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Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus

C. L. Schultz·B. C. Lidgerding·P. E. McAllister·F. M. Hetrick
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Abstract

A hybridoma cell line that continually secretes antibody against infectious hematopoietic necrosis virus was developed and designated M-IHNV-W1. Antibodies in culture fluids were concentrated and purified by Protein A Sepharose CL-4B affinity chromatography. The immunoglobulin was isotyped as IgG 2b with a kappa light chain. The IgG was specific for infectious hematopoietic necrosis virus and had binding activity but low neutralizing activity. Little cross-reactivity occurred with viral hemorrhagic septicemia or infectious pancreatic necrosis viruses.

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C. L. Schultz, B. C. Lidgerding, P. E. McAllister, F. M. Hetrick. 1985. Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus. https://doi.org/10.3147/jsfp.20.339

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