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Research about Wisconsin

Source-linked reports with geographic coverage including Wisconsin.

At least 379 records · Page 21Linked to original sources

The role of groundwater transport in aquatic mercury cycling

Mercury, which is transported globally by atmospheric pathways to remote aquatic environments, is a ubiquitous contaminant at very low (nanograms Hg per liter) aqueous concentrations. Until recently, however, analytical and sampling techniques were not available for freshwater systems to quantify the actual levels of mercury concentrations without introducing significant contamination artifacts. Four different sampling strategies were used to evaluate ground water flow as a mercury source and transport mechanism within aquatic systems. The sampling strategies employ ultraclean techniques to determine mercury concentrations in groundwater and pore water near Pallette Lake, Wisconsin. Ambient groundwater concentrations are about 2–4 ng Hg L −1 , whereas pore waters near the sediment/water interface average about 12 ng Hg L −1 , emphasizing the importance of biogeochemical processes near the interface. Overall, the groundwater system removes about twice as much mercury (1.5 g yr −1 ) as it contributes (0.7 g yr −1 ) to Pallette Lake. About three fourths of the groundwater mercury load is recycled, thought to be derived from the water column.

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Long-term hydrologic and biogeochemical responses of a soft water seepage lake in north central Wisconsin

Hydrologic and chemical budgets were determined for the period October 1980 through September 1988 for Vandercook Lake, a 43-ha soft water seepage lake located in predominantly sandy outwash in northern Wisconsin. The 1951–1980 mean annual precipitation for the lake, based on nearby National Weather Service (NWS) stations, was 825 mm; volume-weighted p H of bulk atmospheric deposition during the 8-year study averaged 4.7. From October 1980 through September 1983, annual precipitation was 108% of the 30-year NWS average, annual recharge was 144% of the 8-year study period average, and annual groundwater inflow to the lake ranged from 5 to 9% of the total inflow. From October 1986 through September 1988, annual precipitation was 90% of the NWS average, annual recharge was 30% of the study period average, and the lake received no groundwater inflow. During the study, the lake changed from a system whose buffering mechanism was significantly influenced by mineral weathering in inflowing groundwater to one dominated by in-lake sulfate reduction. The functional differences exhibited by this lake during the 8 years of study demonstrate the tenuousness with which conclusions based on shorter-term studies of similar systems must be considered.

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Estimating groundwater exchange with lakes: 1. The stable isotope mass balance method

Groundwater inflow and outflow contributions to the hydrologic budget of lakes can be determined using a stable isotope ( 18 O/ 16 O) mass balance method. The stable isotope method provides a way of integrating the spatial and temporal complexities of the flow field around a lake, thereby offering an appealing alternative to the traditional time and labor intensive methods using seepage meters and an extensive piezometer network. In this paper the method is applied to a lake in northern Wisconsin, demonstrating that it can be successfully applied to lakes in the upper midwest where thousands of similar lakes exist. Inflow and outflow rates calculated for the Wisconsin lake using the isotope mass balance method are 29 and 54 cm/yr, respectively, which compare well to estimates, derived independently using a three-dimensional groundwater flow and solute transport model, of 20 and 50 cm/yr. Such a favorable comparison lends confidence to the use of the stable isotope method to estimate groundwater exchange with lakes. In addition, utilization of stable isotopes in studies of groundwater-lake systems lends insight into mixing processes occurring in the unsaturated zone and in the aquifer surrounding the lake and verifies assumed flow paths based on head measurements in piezometers.

Wisconsin↗

Estimating groundwater exchange with lakes: 2. Calibration of a three-dimensional, solute transport model to a stable isotope plume

A three-dimensional groundwater flow and solute transport model was calibrated to a plume of water described by measurements of δ 18 O and used to calculate groundwater inflow and outflow rates at a lake in northern Wisconsin. The flow model was calibrated to observed hydraulic gradients and estimated recharge rates. Calibration of the solute transport submodel to the configuration of a stable isotope ( 18 O) plume in the contiguous aquifer on the downgradient side of the lake provides additional data to constrain the model. A good match between observed and simulated temporal variations in plume configuration indicates that the model closely simulated the dynamics of the real system. The model provides information on natural variations of rates of groundwater inflow, lake water outflow, and recharge to the water table. Inflow and outflow estimates compare favorably with estimates derived by the isotope mass balance method (Krabbenhoft et al., this issue). Model simulations agree with field observations that show groundwater inflow rates are more sensitive to seasonal variations in recharge than outflow.

