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Biocrust science and global change

Global environmental changes such as climate and land‐use change affect ecosystems worldwide, and this New Phytologist Virtual Issue brings together fundamental research questions and novel approaches associated with the study of biological soil crusts in the context of such shifts. In a changing world, organisms can display a limited set of responses that will determine their persistence over varied spatial and temporal scales. Specifically, organisms might tolerate the change – for example, via phenotypic plasticity – and remain present in local communities. Alternatively, organisms might shift or retract their range to match their historical niche, they may adapt to the directional selection pressures imposed by change, or they could be driven to local (and possibly global) extinction. Efforts to understand which of these responses particular plant species or assemblages will exhibit are necessary for predicting changes in ecosystem functioning and trophic interactions under global change scenarios, and for managing and supporting sustainable terrestrial ecosystems. Accordingly, the assessment of plant responses to global change has become a significant research focus. Despite this impressive effort, our understanding and combined work to measure the responses to global change for species and communities of nonvascular autotrophs, such as the cyanobacteria, lichens, and bryophytes that form biological soil crusts (Fig. 1), remain rare compared with the large focus on vascular plants (Fig. 2; Reed et al., 2016). Nevertheless, these nonvascular photosynthetic communities and their responses to change could have critical implications for determining ecosystem structure and function at the global‐scale (Elbert et al., 2012; Ferrenberg et al., 2017; Rodriguez‐Caballero et al., 2018).

New Phytologist↗

Thermoluminescence and new 14C age estimates for late quaternary loesses in southwestern Nebraska

Loess of late Quaternary age mantles most of Nebraska south of the Platte River Valley. At least five late Quaternary loesses are recognized: from oldest to youngest, one or more undifferentiated pre-Illinoian loesses, the Loveland Loess, the Gilman Canyon Loess, which exhibits a well developed soil and rests unconformably on the Sangamon soil, the Peoria Loess capped by the Brady soil, and the Bignell Loess, which is distributed discontinuously. Previous research shows that the Loveland Loess is Illinoian, the Gilman Canyon Loess and Peoria Loess are Wisconsin, and the Bignell Loess is Holocene. We present here the first thermoluminescence (TL) age estimates and new 14 C ages for these late Quaternary loesses at two key sections in southwestern Nebraska, the Eustis ash pit and the Bignell Hill road cut. TL age estimates from all samples collected from Eustis ash pit and Bignell Hill were internally consistent. TL and 14 C age estimates from these two sections generally agree and support previous age determinations. The TL age estimate on Loveland Loess indicates deposition at 163 ka. TL and radiocarbon age estimates indicate that Gilman Canyon Loess, believed to be deposited during the Farmdale interstade, first began to accumulate at about 40 ka: the lower part of the Gilman Canyon Loess is 36 ka at Eustis and the middle of the unit is 30 ka at Bignell Hill. The lower and upper parts of the Peoria Loess give age estimates of 24 ka and 17 ka, respectively. TL age estimates for deposition of the Bignell Loess are 9 ka near the base, in agreement with radiocarbon age estimates, and 6 ka immediately below the modern soil, substantiating its Holocene age. Comparisons of TL age estimates with δ 18 O and insolation curves which show loess deposition during interglacial and interstadial as well as glacial periods, indicate that loess deposition on the Great Plains can occur under a variety of climatic conditions.

Geomorphology↗

The presence of antibiotic resistance genes in coastal soil and sediment samples from the eastern seaboard of the USA

