USGS · 1013958
Comparative sensitivities of diagnostic procedures used to detect bacterial kidney disease in salmonid fishes
Abstract
Kidney and spleen homogenates from each of 60 coho salmon ( Oncorhynchus kisutch ) and steelhead trout ( Salmo gairdneri ) were examined for detection of Renibacterium salmoninarum . The proportions of positives differed widely with the detection procedures used: in coho salmon, 5% were positive by the Gram-stain procedure, 10% by the direct fluorescent antibody test, 48% by bacteriological isolation, 65% by staphylococcal coagglutination, and 73% by counterimmunoelectrophoresis; in steelhead trout, 3% were positive by Gram-stain, 8.3% by fluorescent antibody, 17% by bacteriological isolation, and 67% by counterimmunoelectrophoresis. Renibacterium salmoninarum was not detected in either coho salmon or steelhead trout by immunodiffusion analysis.
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R. C. Cipriano, C. E. Starliper, J. H. Schachte. 1985. Comparative sensitivities of diagnostic procedures used to detect bacterial kidney disease in salmonid fishes. https://doi.org/10.7589/0090-3558-21.2.144
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