Shane M. Boylan | USGS Science

USGS2021

Using growth rates to estimate the minimum age and size at sexual maturity in a captive population of the critically endangered Central American river turtle Dermatemys mawii

The Central American river turtle Dermatemys mawii is a critically endangered species that has incurred substantial losses over the last several decades due to overhunting. This species is now being considered for head-starting programs (i.e. captive breeding of turtles for wild release). However, relatively little is known about their life history characteristics, especially with respect to growth and sexual maturation. A robust knowledge of D. mawii life history traits is important in developing conservation management plans. Our research is the first known study to maintain hatchlings, juveniles, and adults in captivity with regular morphometric data collection. We quantified growth rates (cm yr-1) and calculated growth parameters (e.g. growth coefficients) to estimate body size and age at onset of sexual maturity in a group of wild-caught but captive-held and captive-bred D. mawii in Belize. Sizes at the onset of sexual maturity were inferred by segmented linear regressions that identified changes in growth rate by body size. Asymptotic sizes and growth coefficients were calculated using the Fabens method and the Wang method. Parameters from these models were then applied to a modified von Bertalanffy growth equation to estimate age at the onset of sexual maturity. Male and female D. mawii begin sexual maturation at ca. 38.0 cm and 40.0 cm straight-line carapace length, respectively. We estimated ages associated with these sizes at 13.5-16.9 yrs (males) and 13.6-17.3 yrs (females). No previous literature on growth rates or age at maturation for wild or captive D. mawii has been reported, so our results serve as a starting point in conservation management. Given the life history trait of delayed sexual maturity (>10 years), D. mawii may be more sensitive to losses of the adult population. Therefore, the importance of captive breeding and head-starting programs may be concomitant with protecting wild, adult populations.

Toledo↗

USGS2019

Caryospora-like coccidia infecting green turtles (Chelonia mydas): An emerging disease with evidence of interoceanic dissemination

Protozoa morphologically consistent with Caryospora sp. are one of the few pathogens associated with episodic mass mortality events involving free-ranging sea turtles. Parasitism of green turtles (Chelonia mydas) by these coccidia and associated mortality was first reported in maricultured turtles in the Caribbean during the 1970s. Years later, epizootics affecting wild green turtles in Australia occurred in 1991 and 2014. The first clinical cases of Caryospora-like infections reported elsewhere in free-ranging turtles were from the southeastern US in 2012. Following these initial individual cases in this region, we documented an epizootic and mass mortality of green turtles along the Atlantic coast of southern Florida from November 2014 through April 2015 and continued to detect additional, sporadic cases in the southeastern US in subsequent years. No cases of coccidial disease were recorded in the southeastern US prior to 2012 despite clinical evaluation and necropsy of stranded sea turtles in this region since the 1980s, suggesting that the frequency of clinical coccidiosis has increased here. Moreover, we also recorded the first stranding associated with infection by a Caryospora-like organism in Hawai'i in 2018. To further characterize the coccidia, we sequenced part of the 18S ribosomal and mitochondrial cytochrome oxidase I genes of coccidia collected from 62 green turtles found in the southeastern US and from one green turtle found in Hawai'i. We also sequenced the ribosomal internal transcribed spacer regions from selected cases and compared all results with those obtained from Caryospora-like coccidia collected from green turtles found in Australia. Eight distinct genotypes were represented in green turtles from the southeastern US. One genotype predominated and was identical to that of coccidia collected from the green turtle found in Hawai'i. We also found a coccidian genotype in green turtles from Florida and Australia with identical 18S and mitochondrial sequences, and only slight inter-regional differences in the internal transcribed spacer 2. We found no evidence of geographical structuring based on phylogenetic analysis. Low genetic variability among the coccidia found in green turtle populations with minimal natural connectivity suggests recent interoceanic dissemination of these parasites, which could pose a risk to sea turtle populations.

Frontiers in Veterinary Science↗