Infections from antibiotic resistant microorganisms are considered to be one of the greatest global public health challenges that result in huge annual economic losses. While genes that impart resistance to antibiotics (AbR) existed long before the discovery and use of antibiotics, anthropogenic uses of antibiotics in agriculture, domesticated animals, and humans are known to influence the prevalence of these genes in pathogenic microorganisms. It is critical to understand the role that natural and anthropogenic processes have on the occurrence and distribution of antibiotic resistance in microbial populations to minimize health risks associated with exposures. As part of this research, 15 antibiotic resistance genes were analyzed in coastal sediments and soils along the eastern seaboard of the USA using presence/absence quantitative and digital polymerase chain reaction assays. Samples (53 soil and 192 sediment samples including 54 replicates) were collected from a variety of coastal settings where human and wildlife exposure is likely. At least one of the antibiotic resistance genes was detected in 76.4% of the samples. Samples that contained at least five or more antibiotic resistance genes (5.7%) where typically hydrologically down gradient of watersheds influenced by combined sewer outfalls (CSO). The most frequently detected antibiotic resistance target genes were found in 33.2%, 34.4%, and 42.2% of samples (target genes bla SHV , tetO , and aadA2 , respectively). These data provide unique insight into potential exposure of AbR genes over a large geographical region of the eastern seaboard of the USA.

Connecticut, Delaware, District of Columbia, Maine↗

Vapor-phase exchange of perchloroethene between soil and plants

Tree core concentrations of tetrachloroethylene (perchloroethene, PCE) at the Riverfront Superfund Site in New Haven, MO, were found to mimic the profile of soil phase concentrations. The observed soil-tree core relationship was stronger than that of groundwater PCE to tree core concentrations at the same site. Earlier research has shown a direct, linear relationship between tree core and groundwater concentrations of chlorinated solvents and other organics. Laboratory-scale experiments were performed to elucidate this phenomenon, including determining partitioning coefficients of PCE between plant tissues and air and between plant tissues and water, measured to be 8.1 and 49 L/kg, respectively. The direct relationship of soil to tree core PCE concentrations was hypothesized to be caused by diffusion between tree roots and the soil vapor phase in the subsurface. The central findings of this research are discovering the importance of subsurface vapor-phase transfer for VOCs and uncovering a direct relationship between soil vapor-phase chlorinated solvents and uptake rates that impact contaminant translocation from the subsurface and transfer into the atmosphere. ?? 2005 American Chemical Society.

Environmental Science & Technology↗

Occurrence and temporal variability of methyl tert -butyl ether (MTBE) and other volatile organic compounds in select sources of drinking water : results of the focused survey

