Natalie C. Hall | USGS Science

USGS2026

Demonstration, validation, and application of hyperspectral microscopy for the collection of cyanobacterial spectral signatures

Cyanobacterial and other algal blooms are an environmental concern in waterbodies worldwide. While these blooms are a nuisance for recreational activities, they can also be harmful to human and wildlife health when the algae produce and release toxins. Algal community composition can be monitored and analyzed by acquiring hyperspectral images that provide information on various photosynthetic and accessory pigments. Validated, traceable measurements are needed to compare data collected by different hyperspectral instruments. In this proof-of-concept study, we detail the development and validation of a custom hyperspectral microscopy imaging system and assess whether this technology can differentiate between cyanobacteria genera based on differences in their reflectance characteristics. As not all cyanobacteria produce toxins, the ability to distinguish among taxa could be used to identify potential toxin-producers and guide field sampling and further research. Spectral characterization of these taxa contributes to remote sensing efforts to characterize and identify cyanobacterial genera at larger spatial scales.

Oregon↗

USGS2021

The presence of denitrifiers in bacterial communities of urban stormwater best management practices (BMPs)

Stormwater best management practices (BMPs) are engineered structures that attempt to mitigate the impacts of stormwater, which can include nitrogen inputs from the surrounding drainage area. The goal of this study was to assess bacterial community composition in different types of stormwater BMP soils to establish whether a particular BMP type harbors more denitrification potential. Soil sampling took place over the summer of 2015 following precipitation events. Soils were sampled from four bioretention facilities, four dry ponds, four surface sand filters, and one dry swale. 16S rRNA gene analysis of extracted DNA and RNA amplicons indicated high bacterial diversity in the soils of all BMP types sampled. An abundance of denitrifiers was also indicated in the extracted DNA using presence/absence of nirS, nirK , and nosZ denitrification genes. BMP soil bacterial communities were impacted by the surrounding soil physiochemistry. Based on the identification of a metabolically-active community of denitrifiers, this study has indicated that denitrification could potentially occur under appropriate conditions in all types of BMP sampled, including surface sand filters that are often viewed as providing low potential for denitrification. The carbon content of incoming stormwater could be providing bacterial communities with denitrification conditions. The findings of this study are especially relevant for land managers in watersheds with legacy nitrogen from former agricultural land use.

Maryland↗