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Response of aquatic macrophytes to human land use perturbations in the watersheds of Wisconsin lakes, U.S.A.

Aquatic macrophyte communities were assessed in 53 lakes in Wisconsin, U.S.A. along environmental and land use development gradients to determine effects human land use perturbations have on aquatic macrophytes at the watershed and riparian development scales. Species richness and relative frequency were surveyed in lakes from two ecoregions: the Northern Lakes and Forests Ecoregion and the Southeastern Wisconsin Till Plain Ecoregion. Lakes were selected along a gradient of watershed development ranging from undeveloped (i.e., forested), to agricultural to urban development. Land uses occurring in the watershed and in perimeters of different width (0–100, 0–200, 0–500, and 0–1000 m from shore, in the watershed) were used to assess effects on macrophyte communities. Snorkel and SCUBA were used to survey aquatic macrophyte species in 18 quadrats of 0.25 m 2 along 14 transects placed perpendicular to shore in each lake. Effects of watershed development (e.g., agriculture and/or urban) were tested at whole-lake (entire littoral zone) and near-shore (within 7 m of shore) scales using canonical correspondence analysis (CCA) and linear regression. Overall, species richness was negatively related to watershed development, while frequencies of individual species and groups differed in level of response to different land use perturbations. Effects of land use in the perimeters on macrophytes, with a few exceptions, did not provide higher correlations compared to land use at the watershed scale. In lakes with higher total watershed development levels, introduced species, particularly Myriophyllum spicatum , increased in abundance and native species, especially potamids, isoetids, and floating-leaved plants, declined in abundance. Correlations within the northern and southeastern ecoregions separately were not significant. Multivariate analyses suggested species composition is driven by environmental responses as well as human development pressures. Both water chemistry and land use variables loaded positively with the first CCA axis indicating that these factors are correlated. Land use pressures in Wisconsin are greater in the southeastern portion of the state where lakes have higher concentrations of water chemistry variables including alkalinity, conductivity, pH, calcium, magnesium, and nitrogen. This creates a complex gradient that influences species composition of macrophyte communities from lake to lake.

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Anomalous spawning of smallmouth bass in Nebish Lake, Wisconsin: Implications for early spawning and over-winter survival

We observed that the smallmouth bass ( Micropterus dolomieu ) population in Nebish Lake, Wisconsin spawned twice-once during the traditional spring period and then again in summer, well beyond the typical spawning season for north-temperate lakes. We documented this anomalous spawning behavior and compared the characteristics of smallmouth bass nests built during the two distinct spawning seasons. Smallmouth bass built 463 nests (110.2 nests/km shoreline) as water temperature was rising toward 20°C. During the summer spawning period, 24 nests (5.7 nests/km shoreline) were constructed between 11 August and 29 August as water temperature was falling. Nests built in summer were significantly farther from shore, in deeper water, farther from cover, larger in diameter, and built by larger males than in the spring.

Wisconsin↗

Linking process to pattern: estimating spatiotemporal dynamics of a wildlife epidemic from cross‐sectional data

