Geology topics

Research about Florida

Source-linked reports with geographic coverage including Florida.

At least 379 records · Page 21Linked to original sources

USGS2019

Microbial associations of four species of algal symbiont-bearing Foraminifers from the Florida Reef Tract, USA

While microbiome research is a rapidly expanding field of study, relatively little is known of the microbiomes associated with Foraminifera. This preliminary study investigated microbes associated with four species of Foraminifera, representing two taxonomic orders, which host three kinds of algal endosymbionts. A major objective was to explore potential influences on the microbiome composition, including phylogenetic relatedness among the host species, similarities in algal symbionts hosted, and environmental conditions from which the specimens were collected. Samples examined from two locations along the middle Florida Keys reef tract included 45 foraminiferal specimens and four environmental samples. Bacterial DNA extraction from individual specimens was followed by amplification and amplicon sequencing of the V4 variable region of the 16S rRNA gene; results were obtained from 21 specimens. The Order Miliolida, Family Soritidae, was represented by 5–8 specimens of each of three species: Archaias angulatus and Cyclorbiculina compressa , which both host chlorophyte symbionts, and Sorites orbiculus , which hosts dinoflagellate symbionts. Three Ar. angulatus specimens from which the microbiome was successfully sequenced shared 177 OTUs. Six C. compressa specimens successfully sequenced shared 58 OTUs, of which 31 were also shared by the three specimens of Ar. angulatus . Four successfully sequenced S. orbiculus specimens shared 717 unique OTUs. The 13 soritid specimens shared 26 OTUs, 23 of which represented Proteobacteria, predominantly of the bacterial family Rhodobacteraceae. The fourth foraminiferal species, Amphistegina gibbosa (Order Rotaliida) hosts diatom endosymbionts. Bacterial DNA extraction was attempted on 16 Am. gibbosa , including both normal-appearing and partly-bleached specimens. Only six OTUs, four of which represented Proteobacteria, were found in all eight specimens successfully sequenced. The partly bleached specimens shared nearly twice as many unique microbial OTUs (32) as the normal-appearing specimens (19). All Am. gibbosa specimens shared only four microbial OTUs with the soritid species, three of which may have been contaminants, indicating minimal commonality between the microbiomes of Am. gibbosa and the soritid taxa.

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USGS2019

West Florida shelf pipeline serves as sea turtle benthic habitat based on in-situ towed camera observations

The use of marine offshore benthic habitats by sea turtles is poorly characterized due to the difficulty of obtaining in situ data. Understanding benthic habitat use that is important to the species’ reproduction, foraging, and migrations is critical for guiding management decisions. A towed camera-based assessment survey system (C-BASS) equipped with environmental sensors was used to characterize and assess benthic habitats on the West Florida Shelf (WFS) from 2014 to 2018. During these cruises, sea turtles were opportunistically observed during the surveys, and critical in situ data such as spatiotemporal information, species identification, habitat use, behavior, and environmental data were collected and evaluated. In total, 79 sea turtles were observed during 97 transects of approximately 2700 km of seafloor, which was recorded on 380 h of video. Several sea turtle species were spotted within the WFS, including loggerhead Caretta caretta, Kemp’s ridley Lepidochelys kempii, and green turtles Chelonia mydas. These opportunistic sightings revealed an area of high use on the WFS, an anthropogenic structure known as the Gulfstream natural gas pipeline (GSPL). C-BASS survey results suggest that 2 sea turtle species (C. caretta and L. kempii) utilize this artificial structure primarily as a resting area. We emphasize the importance of combining habitat mapping techniques (towed underwater video and multibeam bathymetry/backscatter) with tracking technology to better understand the fine-scale habitat use of sea turtles.

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USGS2019

Coastal habitat change and marine megafauna behavior: Florida manatees encountering reduced food provisions in a prominent winter refuge

A decline in submerged aquatic vegetation (SAV) within Florida’s spring-fed thermal refuges raises questions about how these systems support winter foraging of Florida manatees Trichechus manatus latirostris . We analyzed telemetry data for 12 manatees over 7 yr to assess their use of Kings Bay, a winter refuge with diminished SAV. After accounting for the effect of water temperature, we hypothesized that the number of trips out of Kings Bay would increase and the time wintering manatees spent in Kings Bay would decrease. Trips out of and into Kings Bay were also compared to assess potential influences on exiting or entering. There were no detectable differences in the number of trips out of the bay or overall time manatees spent in Kings Bay across winters. The percentage of time water temperatures were below 20°C was the single best predictor of increased time spent in Kings Bay. Trips out of Kings Bay were more likely than trips into the bay to occur after 12:00 h and during a high but ebbing tide. Nine manatees tracked for longer than 75 d in winter spent 7 to 57% of their time in the Gulf of Mexico, and 3 of these manatees spent 7 to 65% of the winter >80 km from the mouth of Kings Bay. Results suggest the low amount of SAV in Kings Bay does not obviate its use by manatees, though there are likely tradeoffs for manatees regularly foraging elsewhere. Accounting for movements of Florida manatees through a network of habitats may improve management strategies and facilitate desirable conservation outcomes.

