Preparation of monolayer cell cultures from tissues of some lower vertebrates

K. Wolf; M. C. Quimby; E. A. Pyle; R.P. Dexter

doi:10.1126/science.132.3443.1890

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Preparation of monolayer cell cultures from tissues of some lower vertebrates

K. Wolf·M. C. Quimby·E. A. Pyle·R.P. Dexter

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Abstract

Cold trypsin dispersion at p H 7.2 was used to obtain cultivable cells and cell groups from tissues of six species of fresh-water bony fishes, a frog, and a turtle. The cells readily attached to glass and were capable of at least limited, and in some cases extended, division in media consisting of commercially available components.

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K. Wolf, M. C. Quimby, E. A. Pyle, R.P. Dexter. 1960. Preparation of monolayer cell cultures from tissues of some lower vertebrates. https://doi.org/10.1126/science.132.3443.1890

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