Underlying dynamic event processes unfolding in continuous time give rise to spatiotemporal patterns that are sometimes observable at only a few discrete times. Such event processes may be modulated simultaneously over several spatial (e.g., latitude and longitude) and temporal (e.g., age, calendar time, and cohort) dimensions. The ecological challenge is to understand the dynamic latent processes that were integrated over several dimensions (space and time) to produce the observed pattern: a so‐called inverse problem. An example of such a problem is characterizing epidemiological rate processes from spatially referenced age‐specific prevalence data for a wildlife disease such as chronic wasting disease (CWD). With age‐specific prevalence data, the exact infection times are not observed, which complicates the direct estimation of rates. However, the relationship between the observed data and the unobserved rate variables can be described with likelihood equations. Typically, for problems with multiple timescales, the likelihoods are integral equations without closed forms. The complexity of the likelihoods often makes traditional maximum‐likelihood approaches untenable. Here, using seven years of hunter‐harvest prevalence data from the CWD epidemic in white‐tailed deer (Odocoileus virginianus) in Wisconsin, USA, we develop and explore a Bayesian approach that allows for a detailed examination of factors modulating the infection rates over space, age, and time, and their interactions. Our approach relies on the Bayesian ability to borrow strength from neighbors in both space and time. Synthesizing a number of areas of event time analysis (current‐status data, age/period/cohort models, Bayesian spatial shared frailty models), our general framework has very broad ecological applicability beyond disease prevalence data to a number of important ecological event time analyses, including general survival studies with multiple time dimensions for which existing methodology is limited. We observed strong associations of infection rates with age, gender, and location. The infection rate appears to be increasing with time. We could not detect growth hotspots, or location by time interactions, which suggests that spatial variation in infection rates is determined primarily by when the disease arrives locally, rather than how fast it grows. We emphasize assumptions and the potential consequences of their violations.

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Occurrence of avian Plasmodium and West Nile virus in culex species in Wisconsin

The occurrence of multiple pathogens in mosquitoes and birds could affect the dynamics of disease transmission. We collected adult Culex pipiens and Cx. restuans (Cx. pipiens/restuans hereafter) from sites in Wisconsin and tested them for West Nile virus (WNV) and for avian malaria (Plasmodium). Gravid Cx. pipiens/restuans were tested for WNV using a commercial immunoassay, the RAMP?? WNV test, and positive results were verified by reverse transcriptasepolymerase chain reaction. There were 2 WNV-positive pools of Cx. pipiens/restuans in 2006 and 1 in 2007. Using a bias-corrected maximum likelihood estimation, the WNV infection rate for Cx. pipiens/restuans was 5.48/1,000 mosquitoes in 2006 and 1.08/1,000 mosquitoes in 2007. Gravid Cx. pipiens or Cx. restuans were tested individually for avian Plasmodium by a restriction enzymebased assay. Twelve mosquitoes were positive for avian Plasmodium (10.0), 2 were positive for Haemoproteus, and 3 were positive for Leucocytozoon. There were 4 mixed infections, with mosquitoes positive for >1 of the hemosporidian parasites. This work documents a high rate of hemosporidian infection in Culex spp. and illustrates the potential for co-infections with other arboviruses in bird-feeding mosquitoes and their avian hosts. In addition, hemosporidian infection rates may be a useful tool for investigating the ecological dynamics of Culex/avian interactions. ?? 2010 by The American Mosquito Control Association, Inc.

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Parking lot runoff quality and treatment efficiency of a stormwater-filtration device, Madison, Wisconsin, 2005-07

To evaluate the treatment efficiency of a stormwater-filtration device (SFD) for potential use at Wisconsin Department of Transportation (WisDOT) park-and-ride facilities, a SFD was installed at an employee parking lot in downtown Madison, Wisconsin. This type of parking lot was chosen for the test site because the constituent concentrations and particle-size distributions (PSDs) were expected to be similar to those of a typical park-and-ride lot operated by WisDOT. The objective of this particular installation was to reduce loads of total suspended solids (TSS) in stormwater runoff to Lake Monona. This study also was designed to provide a range of treatment efficiencies expected for a SFD. Samples from the inlet and outlet were analyzed for 33 organic and inorganic constituents, including 18 polycyclic aromatic hydrocarbons (PAHs). Samples were also analyzed for physical properties, including PSD. Water-quality samples were collected for 51 runoff events from November 2005 to August 2007. Samples from all runoff events were analyzed for concentrations of suspended sediment (SS). Samples from 31 runoff events were analyzed for 15 constituents, samples from 15 runoff events were analyzed for PAHs, and samples from 36 events were analyzed for PSD. The treatment efficiency of the SFD was calculated using the summation of loads (SOL) and the efficiency ratio methods. Constituents for which the concentrations and (or) loads were decreased by the SFD include TSS, SS, volatile suspended solids, total phosphorous (TP), total copper, total zinc, and PAHs. The efficiency ratios for these constituents are 45, 37, 38, 55, 22, 5, and 46 percent, respectively. The SOLs for these constituents are 32, 37, 28, 36, 23, 8, and 48 percent, respectively. The SOL for chloride was -21 and the efficiency ratio was -18. Six chemical constituents or properties-dissolved phosphorus, chemical oxygen demand, dissolved zinc, total dissolved solids, dissolved chemical oxygen demand, and dissolved copper-were not included in the efficiency or SOL, because the difference between concentrations in samples from the inlet and outlet were not significant. Concentrations of TP and TSS were inexplicably high in samples at the inlet for one event.

