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Research about Virginia

Source-linked reports with geographic coverage including Virginia.

At least 343 records · Page 19Linked to original sources
USGS2003

Assessment of Late Pleistocene to recent climate-induced vegetation changes in and near Shenandoah National Park (Blue Ridge Province, VA)

Pollen evidence from a shallow core in the Blue Ridge Mountains (Big Meadows, Virginia) and from other outcrops in and adjacent to Shenandoah National Park, indicates that from the Late Pleistocene through the Holocene (45-0 ka) regional vegetation in north-central Virginia fluctuated from warm temperate forests to fully developed taiga (boreal forest). Present day analogues to these vegetation zones can be found in the forests and forest transitions extending from central Georgia (approximately 32° N latitude) to central Ontario, Labrador, and northern Newfoundland (approximately 52-55° N latitude). It is still undetermined (on the basis of our pollen evidence) whether it was ever cold enough in the study area during this 45,000-year time interval to develop alpine tundra extensively along these ridge tops. Current evidence from the study area suggests that the forests in and around Shenandoah National Park changed frequently in composition through the studied time interval. Most of the forests previously established in the study area were of types that favored notably cooler mean annual temperatures than the forest type that is established currently in the proximity of the Park. Although this fossil record is a partial one, the radiometric carbon evidence verifies that we now have discovered pollen assemblages of full-glacial age (last glacial maximum) in the Blue Ridge, of multiple forest types.

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USGS2003

Use of the Hydrological Simulation Program-FORTRAN and bacterial source tracking for development of the fecal coliform total maximum daily load (TMDL) for Accotink Creek, Fairfax County, Virginia

Impairment of surface waters by fecal coliform bacteria is a water-quality issue of national scope and importance. Section 303(d) of the Clean Water Act requires that each State identify surface waters that do not meet applicable water-quality standards. In Virginia, more than 175 stream segments are on the 1998 Section 303(d) list of impaired waters because of violations of the water-quality standard for fecal coliform bacteria. A total maximum daily load (TMDL) will need to be developed by 2006 for each of these impaired streams and rivers by the Virginia Departments of Environmental Quality and Conservation and Recreation. A TMDL is a quantitative representation of the maximum load of a given water-quality constituent, from all point and nonpoint sources, that a stream can assimilate without violating the designated water-quality standard. Accotink Creek, in Fairfax County, Virginia, is one of the stream segments listed by the State of Virginia as impaired by fecal coliform bacteria. Watershed modeling and bacterial source tracking were used to develop the technical components of the fecal coliform bacteria TMDL for Accotink Creek. The Hydrological Simulation Program-FORTRAN (HSPF) was used to simulate streamflow, fecal coliform concentrations, and source-specific fecal coliform loading in Accotink Creek. Ribotyping, a bacterial source tracking technique, was used to identify the dominant sources of fecal coliform bacteria in the Accotink Creek watershed. Ribotyping also was used to determine the relative contributions of specific sources to the observed fecal coliform load in Accotink Creek. Data from the ribotyping analysis were incorporated into the calibration of the fecal coliform model. Study results provide information regarding the calibration of the streamflow and fecal coliform bacteria models and also identify the reductions in fecal coliform loads required to meet the TMDL for Accotink Creek. The calibrated streamflow model simulated observed streamflow characteristics with respect to total annual runoff, seasonal runoff, average daily streamflow, and hourly stormflow. The calibrated fecal coliform model simulated the patterns and range of observed fecal coliform bacteria concentrations. Observed fecal coliform bacteria concentrations during low-flow periods ranged from 25 to 800 colonies per 100 milliliters, and peak concentrations during storm-flow periods ranged from 19,000 to 340,000 colonies per 100 milliliters. Simulated source-specific contributions of fecal coliform bacteria to instream load were matched to the observed contributions from the dominant sources, which were cats, deer, dogs, ducks, geese, humans, muskrats, and raccoons. According to model results, an 89-percent reduction in the current fecal coliform load delivered from the watershed to Accotink Creek would result in compliance with the designated water-quality goals and associated TMDL.

