Geology topics

Marshal S. Hoy

Publications and source records attributed to Marshal S. Hoy.

At least 19 recordsLinked to original sources

USGS2026

Seasonal trophic dynamics drive growth potential and predation risk for reintroduced Chinook salmon in Shasta Reservoir

Reservoir ecosystems can significantly affect anadromous salmon populations reintroduced upstream of impassable high-head dams. In this study, we examine how a novel reservoir food web created by an impoundment can limit juvenile growth or survival through the seasonal production or access to food, promote competition for available resources, and affect risk of the food supply or predation mortality, all of which can be strongly influenced by the thermal regime. Shasta Reservoir, the largest impoundment in California’s Central Valley Project, presents opportunities and risks for winter-run Chinook salmon ( Oncorhynchus tshawytscha ), a federally endangered population targeted for reintroduction into the McCloud River, which flows into the reservoir. We integrated field sampling, stable isotope analysis, hydroacoustic surveys, and bioenergetics modeling to characterize Shasta Reservoir’s food web and evaluate seasonal growth potential and predation risk for juvenile salmon. Zooplankton, dominated by Daphnia , provided favorable foraging conditions in spring but declined sharply by late summer, coinciding with high consumption demand from abundant threadfin shad ( Dorosoma petenense ). Bioenergetics simulations indicated that fry that would ordinarily enter the reservoir in autumn would face poor growth opportunities. They would also be exposed to elevated predation risk driven by warm temperatures and high metabolic demand, particularly from piscivorous salmonids and Sacramento pikeminnow ( Ptychocheilus grandis ). Limited information on predator abundances precludes the ability to quantify total predation demand. Alternatively, juveniles entering the reservoir the following spring would encounter greater prey availability and reduced predation pressure. These findings highlight the strong influence of seasonal thermal structure and food web dynamics on reservoir constraints and underscore the need to incorporate the dynamics of key habitats into reintroduction management and decisions. Our framework provides a quantitative, mechanistically-based approach for evaluating the role of reservoirs in salmon reintroductions above high-head dams.

California↗

USGS2026

Tracking Pacific salmon migrations with a prototype eDNA autosampler

Pacific salmon ( Oncorhynchus spp.) are keystone species that support commercial and recreational fisheries and play a significant role in Indigenous cultures. As a keystone species, they are commonly monitored to assess population metrics and adult and juvenile migration. Environmental DNA (eDNA) has been successfully applied to salmon monitoring, but these efforts have relied on manually collected samples which can limit temporal resolution and increase field effort. Here, we evaluated a prototype autonomous eDNA sampler for fine-scale tracking of adult salmon migrations in the Lake Washington Ship Canal (LWSC) in Washington state, USA. The autosampler was deployed approximately 3 km upstream of the Ballard Locks, where returning adult salmon are counted daily, for 4 months during the adult salmon return in 2022, and it collected eDNA samples twice daily (day and night) onto self-preserving filters. We tested eDNA samples for Chinook Salmon ( Oncorhynchus tshawytscha ), Sockeye Salmon ( O. nerka ) and Coho Salmon ( O. kisutch ) and results were compared with daily adult counts at the Ballard Locks. eDNA detection lagged visual counts by approximately 5 days for Sockeye and Coho salmon and approximately 23 days for Chinook Salmon. The extensive time lag in eDNA detection for Chinook Salmon was likely due to a thermal barrier, delaying their migration through the LWSC. We found no clear effect of day versus night sampling on eDNA detection probability. Two additional experiments were performed: one to assess eDNA degradation on used self-preserving filters stored in the autosampler and another to assess whether our 3 L system flush volume was sufficient to prevent residual eDNA from being carried over from one sample to the next. We found no apparent eDNA degradation but potential for sample-to-sample carry over at this flush volume. Our study highlights the need to consider strategic placement of the autosampler intake to optimize eDNA capture and testing of flush volumes to minimize sample-to-sample carryover. Autonomous eDNA sampling provided efficient, high-frequency, and fine-scale surveillance of salmon migrations and offers a scalable approach for a wide variety of monitoring applications.

Washington↗

USGS2026

Unveiling a legacy of fish introductions to mountain lakes using historical records and eDNA surveys in a National Park

