USGS ScienceSearch

Geology topics

Teresa J. Newton

Publications and source records attributed to Teresa J. Newton.

At least 19 recordsLinked to original sources

Potential for hydroacoustic technology to describe physical habitat for imperilled native freshwater mussels

The lack of information on what constitutes suitable habitat for native freshwater mussels can limit restoration efforts. While many species reside in silt–sand–gravel substrates, species such as the Spectaclecase ( Cumberlandia monodonta ) and Salamander ( Simpsonaias ambigua ) mussels are thought to be associated with rock structures (e.g., wing dams and rock outcrops) in rivers. Our objective was to assess if hydroacoustic technology could be used to quantify physical habitat features for C. monodonta and S. ambigua . Multibeam echosounder, acoustic Doppler current profiler, sidescan sonar and underwater videography were used to quantify water depth, substrate hardness, bed roughness and bed slope of the riverbed, water velocity, shear velocity and the degree of rock clustering at six sites in the Saint Croix River, Minnesota. The sites varied in type of rock structures and relative abundances of both species. The strength of the associations among physical habitat features and mussel abundance was weak; R 2 values were typically < 0.5. However, species-specific differences in microhabitat were observed. For example, C. monodonta was typically observed at sites with higher velocity and shear velocity compared to S. ambigua . Mussel abundance was greatest at sites that contained crevices of sand surrounded by boulders and bedrock. Future refinements in hydroacoustic methods and post-processing computations could improve predictions. Information on habitat features from occupied and unoccupied sites could help resource managers characterize existing occupied habitats, identify potential reintroduction areas and implement restoration programmes.

Minnesota, Wisconsin

Pit tag application in native freshwater mussels: Case studies across small, medium, and large rivers

Since their first use in the mid-1980s, external passive integrated transponder (PIT) tags have facilitated innovative investigations into multiple biological traits of animals. For native freshwater mussels, PIT tags are frequently used in capture-mark-recapture applications because they allow repeated, noninvasive sampling, are easy to apply, have high retention rates, and have negligible short-term effects on growth and survival. Because of these traits, resource managers and scientists are using PIT-tagged animals to estimate survival and movement of mussels associated with restoration efforts. However, consistency is limited in how PIT tags are affixed, monitored, and reported. Thus, our objectives were to (1) share our collective experiences in PIT tagging mussels across three case studies in small, medium, and large rivers and (2) propose guidelines for tagging and reporting data from PIT tag studies with native freshwater mussels to facilitate comparisons across future studies. The number of studies that have marked mussels with PIT tags has increased over the past 10 years. The ability to detect mussels using PIT tags has substantially advanced research in three areas of mussel ecology: (1) estimating vital rates (e.g., growth and survival), (2) tracking movements and behaviors of captively propagated, wild, and translocated individuals, and (3) improving our understanding of life history traits, such as reproductive timing. Each case study offers insights on tagging methods, tag loss, tag retention, and monitoring frequency across multiple species that range in conservation status from common to rare. We conclude with best-practice guidelines for placing PIT tags on freshwater mussels and a list of variables that could be reported in future studies to facilitate cross-system comparisons.

Freshwater Mollusk Biology and Conservation

Acute toxicity of the lampricide 4-nitro-3-(trifluoromethyl)phenol to the Mussel (Obovaria subrotunda), its host (Percina maculata), and a surrogate mussel species (Obovaria olivaria)