The large-scale use of the gasoline oxygenate methyl tert -butyl ether (MTBE), and its high solubility, low soil adsorption, and low biodegradability, has resulted in its detection in ground water and surface water in many places throughout the United States. Studies by numerous researchers, as well as many State and local environmental agencies, have discovered high levels of MTBE in soils and ground water at leaking underground gasoline-storage-tank sites and frequent occurrence of low to intermediate levels of MTBE in reservoirs used for both public water supply and recreational boating. In response to these findings, the American Water Works Association Research Foundation sponsored an investigation of MTBE and other volatile organic compounds (VOCs) in the Nation's sources of drinking water. The goal of the investigation was to provide additional information on the frequency of occurrence, concentration, and temporal variability of MTBE and other VOCs in source water used by community water systems (CWSs). The investigation was completed in two stages: (1) reviews of available literature and (2) the collection of new data. Two surveys were associated with the collection of new data. The first, termed the Random Survey, employed a statistically stratified design for sampling source water from 954 randomly selected CWSs. The second, which is the focus of this report, is termed the Focused Survey, which included samples collected from 134 CWS source waters, including ground water, reservoirs, lakes, rivers, and streams, that were suspected or known to contain MTBE. The general intent of the Focused Survey was to compare results with the Random Survey and provide an improved understanding of the occurrence, concentration, temporal variability, and anthropogenic factors associated with frequently detected VOCs. Each sample collected was analyzed for 66 VOCs, including MTBE and three other ether gasoline oxygenates (hereafter termed gasoline oxygenates). As part of the Focused Survey, 451 source-water samples and 744 field quality-control (QC) samples were collected from 78 ground-water, 39 reservoir and (or) lake, and 17 river and (or) stream source waters at fixed intervals for a period of 1 year. Using a common assessment level of 0.2 μg/L (micrograms per liter) (2.0 μg/L for methyl ethyl ketone), 37 of the 66 VOCs analyzed were detected in both surveys. However, VOCs, especially MTBE and other gasoline oxygenates, were detected more frequently in the Focused Survey than in the Random Survey. MTBE was detected in 55.5 percent of the CWSs sampled in the Focused Survey and in 8.7 percent of those sampled in the Random Survey. Little difference in occurrence, however, was observed for trihalomethanes (THMs), which were detected in 16.4 and 14.8 percent of Focused Survey and Random Survey CWSs, respectively. This may indicate a pervasive occurrence of THMs in several source-water types, regardless of CWS size or geographic location. Using data at or above the method detection limit to assess temporal variability and anthropogenic factors associated with frequent detection of select VOCs, concentrations in the Focused Survey in ground-water, reservoir, and river source waters were typically less than 1 μg/L. Also, at a 95-percent confidence interval, no statistically significant differences were observed in comparing concentrations in the first and second ground-water sample. A weak seasonal pattern was observed in samples collected from reservoirs and lakes where gasoline oxygenates and other gasoline compounds were detected more frequently during spring and summer, presumedly a result of increased use of motorized watercraft during these seasons. In contrast, seasonal patterns were not observed in samples collected from rivers and streams. The lack of seasonal differences in river and stream source waters sampled may indicate a common and continuous source of contamination. The most frequently detected VOC (MTBE) and the two most frequently occurring subgroups of VOCs (gasoline oxygenates and THMs) detected in CWS source waters were further characterized to evaluate some anthropogenic factors that may better explain their frequent occurrence. Gasoline oxygenates were detected in 73.9 percent of all CWSs sampled. The concentration of gasoline oxygenates was slightly correlated with watercraft use on reservoirs inside MTBE high-use areas (r2=0.3783) but not outside of these areas (r2=0.0242). In general, the concentration of gasoline oxygenates increased as watercraft use increased. THMs were detected in 47.8 percent of the CWSs supplied by surface water. The frequent occurrence of THMs in reservoir source waters was determined to be an artifact of disinfection and the recycling of chlorinated water to these reservoirs. All CWSs with frequent occurrence of THMs served by a reservoir indicated that chlorine was added to waters for various reasons and that the chlorinated water was then released back to, or upstream of, the reservoir or lake that was sampled.

Water-Resources Investigations Report↗

Biocrusts indicators of livestock grazing effects on soil stability in sagebrush steppe: A case study from a long-term experiment in the northern Great Basin

Biocrusts are sensitive to changes in livestock grazing intensity in arid rangelands and may be useful indicators of ecosystem functions, particularly soil properties like soil stability, which may suggest the potential for soil erosion. We compared biocrust community composition and surface soil stability in a big sagebrush (Artemisia tridentata) steppe rangeland in the northwestern Great Basin in several paired sites, with or without long-term cattle grazing exclusion, and similar soils (mostly sandy loams), climate, and vegetation composition. We found that livestock grazing was associated with both lower surface soil stability and cover of several biocrust morphogroups, especially lichens, compared with sites with long-term livestock exclusion. Surface soil stability did not modify the effects of grazing on most biocrust components via interactive effects. Livestock grazing effects on total biocrust cover were partially mediated by changes in surface soil stability. Though lichens were more sensitive to grazing disturbance, our results suggest that moss (mostly Tortula ruralis in this site) might be a more readily observable indicator of grazing-related soil stability change in this area due to their relatively higher abundance compared with lichens (moss: mean, 8.5% cover, maximum, 96.1%, lichens: mean, 1.0% cover, maximum, 14.1%). These results highlight the potential for biocrust components as sensitive indicators of change in soil-related ecosystem functions in sagebrush steppe rangelands. However, further research is needed to identify relevant indicator groups across the wide range of biocrust community composition associated with site environmental characteristics, variable grazing systems, other rangeland health metrics, and other disturbance types such as wildfire.

Northern Great Basin↗

Ground water and climate change

As the world's largest distributed store of fresh water, ground water plays a central part in sustaining ecosystems and enabling human adaptation to climate variability and change. The strategic importance of ground water for global water and food security will probably intensify under climate change as more frequent and intense climate extremes (droughts and floods) increase variability in precipitation, soil moisture and surface water. Here we critically review recent research assessing the impacts of climate on ground water through natural and human-induced processes as well as through groundwater-driven feedbacks on the climate system. Furthermore, we examine the possible opportunities and challenges of using and sustaining groundwater resources in climate adaptation strategies, and highlight the lack of groundwater observations, which, at present, limits our understanding of the dynamic relationship between ground water and climate.