Across the western United States, introductions of non-native fish into historically fishless mountain lakes have impacted native biota. Understanding the impacts of fish introductions is essential for conservation in Olympic National Park, a Biosphere Reserve. We reconstructed fish plantings using records dating back to 1930, followed by environmental DNA (eDNA) surveys to estimate the current distribution of fish and amphibians in 117 remote mountain lakes. We used Bayesian multiscale occupancy models to determine how lake attributes and planting history related to fish and amphibian occupancy. The most frequently detected species were Brook Trout, Rainbow Trout, Cascades Frog, and Northwestern Salamander. eDNA sampling revealed 52 lakes with amphibians only, 45 with fish and amphibians, 14 with fish only, and 6 unoccupied. Of the 53 lakes with planting records, 38 had fish eDNA detected. Fish eDNA was also detected in 21 lakes lacking planting records, which could reflect incomplete records, unauthorized plantings, and false positive detections. Of the three species planted, Cutthroat Trout had the highest failure rate and did not become established in 23 of 28 historically planted lakes. In a subset of 9 lakes sampled for up to 7 years, those with known fish and amphibian presence showed consistent eDNA detections over time. The number of times a lake was stocked was the best predictor of occupancy for Brook and Rainbow trout, while higher occupancy for Brook Trout was also associated with lower elevations, lower solar radiation, and larger lake area. We did not observe widespread negative associations between amphibian occupancy and fish presence, although there was a negative relationship between fish presence and Rough-skinned Newt and Long-toed Salamander occupancy. Cascades Frog occupancy showed no relationship to fish presence or lake traits. Our results suggest mechanisms of fish persistence over time and highlight areas where native amphibians are impacted by introduced fish. These results can guide management options like targeted fish removals that benefit native fauna while still supporting recreational fishing. More broadly, our work demonstrates the value of combining historical records with contemporary surveys and the utility of eDNA for broad-scale surveys of species distribution in remote wilderness areas.

Washington↗

USGS2024

Change in growth and prey utilization for a native salmonid following invasion by an omnivorous minnow in an oligotrophic reservoir

Aquatic invasive species can affect food web structure, native fish growth, and production, depending on the traits of the invasive species and the pre-invasion conditions of the ecosystem. Thermal tolerances and behavioral traits can further influence differential exploitation of resources shared between native and invasive species. An unauthorized introduction of redside shiner ( Richardsonius balteatus ) into reservoirs in the Upper Skagit River, Washington, USA caused concern of potential competition, decreased production, and recruitment of rainbow trout ( Oncorhynchus mykiss ). We combined bioenergetics modeling and stable isotope analysis with field data to quantify consumption demand of native and invasive fishes and related consumption to the availability of key zooplankton prey. Per capita consumption on Daphnia by redside shiner was low; however, their high abundance imposed considerable demand on prey resources in Ross Lake. Although monthly consumption demand by the fish community was less than 50% of the monthly production and biomass of Daphnia in Ross Lake, the current Daphnia densities and growth of rainbow trout were considerably lower than before the invasion. These reductions correspond to lower annual consumption of Daphnia . Our study provides insight on mechanisms that influence food web impacts of an invasive omnivore in cold-water reservoirs.

Washington↗

USGS2024

Shift in piscivory by salmonids following invasion of a minnow in an oligotrophic reservoir

Predation can play an important role in structuring ecological communities, and predator–prey dynamics can be altered following the introduction of new species. An unauthorized introduction of redside shiner ( Richardsonius balteatus ) into reservoirs in the Upper Skagit River, Washington, USA created concern that a consequent shift in predator–prey dynamics in the reservoirs could reduce recruitment and production of native salmonids in the basin. We estimated predation mortality in Ross Lake on nonnative redside shiner and juvenile native salmonids to evaluate the potential role of predation in regulating these populations and limiting survival of native species of concern. We used bioenergetics modelling and stable isotope analysis combined with directed field measurements of growth, seasonal diet and thermal experience of piscivorous salmonids to quantify their consumption demand on prey fishes to evaluate the relative magnitude of predation mortality on invasive redside shiners and native salmonids. While redside shiner are the dominant prey fish species in Ross Lake, the modest biomass of native salmonids consumed could translate into substantial mortality, the magnitude of which depended on the timing and size at which prey fishes were eaten. This information provides important context for how nonnative species may indirectly impact native species through shared predation (apparent competition) and can inform conservation decisions surrounding nonnative species control, sustainability of native salmonids and introductions of anadromous fishes.

Ecology of Freshwater Fish↗

USGS2023

Growth performance of Rainbow Trout in reservoir tributaries and implications for steelhead growth potential above Skagit River dams

Objective In the Pacific Northwest (USA), Pacific salmon Oncorhynchus spp. populations have been declining significantly for decades, prompting stakeholders to respond with a variety of conservation and restoration measures. One such measure being considered in the Skagit River basin (Washington, USA) is the introduction of steelhead Oncorhynchus mykiss (anadromous Rainbow Trout) above the impassable Gorge, Diablo, and Ross dams to bolster their populations. Because freshwater growth is key to survival at subsequent life stages, we evaluated current trends in size and growth of Rainbow Trout among key tributaries to Gorge, Diablo, and Ross reservoirs using empirical data collection and bioenergetics modeling. Methods For nine candidate streams, a bioenergetics model was used to assess how temperature and prey consumption affected growth performance of Rainbow Trout between annuli 1 and 2, and 2 and 3. Thermal scenarios were created to evaluate how fish growth responded to temperature variability while total annual consumption was constrained within empirical growth estimates. We then compared these results to back-calculated size thresholds established by size-at-age observed in wild steelhead adults that returned to the Skagit River below the dams. Result Of the streams proposed for introductions, there was one instance (McMillan Creek) in the nominal simulations where growth met or exceeded the size at annulus 2 or 3 of a returning adult steelhead (24.9 g at annulus 2 and 50.3 g at annulus 3). Modeled growth under different thermal scenarios showed that colder temperatures (0.1–10.7°C, Canyon Creek) produced higher growth than under the nominal or warm scenarios (2.0–15.3°C, Canyon Creek), as well as one additional tributary where size at annulus 2 or 3 (±2 SE) was comparable to the threshold established by adult steelhead below the dams (Big Beaver Creek, annulus 3). Conclusion These results suggest Rainbow Trout growth is most limited by prey availability in the examined upper Skagit tributaries.

