Geology topics

James A. Luoma

Publications and source records attributed to James A. Luoma.

At least 19 recordsLinked to original sources

USGS2026

Field performance evaluation of a bayluscide 20-percent suspension concentrate formulation

Petromyzon marinus (sea lamprey) is a parasitic, invasive fish of the Laurentian Great Lakes. Since the late 1950s, the Great Lakes Fishery Commission has implemented an integrated Sea Lamprey Control Program (SLCP) that relies on two lampricidal chemicals: 3-(trifluoromethyl)-4-nitrophenol (TFM) and niclosamide. Niclosamide is applied using a bayluscide 20-percent emulsifiable concentrate; however, a solvent in this formulation, N -methyl-2-pyrrolidone, has been linked with worker safety concerns and has contributed to equipment degradation and clogging. To address these limitations, the U.S. Geological Survey, in collaboration with Battelle UK, developed a bayluscide 20-percent suspension concentrate (SC) as a potential alternative formulation. In this study, we evaluated the field performance of SC on the Indian River in Schoolcraft County, Michigan. The objective was to assess the formulation’s compatibility with SLCP application procedures and equipment, and to determine its ability to deliver precise lampricide concentrations in a timely manner. SC was found to dilute easily with stream water and readily combined with TFM. As a result, target lampricide concentrations in the stream were achieved within 1 hour of initiating delivery. Moreover, concentrations remained within 9 percent of target values, with less than 2 percent variation across the width of the stream, demonstrating consistent and uniform distribution. These findings indicate that SC can support accurate and timely lampricide applications. When considered alongside previous research highlighting its favorable selectivity for sea lamprey and improved environmental safety, the results support the pursuit of registration and adoption of SC as a new tool for controlling invasive sea lamprey.

Michigan↗

USGS2025

Evaluation of the acute toxicity of the piscicide TFM to Burbot

Non-target animal sensitivity remains a concern when treating Laurentian Great Lakes streams with 4-nitro-3-(trifluoromethyl)phenol (TFM), the main pesticide used to control Sea Lamprey Petromyzon marinus as part of the bi-national Great Lakes Fishery Commission's Sea Lamprey Control Program. Populations of Burbot Lota lota , a historically and culturally important fish, inhabit some of the streams that are treated with TFM. While many species of fish inhabiting the Great Lakes streams have been assessed for sensitivity to TFM, we are not aware of previous research to assess the risk to Burbot. We assessed the sensitivity of Burbot to TFM using replicate 12-hour flow-through diluter toxicity tests. We found Burbot to have a median lethal concentration (LC 50 ) of 9.74 mg/L, while the minimum lethal concentration (LC 99.9 ) for Sea Lamprey was predicted to be 2.5 mg/L in similar waters. The resulting toxicity ratio (LC 50 of non-target organism/LC 99.9 of Sea Lamprey) of Burbot was 3.90, well above the toxicity ratios for known sensitive species. Our results suggest Burbot are not expected to be adversely affected during a typical TFM stream treatment.

Great Lakes↗

USGS2024

Assessment of the sensitivity of Percina caprodes (logperch) to the pesticide 4-nitro-3-(trifluoromethyl)phenol

A continuous-flow streamside toxicity test was completed to evaluate the risk posed by the use of 4-nitro-3-(trifluoromethyl)phenol (TFM), used to control Petromyzon marinus (sea lamprey), to Percina caprodes (logperch). Logperch are a host fish to the parasitic glochidia life stage of the federally endangered Epioblasma triquetra (snuffbox mussel). Streams with an extant population of snuffbox must be treated before May 1, 2023, to prevent inadvertent take through TFM-related mortality of glochidia-infested fish. Although the concentration of TFM required to induce 99.9 percent mortality of sea lamprey was 6.52 milligrams per liter, the TFM required to induce 25 percent mortality of logperch was 10.14 milligrams per liter. Our data indicate that logperch are not as sensitive to TFM as previously suggested.