Nature Climate Change↗

Panola Mountain, Georgia: A Water, Energy, and Biogeochemical Budgets Program Site

The Panola Mountain Research Watershed (PMRW) is a 41-hectare forested watershed in the southern Piedmont physiographic province near Atlanta, Georgia. The watershed contains a naturally regenerated second-growth forest on abandoned agricultural land, typical of the Piedmont. Research at PMRW has focused on how streamflow is generated, and in particular, on how water and solutes move from hillslopes to the stream. The research shows that streamflow and solute concentrations of precipitation, throughfall, soil water and streamwater change rapidly during rainstorms. Furthermore, these variations are strongly associated with the soil-moisture conditions, the soil type and thickness, and the rainfall intensity, all of which effect the chemical interactions and mixing of water traveling along hydrological pathways over and through the bedrock and soils.

Georgia↗

Atmospheric deposition: Sample handling, storage, and analytical procedures for chemical characterization of event-based samples in North Dakota

The North Dakota atmospheric-deposition network uses HASL-type collectors to sample wet and dry deposition on an event basis for analysis of 8 principal mineral, 3 nutrient, and 15 trace constituents. Comparison of the compositions of wet deposition collected in adjacent collectors sampled on weekly and event bases indicates that weekly sampling leads to significant contamination of wet deposition samples with dry deposition. Elevation of the collectors 10 feet above ground surface may decrease wind-blown soil contamination significantly. This manual presents a summary of the research procedures adopted by the North Dakota District of the U.S. Geological Survey to investigate the consequences of increased fossil-fuel combustion on the quality of atmospheric deposition. The manual has been tested by field operatives for 3 years and shown to be an effective field guide and management tool.

North Dakota↗

Simulated response of the High Plains aquifer to ground-water withdrawals in the Upper Republican Natural Resources District, Nebraska

The U.S. Geological Survey, in cooperation with the National Soil Tilth Laboratory of the U.S. Department of Agriculture, Agricultural Research Service, conducted a study as part of the multi- scale, interagency Management Systems Evaluation Area (MSEA) program to evaluate the effects of agricultural management (farming) systems on water quality. Data on surface flow, tileflow, and streamflow in the Walnut Creek watershed just south of Ames, Iowa, were collected during April 1991-September 1993 at five sites with drainage areas ranging from 366 to 5,130 hectares. Precipitation, flow discharge, and concentration, loads, and yields of nitrate as nitrogen, atrazine, and metolachlor were analyzed to relate the transport of agricultural chemicals to major water-flow processes and to examine and transport differences among three subwatersheds. Antecedent conditions and basin-characteristic differences had significant effects on the flow response from the subwatersheds. Monthly streamflow-to- precipitation ratios were greater than 1.0, as a result of snowmelt, and negative when streamflow was lost to the ground-water system in the downstream subwatershed. Dry antecedent conditions resulted in ratios less than 0.3 (July 1992), whereas wet antecedent conditions resulted in ratios from 0.7 to almost 1.0 (July 1993) during months with similar large rainfall amounts. Most of the streamflow from the upland subwatersheds came from tileflow. Surface flow (surface runoff, interflow, and return flow0 was highly variable and intermittent, usually lasting for only a few days after a storm, although it could be the dominant source of flow when stormflow was large. Tileflow was less variable and much more persistent, ceasing only after prolonged dry periods. Large quantities of nitrate as nitrogen were transported in Walnut Creek, with concen- trations often greater than the Maximum Contaminant Level of 10 milligrams per liter established by the U.S. Environmental Protection Agency for finished drinking water. In the upland subwatersheds, ground-water flow from the tiles appears to have been the primary means of transport to the streams. Concentrations in tileflow and streamflow generally were 4 to 16 milligrams per liter, with the lower concen- trations often the result of dilution by surface runoff. Loss ratios, chemical yields expressed as a percentage of average application rates of nitrate as nitrogen for October 1992-September 1993, were about 10 percent for surface flow and more than 100 percent for tileflow from the 366-hectare basin and were more than 200 percent for streamflow from the downstream subwatershed. Concentrations of atrazine and metolachlor in streamflow, typically, were less than the Maximum Contaminant Level of 3.0 micrograms per liter, but were as high as 59 and 80 micrograms per liter, respectively, during stormflow. Concentrations as high as 170 micrograms per liter occurred in tileflow, but these were related to surface flow through surface inlets. The transport of herbicides was extremely variable, with most of the loads occurring during stormflow. Atrazine appeared more susceptable to transport losses to streamflow than did metolachlor. Loss ratios for streamflow from the subwatersheds for April- September periods were 0.3 to 20 percent for atrazine and 0.1 to 2.9 percent for metolachlor. Chemical loss ratios indicated differences in the transport characteristics of the three subwatersheds. The downstream subwatershed, which has steeper terrain, a more-developed natural drainage system, and fewer tiles than the two upland subwatersheds, had the largest loss rates for all three chemicals--206 percent for nitrate as nitrogen (October 1992-September 1993) and 20 percent for atrazine and 2.9 percent for metolachlor (April-September 1993). For May-July 1993, when most of the herbicides were transported, the downstream subwatershed also had the largest cumulative unit discharge and the largest streamflow-to-precipitation ra

