particle atlas of World Trade Center dust

The United States Environmental Protection Agency (EPA) has begun a reassessment of the presence of World Trade Center (WTC) dust in residences, public buildings, and office spaces in New York City, New York. Background dust samples collected from residences, public buildings, and office spaces will be analyzed by multiple laboratories for the presence of WTC dust. Other laboratories are currently studying WTC dust for other purposes, such as health effects studies. To assist in inter-laboratory consistency for identification of WTC dust components, this particle atlas of phases in WTC dust has been compiled.

Open-File Report

Prevalence of Ehrlichia, Borrelia, and Rickettsial agents in Amblyomma americanum (Acari: Ixodidae) collected from nine states

Ambyomma antericanum (L.) (Acari: Ixodidae) is an aggressive tick that feeds on humans during all postembryonic life stages. In many regions of the United States, it is the tick most commonly found attached to humans. Public health interest has grown recently, due to the recognition of new human pathogens transmitted by A. antericanum and the expanding distribution of the tick. A. americanum is a vector of several bacteria pathogenic to humans. Ehrlichia chaffeensis and Ehrlichia ewingii cause moderate-to-severe febrile illness. 'Rickettsia amblyommii,' a member of the spotted fever group Rickettsia, also has recently been implicated as a possible human pathogen based on serologic evidence from persons recovering from illness after a tick bite. We have determined the prevalence of infection of Ehrlichia chaffeensis, E. ewingii, 'Borrelia lonestari,' and R. amblyommii within A. americanum ticks from 29 sites in nine states. Overall infection prevalences were 4.7% for E. chaffeensis (range, 0-27%), 3.5% for E. ewingii (range, 0-18.6%), 2.5% for B. lonestari (range, 0-12.2%), and 41.2% for R. amblyommii (range, 0-84.0%). In addition, 87 ticks (4.3%) were infected with two or more bacteria. This report documents new distribution records for E. ewingii, B. lonestari, and R. amblyommii and underscores the nonhomogeneous distribution of pathogen foci of infection. Additional surveillance throughout the range of A. antericanum is warranted to increase physician and public awareness of the risk of disease to humans from exposure to the agents transmitted by this tick.

Journal of Medical Entomology

Report from Ground Zero: How geoscientists aid in the aftermath of environmental disasters

People around the world remember when they first learned of the attacks on New York City’s World Trade Center towers on Sept. 11, 2001. For me, the memories are vivid — my feelings of shock, horror and sadness were similarly etched on the faces of all the attendees of a mining and the environment workshop in Buenos Aires, Argentina, where I was speaking. At that time, I had no idea that our small research group at the U.S. Geological Survey in Denver, Colo., would soon be called upon to help public officials understand the potential health and environmental implications of the disaster and the ensuing rescue, recovery and cleanup efforts.

Earth Magazine

Occurrence of trihalomethanes in the nation's ground water and drinking-water supply wells, 1985-2002