The risk of lampricide applications (such as 4-nitro-3-[trifluoromethyl]phenol [TFM]) to nontarget fauna continues to be a concern within the Great Lakes Fishery Commission Sea Lamprey Control Program, especially among imperiled aquatic species—such as native freshwater mussels. The Grand River (Ohio, USA) is routinely treated for larval sea lampreys ( Petromyzon marinus ), and this river contains populations of the federally threatened mussel Obovaria subrotunda . Given this spatial overlap, information on the sensitivity of O. subrotunda to TFM is needed. Our objectives were to assess the toxicity of TFM to (1) adult Obovaria olivaria (a surrogate for O. subrotunda ), (2) glochidial larvae of O. olivaria and O. subrotunda , (3) juveniles of O. olivaria and O. subrotunda , and (4) adult Percina maculata (host for O. subrotunda glochidia). In acute toxicity tests, TFM was not toxic to glochidia and adult mussels at exposure concentrations that exceed typical treatment rates. Although significant dose–response relationships were observed in hosts and juveniles, survival was ≥95% ( Percina maculata ), ≥93% ( O. olivaria ), and ≥74% ( O. subrotunda ) at typical treatment rates. However, the steep slope of these dose–response relationships indicates that an approximately 20% difference in the treatment level can result in nearly an order of magnitude difference in survival. Collectively, these data indicate that routine sea lamprey control operations are unlikely to acutely affect these species or their host. However, given that many mussel species are long-lived (30–100 years), the risks posed by lampricide treatments in the Great Lakes would be further informed by research on the potential long-term effects of lampricides on imperiled species. Environ Toxicol Chem 2024;00:1–8. Published 2024. This article is a U.S. Government work and is in the public domain in the USA.

Environmental Toxicology & Chemistry

Responses of native freshwater mussels to remediation to remove polychlorinated biphenyl-contaminated sediments in the upper Hudson River

The Hudson River, New York, was contaminated with polychlorinated biphenyls (PCBs) from two manufacturing plants over a period of approximately 30 years, and PCBs are still present in sediment and biota today. The river provides habitat for a variety of plants and animals, including native freshwater mussels. A remediation programme, consisting of dredging followed by the placement of backfilled sediment and/or a subaqueous cap, was initiated in 2009 to remove PCB-contaminated sediments, and managers were concerned about how remediation could affect the mussels. Quantitative surveys were conducted across nine reaches (pools) to estimate species composition, density, population size, population structure, and ecosystem services of mussel assemblages, pre- and post-remediation. Surveys were stratified into remediated areas (targeted for dredging) and non-remediated areas (not targeted for dredging). Pre-remediation, the river contained a low diversity, and high, but variable, density of mussels that were strongly dominated by Elliptio complanata . The presence of few fresh dead mussels indicates low natural mortality. Frequent recruitment by many species, varied length classes, and high biomass also support robust assemblages that perform important ecosystem services, such as biofiltration. Remedial activity removed most of the mussels and the associated services that they perform: their density was reduced by 83% and their filtration capacity was reduced by 93%, relative to non-remediated areas. Areas that were remediated were almost devoid of adult mussels, but the presence of small mussels indicates that natural recolonization is occurring. However, it is unknown whether these assemblages will persist. As mussels are generally long lived and slow growing, natural recovery is anticipated to be slow, in the order of decades.

New York

U.S. Geological Survey science vision for native freshwater mussel research in the United States

Executive Summary North America is a global center for native freshwater mussel (order Unionida, hereinafter “mussels”) diversity, with more than 350 species. Mussels are among the most imperiled fauna on the planet. Reasons for both local and widespread declines in mussels are mostly unknown, although the threats may include habitat loss and fragmentation, diseases, environmental contaminants, altered flow regimes, migration barriers to larval hosts, non-native species, and climate change. Over the past three decades, research on mussels has been substantial. Nevertheless, current conservation and management efforts are limited by significant information gaps. For example, the effects of emerging stressors on mussels are largely unknown and identifying when habitats are rehabilitated and suitable for reestablishment of mussels remains challenging. Additionally, historical and current information on the distribution, taxonomy, and life histories are often unreliable or lacking altogether, and more reliable information is needed for many species. We identified focal research themes, goals, and objectives where research on mussels is needed based on information gaps identified through conversations with resource partners across local, regional, and national organizations. Research on biodiversity seeks to enhance the diversity of mussel species and populations to support healthy aquatic ecosystems. Research on emerging stressors seeks to improve the understanding of how mussel species, populations, and communities respond to emerging stressors, including environmental contaminants and climate change. Research on conservation seeks to enhance the recovery of species and populations and to identify data gaps limiting the conservation of mussels and their habitats. Mussels are in urgent need of proactive conservation because they are an integral part of our natural heritage, enhance biodiversity, and provide vital ecological services that support freshwater ecosystems. The U.S. Geological Survey (USGS) has been, and continues to be, a leader in mussel research. Although the USGS is well suited to address the broad-scale multidisciplinary research needed to conserve mussels, the USGS has had substantial loss of scientists with mussel expertise over the past 20 years. However, the breadth of the USGS expertise on mussels can be leveraged internally across other USGS mission and program areas and externally across research partners. Given the breadth and scope of the issues facing mussels across the United States, the research themes outlined in this science vision can only be accomplished through extensive collaborations between the USGS and the full spectrum of natural resource partners, including other Federal and State agencies, Tribal organizations, universities, industries, and nongovernmental organizations.

