Hepatic microsomal N-oxidation and N-demethylation of N,N-dimethylaniline in red-winged blackbird compared with rat and other birds

H.P. Pan; J.R. Fouts; T.R. Devereux

doi:10.1016/0024-3205(75)90540-8

Home/Archive/USGS

USGS · 5224598

Hepatic microsomal N-oxidation and N-demethylation of N,N-dimethylaniline in red-winged blackbird compared with rat and other birds

H.P. Pan·J.R. Fouts·T.R. Devereux

View original source ↗Export metadata ↓

Abstract

Hepatic microsomes prepared from red-winged blackbirds and albino rats were incubated with N,N-dimethylaniline (DMA)_in complete incubation mixtures at pH 7.9 and 37?C for 10 min. Formaldehyde and N,N-dimethylaniline--oxide produced from DMA were measured. Redwings were found to have significantly lower N-demethylation activities than rats, and redwings had only marginal or no N-oxidation activities. Hepatic microsomes from redwings did not further metabolize the N-oxide. The N-oxidation and N-demethylation activities of brown-headed cowbirds, common grackles, and starlings were similar to those of redwings.

Explore related subjects

Life Sciences Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

H.P. Pan, J.R. Fouts, T.R. Devereux. 1975. Hepatic microsomal N-oxidation and N-demethylation of N,N-dimethylaniline in red-winged blackbird compared with rat and other birds. https://doi.org/10.1016/0024-3205(75)90540-8

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related USGS reports

USGS2016

Arsenite as an electron donor for anoxygenic photosynthesis: Description of three strains of Ectothiorhodospria from Mono Lake, California, and Big Soda Lake, Nevada

Three novel strains of photosynthetic bacteria from the family Ectothiorhodospiraceae were isolated from soda lakes of the Great Basin Desert, USA by employing arsenite (As(III)) as the sole electron donor in the enrichment/isolation process. Strain PHS-1 was previously isolated from a hot spring in Mono Lake, while strain MLW-1 was obtained from Mono Lake sediment, and strain BSL-9 was isolated from Big Soda Lake. Strains PHS-1, MLW-1, and BSL-9 were all capable of As(III)-dependent growth via anoxygenic photosynthesis and contained homologs of arxA, but displayed different phenotypes. Comparisons were made with three related species: Ectothiorhodospira shaposhnikovii DSM 2111, Ectothiorhodospira shaposhnikovii DSM 243 T , and Halorhodospira halophila DSM 244. All three type cultures oxidized arsenite to arsenate but did not grow with As(III) as the sole electron donor. DNA–DNA hybridization indicated that strain PHS-1 belongs to the same species as Ect. shaposhnikovii DSM 2111 (81.1% sequence similarity), distinct from Ect. shaposhnikovii DSM 243 T (58.1% sequence similarity). These results suggest that the capacity for light-driven As(III) oxidation is a common phenomenon among purple photosynthetic bacteria in soda lakes. However, the use of As(III) as a sole electron donor to sustain growth via anoxygenic photosynthesis is confined to novel isolates that were screened for by this selective cultivation criterion.

Life Sciences↗

USGS2011

The hormonal control of osmoregulation in teleost fish

Hormones are critical to the physiological alterations necessary for ion homeostasis when fish move between freshwater and seawater. Cortisol promotes seawater acclimation through differentiation of salt-secreting mitochondrion-rich cells and ion transport proteins in the gill. The growth hormone/insulin-like growth factor I axis is also important in seawater acclimation and acts in synergy with cortisol. Prolactin (PRL) is important in freshwater acclimation through regulation of ion and water permeability in the gill, gut, and kidney. Cortisol also promotes ion uptake and may interact with PRL during freshwater acclimation. For many species of fish, growth hormone promotes acclimation to seawater, PRL promotes acclimation to freshwater, and cortisol interacts with both hormones, thus having a dual osmoregulatory function.

Life Sciences↗

USGS1968

Role of intestinal microflora in the degradation of DDT by rainbow trout ( Salmo gairdneri )

Though liver homogenates show apparent microsomal enzyme DDT-dehydrochlorinase activity, in the intact fish the intestinal microflora play a major role in DDT detoxication. Since the presence of this microflora in fish depends on the recent intake of food (12), the rate of detoxication and hence the toxicity of ingested DDT to the rainbow trout will probably depend somewhat on the available food supply.

Life Sciences↗