This report describes the occurrence of trihalomethanes (THMs) in the Nation's ground water and drinking-water supply wells based on analysis of 5,642 samples of untreated ground water and source water collected or compiled during 1985-2002 by the U.S. Geological Survey National Water-Quality Assessment (NAWQA) Program. THMs are a group of volatile organic compounds (VOCs) with natural and anthropogenic sources that are of interest because they are associated with acute and chronic health problems in humans. THMs occur in water primarily from chlorination and are classified as disinfection by-products. In this report, the four THMs are discussed in the order of chloroform, bromodichloromethane, dibromochloromethane, and then bromoform; this sequence corresponds to largest to smallest chlorine content and smallest to largest bromine content. Four trihalomethanes were detected in less than 20 percent of samples from studies of (1) aquifers, (2) shallow ground water in agricultural areas, (3) shallow ground water in urban areas, (4) domestic wells, and (5) public wells. Detection frequencies for individual THMs in the five studies ranged from zero for shallow ground water in agricultural areas to 19.5 percent for shallow ground water in urban areas. None of the samples from aquifer studies, domestic wells, or public wells had total THM concentrations (the sum of the concentrations of chloroform, bromodichloromethane, dibromochloromethane, and bromoform) greater than or equal to the U.S. Environmental Protection Agency Maximum Contaminant Level of 80 micrograms per liter (?g/L). Comparisons of results among studies of aquifers, shallow ground water in agricultural areas, and shallow ground water in urban areas were used to describe the occurrence of the four THMs in ground water for three different land-use settings-mixed, agricultural, and urban, respectively. At the 0.2-?g/L assessment level, one or more of the four THMs were detected in 7.9 percent of the samples from aquifer studies, 2.2 percent of the samples from shallow ground water in agricultural areas, and 19.5 percent of the samples from shallow ground water in urban areas. In general, detection frequencies and concentrations of the four THMs were greater in shallow ground water in urban areas compared to aquifer studies and to shallow ground water in agricultural areas. For all three of these studies, the most common two-THM mixture at the 0.2-?g/L assessment level was chloroform-bromodichloromethane, and this was the only two-THM mixture found in samples of shallow ground water in agricultural areas. Comparisons of results between studies of domestic wells and public wells were used to describe the occurrence of the four THMs in two different supplies of ground water used for drinking water. At the 0.2-?g/L assessment level, one or more of the four THMs were detected in 5.2 percent of the domestic well samples and in 14.7 percent of the public well samples. In general, detection frequencies and THM concentrations were greater in samples from public wells than from domestic wells. At the 0.2-?g/L assessment level, the six possible two-THM mixtures occurred about six times more frequently in samples from public wells than from domestic wells. One of the most common two-THM mixtures in samples from domestic and public wells was bromodichloromethane-dibromochloromethane. Detection frequency is associated with the chlorine content of the THM compound. In general, for each of the five studies, as the chlorine content of the THM compound decreased, the detection frequency at the 0.2-?g/L assessment level also decreased. The exception was the study of public wells in which the detection frequency of the THMs decreased in the following order: chloroform, bromoform, dibromochloromethane, and bromodichloromethane. At the 0.2-?g/L assessment level, the median concentration for one or more of the four THMs ranged from 0.3 ?g/L (shallow ground water in agricultural a

Scientific Investigations Report

The global proliferation of aquatic, benthic Microcoleus : Taxonomy, distribution, toxin production, ecology, and future directions

There have been sporadic reports of aquatic, benthic Microcoleus proliferations in freshwater rivers, lakes, and reservoirs for four decades, with reports increasing in frequency over the last twenty years, suggesting a possible rise in their global distribution, frequency, and intensity. Microcoleus can produce anatoxins which are neurotoxic, and ingestion of toxic mats has caused hundreds of dog fatalities and raised serious human and ecological health concerns. This review synthesizes and evaluates current knowledge on Microcoleus distribution, taxonomy, toxin production, toxicity, ecology, environmental drivers, and biotic interactions. Toxin-producing Microcoleus have been reported in at least 18 countries, though many regions have not conducted toxin testing, suggesting a broader but under-reported distribution. Proliferations occur across diverse habitats, including cobble-bedded streams, large sandy rivers, reservoirs, and lakes. Microcoleus proliferations also occur on macrophytes, both in lakes and rivers. Genomic analyses currently classify anatoxin-producing Microcoleus into distinct species, with all known anatoxin-producers isolated from freshwater ecosystems. Anatoxin concentrations vary widely over space and time, within and among waterbodies. While studies on environmental drivers remain limited, research in cobble-bedded rivers suggests that moderate enrichment of dissolved inorganic nitrogen and low dissolved reactive phosphorus concentrations in the water column promote proliferation. Metagenomic approaches have revealed unique nutrient acquisition and storage strategies used by Microcoleus . Key knowledge gaps remain around the environmental and ecological triggers of proliferation, toxin production, genomic diversity and microbial interactions. Addressing these gaps through coordinated, global studies using robust datasets and consistent methods is critical to improve prediction, monitoring, and mitigation of this increasingly widespread public and ecological health threat.

Water Research

Decision-making for managing harmful algal blooms

Cyanobacteria are a global water-quality concern because these organisms can develop into harmful blooms that affect ecologic, economic, and public health. U.S. Geological Survey scientists worked with the New York State Office of Parks, Recreation and Historic Preservation and the New York State Department of Environmental Conservation to develop a structured decision-making template for managing cyanobacterial harmful algal blooms. This approach can help natural resource managers make decisions to manage water bodies threatened by harmful algal blooms.

New York