Circular

Behavioral and reproductive effects of the lampricides TFM and TFM:1% Niclosamide on native freshwater mussels

The lampricides TFM (3-trifluoromethyl-4′-nitrophenol) and Niclosamide (NIC, 2′, 5-dichloro-4′-nitrosalicylanilide) are used to control sea lamprey populations in the Great Lakes and associated tributaries. Niclosamide is often used as an additive to TFM to reduce the amount of TFM required to control sea lamprey. Concern is growing over the risk that lampricide treatments pose to native freshwater mussels residing in streams. Our objectives were to determine the acute toxicity of TFM and TFM:NIC to free glochidia (removed from the marsupial gills), compare the relative toxicity of TFM and TFM:NIC between free glochidia and brooded glochidia (within the marsupial gills), determine if glochidia age influences toxicity, and assess if exposure of gravid mussels to TFM and TFM:NIC alters behavior and reproduction. Three acute toxicity tests (2:TFM, 1:TFM : NIC) were conducted with glochidia and adults of the plain pocketbook mussel ( Lampsilis cardium ). In tests with glochidia, viability did not differ across TFM and TFM : NIC concentrations that encompassed typical stream treatments. Glochidia age influenced toxicity as glochidia obtained later in the brooding season were less viable than glochidia obtained earlier in the brooding season. Exposure of adults to elevated concentrations of lampricides often resulted in behavioral effects, but rarely affected reproductive endpoints. Because mussels are long-lived (30 to 100 y), even intermittent and short duration exposures may cumulatively affect mussels over their lifetime. The risks posed by lampricide treatments in the Great Lakes would be further informed by research on the sublethal effects of lampricides, particularly effects on non-target organisms such as mussels.

Journal of Great Lakes Research

Assessment of a habitat equivalency analysis for freshwater mussels in the upper Mississippi River

The upper Mississippi River (UMR) contains diverse, dense, and reproducing assemblages of native freshwater mussels. In the case of an injury to mussels and their habitats, such as a hazardous material spill, train derailment, or barge grounding, resource managers have few restoration strategies. Resource managers need a means to document, quantify, and mitigate adverse effects on mussels resulting from injury. Habitat equivalency analysis (HEA), developed for use with a wide variety of habitat types, is a restoration scaling technique that compares ecological services lost from injury to ecological services gained through restoration actions. The U.S. Fish and Wildlife Service and Iowa Department of Natural Resources modified the HEA for use with native mussels. The mussel HEA has been applied within the UMR to estimate the quantity of restoration needed to compensate the public for injuries to mussels due to contaminant spills and construction projects. Our objective was to describe the UMR HEA for a general audience and assess if the four biological input variables used in the mussel HEA were reasonable based on literature values. We also evaluated the performance of HEA under a range of input scenarios. Although the input estimates used in HEA were within ranges reported in the peer-reviewed literature or were supported by professional judgment in the absence of peer-reviewed literature, outcomes of the mussel HEA were highly variable and would benefit from additional research to reduce uncertainty in the biological inputs. The application of HEA to mussels provides resource managers with a tool to quantify mussel-related ecological services lost from injury and to guide restoration efforts in the UMR.