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USGS2019

Enhancing reproductive assessments of the Florida manatee Trichechus manatus latirostris by establishing optimal time period and inhibin B baseline concentrations

The Florida manatee Trichechus manatus latirostris occupies coastal and riverine habitats that may influence the species’ endogenous biological rhythms, including its reproductive potential. Inhibin B provides a biomarker of gonadal function and reproductive potential in humans and other eutherian mammals. This study examined the influence of size, sex, and time of year on inhibin B levels in manatees sampled among 3 habitats with varying degrees of environmental stress in Florida. Inhibin B levels in 38 males averaged (±SE) 4.90 ± 0.23 pg ml -1 ; the average level in 31 females was 5.63 ± 0.46 pg ml -1 . Elevated patterns in inhibin B were exhibited between mid-March and mid-August corresponding to increased mating activity and testicular function, with significant differences in inhibin B levels between male and female manatees (p = 0.03) throughout the year. No significant differences in inhibin B were detected between low- and high-impacted sampling locations during winter, suggesting the potential influence of environmental stress on manatee reproduction may be best examined between mid-March and mid-August—the midpoint of the reproductively active, non-winter time period. Establishing temporal baselines for inhibin B values may be useful in assessing manatee reproductive status and potential conservation threats, shedding light on fertility potential, and enabling future assessment of the effects of stressors on reproduction in Florida manatees.

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USGS2019

Morphology and molecular data reveal invasion of cryptic golden tegus Tupinambis cryptus Murphy et al., 2016) in Florida

Golden Tegus ( Tupinambis teguixin sensu lato ) are native to South America and have established a reproducing population in Miami-Dade County, Florida. Recent work divided the Golden Tegu into four separate species, leaving the specific identity of Golden Tegus in Florida unknown. We used morphometric and mitochondrial data to determine the species identity and likely area of geographic origin for a specimen of Golden Tegu collected in Miami-Dade County, Florida. Our results indicate Cryptic Golden Tegus ( Tupinambis cryptus ) are the species established in Florida. Geographic origin is likely mainland Guyana or Venezuela.

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USGS2019

Eradication of two non-native cichlid fishes in Miami, Florida (USA)

The proliferation of non-native fishes in Florida is a serious problem, and new species continue to be introduced to the state. Fishes in the Family Cichlidae have been especially successful colonizers of south Florida freshwater habitats. Herein we report a multi-agency effort to eradicate two non-native cichlid fishes in Miami, Florida (Bay Snook Petenia splendida and Blue Mbuna Labeotropheus fuelleborni). These fishes were removed before they were observed in the extensive, interconnected canal system through which they may have been able to expand throughout south Florida and access protected areas such as Everglades National Park. The study site, Pinecrest Gardens, is important because it contains remnant coastal cypress-strand habitat in an increasingly urbanized landscape that historically provided refuge to native amphidromous fishes and invertebrates. The project took considerable time (3.5 years), and we detail in this report how it evolved from a focus on isolating the non-native fishes and reducing their population sizes to an eradication. Gardens’ staff hydrologically isolated their ponds from nearby waterbodies by plugging a culvert with a solid gate. That provided the interagency team with more time to remove the potential threats. Compromises were made between fish management strategies and the Gardens’ priorities. Hurricane impacts helped shift priorities to more aggressive fish-management strategies. Cooperation among several federal and state agencies, as well as the Gardens, was key to the project’s success. We hope this effort may serve as a model for removing non-native species before they spread into ecosystems where eradication is not practical.

