Genetic structure of an expanding population of Humpback Chub in Grand Canyon

Abstract

Objective Humpback Chub (HBC) Gila cypha in Grand Canyon declined in abundance and distribution over the latter part of the 20th century but have substantially increased in abundance and distribution over the past two decades. Although previous genetic work suggested that HBC in Grand Canyon belong to one genetic group, here we evaluate the genetic structure of HBC in Grand Canyon to determine whether relic populations in western Grand Canyon may have contributed unique variation to the recent population expansion or whether differences in behavior linked to migration in eastern Grand Canyon may promote assortative mating and heretofore unrecognized population structure. Methods Using fin clips collected from 167 individual HBC representing four sampling sites within Grand Canyon, we extracted DNA and developed data sets consisting of approximately 20,000 anonymous genomic loci. We quantified patterns of genetic diversity, and we accounted for outlier single-nucleotide polymorphisms to ensure that our interpretations of genetic patterns were not misled by adaptive processes and did not mask adaptively important genetic variation. Results Despite behavioral variation and the possibility that individuals with unique genetic variation survived in isolated warmwater sites within western Grand Canyon, HBC were not differentiated by sampling site or by differences in migratory behavior. Heterozygosity and nucleotide diversity were consistently high among sampling sites, and inbreeding coefficients were close to zero. Conclusions The HBC in Grand Canyon constitute a single genetic population. Our results do not preclude a genetic basis to migratory behavior, but our data suggest that this trait does not lead to assortative mating. Furthermore, while HBC may have survived in discontiguous warmwater refugia in western Grand Canyon during decades when the main stem was too cold for spawning, our data did not reveal any noticeable spatial variability in HBC genetics in the main stem after the recent HBC population expansion.