Freshwater Mollusk Biology and Conservation

Population assessment and potential functional roles of native mussels in the Upper Hudson River

General Electric Company (GE) directly and indirectly released polychlorinated biphenyls (PCBs) into the Hudson River and the surrounding environment starting in the late 1940’s, making it one of the most PCB-contaminated rivers in North America. Source control at two GE plant sites was implemented in 2009 to stem the influx of PCBs into the river (NYSDEC 2004; Farrar 2013; NYSDEC 2015). The Hudson River, like many other rivers, contains populations of native freshwater mussels—a group of animals that perform vital functions in freshwater systems. While there was anecdotal evidence of mussels residing in the Upper Hudson River (north of Troy, NY; GE 2005, GE 2009), quantitative data on mussel assemblages was lacking. Systematic, quantitative surveys for native mussels were completed in 2013 and 2015 in a total of six pools of the Upper Hudson River including one reference pool (Feeder Dam) located upstream of the former GE plant sites and five contaminated pools downstream of the GE plant sites (Thompson Island, Fort Miller, Northumberland, Stillwater, and Upper Mechanicville). Surveys were designed to estimate species composition, relative abundance, population size, population structure, and ecological services (i.e., biomass and filtration) of mussel communities prior to and after remedial actions to remove PCB contaminated sediments (i.e., dredged and subsequently capped or backfilled). In most pools, the experimental design incorporated stratification on remediated (before or after remedial activities were completed) and non-remediated areas. ... (access to the full abstract is restricted)

New York

Effects of elevated water temperature on physiological responses in adult freshwater mussels

Freshwater mussels (order Unionoida) face multiple environmental stressors, which pose serious conservation challenges to this diverse assemblage of aquatic invertebrates. Of these stressors, elevated water temperature from global climate change and other anthropogenic sources may be the most ubiquitous and could be placing many mussel populations dangerously close to their thermal maxima. We tested the hypothesis that elevated water temperatures (20, 25, 30 and 35 &deg;C) adversely affected physiological responses in adults of four North American species of mussels ( Amblema plicata , Elliptio complanata, Fusconaia flava and Lampsilis cardium) in 21-d laboratory tests. Oxygen consumption rates were directly affected by temperature in E. complanata and L. cardium , and indirectly affected by temperature in A. plicata and F. flava . Rates of O 2 consumption were generally positively correlated with water temperature. Ammonium excretion rates varied significantly with temperature in E. complanata and generally increased with temperature. The amount of O 2 consumed relative to nitrogen excreted (O : N ratio), varied significantly with temperature in A. plicata , E. complanata and F. flava . The tissue condition index varied among temperatures and species. These data suggest that elevated temperatures can alter metabolic rates in native mussels and may decrease the amount of energy that is available for key biological processes, such as survival, growth and reproduction.

Freshwater Biology

Process, policy, and implementation of pool-wide drawdowns on the Upper Mississippi River: a promising approach for ecological restoration of large impounded rivers

The Upper Mississippi River (UMR) has been developed and subsequently managed for commercial navigation by the U.S. Army Corps of Engineers (USACE). The navigation pools created by a series of lock and dams initially provided a complex of aquatic habitats that supported a variety of fish and wildlife. However, biological productivity declined as the pools aged. The River Resources Forum, an advisory body to the St. Paul District of the USACE, established a multiagency Water Level Management Task Force (WLMTF) to evaluate the potential of water level management to improve ecological function and restore the distribution and abundance of fish and wildlife habitat. The WLMTF identified several water level management options and concluded that summer growing season drawdowns at the pool scale offered the greatest potential to provide habitat benefits over a large area. Here we summarize the process followed to plan and implement pool-wide drawdowns on the UMR, including involvement of stakeholders in decision making, addressing requirements to modify reservoir operating plans, development and evaluation of drawdown alternatives, pool selection, establishment of a monitoring plan, interagency coordination, and a public information campaign. Three pool-wide drawdowns were implemented within the St. Paul District and deemed successful in providing ecological benefits without adversely affecting commercial navigation and recreational use of the pools. Insights are provided based on more than 17&thinsp;years of experience in planning and implementing drawdowns on the UMR.

