Concentration and retention of Toxoplasma gondii oocysts by marine snails demonstrate a novel mechanism for transmission of terrestrial zoonotic pathogens in coastal ecosystems

Colin Krusor; Woutrina A. Smith; M. Tim Tinker; Mary Silver; Patricia A. Conrad; Karen Shapiro

doi:10.1111/1462-2920.12927

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Concentration and retention of Toxoplasma gondii oocysts by marine snails demonstrate a novel mechanism for transmission of terrestrial zoonotic pathogens in coastal ecosystems

Colin Krusor·Woutrina A. Smith·M. Tim Tinker·Mary Silver·Patricia A. Conrad·Karen Shapiro

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Abstract

The parasite T oxoplasma gondii is an environmentally persistent pathogen that can cause fatal disease in humans, terrestrial warm-blooded animals and aquatic mammals. Although an association between T . gondii exposure and prey specialization on marine snails was identified in threatened California sea otters, the ability of kelp-dwelling snails to transmit terrestrially derived pathogens has not been previously investigated. The objective of this study was to measure concentration and retention of T . gondii by marine snails in laboratory aquaria, and to test for natural T . gondii contamination in field-collected snails. Following exposure to T . gondii -containing seawater, oocysts were detected by microscopy in snail faeces and tissues for 10 and 3 days respectively. Nested polymerase chain reaction was also applied as a method for confirming putative T . gondii oocysts detected in snail faeces and tissues by microscopy. T oxoplasma gondii was not detected in field-collected snails. Results suggest that turban snails are competent transport hosts for T . gondii . By concentrating oocysts in faecal pellets, snails may facilitate entry of T . gondii into the nearshore marine food web. This novel mechanism also represents a general pathway by which marine transmission of terrestrially derived microorganisms can be mediated via pathogen concentration and retention by benthic invertebrates.

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Colin Krusor, Woutrina A. Smith, M. Tim Tinker, Mary Silver, Patricia A. Conrad, Karen Shapiro. 2015-07-22. Concentration and retention of Toxoplasma gondii oocysts by marine snails demonstrate a novel mechanism for transmission of terrestrial zoonotic pathogens in coastal ecosystems. https://doi.org/10.1111/1462-2920.12927

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