Water-Resources Investigations Report↗

Transport of agricultural chemicals in surface flow, tileflow, and streamflow of Walnut Creek Watershed near Ames, Iowa, April 1991-September 1993

The U.S. Geological Survey, in cooperation with the National Soil Tilth Laboratory of the U.S. Department of Agriculture, Agricultural Research Service, conducted a study as part of the multi-scale, inter-agency Management Systems Evaluation Area (MSEA) program to evaluate the effects of agricultural management (farming) systems on water quality. Data on surface flow, tileflow, and streamflow in the Walnut Creek watershed just south of Ames, Iowa, were collected during April 1991-September 1993 at five sites with drainage areas ranging from 366 to 5,130 hectares. Precipitation, flow discharge, and concentration, loads, and yields of nitrate as nitrogen, atrazine, and metolachlor were analyzed to relate the transport of agricultural chemicals to major water-flow processes and to examine flow and transport differences among three subwatersheds. Antecedent conditions and basin-characteristic differences had significant effects on the flow response from the subwatersheds. Monthly streamflow-to-precipitation ratios were greater than 1.0, as a result of snowmelt, and negative when streamflow was lost to the ground-water system in the downstream subwatershed. Dry antecedent conditions resulted in ratios less than 0.3 (July 1992), whereas wet antecedent conditions resulted in ratios from 0.7 to almost 1.0 (July 1993) during months with similar large rainfall amounts. Most of the streamflow from the upland subwatersheds came from tileflow. Surface flow (surface runoff, interflow, and return flow) was highly variable and intermittent, usually lasting for only a few days after a storm, although it could be the dominant source of flow when stormflow was large. Tileflow was less variable and much more persistent, ceasing only after prolonged dry periods. Large quantities of nitrate as nitrogen were transported in Walnut Creek, with concentrations often greater than the Maximum Contaminant Level of 10 milligrams per liter established by the U.S. Environmental Protection Agency for finished drinking water. In the upland subwatersheds, ground-water flow from the tiles appears to have been the primary means of transport to the streams. Concentrations in tileflow and streamflow generally were 4 to 16 milligrams per liter, with the lower concentrations often the result of dilution by surface runoff. Loss ratios, chemical yields expressed as a percentage of average application rates of nitrate as nitrogen for October 1992-September 1993, were about 10 percent for surface flow and more than 100 percent for tileflow from the 366-hectare basin and were more than 200 percent for streamflow from the downstream subwatershed. Concentrations of atrazine and metolachlor in streamflow, typically, were less than the Maximum Contaminant Level of 3.0 micrograms per liter, but were as high as 59 and 80 micrograms per liter, respectively, during stormflow. Concentrations as high as 170 micrograms per liter occurred in tileflow, but these were related to surface flow through surface inlets. The transport of herbicides was extremely variable, with most of the loads occurring during stormflow. Atrazine appeared more susceptible to transport losses to streamflow than did metolachlor. Loss ratios for streamflow from the subwatersheds for April-September periods were 0.3 to 20 percent for atrazine and 0.1 to 2.9 percent for metolachlor. Chemical loss ratios indicated differences in the transport characteristics of the three subwatersheds. The downstream subwatershed, which has steeper terrain, a more-developed natural drainage system, and fewer tiles than the two upland subwatersheds, had the largest loss rates for all three chemicals 206 percent for nitrate as nitrogen (October 1992-September 1993) and 20 percent for atrazine and 2.9 percent for metolachlor (April-September 1993). For May-July 1993, when most of the herbicides were transported, the downstream subwatershed also had the largest cumulative unit discharge and the largest streamflow-to-precipitation ratios.