Washington↗

USGS2023

Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples

We developed a primer and probe based quantitative PCR assay for use with environmental DNA to detect Northwest salamander ( Ambystoma gracile ), a species endemic to the temperate Pacific coastal region of North America. The assay targets a region in the mitochondrial DNA (mtDNA) D-loop gene. Tests of the assay were performed in silico (using the NCBI BLAST tool), in vitro (using DNA extracted from A. gracile and related species) and in situ (water samples collected from a lake with a known population of A. gracile ). This assay will be useful in efforts to monitor the distribution and occupancy of this species.

Conservation Genetics Resources↗

USGS2021

Growth, survival, and cohort formation of juvenile Lost River (Deltistes luxatus) and shortnose suckers (Chasmistes brevirostris) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California—2019 Monitoring Report

Populations of federally endangered Lost River ( Deltistes luxatus ) and shortnose suckers ( Chasmistes brevirostris ) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir (hereinafter, Clear Lake), California, are experiencing long-term decreases in abundance. Upper Klamath Lake populations are decreasing not only due to adult mortality, which is relatively low, but also because they are not being balanced by recruitment of young adult suckers into known adult spawning aggregations. Long-term monitoring of juvenile sucker populations is conducted to (1) determine if there are annual and species-specific differences in production, survival, and growth, (2) better understand when juvenile sucker mortality is greatest, and (3) help identify potential causes of high juvenile sucker mortality particularly in Upper Klamath Lake. The U.S. Geological Survey monitoring program, that began in 2015, tracks cohorts through summer months and among years in Upper Klamath and Clear Lakes. Data on juvenile suckers captured in trap nets are used to provide information on annual variability in age-0 sucker apparent production, juvenile sucker apparent survival, apparent growth, species composition, and health. Upper Klamath Lake indices of year-class strength indicated that the 2019 year-class was the strongest in the past 5 years of monitoring. Low detections of age-1 and older suckers indicate that the 2018 cohort experienced poor survival within the first year of life. Shortnose suckers constituted the smallest proportion and suckers with uncertain species identification constituted the largest proportion of the 2019 year-class. Small numbers of Lost River sucker were captured consistently throughout the sampling season. The relative abundance of age-0 suckers is not a good indicator of year-class strength in Clear Lake. There were no age-0 suckers captured in Clear Lake during the 2015 and 2019 sampling seasons. Most suckers captured were age-1 Klamath largescale/shortnose suckers, which indicated a relatively strong 2018 cohort. Four-year old juveniles from the 2015 cohort were present in 2019 in Clear Lake. Cohorts that do not recruit to our sampling gear until a year or more of age seem to indicate that (1) a stream resident life history is contributing to the lake population and (2) juvenile suckers occupy the Willow Creek drainage for a full year or more. Although these suckers could be either the non-endangered Klamath largescale or the endangered shortnose suckers, a stream resident life history is consistent with these fish being Klamath largescale suckers. Survival of all distinguishable taxa of juvenile suckers is much higher in Clear Lake than in Upper Klamath Lake, with non-trivial numbers of suckers surviving to join spawning aggregations in most years.

California, Oregon↗

USGS2020

Environmental DNA is an effective tool to track recolonizing migratory fish following large‐scale dam removal

Environmental DNA (eDNA) has emerged as a potentially powerful tool for use in conservation and resource management, including for tracking the recolonization dynamics of fish populations. We used eDNA to assess the effectiveness of dam removal to restore fish passage on the Elwha River in Washington State (USA). Using a suite of 11 species‐specific eDNA polymerase chain reaction (PCR) assays, we showed that most targeted anadromous species (five Pacific Salmon species and Pacific Lamprey) were able to pass upstream of both former dam sites. Multiscale occupancy modeling showed that the timing and spatial extent of recolonization differed among species during the four years of post‐dam removal monitoring. More abundant species like Chinook Salmon and Coho Salmon migrated farther into the upper portions of the watershed than less abundant species like Pink Salmon and Chum Salmon. Sampling also allowed assessment of potamodromous fish species. Bull Trout and Rainbow Trout, ubiquitous species in the watershed, were detected at all sampling locations. Environmental DNA from Brook Trout, a non‐native species isolated between the dams prior to dam removal, was detected downstream of Elwha dam but rarely upstream of the Glines Canyon Dam suggested that the species has not expanded its range appreciably in the watershed following dam removal. We found that eDNA was an effective tool to assess the response of fish populations to large‐scale dam removal on the Elwha River.