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USGS2003

Use of the Hydrological Simulation Program-FORTRAN and bacterial source tracking for development of the fecal coliform total maximum daily load (TMDL) for Blacks Run, Rockingham County, Virginia

Impairment of surface waters by fecal coliform bacteria is a water-quality issue of national scope and importance. Section 303(d) of the Clean Water Act requires that each State identify surface waters that do not meet applicable water-quality standards. In Virginia, more than 175 stream segments are on the 1998 Section 303(d) list of impaired waters because of violations of the water-quality standard for fecal coliform bacteria. A total maximum daily load (TMDL) will need to be developed by 2006 for each of these impaired streams and rivers by the Virginia Departments of Environmental Quality and Conservation and Recreation. A TMDL is a quantitative representation of the maximum load of a given water-quality constituent, from all point and nonpoint sources, that a stream can assimilate without violating the designated water-quality standard. Blacks Run, in Rockingham County, Virginia, is one of the stream segments listed by the State of Virginia as impaired by fecal coliform bacteria. Watershed modeling and bacterial source tracking were used to develop the technical components of the fecal coliform bacteria TMDL for Accotink Creek. The Hydrological Simulation Program-FORTRAN (HSPF) was used to simulate streamflow, fecal coliform concentrations, and source-specific fecal coliform loading in Blacks Run. Ribotyping, a bacterial source tracking technique, was used to identify the dominant sources of fecal coliform bacteria in the Blacks Run watershed. Ribotyping also was used to determine the relative contributions of specific sources to the observed fecal coliform load in Blacks Run. Data from the ribotyping analysis were incorporated into the calibration of the fecal coliform model. Study results provide information regarding the calibration of the streamflow and fecal coliform bacteria models and also identify the reductions in fecal coliform loads required to meet the TMDL for Blacks Run. The calibrated streamflow model simulated observed streamflow characteristics with respect to total annual runoff, seasonal runoff, average daily streamflow, and hourly stormflow. The calibrated fecal coliform model simulated the patterns and range of observed fecal coliform bacteria concentrations. Observed fecal coliform bacteria concentrations during low-flow periods ranged from 40 to 7,000 colonies per 100 milliliters, and peak concentrations during storm-flow periods ranged from 33,000 to 260,000 colonies per 100 milliliters. Simulated source-specific contributions of fecal coliform bacteria to instream load were matched to the observed contributions from the dominant sources, which were cats, cattle, deer, dogs, ducks, geese, horses, humans, muskrats, poultry, raccoons, and sheep. According to model results, a 95-percent reduction in the current fecal coliform load delivered from the watershed to Blacks Run would result in compliance with the designated water-quality goals and associated TMDL.

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USGS2003

Use of the Hydrological Simulation Program-FORTRAN and bacterial source tracking for development of the fecal coliform total maximum daily load (TMDL) for Christians Creek, Augusta County, Virginia

Impairment of surface waters by fecal coliform bacteria is a water-quality issue of national scope and importance. Section 303(d) of the Clean Water Act requires that each State identify surface waters that do not meet applicable water-quality standards. In Virginia, more than 175 stream segments are on the 1998 Section 303(d) list of impaired waters because of violations of the water-quality standard for fecal coliform bacteria. A total maximum daily load (TMDL) will need to be developed by 2006 for each of these impaired streams and rivers by the Virginia Departments of Environmental Quality and Conservation and Recreation. A TMDL is a quantitative representation of the maximum load of a given water-quality constituent, from all point and nonpoint sources, that a stream can assimilate without violating the designated water-quality standard. Christians Creek, in Augusta County, Virginia, is one of the stream segments listed by the State of Virginia as impaired by fecal coliform bacteria. Watershed modeling and bacterial source tracking were used to develop the technical components of the fecal coliform bacteria TMDL for Christians Creek. The Hydrological Simulation Program-FORTRAN (HSPF) was used to simulate streamflow, fecal coliform concentrations, and source-specific fecal coliform loading in Christians Creek. Ribotyping, a bacterial source tracking technique, was used to identify the dominant sources of fecal coliform bacteria in the Christians Creek watershed. Ribotyping also was used to determine the relative contributions of specific sources to the observed fecal coliform load in Christians Creek. Data from the ribotyping analysis were incorporated into the calibration of the fecal coliform model. Study results provide information regarding the calibration of the streamflow and fecal coliform bacteria models and also identify the reductions in fecal coliform loads required to meet the TMDL for Christians Creek. The calibrated streamflow model simulated observed streamflow characteristics with respect to total annual runoff, seasonal runoff, average daily streamflow, and hourly stormflow. The calibrated fecal coliform model simulated the patterns and range of observed fecal coliform bacteria concentrations. Observed fecal coliform bacteria concentrations during low-flow periods ranged from 40 to 2,000 colonies per 100 milliliters, and peak concentrations during stormflow periods ranged from 23,000 to 730,000 colonies per 100 milliliters. Additionally, fecal coliform bacteria concentrations were generally higher upstream and lower downstream. Simulated source-specific contributions of fecal coliform bacteria to instream load were matched to the observed contributions from the dominant sources, which were beaver, cats, cattle, deer, dogs, ducks, geese, horses, humans, muskrats, poultry, raccoons, and sheep. According to model results, a 96-percent reduction in the current fecal coliform load delivered from the watershed to Christians Creek would result in compliance with the designated water-quality goals and associated TMDL.

