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Research about Illinois, Iowa, Wisconsin

Source-linked reports with geographic coverage including Illinois, Iowa, Wisconsin.

6 recordsLinked to original sources

Environmental DNA metabarcoding as a tool for biodiversity assessment and monitoring: Reconstructing established fish communities of north-temperate lakes and rivers

Aim To evaluate the ability of precipitation-based environmental DNA (eDNA) sample collection and mitochondrial 12S metabarcoding sequencing to reconstruct well-studied fish communities in lakes and rivers. Specific objectives were to 1) determine correlations between eDNA species detections and known community composition based on conventional field sampling, 2) compare efficiency of eDNA to detect fish biodiversity among systems with variable morphologies and trophic states, and 3) determine if species habitat preferences predict eDNA detection. Location Upper Great Lakes Region, North America. Methods Fish community composition was estimated for seven lakes and two Mississippi River navigation pools using sequence data from the mitochondrial 12S gene amplified from 10 to 50 water samples per waterbody collected in 50-mL centrifuge tubes at a single time point. Environmental DNA (eDNA) was concentrated without filtration by centrifuging samples to reduce per-sample handling time. Taxonomic detections from eDNA were compared to established community monitoring databases containing up to 40 years of sampling and a detailed habitat/substrate preference matrix to identify patterns of bias. Results Mitochondrial 12S gene metabarcoding detected 15%–47% of the known species at each waterbody and 30%–76% of known genera. Non-metric multidimensional scaling (NMDS) assessment of the community structure indicated that eDNA-detected communities grouped in a similar pattern as known communities. Discriminant analysis of principal components indicated that there was a high degree of overlap in habitat/substrate preference of eDNA-detected and eDNA-undetected species suggesting limited habitat bias for eDNA sampling. Main conclusions Large numbers of small volume samples sequenced at the mitochondrial 12S gene can describe the coarse community structure of freshwater systems. However, additional conventional sampling and environmental DNA sampling may be necessary for a complete diversity census.

Illinois, Iowa, Wisconsin

Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites

The performance of newly developed New Zealand mudsnail ( Potamopyrgus antipodarum ; NZMS) genetic markers for environmental (eDNA) analysis of water were compared across two laboratories. The genetic markers were tested in four quantitative polymerase chain reaction assays targeting two regions of the NZMS mitochondrial genome, specifically the cytochrome c oxidase subunit 1 (coi) and cytochrome b (cytb) genes. In a blind study, analysts tested each sample eight times with each assay. There were 10 expected-negative samples from the Black River in La Crosse, Wisconsin, 10 expected-positive samples from the Black Earth Creek in Black Earth, Wisconsin, and 10 known-positive samples from the Black River spiked with NZMS DNA. Previously extracted samples, kept at the Upper Midwest Environmental Sciences Center, were pooled by sample location and then equal quantities were distributed between the Upper Midwest Environmental Sciences Center and the Molecular Conservation Genetics Laboratory at the University of Wisconsin-Stevens Point for analysis. The assays tested were (1) the assay targeting cytb with a minor groove binder probe described by Goldberg and others (2013), (2) the cytb assay with a modified double-quenched probe, (3) an assay targeting coi with a double-quenched probe, and (4) a duplex reaction combining the modified cytb assay and the coi assay. Samples were considered positive for the presence of NZMS DNA when quantitative polymerase chain reaction amplification and probe signal was higher than the normalized threshold value above baseline fluorescence. For the duplex assay, samples were considered positive only when both probe signals were higher than the normalized threshold value above baseline fluorescence. Positive results were then confirmed by sequencing the products. All four assays detected the DNA of NZMS in all expected-positive and known-positive samples in both labs. The modified cytb assay, the coi assay, and the duplex assay all failed to detect the DNA of NZMS in all expected-negative samples in both labs. The cytb assay, as described by Goldberg and others (2013), failed to detect the DNA of NZMS in all expected-negative samples for the Molecular Conservation Genetics Laboratory, but some reactions resulted in positive detection in late cycles for 9 of the 10 expected-negative samples at the Upper Midwest Environmental Sciences Center. Amplicons for expected-negative samples with positive reactions were sent for sequencing, and none were confirmed as NZMS. Six amplicons failed to give readable sequences, and three gave sequences without similarity to any known sequence in GenBank. Amplicons from each assay for one representative positive sample were sequenced and identified as NZMS with greater than 99 percent identity. The duplex assay was chosen as the most efficient assay and was used at the Upper Midwest Environmental Sciences Center to analyze triplicate samples from 29 streams in Wisconsin, 8 streams in Illinois, and 8 streams in Iowa. In order to verify results, additional triplicate samples were collected from two of the streams in Iowa and two of the streams in Wisconsin for analysis at the Molecular Conservation Genetics Laboratory. All samples at all sites were negative for NZMS DNA.