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Biological water-quality assessment of selected streams in the Milwaukee Metropolitan Sewerage District Planning Area of Wisconsin, 2007

Changes in the water quality of stream ecosystems in an urban area may manifest in conspicuous ways, such as in murky or smelly streamwater, or in less conspicuous ways, such as fewer native or pollution-sensitive organisms. In 2004, and again in 2007, the U.S. Geological Survey sampled stream organisms—algae, invertebrates, and fish—in 14 Milwaukee area streams to assess water quality as part of the ongoing Milwaukee Metropolitan Sewerage District (MMSD) Corridor Study. In addition, passive-sampling devices (SPMDs, “semipermeable membrane devices”) were deployed at a subset of sites in order to evaluate the potential exposure of stream organisms to certain toxic chemicals. Results of the 2007 sampling effort are the focus of this report. Results of sampling from 2007 are compared with results from 2004. The water quality of sampled streams was assessed by evaluating biological-assemblage data, metrics computed from assemblage data, and an aggregate bioassessment ranking method that combined data for algae, invertebrates, and fish. These data contain information about the abundance (number) of different species in each group of stream organisms and the balance between species that can or cannot tolerate polluted or disturbed conditions. In 2007, the highest numbers of algal, invertebrate, and fish species were found at the Milwaukee River at Milwaukee, the largest sampled site. Algal results indicated water quality concerns at 10 of the 14 sampled sites due to the occurrence of nuisance algae or low percentages of pollution-sensitive algae. When compared to 2004, total algal biovolume was higher in 2007 at 12 of 14 sites, due mostly to more nuisance green algae from unknown causes. Results of several metrics, including the Hilsenhoff Biotic Index (HBI-10), suggest that invertebrate assemblages in the Little Menomonee River, Underwood Creek, and Honey Creek were poorer quality in 2007 compared to 2004. Six sites received “very poor” quality ratings for fish in 2007, mostly because inadequate numbers of fish were collected at five sites to allow computation of an Index of Biotic Integrity (IBI); this resulted in three additional sites receiving “very poor” ratings compared to 2004. Some signs of potential improvement in the fish assemblage were evident at Lincoln Creek, possibly reflecting delayed effects of the restoration of stream habitat, completed in 2002; however, algae and invertebrates did not show signs of improvement. Aggregate bioassessment rankings across all groups of organisms for 2004 and 2007 indicated that water quality at the two Milwaukee River main stem sites (at Milwaukee and near Cedarburg), Jewel Creek, and the Menomonee River at Menomonee Falls was the least-degraded among all sampled sites. Rankings for Oak Creek and Little Menomonee suggested water quality was worse in 2007 compared to 2004 and placed these two sites together with Kinnickinnic River and Underwood Creek, two concrete-line sites, indicating the most-degraded water quality among all sampled sites. The aggregate ranking for Lincoln Creek in 2007 would have placed it in the most-degraded category but for the positive influence of the fish ranking when compared to poor algal and invertebrate rankings. Potential toxicity due to certain manmade chemicals, such as polycyclic aromatic hydrocarbons (PAHs), was found at all six sites where SPMDs were deployed. As was found in 2004, the highest potential toxicity in 2007 was observed at Lincoln Creek where chemical screening in 2007 also showed the highest total PAHs of all six sites; however, potential toxicity at Little Menomonee River, Honey Creek, and Kinnickinnic River was relatively high compared to Milwaukee River near Cedarburg. Although toxicity and chemical results in 2007 did not agree with aggregate rankings for Lincoln Creek because of fish, nor for Honey Creek, the results did agree with aggregate rankings at four of the six sites. In addition to toxicological and chemical influences, the more urbanized sites have high percentages of impervious surface area, resulting in frequent high stream flows that can adversely affect algal, invertebrate, and fish assemblages. Assessments of the ecological status of different groups of organisms and of potential chemical and physical stressors to organisms are important tools in evaluating streamwater quality.