Environmental DNA↗

USGS2020

Juvenile Lost River and shortnose sucker year-class formation, survival, and growth in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California—2018 monitoring report

California, Oregon↗

USGS2020

Juvenile Lost River and shortnose sucker year-class formation, survival, and growth in Upper Klamath Lake, Oregon and Clear Lake Reservoir, California—2017 Monitoring Report

Executive Summary Populations of federally endangered Lost River ( Deltistes luxatus ) and shortnose suckers ( Chasmistes brevirostris ) in Upper Klamath Lake, Oregon, and Clear Lake Reservoir (hereinafter referred to as Clear Lake; fig. 1), California, are experiencing long-term declines in abundance. Upper Klamath Lake populations are decreasing because juvenile suckers are not surviving and recruiting into the adult population. Most juvenile sucker mortality occurs within the first year of life in Upper Klamath Lake. Annual production of juvenile suckers in Clear Lake appear to be highly variable and may not occur at all in very dry years. However, juvenile sucker survival is much higher in Clear Lake, with some suckers surviving to join spawning aggregations. Long-term monitoring of juvenile sucker populations is needed to 1) determine if there are annual and species-specific differences in production, survival, and growth; 2) better understand when juvenile sucker mortality is greatest; 3) help identify potential causes of high juvenile sucker mortality particularly in Upper Klamath Lake; and 4) monitor for successful juvenile survival in Upper Klamath Lake. The U.S. Geological Survey (USGS) began a summer juvenile sucker monitoring program in 2015 to track cohorts over time in Upper Klamath and Clear Lakes. The juvenile sucker monitoring program involved using trap net data at fixed sites to determine the status of juvenile suckers. Annual variability in apparent age-0 sucker production, juvenile sucker survival, and growth were tracked. Using genetic markers, suckers were classified as one of three taxa; shortnose (combinations of shortnose and Klamath largescale suckers), Lost River, or suckers with genetic markers of both species (Intermediate [Prob]). By using catch data, we generated taxa-specific indices of year-class strength, August–September apparent survival, and overwinter apparent survival. We also examined the prevalence and severity of afflictions such as parasites, wounds, and deformities. The Upper Klamath Lake year-class strength indices for both Lost River and shortnose suckers were slightly lower in 2015 and 2017 than in 2016. The ratios of age-0 Lost River suckers to age-0 shortnose suckers captured in August in Upper Klamath Lake were low in 2015 and 2017, given that adult Lost River suckers are more abundant and more fecund than adult shortnose suckers. This may indicate lower egg, larval, or juvenile survival or poorer spawning success for Lost River suckers than shortnose suckers in these two years. Apparent relative age-0 survival indices for Lost River suckers from August to September in Upper Klamath Lake were greater in 2015 (0.29) than in 2016 (0.16) or 2017 (0.14). Age-0 shortnose sucker catch rates increased between August and September in 2015, possibly indicating new individuals of this species were still recruiting to the lake between the two sampling periods. August to September relative survival indices for Upper Klamath Lake shortnose suckers were 0.35 in 2016 and 0.00 in 2017. We predicted year-class strength would be greater in Clear Lake in years when high spring-time lake elevations and instream flow allowed adult suckers access to spawning habitat in the Willow Creek drainage. Instream flows and lake elevations were sufficient to allow adult suckers to access Willow Creek during the 2016 and 2017 spawning seasons, and age-0 suckers were detected in Clear Lake both years. Higher lake surface elevations and instream flows in 2017 than in 2016 were not associated with higher year-class strength indices in 2017 than in 2016. Low lake surface elevations appeared to limit access by adults to Willow Creek during the 2014 and 2015 spawning seasons and age-0 suckers were not detected in Clear Lake during these years. Nineteen shortnose suckers from the 2014 cohort were captured in Clear Lake in 2017. A 2015 cohort of shortnose suckers was captured as age-1 in 2016 and as age-2 in 2017. The most likely explanation for increasing catch rates of the 2015 cohort is that the higher Willow Creek flows in 2016 and 2017 facilitated the movement of stream-resident suckers, spawned in 2014 and 2015 downstream into Clear Lake. Due to uncertainty in the genetic identification of non-Lost River suckers, these fish are equally likely to be Klamath largescale or shortnose suckers (Hoy and Ostberg, 2015).

California, Oregon↗

USGS2019

Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America

We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non- Cottus species. We demonstrate the efficacy of these assays by field testing eDNA samples collected from streams inhabited by Cottus . These assays will be particularly useful for detecting Cottus in habitats where one (or more) of several Cottus species could be present.

Pacific Northwest↗

USGS2019

Evaluation of environmental DNA surveys for identifying occupancy and spatial distribution of Pacific Lamprey (Entosphenus tridentatus) and Lampetra spp. in a Washington coast watershed

Surveys of environmental DNA (eDNA) have become an important and multifaceted tool for monitoring and identifying distributions and occupancy of aquatic species. This tool is attractive because it is powerful, easy to apply, and provides an alternative to traditional field survey methods. However, validating eDNA survey methods against traditional field survey methods is warranted prior to their application. We used eDNA and electrofishing to survey 10 sites in 3 tributaries of the Chehalis River, Washington, to infer distribution and occupancy of Entosphenus tridentatus and Lampetra spp. Both methods produced similar detection rates for E . tridentatus , and Lampetra spp. were detected at slightly greater frequency with eDNA in the Black River and Skookumchuck River. Within each of the three tributaries, eDNA concentration was negatively related to sample distance from the Chehalis River mainstem for E . tridentatus but not for Lampetra spp., which indicates E . tridentatus and Lampetra spp. may be distributed differently within tributaries. Application of lamprey eDNA data to a multiscale occupancy model indicated high probability of detecting eDNA in water samples and quantitative PCR (qPCR) assays. Broad distribution and high detection of E. tridentatus and Lampetra spp. suggest robust populations inhabit the Chehalis River basin. Our findings suggest eDNA surveys may be comparable to electrofishing for informing lamprey occupancy and distributions. Such sampling is efficient and cost‐effective and we anticipate that eDNA surveys will become a valuable tool in addressing key research and monitoring needs for conservation and restoration of lampreys in general.