River Research and Applications

Preliminary characterization of digestive enzymes in freshwater mussels

Resource managers lack an effective chemical tool to control the invasive zebra mussel Dreissena polymorpha . Zebra mussels clog water intakes for hydroelectric companies, harm unionid mussel species, and are believed to be a reservoir of avian botulism. Little is known about the digestive physiology of zebra mussels and unionid mussels. The enzymatic profile of the digestive glands of zebra mussels and native threeridge ( Amblema plicata ) and plain pocketbook mussels ( Lampsilis cardium ) are characterized using a commercial enzyme kit, api ZYM, and validated the kit with reagent-grade enzymes. A linear correlation was shown for only one of nineteen enzymes, tested between the api ZYM kit and a specific enzyme kit. Thus, the api ZYM kit should only be used to make general comparisons of enzyme presence and to observe trends in enzyme activities. Enzymatic trends were seen in the unionid mussel species, but not in zebra mussels sampled 32 days apart from the same location. Enzymatic classes, based on substrate, showed different trends, with proteolytic and phospholytic enzymes having the most change in relative enzyme activity.

Journal of Shellfish Research

Mortality, movement and behaviour of native mussels during a planned water-level drawdown in the Upper Mississippi River

Managers in the Upper Mississippi River (UMR) are using reductions in the River's water levels during summer to mimic historical water regimes and rehabilitate habitats for vegetation and other species. Concerns for the unintended effects of these actions on mussel populations threatened to halt these projects. Our objective was to characterise the survival and movement of two mussel species in the UMR associated with a water level drawdown. During 2009 (no drawdown) and 2010 (0.3 m summer drawdown), we glued passive integrated transponder tags to 10 Amblema plicata and 10 Lampsilis cardium at each of 11 sites. Five sites were in shallow areas expected to be minimally affected by the drawdown (reference sites), and six sites were in shallow areas expected to be directly affected by the drawdown (treatment sites). About equal numbers of sites within both the reference and treatment areas had low and high slopes. Tagged mussels were randomly allocated across sites (within years). Recovery of tagged mussels was >88% in 2009 and 2010. Mortality was similar and low (mean, c . 5% in both species) among reference sites but was variable and relatively high (means, c . 27% in L. cardium and c . 52% in A. plicata ) among treatment sites; variation in mortality among treatment sites appeared related to slope. The study found evidence of drawdown associations with net horizontal movements in A. plicata but not L. cardium . Weekly horizontal movements in both species were significantly correlated with changes in water elevation. We observed significant slope associations related to the drawdown for mortality and net horizontal movement in A. plicata . There were strong species-specific differences in the effects of the drawdown on mortality, vertical movement and horizontal movement. These results suggest that A. plicata responded to the drawdown by vertical movement into the substratum, whereas L. cardium responded by horizontal movement to deeper water. No directionality of movement was observed in either species. Collectively, these data suggest that drawdowns can influence the mortality, movement and behaviour of mussels in the UMR. However, more information on spatial and temporal distributions of mussels is needed to better understand the magnitude of these effects. Results from this study are being used by resource managers to better evaluate the effects of this management tool on native mussel assemblages.

Minnesota, Wisconsin

Sensor data as a measure of native freshwater mussel impact on nitrate formation and food digestion in continuous-flow mesocosms