Michigan↗

USGS2024

Comparative toxicity of emulsifiable concentrate and suspension concentrate formulations of 2′,5-dichloro-4′-nitrosalicylanilide ethanolamine salt

The 2-aminoethanol salt of niclosamide (2′,5-dichloro-4′-nitrosalicylanilide) is a pesticide known as Bayluscide that is used in conjunction with TFM (4-nitro-3-[trifluoromethyl]phenol), also known as 3-trifluoromethyl-4-nitrophenol) to treat tributaries to the Great Lakes infested with invasive parasitic Petromyzon marinus (sea lamprey). Adding 0.5 to 2 percent Bayluscide with TFM can substantially reduce the amount of TFM required to achieve effective control. Currently, an emulsifiable concentrate (EC) formulation of Bayluscide is used in combination with TFM during some stream treatments completed by the Great Lakes Fishery Commission’s binational sea lamprey control program. The Bayluscide EC formulation is highly effective; however, it degrades application tubing, adheres to application equipment, and raises concerns for worker safety because of the solvent in the formulation, N-methyl-2-pyrrolidone. We collaborated with a pesticide formulation company to develop a Bayluscide 20-percent suspension concentrate (SC) formulation as a potential replacement for the Bayluscide 20-percent EC formulation. The 20-percent SC formulation was specifically developed using inert ingredients approved for use by the U.S. Environmental Protection Agency and the Health Canada Pest Management Regulatory Agency. Although approved for use, the inclusion of a small quantity of an antimicrobial in the formulation warranted evaluating the toxicological profile to sea lamprey and select nontarget fish species. We evaluated and compared the toxicity of the 20-percent SC formulation to the 20-percent EC formulation using continuous-flow diluter systems and larval sea lamprey and select cold-, cool-, and warm-water fish as test animals. Our results demonstrate comparable toxicological profiles between the two formulations with the 20-percent SC formulation being slightly less toxic to the nontarget species evaluated.

Open-File Report↗

USGS2024

Seasonal differences in larval sea lamprey (Petromyzon marinus) sensitivity to the pesticide TFM

Invasive sea lamprey ( Petromyzon marinus ) are controlled in the Great Lakes with 4-nitro-3-(trifluoromethyl)phenol (commonly 3-trifluoromethyl-4-nitrophenol or TFM). The proper amount of TFM must be applied during treatments to effectively kill larval sea lamprey while minimizing impacts to non-target species. In this study, bioassay tests were conducted in May, July, and September in a portable test trailer at six larval sea lamprey infested rivers in Michigan to determine potential seasonal changes in sensitivity of larval sea lamprey to TFM. Larvae greater than 60 mm were collected from each stream and exposed for 12 h in TFM-treated stream water using two independent continuous-flow diluter systems. A suite of water chemistries and larval physiological parameters were collected during the tests and modeled as potential predictors of seasonal changes in the sensitivity of larval sea lamprey to TFM. The observed minimum lethal concentrations to larval sea lamprey were 0–40% lower (May), 8% lower–59% higher (July), and 49–117% higher (September) than sea lamprey control personnel treatment prediction charts. Water temperature, liver glycogen content, and time of year were strongly associated with seasonal differences in TFM sensitivity, offering sea lamprey control personnel more exact predictions to limit potential residual lamprey surviving future treatments.

Michigan↗

USGS2023

Laboratory and field comparisons of TFM bar formulations used to treat small streams for larval sea lamprey