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USGS2003

Aquifer susceptibility in Virginia, 1998-2000

The U.S. Geological Survey (USGS), in cooperation with the Virginia Department of Health, sampled water from 171 wells and springs across the Commonwealth of Virginia between 1998 and 2000 as part of the Virginia Aquifer Susceptibility study. Most of the sites sampled are public water supplies that are part of the comprehensive Source Water Assessment Program for the Commonwealth. The fundamental premise of the study was that the identification of young waters (less than 50 years) by multiple environmental tracers could be used as a guide for classifying aquifers in terms of susceptibility to contamination from near-surface sources. Environmental tracers, including chlorofluorocarbons (CFCs), sulfur hexafluoride (SF6), tritium (3H), and tritium/helium-3 (3H/3He), and carbon isotopes (14C and d13C) were used to determine the age of water discharging from wells and springs. Concentrations of CFCs greater than 5 picograms per kilogram and 3H concentrations greater than 0.6 tritium unit were used as thresholds to indicate that parts of the aquifer sampled have a component of young water and are, therefore, susceptible to near-surface contamination. Concentrations of CFCs exceeded the susceptibility threshold in 22 percent of the wells and in one spring sampled in the Coastal Plain regional aquifer systems. About 74 percent of the samples from wells with the top of the first water zone less than 100 feet below land surface exceeded the threshold values, and water supplies developed in the upper 100 feet of the Coastal Plain are considered to be susceptible to contamination from near-surface sources. The maximum depth to the top of the screened interval for wells that contained CFCs was less than 150 feet. Wells completed in the deep confined aquifers in the Coastal Plain generally contain water older than 1,000 years, as indicated by carbon-14 dating, and are not considered to be susceptible to contamination under natural conditions. All of the water samples from wells and springs in the fractured-rock terrains (the Appalachian Plateaus, Valley and Ridge, Blue Ridge, and Piedmont regional aquifer systems) contained concentrations of CFCs and 3H greater than one or both of the thresholds. Because all of the water samples exceeded at least one of the threshold values, young water is present throughout most of these regional aquifer systems; therefore, water supplies developed in these systems are susceptible to contamination from near-surface sources. No relation between well depth and presence of CFCs is evident from samples in the fractured-rock terrains. More than 95 percent of the samples for which the dating methods were applicable contained waters with apparent ages less than 35 years. About 5 percent of these samples, most of which were from the Blue Ridge and Piedmont regional aquifer systems, contained young waters with apparent ages of less than 5 years. Most of the samples from the Valley and Ridge Carbonate, Blue Ridge, and Piedmont regional aquifer systems had young water fractions of more than 50 percent, whereas samples from the Coastal Plain Shallow and Appalachian Plateaus regional aquifer systems contained less than 40 percent young waters. Concentrations of CFCs in excess of air-water equilibrium, which can indicate that nonatmospheric sources (such as sewage effluent) have introduced CFCs into the ground-water system, were measured in 6 and 48 percent of the water samples from the Coastal Plain and fractured-rock regional aquifer systems, respectively. The nitrate (NO3) concentrations greater than the USGS detection level of 0.05 milligrams per liter generally increase as the apparent age of the young water fraction decreases, with the highest NO3 concentrations for samples in which one or more of the CFCs are above modern atmospheric mixing ratios (commonly referred to as 'contaminated' for ground-water dating purposes).