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Hydrology, water quality, and response to changes in phosphorus loading of Minocqua and Kawaguesaga Lakes, Oneida County, Wisconsin, with special emphasis on effects of urbanization

Minocqua and Kawaguesaga Lakes are 1,318- and 690-acre interconnected lakes in the popular recreation area of north-central Wisconsin. The lakes are the lower end of a complex chain of lakes in Oneida and Vilas Counties, Wis. There is concern that increased stormwater runoff from rapidly growing residential/commercial developments and impervious surfaces from the urbanized areas of the Town of Minocqua and Woodruff, as well as increased effluent from septic systems around their heavily developed shoreline has increased nutrient loading to the lakes. Maintaining the quality of the lakes to sustain the tourist-based economy of the towns and the area was a concern raised by the Minocqua/Kawaguesaga Lakes Protection Association. Following several small studies, a detailed study during 2006 and 2007 was done by the U.S. Geological Survey, in cooperation with the Minocqua/Kawaguesaga Lakes Protection Association through the Town of Minocqua to describe the hydrology and water quality of the lakes, quantify the sources of phosphorus including those associated with urban development and to better understand the present and future effects of phosphorus loading on the water quality of the lakes. The water quality of Minocqua and Kawaguesaga Lakes appears to have improved since 1963, when a new sewage-treatment plant was constructed and its discharge was bypassed around the lakes, resulting in a decrease in phosphorus loading to the lakes. Since the mid-1980s, the water quality of the lakes has changed little in response to fluctuations in phosphorus loading from the watershed. From 1986 to 2009, summer average concentrations of near-surface total phosphorus in the main East Basin of Minocqua Lake fluctuated from 0.009 mg/L to 0.027 mg/L but generally remained less than 0.022 mg/L, indicating that the lake is mesotrophic. Phosphorus concentrations from 1988 through 1996, however, were lower than the long-term average, possibly the result of an extended drought in the area. Water‑quality data for Kawaguesaga Lake had a similar pattern to that of Minocqua Lake. Summer average chlorophyll a concentrations and Secchi depths also indicate that the lakes generally are mesotrophic but occasionally borderline eutrophic, with no long-term trends. During the study, major water and phosphorus sources were measured directly, and minor sources were estimated to construct detailed water and phosphorus budgets for the lakes for monitoring years (MY) 2006 and 2007. During these years, the Minocqua Thoroughfare contributed about 38 percent of the total inflow to the lakes, and Tomahawk Thoroughfare contributed 34 percent; near-lake inflow, precipitation, and groundwater contributed about 1, 16, and 11 percent of the total inflow, respectively. Water leaves the lakes primarily through the Tomahawk River outlet (83 percent) or by evaporation (14 percent), with minor outflow to groundwater. Total input of phosphorus to both lakes was about 3,440 pounds in MY 2006 and 2,200 pounds in MY 2007. The largest sources of phosphorus entering the lakes were the Minocqua and Tomahawk Thoroughfares, which delivered about 39 and 26 percent of the total, respectively. The near-lake drainage area, containing most of the urban and residential developments, disproportionately accounted for about 12 percent of the total phosphorus input but only about 1 percent of the total water input (estimated with WinSLAMM). The next largest contributions were from septic systems and precipitation, each contributing about 10 percent, whereas groundwater delivered about 4 percent of the total phosphorus input. Empirical lake water-quality models within BATHTUB were used to simulate the response of Minocqua and Kawaguesaga Lakes to 19 phosphorus-loading scenarios. These scenarios included the current base years (2006–07) for which lake water quality and loading were known, nine general increases or decreases in phosphorus loading from controllable external sources (inputs from the tributaries and nearshore areas around the lakes and input from septic systems), and nine scenarios corresponding to future changes in phosphorus loading from residential and urban development, referred to as “2030 buildout,” and removal of septic system inputs. The 2030 buildout scenario with existing stormwater controls resulted in a degradation in water quality: phosphorus concentrations increased by about 0.001 mg/L, chlorophyll a concentrations increased by 0.2–0.8 μg/L, and Secchi depths decreased slightly. The largest degradation in water quality was estimated to occur in Kawaguesaga Lake. If 2030 buildout occurred with implementation of best management practices to achieve a 50-percent reduction in loading from near-lake drainages, it is possible that water quality would change very little from existing conditions. Numerous noncontributing areas exist within the watershed that help minimize surface runoff and nutrient loading to the lakes; however, if future development included extending or connecting drainage from these areas into the lakes, loading to the lakes could greatly increase and cause a degradation in the water quality of the lakes. Simulations of removal of phosphorus loading from septic systems around Minocqua Lake improved the water quality of the lakes: in simulations for that scenario, phosphorus concentrations decreased by about 0.001 mg/L, chlorophyll a concentrations decreased by 0.5–0.7 μg/L, and Secchi depths increased by 0.3–0.7 ft. If all controllable external phosphorus loading could be reduced by 50 percent, the lakes would become oligotrophic with respect to phosphorus concentration but would still remain mesotrophic with respect to chlorophyll a concentration and Secchi depth. Improvements in the water quality of the lakes are likely only with a combination of management actions that decrease inputs from the developed near-lake drainage areas and from septic systems.