A solid formulation of the pesticide TFM (4-nitro-3-(trifluoromethyl)-phenol) was developed in the 1980s for application in small tributaries during treatments to control invasive sea lamprey (Petromyzon marinus Linnaeus, 1758). Several initial inert ingredients were discontinued and substituted, culminating with an interim formulation that unacceptably softens and rapidly decays in warm conditions. A new TFM bar formulation was developed to resolve poor thermal stability and it was registered with the U.S. Environmental Protection Agency and Health Canada Pesticide Management Regulatory Agency in 2020. Laboratory studies compared the thermostability and dissolution (i.e., TFM release) of the interim and new formulation of TFM bars that were held at 20 C or 45 C for 24 hours prior to evaluation. Field tests compared the dissolution of the interim and new formulation of TFM bars when applied in three small tributaries in Michigan. Laboratory tests show that the new formulation bars remain usable when held at 45 C for 24 hours; whereas, the interim formulation bars partially liquify and are not usable. Field tests indicate the new formulation bars have superior characteristics including a near consistent release of TFM for 1013 hours when applied in waters with a velocity of < 0.06 m/sec. A near consistent release of TFM was observed for a maximum of about 6 hours in one field application of the interim formulation bars. Water temperature and water velocity influenced both formulations; however, the greatest effects were observed with interim formulation bars where higher initial TFM concentrations were followed by precipitous TFM concentration decreases in tributaries with the highest water temperature or velocity. Field treatment applications will provide data for refining application parameters such as the number of bars required per unit discharge at various water temperatures and the acceptable water velocity range for applications.

Michigan↗

USGS2021

Concentration addition and independent action assessments of the binary mixtures of four toxicants on zebra mussel (Dreissena polymorpha) mortality

Researchers most often focus on individual toxicants when identifying effective chemical control agents for aquatic invasive species; however, toxicant mixtures may elicit synergistic effects. Synergistic effects may decrease required concentrations and shorten exposure durations for treatments. We investigated four toxicants (EarthTec QZ, Clam-Trol CT-2, niclosamide, and potassium chloride) that have been considered to control invasive zebra mussels ( Dreissena polymorpha Pallas, 1771). We determined the toxicity of binary mixtures for five different mixture ratios to adult mussels. We compared our observations to predictions made with concentration addition and independent action paradigms, as based on the dose-response relationships of each individual toxicant. We calculated the model deviation ratio for each combination at the LC 50 and LC 90 and identified three possible interactions: synergy, antagonism, and additivity. We found that mixtures of niclosamide and Clam-Trol CT-2 were the most synergistic while mixtures that included potassium chloride were largely additive to antagonistic. The use of synergistic combinations has potential to decrease the overall volume and concentration of individual toxicants required for dreissenid mussel treatments, thereby decreasing cost.

Aquatic Toxicology↗

USGS2021

Avoidance of cold-, cool-, and warm-water fishes to Zequanox® exposure

Zequanox® is a biopesticide registered by the U.S. Environmental Protection Agency (USEPA) and the Canadian Pest Management Regulatory Agency for controlling dreissenid mussels with demonstrated selective toxicity. However, some research has indicated that Zequanox may impact the body condition and survival of some non-target species. We assessed avoidance behaviors of two species of cold-, cool-, and warm-water fishes to Zequanox at the maximum concentration allowed by the USEPA label (100 mg/L as active ingredient). Naïve, juvenile fish (n = 30 per species) were individually observed in a two-flume choice tank through which Zequanox-treated and untreated water simultaneously flowed in an unobstructed arena. Individual fish were observed during an untreated control period (20 min) and two Zequanox-exposure periods (20 min each). Treatment was alternated between arena sides to account for potential side bias in the test subjects. Positional data were collected and tabulated in real time with EthoVision® XT software. Zequanox concentrations and water quality properties (pH, dissolved oxygen, temperature, and specific conductance) were monitored during each trial. Analysis of treatment response was performed using a contrast within linear mixed-effects models. Our results indicate that Brook Trout, Lake Trout, and Bluegill avoided Zequanox-treated water, Yellow Perch were indifferent to Zequanox-treated water, and Lake Sturgeon and Fathead Minnow were attracted to Zequanox-treated water. These results combined with existing species sensitivity literature may help inform resource managers of potential treatment-related risks.

