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Geology topics

James Campbell

Publications and source records attributed to James Campbell.

4 recordsLinked to original sources

Autonomous samplers and environmental DNA metabarcoding: Sampling day and primer choice have greatest impact on fish detection probabilities

Unprecedented rates of biodiversity loss and ecosystem function necessitate the use of rapid, efficacious, and cost-effective biomonitoring tools. The combination of autonomous samplers and high throughput sequencing (i.e., “metabarcoding”) of environmental DNA ( eDNA ) samples enables characterization of entire communities at high frequency and can be an important tool for conservation and management, allowing researchers to track fluctuations in biodiversity. We deployed two autonomous samplers at two U.S. Geological Survey streamgage sites in the upper Snake River (Wyoming and Idaho, USA) to collect eDNA samples from July-September 2021 and 2022 to characterize fish diversity. We used a probabilistic approach to evaluate the effects of water temperature, water discharge, filter pore size, water volume filtered, number of samples collected, timing, and primers on the probability of detecting eDNA from fish species known to be present. We detected eDNA from 13/15 species present in these areas of the Snake River. Overall, we did not find evidence that filter pore size, water volume filtered, water discharge, and water temperature affected the probability of detecting fish species’ eDNA . By contrast, primers and sampling day affected fish detection probabilities, indicating that primer choice and sampling day can either over- or under- estimate species diversity. These results indicate that users would ideally consider sampling on non-consecutive days and which primer set will maximize species detections.

Metabarcoding & Metagenomics

Using continuous measurements of turbidity to predict suspended-sediment concentrations, loads, and sources in Flat Creek through the town of Jackson, Wyoming, 2019−20 — A pilot study

Flat Creek, a tributary to the Snake River in northwestern Wyoming, is an important source of irrigation water, fish and wildlife habitat, and local recreation. Since 1996, a section of Flat Creek within the town of Jackson has failed to meet Wyoming Department of Environmental Quality’s surface-water-quality standards for total suspended solids and turbidity required by its State water-use classification. Wyoming Department of Environmental Quality water-quality standards prohibit increases of greater than 10 nephelometric turbidity units (NTU) because of human activities in streambodies of Wyoming. Sediment loading from urban stormwater runoff is hypothesized in previous publications to be the primary cause of impairment, but the relative fine sediment contributions from various sources have not been quantified. In cooperation with the Teton Conservation District, the U.S. Geological Survey began a pilot study in the Flat Creek drainage basin to investigate the use of continuous turbidity measurements to predict suspended-sediment concentrations, loads, and sources through the town of Jackson, Wyoming. The predictions were based on turbidity measurements collected every 15 minutes during parts of water years 2019 and 2020. Analysis of differences in the more than 15,000 turbidity measurements coincident between upstream and downstream streamgages indicated that differences of 10 formazin nephelometric units (FNU) or greater composed about 1 percent of the total accepted measurements during the 2019 and 2020 measurement periods. The median difference in measured turbidity between coincident records at the upstream and downstream streamgages in 2019 was 0.20 FNU and the median difference in 2020 was 0.0 FNU. Calculations of mean total sediment loads in Flat Creek during 2019 and 2020 indicate substantially more suspended-sediment was in Flat Creek below the town of Jackson than above town. Mean total calculated suspended-sediment loads at the upstream streamgage were 26 percent in 2019 and 21 percent in 2020 of the mean total suspended-sediment loads at the downstream streamgage. For measurements occurring at the same time (coincident), mean calculated suspended-sediment loads entering the town of Jackson from Flat Creek were 39 percent in 2019 and 35 percent in 2020 of those loads exiting town in Flat Creek. Incorporating statistical model uncertainty, mean differences between predicted suspended-sediment loads could potentially be zero. The annual period of operations of the South Park Supply Ditch, which diverts water into Flat Creek from the Gros Ventre River, constituted between 91 and 90 percent of the total calculated suspended-sediment load at the upstream streamgage, and between 88 and 87 percent of the loads at the downstream streamgage for coincident periods of record in 2019 and 2020, respectively. However, in the absence of simultaneous continuous monitoring and resulting measurements at the outlet of the South Park Supply Ditch, no robust method was available to quantify suspended-sediment loads from the ditch. A moving average filter was used to identify and isolate short-duration (minutes to hours) spikes in turbidity at the downstream streamgage that were likely caused by overland flow and urban runoff. Suspended-sediment loads during urban runoff constituted about 8 and 10 percent of the total calculated suspended-sediment loads at the downstream streamgage (Flat Creek below Cache Creek, near Jackson, Wyoming; U.S. Geological Survey streamgage 13018350), and 6 and 4 percent of the loads calculated for the record coincident with the upstream streamgage in 2019 and 2020, respectively. Estimated suspended-sediment loads at the upstream streamgage during urban runoff events for the coincident period of record constitute 32 and 40 percent of the total estimated suspended-sediment loads at the downstream streamgage in 2019 and 2020, respectively, indicating sediment loads from urban runoff may contribute less than 10 percent, even as little as 5 percent, of the total sediment load exiting the town of Jackson on Flat Creek. Estimation of the proportion of suspended-sediment loads at the upstream site that originate from the South Park Supply Ditch or Cache Creek can only be done with assumptions but have the potential to be equivalent to or greater than calculated suspended-sediment loads associated with urban runoff.