Washington↗

USGS2018

Juvenile Lost River and shortnose sucker year class strength, survival, and growth in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California—2016 Monitoring Report

Executive Summary The largest populations of federally endangered Lost River ( Deltistes luxatus) and shortnose suckers ( Chasmistes brevirostris ) exist in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California. Upper Klamath Lake populations are decreasing because adult mortality, which is relatively low, is not being balanced by recruitment of young adult suckers into known spawning aggregations. Most Upper Klamath Lake juvenile sucker mortality appears to occur within the first year of life. Annual production of juvenile suckers in Clear Lake Reservoir appears to be highly variable and may not occur at all in very dry years. However, juvenile sucker survival is much higher in Clear Lake, with non-trivial numbers of suckers surviving to join spawning aggregations. Long-term monitoring of juvenile sucker populations is needed to (1) determine if there are annual and species-specific differences in production, survival, and growth, (2) to identify the season (summer or winter) in which most mortality occurs, and (3) to help identify potential causes of high juvenile sucker mortality, particularly in Upper Klamath Lake. We initiated an annual juvenile sucker monitoring program in 2015 to track cohorts in 3 months (June, August, and September) annually in Upper Klamath Lake and Clear Lake Reservoir. We tracked annual variability in age-0 sucker apparent production, juvenile sucker apparent survival, and apparent growth. Using genetic markers, we were able to classify suckers as one of three taxa: shortnose or Klamath largescale suckers, Lost River, or suckers with genetic markers of both species (Intermediate Prob[LRS]). Using catch data, we generated taxa-specific indices of year class strength, August–September apparent survival, and overwinter apparent survival. We also examined prevalence and severity of afflictions such as parasites, wounds, and deformities. Indices of year class strength in Upper Klamath Lake were similar for shortnose suckers in 2015 and 2016, but about twice as high for Lost River suckers and suckers having intermediate Prob[LRS] in 2016 than in 2015. Indices of apparent August–September survival were lower in 2016 (0.41) than in 2015 (1.07) for shortnose suckers and suckers identified as having intermediate Prob [LRS] (0.14 in 2016 and 1.69 in 2015). Indices of apparent August—September survival were similar in 2016 (0.16) and 2015 (0.07) for Lost River suckers. Indices of apparent survival were lower for age-0 Lost River suckers than age-0 shortnose suckers in both years. Although samples sizes are small, a declining trend in the ratio of Lost River to shortnose suckers from 28/23 (1.22) as age-0 fish in September of 2015 to 1/9 (0.11) as age-1 fish in June of 2016 is consistent with higher over winter apparent mortality for Lost River suckers than shortnose suckers in Upper Klamath Lake. Shortnose sucker year class strength was greater in years with high Willow Creek inflows and Clear Lake surface elevation during the spawning season, indicating that access to spawning habitat was an important contributing factor. In previous sampling, age-0 sucker catch per unit effort (CPUE) was relatively high in 2011 and 2012, moderately high in 2013, and zero in 2014 and 2015. The 2011 and 2012 year classes continued to be detected, but the 2013 year class went undetected for the first time in 2016. The 2014 year class continued to be undetected in 2016. Three suckers with one annulus each on fin rays were captured in Clear Lake in 2016. Although these fish are potential representatives of the 2015 year class, they were small for their age, indicating they may have hatched in 2016. Age-0 shortnose and Lost River suckers were captured in Clear Lake in 2016, indicating new cohorts of both taxa were produced. Moderate to abundant year classes were produced in 2011, 2012, and 2016 when lake surface elevation greater than 1,378.9 m (4,524 ft) during the February–June spawning season. Also in 2011 and 2016, rapid increases in lake-surface elevation indicated potentially high Willow Creek inflows. A somewhat less abundant year class produced in 2012 than in 2011 and 2016 was associated with lower spawning season inflows. The apparently smaller 2013 year class was formed when Willow Creek inflows were apparently low and lake surface never exceeded 1,379.2 m (4,524.9 ft). In 2014 and 2015, when year-classes were small or not detected, the Clear Lake surface elevations were at or below 1,378.2 m (4,522 ft), and there was very little spring time Willow Creek inflow. Age-0 shortnose sucker CPUE in Clear Lake was correlated with seasonal decreases in water volumes in 2016 and could not be used to create indices of August–September survival. Age-0 shortnose sucker catch rates in Clear Lake Reservoir were about seven times less in August than in September. Meanwhile, the water volume in Clear Lake Reservoir declined by about 36 percent between these two sampling periods. Higher September catch rates may have resulted from additional age-0 suckers entering the lake from the river, a concentrating effect of declining water volumes, or both. Differences in August standard length, apparent growth rates, and the prevalence of abnormalities were consistent with healthier age-0 suckers in Clear Lake Reservoir than in Upper Klamath Lake. Age-0 suckers were larger in August in Clear Lake Reservoir than in Upper Klamath Lake, which may be due to an earlier hatch date, faster growth, or both in Clear Lake Reservoir. Sample sizes were only large enough to compare growth rates of age-0 shortnose suckers from Upper Klamath Lake in 2015 to Clear Lake Reservoir in 2016. Age-0 shortnose suckers grew more between August and September in Clear Lake Reservoir in 2016 than in Upper Klamath Lake in 2015. Petechial hemorrhages of the skin on age-0 suckers were more prevalent in Upper Klamath Lake than in Clear Lake Reservoir in 2016. Deformed opercula, black-spot forming parasites, and infections presumed to be Columnaris sp. were observed on less than 12 percent of suckers from Upper Klamath Lake but were not observed on suckers from Clear Lake Reservoir in 2016.