Management of Biological Invasions↗

USGS2021

Field evaluation of an improved solid TFM formulation for use in treating small tributary streams

A solid lampricide formulation containing 23% 3-trifluoromethyl-4-nitrophenol (TFM) as the active ingredient was developed in the mid-1980s for use in small tributaries of dendritic streams during routine treatments to kill larval sea lamprey. This TFM bar formulation was designed to use a matrix of commercially prepared surfactants that would dissolve and slowly release their TFM payload over an 8–10-hour period. Although this formulation has proven useful, several matrix surfactants have been discontinued, resulting in the need to reformulate the TFM bar multiple times. Maintaining acceptable performance of the TFM bars while reformulating has been challenging. As a result, an experimental surfactant-free tableted TFM formulation was developed as a potential TFM bar replacement. Release of TFM from the tablet formulation was evaluated in four independent experimental applications made over varied substrates in three small tributaries of the Ford River (Delta County, Michigan). For each tributary, TFM release from tablets was modeled using exponential decay curves and the time required to release 25, 50, 75 and 90% of the TFM tablets was calculated. Differences in water-quality properties were detected using one-way analysis of variance tests, and post-hoc Tukey Honest Significant Difference tests were used to determine which water-quality properties differed among the trials. The influences of water temperature and water velocity on the release of TFM from the tablets has been previously reported; however, in this study substrate type also appeared to be an indicator of TFM release. In this study the performance of the TFM tablets appeared acceptable; however, it may be beneficial to conduct additional investigations to determine storage stability and handling durability as well as to identify potential challenges with mass production.

Report↗

USGS2020

Development and evaluation of an improved TFM formulation for use in feeder stream treatments

The binational Great Lakes Fishery Commission sponsored Sea Lamprey Control Program effectively utilizes a variety of lampricide tools to keep populations of parasitic sea lampreys in the Great Lakes at levels that do not cause undue economic or ecological damage. The most widely used toxicant used in lampricide formulations is 3-trifluoromethyl-4-nitrophenol (TFM). In typical treatments, a liquid TFM formulation is applied to lamprey producing streams continuously for 10–14 hours to produce a moving block of lampricide-treated water that kills larval lamprey before they metamorphose into their parasitic lifestage. In many smaller tributaries of dendritic streams a solid bar formulation of TFM is used to supplement the mainstem treatment block. These supplemental TFM bar applications are coordinated with the arrival of the mainstem lampricide to prevent larval sea lamprey from seeking refuge in untreated waters and surviving the treatment. TFM bars are produced from formulated surfactants and designed to release TFM over an 8–10-hour period, depending on water temperature and velocity. However, some of the surfactants have been discontinued resulting in the reformulation of the TFM bars multiple times. As a result of these reformulations, TFM bar performance has declined. An experimental surfactant-free solid TFM tablet formulation was developed as a potential replacement for TFM bars. Release of TFM from the experimental tablets was evaluated using replicated laboratory dissolution trials conducted at three water temperatures and three water velocities. A continuous-flow laboratory flume was used for the dissolution trials and the decay of the tablets was modeled using logistic decay curves. Time required for the TFM tablet to decay 50 and 99% were compared among the groups using a two-way analysis of variance. Post-hoc Tukey Honest Significant Difference tests indicated that both water temperature and water velocity influenced the decay of the tablet; however, neither water temperature or water velocity appeared to dramatically influence TFM release. Results from this laboratory study indicate that the next stage of evaluating the TFM tablets using field tests is warranted.

Project Completion Report↗

USGS2019

Assessment of uncontained Zequanox applications for zebra mussel control in a Midwestern lake