Illinois, Iowa, Wisconsin

Lead isotopes from the Upper Mississippi Valley district: A regional perspective

New lead isotopic data on galena from within and peripheral to the Upper Mississippi Valley lead-zinc district make it possible, by extending coverage to outlying locations, to trace the pathway traversed by the mineralizing fluids beyond the boundary of the main district. All but one of the samples exhibit elevated ratios of the radiogenic isotopes typical of the Upper Mississippi Valley ore deposits; 206PbP04Pb ranges from 19.38 to 24.46, 207PbP04Pb ranges from 15.73 to 16.24, and 208PbP04Pb ranges from 39.24 to 43.69. Galena from the Pints quarry near Waterloo, Iowa, has distinctly lower values of these ratios and may not be related paragenetically to the other samples. Otherwise, the lowest ratios are for samples in the southern part of the region in north-central Illinois, and the highest ratios are for samples to the northeast of the main district in the vicinity of Madison, Wisconsin. Thus, an isotopic pattern rather similar to that observed originally by Heyl and others (1966) prevails regionally, although the predominant fluid flow is now believed to have emanated from the Illinois Basin rather than from the Forest City Basin. Metal-bearing brines being driven northward out of the Illinois Basin probably played the key role in mineralization of the Upper Mississippi Valley district. Both the new and the previously reported lead ratios for the Upper Mississippi Valley district are plotted on 207PbP04Pb and Pb208/pb204Pb versus 206PbP04Pb diagrams, which permit their comparison and the calculation of refined slopes for the expanded data set. A two-stage model age for the time of mineralization can be determined from the 207PbP04Pb_Pb206/Pb204 slope, provided that the source age of the lead is known. With our limited know ledge of this source age, the time of mineralization cannot be tightly constrained but is permissive of a Permian or younger lateral secretion event, as suggested by other geochronological results.

Illinois, Iowa, Wisconsin

The 1965 Mississippi River flood in Iowa

The great flood of 1965 on the Mississippi River, along the eastern border if the State, exceeded any flood known in 139 years. It cause damages probably in excess of ten millions of dollars in the State of Iowa. Studied now in progress will more thoroughly cover this and other phases of the flood. The underlying cause of the flood was an abnormally cold winter which prevented the melting of an excessive snow cover in the upper reaches of the basin. Heavy rains late in March followed by rapid melting triggered the runoff which caused the floods. Peak discharges experienced ranged from 276,000 cfs (cubic feet per second) at McGregor near the northern boundary of the State to 327,000 cfs at Keokuk near the southern boundary. Tributary streams in Iowa were receding as the main-stem flood peak passed their mouths. The discharge they contributed was generally insignificant except for the larger streams. Flood data compiled for the part of the River along the eastern border include flood discharges, flood elevations, and the frequency of floods of varying magnitudes. They also include the daily or more frequent stage and discharge data for both the Mississippi River and the downstream gaging stations on Iowa tributaries for the period March-May 1965. Sufficient data are presented to permit studied for preparation of plans for protective works and plans for zoning or for flood plain regulation.

Illinois, Iowa, Wisconsin

The Geology of the Upper Mississippi Valley Zinc-Lead District

The upper Mississippi Valley zinc-lead district includes the southwest part of Wisconsin, the northwest corner of Illinois, and a narrow fringe of Iowa extending from Bellevue to McGregor, just west of the Mississippi River. The total area of the district is 4,000 square miles. The entire district is drained by the Mississippi River and its tributaries. The central part is a rolling plain dissected to a depth of about 300 feet by the larger stream valleys. Marginal parts of the district are relatively hilly. Most of the district lies within the so-called "Driftless Area", but glacial deposits occur in the eastern, southeastern, and western fringes of the district.

Illinois, Iowa, Wisconsin

Zinc and lead deposits of the Upper Mississippi Valley

The zinc and lead mines of the upper Mississippi Valley are in the southwest portion of Wisconsin and in adjacent parts of Illinois mid Iowa. The boundaries of the region are in part indefinite, since sporadic occurrences of the minerals are found outside the mining region proper. It is usual to define the area as including Grant, Lafayette, and Iowa counties in Wisconsin, Jo Daviess County in Illinois, and an irregular narrow belt of territory in Clayton and Dubuque counties paralleling the Mississippi River in Iowa. In this report a slightly smaller area is discussed, the region being limited on the east by the meridian of 90° west longitude. East of this meridian there are a few scattered prospects, hut comparatively little mining has been done in recent years in the area beyond the boundary indicated, so that it is sufficiently accurate for present purposes.

Illinois, Iowa, Wisconsin