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USGS2019

Back to the future: Rebuilding the Everglades

Society values landscapes that are engrained in cultural tradition and have a rich connection with human history. As such, there has been a concerted effort to look at the pristine past and develop plans to move the past into the future. However, bringing the past back is constrained by hysteretic changes, irrevocable damages, and anthropogenic trends that do not reflect past conditions. The scale of the Everglades and its importance to water supply and flood control is such that a full recovery, to past, pre-drainage conditions, is not possible. What is possible? The answer is the federally authorized Comprehensive Everglades Restoration Plan (CERP) and the first, most significant implementation of the $12 Billion CERP is the $2 Billion Central Everglades Planning Project (CEPP). CEPP is our “flux-capacitor” in the DeLorean sports car that generates the ability to go back and forth in time, in the movie series “Back to the Future.” The primary hydrological modeling outputs of CEPP came from a version of the Regional Simulation Model (RSM), developed by the South Florida Water Management District. The RSM is the DeLorean vehicle, designed to carry the bags of ecological restoration. Unfortunately, the capacity of this vehicle (i.e., CEPP) is limited, but is it large enough? Will CEPP make a difference? The 20-year RSM simulations (1965 – 1985) without restoration showed nine dry periods when there was no water in the sloughs of Everglades National Park (ENP). When the model was run with CEPP conditions, all of these extreme dry conditions were eliminated. The impact of this was most apparent for fish, especially the size classes that wading birds eat. With our DeLorean (i.e. the RSM) we saw a 60-90% increase in fish density. As one might expect, the birds in our alternative future responded to the fish. The increased volume, flow, and connectivity in the CEPP simulations significantly improved the foraging response of all wading bird species, especially in Water Conservation Area 3 (WCA-3) and ENP. Foraging conditions for an average CEPP year improved by 25-100%. Further downstream, the CEPP simulations showed increased delivery of low nutrient fresh water to the Coastal Everglades and Florida Bay that displaced the relatively P-rich marine water, increased water transparency, and thus decreased algal blooms. However, in a future with accelerating sea levels and estuarine lakes with legacy phosphorus (P), how much more fresh water will be needed to maintain submerged aquatic vegetated habitats? The quest for Everglades Restoration will reach a resource management milestone with the implementation of CEPP. CEPP successfully used a broad suite of hydrological, ecological and societal models to build an acceptable and feasible adaptive management vision of the future. It has been a long and difficult journey, but what we have learned in the process will guide future travelers back in time.

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USGS2019

Non-native marine fishes in Florida: Updated checklist, population status and early detection/rapid response

It has been ten years since the last comprehensive assessment of non-native marine fishes in Florida. Herein, we report sightings of 41 species from Florida coastal waters since the earliest reported sighting in 1984. Information is provided on the population status of each species (e.g., established, eradicated, unknown), and our early detection/rapid response program to remove these fish is described. Of the 41 species, five have established reproducing populations. Nineteen species are either eradicated or failed, while the status of 17 additional species is unknown. Since 1999, 18 individuals comprising 13 species have been removed from coastal waters, most of which were collected through our early-detection/rapid-response program. Many of those fishes were transferred to public aquaria where they were displayed to provide educational information to the public. Most reports of non-native marine fishes originated from citizen scientists, emphasizing the importance of outreach and education.

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USGS2019

Environmental DNA (eDNA) detection of nonnative bullseye snakehead in southern Florida

Bullseye Snakehead Channa marulius (Hamilton 1822) was first detected in the southern Florida town of Tamarac in 2000 and has been expanding its geographic range since. Environmental DNA (eDNA) analysis is a newly-developed technique used to noninvasively detect cryptic or low-density species or those that are logistically difficult-to-study. Genetic material shed into the environment through tissue and body fluids is concentrated from water samples and analyzed for the presence of target species eDNA. To help delineate Bullseye Snakehead’s geographic range, we developed and validated a species-specific eDNA assay for both quantitative and droplet digital PCR (ddPCR). We then used ddPCR to assess 16 locations in southeast Florida using 222 water samples collected from 2015 to 2018. Positive eDNA detections were obtained at all six locations that were within the known geographic range of Bullseye Snakehead. Furthermore, eDNA was detected in six of 10 locations that were previously thought to be outside the periphery of the range but hydrologically connected through the extensive canal system. Over the four years of sampling, estimated occurrence rates (ψ) remained stable and relatively high (ψ = 0.67 [95% credible interval (CI) 0.33–0.95]) near Tamarac, Florida, as compared to the most southern sampling locations (ψ = 0.0–0.37). Bulls- eye Snakehead eDNA estimated occurrence rates in the middle region increased between 2016 (0.28 [95% CI 0.03–0.94]) and 2017 (0.66 [95% CI 0.24–0.98]), potentially reflecting eDNA detections related to a growing or expanding population. Bullseye Snakehead eDNA was detected at low concentrations on the northern and eastern borders of Everglades National Park, which is an important conservation area and UNESCO World Heritage Site. Despite extensive sampling via electrofishing, no Bullseye Snakehead were visually detected in several locations that yielded positive eDNA samples. It is unclear whether eDNA was transported through flowing water or another vector. To date, collection records for this species are confined to urban canals; however, Bullseye Snakehead may use the interconnected system of canals to disperse to natural conservation areas such as Everglades National Park, Big Cypress National Preserve, and Water Conservation Areas, where it may impact native species via predation and competition.