California, Oregon↗

USGS2018

Concentrations of environmental DNA (eDNA) reflect spawning salmon abundance at fine spatial and temporal scales

Developing fast, cost-effective assessments of wild animal abundance is an important goal for many researchers, and environmental DNA (eDNA) holds much promise for this purpose. However, the quantitative relationship between species abundance and the amount of DNA present in the environment is likely to vary substantially among taxa and with ecological context. Here, we report a strong quantitative relationship between eDNA concentration and the abundance of spawning sockeye salmon in a small stream in Alaska, USA, where we took temporally- and spatially-replicated samples during the spawning period. This high-resolution dataset suggests that (1) eDNA concentrations vary significantly day-to-day, and likely within hours, in the context of the dynamic biological event of a salmon spawning season; (2) eDNA, as detected by species-specific quantitative PCR probes, seems to be conserved over short distances (tens of meters) in running water, but degrade quickly over larger scales (ca. 1.5 km); and (3) factors other than the mere presence of live, individual fish — such as location within the stream, live/dead ratio, and water temperature — can affect the eDNA-biomass correlation in space or time. A multivariate model incorporating both biotic and abiotic variables accounted for over 75% of the eDNA variance observed, suggesting that where a system is well-characterized, it may be possible to predict species' abundance from eDNA surveys, although we underscore that species- and system-specific variables are likely to limit the generality of any given quantitative model. Nevertheless, these findings provide an important step toward quantitative applications of eDNA in conservation and management.

Alaska↗

USGS2017

Health and condition of endangered young-of-the-year Lost River and Shortnose suckers relative to water quality in Upper Klamath Lake, Oregon, 2014–2015

Most mortality of endangered Lost River ( Deltistes luxatus ) and shortnose ( Chasmistes brevirostris ) suckers in Upper Klamath Lake, Oregon, occurs within the first year of life. Juvenile suckers in Clear Lake Reservoir, California, survive longer and may even recruit to the spawning populations. In a previous (2013–2014) study, the health and condition of juvenile suckers and the dynamics of water quality between Upper Klamath Lake and Clear Lake Reservoir were compared. That study found that apparent signs of stress or exposure to irritants, such as peribiliary cuffing in liver tissue and mild inflammation and necrosis in gill tissues, were present in suckers from both lakes and were unlikely to be clues to the cause of differential mortality between lakes. Seasonal trends in energy storage as glycogen and triglycerides were also similar between lakes, indicating prey limitation was not a likely factor in differential mortality. To better understand the relationship between juvenile sucker health and water quality, we examined suckers collected in 2014–2015 from Upper Klamath Lake, where water quality can be dynamic and, at times, extreme. While there were notable differences in water quality and fish health between years, we were not able to identify any specific water-quality-related causes for differential fish condition. Water quality was generally better in 2014 than in 2015. When considered together afflictions and abnormalities generally indicated healthier suckers in 2014 than 2015. Low dissolved-oxygen events (< 4 milligrams per liter) were less frequent and occurred earlier; high pH events (≥ 9.5) were less frequent and shorter in duration; large diel fluctuations in pH (≥ 1.4) were less frequent; water temperatures were warmer, particularly in July and September; and concentrations of microcystin in both large and small fractions of samples were lower in 2014 than in 2015. Total and therefore also un-ionized ammonia were low in 2014–2015 relative to concentrations known to affect suckers. Petechial hemorrhages of the skin, attached Lernaea spp. and eosinophilic hyaline droplets in the kidney tubules were less prevalent in 2014 than in 2015; however, hyperplastic and hypertrophic gill tissue and trichodinids on the gills were observed more frequently in 2014. There were more suckers with normal liver color and texture in 2014 than in 2015. The prevalence of suckers with liver inflammation was greater in 2014 and only observed in suckers collected after August 5, whereas liver inflammation occurred intermittently in 2015. Liver glycogen among suckers decreased in late-August 2014 and increased from early August to mid-September 2015. Lost River suckers had greater whole-body triglyceride content but a larger proportion with an absence of visceral fat observed in 2014 than in 2015. In contrast, shortnose suckers were similar between years in regard to both whole-body triglyceride and visceral fat. Black-spot-forming parasites (trematode metacercariae) were observed in a higher prevalence on shortnose suckers but not Lost River suckers in 2014 than in 2015. Opercular deformities were less prevalent in both species in 2014 than in 2015. Neither gross nor histological examination revealed a high prevalence of abnormalities in suckers that clearly indicate a primary mechanism for juvenile mortality in Upper Klamath Lake. Histological abnormalities were almost always focal and minimal or mild except where associated with parasites. Mild to severe focal abnormalities associated with Lernaea sp. attachment sites and encysted digenean (trematode) metacercariae are unlikely to be associated with mortality. Severe and diffuse inflammation and hyperplasia of the gills associated with Ichthyobodo sp. on one Lost River sucker, may indicate a potential cause of mortality. High mortality may have primarily occurred outside our study period (for example, in spring or over winter), or was caused by a factor that could not be detected with our methods (for example, predation). Alternatively, abnormalities in a small percentage of passively captured suckers in Upper Klamath Lake may indicate health-related issues that were more prevalent in populations than in our samples. Temporary decreases in liver glycogen stores may also indicate periods of stress, which may eventually lead to mortality of young suckers.

