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Jacqueline MacDonald Gibson

Publications and source records attributed to Jacqueline MacDonald Gibson.

2 recordsLinked to original sources

Transdisciplinary approach to private well research

More than 60 million people in the United States and Canada rely on private drinking water systems (mostly private wells), which fall outside of national regulations for water quality and management. This decentralized infrastructure creates unique challenges. First, fragmented policy oversight leads to inconsistent data availability. Second, population heterogeneity in well users requires accounting for the social determinants of health and behavioral differences. Additionally, the individualized nature of well management creates persistent gaps between knowledge generation and the protective action. A broad goal of private well research is to (1) understand, predict, and prevent potential health problems related to well water consumption and (2) enable individual and collective action to reduce exposures to contaminants in well water. Here, we propose a conceptual model to advance private well research through a transdisciplinary approach that links water sources to health and prioritizes engagement among researchers, communities, and decision makers. We also identify challenges and opportunities aimed at improving data systems, refining hazard and risk assessments, linking interdisciplinary research and practical knowledge, and sustaining long-term partnerships. While our geographic focus is on North America, the principles and strategies outlined are globally relevant to addressing health risks associated with unregulated private drinking water sources.

Environmental Science and Technology↗

Probabilistic analysis showing that a combination of bacteroides and methanobrevibacter source tracking markers is effective for identifying waters contaminated by human fecal pollution

Microbial source tracking assays to identify sources of waterborne contamination typically target genetic markers of host-specific microorganisms. However, no bacterial marker has been shown to be 100% host-specific, and cross-reactivity has been noted in studies evaluating known source samples. Using 485 challenge samples from 20 different human and animal fecal sources, this study evaluated microbial source tracking markers including the Bacteroides HF183 16S rRNA, M. smithii nifH, and Enterococcus esp gene targets that have been proposed as potential indicators of human fecal contamination. Bayes' Theorem was used to calculate the conditional probability that these markers or a combination of markers can correctly identify human sources of fecal pollution. All three human-associated markers were detected in 100% of the sewage samples analyzed. Bacteroides HF183 was the most effective marker for determining whether contamination was specifically from a human source, and greater than 98% certainty that contamination was from a human source was shown when both Bacteroides HF183 and M. smithii nifH markers were present. A high degree of certainty was attained even in cases where the prior probability of human fecal contamination was as low as 8.5%. The combination of Bacteroides HF183 and M. smithii nifH source tracking markers can help identify surface waters impacted by human fecal contamination, information useful for prioritizing restoration activities or assessing health risks from exposure to contaminated waters.

Environmental Science & Technology↗