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USGS2019

The Life of P: A biogeochemical and sociopolitical challenge in the Everglades

• Phosphorus (P) is an essential element for all life forms, yet to understand its life cycle and impact we need to grasp not only the biogeochemical life of P, but also how P intersects with human activities and values. • Phosphorus is the limiting nutrient in the oligotrophic Everglades ecosystem. Thus, the anthropogenic addition of P to the landscape and its subsequent transport, transformation, and persistence throughout the Everglades are critical to both the visual appearance and the ecological integrity of the Everglades ecosystem. • Legal and political attention to P in the Everglades have created a powerful social-cultural legacy. This legacy includes the continuing influence of P in Everglades restoration, the dissemination of knowledge about P cycling and mitigation among rural and urban Everglades residents, and social and political realignment of management priorities. • Management decisions based on legal mandates require not only robust scientific data about P-related processes, but also information on the behavior, well-being, and political decisions of tribal nations, agricultural enterprises, anglers, and others who use the South Florida landscape.

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USGS2019

Cultivating future environmental stewards: A case study at John D. MacArthur Beach State Park

Our study supports previous research suggesting that participation in citizen-science programs can significantly enhance student learning and attitudes about science, while simultaneously promoting environmental stewardship. Providing students with the opportunity to collect scientific data through citizen-science programs can increase their understanding of local ecosystems, enhance their observation skills, and can improve their understanding of the scientific process.

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USGS2019

Report of the technical expert workshop: Developing recommendations for field response, captive management, and rehabilitation of sea turtles with fibropapillomatosis

Fibropapillomatosis (FP) is a disease of sea turtles that primarily manifests as tumors of the skin. Strandings of green turtles with this disease have dramatically increased in the Southeast U.S. over the last decade, necessitating a review of various practices related to the capture, handling, and treatment of afflicted turtles. NOAA and USFWS hosted a workshop in St. Petersburg, Florida on September 6, 2017 to seek input on the development of recommendations to address the following key issues regarding green turtles with FP: 1) disposition when encountered under various circumstances (e.g., strandings, research activities, incidental capture, opportunistic observations); 2) rehabilitation and release; 3) response options if rehabilitation capacity is exceeded; and 4) biosecurity measures to prevent anthropogenic spread of the disease. Federal and state resource agency staff and rehabilitation veterinarians were invited to this workshop to provide input for these recommendations. A review of rehabilitation practices and outcome was shared with participants to inform discussions related to rehabilitation and stranding response. Draft recommendations were prepared for the meeting and participants were asked to share their opinions during moderated discussions and via a written feedback instrument. This input was considered and applied to amend the recommendations, which were also peer-reviewed by six additional veterinarians and a stranding biologist with expertise in sea turtles,rehabilitation medicine, and fibropapillomatosis. The intended use for the completed Fibropapillomatosis and Sea Turtles: Recommendations for Field Response, Captive Management, and Rehabilitation is to assist resource agencies with management planning and decisions related to green turtles with FP and to inform relevant aspects of policy, permits, and authorizations under federal and state regulations.

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USGS2018

Using heat as a tracer to determine groundwater seepage in the Indian River Lagoon, Florida, April–November, 2017

