Salmonid whirling disease
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Geology topics
Publications and source records attributed to Ken Wolf.
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An isolant of duck plague herpesvirus from the Lake Andes Refuge outbreak was seeded in raw and filter-decontaminated water from two locations on the refuge, held at 4 C, and assayed for infectivity intermittently over a period of 2 mo. From an initial level of about 10 5 PFU per ml, infectivity in the filtered samples uniformly dropped to about 10 4 PFU per ml. Infectivity in the raw samples declined much more rapidly; infectious virus remaining at the end of 2 mo (ca. 10 1 PFU per ml) was only about 0.01% of that originally seeded.
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A plaque assay and some growth characteristics of Egtved virus in a line of rainbow trout cells (RTG-2) are described. Preliminary experiments with metabolic inhibitors suggest that the viral genome is ribonucleic acid.
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No abstract available.