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USGS2003

Water resources management plan, Richmond National Battlefield Park, Virginia

Richmond National Battlefield Park (Richmond NBP) consists of 1,366 acres in 11 geographically separate units that are located primarily east, northeast, and southeast of the city of Richmond, Virginia. This Water Resources Management Plan addresses nine of the units: Beaver Dam Creek, Chickahominy Bluff, Cold Harbor (including the Garthright House), Drewry’s Bluff, Fort Harrison, Gaines’ Mill, and Glendale and Malvern Hill. The units are in the Atlantic Coastal Plain Physiographic Province between the James and York rivers. The small streams that drain each of the units are tributaries of either the Chickahominy River or James River and ultimately contribute to the Chesapeake Bay.

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USGS2002

Anthropogenic sources of arsenic and copper to sediments in a suburban lake, Northern Virginia

Mass balances of total arsenic and copper for a suburban lake in densely populated northern Virginia were calculated using data collected during 1998. Mass-balance terms were precipitation; stream inflow, including road runoff; stream outflow; and contributions from leaching of pressure-treated lumber. More mass of arsenic and copper was input to the lake than was output; the 1998 lake-retention rates were 70% for arsenic and 20% for copper. The arsenic mass balance compared well with a calculated annual mass accumulation in the top 1 cm of the lake sediments; however, the calculated contribution of copper to the lake was insufficient to account for the amount of copper in this zone. Leaching experiments were conducted on lumber treated with chromated copper arsenate (CCA) to quantify approximate amounts of arsenic and copper contributed by this source. Sources to lake sediments included leaching of CCA-treated lumber (arsenic, 50%; copper, 4%), streamwater (arsenic, 50%; copper, 90%), and atmospheric deposition (arsenic, 1%; copper, 3%). Results of this study suggest that CCA-treated lumber and road runoff could be significant nonpoint sources of arsenic and copper, respectively, in suburban catchments.

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USGS2002

Intercontinental convergence of stream fish community traits along geomorphic and hydraulic gradients

Community convergence across biogeographically distinct regions suggests the existence of key, repeated, evolutionary mechanisms relating community characteristics to the environment. However, convergence studies at the community level often involve only qualitative comparisons of the environment and may fail to identify which environmental variables drive community structure. We tested the hypothesis that the biological traits of fish communities on two continents (Europe and North America) are similarly related to environmental conditions. Specifically, from observations of individual fish made at the microhabitat scale (a few square meters) within French streams, we generated habitat preference models linking traits of fish species to local scale hydraulic conditions (Froude number), Using this information, we then predicted how hydraulics and geomorphology at the larger scale of stream reaches (several pool-riffle sequences) should quantitatively influence the trait composition of fish communities. Trait composition for fishes in stream reaches with low Froude number at low flow or high proportion of pools was predicted as nonbenthic, large, fecund, long-lived, nonstreamlined, and weak swimmers. We tested our predictions in contrasting stream reaches in France (n = 11) and Virginia, USA (n = 76), using analyses of covariance to quantify the relative influence of continent vs. physical habitat variables on fish traits. The reach-scale convergence analysis indicated that trait proportions in the communities differed between continents (up to 55% of the variance in each trait was explained by "continent"), partly due to distinct evolutionary histories. However, within continents, trait proportions were comparably related to the hydraulic and geomorphic variables (up to 54% of the variance within continents explained). In particular, a synthetic measure of fish traits in reaches was well explained (50% of its variance) by the Froude number independently of the continent. The effect of physical variables did not differ across continents for most traits, confirming our predictions qualitatively and quantitatively. Therefore, despite phylogenetic and historical differences between continents, fish communities of France and Virginia exhibit convergence in biological traits related to hydraulics and geomorphology. This convergence reflects morphological and behavioral adaptations to physical stress in streams. This study supports the existence of a habitat template for ecological strategies. Some key quantitative variables that define this habitat template can be identified by characterizing how individual organisms use their physical environment, and by using dimensionless physical variables that reveal common energetic properties in different systems. Overall, quantitative tests of community convergence are efficient tools to demonstrate that some community traits are predictable from environmental features.

