Shifts in identity and activity of methanotrophs in arctic lake sediments in response to temperature changes

Abstract

Methane (CH 4 ) flux to the atmosphere is mitigated via microbial CH 4 oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH 4 emissions. We used DNA-based stable-isotope probing (SIP), quantitative PCR (Q-PCR), and pyrosequencing analyses to identify and characterize methanotrophic communities active at a range of temperatures (4°C, 10°C, and 21°C) in sediments (to a depth of 25 cm) sampled from Lake Qalluuraq on the North Slope of Alaska. CH 4 oxidation activity was measured in microcosm incubations containing sediments at all temperatures, with the highest CH 4 oxidation potential of 37.5 μmol g −1 day −1 in the uppermost (depth, 0 to 1 cm) sediment at 21°C after 2 to 5 days of incubation. Q-PCR of pmoA and of the 16S rRNA genes of type I and type II methanotrophs, and pyrosequencing of 16S rRNA genes in 13 C-labeled DNA obtained by SIP demonstrated that the type I methanotrophs Methylobacter , Methylomonas , and Methylosoma dominated carbon acquisition from CH 4 in the sediments. The identity and relative abundance of active methanotrophs differed with the incubation temperature. Methylotrophs were also abundant in the microbial community that derived carbon from CH 4 , especially in the deeper sediments (depth, 15 to 20 cm) at low temperatures (4°C and 10°C), and showed a good linear relationship ( R = 0.82) with the relative abundances of methanotrophs in pyrosequencing reads. This study describes for the first time how methanotrophic communities in arctic lake sediments respond to temperature variations.