Validation of daily age in Grass Carp reared in tanks and outdoor ponds in the U.S. Midwest

Abstract

Objective Accurate and precise age estimation is essential for understanding early life history, recruitment, and population dynamics in fish. However, daily otolith increment deposition has not been validated for Grass Carp Ctenopharyngodon idella in the Midwestern United States. Our objective was to validate daily increment deposition in lapilli otoliths of known-age Grass Carp that were reared under controlled laboratory and outdoor pond environments and to evaluate the reliability of otolith-based daily age estimation across early life stages. Methods Larval Grass Carp that were hatched from captive broodstock were reared in indoor tanks and outdoor ponds and sampled between 6 and 120 d posthatch. The lapilli otoliths were extracted, lightly sanded, and independently read three times to estimate age. The estimated ages were compared with known chronological age to assess the accuracy and precision of daily increment counts across rearing environments. Results Daily increment deposition was validated through 51 d posthatch for the tank-reared fish and through 69 d posthatch for the pond-reared fish. Beyond these ages, the estimated ages increasingly underestimated known age due to compression of daily increments near the otolith core and outer margin, particularly in slower growing individuals. Growth rates differed substantially between the two rearing environments and influenced increment spacing and readability. Otolith size was strongly correlated with the total length of the fish but decreased relative to chronological age as increment compression increased, particularly in fish exceeding approximately 40–50 mm total length (TL) in tank-reared and 60–70 mm TL in pond-reared individuals. Conclusions Lapilli otoliths provide reliable daily age estimates for Grass Carp during early life stages under both laboratory and pond conditions. These validated methods enable robust estimation of hatch timing and early growth, providing a foundational tool for studying early life history and informing control efforts for invasive Grass Carp populations