Iowa↗

Moist-soil seed abundance in managed wetlands in the Mississippi Alluvial Valley

Managed moist-soil units support early succession herbaceous vegetation that produces seeds, tubers, and other plant parts used by waterfowl in the Mississippi Alluvial Valley (MAV), USA. We conducted a stratified multi-stage sample survey on state and federal lands in the MAV of Arkansas, Louisiana, Mississippi, and Missouri during autumns 2002?2004 to generate a contemporary estimate of combined dry mass of seeds and tubers (herein seed abundance) in managed moist-soil units for use by the Lower Mississippi Valley Joint Venture (LMVJV) of the North American Waterfowl Management Plan. We also examined variation in mean seed abundance among moist-soil units in 2003 and 2004 in relation to management intensity (active or passive), soil pH and nutrient levels, proportional occurrence of plant life-forms (e.g., grass, flatsedge, and forb; vine; woody plants), and unit area. Estimates of mean seed abundance were similar in 2002 (X over bar = 537.1 kg/ha, SE = 100.1) and 2004 (X over bar = 555.2 kg/ha, SE = 105.2) but 35?40% less in 2003 (X over bar = 396.8 kg/ha, SE = 116.1). Averaged over years, seed abundance was 496.3 kg/ha (SE = 62.0; CV = 12.5%). Multiple regression analysis indicated seed abundance varied among moist-soil units inversely with proportional occurrence of woody vegetation and unit area and was greater in actively than passively managed units (R2adj = 0.37). Species of early succession grasses occurred more frequently in actively than passively managed units (P < 0.09), whereas mid- and late-succession plants occurred more often in passively managed units (P < 0.02). We recommend the LMVJV consider 556 kg/ha as a measure of seed abundance for use in estimating carrying capacity in managed moist-soil units on public lands in the MAV. We recommend active management of moist-soil units to achieve maximum potential seed production and further research to determine recovery rates of seeds of various sizes from core samples and the relationship between seed abundance and unit area.

Journal of Wildlife Management↗

Synthesis of thirty years of surface water quality and aquatic biota data in Shenandoah National Park: Collaboration between the US Geological Survey and the National Park Service

The eastern United States has been the recipient of acidic atmospheric deposition (hereinafter, “acid rain”) for many decades. Deleterious effects of acid rain on natural resources have been well documented for surface water (e.g., Likens et al. 1996; Stoddard et al. 2001), soils (Bailey et al. 2005), forest health (Long et al. 2009), and habitat suitability for stream biota (Baker et al. 1993). Shenandoah National Park (SNP) is located in northern and central Virginia and consists of a long, narrow strip of land straddling the Blue Ridge Mountains (Figure 1). The park’s elevated topography and location downwind of the Ohio River valley, where many acidic emissions to the atmosphere are generated (NSTC 2005), have made it a target for acid rain. Characterizing the link between air quality and water quality as related to acid rain, contaminants, soil conditions, and forest health is a high priority for research and monitoring in SNP. The US Geological Survey (USGS) and SNP have had a long history of collaboration on documenting acid rain effects on the park’s natural resources, starting in 1985 and continuing to the present (Lynch and Dise 1985; Rice et al. 2001, 2004, 2005, 2007; Deviney et al. 2006, 2012; Jastram et al. 2013).