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Polychlorinated biphenyls as probes of biogeochemical processes in rivers

A field study was conducted to investigate the use of PCB (polychlorinated biphenyl) congener and homolog assemblages as tracers of biogeochemical processes in the Milwaukee and Manitowoc Rivers in southeastern Wisconsin from 1993 to 1995. PCB congeners in the dissolved and suspended particle phases, along with various algal indicators (algal carbon and pigments), were quantitated in the water seasonally. In addition, PCB congener assemblages were determined seasonally in surficial bed sediments. Biogeochemical processes investigated included: determination of the source of suspended particles and bottom sediments by comparison with known Aroclor mixtures, water-solid partitioning, and algal uptake of PCBs. Seasonal differences among the PCB assemblages were observed mainly in the dissolved phase, somewhat less in the suspended particulate phase, and not at all in the bed sediments.

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Cycling of mercury across the sediment-water interface in seepage lakes: Chapter 13, Advances in Chemistry

The magnitude and direction of Hg fluxes across the sediment—water interface were estimated by groundwater, dry bulk sediment, sediment pore water, sediment trap, and water-column analyses in two northern Wisconsin seepage lakes. Little Rock Lake (Treatment Basin) received no groundwater discharge during the study period (1988—1990), and Follette Lake received continuous groundwater discharge. In Little Rock Lake, settling of particulate matter accounted for the major Hg delivery mechanism to the sediment—water interface. Upward diffusion of Hg from sediment pore waters below 2—4-cm sediment depth was apparently a minor source during summer stratification. Time-series comparisons suggested that the observed buildup of Hg in the hypolimnion of Little Rock Lake was attributable to dissolution and diffusion of Hg from recently fallen particulate matter close to the sediment—water interface. Groundwater inflow represented an important source of new Hg, and groundwater outflow accounted for significant removal of Hg from Pallette Lake. Equilibrium speciation calculations revealed that association of Hg with organic matter may control solubility in well-oxygenated waters, whereas in anoxic environments sulfur (polysulfide and bisulfide) complexation governs dissolved total Hg levels.