The U.S. Geological Survey, in cooperation with the St. Johns River Water Management District, conducted a study to examine water fluxes in two small study areas in the Indian River Lagoon. Vertical arrays of temperature sensors were placed at multiple locations in the lagoon bed to measure temperature time series in the vertical profile. These data at one of the study areas, Eau Gallie, were used in two numerical models, 1DTempPro and VFLUX, to estimate seepage flux rates into the lagoon. 1DTempPro uses an inverse-modeling approach to calibrate groundwater flux to the measured temperature time series. VFLUX isolates the fundamental frequency signal in the temperature data and utilizes the resulting amplitude and phase differences between sensor locations to determine vertical water flux. Field measurements were made during two time periods, March 23 to April 28, 2017, and June 1 to November 3, 2017. Simulating the first, drier period at one location with 1DTempPro helped determine reasonable seepage fluctuations and provided guidelines for choosing which temperature sensor pairs used in the VFLUX simulations would produce the best results. VFLUX simulations at eight locations indicated daily average seepage flux rates of less than 20 centimeters per day (cm/d) and substantial seepage flux out to a distance of at least 110 meters from shore. The spatial variation in average seepage flux rates within 40 meters of shore seemed large, ranging from about 3 to 20 cm/d. In the VFLUX application using the June 1–November 3, 2017 data, the seepage flux has a higher magnitude and fluctuation than the first simulation period, making the isolation of the fundamental temperature frequency signal in the temperature data difficult. However, useful partial or full simulations were achieved at 6 of the 10 locations. The storm surge of Hurricane Irma on September 10, 2017, changed the depths of the sensors relative to the lagoon bed and disrupted the ability of VFLUX to compute seepage flux for the posthurricane period. The June 1 to November 3, 2017, computed seepage flux rates were higher than those for the March 24 to April 28, 2017, period and were sometimes as great as 40 cm/d, and more than 60 cm/d at one location. The seepage time-series data collected during Hurricane Irma indicates a downward seepage flux as a result of the storm surge, followed by upwelling from precipitation recharge inland. The average seepage flux rates are higher than those during the March–April period and are over 25 cm/d near the coast and about 20 cm/d 130 meters offshore.

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USGS2018

Novel ecological and climatic conditions drive rapid adaptation in invasive Florida Burmese pythons

Invasive species provide powerful in situ experimental systems for studying evolution in response to selective pressures in novel habitats. While research has shown that phenotypic evolution can occur rapidly in nature, few examples exist of genome‐wide adaptation on short ‘ecological’ timescales. Burmese pythons ( Python molurus bivittatus ) have become a successful and impactful invasive species in Florida over the last 30 years despite major freeze events that caused high python mortality. We sampled Florida Burmese pythons before and after a major freeze event in 2010 and found evidence for directional selection in genomic regions enriched for genes associated with thermosensation, behavior, and physiology. Several of these genes are linked to regenerative organ growth, an adaptive response that modulates organ size and function with feeding and fasting in pythons. Independent histological and functional genomic datasets provide additional layers of support for a contemporary shift in invasive Burmese python physiology. In the Florida population, a shift towards maintaining an active digestive system may be driven by the fitness benefits of maintaining higher metabolic rates and body temperature during freeze events. Our results suggest that a synergistic interaction between ecological and climatic selection pressures have driven adaptation in Florida Burmese pythons, demonstrating the often‐overlooked potential of rapid adaptation to influence the success of invasive species.

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USGS2018

Three-dimensional seismic characterization of karst in the Floridan aquifer system, southeastern Miami-Dade County, Florida

Two three-dimensional seismic surveys totaling 3.4 square miles were acquired in southeastern Miami-Dade County during 2015 as part of an ongoing broad regional investigation by the U.S. Geological Survey, in cooperation with the Miami-Dade Water and Sewer Department, that includes mapping and karst characterization of the Floridan aquifer system in southeastern Florida. Twenty columniform seismic-sag structures were identified in the three-dimensional seismic data acquired at the South Miami Heights study area in Miami-Dade County, Florida. The seismic-sag structures are commonly composed of a lower and upper seismic facies, where the lower facies is interpreted to be a high permeability column-shaped volume of intense karstification composed of faults, fractures, and collapsed caves, and the upper facies is interpreted to be a lower permeability column-shaped volume composed of sagging suprastratal reflections having little or no faulting, fracturing, or caves. The seismic-sag structures are columniform karst-collapse structures that formed by cave collapse or stoping (upward void migration caused primarily by the mechanical process of ceiling collapse) or both in carbonate rocks of the early Eocene Oldsmar Formation and in some cases in the lowermost part of the middle Eocene Avon Park Formation. Columniform subsidence and sagging of overburden succeeded karst collapse in the lower part of the structures. At the study area, there may be relatively higher potential for the columniform karst-collapse structures to provide cross-formational fluid migration upward from the Boulder Zone into the lower part of middle confining unit 2 as compared to a lower potential for cross-formational fluid migration from the upper part of middle confining unit 2 upward to the Upper Floridan aquifer.

