Research about North America, Pacific Ocean

USGS2014

Molecular identification of erythrocytic necrosis virus (ENV) from the blood of Pacific herring ( Clupea pallasii )

Viral erythrocytic necrosis (VEN) is a condition affecting the red blood cells of more than 20 species of marine and anadromous fishes in the North Atlantic and North Pacific Oceans. Among populations of Pacific herring ( Clupea pallasii ) on the west coast of North America the disease causes anemia and elevated mortality in periodic epizootics. Presently, VEN is diagnosed by observation of typical cytoplasmic inclusion bodies in stained blood smears from infected fish. The causative agent, erythrocytic necrosis virus (ENV), is unculturable and a presumed iridovirus by electron microscopy. In vivo amplification of the virus in pathogen-free laboratory stocks of Pacific herring with subsequent virus concentration, purification, DNA extraction, and high-throughput sequencing were used to obtain genomic ENV sequences. Fragments with the highest sequence identity to the family Iridoviridae were used to design four sets of ENV-specific polymerase chain reaction (PCR) primers. Testing of blood and tissue samples from experimentally and wild infected Pacific herring as well as DNA extracted from other amphibian and piscine iridoviruses verified the assays were specific to ENV with a limit of detection of 0.0003 ng. Preliminary phylogenetic analyses of a 1448 bp fragment of the putative DNA polymerase gene supported inclusion of ENV in a proposed sixth genus of the family Iridoviridae that contains other erythrocytic viruses from ectothermic hosts. This study provides the first molecular evidence of ENV's inclusion within the Iridoviridae family and offers conventional PCR assays as a means of rapidly surveying the ENV-status of wild and propagated Pacific herring stocks.

Veterinary Microbiology↗

USGS1995

Subduction cycles under western North America during the Mesozoic and Cenozoic eras

An extensive review of geologic and tectonic features of western North America suggests that the interaction of oceanic plates with the continent follows a broad cyclical pattern. In a typical cycle, periods of rapid subduction (7–15 cm/yr), andesitic volcanism, and trench-normal contraction are followed by a shift to trench-normal extension, the onset of voluminous silicic volcanism, formation of large calderas, and the creation of major batholiths. Extension becomes pervasive in metamorphic core complexes, and there is a shift to fundamentally basaltic volcanism, formation of flood basalts, widespread rifting, rotation of terranes, and extensive circulation of fluids throughout the plate margin. Strike-slip faulting becomes widespread with the creation of new tectonostratigraphic terranes. A new subduction zone forms and the cycle repeats. Each cycle is 50–80 m.y. long; cycles since the Triassic have ended and begun at approximately 225, 152, 92, 44, and 15 Ma. The youngest two cycles are diachronous, one from Oregon to Alaska, the other from central Mexico to California. The transitions from one cycle to the next cycle are characterized by rapid and pervasive changes termed, in this chapter, “major chaotic tectonic events.” These events appear to be related to the necking or breaking apart of the formerly subducted slab at shallow depth, the resulting delamination of the plate margin, and the onset of a new subduction cycle. These are times of the most rapid apparent and true polar wander of the North American plate, when the plate appears most free to move relative to surrounding plates and relative to the mantle below the asthenosphere. In western North America, magmatism and tectonics during the Jurassic period are quite similar to magmatism and tectonics since mid-Cretaceous time except strike-slip faulting shifted in sense from left lateral to right lateral.

North America, Pacific Ocean↗