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USGS2002

Measuring streamflow in Virginia (2002 revision)

The U.S. Geological Survey (USGS), U.S. Department of the Interior, is the Nation's largest Earth-science information agency. Among its many responsibilities, such as map making and providing information on earthquakes and other natural hazards, the USGS provides information on the Nation's water resources. The USGS has collected and analyzed hydrologic (water-related) information for more than 100 years. In 1889, the first streamflow-gaging station (a site where regular observations of streamflow data are collected) operated in the United States by the USGS was established on the Rio Grande near Embudo, New Mexico. As the need for streamflow data increased, the USGS's streamflow-gaging program has grown to include more than 7,000 continuous-record streamflow-gaging stations. More than 90 percent of these stations are operated with at least partial support from State, local, or other Federal agencies In Virginia, the Department of Environmental Quality (DEQ) is a major cooperator in the streamflow-gaging program, which consists of 161 continuous-record streamflow-gaging stations located throughout the State (fig. 1). The USGS and DEQ cooperate to publish the annual USGS State data report, 'Water Resources Data-Virginia'; this two-volume publication includes streamflow data collected at the 161 streamflow-gaging stations, chemical data collected at 19 streamflow-gaging stations, and ground-water data collected from more than 250 wells located in Virginia.

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USGS2002

Water resources data, Virginia, water year 2001; Volume 1: Surface-water discharge and surface-water quality records

The Water Resources Division of the U.S. Geological Survey, in cooperation with State agencies, obtains a large amount of data pertaining to the water resources of Virginia each water year. These data, accumulated during many water years, constitute a valuable data base for developing an improved understanding of the water resources of the State. To make these data readily available to interested parties outside the Geological Survey, the data are published annually in this report series entitled "Water Resources Data - Virginia."

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USGS2002

Level I water-quality inventory and monitoring, Richmond National Battlefield Park, Virginia

The U.S. Geological Survey conducted a Level I Water-Quality Inventory and Monitoring (WAQIM) data-collection effort for Richmond National Battlefield Park (Richmond NBP) from August 2001 through April 2002. The primary objective of the WAQIM program was to provide the National Park Service (NPS) and Richmond NBP with at least a nominal inventory of its natural resources and to provide those data in a data-management system consistent with park management needs. Water-quality inventory data (physical, chemical, and biological) were collected from "key" water bodies within the boundaries of Richmond NBP. The key water bodies are those waters within park boundaries that are essential to the central cultural, historical or natural resources management themes of the parks or provide habitats to threatened or endangered plants and animals. Data were collected during the fall, winter, spring, and summer over a range of hydrologic conditions. Because of the drought conditions that persisted during the study period, variations in flow between seasons were less pronounced than during normal hydrologic conditions.

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USGS2001

Groundwater residence times in Shenandoah National Park, Blue Ridge Mountains, Virginia, USA: A multi-tracer approach