Native freshwater mussels can influence the aquatic N cycle, but the mechanisms and magnitude of this effect are not fully understood. We assessed the effects of Amblema plicata and Lampsilis cardium on N transformations over 72 d in 4 continuous-flow mesocosms, with 2 replicates of 2 treatments (mesocosms with and without mussels), equipped with electronic water-chemistry sensors. We compared sensor data to discrete sample data to assess the effect of additional sensor measurements on the ability to detect mussel-related effects on NO 3 – formation. Analysis of 624 sensor-based data points detected a nearly 6% increase in NO 3 – concentration in overlying water of mesocosms with mussels relative to mesocosms without mussels (p < 0.05), whereas analysis of 36 discrete samples showed no statistical difference in NO 3 – between treatments. Mussels also significantly increased NO 2 – concentrations in the overlying water, but no significant difference in total N was observed. We used the sensor data for phytoplankton-N and NH 4 + to infer that digestion times in mussels were 13 ± 6 h. The results suggest that rapid increases in phytoplankton-N levels in the overlying water can lead to decreased lag times between phytoplankton-N and NH 4 + maxima. This result indicates that mussels may adjust their digestion rates in response to increased levels of food. The adjustment in digestion time suggests that mussels have a strong response to food availability that can disrupt typical circadian rhythms. Use of sensor data to measure directly and to infer mussel effects on aquatic N transformations at the mesocosm scale could be useful at larger scales in the future.

Freshwater Science

The effects of elevated water temperature on native juvenile mussels: implications for climate change

Native freshwater mussels are a diverse but imperiled fauna and may be especially sensitive to increasing water temperatures because many species already may be living near their upper thermal limits. We tested the hypothesis that elevated water temperatures (20, 25, 30, and 35°C) adversely affected the survival and physiology of 2-mo-old juvenile mussels ( Lampsilis abrupta , Lampsilis siliquoidea , and Megalonaias nervosa ) in 28-d laboratory experiments. The 28-d LT50s (lethal temperature affecting 50% of the population) ranged from 25.3 to 30.3°C across species, and were lowest for L. abrupta and L. siliquoidea . Heart rate of L. siliquoidea was not affected by temperature, but heart rate declined at higher temperatures in L. abrupta and M. nervosa . However, for both of these species, heart rate also declined steadily during the experiment and a strong temperature × time interaction was detected. Juvenile growth was low for all species in all treatments and did not respond directly to temperature, but growth of some species responded to a temperature × time interaction. Responses to thermal stress differed among species, but potential laboratory artifacts may limit applicability of these results to real-world situations. Environmentally relevant estimates of upper thermal tolerances in native mussels are urgently needed to assess the extent of assemblage changes that can be expected in response to global climate change.

Freshwater Science

Water and sediment temperatures at mussel beds in the upper Mississippi River basin

Native freshwater mussels are in global decline and urgently need protection and conservation. Declines in the abundance and diversity of North American mussels have been attributed to human activities that cause pollution, waterquality degradation, and habitat destruction. Recent studies suggest that effects of climate change may also endanger native mussel assemblages, as many mussel species are living close to their upper thermal tolerances. Adult and juvenile mussels spend a large fraction of their lives burrowed into sediments of rivers and lakes. Our objective was to measure surface water and sediment temperatures at known mussel beds in the Upper Mississippi (UMR) and St. Croix (SCR) rivers to estimate the potential for sediments to serve as thermal refugia. Across four mussel beds in the UMR and SCR, surface waters were generally warmer than sediments in summer, and were cooler than sediments in winter. This suggests that sediments may act as a thermal buffer for mussels in these large rivers. Although the magnitude of this effect was usually <3.0°C, sediments were up to 7.5°C cooler at one site in May, suggesting site-specific variation in the ability of sediments to act as thermal buffers. Sediment temperatures in the UMR exceeded those shown to cause mortality in laboratory studies. These data suggest that elevated water temperatures resulting from global warming, thermal discharges, water extraction, and/or droughts have the potential to adversely affect native mussel assemblages.

Upper Mississippi River Basin

Patterns in species richness and assemblage structure of native mussels in the Upper Mississippi River