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Benthic biogeochemical cycling, nutrient stoichiometry, and carbon and nitrogen mass balances in a eutrophic freshwater bay

Green Bay, while representing only ~7% of the surface area and ~1.4% of the volume of Lake Michigan, contains one‐third of the watershed of the lake, and receives approximately one‐third of the total nutrient loading to the Lake Michigan basin, largely from the Fox River at the southern end of the bay. With a history of eutrophic conditions dating back nearly a century, the southern portion of the bay behaves as an efficient nutrient and sediment trap, sequestering much of the annual carbon and nitrogen input within sediments accumulating at up to 1 cm per year. Depositional fluxes of organic matter varied from ~0.1 mol C m22 yr21 to >10 mol C m −2 yr −1 and were both fairly uniform in stoichiometric composition and relatively labile. Estimates of benthic recycling derived from pore‐water concentration gradients, whole‐sediment incubation experiments, and deposition‐burial models of early diagenesis yielded an estimated 40% of the carbon and 50% of the nitrogen recycled back into the overlying water. Remineralization was relatively rapid with ~50% of the carbon remineralized within ~15 yr of deposition, and a mean residence time for metabolizable carbon and nitrogen in the sediments of 20 yr. On average, organic carbon regeneration occurred as 75% CO 2 , 15% CH 4 , and 10% dissolved organic carbon (DOC). Carbon and nitrogen budgets for the southern bay were based upon direct measurements of inputs and burial and upon estimates of export and production derived stoichiometrically from a coupled phosphorus budget. Loadings of organic carbon from rivers were ~3.7 mol m −2 yr −1 , 80% in the form of DOC and 20% as particulate organic carbon. These inputs were lost through export to northern Green Bay and Lake Michigan (39%), through sediment burial (26%), and net CO 2 release to the atmosphere (35%). Total carbon input, including new production, was 4.54 mol m −2 yr −1 , equivalent to ~10% of the gross annual primary production. Nitrogen budget terms were less well quantified, with nitrogen export ~54% of total inputs and burial ~24%, leaving an unquantified residual loss term in the nitrogen budget of ~22%.

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The application of an analytic element model to investigate groundwater-lake interactions at Pretty Lake, Wisconsin

Pretty Lake is a 64 acre, sandy-bottomed groundwater flow-through lake that has a history of hydrologic disturbance. Residents and regulators require a better understanding of lake-groundwater interaction to develop measures to protect the lake's hydrologic system and water quality. A groundwater flow model was constructed as a tool to synthesize field data collected at the site, delineate recharge areas that supply groundwater to the lake, and predict die effect of dredging an adjacent drainage ditch. The one layer, two-dimensional steady-state areal model used analytic element (AE) methods because they are quick to apply and include sophisticated simulation of groundwater-surface water interaction. The model calibrated well to groundwater heads (mean absolute difference = 0.05 m), lake stage (within 0.05 m) and ditch fluxes (mean absolute difference = 0.0023 m 3 ·s −1 ). Model results showed that a single 1000 m wide recharge area supplies all the groundwater inflow to the lake. In addition, the model predicted that dredging an adjacent ditch by 3.0 m would lower the lake level by 0.31 m. The analytic element model was verified using a widely accepted finite-difference (FD) code; differences were less than ±0.015 m near die lake area and reached a maximum of 0.08 m at far corners of the FD grid. These differences are likely a result of die nodal interpolation inherent to FD techniques and error associated with applying a discrete boundary to die AE infinite aquifer. Although developed recently, AE methods have great potential to aid characterizations of groundwater-lake systems.