Chemical and isotopic properties of water discharging from springs and wells in Shenandoah National Park (SNP), near the crest of the Blue Ridge Mountains, VA, USA were monitored to obtain information on groundwater residence times. Investigated time scales included seasonal (wet season, April, 1996; dry season, August–September, 1997), monthly (March through September, 1999) and hourly (30-min interval recording of specific conductance and temperature, March, 1999 through February, 2000). Multiple environmental tracers, including tritium/helium-3 ( 3 H/ 3 He), chlorofluorocarbons (CFCs), sulfur hexafluoride (SF 6 ), sulfur-35 ( 35 S), and stable isotopes ( δ 18 O and δ 2 H) of water, were used to estimate the residence times of shallow groundwater discharging from 34 springs and 15 wells. The most reliable ages of water from springs appear to be based on SF 6 and 3 H/ 3 He, with most ages in the range of 0–3 years. This range is consistent with apparent ages estimated from concentrations of CFCs; however, CFC-based ages have large uncertainties owing to the post-1995 leveling-off of the CFC atmospheric growth curves. Somewhat higher apparent ages are indicated by 35 S (>1.5 years) and seasonal variation of δ 18 O (mean residence time of 5 years) for spring discharge. The higher ages indicated by the 35 S and δ 18 O data reflect travel times through the unsaturated zone and, in the case of 35 S, possible sorption and exchange of S with soils or biomass. In springs sampled in April, 1996, apparent ages derived from the 3 H/ 3 He data (median age of 0.2 years) are lower than those obtained from SF 6 (median age of 4.3 years), and in contrast to median ages from 3 H/ 3 He (0.3 years) and SF 6 (0.7 years) obtained during the late summer dry season of 1997. Monthly samples from 1999 at four springs in SNP had SF 6 apparent ages of only 1.2 to 2.5±0.8 years, and were consistent with the 1997 SF 6 data. Water from springs has low excess air (0–1 cm 3 kg −1 ) and N 2 –Ar temperatures that vary seasonally. Concentrations of He and Ne in excess of solubility equilibrium indicate that the dissolved gases are not fractionated. The seasonal variations in N 2 –Ar temperatures suggest shallow, seasonal recharge, and the excess He and Ne data suggest waters mostly confined to gas exchange in the shallow, mountain-slope, water-table spring systems. Water from wells in the fractured rock contains up to 8 cm 3 kg −1 of excess air with ages in the range of 0–25 years. Transient responses in specific conductance and temperature were observed in spring discharge within several hours of large precipitation events in September, 1999; both parameters increased initially, then decreased to values below pre-storm base-flow values. The groundwater residence times indicate that flushing rates of mobile atmospheric constituents through groundwater to streams draining the higher elevations in SNP average less than 3 years in base-flow conditions.

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USGS2001

Processes controlling the episodic streamwater transport of atrazine and other agrichemicals in an agricultural watershed

Episodic streamwater transport of atrazine (a common agricultural herbicide) and nutrients has been observed throughout agricultural watersheds in the United States and poses a serious threat to the quality of its water resources. Catchment-scale atrazine and nutrient transport processes after agricultural application are still poorly understood, and predicting episodic streamwater composition remains an elusive goal. We instrumented a 1.2-km 2 agricultural catchment near Harrisonburg, Virginia, and examined streamwater, overland flow, soil water, groundwater, and rainfall during the summer of 1998. Storm chemographs demonstrated different patterns for constituents derived primarily from weathering (silica and calcium), compared to constituents derived primarily from early spring land applications (nitrate, atrazine, DOC, potassium, chloride, and sulfate). During storms, the concentrations of silica and calcium decreased, the atrazine response was variable, and the concentrations of nitrate, DOC, potassium, chloride, and sulfate increased; the elevated nitrate signal lagged several hours behind the other elevated constituents. Graphical and statistical analyses indicated a relatively stable spring-fed baseflow was modified by a mixture of overland flow and soil water. A rapid, short-duration overland-flow pulse dominated the streamflow early in the event and contributed most of the potassium, DOC, chloride, suspended sediment, and atrazine. A longer-duration soil–water pulse dominated the streamflow later in the event and contributed the nitrate as well as additional potassium, DOC, sulfate, and atrazine. The contributions to the episodic streamflow were quantified using a flushing model in which overland-flow and soil–water concentrations decreased exponentially with time during an episode. Flushing time constants for the overland-flow and soil–water reservoirs were calculated on a storm-by-storm basis using separate tracers for each time-variable reservoir. Initial component concentrations were estimated through regression analyses. Mass-balance calculations were used for flow separations and to predict the observed streamwater composition. Model forecasts indicated that reduced fertilizer and pesticide application (rather than elimination of overland-flow or soil–water contributions) was necessary to improve the episodic streamwater composition. This study provides important additional understanding of the catchment-scale processes by which land-applied pesticides and nutrients can move through agricultural systems.