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USGS2018

Hydrodynamics and sediment mobility processes over a degraded senile coral reef

Coral reefs can influence hydrodynamics and morphodynamics by dissipating and refracting incident wave energy, modifying circulation patterns, and altering sediment transport pathways. In this study, the sediment and hydrodynamic response of a senile (dead) barrier reef (Crocker Reef, located in the upper portion of the Florida Reef Tract) to storms and quiescent conditions was evaluated using field observations and the Coupled Ocean-Atmosphere-Wave-Sediment Transport model. Waves, circulation, and resultant sediment mobility were modeled across different reef zones. Sediment mobility during quiescent periods and the passage of far-field storms are driven by nonbreaking waves and, to a lesser degree, regional circulation. Spatial variability in these processes produces the present-day distribution of sediment grain size at Crocker Reef, wherein finer-grain material along a shallow central ridge is frequently mobilized (43% to 62% of the time), winnowed away, and deposited along the lower-energy flanks and in the fore reef where sand mobility occurs less frequently (32% to 43% and 1% to 22% of the time, respectively). Analysis of wave conditions for the period of 2006–2014 supports that wave heights rarely exceed the threshold for breaking (0.1% and 0.3% at the reef crest and at the reef flat, respectively), predominantly during the passage of tropical storms. There is a shift to a wave-breaking regime during near-field storms, creating the potential for mobilization of larger material and enhanced reef degradation. Sediment mobility can be enhanced due to wave skewness or the generation of free infragravity waves during periods of depth-induced wave breaking.

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USGS2018

Continuous stream discharge, salinity, and associated data collected in the lower St. Johns River and its tributaries, Florida, 2016

The U.S. Army Corps of Engineers, Jacksonville District, plans to deepen the St. Johns River channel in Jacksonville, Florida, from 40 to 47 feet along 13 miles of the river channel, beginning at the mouth of the river at the Atlantic Ocean, to accommodate larger, fully loaded cargo vessels. The U.S. Geological Survey installed continuous data-collection stations to monitor discharge, salinity, and associated parameters at 22 sites prior to the commencement of dredging. The U.S. Geological Survey monitored stage and discharge at 13 sites, and water temperature, specific conductance, and salinity at 15 sites; some sites included all parameters. This report contains information pertinent to the data collection sites from their installation date to September 2016, with additional information and data from Hurricane Matthew in October 2016. Site installations began in October 2015; all sites were installed and began collecting data by January 2016. All data available for each site after October 2015 are included in this report. Discharge and salinity ranged widely during the data collection period, which included the effects of Hurricane Hermine in September 2016 and Hurricane Matthew in October 2016. Of the tributaries, annual mean discharge was greatest at Ortega River, followed by Cedar River, Julington Creek, Durbin Creek, and Clapboard Creek. Annual mean salinity for the main-stem sites indicates that salinity decreases with distance upstream, which is expected. The closest tributary site to the Atlantic Ocean (Clapboard Creek) produced the highest annual mean salinity of the tributaries, and Durbin Creek salinity was the lowest of all monitoring locations.

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USGS2018

Cytonuclear discordance in the Florida Everglades invasive Burmese python (Python bivittatus) population reveals possible hybridization with the Indian python (P. molurus)

The invasive Burmese python ( Python bivittatus ) has been reproducing in the Florida Everglades since the 1980s. These giant constrictor snakes have caused a precipitous decline in small mammal populations in southern Florida following escapes or releases from the commercial pet trade. To better understand the invasion pathway and genetic composition of the population, two mitochondrial (mtDNA) loci across 1,398 base pairs were sequenced on 426 snakes and 22 microsatellites were assessed on 389 snakes. Concatenated mtDNA sequences produced six haplotypes with an average nucleotide and haplotype diversity of π = 0.002 and h = 0.097, respectively. Samples collected in Florida from morphologically identified P. bivittatus snakes were similar to published cytochrome oxidase 1 and cytochrome b sequences from both P. bivittatus and Python molurus and were highly divergent (genetic distances of 5.4% and 4.3%, respectively). The average number of microsatellite alleles and expected heterozygosity were N A = 5.50 and H E = 0.60, respectively. Nuclear Bayesian assignment tests supported two genetically distinct groups and an admixed group, not geographically differentiated. The effective population size ( N E = 315.1) was lower than expected for a population this large, but reflected the low genetic diversity overall. The patterns of genetic diversity between mtDNA and microsatellites were disparate, indicating nuclear introgression of separate mtDNA lineages corresponding to cytonuclear discordance. The introgression likely occurred prior to the invasion, but genetic information on the native range and commercial trade is needed for verification. Our finding that the Florida python population is comprised of distinct lineages suggests greater standing variation for adaptation and the potential for broader areas of suitable habitat in the invaded range.

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