1. To evaluate patterns in mussel assemblages in the Upper Mississippi River (UMR), data from systematic surveys of mussels conducted in three large reaches (Navigation Pools 5, 6, and 18) from 2005&ndash;2007 were analysed. 2. Nonmetric multi-dimensional scaling analyses and permutation tests indicated that assemblages differed among reaches. The mussel assemblage in Pool 18 was substantially different from the assemblage in Pool 5 and moderately different from the assemblage in Pool 6, whereas assemblages in Pools 5 and 6 were similar. Assemblages in broadly defined, flowing aquatic habitats did not substantially differ. 3. The dissimilarity of Pool 18 was primarily the result of Pool 18 having higher abundances of three Quadrula species (Q. quadrula, Q. pustulosa, and Q. nodulata), and lower abundances of Amblema plicata and Fusconaia flava. 4. Rarefaction analyses showed that species richness and species density were higher in Pool 18 compared with the other two pools. 5. Large-scale patterns in mussel assemblages may be related to other longitudinal trends in the system including geomorphology, water quality, and abundances of fish species that serve as hosts for glochidial larvae. 6. The results suggest that management goals and actions in the UMR may need to account for important differences in mussel assemblages that occur among reaches.

Illinois, Iowa, Minnesota, Wisconsin

Population assessment and potential functional roles of native mussels in the Upper Mississippi River

1. Despite a heightened global concern for native mussels, fundamental research on mussel ecology in large rivers is lacking. These gaps in knowledge about where mussels occur, and why, are limiting habitat restoration activities. 2. Large-scale systematic surveys for native mussels in three reaches of the Upper Mississippi River documented mussel communities composed of 16&ndash;23 species and ranging from 2.9&ndash;4.5 live mussels m -2 that were actively recruiting new cohorts into their populations (87&ndash;100% of the species were found as juveniles 5 years old). Estimates of mean tissue biomass and production in these reaches ranged from 2.1&ndash;3.1 g C m -2 and 0.4&ndash;0.6 g C m -2 year -1 , respectively. 3. Mussels filtered a significant amount of water (range, 0.05&ndash;0.07 m 3 m -2 d -1 ) over a 480 km reach of the Upper Mississippi River &mdash; amounting to a filtration rate of 53.1 million m 3 day -1 . The filtration rate of mussels as a percentage of river discharge ranged from 0.5&ndash;1.4% at high flows (5% exceedance), from 1.5&ndash;4.4% at moderate flows (50% exceedance) and from 4.4&ndash;12.2% during low flows (95% exceedance). 4. Collectively, these data suggest that native mussels play an integral role in this ecosystem by sequestering suspended materials that can be used by other benthic organisms.

Illinois, Iowa, Minnesota, Missouri, Wisconsin

Polymethylene-interrupted fatty acids: Biomarkers for native and exotic mussels in the Laurentian Great Lakes

Freshwater organisms synthesize a wide variety of fatty acids (FAs); however, the ability to synthesize and/or subsequently modify a particular FA is not universal, making it possible to use certain FAs as biomarkers. Herein we document the occurrence of unusual FAs (polymethylene-interrupted fatty acids; PMI-FAs) in select freshwater organisms in the Laurentian Great Lakes. We did not detect PMI-FAs in: (a) natural seston from Lake Erie and Hamilton Harbor (Lake Ontario), (b) various species of laboratory-cultured algae including a green alga ( Scenedesmus obliquus ), two cyanobacteria ( Aphanizomenon flos-aquae and Synechococystis sp.), two diatoms ( Asterionella formosa , Diatoma elongatum ) and a chrysophyte ( Dinobryon cylindricum ) or, (c) zooplankton ( Daphnia spp., calanoid or cyclopoid copepods) from Lake Ontario, suggesting that PMI-FAs are not substantively incorporated into consumers at the phytoplankton&ndash;zooplankton interface. However, these unusual FAs comprised 4-6% of total fatty acids (on a dry tissue weight basis) of native fat mucket ( Lampsilis siliquoidea ) and plain pocketbook ( L. cardium ) mussels and in invasive zebra ( Dreissena polymorpha ) and quagga ( D. bugensis ) mussels. We were able to clearly partition Great Lakes' mussels into three separate groups (zebra, quagga, and native mussels) based solely on their PMI-FA profiles. We also provide evidence for the trophic transfer of PMI-FAs from mussels to various fishes in Lakes Ontario and Michigan, further underlining the potential usefulness of PMI-FAs for tracking the dietary contribution of mollusks in food web and contaminant-fate studies.

Michigan, Ontario, Prince Edward Island, Wisconsin