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Rehabilitation of Delavan Lake, Wisconsin

A comprehensive rehabilitation plan was developed and implemented to shift Delavan Lake , Wisconsin, from a hypereutrophic to a mesotrophic condition. The plan was threefold: (1) reduce external phosphorus (P) loading by applying Best Management Practices in the watershed, enhance an existing wetland, and short-circuit the inflows through the lake , (2) reduce internal P loading by treating the sediments with alum and removing carp, and (3) rehabilitate the fishery by removing carp and bigmouth buffalo and adding piscivores (biomanipulation). The first and second parts of the plan met with only limited success. With only minor reductions in internal and external P loading, P concentrations in the lake returned to near pre-treatment concentrations. The intensive biomanipulation and resulting trophic cascade (increased piscivores, decreased planktivores, increased large zooplankton populations, and reduced phytoplankton populations) eliminated most of the original problems in the lake (blue-green algal blooms and limited water clarity). However, now there is extensive macrophyte growth and abundant filamentous algae. Without significantly reducing the sources of the problems (high P loading) in Delavan Lake , the increased water clarity may not last. With an improved understanding of the individual components of this rehabilitation program, better future management plans can be developed for Delavan Lake and other lakes and reservoirs with similar eutrophication problems.

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Wildfire risk in the wildland-urban interface: A simulation study in northwestern Wisconsin

The rapid growth of housing in and near the wildland–urban interface (WUI) increases wildfire risk to lives and structures. To reduce fire risk, it is necessary to identify WUI housing areas that are more susceptible to wildfire. This is challenging, because wildfire patterns depend on fire behavior and spread, which in turn depend on ignition locations, weather conditions, the spatial arrangement of fuels, and topography. The goal of our study was to assess wildfire risk to a 60,000 ha WUI area in northwestern Wisconsin while accounting for all of these factors. We conducted 6000 simulations with two dynamic fire models: Fire Area Simulator (FARSITE) and Minimum Travel Time (MTT) in order to map the spatial pattern of burn probabilities. Simulations were run under normal and extreme weather conditions to assess the effect of weather on fire spread, burn probability, and risk to structures. The resulting burn probability maps were intersected with maps of structure locations and land cover types. The simulations revealed clear hotspots of wildfire activity and a large range of wildfire risk to structures in the study area. As expected, the extreme weather conditions yielded higher burn probabilities over the entire landscape, as well as to different land cover classes and individual structures. Moreover, the spatial pattern of risk was significantly different between extreme and normal weather conditions. The results highlight the fact that extreme weather conditions not only produce higher fire risk than normal weather conditions, but also change the fine-scale locations of high risk areas in the landscape, which is of great importance for fire management in WUI areas. In addition, the choice of weather data may limit the potential for comparisons of risk maps for different areas and for extrapolating risk maps to future scenarios where weather conditions are unknown. Our approach to modeling wildfire risk to structures can aid fire risk reduction management activities by identifying areas with elevated wildfire risk and those most vulnerable under extreme weather conditions.

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Genetic susceptibility to chronic wasting disease in free-ranging white-tailed deer: complement component C1q and Prnp polymorphisms

The genetic basis of susceptibility to chronic wasting disease (CWD) in free-ranging cervids is of great interest. Association studies of disease susceptibility in free-ranging populations, however, face considerable challenges including: the need for large sample sizes when disease is rare, animals of unknown pedigree create a risk of spurious results due to population admixture, and the inability to control disease exposure or dose. We used an innovative matched case&ndash;control design and conditional logistic regression to evaluate associations between polymorphisms of complement C1q and prion protein (Prnp) genes and CWD infection in white-tailed deer from the CWD endemic area in south-central Wisconsin. To reduce problems due to admixture or disease-risk confounding, we used neutral genetic (microsatellite) data to identify closely related CWD-positive ( n = 68) and CWD-negative ( n = 91) female deer to serve as matched cases and controls. Cases and controls were also matched on factors (sex, location, age) previously demonstrated to affect CWD infection risk. For Prnp, deer with at least one Serine (S) at amino acid 96 were significantly less likely to be CWD-positive relative to deer homozygous for Glycine (G). This is the first characterization of genes associated with the complement system in white-tailed deer. No tests for association between any C1q polymorphism and CWD infection were significant at p < 0.05. After controlling for Prnp, we found weak support for an elevated risk of CWD infection in deer with at least one Glycine (G) at amino acid 56 of the C1qC gene. While we documented numerous amino acid polymorphisms in C1q genes none appear to be strongly associated with CWD susceptibility.

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