Oregon↗

USGS2016

Juvenile sucker cohort tracking data summary and assessment of monitoring program, 2015

Populations of federally endangered Lost River ( Deltistes luxatus ) and shortnose suckers ( Chasmistes brevirostris ) in Upper Klamath Lake, Oregon, are experiencing long-term declines in abundance. Upper Klamath Lake populations are decreasing because adult mortality, which is relatively low, is not being balanced by recruitment of young adult suckers into known adult spawning aggregations. Previous sampling for juvenile suckers indicated that most juvenile sucker mortality in Upper Klamath Lake likely occurs within the first year of life. The importance of juvenile sucker mortality to the dynamics of Clear Lake Reservoir populations is less clear, and factors other than juvenile mortality (such as access to spawning habitat) play a substantial role. For example, production of age-0 juvenile suckers, as determined by fin ray annuli and fin development, has not been detected since 2013 in Clear Lake Reservoir, whereas it is detected annually in Upper Klamath Lake. We initiated a long-term juvenile sucker monitoring program in 2015 designed to track cohorts through seasons and among years in both Upper Klamath Lake and Clear Lake Reservoir. Specifically, our goals are to track annual variability in age-0 sucker production, juvenile sucker survival, growth, and condition. In this first year of the monitoring program, we assessed assumptions that sampled fish were representative of populations of suckers in each lake. The size, age, and species composition of suckers were similar between randomly determined sites and fixed sites in each lake. We captured a wide size and age range of suckers using similar gear, indicating our gear did not exclude older and larger fish. We identified improvements that could be made in the monitoring program including increasing the number of randomly determined sample sites in both lakes, evaluation of gear-size selectivity, and validation of aging methods for juvenile Lost River and shortnose suckers. Differing age composition of juvenile suckers between lakes in our 2015 catches and as reported in previous studies indicate that juvenile suckers are produced in relatively larger numbers each year in Upper Klamath Lake than in Clear Lake Reservoir. Most (96.6 percent) of suckers captured in Upper Klamath Lake in 2015 were age-0, whereas age-0 or age-1 suckers were not captured in Clear Lake Reservoir. Despite ample effort, age-0 suckers have not been captured in Clear Lake Reservoir since 2013. Estimated ages of suckers captured in 2015 in Clear Lake Reservoir ranged from 2 to 6 years. Low flow during spawning seasons in the only known spawning tributary to Clear Lake Reservoir (Willow Creek) appears to explain the lack of age-0 sucker production in recent years. Juvenile sucker mortality is relatively higher in Upper Klamath Lake than in Clear Lake Reservoir. We compared data collected in 2015 to previously published catch rates to produce an index of annual juvenile sucker survival for these species. We calculated indices of annual apparent survival of juvenile sucker ages 0–5 years old in Clear Lake Reservoir to be between 0.37 (±0.86 standard error [SE]) and 0.44 (±0.84 SE). This is the first time indices of annual apparent survival for Lost River and shortnose suckers have been calculated. This estimate has the limitation of being non-species specific because not all individuals were identified to species in previous years, and suckers that were identified included both taxa. In contrast, catch rates decreased by 89 percent for juvenile Lost River suckers and decreased 50 percent for juvenile shortnose suckers in Upper Klamath Lake between August and September 2015. Very low catch rates of age-1 and older suckers in Upper Klamath Lake indicate that annual juvenile sucker survival rates are near zero. Condition of suckers was assessed in 2015 based on age-0 sucker growth rates in Upper Klamath Lake and the prevalence of externally observable afflictions on suckers from both lakes. Age-0 Lost River suckers grew an average (± standard deviation [SD]) of 0.72 (±0.01) millimeters [mm] standard length [SL] per day, and age-0 shortnose suckers grew an average of 0.57 (±0.04) mm SL per day in 2015. This growth rate was similar to growth rates reported for these species in Upper Klamath Lake in previous years. Opercular deformities, skin hemorrhages, black-spot causing parasites, and Lernaea spp. parasitism were the most common afflictions observed on suckers. Observed afflictions were primarily on suckers from Upper Klamath Lake, with the exception of Lernaea spp., which occurred more frequently on suckers from Clear Lake Reservoir. Opercular deformities and black-spot causing parasites were each observed on 5 percent of age-0 suckers from Upper Klamath Lake. Petechial hemorrhaging of the skin was observed on 43 percent of age-0 Lost River suckers, 38 percent of age-0 suckers of undetermined taxa, and only 24 percent of age-0 shortnose suckers from Upper Klamath Lake. Petechial hemorrhaging of the skin was only observed on a single shortnose sucker from Clear Lake Reservoir. Within Upper Klamath Lake, the prevalence of these hemorrhages was exactly twice as high as was reported in 2014.