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USGS2001

Characteristics of water-well yields in part of the Blue Ridge Geologic Province in Loudoun County, Virginia

Loudoun County, Virginia, which is located about 50 km to the west of Washington, DC, was the site of intensive suburban development during the 1980s and 1990s. In the western half of the county, the source of water for domestic use has been from wells drilled into the fractured crystalline bedrock of the Blue Ridge Geologic Province. A comprehensive digital database that contains information on initial yield, location, depth, elevation, and other data for 3651 wells drilled in this 825.5-km 2 area was combined with a digital geologic map to form the basis for a study of geologic and temporal controls on water-well yields. Statistical modeling procedures were used to determine that mean yields for the wells were significantly different as a function of structural setting, genetic rock type, and geologic map unit. The Bonferroni procedure then was used to determine which paired comparisons contributed to these significant differences. The data were divided into 15 temporal drilling increments to determine if the time-dependent trends that exist for the Loudoun County data are similar to those discovered in a previous study of water-well yields in the Pinardville 7.5-min quadrangle, New Hampshire. In both regions, trends, which include increasing proportions of very low yield wells and increasing well depths through time, and the counterintuitive result of increasing mean well yields through time, were similar. In addition, a yield-to-depth curve similar tothat discovered in the Pinardville quadrangle was recognized in this study. Thus, the temporal model with a feed-forward-loop mechanism to explain the temporal trends in well characteristics proposed for the New Hampshire study appears to apply to western Loudoun County.

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USGS2001

Detection of Perkinsus marinus extracellular proteins tissues of the eastern oyster Crassostrea virginica: Potential use diagnostic assays

Perkinsus marinus , the cause of serious losses of the eastern oyster Crassostrea virginica , secretes extracellular proteins (ECP) in culture (in vitro) including serine proteases. The production of similar ECP in the eastern oyster (in vivo) and their role in pathogenicity, however, remain to be elucidated. The induction and dissemination of these proteins within infected eastern oysters could be critical to understanding the pathologic mechanisms employed as well as to providing the means for rapid and specific diagnosis. To quantify and examine the production of these proteins in the host, polyclonal antibodies were produced against ECP produced during the in vitro culture of P. marinus. By the use of a Western blot technique, these antibodies were shown to recognize a 62-kDa protein antigen expressed in lightly infected oysters, whereas three antigens (62, 38, and 28 kDa) were recognized in heavily infected oysters. Additionally, there was an antigen that is expressed in vitro (40 kDa) that was not detected in vivo and two that were detected in vivo (120 and 32 kDa) but not in vitro. An enzyme-linked immunosorbent assay (ELISA) employing these antibodies indicated that the concentration of secreted antigens is significantly related ( P < 0.05) to the number of cells of P. marinus in infected eastern oyster tissues, as determined by the standard Ray's fluid thioglycollate medium (RFTM) assay. Significant differences ( P < 0.025) between the ELISA and RFTM assays were only observed when oysters possessed less than one hypnospore per gram of tissue. Thus this ELISA proved to be an excellent diagnostic tool, generating values that correlated with the number of protozoal cells present within infected tissues.

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USGS2001

Investigations of the availability and survival of submersed aquatic vegetation propagules in the tidal Potomac River

The establishment of submersed aquatic vegetation (SAV) at unvegetated sites in the freshwater tidal Potomac River was limited primarily by factors other than propagule availability. For two years, traps were used to quantify the amount of plant material reaching three unvegetated sites over the growing season. The calculated flux values provided a gross estimate of the flux of propagules that could potentially survive if other site factors were suitable. The mean flux of Hydrilla verticillata and all other species (≥0.01 gdw m −2 d −1 ) appeared sufficient to favor the establishment of vegetation, particularly considering the high viability (70–100%) of whole plants and fragments under controlled conditions. However, median water clarity values (i.e., for light attenuation, Secchi depth, total suspended solids, and chlorophyll a ) were below SAV restoration goals at all unvegetated sites. Additionally, sediments from unvegetated sites showed a potential for nitrogen limitation of the growth of H. verticillata . Our findings support the hypothesis that in the tidal Potomac River, water clarity and nutrient (especially nitrogen) levels in sediment are key to plant community establishment.

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