Zebra mussels ( Dreissena polymorpha ) are invasive bivalves that have perturbed aquatic ecosystems within North America since their introduction in the mid-1980s. Control of zebra mussels has largely been restricted to raw water conveyance systems and associated infrastructures because few control products are registered for application in surface waters. The biopesticide Zequanox was registered in 2014 by the U.S. Environmental Protection Agency for controlling dreissenid mussels (zebra and quagga mussels ( Dreissena rostriformis bugensis ) in surface waters. Previous Zequanox applications in surface waters have used vertical impermeable-membrane barriers to contain treated water. Studies have indicated that uncontained applications may be successful if Zequanox suspensions of the correct viscosity are applied to facilitate the creation of stratified benthic treatment layer. In this study, Zequanox was applied to replicate 0.30-hectare plots within a small inland lake using a custom-engineered, boat-mounted application system to determine if uncontained Zequanox applications could be used to manage zebra mussel populations and to protect native unionid mussels within zebra mussel infested waters. To determine success, the following specific objectives were investigated during, 30 days after, and/or 1 year after Zequanox exposure: (1) evaluate Zequanox concentrations during exposure; (2) monitor water quality during and after exposure; (3) evaluate the mortality of zebra mussels that were caged within treatment zones during the exposures; (4) evaluate the densities of naturally occurring zebra mussels with treatment zones before and after Zequanox exposure; and (5) evaluate the survival, condition, and dreissenid infestation of native mussels in the treatment zones before and after Zequanox exposure. Zequanox rapidly dissipated from the treated plots, resulting in no appreciable treatment-related mortality of zebra mussels and insignificant impacts to water quality. Zequanox exposure-related impacts to native mussels were not observed.

Michigan↗

USGS2019

Optimum electrofishing waveforms and parameters to induce a capture-prone response in juvenile Grass Carp

Grass Carp (Ctenopharyngodon idella) are a non-native species to North America that were first introduced for vegetation control in the 1960s. However, wild-reproducing Grass Carp can negatively impact aquatic habitats and aquatic communities by consuming substantial amounts of aquatic vegetation and increasing turbidity. Numerous fisheries techniques have been used in an attempt to control or eradicate Grass Carp, including electrofishing. However, electrofishing efficiency for Grass Carp has been variable, and optimum electrofishing waveforms and parameters for inducing a capture-prone response have not been determined. The objective of this study was to determine the optimum electrofishing waveforms and parameters to induce a capture-prone response at various water temperatures and conductivities in juvenile Grass Carp in a controlled, laboratory setting. Results indicated that rectangular pulse waveforms with 60 to 100 Hz frequencies were most effective for immobilization of juvenile Grass Carp. All duty cycles tested (20 – 48%) at these frequencies were effective; although at 60 Hz and 80 Hz frequencies, 24% and 30% duty cycles, respectively, may be more effective. Water temperature was positively related to voltage gradient immobilization thresholds whereas ambient water conductivity and fish size were inversely related to voltage gradient immobilization thresholds. This study provides important information to those seeking to control, eradicate, or detect Grass Carp using electrofishing and provides a framework for future studies focusing on adult Grass Carp.

North American Journal of Fisheries Management↗

USGS2018

A bioassay assessment of a zebra mussel (Dreissena polymorpha) eradication treatment

Zebra mussels (Dreissena polymorpha, Pallas, 1771) are an aquatic invasive species in the United States, and new infestations of zebra mussels can rapidly expand into dense colonies. Zebra mussels were first reported in Marion Lake, Dakota County, Minnesota, in September 2017, and surveys indicated the infestation was likely isolated near a public boat access. A 2.4-hectare area containing the known zebra mussel infestation was enclosed and treated by area resource managers for 9 days with EarthTec QZ (target concentration: 0.5 milligrams per liter as copper), a copper-based molluscicide, to eradicate the zebra mussels. Researchers led an onsite bioassay to provide an estimate of the treatment efficacy within the enclosure. The bioassay was conducted in a mobile assay trailer that received a continuous flow of treated lake water. Bioassay tanks (n=9; 350 liters) within the trailer were stocked with zebra mussels (25 mussels per containment bag; 7 bags per tank) collected from White Bear Lake, Ramsey County, Minn. Mortality in the treated bioassay tanks reached a mean of 99 percent (95-percent confidence interval: 98–100 percent), there were no mortalities in the control tanks. However, a predictive model produced for timely delivery to area resource managers indicated zebra mussel mortality within the treated enclosure may have been as low as 85 percent. Onsite bioassays are a viable and important tool for treatment evaluation particularly in newly infested waterbodies with low zebra mussel densities.