Virginia↗

Assessing wildlife benefits and carbon storage from restored and natural coastal marshes in the Nisqually River Delta: Determining marsh net ecosystem carbon balance

Working in partnership since 1996, the U.S. Fish and Wildlife Service and the Nisqually Indian Tribe have restored 902 acres of tidally influenced coastal marsh in the Nisqually River Delta (NRD), making it the largest estuary-restoration project in the Pacific Northwest to date. Marsh restoration increases the capacity of the estuary to support a diversity of wildlife species. Restoration also increases carbon (C) production of marsh plant communities that support food webs for wildlife and can help mitigate climate change through long-term C storage in marsh soils. In 2015, an interdisciplinary team of U.S. Geological Survey (USGS) researchers began to study the benefits of carbon for wetland wildlife and storage in the NRD. Our primary goals are (1) to identify the relative importance of the different carbon sources that support juvenile chinook ( Oncorhynchus tshawytscha ) food webs and contribute to current and historic peat formation, (2) to determine the net ecosystem carbon balance (NECB) in a reference marsh and a restoration marsh site, and (3) to model the sustainability of the reference and restoration marshes under projected sea-level rise conditions along with historical vegetation change. In this fact sheet, we focus on the main C sources and exchanges to determine NECB, including carbon dioxide (CO 2 ) uptake through plant photosynthesis, the loss of CO 2 through plant and soil respiration, emissions of methane (CH 4 ), and the lateral movement or leaching loss of C in tidal waters.

Washington↗

Ground and surface temperature variability for remote sensing of soil moisture in a heterogeneous landscape

At the Little River Watershed (LRW) heterogeneous landscape near Tifton Georgia US an in situ network of stations operated by the US Department of Agriculture-Agriculture Research Service-Southeast Watershed Research Lab (USDA-ARS-SEWRL) was established in 2003 for the long term study of climatic and soil biophysical processes. To develop an accurate interpolation of the in situ readings that can be used to produce distributed representations of soil moisture (SM) and energy balances at the landscape scale for remote sensing studies, we studied (1) the temporal and spatial variations of ground temperature (GT) and infra red temperature (IRT) within 30 by 30 m plots around selected network stations; (2) the relationship between the readings from the eight 30 by 30 m plots and the point reading of the network stations for the variables SM, GT and IRT; and (3) the spatial and temporal variation of GT and IRT within agriculture landuses: grass, orchard, peanuts, cotton and bare soil in the surrounding landscape. The results showed high correlations between the station readings and the adjacent 30 by 30 m plot average value for SM; high seasonal independent variation in the GT and IRT behavior among the eight 30 by 30 m plots; and site specific, in-field homogeneity in each 30 by 30 m plot. We found statistical differences in the GT and IRT between the different landuses as well as high correlations between GT and IRT regardless of the landuse. Greater standard deviations for IRT than for GT (in the range of 2-4) were found within the 30 by 30 m, suggesting that when a single point reading for this variable is selected for the validation of either remote sensing data or water-energy models, errors may occur. The results confirmed that in this landscape homogeneous 30 by 30 m plots can be used as landscape spatial units for soil moisture and ground temperature studies. Under this landscape conditions small plots can account for local expressions of environmental processes, decreasing the errors and uncertainties in remote sensing estimates caused by landscape heterogeneity.

