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Acetylene-fueled trichloroethene reductive dechlorination in a groundwater enrichment culture

Sara Gushgari-Doyle·Ronald S. Oremland·Ray Keren·Shaun Baesman·Denise M. Akob·Jillian F. Banfield·Lisa Alvarez-Cohen
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Abstract

In aquifers, acetylene (C 2 H 2 ) is a product of abiotic degradation of trichloroethene (TCE) catalyzed by in situ minerals. C 2 H 2 can, in turn, inhibit multiple microbial processes including TCE dechlorination and metabolisms that commonly support dechlorination, in addition to supporting the growth of acetylenotrophic microorganisms. Previously, C 2 H 2 was shown to support TCE reductive dechlorination in synthetic, laboratory-constructed cocultures containing the acetylenotroph Pelobacter sp. strain SFB93 and Dehalococcoides mccartyi strain 195 or strain BAV1. In this study, we demonstrate TCE and perchloroethene (PCE) reductive dechlorination by a microbial community enriched from contaminated groundwater and amended with C 2 H 2 as the sole electron donor and organic carbon source. The metagenome of the stable, enriched community was analyzed to elucidate putative community functions. A novel anaerobic acetylenotroph in the phylum Actinobacteria was identified using metagenomic analysis. These results demonstrate that the coupling of acetylenotrophy and reductive dechlorination can occur in the environment with native bacteria and broaden our understanding of biotransformation at contaminated sites containing both TCE and C 2 H 2 .

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Sara Gushgari-Doyle, Ronald S. Oremland, Ray Keren, Shaun Baesman, Denise M. Akob, Jillian F. Banfield, Lisa Alvarez-Cohen. 2021. Acetylene-fueled trichloroethene reductive dechlorination in a groundwater enrichment culture. https://doi.org/10.1128/mbio.02724-20

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