Minnesota↗

USGS2018

Exposure-related effects of Zequanox on juvenile lake sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush)

The environmental fate, persistence, and non-target animal impacts of traditional molluscicides for zebra, Dreissena polymorpha , and quagga, D. bugensis , mussel control led to the development of the biomolluscicide Zequanox. Although previous research has demonstrated the specificity of Zequanox, one study indicated sensitivity of salmonids and lake sturgeon, Acipenser fulvescens , following non-label compliant exposures to Zequanox. This study was conducted to evaluate sublethal and lethal impacts of Zequanox exposure on juvenile lake sturgeon and lake trout, Salvelinus namaycush , following applications that were conducted in a manner consistent with the Zequanox product label. Fish were exposed to 50 or 100 mg/L of Zequanox as active ingredient for 8 h and then held for 33 d to evaluate latent impacts. No acute mortality was observed in either species; however, significant latent mortality (P < 0.01, df = 9; 46.2%) was observed in lake trout that were exposed to the highest dose of Zequanox. Statistically significant (P < 0.03, df = 9), but biologically minimal differences were observed in the weight (range 20.17 to 21.49 g) of surviving lake sturgeon at the termination of the 33 d post-exposure observation period. Statistically significant (P < 0.05, df = 9) and biologically considerable differences were observed in the weight (range 6.19 to 9.55 g) of surviving lake trout at the termination of the 33 d post-exposure observation period. Histologic evaluation of lake trout gastrointestinal tracts suggests that the mode of action in lake trout is different from the mode of action that induces zebra and quagga mussel mortality. Further research could determine the sensitivity of other salmonid species to Zequanox and determine if native fish will avoid Zequanox treated water.

Management of Biological Invasions↗

USGS2018

Effects of temperature and exposure duration on four potential rapid-response tools for zebra mussel (Dreissena polymorpha) eradication

Zebra mussels (Dreissena polymorpha) have continued their spread within inland lakes and rivers in North America despite diligent containment and decontamination efforts by natural resource agencies and other stakeholders. Identification of newly infested waterways by early detection surveillance programs allows for rapid response zebra mussel eradication treatments in some situations. Previous eradication treatments have occurred over a broad range of water temperatures which have influenced the efficacy of molluscicides. Natural resource managers will benefit from knowledge regarding the impacts of water temperature and exposure duration on the toxicity of molluscicides to zebra mussels. In particular, temperature specific data are needed to inform the selection of an effective molluscicide and the proper dose that will induce 100% zebra mussel mortality. We evaluated the influences of temperature and exposure duration on the toxicity of two U.S. EPA-registered (EarthTec QZ and Zequanox) and two nonregistered (niclosamide and potassium chloride) molluscicides to zebra mussels at water temperatures of 7, 12, 17, and 22 °C. Our results indicate that treatment options for the eradication of zebra mussels in waters ≤ 12 °C include 336 h or longer treatments with EarthTec QZ and KCl as well as treatments with niclosamide ≥ 24 h in duration. In waters ≥ 17 °C, multiple toxicant and exposure duration combinations are potentially effective for zebra mussel eradication. On-site or in situ zebra mussel bioassays are a useful tool for the evaluation of treatment efficacy.

Management of Biological Invasions↗

USGS2017

Effects of the biopesticide Zequanox® on reproduction and early development of the fathead minnow ( Pimephales promelas )

The biopesticide, Zequanox®, is registered for dreissenid mussel control in open water systems in the United States. Previous toxicity trials with nontarget organisms, including several young-of-the-year fish species and invertebrates, demonstrated selectivity of Zequanox for dreissenid mussels, but data are lacking on the treatment-related effects on reproduction and early life stage development of fish. The present study evaluated the effects of Zequanox on spawning and early life stages of the fathead minnow, Pimephales promelas , after exposure to the maximum approved concentration [100 mg active ingredient (AI)/L] and exposure duration (8h) for open water application. The results showed no significant treatment-related effect of Zequanox on survival, condition, or cumulative egg production (21 d) in adult fathead minnow. Eggs (≤24 h old) exposed to Zequanox developed to the eyed-stage at a similar rate to that of untreated eggs. Additionally, Zequanox did not have a significant effect on survival and growth (90 d) of newly hatched larvae (≤24-h old). Zequanox may be an option for control of dreissenid mussels in localized open water habitats where concerns exist regarding reproduction and recruitment of cyprinids and related species.

