Geology topics

Melody K. Moore

Publications and source records attributed to Melody K. Moore.

3 recordsLinked to original sources

USGS1998

A cryopreservation method for Pasteurella multocida from wetland samples

A cryopreservation method and improved isolation techniques for detection of Pasteurella multocida from wetland samples were developed. Wetland water samples were collected in the field, diluted in dimethyl sulfoxide (DMSO, final concentration 10%), and frozen at -180 C in a liquid nitrogen vapor shipper. Frozen samples were transported to the laboratory where they were subsequently thawed and processed in Pasteurella multocida selective broth (PMSB) to isolate P. multocida. This method allowed for consistent isolation of 2 to 18 organisms/ml from water seeded with known concentrations of P. multocida. The method compared favorably with the standard mouse inoculation method and allowed for preservation of the samples until they could be processed in the laboratory.

California, Nebraska, Nevada↗

USGS1997

Preparation, cryopreservation, and growth of cells prepared from the green turtle (Chelonia mydas)

Techniques are described for preparing, preserving, and growing cell cultures from 30 to 40-day old green turtle embryos (2.0-3.0 cm length) including cells derived from skeletal muscle, liver, heart, kidney, eye, lung, and brain. Acceptable growth of all cells occurred in all standard cell culture media tested, with optimum growth temperature near 30??C. These cell cultures will be used in the study of sea turtle viral diseases including fibropapillomatosis, which is currently epidemic in some green turtle populations.

Methods in Cell Science↗

USGS1997

Investigation into avian mortality in the Playa Lakes region of southeastern New Mexico: Final Report - June 1997

This Final Report is a review of work on a cooperative study undertaken by the USGS Biological Resources Division's National Wildlife Health Center (NWHC) and National Wetlands Research Center (NWRC; formerly the Southern Science Center) from 1994 through 1997. The study was initiated at the request of the Bureau of Land Management (BLM), through a request to the former National Biological Service. The Southeastern New Mexico Playa Lakes Coordinating Committee (SENMPLCC) played an important role in outlining the research needs. The initial Study Plan document, which outlines the background, objectives and methods for the first two years is available as Appendix 1. A letter indicating modifications to the Study Plan was sent to the SENMPLCC chair on April 25,1995, and is Appendix 2. An Interim Report, covering this work was completed and submitted in September 1995. The Literature Review section of the study was completed and presented to SENMPLCC in August, 1995. Following SENMPLCC review, NWHC was asked to develop a series of questions that could be posed from information gained in the initial phase (Appendix 3). The NWHC and NWRC were then directed to begin work to answer the top three questions, within the available fiscal resources. NWRC could continue with work outlined under the original Study Plan (Appendix 1), however an additional Study Plan for experiments performed by NWHC and collaborators and is available as Appendix 4.

New Mexico↗