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USGS · 70006923

Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry

Steven T. K. Ha·Charles L. Wilkins·Sharon L. Abidi
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Abstract

A mixture of closely related streptomyces fermentation products, antimycin A, Is separated, and the components are identified by using reversed-phase high-performance liquid chromatography with directly linked 400-MHz proton nuclear magnetic resonance detection. Analyses of mixtures of three amino acids, alanine, glycine, and valine, are used to determine optimal measurement conditions. Sensitivity increases of as much as a factor of 3 are achieved, at the expense of some loss in chromatographic resolution, by use of an 80-μL NMR cell, Instead of a smaller 14-μL cell. Analysis of the antimycin A mixture, using the optimal analytical high performance liquid chromatography/nuclear magnetic resonance conditions, reveals it to consist of at least 10 closely related components.

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Steven T. K. Ha, Charles L. Wilkins, Sharon L. Abidi. 2002-05-01. Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry. https://doi.org/10.1021/ac00180a005

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