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Protection of rainbow trout against infectious hematopoietic necrosis virus four days after specific or semi-specific DNA vaccination

S. E. LaPatra·S. Corbeil·G. R. Jones·W. D. Shewmaker·N. Lorenzen·Eric Anderson·Gael Kurath
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Abstract

A DNA vaccine against a fish rhabdovirus, infectious hematopoietic necrosis virus (IHNV), was shown to provide significant protection as soon as 4 d after intramuscular vaccination in 2 g rainbow trout ( Oncorhynchus mykiss ) held at 15°C. Nearly complete protection was also observed at later time points (7, 14, and 28 d) using a standardized waterborne challenge model. In a test of the specificity of this early protection, immunization of rainbow trout with a DNA vaccine against another fish rhabdovirus, viral hemorrhagic septicemia virus, provided a significant level of cross-protection against IHNV challenge for a transient period of time, whereas a rabies virus DNA vaccine was not protective. This indication of distinct early and late protective mechanisms was not dependent on DNA vaccine doses from 0.1 to 2.5 μg.

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S. E. LaPatra, S. Corbeil, G. R. Jones, W. D. Shewmaker, N. Lorenzen, Eric Anderson, Gael Kurath. 2001. Protection of rainbow trout against infectious hematopoietic necrosis virus four days after specific or semi-specific DNA vaccination. https://doi.org/10.1016/s0264-410x(01)00113-x

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