Management of Biological Invasions↗

USGS2017

Use of alternating and pulsed direct current electrified fields for zebra mussel control

Alternatives to chemicals for controlling dreissenid mussels are desirable for environmental compatibility, but few alternatives exist. Previous studies have evaluated the use of electrified fields for stunning and/or killing planktonic life stages of dreissenid mussels, however, the available literature on the use of electrified fields to control adult dreissenid mussels is limited. We evaluated the effects of sinusoidal alternating current (AC) and 20% duty cycle square-wave pulsed direct current (PDC) exposure on the survival of adult zebra mussels at water temperatures of 10, 15, and 22 °C. Peak voltage gradients of ~ 17 and 30 Vp/cm in the AC and PDC exposures, respectively, were continuously applied for 24, 48, or 72 h. Peak power densities ranged from 77,999 to 107,199 µW/cm 3 in the AC exposures and 245,320 to 313,945 µW/cm 3 in the PDC exposures. The peak dose ranged from 6,739 to 27,298 Joules/cm 3 and 21,306 to 80,941 Joules/cm 3 in the AC and PDC exposures, respectively. The applied power ranged from 16.6 to 68.9 kWh in the AC exposures and from 22.2 to 86.4 kWh in the PDC exposures. Mortality ranged from 2.7 to 92.7% in the AC exposed groups and from 24.0 to 98.7% in PDC exposed groups. Mortality increased with corresponding increases in water temperature and exposure duration, and we observed more zebra mussel mortality in the PDC exposures. Exposures conducted with AC required less of a peak dose (Joules/cm 3 ) but more applied power (kWh) to achieve the same level of adult zebra mussel mortality as corresponding PDC exposures. The results demonstrate that 20% duty cycle square-wave PDC requires less energy than sinusoidal AC to inducing the same level of adult zebra mussel mortality.

Management of Biological Invasions↗

USGS2016

Safety of the molluscicide Zequanox (R) to nontarget macroinvertebrates Gammarus lacustris (Amphipoda: Gammaridae) and Hexagenia spp. (Ephemeroptera: Ephemeridae)

Zequanox® is a commercial formulation of the killed bacterium, Pseudomonas fluorescens (strain CL145A), that was developed to control dreissenid mussels. In 2014, Zequanox became the second product registered by the United States Environmental Protection Agency (USEPA) for use in open water environments as a molluscicide. Previous nontarget studies demonstrated the safety and selectivity of P. fluorescens CL154A, but the database on the toxicity of the formulation (Zequanox) is limited for macroinvertebrate taxa and exposure conditions. We evaluated the safety of Zequanox to the amphipod Gammarus lacustris lacustris , and nymphs of the burrowing mayfly, Hexagenia spp. at the maximum approved concentration (100 mg/L active ingredient, A.I.) and exposure duration (8 h). Survival of animals was assessed after 8 h of exposure and again at 24 and 96 h post-exposure. Histopathology of the digestive tract of control and treated animals was compared at 96 h post-exposure. The results showed no significant effect of Zequanox on survival of either species. Survival of G. lacustris exceeded 85% in all concentrations at all three sampling time points. Survival of Hexagenia spp. ranged from 71% (control) to 91% at 8 h, 89–93% at 24 h post-exposure, and 70–73% at 96 h post-exposure across all treatments. We saw no evidence of pathology in the visceral organs of treated animals. Our results indicate that application of Zequanox at the maximum approved concentration and exposure duration did not cause significant mortality or treatment-related histopathological changes to G. lacustris and Hexagenia spp.

Management of Biological Invasions↗