Wyoming

Comparison of water year 2021 streamflow to historical data at selected sites in the Snake River Basin, Wyoming

The headwaters of the Snake River are in the mountains of northwestern Wyoming on lands primarily administered by the National Park Service and the Bridger-Teton National Forest. Streamflow from the Snake River Basin has been measured at some sites for more than 100 years. Water from this drainage basin is used for recreational, agricultural, and municipal uses and power generation. Because of the many uses of the water and the ongoing drought in the Western United States, there is interest in how streamflow in water year 2021 compared to the historical data. Historical streamflow data are defined as the operational period of the streamgage through water year 2020. A water year is named for the year in which it ends; therefore, water year 2021 is October 1, 2020, through September 30, 2021.

Wyoming

Streamflow gains and losses in New Fork and Green Rivers, upstream from Fontenelle Reservoir, Wyoming, October 2015

The Wyoming Landscape Conservation Initiative is a program created to implement a long-term, science-based program of assessing natural resources while facilitating responsible energy and other development and does studies in much of southwestern Wyoming, including all or parts of Lincoln, Sublette, Fremont, Sweetwater, and Carbon Counties. A synoptic study was completed by the U.S. Geological Survey as part of ongoing contributions to the Wyoming Landscape Conservation Initiative to better understand the streamflow dynamics in the New Fork and Green Rivers in an area with historical, current, and future energy development. Streamflow measurements were collected October 19–22, 2015, at 19 sites on the New Fork and Green Rivers in Wyoming to determine changes in streamflow and, where applicable, describe the inflow and outflow of groundwater. Streamflow in the New Fork River generally increased from 50 cubic feet per second (ft 3 /s) at the most upstream site near Pinedale, Wyoming, to 350 ft 3 /s at the mouth of the river. Streamflow in the Green River generally increased downstream from 250 ft 3 /s at the most upstream site at Warren Bridge to around 800 ft 3 /s at the most downstream site near La Barge, Wyo., upstream from Fontenelle Reservoir. Estimated streamflow gains and losses were calculated for five reaches on the New Fork River. Four of the five reaches on the New Fork River had a change greater than the associated measurement error and were gaining reaches; the reach with the largest gain was the most upstream reach. One reach, the most downstream reach, had a calculated change in streamflow less than the associated measurement error. Estimated streamflow gains and losses were calculated for four reaches on the Green River. One reach was determined to be a gaining reach, one was a losing reach, and two reaches had changes less than the associated measurement error. Comparing the annual streamflow hydrographs for three long-term streamgages likely showed the effects of applying irrigation water to the upstream areas of land draining to the Green and New Fork Rivers. Streamflows in the New Fork River near Big Piney and the Green River near La Barge, Wyo., are sustained later in the season compared to the upstream site of Green River at Warren Bridge, which has few diversions and minimal irrigation.

Wyoming