Oregon↗

USGS2015

Health and condition of endangered juvenile Lost River and shortnose suckers relative to water quality and fish assemblages in Upper Klamath Lake, Oregon, and Clear Lake Reservoir, California

Executive Summary Most mortality of endangered Lost River ( Deltistes luxatus ) and shortnose ( Chasmistes brevirostris ) suckers in Upper Klamath Lake, Oregon, appears to occur within the first year of life. However, juvenile suckers in Clear Lake Reservoir, California, appear to survive longer and may even recruit to the spawning populations. Our goal in this study was to develop productive lines of inquiry into the causes of mortality of juvenile suckers, especially in Upper Klamath Lake, through comparison of sucker health and environmental conditions in both lakes. The health of juvenile suckers was associated with physical, biological, and chemical characteristics in each lake from July to September 2013 and 2014. Water-quality dynamics differed substantially between lakes. Diel fluctuations were greater for dissolved-oxygen concentrations and pH in Upper Klamath Lake than in Clear Lake Reservoir, but diel temperature fluctuations were greater in Clear Lake Reservoir. Minimum dissolved-oxygen concentrations were as low as 1.0 milligram per liter (mg/L) in Upper Klamath Lake, but were no lower than 5.7 mg/L in Clear Lake Reservoir. Unionized ammonia (NH 3 ) concentrations were near or less than the minimum reporting limit of 0.002 mg/L NH 3 in Clear Lake Reservoir and generally were less than the concentration known to cause cellular changes to the gills or mortality of suckers in Upper Klamath Lake. Concentrations of microcystins were less than the detection limit (≤0.10 microgram per liter [μg/L]) in both dissolved (<63 micrometers [μm]) and particulate (≥63 μm) fractions of water samples collected from Clear Lake Reservoir in both years. In Upper Klamath Lake, concentrations of microcystins were relatively low in the 2013 particulate fractions and dissolved fractions of samples in both years, but as high as 44.30 μg/L, more than 44 times the World Health Organization recommended levels for drinking water (1 μg/L), in the 2014 particulate fraction. However, there was little histological evidence and no chemical evidence that these concentrations in water were harmful to suckers that we captured. The species and age compositions of juvenile sucker populations differed between lakes. A total of 98 percent of all juvenile suckers captured in Upper Klamath Lake were age-0. In contrast, as many as six age classes of suckers were represented in Clear Lake Reservoir, indicating much better juvenile survival than in Upper Klamath Lake. Based on genetic species identification, 98 percent of juvenile suckers collected from Clear Lake Reservoir were shortnose or Klamath largescale suckers ( Catostomus snyderi ). In contrast, there was a high degree of apparent genetic hybridization in juvenile suckers collected from Upper Klamath Lake, and both Lost River and non-Lost River juvenile suckers were nearly equally represented in samples. Neither gross nor histological examination revealed a high prevalence of abnormalities in suckers that might indicate a mechanism for juvenile mortality in Upper Klamath Lake. Therefore, high mortality primarily may have occurred outside our study period (for example, in spring or over winter), or was owing to a factor that could not be detected with our methods (for example, predation). Alternatively, abnormalities in a small percentage of passively captured suckers in Upper Klamath Lake may indicate health-related issues that were more prevalent in populations than in our samples. Some apparent symptoms of stress or exposure to irritants, such as peribiliary cuffing in hepatocytes and mild inflammation and necrosis in gill tissues, were present in suckers from both lakes and do not appear to be clues to the cause of differential mortality between lakes. Seasonal trends in energy storage as glycogen and triglycerides were similar between lakes, indicating prey availability was not a factor in differential mortality. Differences in sucker health and condition between lakes were considered the most promising clues to the causes of differential juvenile sucker morality between lakes. A low prevalence of petechial hemorrhaging of the skin (16 percent) and deformed opercula (8 percent) in Upper Klamath Lake suckers may indicate exposure to a toxin other than microcystin. Suckers grew slower in their first year of life, but had similar or greater triglyceride and glycogen levels in Upper Klamath Lake compared to Clear Lake Reservoir. These findings do not suggest a lack of prey quantity but may indicate lower prey quality in Upper Klamath Lake.

California, Oregon↗