Journal of Hydrology↗

The influence of precipitation, vegetation and soil properties on the ecohydrology of sagebrush steppe rangelands on the INL site

The INL Site and other landscapes having sagebrush steppe vegetation are experiencing a simultaneous change in climate and floristics that result from increases in exotic species. Determining the separate and combined/interactive effects of climate and vegetation change is important for assessing future changes on the landscape and for hydrologic processes. This research uses the 72 experimental plots established and initially maintained for many years as the “Protective Cap Biobarrier Experiment” by Dr. Jay Anderson and the Stoller ESER program, and the experiment is also now referred to as the “INL Site Ecohydrology Study.” We are evaluating long-term impacts of different plant communities commonly found throughout Idaho subject to different precipitation regimes and to different soil depths. Treatments of amount and timing of precipitation (irrigation), soil depth, and either native/perennial or exotic grass vegetation allow researchers to investigate how vegetation, precipitation and soil interact to influence soil hydrology and ecosystem biogeochemistry. This information will be used to improve a variety of models, as well as provide data for these models.

Report↗

Inferring local competition intensity from patch size distributions: a test using biological soil crusts

Dryland vegetation is inherently patchy. This patchiness goes on to impact ecology, hydrology, and biogeochemistry. Recently, researchers have proposed that dryland vegetation patch sizes follow a power law which is due to local plant facilitation. It is unknown what patch size distribution prevails when competition predominates over facilitation, or if such a pattern could be used to detect competition. We investigated this question in an alternative vegetation type, mosses and lichens of biological soil crusts, which exhibit a smaller scale patch-interpatch configuration. This micro-vegetation is characterized by competition for space. We proposed that multiplicative effects of genetics, environment and competition should result in a log-normal patch size distribution. When testing the prevalence of log-normal versus power law patch size distributions, we found that the log-normal was the better distribution in 53% of cases and a reasonable fit in 83%. In contrast, the power law was better in 39% of cases, and in 8% of instances both distributions fit equally well. We further hypothesized that the log-normal distribution parameters would be predictably influenced by competition strength. There was qualitative agreement between one of the distribution's parameters (&mu;) and a novel intransitive (lacking a 'best' competitor) competition index, suggesting that as intransitivity increases, patch sizes decrease. The correlation of &mu; with other competition indicators based on spatial segregation of species (the C-score) depended on aridity. In less arid sites, &mu; was negatively correlated with the C-score (suggesting smaller patches under stronger competition), while positive correlations (suggesting larger patches under stronger competition) were observed at more arid sites. We propose that this is due to an increasing prevalence of competition transitivity as aridity increases. These findings broaden the emerging theory surrounding dryland patch size distributions and, with refinement, may help us infer cryptic ecological processes from easily observed spatial patterns in the field.

Oikos↗

Facilitating comparable research in seedling functional ecology

Ecologists have worked to ascribe function to the variation found in plant populations, communities and ecosystems across environments for at least the past century. The vast body of research in functional ecology has drastically improved understanding of how individuals respond to their environment, communities are assembled and ecosystems function. However, with limited exceptions, few studies have quantified differences in plant function during the earliest stages of the plant life cycle, and fewer have tested how this early variability shapes populations, communities and ecosystems. Drawing from the literature and our collective experience, we describe the current state of knowledge in seedling functional ecology and provide examples of how this subdiscipline can enrich our fundamental understanding of plant function across levels of organisation. To inspire progressive work in this area, we also outline key considerations involved in seedling functional research (who, what, when, where and how to measure seedling traits) and identify remaining challenges and gaps in understanding around methodological approaches. Within this conceptual synthesis, we highlight three critical areas in seedling ecology for future research to target. First, given wide variation in the definition of a ‘seedling’, we provide a standard definition based on seed reserve dependence while emphasising the need to measure ontogenetic variation more clearly both within and following the seedling stage. Second, studies demonstrate that seedlings can be studied in multiple media (e.g. soil, agar, filter paper) and conditions (e.g. field, greenhouse, laboratory). We recommend that researchers select methods based on explicit goals, yet follow standard guidelines to reduce methodological noise across studies. Third, research is critically needed to assess the implications of different methodologies on trait measurement and compatibility across studies. By highlighting the importance of seedling functional ecology and suggesting pathways to address key challenges, we aim to inspire future research that generates useful and comparable data on seedling functional ecology. This work is critical to explain variation within and among populations, communities and ecosystems and integrate this most vulnerable stage of plant life into ecological frameworks.

Methods in Ecology & Evolution↗